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ANDHRA PRADESH
Name : J Srinivasa Rao
Designation : Lecturer
Branch : D.A.E.I.E
Institute : Govt. Polytechnic, Kothagudem
Year/Semester : VI Sem
Subject : Analytical Instrumentation
Subject Code : AEI-603
Topic : Analysers
Duration : 100 Min
Sub Topic : Spectrofluriometers
Teaching Aids : PPT, Pictures
AEI-603.13 To 14 1
Objectives
• Spectrofluriometer
• Principle
• Applications
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RECAP
• Spectroscopy
• UV / Visible spectrophotometer
• I.R. Spectrophotometer
• Flame Photometer
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Flurosence
• When a quanta of light impinges on a molecule, it is
absorbed and an electronics transition to a higher
electronic state takes place.
AEI-603.13 To 14 4
spectrofluriometer
AEI-603.13 To 14 5
SPECTROFLURIOMETER
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Construction & Working of
spectrofluriometers
• Spectrofluriometers uses two Monochromators, one to
supply selectively the excitation radiation and the other
to isolate and analyze fluorescence emission.
AEI-603.13 To 14 8
• The emitted radiation is analyzed by the analyzing
monochromator, which permits only characteristic
sample emission to reach the detector, blocking all
undesired spectral regions.
AEI-603.13 To 14 9
APPLICATIONS
fluorescence
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Quiz
1) The Principle of Spectroflurometer is based on
the principle of
a) Phosphorescence
b) Fluorescence
c) Chemiluminescence
d) All of the above
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2) The fluorescence light is emitted when the
energy transition from a higher energy state to
lower energy state takes place in a time of
10-6
10-8
10-12
10-14
AEI-603.13 To 14 13
3) The detector used for Spectrofluriometers is
1. Photomultiplier tube
2. Bolometer
3. Thermal conductivity detector
4. All the above
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4) The source used for Spectrofluriometers is
a) Deuterium lamp
b) Tungsten lamp
c) Xenon arc lamp
d) Mercury lamp
AEI-603.13 To 14 15
Frequently asked questions
Spectrofluriometers
AEI-603.13 To 14 16