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Planar Chromatography... What you need: a method TLC or HPTLC precoated layers suitable equipment know how
Planar Chromatography...
A method... Developed and validated by CAMAG
CAMAG Laboratory
www.camag.com
www.camag-laboratory.com
Planar Chromatography... What you need : a method TLC or HPTLC precoated layers suitable equipment know how
Planar Chromatography...
TLC/HPTLC precoated layers available at CAMAG or at your lab supplier
TLC
10 - 15 m wide
HPTLC
5 - 7 m narrow
Layer thickness:
Migration distance:
250 m
100 - 150 mm
100, 200 m
36 - 72 30 - 70 mm
Migration time:
Solvent use: Detection limit:
30 - 200 min
50 ml Abs 100 - 1000 ng Fluor 1 - 100 ng
3 - 20 min
5 - 10 ml 10 - 100 ng 0,1 - 10 ng
Comparison TLC-HPTLC
TLC plate 20 x 20 cm
12 cm migration 25 mL solvent 32 min.
HPTLC plate 20 x 10 cm
7 cm migration 10 mL solvent 10 min.
Flavonoids (Ph.Eur.4)
Planar Chromatography... What you need: a method TLC or HPTLC precoated layers suitable equipment know how
Planar Chromatography
Sample application Chromatogram development Post-Chrom. Derivatization Detection & quantitative evaluation Documentation
Planar Chromatography
Sample application Sample application is the most critical step in the TLC procedure. Errors which occur during sample application, cannot be compensated for in chromatogram development or evaluation.
Planar Chromatography...
step by step
Sample Application
Sample Application
Spot versus Band application
Nanomat
volume 1L 2L
preparative separation
LINOMAT
Linomat 5
Plate holder
Plate holder
Display
Planar Chromatography... step by step Sample Application Chromatogram Development Evaluation Documentation
Chromatogram development
Manual separation via Glass tank
Automatic isocratic separation via ADC Fully automatic gradient separation via AMD2
ADC2
Chromatogram development with standardized conditions in a conventional glass tank
fully automatic saturation controlled humidity controlled
unsurpassed reproducible
qualifiable IQ/OQ
TLC -sprayer
Planar Chromatography... step by step Sample Application Chromatogram Development Evaluation Documentation
Densitometry
TLC Scanner 3
Densitometry
Spectrum Scan
Display of the selected spectra
Spectrum Library
Analysis spectrum compared with a spectrum from the library
Planar Chromatography... step by step Sample Application Chromatogram Development Evaluation Documentation
TLC Visualizer
Design has been enhanced to further improve the homogeneity of the illumination of the plate: 254 nm, short wave UV - Reflectance 366 nm, long wave UV - Reflectance White light - Reflectance and/or Transmission. Ergonomic design: Easy access in to the illumination compartment via large sliding door. separate viewing window with UV-protection Timer for automatic switch-off of the light sources
Manual Optimization
If desired, a region of interest can be defined, in which the brightest spot is automatically found.
Planar Chromatography...
is GLP/GMP compliant: All significant parameters of a TLC method are recorded and electronically stored.
Planar Chromatography... What you need: a method TLC or HPTLC precoated layers suitable equipment know how
Planar Chromatography...
Know How... Application Lab Training courses individual Training
Planar Chromatography...
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CAMAG
Muttenz / Switzerland www.camag.ch
Conclusion ...
..... no laboratory is
complete without a