Experimental Design for the Effect of Inorganic Compound in the Aerobic Granular Sludge Development

How to produce aerobic granular with high nutrient removal efficiency

How to improve biogranulation enhancement

What is the best conditions to optimize aerobic granulation system

What other things can be used for enhancement

In Reactor Granulation enhancement

1. Addition of inert nuclei, adsorbents, synthetic and

natural polymers powder activated carbon (PAC), Zeolite, limestone, polyacryl-amide (synthetic polymer), chitosan (natural polymer) 2. Addition of divalent ions calcium Ca2+,magnesium Mg2+ ,iron Fe2+ (Vlyssides 2009) 3. Influence of pulsation influent to promote hydrodynamic stress 4. Enhancement of substrate utilizing population glucose, sucrose and lactate (Britz 2004)

METHODOLGY
Isolation of microbial from waste water sludge

The selection of type enhancement especially in inorganic matter

Identification of significant effecting variables to optimize performance using experimental design (response surface method)

Characteristics of the Extracellular Polymeric Substance and protein presents on the granulation

Objectives:
To develop aerobic granular sludge by addition of nuclei inert and divalent ions as enhancement in sequencing batch reactor system with the use of dye synthetic wastewater. To characterize the physical, chemical and biological properties of the developed aerobic granular sludge from the enhancement processes To investigate the properties of alginate-like exopolysaccharides isolated from aerobic granular sludge. To select the best conditions to optimize the enhancement process for the granulation development using experimental design

PROJECT ACTIVITIES 1. Literature studies 2. Methodology and Framework Design 3. Identification variables that are expected to affect granulation development y Experimental design using Factorial Design y Inorganic variables (activated carbon, zeolite, palm oil clinker etc) y Responses (coaggregation, surface adsorbility, settleability, permeability, porosity, productionof soluble and soluble EPS, Alginate- like- Exopolysaccharide (ALE), Protein content etc.

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4. Development of mathematical modeling Response Surface Methodology (RSM) Inorganic variables (activated carbon, zeolite, palm oil clinker etc) Responses (coaggregation, surface adsorbility, settleability, permeability, porosity, productionof soluble and soluble EPS, Alginate- like- Exopolysaccharide (ALE), Protein content etc.

5.Optimization study 6. Data Analysis 7. Report Writing Technology Transfer Activities Attending workshop/seminar/conferences O

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