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GENERA:
TREPONEMA & BORREILIA
Prof. Khalifa Sifaw Ghenghesh
Unicellularhelical or spiral rod-
shaped spirochaetes.
Actively motile
Cardiovascular
syphilis
Gummatous syphilis
Histopathology showing Treponema
pallidum spirochetes.
Modified Steiner silver stain.
Treponema pallidum
darkfield preparation
Light microscope pictures showing
tissue infected with the spirochete
Treponema pallidum, the causative
agent of syphilis.
Treponema organisms stained by
fluorescent-tagged antibodies.
Treponema pertenue
– Yaws
– Rural population in subtropical countries
– Non-venereal, after contact of traumatized skin
with exudate from early yaws lesion
– Primary yaws (3-5 weeks) > lesions on the legs
>> papular lesions >> enlarge erode and heal
spontaneously within 6 months > may erupt
weeks or months later.
– Secondary lesions > bones (fingers, long bones
and jaw)
– Late yaws > cutaneous plaques and ulcers and
thickening of the skin on the palms and soles of
the feet.
No neurological and cardiovascular damage
– No congenital yaws
Treponema endemicum
– Bejel (endemic syphilis)
– Non-venereal, affects mainly children rural
populations in Africa, western Asia and Australia
– Direct person to person contact and by sharing
contaminated eating and drinking utensils
– Initial lesion > oral
– Secondary lesions > oropharyngeal mucous
patches, condyloma lata and periostitis
– Late lesion > gummata in the skin, nasopharynx
and bones
No neurological and cardiovascular damage
– congenital bejel is rare
Treponema carateum
Pinta
Rural regions of Mexico, Central
America and Colombia
Confined to the skin
Non-destructive lesion but cause
disfigurement
Direct contact with infectious lesions
resulting in depigmented lesions
which are characteristic of late stages
of pinta with no serious harm
LABORATORY DIGNOSIS
Direct Microscopy
– Specimen: fresh exudate from primary
or secondary lesions
– Examine with dark-ground or phase
contrast microscopy
Serological Tests
- T. pallidum infection > 2 types of Abs
– Specific Abs against polypeptide Ags of
the bacterium
– Non-specific Abs reacts with a non-
treponemal Ag > Cardiolipin
1. Non-Specific Serological Test
Late Syphilis
– Aqueous benzylpenicillin
CONTROL
Treating
index cases and any
known contacts
Borrelia
Gram-negative
Characterized clinically by
recurrent periods of fever and
spirochaetaemia
Disease occur world-wide
Borrelia recurrentis
Causeepidemic or louse-borne
Relapsing fever
– An obligate human pathogen
– Person-to-person transmission by
the body louse Pediculus humanus
Other Borrelia
B. duttoni
B. hermsii
B. parkeri
B. turicatae
– Peripheral blood
Thick or thin blood smears
stained with Giemsa, acridine
orange or other stains
Serological tests