Professional Documents
Culture Documents
1. Skeletal
2. Cardiac
3. Smooth
• Skeletal muscle
– attaches to bone, skin or fascia
– striated with light & dark bands visible with scope
– voluntary control of contraction & relaxation
10-1
• Cardiac muscle
– striated in appearance
– involuntary control
– autorhythmic because of built in pacemaker
10-2
• Smooth muscle
– attached to hair follicles in skin
– in walls of hollow organs - blood vessels & GI
– nonstriated in appearance
– involuntary
– autorhythmic
10-3
Functions of Muscle Tissue
• Producing body movements
• Stabilizing body positions
• Regulating organ volumes
– bands of smooth muscle called sphincters
• Movement of substances within the body
– blood, lymph, urine, air, food and fluids, sperm
• Producing heat
– involuntary contractions of skeletal muscle (shivering)
10-4
Properties of Muscle Tissue
• Excitability - respond to chemicals released from
nerve cells
• Conductivity - ability to propagate electrical
signals over membrane
• Contractility - ability to shorten and generate force
- 2 types of shortening: isometric and isotonic
• Extensibility - ability to be stretched without being
damaged
• Elasticity - ability to return to original shape after
being stretched
10-5
Skeletal Muscle - Connective Tissue
• Superficial fascia is loose connective tissue & fat
underlying the skin, path for BV & nerves
• Deep fascia = dense irregular connective tissue around
muscles with similar functions, isolates muscles from
body allowing movement
• Connective tissue components of the muscle include
– epimysium = surrounds the whole muscle
– perimysium = surrounds bundles (fascicles) of 10-100
muscle cells
– endomysium = separates individual muscle cells
• All these connective tissue layers extend beyond
the muscle belly to form the tendon
10-6
10-7
Connective Tissue Components
10-8
Nerve and Blood Supply
• Each skeletal muscle is supplied by a nerve,
artery and vein, and a rich capillary bed.
• Each motor neuron supplies multiple muscle cells
(neuromuscular junction = NMJ)
• Each muscle cell is supplied by one motor neuron
terminal branch and is in contact with one or two
capillaries.
– nerve fibers & capillaries are found in the
endomysium between individual cells
10-9
Fusion of Myoblasts into Muscle Fibers
• Every mature muscle cell develops from 100 myoblasts that fuse
together in the fetus. (multinucleated)
• Mature muscle cells can not divide
• Muscle growth is a result of cellular enlargement (hypertrophy) &
not cell division (hyperplasia)
• Satellite cells retain the ability to regenerate new muscle fibers.
10-10
Muscle Fiber = Myofiber
10-15
Filaments and the Sarcomere
• Thick and thin filaments overlap each other in
a pattern that creates striations (light I bands
and dark A bands)
• The I band region contains only thin filaments.
• They are arranged in compartments called
sarcomeres, separated by Z discs.
• In the overlap region, six thin filaments
surround each thick filament
10-16
Overlap of Thick & Thin Myofilaments
within a Myofibril
10-19
The Proteins of Muscle
• Myofibrils are built of 3 kinds of protein
– contractile proteins
• myosin and actin
– regulatory proteins which turn contraction on & off
• troponin and tropomyosin
– structural proteins which provide proper alignment,
elasticity and extensibility
• titin, myomesin, nebulin and dystrophin
10-20
The Proteins of Muscle - Myosin
10-21
The Proteins of Muscle - Actin
10-22
Structure of Actin and Myosin
10-23
10-24
The Proteins of Muscle - Titin
• Titin anchors thick filament to the M line and extends to the Z disc.
• Titan is filamentous, springy; maintains side-by-side orientation of
sliding filaments
• The portion of the molecule between the Z disc and the end of the
thick filament can stretch to 4 times its resting length and spring
back unharmed.
• Role in recovery of the muscle from being stretched, prevents
overextension, maintains the position of A band in the center of the
sarcomere. 10-25
Other Structural Proteins
10-26
Components of Sarcomeres
10-27
Sliding Filament Model
10-28
Sliding Filament Mechanism Of Contraction
• Myosin cross bridges
pull on thin filaments
• Thin filaments slide
inward (toward M line)
• Z Discs come toward
each other
• Sarcomeres shorten.The
muscle fiber shortens. The
muscle shortens
• Notice : Thick & thin
filaments do not change in
length!!
10-29
Sarcomere Shortening
10-30
Sarcomere shortening
10-31
How Does Contraction Begin?
• Nerve impulse reaches an axon terminal &
synaptic vesicles release acetylcholine (ACh)
• ACh diffuses to receptors on the sarcolemma &
Na+ channels open, Na+ rushes into the cell
• A muscle action potential spreads over
sarcolemma and down into the transverse tubules
• SR releases Ca+2 into the sarcoplasm
• Ca+2 binds to troponin & causes troponin-
tropomyosin complex to move & reveal myosin
binding sites on actin - contraction cycle begins
10-32
Contraction Cycle
• Repeating sequence of events that cause the
thick & thin filaments to move past each other.
• 4 steps to contraction cycle
– ATP hydrolysis
– attachment of myosin to actin to form crossbridges
– power stroke
– detachment of myosin from actin
• Cycle keeps repeating as long as there is ATP
available & high Ca+2 level near thin filament
10-33
ATP and Myosin
• Myosin heads are activated by ATP
• Activated heads attach to actin & pull (power stroke)
• ADP is released.
• Thin filaments slide past the thick filaments
• ATP binds to myosin head & detaches it from actin
• All of these steps repeat over and over
– if ATP is available &
– Ca+2 level near the troponin-tropomyosin complex is high
10-34
Steps in the Contraction Cycle
10-35
Cross-Bridge Movement
10-36
Breakdown of ATP and Cross Bridge
10-37
Action Potentials and Muscle Contraction
10-38
Action potential along the T
tubules opens Ca channels on
the SR.
T-tubules and SR are physically
linked by a membrane protein –
RYANODINE RECEPTOR
which also functions as a Ca
channel. The ryanodine
receptor comes in contact with
T-tubule membrane proteins –
dihydropyridine (DHP)
receptors. DHPs function as
voltage sensors. Voltage
change alters the DHPs
conformation which opens the
ryanodine receptors
10-39
Action Potentials and Muscle Contrac
10-40
Relaxation
• Muscle action potential ceases
• Acetylcholinesterase (AChE) breaks down ACh
within the synaptic cleft
• Ca+2 release channels on the SR close
• Active transport pumps Ca2+ back into storage in
the sarcoplasmic reticulum
• Calcium-binding protein (calsequestrin) helps
hold Ca+2 in SR (Ca+2 concentration is 10,000 times
higher in SR than in cytosol)
• Tropomyosin-troponin complex recovers binding
site on the actin 10-41
Neuromuscular Junction (NMJ) or Synapse
• Synapse or NMJ
– Presynaptic terminal
– Synaptic cleft
– Postsynaptic membrane or motor end-plate
• Synaptic vesicles
– Acetylcholine: Neurotransmitter
– Acetylcholinesterase: A degrading enzyme in synaptic cleft 10-43
Ion Channels
• Types
– Ligand-gated
• Example:
neurotransmitters
– Voltage-gated
• Open and close in
response to small
voltage changes across
plasma membrane
10-44
Structures of NMJ Region
• Synaptic end bulbs are swellings of
axon terminals
10-45
Function of Neuromuscular
Junction
10-46
Function of Neuromuscular Junction
10-47
Events Occurring After a Nerve Signal
• Arrival of nerve impulse at nerve terminal causes release of
ACh from synaptic vesicles
• ACh binds to receptors on muscle motor end plate opening the
gated ion channels so that Na+ can rush into the muscle cell
• Inside of muscle cell becomes more positive, triggering a
muscle action potential that travels over the cell and down the
T tubules
• The release of Ca+2 from the SR is triggered and the muscle cell
will shorten & generate force
• Acetylcholinesterase breaks down the ACh attached to the
receptors on the motor end plate so the muscle action potential
will cease and the muscle cell will relax.
10-48
Pharmacology of the NMJ
• Botulinum toxin blocks release of neurotransmitter at the
NMJ so muscle contraction can not occur
– bacteria found in improperly canned food
– death occurs from paralysis of the diaphragm
• Curare (plant poison used for arrows)
– causes muscle paralysis by blocking the ACh receptors
– used to relax muscle during surgery
• Neostigmine (anti-cholinesterase agent)
– blocks removal of ACh from receptors - strengthens weak
muscle contractions (as in myasthenia gravis)
– also an antidote for curare after surgery is finished
10-49
Excitation - Contraction Coupling
All the steps that occur from the muscle action potential reaching the T tubule to contraction of the muscle fiber.
•
10-50
Overview: From Start to Finish
• Nerve ending
• Neurotransmittor
• Muscle membrane
• Stored Ca+2
• ATP
• Muscle proteins
10-51
Rigor Mortis
• Rigor mortis is a state of muscular rigidity that
begins 3-4 hours after death and lasts about 24
hours
• After death, Ca+2 ions leak out of the SR and allow
myosin heads to bind to actin
• Since ATP synthesis has ceased, crossbridges
cannot detach from actin until proteolytic enzymes
begin to digest the decomposing cells.
10-52
Length of Muscle Fibers
• Optimal overlap of thick & thin filaments
– produces greatest number of crossbridges and the
greatest amount of tension
• Overstretching muscle (past optimal length)
– fewer cross bridges exist & less force is produced
• If muscle is overly shortened (less than optimal)
– fewer cross bridges exist & less force is produced
– thick filaments crumpled by Z discs
• Normally
– resting muscle length remains between 70 to 130% of
the optimum
10-53
10-54
Length Tension Curve
• Graph of Force of contraction
(Tension) versus Length of
sarcomere
• Optimal overlap at the top
of the graph
• When the cell is too stretched
little force is produced
• When the cell is too short, again
little force is produced
10-55
Muscle Metabolism
Production of ATP in Muscle Fibers
• Muscle uses ATP at a great rate when active
• Sarcoplasmic ATP only lasts for few seconds
• 3 sources of ATP production within muscle
– creatine phosphate
– anaerobic cellular respiration
– aerobic cellular respiration
10-56
Energy Sources
• ATP provides immediate energy for muscle
contractions from 3 sources
– Creatine phosphate
• During resting conditions stores energy to synthesize ATP
– Anaerobic respiration
• Occurs in absence of oxygen and results in breakdown of glucose
to yield ATP and lactic acid
– Aerobic respiration
• Requires oxygen and breaks down glucose to produce ATP,
carbon dioxide and water
• More efficient than anaerobic
10-57
Creatine Phosphate
• Excess ATP within resting muscle used to form creatine
phosphate
• Creatine phosphate is 3-6
times more plentiful
than ATP within muscle
• Its quick breakdown
provides energy for
creation of ATP
• Sustains maximal contraction for 15 sec (used for 100
meter dash).
• Athletes tried creatine supplementation
– gain muscle mass but shut down bodies own synthesis (safety?)
10-58
Anaerobic Cellular Respiration
• ATP produced from glucose
breakdown into pyruvic acid
during glycolysis
– if no O2 present
• pyruvic converted to lactic acid
which diffuses into the blood
• Glycolysis can continue
anaerobically to provide ATP
for 30 to 40 seconds of
maximal activity (200 meter
race)
10-59
Aerobic Cellular Respiration
10-61
Muscle Fatigue
• Inability to contract after prolonged activity
– central fatigue is feeling of tiredness and a
desire to stop (protective mechanism)
– depletion of creatine phosphate
– decline of Ca+2 within the sarcoplasm
• Factors that contribute to muscle fatigue
– insufficient oxygen or glycogen
– buildup of lactic acid and ADP, decrease in pH
– insufficient release of acetylcholine from motor
neurons, lack of Ca+2
10-62
Oxygen Consumption after Exercise
Oxygen Debt
• Muscle tissue has two sources of oxygen.
– diffuses in from the blood
– released by myoglobin inside muscle fibers
• Aerobic system requires O2 to produce ATP
needed for prolonged activity
– increased breathing effort during exercise
• Recovery oxygen uptake
– elevated oxygen use after exercise (oxygen debt)
– lactic acid is converted back to pyruvic acid
– elevated body temperature increases rate of all
metabolic reactions
10-63
The Motor Unit
• Motor unit = one somatic motor neuron & all the skeletal muscle cells
(fibers) it stimulates
– muscle fibers normally scattered throughout belly of muscle
– One nerve cell supplies on average 150 muscle cells that all contract in unison.
• Total strength of a contraction depends on how many motor units are activated &
how large the motor units are
10-64
Stimulus Strength and Muscle
Contraction
• All-or-none law for muscle
fibers
– Contraction of equal force in
response to each action
potential
• Sub-threshold stimulus
• Threshold stimulus
• Stronger than threshold
• Graded response for whole
muscles
– Strength of contractions range
from weak to strong depending
on stimulus strength (frequency
of action potentials)
10-65
Twitch Contraction
10-69
Complete and Incomplete Tetanus
• Unfused tetanus
– if stimulate at 20-30 times/second, there will be only partial
relaxation between stimuli
• Fused tetanus
– if stimulate at 80-100 times/second, a sustained contraction
with no relaxation between stimuli will result 10-70
Explanation of Summation & Tetanus
10-71
Motor Unit Recruitment
‘Spatial Summation’
• Motor units in a whole muscle fire asynchronously
– some fibers are active others are relaxed
– delays muscle fatigue so contraction can be sustained
• Produces smooth muscular contraction
– not series of jerky movements
• Precise movements require smaller contractions
– motor units must be smaller (fewer fibers/nerve)
• Large motor units are active when greater tension is
needed
10-72
Multiple Motor Unit Summation
10-74
Types of Muscle Contractions
• Isometric (static): No change in length while tension
may change
– Postural muscles of body
• Isotonic (dynamic): Change in length with constant
tension
– Concentric: Overcomes opposing resistance and muscle
shortens
– Eccentric: Tension maintained but muscle lengthens
– Isokinetic: Maximal tension during contraction at
constant speed over full range of motion
• Muscle tone: Constant tension by muscles for long
periods of time
10-75
Isotonic and Isometric Contraction
10-78
Size Principle
of motor unit recruitment
The smallest motor units are always recruited first,
and recruitment proceeds with successively larger
motor units as more force is needed by the muscle.
Smallest motor units are recruited first because they
have the smallest neuronal somas (cell bodies),
which require less stimulation to fire action
potentials. In addition, small motor units have
smaller muscle fibers, which generate the least
amount of force.
10-79
• Active versus passive tension
Passive = the tension in the muscle present before
contraction;
Active = the tension developed due to the
contraction
• Velocity of muscle shortening decreases with
increased load – at maximal load, the velocity is zero =
isometric contraction
10-85
Classification of Muscle Fibers
10-86
Shortening velocity dependent on ATPase activity
Different myosin heavy chains (MHCs) have different ATPase activities.
There are at least 7 separate skeletal muscle MHC genes…arranged in series
on chromosome 17.
Two cardiac MHC genes located in tandem on chromosome 14.
The slow β cardiac MHC is the predominant gene expressed in slow fibers
of mammals.
Succinate dehy-
drogenase (SDH)
activity:
Low activity light
High activity dark
Control 12-weeks
treadmill running 10-91
Images courtesy of John Faulkner and Timothy White
Strength training
Early gains in strength appear to be predominantly
due to neural factors…optimizing recruitment
patterns.
10-92
Disuse causes atrophy - USE IT OR LOSE IT!
Individual fiber atrophy (loss of myofibrils) with no loss in fibers.
ATPase activity:
Control Prolonged
bed rest 10-93
Images courtesy of John Faulkner
Anabolic Steroids
• Similar to testosterone
• Increases muscle size, strength, and endurance
• Many very serious side effects
– liver cancer
– kidney damage
– heart disease
– mood swings “-roid rage”
– facial hair & voice deepening in females
– atrophy of testicles & baldness in males
10-94
Regulation of Contraction
• Regulation of contraction due to
– nerve signals from somatic and autonomic nervous
system
– changes in local conditions (pH, O2, CO2, temperature &
ionic concentrations)
– hormones (epinephrine - relaxes muscle in airways &
some blood vessels)
• Stress-relaxation response
– when stretched, initially contracts & then tension
decreases to what is needed
– stretch hollow organs as they fill & yet pressure
remains fairly constant
– when emptied, muscle rebounds & walls firm up
10-95
Regeneration of Muscle
• Skeletal muscle fibers cannot divide after 1st year
– growth is enlargement of existing cells
– repair
• satellite cells & bone marrow produce some new cells
• if not enough numbers - fibrosis occurs (most often)
• Cardiac muscle fibers cannot divide or regenerate
– all healing is done by fibrosis (scar formation)
• Smooth muscle fibers (regeneration is possible)
– cells can grow in size (hypertrophy)
– some cells (uterus) can divide (hyperplasia)
– new fibers can form from stem cells in BV walls
10-96
Effects of Aging on Skeletal
Muscle
• Reduced muscle mass
• Increased time for muscle to contract in
response to nervous stimuli
• Reduced stamina
• Increased recovery time
• Loss of muscle fibers
• Decreased density of capillaries in muscle
10-97
Aging and Muscle Tissue
• Skeletal muscle starts to be replaced by fat
beginning at 30
– “use it or lose it”
• Slowing of reflexes & decrease in maximal
strength
• Change in fiber type to slow oxidative fibers may
be due to lack of use or may be result of aging
10-98
Performance
Performance Declines
Declines with
with Aging
Aging
-- despite
despite maintenance
maintenance of
of physical
physical activity
activity
Performance (% of peak)
100
80
60
40
Shotput/Discus
Marathon
20
Basketball (rebounds/game)
0
10 20 30 40 50 60
Age (years)
D.H. Moore (1975) Nature 253:264-265.
NBA Register, 1992-1993 Edition
10-99
Number of motor units declines during aging
- extensor digitorum brevis muscle of human beings
AGE-ASSOCIATED
ATROPHY DUE TO BOTH…
Loss of fibers
(which as yet appears
irreversible).
Motor
AGING
neuron
loss
10-102
Degeneration-regeneration not necessary to provide muscles
protection from contraction-induced injury
Force deficit (% control)
60 non-trained 60
10-105
Muscular Dystrophy:
A frequently fatal disease of muscle deterioration
• Muscular dystrophies have in the past been classified based on subjective and sometimes
subtle differences in clinical presentation, such as age of onset, involvement of particular
muscles, rate of progression of pathology, mode of inheritance.
• Since the discovery of dystrophin, numerous genetic disease loci have been linked to protein
products and to cellular phenotypes, generating models for studying the pathogenesis of the
dystrophies.
DGC (dystroglycan
complex)
dystrophin
dystroglycan (α and
β )
sarcoglycans (α , β ,
γ ,δ )
syntrophins (α , β 1)
dystrobrevins (α , β )
sarcospan
(Some components of
laminin-α 2 (merosin)
the dystrophin glycoprotein
complex are relatively
recent discoveries, so one
cannot assume that all
players are yet known.)
• Skeletal muscles shorten & pull on the bones they are attached to
• Origin is the bone that does not move when muscle shortens
(normally proximal)
• Insertion is the movable bone (some 2 joint muscles)
• Fleshy portion of the muscle in between attachment sites = belly
10-109
Lever Systems and Leverage
• Muscle acts on rigid rods (bone)
that move around a
fixed point called a fulcrum
• Resistance is weight of body
part & perhaps an object
• Effort or load is work done
by muscle contraction
• Mechanical advantage
– the muscle whose attachment is farther from the joint will
produce the most force
– the muscle attaching closer to the joint has the greater range
of motion and the faster the speed it can produce
10-110
First - Class Lever
Fulcrum is between force and resistance
• May or may not produce mechanical
advantage depending on location of
effort & resistance
– if effort is further from fulcrum than
resistance, then a strong resistance can be
moved
– (+) Need small tension to balance weight
– (-) Limitations are how far a load can be
moved and how heavy a load can be
• Head resting on vertebral column
– weight of face is the resistance
– joint between skull & atlas is fulcrum
– posterior neck muscles provide effort
10-111
Second - Class Lever
Resistance is between fulcrum and effort
• Similar to a wheelbarrow
• (+) Always produce mechanical
advantage (muscle tension needed
is less than the resistance)
– Resistance is always closer to
fulcrum than the effort
• (-) Sacrifice speed for force
• (-) Sacrifice distance a load can be
moved (it is less than the distance a
muscle shortens)
• Raising up on your toes
– resistance is body weight
– fulcrum is ball of foot
– effort is contraction of calf
muscles which pull heel up off of
floor
10-112
Third - Class Lever
Force is between fulcrum and resistance
• Most common levers in the body
• (-) Always produce a mechanical
disadvantage
(muscle tension needed is greater
than the resistance)
– effort is always closer to fulcrum
than resistance
• (+) Favors speed and range of
motion over force
• Flexor muscles at the elbow
– resistance is weight in hand
– fulcrum is elbow joint
– effort is contraction of biceps
brachii muscle
10-113
Coordination Within Muscle Groups
• Most movement is the result of several muscle
working at the same time
• Most muscles are arranged in opposing pairs at
joints
– prime mover or agonist contracts to cause the desired
action
– antagonist stretches and yields to prime mover
– synergists contract to stabilize nearby joints
– fixators stabilize the origin of the prime mover
• scapula held steady so deltoid can raise arm
10-114