Professional Documents
Culture Documents
SUBMITTED BY~
Rohan Khulbe(1503031130).
1
Introduction
challenges
Requirements
Instrument Architecture
Characteristics of opamp 657
Requirements of circuit
Current to voltage converter and band pass filter
Problems in circuit
Complete cicuit
Simulated output
Result
Conclusion
Applications
2
High Speed data acquisition architecture is
implemented which is a part of time resolved
fluorescence detection instrument.
High gain
5
6
High gain bandwidth product(1.6GHz)
Slew Rate(700v/us)
7
Convert current to voltage
High Bandwidth
8
Vo=-IsRf 9
10
ICswere not available
Output was coming out negative
For every input output was same
11
12
13
Fluorescence decay data of tris(2,2'-bipyridyl)ruthenium(II)
obtained by gating a PMT
14
The resulting exponential decay curve fit has
a lifetime of 532ns.
Some reported lifetimes in the literature for
an aqueous solvent are 620ns and 469ns .
15
Fluorescence spectroscopy in biological
systems, minerals, chemicals.
Many substances can be identifiable.
Intrinsic fluorophors in cells allow detection
of metabolites, DNA, RNA and other
biological molecules.
16
OPA657 (2008) OPA847 Datasheets, Texas
Instruments, Dallas, TX, USA.
ADS5484/5485 (2011) ADC12D1800
Datasheets, Texas Instruments, Dallas, TX,
USA.
Lakowicz JR (2006) Principles of fluorescence
spectroscopy. (3rd Edn), Springer
Science+Business Media, New York, USA.
17
18