Professional Documents
Culture Documents
Equipment : Materials :
• Stirring Rod. •Measuring cup
• Aquadest
• Blender • Gauze
• Diethyl Ether
• Funnel • Filter paper
• Ethyl Acetate
• Separate funnel • Whatman
• HCl 2 N
• Desiccator • Round bottom flask
• Methanol
• Erlenmeyer • Analytical balance
• N-butanol
• Beaker glass • Oven
• Petroleum ether.
• Test tube • Pipette
• Waterbath. • Rotavapor
Sample preparation
Wash the Lidah Mertua leaves
with running water which have
been cut into pieces, and dried Mashed the leaves using a
it by aerating without direct blender.
sunlight for 4 hours and oven
at 50 ° C .
Saponin Identification
Determination of saponin.
After cooling, the petroleum
ether solution is removed and the
1.25 grams of extract is refluxed
residual residue is dissolved in 50
with 50 ml of petroleum ether
ml of ethyl acetate. The solution
at temperature 60-80 ° C for 30
is transferred to a separating
minutes.
funnel then separated from the
ethyl acetate solution.
Discarded the Petroleum ether
solution and dissolved the Dissolved The remaining The remaining
remaining residue in 50 ml of residue with 50 ml of n- evaporation is dissolved
ethyl acetate. The solution butanol 3 times, with 10 ml of methanol
which is transferred to a re s p e c t i ve l y. A l l then the solution is
separating funnel is separated butanolic solutions were dripped into 50 ml of
from the ethyl acetate solution mixed and evaporated at diethyl ether while
s o o n a f t e r . the rotary evaporator. stirring.