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– Two types:
• Humoral- antibody mediated
• Cellular- cell mediated
Immunoglobulin Classes
1. IgG IgG
Function/ Characteristic
– Predominant antibody in subclass
secondary responses IgG1 65% of the total IgG
– only antibody that can •against polysaccharide antigens
IgG2 •important defense against
cross placenta (primarily
encapsulated bacteria
IgG1 and IgG3) effective activator of the
• most abundant IgG3 complement due to its rigid hinge
region
immunoglobulin in
not effective activator of
newborns IgG4 complement due to its compact
structure
Immunoglobulin Classes
2. IgM
– main immunoglobulin produced early in the primary immune
response
– can be produced by a fetus with ongoing infection
– Unconfirmed role
• It is found to bind to basophils and mast cells and activate these
cells to produce antimicrobial factors to participate in the respiratory
immune defense in humans.
Functions of Immunoglobulins
Antibody
Isotype-specific Effector Functions
Isotype
•Opsonization of antigens for phagocytosis by macrophages and
neutrophils
IgG •Activation of the classical pathway of complement
•Neonatal immunity: transfer of maternal antibody across the
placenta and gut
Activation of the classical pathway of complement antigen receptor
IgM
of naive B lymphocytes
•Mucosal Immunity: secretion of IgA into the lumens of the GIT and
respiratory tracts
IgA
•Activation of complement by the lectin pathway or by the
alternative pathway
•Mast cell degranulation (immediate hypersensitivity reactions)
IgE
•Immunity to parasites such as helminths and protozoa
• Secondary response
– Presence of memory B-cells
• inflammation
2. Defense against extracellular microbes and
microbial toxins
Neutralization of Microbes and
Their Toxins
• Most important effect of humoral immunity
• Many microbes have binding surface molecules that
attach to host membrane proteins or lipids.
• Influenza virus - haemagglutinin
• Gram negative bacteria – pili
• steric hindrance
Neutralization against Bacteria
1. Opsonization of microbe
by IgG
2. Binding of opsonized
microbes to phagocyte Fc
receptors
3. Fc receptor signals activate
phagocyte
4. Phagocytosis of microbe
5. Killing of ingested microbe
INTRACELLULAR VS EXTRACELLULAR MODE OF ACTION
• Intracellular Microorganisms
1. Phagocyte activation
2. Production of phagocyte oxidase
3. Catalyses intracellular generation
• Extracellular Microorganisms
1. Activated leukocytes can secrete hydrolytic enzymes into
external milieu
2. Killing of extracellular microbes too large to be phagocytosed
ANTIBODY-DEPENDENT CELL-MEDIATED
CYTOTOXICITY
• The attachement of antibodies to viral proteins on the virus-infected
cell can lead to the interaction of the antibody-coated cells with a
killer cell, leading to lysis.
• Since antibodies are protective, strategies have been developed to
induce their production (Active Immunity) or to administer
preformed antibodies to the host (Passive Immunity).
Reference
• Brooks, G., Carroll, K., Butel, J. Jawetz, Melnick, & Adelberg’s
Medica Microbiology (26th edition). The McGraw-Hill Companies:
USA.
THANK YOU!
Mini Quiz
• Differentiate the five immunoglobulins by
its functions.