1430 Michele Lees Eurofins 1

The latest tools to mitigate the risk of fraud in
the food industry

Dr. Michle Lees
Eurofins Analytics, FRANCE
Email: MicheleLees@eurofins.com
www.eurofins.com
Company Overview
The Eurofins Group is the global leader in the

bioanalytical testing market:
More than 225 laboratories with 23 000 employees
Presence in 39 countries in Europe, the USA, Asia-
Pacific and South America
150,000 different analytical methods
In-depth Industry expertise and rapid reaction
capabilities
Investment in R&D and state-of-the-art equipment
Source presentation EDR: 1-F-MK-PRE-0103614

Source modification date: 12/12/2012
European Food Sure 6th Annual European Food Safety & Quality Summit, 26-27 May 2016, Huis ter Duin, Netherlands 2
Laboratories in international locations
Source presentation EDR:-F-MK-PRE-0103614

A unique early warning system
New issues arise all the time...requiring
Closing analytical solutions
gaps in our analytical Even a portfolio of over 150 000 assays does
portfolio not cover all requirements
+
Constant monitoring on a global level
Recognising patterns
allows us to recognise trends and
of potential problems
patterns
Informing customers
Eurofins provides its customers with
on possible concerns information on emerging issues
Source presentation EDR: 1-F-MK-PRE-0103614
Eurofins: ~30 years experience in the development
of analytical methods to detect food fraud
Creation of EUROFINS Laboratory, in Nantes (France)
1987 Pioneer of the SNIF-NMR method to detect wine
enrichment or chaptalisation
Coordination of first main EU-funded projects in this area

FAIM: Food Authenticity Issues and Methodologies 1994
Participation in EU project Food Integrity

2014
Creation of a Knowledge Base to establish the state-
of-the art as regards analytical methods for food
integrity testing
Participation in the Food Fraud Think Tank
2012
The Food Fraud Think Tank
26-28 February 2014

Anaheim, CA, USA
2016
2014
2013
2012 6-8 March 2013

Barcelona, Spain
GFSI: Key elements for food fraud mitigation
Requirements for the food industry
Vulnerability Assessment
Food Fraud Mitigation Plan
Identifying the likelihood
The Eurofins approach of a food
to the prevention of Food
Fraud risk
fraud
An integrated step-wise approach to

mitigate the risk of Food Fraud
Fraud risk
fraud
http://www.foodfraud.org
New USP Food Fraud Database 2.0
Coming in Mid-2016
Expanded records types

and contents
Tailored experience
Dashboard and data
analytics
Hazards ID report
generation to support
FSMA requirements
Source: J. Moore,
Director - Science, Food Standards
US Pharmacopeial Convention
Fraud risk
fraud
Vulnerability Assessment Tools Assess level of risk, assign priority

Example of Food Fraud Vulnerability Assessment
Tool: USP
USP Food Fraud Mitigation Guidance
www.foodfraud.org
Framework for how to design your own
tailored food fraud mitigation system based
on vulnerability and impact (V&I)
assessment
Source: J. Moore, USP
SSAFE Outil pratique pour lvaluation de
Tool: SSAFE
vulnrabilit
Tool created to help companies meet the new GFSI requirements

Applicable to any food business irrespective of size or geographical location
Provides a means of assessing vulnerability to fraud and preparing mitigation
plans
Science-based tool prepared by academia and industry experts
Source: Quincy Lissaur, Executive Director of SSAFE
Tool: SSAFE
vulnrabilit
Preparatio
Series of
Decision 50 n of
Spider
Tree Questions Control
Webs
Plan
FFVA Tool is available at:
http://www.ssafe-food.org Source: Quincy Lissaur

http://www.pwc.com/foodfraud Executive Director of SSAFE
Tool: SSAFE
vulnrabilit
181
1650+ 750+ 51
ONLINE
90 DAYS DOWNLOADS REGISTERED
ASSESSMENTS
COUNTRIES
USERS
COMPLETED
48% 46% 43% 38% 44%
Product Employee Whistle- Direct

Adulteration blowing Suppliers
Monitoring Screening
Products Controls No whistle- Fraud Control
susceptible to No integrity Systems
No fraud screening of blowing
adulteration system Suppliers do
monitoring employees
systems in not have a
place FSMS, are not
audited
externally; or
FSMS
Source: Quincy Lissaur contains no
Executive Director of SSAFE fraud
measures
Fraud risk
fraud
How to choose the right analytical strategy to
reduce the food fraud risk
Dilution
Mislabelling
Adulteration
Substitution
Enhancement
Grey Market
Concealment Counterfeit
How to choose the right analytical strategy to
Visual and/or organoleptic verification

of labels, container, content
Chemical or biomolecular
composition :-
chromatography,
spectroscopy, DNA profile
Stable isotope analysis, trace

element profile
Untargeted or semi-targeted
approach
Applying an untargeted analytical strategy to
Fishing for fraud
The latest untargeted tools treat food products as
fingerprints
Fraud by addition, removal,

modification of chemical
component
Fraud by mislabelling,
substitution of a species,
variety
Example of an untargeted approach: High
Resolution 1H NMR
NMR spectrum of a honey sample
Example ofHow does theapproach:
H NMR honey
High authentication method
1
an untargeted
Resolutionwork?
1
H NMR
Each honey sample = a combination of characteristic peaks

Discrepancies in the profile indicate an anomaly
Statistical treatment of the data can extract even more
information
Numerous applications for untargeted 1H NMR
Eurofins offers this untargeted approach, including experimental

method and comprehensive database, for numerous food products
such as:
Fruit juice Coffee

Arabica v. Robusta
Fruit type Other varieties
Concentrate v. NFC Adulteration
Adulteration
Origin
Wine
Grape variety
Adulteration
Numerous applications for untargeted 1H NMR
and even for high-end products that are top of the fraudsters list:
Caviar
Saffron
Advantages of untargeted 1H NMR
Simple sample preparation
Rapid measurement on a 1H NMR

spectrometer (~20 minutes)
Information rich data processing

and statistical evaluation
Interpretation and
identification of
authenticity markers
Example of a semi-targeted approach: DNA Chip
technology for meat speciation
? Ra Species-specific PCR
Ch
bb t?
ick
it ? a
Go
en
?
To detect 1 particular
Horse ?
species
Duck ?
s e? To detect a large panel

o
Go of expected and/or undesired
rk ? species
Po Beef ?
g ?
Do
Sh
e ep
t/
? Consensus PCR
Ca
Example of a semi-targeted approach: DNA Chip
technology for meat speciation
8 spots per chip:
6 test samples
1 positive control (for all 20 species)
1 negative control
Each spot contains specific hybridization probes

in duplicate, for simultaneous qualitative
detection of up to 20 species
Advantages of the DNA Chip technology for meat
speciation
ONE test can detect up to 20 species in the same sample

High sensitivity : use of mitochondrial DNA targets
Verify that product composition is in accordance with the species
mentioned on the ingredient list
Test for absence of undesired species: horse meat in beef,
ruminants in animal feed,
Search for species for which no other test is currently available
(e.g. rabbit)
The Eurofins approach to the prevention of Food
Fraud
Food Fraud Mitigation

Action Plan
Carry out a diagnostic of the entire
supply chain
Identify vulnerable weak links
Implement mitigation measures
including:
An appropriate testing regime
A suitable supplier audit
strategy
For more information, visit us
at Booth N 2
THANK YOU
www.eurofins.com

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The latest tools to mitigate the risk of fraud in

the food industry

The Eurofins Group is the global leader in the

Source presentation EDR: 1-F-MK-PRE-0103614

Source presentation EDR:-F-MK-PRE-0103614

Coordination of first main EU-funded projects in this area

Participation in EU project Food Integrity

26-28 February 2014

2012 6-8 March 2013

Requirements for the food industry

Food Fraud Mitigation Plan

An integrated step-wise approach to

Expanded records types

Vulnerability Assessment Tools Assess level of risk, assign priority

Source: J. Moore, USP

Tool created to help companies meet the new GFSI requirements

FFVA Tool is available at:

http://www.ssafe-food.org Source: Quincy Lissaur

48% 46% 43% 38% 44%

Product Employee Whistle- Direct

Visual and/or organoleptic verification

Stable isotope analysis, trace

Fishing for fraud

Fraud by addition, removal,

NMR spectrum of a honey sample

Each honey sample = a combination of characteristic peaks

Eurofins offers this untargeted approach, including experimental

Fruit juice Coffee

Simple sample preparation

Rapid measurement on a 1H NMR

Information rich data processing

s e? To detect a large panel

Each spot contains specific hybridization probes

ONE test can detect up to 20 species in the same sample

Food Fraud Mitigation

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