METHODOLOGY

Materials

Prepared
Slides
Sterile lancets Hand soap
Compound Glucometer and
Microscope strips
Cotton balls
 METHODOLOGY

5% glucose Timer
18 oz coke solution

70% Alcohol
Goldfish

Insulin and Beaker Distilled

syringe water
 METHODOLOGY
Methods
Endocrine glands and their histological structures

Histoanalysis
Draw the structures seen
Obtain prepared slides of the under the microscope,
preferred endocrine glands Locate the desired
tissues and cells in indicate the magnification,
each endocrine gland then label.
under a compound
microscope

Prepared Drawing and

slides Labelling
Methods Glucose Tolerance Test METHODOLOGY

Finger prick using sterile

Precautionary lancet: 4 repetitions (1st Application of
measures and prick control; 3 next
requirements of
blood sample on
pricks are after each
the test subjects coke drinks; 30 minutes strips: 30 seconds
interval

Insertion of the
Cleaning and Record glucose strip to
disinfection of the
prick finger with measurement glucometer:
alcohol using cotton and time 10 seconds
swabs and bandage
 Methods METHODOLOGY

Insulin Shock on Goldfish

Materials
Set-up 1
Prepare 2 goldfishes and
3 beaker with different Place one goldfish Set-up 2
solutions: to beaker 1 and
Beaker 1: 100 ml distilled other goldfish to Transfer the
water beaker 2. Record goldfish from
Beaker 2: 100 ml distilled behaviour of both beaker 2 to
water + 500 units insulin
goldfish beaker 3. Record
Beaker 3: 5% glucose
concentration observation.
Histological Structures of Different Glands
Anterior Pituitary Posterior Pituitary
Histological Structures of Different Glands
Thyroid Parathyroid Pancreas

Adrenal Ovaries Testes

 Whatwill participants have to do to
have the OGTT test?

Whatwill this test tell participants

about their health?
 comparing the levels of glucose in the blood
establishing a diagnosis of a mild case of diabetes
type 2 diabetes or pre-diabetes (impaired
glucose tolerance), diabetes in pregnancy

increase of type 2 diabetes in teenagers and

adults
 Initially,
there was no observed
changes in goldfish when it was
placed to the beaker with distilled
water.
But when the little fish was transferred
into the beaker with 500 units of insulin,
it became hyper showing rapid
swimming and excited/frantic body
movements
 Insulin is a hormone secreted by pancreas
allows your body to use suga. Insulin helps
keeps your blood sugar level from getting too
high or to
Of this phenomenon that the externally
applied insulin crosses the fish gill and enters
the circulation of the fish where the insulin then
has its usual physiological effect of lowering the
blood glucose concentration to an extent
which induces a hypoglycemia to the fish
(Munford. Et al.)o low.
 Some groups showed changes in
color and buoyancy to their fish.

Prolonged and too much insulin exposure to the gold fish

may result to hypoglycemic coma.
And in the labarotory practices, the fish could be revived
by subjecting it to glucose concentration Zarrow et al.
As we did at the last part of this activity
 Structures of the Glands
A gland is an organ in an animal's body that
synthesizes substances (such as hormones)
for release into the bloodstream (endocrine
gland) or into cavities inside the body or its
outer surface (exocrine gland). Thus,
structural differentiation of every types of
glands is necessary in production and
secretion of hormones throughout the body.
Structure-Function Relationship
 Glucose Tolerance Test
This test measures how the body handles
sugar. We will be able to tell if a person is
at risk for diabetes or already has it. The
person or his or her parents will be told if
the test shows a blood sugar problem.
 Insulin Shock in Gold Fish
Vice versa, When goldfish are maintained in
glucose solutions, both chronic
hyperglycemia and hepatic fat deposition
are found to result (Schuster-Woldan, 36;
and Sterne, 67). Also, Kohler (63) reported
that the hepatic fat deposition associated
with keeping fish in glucose solutions can be
reversed by adding insulin to that glucose
solution. With such, concentration balance
of glucose and secretion of insulin in the
body is significant in maintaining homeostasis
(sugar concentration).