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SEM 1 2006/2017
PREPARED BY
HJH ROSLINDA SESWOYA
General Requirements
1. Be properly prepared to do the experiment scheduled for that day.
2. There will be no smoking, using CELL PHONES, drinking, chewing gum, or eating in
the laboratory.
3. Do not touch your face, handle contact lenses, apply cosmetics or put your fingers
in your mouth.
4. Always wash your hands after handling viable material.
5. Avoid working alone in the laboratory.
6. Horseplay will not be tolerated. Avoid distracting others that are working.
7. At the end of each lab session, clean your lab bench, put away all materials, and
wash your hands thoroughly before leaving the laboratory. Wastes should be
deposited in appropriate receptacles.
8. Gloves and lab coats are to be removed prior to leaving the laboratory and may not
be worn in non-laboratory areas.
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Personal Protective Equipment
(PPE):
1. Observe all laboratory signs and chemical labels for
required protective equipment.
2. Safety glasses or chemical splash goggles will be worn at
all times when performing laboratory work.
3. Wearing contact lenses is discouraged.
4. Always wear gloves when handling any chemicals or
microorganisms.
5. You are required to wear laboratory coats while working to
prevent contaminating your clothes.
6. Wear shoes that cover the entire foot; NO sandals or open
shoes.
7. Tie back long hair and confine loose clothing to keep away
from flames and chemicals.
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Hazardous Material Safety:
1. Unauthorized experiments are prohibited. Perform the experiments as directed by
your instructor.
2. Never take chemicals, supplies, or equipment out of the laboratory.
3. Students are not allowed to enter the Chemical Stockrooms.
4. Know where the MSDS (Material Safety Data Sheets) are for your chemicals. MSDS
contain information that you will need in case there is an emergency.
5. Never taste or smell a chemical.
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MA01 Total Suspended Solid (TSS):
Weigh the Pour 15 mL of water
Switch on record the sample ( from UTHM
the initial dry lake) on the filter
balance weight of paper and leave it Take the filter paper,
( at least filter until the filtration place on the
30 min paper finishes evaporating dish
before the
test)
Weigh
and
record the Place the
final dry evaporating dish
weight of to cool at room Place the
filter temperature in evaporating disk
paper dessicator inside the drying
oven at 1050C for at
least 1 hour or until
completely dry.
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MA01 Total Suspended Solid (TSS):
For Environmental Engineering Laboratory ( BFC 32501) SEM 1 2016/2017, submit
the lab report by answering the following
Volume
2) In of sample
your opinion, (mL) to discharge the sample
is it possible V used in this test to be
discharged to the river?. Give your reason.
B) Discussion
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MA02 Bacteria Count
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MA02 Bacteria Count
Note Prepare freshly by
All equipment boiling the mixture of:
used in this peptone = 5 g
experiments Inoculate
plate
Beef extract = 3 g
should be containin Agar = 15 g
sterilized in an g Distilled water = 600
autoclave at nutrient mL
agar
1210C for 15 min
Sterile the spreader
Carefull with
each time before
ethanol and
spread the bacteria
Bunsen burner
sample over the agar
Close the plate. by immerse the
Invert the plates spreader in ethanol,
and incubate for 24 tap on the side of the
hours at 300C beaker to remove the
excess ethanol and
After being flame
Close the plate.
incubated for 1 da, Invert the plates
take out the plates and incubate for 24
and count the hours at 300C
colony using colony
counter
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MA02 Bacteria Count
For Environmental Engineering Laboratory ( BFC 32501) SEM 1 2016/2017, submit the
lab report by answering the following
1/100
1/1000
1/10000
1/100000
B) Discussion
2) Between pour plate and spread plate,which method is more flexible to be used as
enumeration procedure for various types of bacteria ? 11
MA03 Biochemical Oxygen Demand (BOD)
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MA03 Biochemical Oxygen Demand (BOD)
Eqn A = minimum waste to be filled in BOD bottle
= [(2 mg/L ) X 300 mL] / 400 mg/L
= 1.5 mL
Eqn B = maximum waste to be filled in BOD bottle
= [(8 mg/L- 1 mg/L ) X 300 mL] / 400 mg/L
= 5.2 mL
Therefore sample size = 1.5 to 5.2 mL (Let say choose 3 mL)
4. Take 5 BOD bottles. Use 3 bottles for sample, and 2 for blank
5. Fill the BOD bottles ( one by one) as below:
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MA03 Biochemical Oxygen Demand (BOD)
13. Apply Eqn C to measure BODX for the sample.
Di = initial DO ( mg/L)
Df = final DO (mg/L) , 5 days or 3 days
P = Volume of sample used / Total volume
Note:
1) DO in blanks should not deplete more than 0.2 mg/L
2) DO in sample should not less than 2 mg/L
3) Final DO in the samples should not less than 1 mg/L
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MA03 Biochemical Oxygen Demand (BOD)
For Environmental Engineering Laboratory ( BFC 32501) SEM 1 2016/2017, submit
the lab report by answering the following
B) Discussion
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MA05 Chemical Oxygen Demand (COD)
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MA05 Chemical Oxygen Demand (COD)
Discussion
3) Is it possible to predict the BOD of the sample using the COD value? If yes,
explain how to do that.
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GOOD LUCK !!!
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