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Biological hydrogen and methane production

from bagasse bioethanol fermentation


residues using a two-stage bioprocess
Hai-Hsuan Chenga, Liang-Ming Whanga,b,c,*, Man-Chien Chunga, Kun-Chi Chana
Department of Environmental Engineering, National Cheng Kung University (NCKU), No. 1, University Road, Tainan 701,
a

Taiwan
bSustainable Environment Research Laboratory (SERL), National Cheng Kung University (NCKU), No. 1, University Road,

Tainan 701, Taiwan


cResearch Center for Energy Technology and Strategy (RCETS), National Cheng Kung University (NCKU), No. 1, Tainan

701, Taiwan

Presented By:
Jasan Robey A. Mangalindand
School of Chemical Engineering and Chemistry, Mapa Institute of Technology, Muralla St., Intramuros, Manila, Philippines
d
BIOHYDROGEN
Ideal bioenergy High specific energy content Generates only water
when burned
Can be produced biologically
SOURCE MATERIALS FOR BIOHYDROGEN PRODUCTION

Municipal waste factions Lipid-extracted microalgae Food waste


(De Gioannis et al., 2013) (Yang et al., 2011) (Jo et al., 2008)

Domestic wastewater Cellulose


(Van Ginkel et al., 2005) (Ratti et al., 2013)

Fermentation

BIOHYDROGEN
SOURCE MATERIALS FOR BIOHYDROGEN PRODUCTION

Municipal waste factions Lipid-extracted microalgae Food waste


(De Gioannis et al., 2013) (Yang et al., 2011) (Jo et al., 2008)

Domestic wastewater Cellulose


(Van Ginkel et al., 2005) (Ratti et al., 2013)

Fermentation

Bagasse Bioethanol
Fermentation Residues (BEFR)

BIOHYDROGEN
OBJECTIVES
Recover bioenergy in the forms of H2 and CH4 through the two-
stage bioprocess
Evaluate the effects of substrate concentration and biomass
concentration on biohydrogen formation
Determine the overall bioenergy recovery and COD removal
efficiency from bagasse BEFR using the two-stage bioprocess
METHODOLOGY
1. Two-stage bioprocess
2. Batch fermentative hydrogen tests
PROCESS DIAGRAM
Bagasse BEFR

Treatment Treatment

Refrigeration

Hydrogen Fermentation Centrifug Glass Bottle Bioreactor


Bioreactor (HFB) e

Refrigeration

Methane Fermentation
Bioreactor (MFB) ANALYSIS
TWO-STAGE BIOPROCESS

Bagasse BEFR Treatmen Treated Refrigerato Treated bagasse


t bagasse r 4C
Growth
nutrients

A
HFB effluent Refrigerato (HFB)
37C ANALYSIS
4C r

B Biomass
(MFB)
ANALYSIS 37C
BATCH FERMENTATIVE HYDROGEN TESTS
N2

Bagasse BEFR Treatmen Treated Seed Centrifug Biomass


t bagasse e from HFB
Growth
nutrients

Glass
bottle
bioreactor

ANALYSIS
ANALYSIS
Soluble components (such as xylose, acetate, butyrate, ethanol,
and butanol)
HPLC (Hitachi D2000 system, Japan) with an ICSep COREGEL-87H3 column
(Transgenomic, USA)
Refraction index detector (Hitachi Chromaster 5450, Japan)
Biogas
Gas chromatograph (China GC 8900, Taipei, Taiwan) equipped with a
thermal conductivity detector (TCD)

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