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bSustainable Environment Research Laboratory (SERL), National Cheng Kung University (NCKU), No. 1, University Road,
701, Taiwan
Presented By:
Jasan Robey A. Mangalindand
School of Chemical Engineering and Chemistry, Mapa Institute of Technology, Muralla St., Intramuros, Manila, Philippines
d
BIOHYDROGEN
Ideal bioenergy High specific energy content Generates only water
when burned
Can be produced biologically
SOURCE MATERIALS FOR BIOHYDROGEN PRODUCTION
Fermentation
BIOHYDROGEN
SOURCE MATERIALS FOR BIOHYDROGEN PRODUCTION
Fermentation
Bagasse Bioethanol
Fermentation Residues (BEFR)
BIOHYDROGEN
OBJECTIVES
Recover bioenergy in the forms of H2 and CH4 through the two-
stage bioprocess
Evaluate the effects of substrate concentration and biomass
concentration on biohydrogen formation
Determine the overall bioenergy recovery and COD removal
efficiency from bagasse BEFR using the two-stage bioprocess
METHODOLOGY
1. Two-stage bioprocess
2. Batch fermentative hydrogen tests
PROCESS DIAGRAM
Bagasse BEFR
Treatment Treatment
Refrigeration
Refrigeration
Methane Fermentation
Bioreactor (MFB) ANALYSIS
TWO-STAGE BIOPROCESS
A
HFB effluent Refrigerato (HFB)
37C ANALYSIS
4C r
B Biomass
(MFB)
ANALYSIS 37C
BATCH FERMENTATIVE HYDROGEN TESTS
N2
Glass
bottle
bioreactor
ANALYSIS
ANALYSIS
Soluble components (such as xylose, acetate, butyrate, ethanol,
and butanol)
HPLC (Hitachi D2000 system, Japan) with an ICSep COREGEL-87H3 column
(Transgenomic, USA)
Refraction index detector (Hitachi Chromaster 5450, Japan)
Biogas
Gas chromatograph (China GC 8900, Taipei, Taiwan) equipped with a
thermal conductivity detector (TCD)