Bacterial Growth and Metabolism

-- Chapter 9, & part of Chapter 14

Learn on your own the

materials in the section growth:
-- Population growth
-- Water and temperature
-- Oxygen and acidity
-- Other factors
(Much of the chapter Self-test
focuses on these topics)

Also read section in Chap 14 on

Microbes and Industry

*** You will not be responsible for all of the

details of the metabolic pathways covered in
this chapter ***

Bacterial Growth and 1

Bacterial Growth and Metabolism
-- Chapter 9

All organisms have

three fundamental requirements

Carbon

Energy

Mineral nutrients

Bacterial Growth and 2

Prokaryotes are much more varied
in their sources of C and energy

Bacterial autotrophs

Phototrophs
-- photosynthetic bacteria

Chemoautotrophs
Lithotrophs
-- mineral oxidizing bacteria

Bacterial Growth and 3

Prokaryotes are much more varied
in their sources of C and energy
Bacterial heterotrophs
Photoheterotrophs
Chemoheterotrophs
saprophytic
parasitic

Generalists vs fastidious bacteria

Bacterial Growth and 4

Use of Microbes in Bioremediation

Toxic waste sites:

PCBs, dioxins, organic solvents, etc

Traditional clean up is expensive & inefficient

(10s to 100s $M)

Bioremediation is better alternative

Less impact

Cheaper

Does not merely move the problem

Bacterial Growth and 5

Cellular Metabolism

Why is this topic of importance? Overview of Cellular Respiration

identification

microbial application

chemotherapies

What happens to organic molecules

that enter a cell?

Enzymatically degraded

molecular building blocks

energy retention

Bacterial Growth and 6

Glycolysis

Converts sugars into Pyruvate

Requires energy investment

Energy yield

ATP

NADH

Glycolysis

Bacterial Growth and 7

Aerobic Cellular Respiration
Transition reaction

Krebs cycle

Electron transport

Energy yield

ATP

NADH

e- Transport

Bacterial Growth and 8

Anaerobic metabolism
Bacterial O2 requirements
Obligate aerobes

Facultative

Microaerophiles

Obligate anaerobes

Fermentation

Functions

End products

Alternative terminal e- acceptors

e.g., CO2, SO4

Bacterial Growth and 9

Industrial Uses of Bacteria
-- chapter 14

Figure 5.12
Other fermentation products

Antibiotics and
pharmaceutics

Bacterial Growth and 10

Beer production
Normal beer: only yeast
boring!

Lambic beer yeast and bacteria!

Yeast CO2 production

Bacterial Growth and 11

Cheese production
Involves bacteria (and sometimes fungi)

Curdling stage

Ripening stage

Bacterial Growth and 12

Autotrophic bacteria
CO2 is carbon source
Energy from light (phototrophs)
or inorganics (chemoautotrophs)

Phototrophs

Purple sulfur bacteria

-- anoxygenic Ps

Cyanobacteria
-- oxygenic Ps

Bacterial Growth and 13

Cyanobacteria
blue-green pigmentation

Habitat Visit NASAa

Stromatolite
Stromatolites Explorer on-line

Oxygenic
Photosynthesis

Bacterial Growth and 14

Purple sulfur bacteria
Photosynthesis

Habitat

Pigmentation Anoxygenic
Photosynthesis

Sulfur granules

Bacterial Growth and 15