Professional Documents
Culture Documents
Current Methods
Full or partial removal of wound pad
Visual Observation
Skin Irritation
Increased chances of infection
Only qualitative information
Collecting biochemical information
Highly invasive
Expensive
The Business Case
We want to build a wound healing system that can monitor the healing
process and providing quantitative data.
Non-invasive
TOF camera
Sensing layer
pH,
metabolites,
oxygen,
Wound
Pad Structure
material
proteins and enzymes
E
E
metabolites and enzymes,
H + , O2
pH Glucose
Depends on time course
Bio-marker to assess the
and wound-stage.
Normal healing -> pH Oxygen wound status.
Infected wounds:
increases during
granulation and concentration of around
decreases afterwards to 0.31.0 mM.
reach a value of 4-6. 1) Controls cell Non-infected wounds:
Impaired healing -> proliferation concentration of around
2) Anti-bacterial 5.07.6 mM.
the pH oscillates between
activities
pH 7-8
3) Creating new blood
vessels
(angiogenesis),
which is essential to
the growth and
survival of repaired
The sensors
Non-invasive
TOF camera
Sensing layer
Parameter
Wound
Fluorescent pH-marker
Wavelength / nm
700
Fluorescence Intensity / a.u.
600
500
400
300
200
100
0
5 5.5 6 6.5 7 7.5 8
pH
Enzimatic glucose sensing
Horseradish peroxidase
GOx, 30C
Ethyl cellulose
PtOEP
1 cm
Alginate gel
Long Luminescence decay times
Luminescence brightness of the probe
Can be detected @ low
concentration
Solubility in the polymeric matrices
Chemical stability and photostability
Commercial or synthetic availability
Oxygen Sensor System
Currently we are also working to precisely position the sensors in the gel
matrix.
Antinode
/2
UV excitation
Sensors
Side view
PZT 15
m
The Lifetime Camera
Non-invasive
TOF camera
Sensing layer
pH,
metabolites,
oxygen,
Wound
Fluorescence lifetime imaging
Phase-resolved
intensity images
3000
2500
Lifetime [ns]
2000
1500
1000
500
0 10 20 30 40 50 60 70 80 90 100
Oxygen O2 Concentration [%]
Testing in Animal Models
Lactate
pH
Glucose
O2
Pig Pig
Aim 1: testing fresh wounds Aim 2: testing infected wounds
Feasibility for wound sensor Feasibility of primary closure
pad Monitoring over time
Feasibility of parameters
Wound model Wound model
Aim 1: Standardized wound on Aim 2: Standardized wound on
dorsum of animal; size 4 x 4cm2; tibia directly above bone; size 4 x
comparison wound pad to 4cm2; infection with S. aureus;
standard wound bandage, comparison wound pad to
histology of wound healing standard wound bandage
https://www.eqalix.com/technology-and-pip
WP5 : In Vivo Evaluation
Treatment groups:
Group 1: Standard of Care
Group 2: Flusitex without molecular sensors (scaffold only)
4 cm
Group 3: Flusitex dressing
4 cm
Wound area
Imaging area
6mm diameter
WP5 : In Vivo Evaluation
Achievements
Development of:
o pH sensor
o biomarker sensors
o oxygen sensor
Future Efforts
FlusiGate
Flawa, Kenzen, Schoeller,
TheranOptics:
Project to develop a ratiometric
sensor for wound monitoring
based on pH responsive dyes
and a point fluorescent detector
FlusiSafe
U-NICA Solutions AG : project to
investigate the potential of the
FLIM imager for anti-
counterfeiting & product
protection
Prof. Bradley Nelson
Dr. Luciano Boesel
Dr. Daniel Ahmed
Dr. Claudio Toncelli
Dr. Chen Xiangzhong
Dr. Alina Osypova
Dr. Guido Panzarasa
Thanks!
Dr. Stefano Cattaneo
Dr. Greta Faccio Mr. Christoph Hofer
Dr. Dagmara Jankowska Dr. Hans-Rudolf Graf
Dr. Bernhard Schaffer