D

N
G
A
N
I
N
L
O
L
I
I
T
C
I
C
N
A
E
R
P
T
M
L
F
X
E
R
O
F
Y
H
R
E
G
V
N
I
O
S
C
U
E
Y
R
B
A.RAGHAVENDRA BT13CME003
PAYAL BHONDE BT13CME019

INTRODUCTION
PENICILLIN G IS ONE OF THE MOST WIDELY USED ANTIBIOTICS BECAUSE OF
BACTERIAL ACTIVITY
BROAD SPECTRUM
LOW TOXICITY
EXCELLENT DISTRIBUTION THROUGHOUT BODY

SOLVENT EXTRACTION IS THE MOSTLY USED INDUSTRIALIZED TECHNIQUE FOR

REMOVAL OF PENICILLIN G
DISADVANTAGES OF SOLVENT EXTRACTION.
LOW EFFICIENCY
HIGH SOLVENT CONSUMPTION
LOSS OF PENICILLIN G

TRADITIONAL LIQUID MEMBRANE PROCESSES ARE NOT APPLICABLE BECAUSE OF

SWELLING OF EMULSION LIQUID MEMBRANE
INSTABILITY OF SUPPORTED LIQUID MEMBRANE
HFRLM TAKES ADVANTAGE OF NON-EQUILIBRIUM MASS TRANSFER.
BY HFRLM SIMULTANEOUS SEPARATION AND CONCENTRATION OF PENICILLIN G IS
CARRIED OUT.

REAGENTS & MATERIALS

FEED PHASE SODIUM SALT OF PENCILLIN G + KH 2PO4 BUFFER
ORGANIC PHASE - DI-N-OCTYLAMINE (DOA) + ISO-OCTANE + KEROSENE
STRIPPING PHASE - K2CO3 AQUEOUS SOLUTION
MEMBRANE HYDROPHOBIC POLYPROPYLENE HOLLOW FIBRES

PRINCIPLE
CARRIER FACILITATED TRANSPORT
HP H+ + PH++ P-+ AO

HPAO

STRIPPING SOLVENT TAKES OUR PENICILLIN G AND REDUCES THE

CONCENTRATION OF PENICILLIN IN ORGANIC SOLVENT WHICH HELPS IN
CONTINUOUS TRANSPORT

NECESSITY OF CASCADE

EFFECT OF PH

EFFECT OF BUFFER SOLUTION

EFFECT OF FEED CONCENTRATION

EFFECT OF CARRIER CONCENTRATION

EFFECT OF FLOWRATE

ADVANTAGES
GOOD STABILITY
HIGH MASS TRANSFER RATE
NO LEAKAGE BETWEEN PHASES
LOW EXTRACTANT CONSUMPTION