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What is Mycology?
Mycology
Fungus Family
yeast
Molds
Unicellular
Budding fungus
Multicellular
Threadlike
Eukaryotic organism
No chlorophyll
Have cell walls
General
Characteristics
Filamentous structure
Produce spores
Grow as saprophytes
Decompose dead organic
material
Classification of Fungi
Zygomycetes
Ascomycetes
Basidiomycetes
Deuteromycetes
Mycoses
Is
Systemic mycoses
Subcutaneous mycoses
Superficial
mycoses
Ringwo
rm
(tinea)
Example
Infects:
ple
exam
Subcutaneous
mycoses
Infects:
Systemic
mycoses
Organs
Invading throughout body
Most damaging
ex
a
m
ple
Specimen collecting
Scraping
Laboratory Diagnosis
Microscopy
Place the specimen on slide
Heat the slide & add 10% KOH
Add stain:
Methylene blue/ lactophenol/ ink
Culturing
Sabouraud dextrose
agar
Cornmeal agar
Serology
Identify the antibodies to specific fungal
antigen on patients serum.
Antigen detection
Finding specific antigen by using specific
antibody
Cutaneous test
Allergy test with fungal antigen
Yeasts
*Candida Albicans
Candida Albicans
Kingdom:Fungi
Phylum:Ascomycota
Subphylum:Saccharomycotina
Class:Saccharomycetes
Order:Saccharomycetales
Family:Saccharomycetaceae
Genus:Candida
Species:C.
albicans
Disease: Candidiasis
Pathogenicity:
* Candidiasis
* Comensal in GIT
* Candida infection-opportunistic,
associated with prolonged
broa-spectrum antibiontic therapy
Treatment:
* Amphotericin B,Caspofungin, or fluconazole for
systemic infections
*Fluconazole or caspofungin for oral or esophageal
infections
* Topical azole for vaginal infections
Dermatophytes
Aspergillus
species
Example of
(Aspergillosis)
Molds
Dermatophytes
Pathogenicity
Cause infections of the skin,hair and nails due to their
ability to obtain nutrients from keratinized material
Do not invade living tissue but colonize the outer
layer of the skin
Morphology & culture
Culture on a small media called Dermatophytes Test
Medium (DTM) @ room temperature:turn bright red
after 10-14 days
Sample
Scraping from nail,hair or skin
Diagnosis
Fungal culture
Microscopic examination(prepare slide)
Prevention
Avoid contact with infected host
Aspergillus species
Pathogenicity
Common contaminants of starchy food
(bread,potatoes)
Produces dry,hydrophobic spores that
are easily inhaled
Cause infection to human and animals
(infection to the external ear,skin
lesions,ulcers)
Colony morphology of
Aspergillus vagentum
Example of
Dimorphic fungi
Histoplasma capsulatum
(histoplasmosis)
Coccidiodes immitis
(coccidiomycosis)
to
e
l
ab
st
i
a
g
e
Fun as y
w
gro oulds
or m
Histoplasma capsulatum
Pathogenicity
Cause histoplasmosis
Breathed in through a persons lungs
Can be serious with infants, people with weakened immune
system or who have chronic disease
Morphology and culture
on Sabouraud dextrose agar @ 25C : white cottony mycelium
after 2 3 weeks
Mold form has spore named tuberculate macroconidium
@ 37C yeast appears : white tan colony
Sample
Urine,
serum,
other
body fluids
Diagnosis
fungal
culture
Fungal stain or blood test
Chest X-ray or computerized tomography (CT) scan
Prevention
Wear
Histoplasma in
yeast form
Spores of
histoplasma spp
Coccidiodes immitis
Pathogenicity
Cause coccidiodomycosis ; a pulmonary disease
Desert soil, pottery, archaelogical middens,
cotton, and rodent are all harbour of C.immitis
Morphology and culture
On Sabouraud agar : grow as mold in 2 weeks
Barrel- shaped (smaller at the edges, wider at
middle) asexual spore
Specimen
Sputum,
Diagnosis
Complement-fixaton
Slide agglutination
Immunodiffusion
Prevention
Maintain general good health
Prevent other cause of damage to the immune system
Yeast-like of coccidioides
immitis
Tata.