Professional Documents
Culture Documents
1
Introduction
2
Introduction
3
Introduction
4+ 3+ 2+ 1+ (+) --
4
Blood grouping
L-Fucose
D-Galactose
H A B N-Acetylgalactosamine
N-Acetylgluosamine
5
ABO blood group
3% 45% 8% 44% 6
ABO blood group
6 tubes mandatory
RBC antigen
patient patient patient A B patient
Antibody
Anti-A Anti-B Anti-AB patient patient patient
7
ABO blood group
Interpretation
RBC
patient patient patient A B patient
Antibody
Anti-A Anti-B Anti-AB patient patient patient
8
ABO blood group
Phenotype
Genotype
Genotype
Phenotype
Children
9
ABO compatibility
O AB
10
ABO compatibility
11
ABO compatibility
O AB
12
ABO compatibility
O AB
13
Rh blood group
Homozygotes D/D
When D+ (familial studies)
Heterozygotes D/-
14
Rh blood group
épitope antigène
15
Rh blood grouping
Agglutination + Rh positive
No agglutination Rh probably negative
16
Rh compatibility
Rh negative Rh positive
17
Pre-transfusion cross-match
Objectives :
To verify ABO compatibility
To detect irregular antibodies in the recipient’s serum
against RBC to be transfused (previous transfusion or
pregnancy)
Principle :
Donor’s RBC + recipient’s serum, at least
In saline medium IgM antibodies
With antiglobulin IgG antibodies
18
Pre-transfusion cross-match
19
Pre-transfusion cross-match
Precautions :
Tf°
1. One antibody ------------ several antibodies
20
Consequences of an incompatible transfusion
Biological diagnosis :
Haemolysis : bilirubin, LDH,…
Identify the responsible antibody
21