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NUCLEIC ACIDS
Purines
Pyrimidines
Sugar
Nitrogenous bases
Ribose
Deoxyribose
Nucleosides
Phosphates
+nucleoside=nucleotide
The Sugars
The Bases
PYRIMIDINES
PURINES
Purines:
Pyrimidines:
RNA only
DNA only
Nucleotides and
Nucleosides
TheCis
named15
1
4
2
Nucleotide
Nucleoside
RNA
Resume
DNA
Nucleotides and
Nucleosides
BASE
NUCLEOSIDE
DEOXYNUCLEOSI
DE
2-deoxyadenosine
Adenine
Adenosine
Guanine
Guanosine
Cytosine
Cytodine
2deoxyguanosine
2-deoxycytodine
Uracil
Uridine
Thymine
Nucleotid
e
Analogs
as Drugs
Nucleic Acids
make up 13-34% of the dry weight in bacteria
deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)
Nucleotide: a building block
OH
HO
P
O
CH2
Base
H H C
C C
H
H or OH
Sugar:
RNA ribose (OH)
DNA deoxyribose (H)
Bases:
adenine (A), cytosine (C), guanine (G),
thymine (T)
RNA uses uracil (U) instead of thymine
DNA Stabilization
Complementary Base Pairing
DNA Stabilization-Base
Stacking
DNA Stabilization--H-bonding
between DNA base pair stacks
2.0 nm
10 bases/ turn
3.4 nm/ turn
G-C Content
Slime mold~22%
Mycobacterium~73%
CONSEQUENCES OF GC
CONTENT
GC slightly denser
FORMS OF DNA
Supercoiling
Cruciform
Structures
DNA is Dynamic
Denaturation of DNA
Denaturation by
heating.
How observed?
A260
For dsDNA,
A260=1.0 for 50 g/ml
For ssDNA and RNA
A260=1.0 for 38 g/ml
For ss oligos
A260=1.0 for 33 g/ml
Hyperchromic shift
Importance of Tm
Factors Affecting Tm
Renaturation
Temperature
Salt concentration
DNA concentration
Time
Cot Curves
Clustered
Dispersed
RNA
Types
mRNA
tRNA
rRNA
snRNA
siRNA
Ribozymes
Behavior in Acids
Behavior in Bases
Hydrolysis by Enzymes
Nucleasecatalyzes hydrolysis of
phosphodiester backbone
Exonucleases
Endonucleases
General. Ex DNAse I
Specific Ex. Restriction
endonucleases
Ribozymes
Restriction
Enzymes
RIBOZYME
S
Catalytic
RNA
Can work
alone or with
proteins
Therapeutic
applications?
SEQUENCING
Purposedetermine nucleotide
sequence of DNA
Two main methods
Uses
dideoxynucleotide
s (dideoxyadenine,
dideoxyguanine,
etc)
These are
molecules that
resemble normal
nucleotides but
lack the normal
-OH group.