Professional Documents
Culture Documents
fertilization
In vitro pollination
Pollination and fertilization under in vitro conditions hybrid embryos in plants that cannot cross by
conventional methods.
In nature, Intergeneric or Interspecific hybridization
occurs less frequently.
This is due to barriers hindering the growth of the
pollen tube on the stigma or style.
As early as 1926, Dahlgren reported fertilization and
seed development in Codonopsis ovata - in vitro
pollination after cutting off the style at its base.
Pre-zygotic barriers
Differences in floral
structure and inhibition
of pollen tube growth.
Post-zygotic barriers
Malformation
of
endosperm
and
the
inhibition of germination
In vitro
pollination
In vitro fertilization
In vitro fertilization involves three basic
microtechniques:
isolation, handling and selection of egg cell,
sperm cell and central cell
in vitro fusion of isolated sperm cell with egg
cell or central cell and
Culture of the in vitro formed zygotes and
primary endosperm cells (both single cells).
In-vitro fertilization
Essentialities
Time of anthesis
Time of dehiscence
Time of germination of pollen tubes into ovules
Viability of ovules
viability of pollen grains
receptivity of stigma to pollen grains
pollen germination and pollen tube growth
fertilization and embryo and endosperm
development
Applications
Overcoming self-incompatibility
Overcoming cross-incompatibility
Haploid
production
parthenogenesis
through