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CULTURE PLATES IN
BACTERIOLOGY
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inoculation,
important information
is written on the bottom
of the plates, close to
the rim
1date
of inoculation
2.temperature
3.duration
of incubation
of incubation
4.microorganism
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inoculated
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What is streaking
The
Streak
plates
1.With
2.A
sterile loop (flamed until red hot, then cooled by touching the
agar away from the inoculated sample) is then used to spread
the bacteria out in one direction from the initial site of
inoculation. This is done by moving the loop from side to side,
passing through the initial site
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What is streaking
The
.This
What
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are several
essential
precautions that
must be taken
during inoculation
procedures to
control the
opportunities for
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all
requirements
are within
immediate
reach and
must be
completed as
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must be
immediately
warmed by
flaming so that
any air movement
is outwards and
the vessel held as
near as possible
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Exposure to
Environment should
limited
to
minimum
During
manipulations
involving a Petri
dish, exposure of
the sterile inner
surfaces to
contamination
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parts of sterile
pipettes that will be
put into cultures or
sterile vessels must
not be touched or
allowed to come in
contact with other nonsterile surfaces, e.g.
clothing, the surface of
the working area, the
outside of test
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tubes/bottles
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Flaming procedure
The
flaming procedure is
designed to heat the end
of the loop gradually
because after use it will
contain culture, which
may splutter on rapid
heating with the
possibility of releasing
small particles of culture
and aerosol formation
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Handling of the
Inoculating loop
Position the
handle end of
the wire in the
light blue cone
of the flame.
This is the cool
area of the flame
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The
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Handling matters in
getting the right inoculum
Lift the bottle/test tube
containing the inoculum
with the left hand. .
Remove the cap/cotton
wool plug of the
bottle/test tube with the
little finger of the right
hand. .
Flame the neck of
the bottle/test tube.
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Replace the
Practice for Perfection
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cap/cotton wool
plug on the
bottle/test tube
using the little
finger. Place
bottle/test tube on
bench. . Partially
lift the lid of the
Petri dish
containing the
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Remove
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Remove
Complete the work
Close
the the
loop
and close the Petri
petri dish
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References
Basic
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Program
doctortvrao@gmail.com
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