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Microbiology
Branches of Microbiology
i) Bacteriology
ii) Virology
iii) Immunology
iv) Paracytology
v) Mycology
Study of bacteria is known as Microbiology
Structure of Bacteria
Essential Structure :
From inside to outside
i) Nucleus
ii) Cytoplasm
iii) Cytoplasmic membrane
iv) Cell wall
Cell Wall
Peptidoglycan : The strength of cell wall
Is chiefly provided by peptidogycan.
composed of repeating units of N-acityl
muramic acid and N-acityl glucosamine
with tetrapeptide side chain
Function
1. It prevents osmotic lysis of bacterial cell.
2. It gives shape of the cell.
3. It is associated with its own biosynthesis.
4. It is antigenic in nature.
5. It helps in cell division.
Spheroplasts :
Derived from gram negative bacteria and retains the
remnants of the other membrane.
Protoplasts :
Derived from gram positive bacteria that completely
lack cell wall.
Both one produced by under the influence of cell wall
inhibitors and one osmotically fragile Hypertonic
condition is necessary for their maintenance.
L-form :
Cell wall deficient forms of bacteria develops either
spontaneously or under the influence of cell wall
inhibitors.
May revert back to its normal form.
Cytoplasmic Membrane
Function :
Selective permeability and transport of
solutes.
Electron transport and oxidative
phosphorylation.
Excretion of hydrolytic enzyme.
Possess the enzymes and carrier
molecules.
Synthesis of structural components.
Mesosome
Convoluted, multi-laminated, membranous sac like
structure formed by invaginations of cytoplasmic
membrane. These serve as source of respiratory
enzyme.
Types of Mesosomes :
Septal mesosome
Latera; mesosome
The septal mesosome is attached to bacterial
chromosome and is involved in DNA segregation
and the formation of cross-wall during cell division.
Capsule :
It is an outer well - defined covering of
thick condensed, jelly like materials that
surrounds the bacterial cell wall.
It is composed of polysaccharide or
polypeptide.
Function :
i) It prevent phagocytes
ii) It enhances bacterial virulence, certain bacteria are
pathogenic only in capsulated state.
iii) It is antigenic in nature
iv) Specific identification of bacteria can be made by
using antiserum against the capsular
polysaccharide
All caps are polysaccharide except bacillus.
They are polypeptide
Capsulated bacteria:
Pneumococus
Klebsiella pneumonia
Bacillus anthrcis (polypeptide)
Glycocalyx
Flagella
Types :
i) Monotrichous Single polar flagellum,
eg.- vibrio cholerae.
ii) Amphitrichous Single flagellum attached
to each end eg. pseudomonas
iii) Lophotrichous Tufts of flagella at one
end eg. Spirillum minus.
iv) Peritrichous Numerous flagella all over
the bacterial body. eg. salmonella typhi
Fimbria
These are thin, short, straight appendages,
extruding from the cytoplasmic mambrane.
These are found in some gram negative
bacteria and are always arranged
peritrichously.
More numerous than flagella.
These are antigenic and associated with
virulence.
Types :
Common (Ordinary) pili
Sex pili
Function :
i) Adhesion: Pili are organs of adhesion.
ii) Sex pili : These are specialized, longer and
responsible for transfer of genetic factor in
bacterial conjugation.
Adhesion is the pre requisite for infection.
Y
R-factor
Resistant
Sensitive
Sex pilus
Resistant
Bacterial Spores
Spores are highly resistant, dormant stage
of bacteria formed in adverse
environmental conditions such as
starvation and desiccation.
Spores :
Endospore
Exospore
Structures of Spore:
Core
Spore cell
Cortex
Coat
Exosporium
Spore
Sporulation
Vegetative form
Germination :
The process of conversion of a spore into a
vegetative form. It consist of three stages
a) Activation by agents which damage the coat
such as heat, low pH, abrasion.
b) Initiation
c) Outgrowth
Plasmid
Plasmid
Are extra chromosomal double stranded circular
DNA molecules that are capable of replicating
independently of the bacterial chromosome
Plasmids carry the genes for following function
1. Drug resistance by R factors
2. Exotoxin including several enterotoxin
3. Metabolic activity.
4. Transfer of genetic material by sex pilli.
Transposons