Professional Documents
Culture Documents
Binary Division
1 to 2 to 4 to 8 to ?
Phases of Growth
Lag
Adapt to nutrients
Log
Active growth
Stationary
Death = Growth rate
Death
Nutrients consumed
pH too low (why?)
Log Growth
Measuring Growth
Direct Counts
Petroff-Hauser Chamber
Pros vs. Cons
Serial Dilution
10-fold serial dilutions
Pros vs. Cons
Measuring Growth-contd
Turbidity
Spectrophotometer
Scale
%Transmittance
Optical Density or Absorbance
Filtration
0.45 - 0.2 um sizes
Grid Pattern on Filter
Standards for Public Health
0 E.coli / 100 ml of water
Temperature
Psychro-, Meso- Thermophiles
Growth Curve
Problems
Temperature Problems
Thermophiles
Hot tubs-public
Heat Resistant Enzymes in PCR
Psychrophiles
Cold food (growth in transit)
Listeria grows in hot dogs
Salt Concentration
Halophiles
Great Salt Lake
Salt Pork
Oxygen
Aerboes vs. Anaerobes + ------ + ----------
Osmotic Pressure
Pseudomonas grows in distilled water
Jelly- Jams (high sugar)
Salt Pork (high salt)
Nutritional Factors
Carbon Source
Nitrogen Source
NO3- ==> NO2 ==> NH3 to Amino Acids ==>
Proteins
Sulfur
Phosphorus
Trace Elements
Fe, some required
Host Sideophores bind Fe
More -trophs
Auxotrophs
Can live on simple compounds
NO3-, Glucose
Make all Amino Acids, Vitamins, etc.
Prototrophs
Require complex compounds
Vitamins, Amino Acids
Fastidious
Most Pathogens
Types of Media
Complex Medium
Peptones, Yeast Extract
Fastidious
Prototrophs
Synthetic Medium
Minimal Media
Auxotrophs
Exoenzymes
Oxygen Requirments
Classification
Aerobic
Anaerobic
Falcultative Anaerobic
Microaerophilic
FTM Media
Growth Chambers
Candle Jars
Microaerophillic
Gas-Paks
Anaerobes & Faculatative Anaerobes