Professional Documents
Culture Documents
• Leukemias
Embryonic-fetal-adult
sites of hematopoiesis
• Yolk sac
• Mesonephros
• Liver*
• Spleen*
• Marrow
• Iliac crest
• Long bones
• Ribs
• Sternum
Sinusoidal marrow structure
Extravascular site of hematopoiesis, BMB
Blood cell exit
from marrow
100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
80 60 40 20 0
Maturation (nuclear area)
Hgb
RNA
Colony stimulating
factors
(GM-CSF, G-CSF)
• Inflammatory cytokine
• Inhibits apoptosis
• Enhance function of
mature cells
Granulopoiesis
• Nuclear shape change
• Nuclear condensation w/o pyknosis
• Cytoplasm: loss of basophilia, appearance then
loss of granules
• Loss of mitotic activity
Maturation
pool*
Proliferation
pool
Hematopoietic
Stem Cells
Engagement (HSCs) Totipotency
Self-renewal