In vivo determination of

the hypoglycemic effect of

Mollugo oppositofollia
Linn. (Molluginaceae)
plant extracts in Alloxaninduced male SpragueDawley rats
HERNANDEZ, RAY CONRAD. , JAVIER, JEZREEL ROLLAN., LIM, ALEC JACOB, MANUEL,
ROSELLE, PENSOTES, DARRYL VAN , RAMORAN, EDRICK GLENN
DR. GAVINO IVAN TANODRA

University of Santo Tomas

Faculty of Pharmacy

Introduction

Diabetes Mellitus
Disorder

in which blood sugar (glucose) levels

are abnormally high.

Metabolic

diseases characterized by
hyperglycemia

Defects in the B-cells of the pancreas

(American Diabetes Association, 2003)

Type 1 Diabetes
insulin-dependent
juvenile-onset
autoimmune

the pancreas

diabetes

diabetes

destruction of the -cells of

Insulin

hormone
controls

released from the -cells of pancreas

the amount of glucose in the blood

Prevalence of Diabetes
According

to the International Diabetes

Federation & World Health Organization
387

million people around the world have diabetes

cause

of 1.5 million deaths and is projected to rise

by more than 50% in the next 10 years

7th

leading cause of death in the world

Alloxan
2,4,5,6-

7
pyrimidinetetrone

diabetogenic

agent

induce

Type 1 diabetes in experimental

animals

administered

parenterally

intravenously

intraperitoneally

subcutaneously.

Usual

dose is 65 mg/kg

Mollugo oppositofollia Linn. :

Botanical Description
Kingdom: Plantae
Phylum: Tracheophyta
Class: Magnoliospida
Order: Caryophyllales
Family: Molluginaceae
Genus: Mollugo
Species: Mollugo oppositofolia

Mollugo oppositofollia Linn. :

Botanical Description

Characterized by diffuse prostrate, annual

herb

Made up of numerous, dichotomously

branched slender stems.

Possesses 13-22 mm long leaves, in

whorls of 4-5, unequal, and oblanceolate

Consists of small, white flowers, in

axillary fascicles of 2 or more, with long
filiform pedicels

Mollugo oppositofollia Linn.:

Botanical Description

10

applied

topically for the treatment of skin

diseases and itch

used

with castor oil as treatment for ear ache

natural

source of antioxidant

Mollugo oppositofollia Linn.

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Problem Rationale

Objectives
To

determine which fraction will elicit the

highest hypoglycemic activity.

To

identify the constituents present in

the different fractions.

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Scope and Limitations

Determination

of efficacy by decreasing blood

glucose level using a glucometer

Toxicity

will not be performed

Lingayen
Male

Sprague-dawley rats will be used

Diabetes
Soxhlet

induction by use of Alloxan

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Significance of the Study

To

serve as a tool for application of what the

researchers learned in the field of pharmacy.

To

serve as a reference for future

hypoglycemic studies.

To

provide information about the hypoglycemic

effect of Mollugo oppositifolia Linn.

Best?

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Research Design

Research Design

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Collection of Mollugo oppositifolia Linn. plant

sample
Identification of the wholeplant
sample
Drying of the plant sample

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Grinding of the plant sample

Extraction with hexane, chloroform,

2-butanol, methanol

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Rotary Evaporator
Soxhlet Apparatus

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IACUC
Application
Induction of diabetes to
male Sprague- Dawley rats

Phytochemical
Analysis

 Group

I: Normal healthy control animals

(fed with normal chow and water)

Group

II: Diabetes induced control

animals treated with alloxan
monohydrate in sterile saline (150
mg/kg by i.p. injection, single dose)

Group

III: Diabetes induced animal

treated with hexane extract (500
mg/kg/day orally for 35 days)

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 Group

IV: Diabetes induced animal

treated with chloroform extract (500
mg/kg/day orally for 35 days)

Group

V: Diabetes induced animal

treated with 2-butanol extract (500
mg/kg/day orally for 35 days)

Group

VI: Diabetes induced animal

treated with methanol extract (500
mg/kg/day orally for 35 days)

Group

VII: Drug control-animals,

diabetes induced rats treated with
Insulin (1 U/200 g /day intraperitonially

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Administration of crude
extracts
Biochemical Analysis

Statistical Analysis
Glucometer

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Thank You