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Workflow
Results
37 oC
Pseudomonas
aeruginosa
nd
nd
nd
nd
nd
II
bb
nd
nd
nd
nd
III
nd
nd
nd
nd
nd
Sample
Coliforms
Faecal
coliforms
Results
temperature
days
Results
Results
Results
Citrobacter freundii
days
Results
Conclusion
All bottles of natural mineral water was in accordance with microbiological safety
given in Regulations on the quality of quality and other requirements for natural
mineral water, natural spring water and table water.
It was confirmed that use of low-nutritious media, such as R2A gives us more
realistic results, when compared to high nutritious PCA, especially when it comes
to presence of saprophytic, autochthonous bacteria from the water.
Pathogenic bacteria tend to die off at room temperature more rapidly than at
refrigerator temperature, with the exception of P. aeruginosa, which even tend to
multiply rapidly at room temperature.
When stored in refrigerator P. aeruginosa kept its initial number during period of
75 days, while other pathogens tend to decrease in number.
Dr Dragoljub Cvetkovi
Dr Aleksandra Velianski
Dr Sinia Markov