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INFLUENCE OF STORAGE CONDITIONS ON

SURVIVAL OF SELECTED BACTERIA IN


BOTTLED MINERAL WATER

Blue Planet Earth

Blue Planet Earth


1,386,000,000 cubic km (332,500,000
cubic miles)
ALL WATER on our planet: oceans, seas, ice
caps, lakes and rivers, groundwater,
atmospheric water, even water in you, your
plants and pets.
2.5% of total volume is FRESH-WATER
(that tiny sphere), of which:
68.9% glaciers and permanent snow
30.8% groundwater (soil moisture, swamp
water permafrost )
0.3% Lakes and Rivers (that tiny dot)

Pack it Sell it
What ARE you paying for?

Current regulations in Serbia:


NO coliforms, faecal coliforms, faecal enterococci nor Pseudomonas aeruginosa in
250 mL
Total count 10* CFU/mL

The Aim of Our Study


How does different storage conditions (temperature, light) effect the bacteria
present in bottled natural mineral water CONTROL sample
How does the number of pathogens change in artificially contaminated water
under the same storage conditions

Workflow

Materials and Method


Microorganisms for artificial contamination Citrobacter freundii, Escherichia
coli, Enerococcus faecium, Pseudomonas aeruginosa (DensyCheck, pour plate)
Control sample total count bacteria in original packages
Media for total count:
PCA
R2A (American Public Health Association, APHA)
Membrane filtration:
Citrobacter freundii, Escherichia coli - Chromocult Coliform Agar (CCA)
Enerococcus faecium - Chromocult Enterococci Agar (CEA)
Pseudomonas aeruginosa - Cetrimid Agar (CA)

Results

Preliminary testing of different brands of bottled natural mineral water

Total count (CFU/mL)


22 oC

37 oC

Pseudomonas
aeruginosa

nd

nd

nd

nd

nd

II

bb

nd

nd

nd

nd

III

nd

nd

nd

nd

nd

Sample

Coliforms

Faecal
coliforms

Results

Temperature profile in room during storage period

temperature

days

Results

Results

Results
Citrobacter freundii

days

Results

Conclusion
All bottles of natural mineral water was in accordance with microbiological safety
given in Regulations on the quality of quality and other requirements for natural
mineral water, natural spring water and table water.
It was confirmed that use of low-nutritious media, such as R2A gives us more
realistic results, when compared to high nutritious PCA, especially when it comes
to presence of saprophytic, autochthonous bacteria from the water.
Pathogenic bacteria tend to die off at room temperature more rapidly than at
refrigerator temperature, with the exception of P. aeruginosa, which even tend to
multiply rapidly at room temperature.
When stored in refrigerator P. aeruginosa kept its initial number during period of
75 days, while other pathogens tend to decrease in number.

THANK YOU FOR YOUR ATTENTION

MSc Marina Raji


rajich.marina@gmail.com

Dr Dragoljub Cvetkovi

Dr Aleksandra Velianski

Dr Sinia Markov