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Mystery Solution

Challenge
Which solution contains:
Glucose
Starch
Protein?
• Mr Stuart has mixed
up 5 important
food samples (S1-
S5) and needs to
find out which is
which
• You are the food
detectives that can
use science to help
him
These are Mr Stuart’s
Samples
• 2% Egg Albumen
• 20% Glucose
• 1% Starch
• 0.2% Glucose
• 0.1% Starch

• But which is which???
In front of you there are the 5
samples
• Take a small amount of one sample and
place in to 3 test tubes so they fill to
1/3 full
• You need to do all three tests to confirm
which solution is which

• Complete the first test to decide if it
contains Starch
• Then the second to test for Glucose
• The third to determine Protein
presence
Test for starch
• Put a few drops of the mixture from
jar 1 in a test tube. Add a drop of
iodine to the tube.
• A positive result turns dark blue 

Test for sugar
(glucose)

• A couple of drops of Benedict’s soln


is added to a third of a test tube of
the sample
• Place the sample into a water bath
and leave for 5 mins
• Whilst waiting set up the next test

• A positive result turns orange/ brick
red
A Biuret test can be performed
in the laboratory to identify the
presence of peptide bonds
• The tube on the left shows a
negative result. 
• The tube on the right is a positive
test for protein.  Note the purple
colour of the positive test.
Results
Test Tube Contents
S1
Sample
S2
S3
S4
S5

• 2 % E g g A lb u m e n
• 20 % G lu co se
• 1 % S ta rch
• 0 .2 % G lu co se
• 0 .1 % S ta rch
Results
Test Tube Contents
S1
Sample Starch
S2 Starch
S3 Glucose
S4 Glucose
S5 Protein

• 2 % E g g A lb u m e n
• 20 % G lu co se
• 1 % S ta rch
• 0 .2 % G lu co se
• 0 .1 % S ta rch
• http://images.google.co.uk/imgres?imgurl=
To make a vit-C indicator
solution Experiment 1-
• Mix 1 tablespoon of cornstarch into enough water to make a paste.
• To this paste, add exactly 250 millilitres of water and boil for five
minutes. Allow to cool.
• Add 10 drops of the starch solution to 75 millilitres of water by
using the eyedropper.
• Add enough iodine to produce a dark purple-blue color. Now the
indicator solution is ready.
• Put 5 millilitres of indicator solution (roughly 1 teaspoon) in a 15-
milliliter test tube (one for each sample)
• To the test tube, use a clean eyedropper to add 10 drops of juice
from teh fresh fruits. For solids, pulp them in a blender and
strain the juice well. Washand clean the eyedropper for each
sample. Shake well.
• Place all of the test tubes against a white background.
• Line up the tubes from lightest to darkest purple. The lighter the
solution, the higher the level of Vitamin C content. That's
because Vitamin C causes the purple indicator solution to lose
its color.

Experiment 2-
• Repeat the whole process of
Experiment 1.
• Repeat the whole process of
Experiment 1, this time with the
concentrate juices, not the pure
ones.
• Compare the amount of Vitamin C of
the concentrate juices and the
fresh pure ones
Experiment 3-
• Do the whole process of Experiment
1, but use Kool-aid, Sunny Delight,
and, C-plus instead of the fruit
juices.
• Compare the results from these
juices with the juices with the juices
from concentrate and the fresh
pure ones.

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