Visual Presentation at the COMCEDS Nationals '14 Poster Exhibition, December 17th, 2014, Lahore,

Pakistan

Production of Ethanol from Potato Waste using Acremonium

Cellulolyticus under Very High Gravity Conditions
Micaiah Cyril Das, Noor-ul-Ain., Sundus Khushnood, Saeed Gul
Department of Chemical Engineering, University of Engineering & Technology Peshawar, Pakistan

________________________________________________________________
micaiahcyrildas@hotmail.com , saeed.gul@uetpeshawar.edu.pk

Issues

Table 1: Max. Ethanol Yield for different Nitrogen Sources . [3]

Kinetic Parameters
YE
PM
HF

Increase in fuel consumption.

Fossil fuel Depletion.
Economics.

Max. Ethanol Produced (gram per

liter)
Max. Glucose Consumed (gram per
liter)
Growth Rate (cfu/ml/h)
Production Rate (gram per liter per
hour)
Consumption Rate (gram per liter per
hour)
Yield (product/subs)*100%

Solution
Renewable Energy resources
Bio-Energy to be specific.
Bio-ethanol, gas and diesel are
some of the bio-fuels that can
be used.

FM

MBM

30.8

35

24.59

32.9

25.54

70

73.9

53.2

68.8

53.2

0.28
3.68

0.21
3.2

0.19
1.97

0.28
3.59

0.13
2.36

-7.07

-5.35

-4.32

-5.06

-7.25

43.8% 47.3% 46.2% 47.8%

48%

Why bio-ethanol?
Worldwide production of ethanol has escalated since the last
5-10 years[1].
25000.00
20000.00
15000.00
Billion Gallons

10000.00
5000.00
0.00

2007
USA
China

2008
Brazil
Canada

2009

2010

2011

2012

2013

Europe
Rest of World

Figure 1: Global Ethanol Production by Country/Region and Year. [1]

Figure 3: Ethanol conc. with time, during fermentation of VHG potato mash. [4]

Project Overview
Figure 4: Proposed Process Flow Sheet

This Project will demonstrate effect of,

Conclusion:

Using SSF Process

Use of A. Cellulolyticus as a single enzyme source
Employing Very High Gravity (VHG) conditions
Testing various nitrogen alternatives to yeast extract,
on Ethanol yield
30

Literature
25
20
15 (g/L)
Glucose
10
5
0

Comercial

Trihcoderma

A.Cellulolyticus

Figure 2:Comparison of Glucose yield From Different Enzymes. [2]

It has been observed that higher rates, yields and concentration of ethanol
are possible for SSF than SHF at lower enzyme loadings. Also it is
possible to achieve high ethanol production using only a single
microorganism and even a further yield if nitrogen source is changed. An
estimate yield of 92% can be achieved.

References
[1] F.O. Licht, cited in Renewable Fuels Association, Ethanol Industry Outlook 20082013 reports. Available at www.ethanolrfa.org/pages/annual-industry-outlook
[2] Min-Tian Gao et al., Production of ethanol from potato pulp: Investigation of the
role of the enzyme from Acremonium cellulolyticus in conversion of potato pulp
into ethanol, Process Biochemistry, 2012, 47, 21102115.
[3] Gulten Izmirlioglu et al. , Ethanol Production from Waste Potato Mash by Using
Saccharomyces Cerevisiae, Applied Sciences, 2012, 2, 738-753.
[4] Sathaporn Srichuwong et al. , Simultaneous Saccharification and fermentation
(SSF) of very high gravity (VHG) potato mash for the production of ethanol,
biomass and bioenergy 2009, 33, 890898.