Professional Documents
Culture Documents
Energy Generation
An
Overview of Metabolism
Stage
Definitions
Oxidation
Reduction
Redox reactions
Standard Reduction potential
Oxidative phosphorylation
Chemiosmosis
Electron transport chain
Oxidation
Reduction
Redox reactions
= Redox couple
Oxidative phosphorylation
Chemiosmosis
Electron
Transport and
Oxidative Phosphorylation
theory
Oxidative
Phosphorylation
Some of the energy liberated during
electron transport is used to drive the
synthesis of ATP in a process called
oxidative phosphorylation
The
chemiosmotic hypothesis of
oxidative phosphorylation (Peter
Mitchell)
Membrane-bound
carriers transfer
electrons to oxygen across a chain
Cytochromes,
Postulates
Blockers
inhibit
This
Net result:
1
Uncouplers
allow electron flow, but disconnect it from oxidative
phosphorylation (inhibit ATP synthesis, not electron
transport)
Uncouple electron transport from Ox-Phos The
energy from electron transport is lost as heat, not ATP
Pasteur Effect
Respiration:
Fermentation
Example of fermentation:
Glycolysis:
Glucose Pyruvate Lactic Acid or Ethanol
The final electron acceptor = pyruvate (or a product of
pyruvate such as acetaldehyde intermediate)
The
Breakdown of Glucose
to Pyruvate
The
glycolytic (Embden-Meyerhof)
pathway is the most common pathway
and is divided into two parts:
The
ATP
The
1 Glucose 2 Pyruvate
2 ATP used
4 ATP generated
Net yield = 2 ATP
2 NAD used
2 NADH generated
Net yield = 2 NADH (THIS MUST BE OXIDIZED TO
KEEP THIS PATHWAY RUNNING.)
The
The
Fermentation
In
Homoloactic acid
Alcoholoic
Propionic acid
Butylene glycol (Butanediol)
Mixed acid fermentation
Butyric acid, butanol, acetone
#1:
Pyruvate
#2:
lactic acid
Alcoholic fermentation
Pyruvate
acetaldehyde ethanol
Pyruvate
Pyruvate
Voges-Proskauer Test
CO2/H2 ratios
Mixed acid ~1:1
Butanediol ~5:1
Respiration
Aerobic Respiration
Anaerobic Respiration
Escherichia
Enterobacter
Bacillus
Pseudomonas
Micrococcus
Rhizobium
TEA = sulfate
Desulfovibrio
Desulfotomaculum
The
Tricarboxylic Acid
Cycle-a series of reactions
Acetyl-CoA
(produced by
decarboxylation of pyruvate) reacts
with oxaloacetate to produce a 6carbon molecule
Subsequently, two molecules of carbon
dioxide are released, regenerating the
oxaloacetate
ATP is produced by substrate-level
phosphorylation
Catabolism
of Carbohydrates
and Intracellular Reserve
Polymers
proceeds
by either hydrolysis or
phosphorolysis to produce molecules
that can enter the common catabolic
pathways already discussed
Lipid
Catabolism
Lipases
Degrade
acids
Fatty
Protein
Proteins
Anabolism
Polysaccharide biosynthesis
Capsular polysaccharides
Outer membrane LPS
Glycogen
PTG (NAG:UDP intermediate)
Lipid biosynthesis
Autotrophs
Majority of prototrophs
Similarly do not require added amino acids
Some bacteria (e.g. Neisseria and Streptococci) require
preformed amino acids
Auxotrophs