PKPPT Midbit Eng

For internal use only!
Eppendorf In touch with life
BioFlo and CelliGen 510 Series

Joe Capone Enfield CT, March 15th, 2013





Adaptability
BioFlo/CelliGen 510
Cell Culture Impellers > Agitation speed slower than in Fermentation process > Impeller designed to have more streamlined blades to prevent excess turbidity and minimize bubble breakage > Each Impeller designed for specific culture application
Left to right: Spin Filter, Cell Lift with Screen and Foam Elimination Chamber, Pitched Blade, Marine Blade
Image description: Impeller Diversity.
Eppendorf AG | BioFlo and CelliGen 510 Series | Joe Capone | March 15, 2013
Adaptability
BioFlo/CelliGen 510
Pitched and Marine Blade Impellers > Commonly used in cell culture applications > Pitched Blade and Marine Blade impeller are commonly used in applications that require gently mixing with low shear > Impeller design provides both axial and radial mixing throughout the reactor for uniform mixing of contents
Image description: Pitched and Marine Blade Impellers
Marine Blade
Pitched Blade
Adaptability
BioFlo/CelliGen 510
Spin Filter Impeller > Shaft and sanitary compression style marine impeller > Used in batch, Batch-Fed, and perfusion processes > 10 Suspension Culture or 75 Microcarriers
Image description: Spin FIlter Impeller
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Adaptability
BioFlo/CelliGen 510
Spin Filter Impeller, continued > Medium passes through screen and Cells are retained outside > Dip Tube inside cage enables medium exchange > Mounted on same shaft as impeller or different shaft
Image description: Spin Filter Impeller
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Adaptability
BioFlo/CelliGen 510
Cell Lift Impeller > Used in batch, Batch-Fed, and perfusion processes > Impeller rotation creates negative pressure in the hollow impeller tube causing medium to circulate uniformly in a closed loop > Provides very low shear environment with high nutrient transfer and mixing
Image description: Cell Lift Impeller
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Adaptability
BioFlo/CelliGen 510
Cell Lift Impeller, continued > Ring sparger is located inside the lower part of the impeller aeration cage providing indirect aeration > Medium passes the screen, cells and microcarriers stay outside > Air wash mitigates potential foaming
Foam elimination cage Screen EFTE or stainless steel
Air Wash
Impeller Port Ring Sparger

Aeration Cage
Image description: Cell Lift Impeller
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Adaptability
BioFlo/CelliGen 510
Basket Impeller > Anchorage Dependent or Suspension Cells > 316 Stainless Steel > Requires Fibra-Cel Disks > Low pressure drop across the bed
> High Surface-to-Volume Ratio

> High mass transfer of nutrients and oxygen > Cells are less susceptible to shear forces from impeller blades and sparge gas bubbles > Great for perfusion process
Image description: Basket Impeller
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Adaptability
BioFlo/CelliGen 510
Automatic Pressure Control > For process required to be run at a specific pressure > Want to monitor pressure in SCADA for batch record purposes > Includes Pressure transmitter that sits on the headplate of the vessel > Includes Pressure Control valve on the exhaust line
Image description: Automatic Pressure Control.
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Adaptability
BioFlo/CelliGen 510
Exhaust Condenser > Condenser reduces evaporation and fouling of exhaust filter > Heating band on top heats liquid past dew point on top > Condenser will mitigate volume loss over the duration of a batch > Possesses a supply and return line for cooling
Image description: Exhaust Condenser.
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Adaptability
BioFlo/CelliGen 510
Gas Mixing > Two gas Fermentor (standard) > Oxygen and Air mixture for O2 Enrichment > Three gas Fermentor and Bioreactor (optional) > Oxygen, Air, and CO2 for pH control > Four gas option Bioreactor (standard) > Oxygen, Air, CO2, and N2 for DO control > System can be configured with Automatic mixing via TMFC or manual control with a rotameter > 4 TMFC can be added for individual gas control
Image description: Gas Mixing.
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Adaptability
BioFlo/CelliGen 510
Fermentation Overlay > Valve that allows gas to be delivered into the head space through a port in the upper side wall rather than through the traditional sparger mounted through the lower sidewall of the vessel > Ideal for anaerobic cultures that require an overlay with an inert gas
Image description: Fermentation Overlay.
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Adaptability
BioFlo/CelliGen 510
Airwash/Overlay System (Cell Culture) > Independent gassing system that consists of a 4 gas mixing solenoid valve bank, TMFC and filter housing with 0.22 filter > Single or a mixture of gases to be delivered into the head space through a port in the upper side wall > Ideal to introduce various gases into the headspace of the vessel as required by the process > Air Wash system is similar to overlay option above but has an additional barbed elbow inside the vessel to direct air onto the foam cage assembly on the cell lift impeller
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Adaptability
BioFlo/CelliGen 510
pH/D0/Redox Probes > Sanitary Device Ingold Port Adapter, Probe Housing, Probe and Cable > Lower side wall of the vessel has four (4) ports that can be used to insert probes > Redundant pH and DO transmitter and probes can be are installed and can be switched to primary if needed > This redundant probes offer noninvasive solution when customers do not want the retractable probe housings
Image description: Ingold Housing Schematic.
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Adaptability
BioFlo/CelliGen 510
Temperature Control Alternate > Two Heat Exchangers (option) > Super cool the vessel temperature control to less than 10 C > Faster temperature shifts for process needs > Water Conservation > Mitigates Environmental concerns
Image description: BioFlo/CelliGen 510 Heat Exchanger.
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Adaptability
BioFlo/CelliGen 510
QMI Safe Septum > Less expensive when compared to resterilizable valves > Can be used in an Emergency to add reagents to the culture quickly > Valve is sterile connection > Ability to pump in additions at a higher flow rate
Image description: QMI Safe Septum.
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Adaptability
BioFlo/CelliGen 510
Foam and Level Sensors > Two probes with different lengths; they are inserted with compression style sanitary design mounts into PG13.5 ports in the headplate of the vessel > Ability to link to a pump to add antifoam; they can also be used as level probes to add media or remove supernatant > Probes can be used as a high foam or level alarm
Image description: Foam and Level Sensors.
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Adaptability
BioFlo/CelliGen 510
Spray Ball Option > Used with a CIP Skid to facilitate automated cleaning of the vessels interior > To validate a Cleaning-In Place cycle
Image description: CIP Spray Ball.
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Adaptability
BioFlo/CelliGen 510
Baffle Cage > Four removable electropolished 316 L stainless steel baffles mount into the vessel head plate > BioFlo Fermentor Standard > CelliGen Bioreactor Optional
Image description: BioFlo/CelliGen 510 vessel Baffle Cage.
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Adaptability
BioFlo/CelliGen 510
Variable Speed Peristaltic Pump > Modified to add liquids via I/0 Module and added as a loop > Watson Marlow > Peristaltic > Various models (120U shown)
Image description: Variable Speed Peristaltic Pump.
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Adaptability
BioFlo/CelliGen 510
Stainless Steel Addition Cans > Sizes of the addition vessels offered are 4 L, 7 L, 11 L, 19 L, 38 L > Stainless Steel & Electropolished > Autoclavable > Comes with vent filter > Comes with a valve and tubing to connect to the addition valve
Image description: Addition Cans.
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Adaptability
BioFlo/CelliGen 510
Glass Addition Bottles > 0.25 L, 0.5 L, 1 L, 2 L, 5 L, 10 L > Supplied with a one dip tube bottle for aerobic cultures > Two dip tube bottle for anaerobic cultures; > Second dip tube is used to bulb and inert gas into the supplement being added > Glass Bottle with Stainless Steel Dip Tubes > Cap Assembly with Vent Filter > Autoclavable
Image description: Glass Addition Bottles.
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Adaptability
BioFlo/CelliGen 510
Sterile Sampling Assembly > Sterile sampling assembly is designed to be used in conjunction with systems sample valve > The bottle assembly is autoclaved; then the attached process valve is steamed onto the sample valve as shown prior to collecting the sample
Image description: Sampling Assembly Schematic.
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Adaptability
BioFlo/CelliGen 510
Utility Kits > Process Steam- Regulator only (assume steam is clean already) Water Kits > Main Steam- Filter & Regulator > Water- Filter & Regulator > Instrument Air- Filter & Regulator > Process Air- Filter & Regulator Instrument Air Kit
Image description: Water and Instrument Air Kits.
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Adaptability
BioFlo/CelliGen 510
Utility Kits Continued > Utilities MUST be at a specific pressure for system to operate properly > Utilities MUST be clean so as to not damage Main (Utility) Steam the equipment or culture Kits > Applications using Main Steam in place of Clean Steam > Fermentation- Depends on Process > Cell Culture- No Process (Clean) Steam Kits
Image description: Main (Utility) and Process (Clean) Steam Kits.
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Adaptability
BioFlo/CelliGen 510
Basic Validation Package > System Identification and Utility Requirements > List of Materials and Lubricants in Product Contact > Operational Verification of Process Control Loops, Switches, Indicators and Alarms > Equipment and filter list for all items on the P&ID Instrumentation List > Spare Parts List > General P&IDs > Operating manual, Loop diagrams, and Electrical schematics > Pressure Hold Verification and Media Hold Verification
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Adaptability
BioFlo/CelliGen 510
Basic Plus Validation Package > All of Basic ValidationPlus > Expanded Spare Parts List Documentation (includes specifications for each purchased part) > Enhanced Material Certification of Parts in Product Contact > Enhanced Pressure Vessel Documentation > Calibration of Process Measurements
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Adaptability
BioFlo/CelliGen 510
Enhanced Validation Package > All of Basic Plus ValidationPlus > Weld mapping of the process lines with videotape > Vessel Temperature Uniformity Verification (SIP) > Sterilization Verification with biological indicators
> CIP Coverage Test is additional validation test where Riboflavin is removed with DI water to confirm the spray ball coverage inside the vessel
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Eppendorf Support
Eppendorf Application Hotline > Phone: + 49 1803-666 789 (Hamburg-based Application Hotline, Germany) > E-mail: support@eppendorf.com (Europe, International) support_na@eppendorf.com (Eppendorf North America) Homepage: www.eppendorf.com
> Manuals
> Applications > FAQs
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Eppendorf Support
Eppendorf is a registered trademark of Eppendorf AG, Hamburg, Germany. New Brunswick is a trademark of Eppendorf AG, Hamburg, Germany. CelliGen, BioFlo, and BioCommand are registered trademarks of New Brunswick Scientific. Fibra-Cel is a registered trademark owned by Celite Corporation and licensed to New Brunswick Scientific Co., Inc., USA. Allen-Bradley is a registered trademark of Rockwell Automation, Inc., Milwaukee, Wisconsin, USA. NA-CONNECT is a registered trademark of Millipore, Nodinge, Sweden. Ingold is a registered trademark of Mettler-Toledo AG Corp., Greifensee, Switzerland. ProSoft is a registered trademark of Prosoft Technology, Bakersfield, California, USA. All rights reserved, including graphics and photos. Copyright 2013 by Eppendorf AG.
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PKPPT Midbit Eng

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For internal use only!

Eppendorf In touch with life

BioFlo and CelliGen 510 Series

BioFlo and CelliGen 510 Series

BioFlo and CelliGen 510 Series

BioFlo and CelliGen 510 Series

BioFlo and CelliGen 510 Series

BioFlo and CelliGen 510 Series

Image description: Impeller Diversity.

For internal use only!

For internal use only!

Image description: Spin FIlter Impeller

For internal use only!

Image description: Spin Filter Impeller

For internal use only!

Image description: Cell Lift Impeller

For internal use only!

Foam elimination cage Screen EFTE or stainless steel

Impeller Port Ring Sparger

Image description: Cell Lift Impeller

For internal use only!

> High Surface-to-Volume Ratio

For internal use only!

Image description: Automatic Pressure Control.

For internal use only!

Image description: Exhaust Condenser.

For internal use only!

For internal use only!

Image description: Fermentation Overlay.

For internal use only!

For internal use only!

For internal use only!

Image description: BioFlo/CelliGen 510 Heat Exchanger.

For internal use only!

Image description: QMI Safe Septum.

For internal use only!

For internal use only!

Image description: CIP Spray Ball.

For internal use only!

Image description: BioFlo/CelliGen 510 vessel Baffle Cage.

For internal use only!

Image description: Variable Speed Peristaltic Pump.

For internal use only!

Image description: Addition Cans.

For internal use only!

For internal use only!

Image description: Sampling Assembly Schematic.

For internal use only!

Image description: Water and Instrument Air Kits.

For internal use only!

For internal use only!

For internal use only!

For internal use only!

For internal use only!

For internal use only!

For internal use only!

BioFlo and CelliGen 510 Series

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