Water structures carrying DNA information, Application to HIV/AIDS and Autism

L.Montagnier, Borovets, October 23, 2013

The Evolution of Medicine Toward 4 P Qualities

P1 Preventive P2 Predictive P3 Personalized P4 Participative

Emergence or reemergence of new epidemics due to :

Globalization of exchanges and travels Demography: concentration in large cities Nutrition (pesticides, water) Environmental factors Climate changes, electromagnetic radiations Contacts with wild and farm animals Decline of immune defenses

The most important burden Chronic Diseases

- Cancers - Cardiovascular - Neurodegenerative - Arthritic - Autoimmune - Multifactorial, but in common: oxidative stress infectious agents (?)

Various environmental factors effects accumulate

Radiations (, X, UV, visible, hertzian) Air chemical pollution Inadequate Food Excessive physical exercize tobacco smoke alcohol ischemia Parasitic infections Bacterial infections Viral infections

+ + + + + + + + +

= DISEASE

Pathological effects of Oxydative Stress

Lipid hyperoxydation (plasma, cell membranes) Protein Oxydation --> destruction, agregates DNA oxydation --> Mutations, Chromosomal Breakage

Oxidative stress Weakens the immune system Activates transcription factors (NF-kappa B) Activates genes involved in cell division, inflammatory cytokines, lymphocytes activation Immune dysfunction, apoptosis (TH1 TH2)

The Human Genome

Genes (+ introns +promoters) Retroelements: retroviruses retrotransposons LTR Total Repeats 3% 8% 13% 23% 47% 50%

LINE SINE
Unknown

22% 13%
?

Our microbial ecosystem :

Mucosa
Constant exposure to microbial agents and immune protection

Skin

Extreme genetic plasticity of microorganisms:

virus : HIV, Influenza Bacteria (plasmids) Parasites (retrotransposons) against reaction of the immune system

How bacteria have learned how to persist despite the immune system
- Biofilms - Mobile antibiotic resistant genes - Nanoforms - Nanostructures - Endosymbiontes

Parasite
(Worm)
Filarial nematode

Bacterium
intracellular
Wolbachia

Virus

Persistent cold infections

Inability of the immune system to eradicate them (tolerance, oxidative stress) Non-multiplicative forms of classical bacteria intracellular bacteria Sanctuaries (bone marrow, joints, intestine, brain, .) Vectors (Parasites) Remote effects (toxin, nanostructures)
Antibiotics affect them only when they go out of sanctuaries

EXPLORING THE ROLE OF LATENT INFECTION IN CHRONIC DISEASES

A physical and molecular approach

Two technologies for detecting bacteria and viral DNAs

A new technology based on the production of electromagnetic waves The Polymerase Chain Reaction (PCR) using the 16S ribosomal DNA variability

Classical model of PCR

oligo
Taq polymerase

oligo

A newly discovered property of DNA :

Resonance emission of low frequency electromagnetic waves by high water dilutions of DNA.

Molecular recognition without close contact

(by waves and resonance)

Capture of the signals

Sample X 500 Signal Analysis software

Sensor coil

Amplifier

Computer

FACTS
Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of microorganisms (virus, bacteria) or from the plasma of humans infected with the same agents.

7-100 Hz

Filtrati on 0.1
0.02 2ng/1ml
16

10-2 10-3 10-4

10-5 10-6 10-7 10-8 10-9 10-10 10-12 10-13 10-14 10-15 10-

1000 3000 Hz

Amplitude

Noise

(+)

Time (sec)

Spectral Frequency Analysis

Fourier Tranformation

A positive signal is defined by: amplitude increase Shift to higher frequencies (500-2000 Hertz)

Noise

(+)

Frequency (1-20000 Hertz)

Suspensions of pure culture of bacteria with pathogenic potential are producing electromagnetic signals (EMS) in the range of dilution usually 10-8 10-13, sometime (E.coli) up to 10-18 They are: E.coli, Streptococcus, Salmonella, Staphylococcus, B.subtilis, Clostridium, Pseudomonas, etc.

Micro-organisms involved in EMS induction

1.DNA from main pathogenic bacteria

Streptococcus Staphylococcus Pseudomonas Mycoplasma pirum Salmonella Clostridium Proteus mirabilis B. Subtilis Borrelia burgdorferi Sutterella
- From viruses

HIV1 Influenza group A HBV HCV

M.pirum adhesin HIV genes

- Genes involved

Interpretation 10-2 10-9

DNA

Water excitated state crystal like gel no EMS

Free polymer self maintained by EMS

I DNAs emit EMS

II EMS are produced by water nanostructures (naneons) III EMS are producing naneons IV Naneons and EMS carry specific DNA information

EMS are produced by water nanostructures (naneons)

Evidence :
from filtration Size : between 20 and 100 nM for bacterial sequences Smaller that 20M for viral sequences from biophysical studies indicating spectrometral changes in the dilutions producing EMS

Naneons and EMS carry

specific DNA information
Natural and digital transmission

Classical model of PCR

oligo
Taq polymerase

oligo

PCR on water nanostructures

oligo

Taq EMS

oligo

DNA

Water Naneons

EMS

EMS
Water Naneons 7 Hz
PCR

DNA

Generator 7Hz
DNA -6 -6 water Tube 1 Tube 2 metal
-2 -3 -4 -5 -6

18hrs
water

+ + +
EMS

D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

Transmission in water of D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

DW: Distilled Water / FD2: Dilution 10-2 after filtration 450and 20 nM

Water-mediated photonic transmission of DNA

DNA

Water Naneons

EMS

Computer Digitized

Receiver

EMS
Computer

Analog

PCR

DNA

Water Naneons

Reproduction of DNA transduction in other laboratories

File EMS of 194 bp DNA from HIV1 LTR Sent to Benevento University, Molecular Biology Laboratory DNA reproduced and sequenced 100 % identical to original
File EMS of 499 bp DNA from Borrelia burgdorferi Sent to Laboratory of Chronix Biomedicals University of Gottingen

Molecular Biology Laboratory DNA reproduced and sequenced 100 % identical to original File EMS of 499 bp DNA from Borrelia burgdorferi Sent to Laboratory of Chronix Biomedicals University of Gottingen

Water-mediated photonic transmission of DNA

Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi) E.Schutz et al. Goettingen, 2011

ELF DNA WATER

(plasma)

DNA DNA DNA DNA

How pathogenic information can persist, and escape immune defence and treatment.

Two main problems for AIDS research in 2013

- The viral DNA reservoir
- The Higher sensitivity of African people to HIV infection

The dual origin of EMS in HIV/AIDS EMS

HIV < 20 nM

plasma RBC
++ +/++

Agent X < 100 nM

Evidence that the agent X is a bacterium

- Presence of a sequence close to 16 S ribosomal DNA of Rickettsiales - Sensitive to antibiotics ( in vitro and in vivo) - Growth in cell line - Electron microscopy - Associated with Red Blood Cells

Human sequences associated with the agent X 237 bp (human chromosome 1) 213 bp (human chromosome 7) and more

Strong correlation with HIV infection

- Contains human DNA sequences emitting EMS only in pathogenic HIV infection

-Sequence 213 bp from Chromosome 7

EMS in HIV + patients no EMS in HIV - patients

DNA
PCR HIV plasma RBC Risk factor 2 RBC Risk factor 1 RBC EMS

+
(LTR 195 bp) (all HIV)

(20 nM)

(100 nM) (213/237 bp) ( all HIV+, a few HIV-) (HIV+)

( all HIV+, many HIV-)(HIV- & HIV+) (100 nM)

RISK FACTORS OF HIV INFECTION

CONCLUSION 1
A new intracellular bacterium has been found in human red blood cells. A variant bearing extra DNA sequences is present in all HIV infected patients and also in some healthy HIV negative African individuals.

CONCLUSION 2
Origin of EMS :

not necessarily depending on DNA sequence

- depending on transmissible modification of DNA (free radicals of water ?)
- Association with pathogenicity

AUTISM :
THE GUT, BLOOD and BRAIN MICROBIAL CONNECTION

GUT

BLOOD

BRAIN

% EMS/noise

Correlation between EMS, antibiotic treatment and clinical signs in an autistic child

40 Accept various food More present with Family members

AZITHROMYCIN 30 -

CEFUROXIM
Starts speaking

20 -

10 -

0-

27

50

100

133

days

Environment al factors genetic susceptibility

Oxidative stress immunosuppres Bacterial sion agents Oxidative Somatic stress mutations prion effect
Reversible Less and less reversible

In press

Electromagnetic detection of HIV DNA in the blood of AIDS patients treated by antiretroviral therapy.
Luc MONTAGNIER^, Jamal AISSA, Claude LAVALLEE, Mireille MBAMY, Joseph VARON#, and Henri CHENAL

World Foundation for AIDS research and Prevention (UNESCO), 1 rue Miollis, 75015, Paris, France Nanectis Biotechnologies, France CIRBA Centre Intgr de Recherches Biocliniques dAbidjan, Ivory Coast # The University of Texas Health Science Center, Houston, USA.

Abstract : Electromagnetic signals of low frequency have been shown to be durably produced in aqueous dilutions of the Human Imunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated by antiretroviral therapy and having no detectable viral RNA copies in their blood. We suggest that the treatment of AIDS patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are discussed. Key words: DNA, Electromagnetic signals, bacteria

Centre Intgr de Recherche Bioclinique dAbidjan

World Foundation of AIDS Research and Prevention

R. Olivier, Cl. Lavallee, H.Chenal, M. Mbamy,

Nanectis Biotechnologies SA
J.Aissa, Cl.Lavallee, R.Olivier

Goettingen University and Chronix Biomedicals

E. Schutz, H.Urnovitz

University of Washington, Seattle

Gerald H. Pollack

Absence of evidence is not evidence of absence

Carl Sagan