Professional Documents
Culture Documents
Prevent Spoilage
Prevent Contamination
Bactericidal
Ability to kill microorganisms
Terminology, cont.
Sterilization
Destruction of all forms of microbial life including endospores
Methods of Control
Physical
Heat Moist heat is most effective (pasteurization and canning) Cold Slows bacterial growth Can be used for long term storage
Terminology
Disinfectant
Destruction of vegetative pathogens on inanimate objects
Antiseptic
Chemical agent designed for topical application to the body surface
Sanitizing Agent
Disinfectant that accomplishes some degree of cleaning Lowers microbial count on eating/drinking utensils to a safe public health level
Todays Procedure
Agar disc diffusion
Thoroughly swab a TSA plate with assigned bacterium made to a 0.5 McFarland Divide plate into quadrants with marker Add 5 impregnated paper discs to the plate as described by the instructor Incubate at 37C for 24 hours
Chemical agent
Control
Grab 10 tubes containing 1.0 ml of TSB (label each tube) Make a serial dilution of your chemical (control tube does not receive any of your chemical) Add 0.1 ml of your assigned organism made to a 0.5 McFarland to each tube Incubate tubes at 37C for 24 hours