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Research Proposal
IDENTIFICATION AND
ISOLATION OF PROBIOTIC
MICROORGANISM IN EEL
Methyl
Grams
Red Catalas
stainin
test e test PCR
g
(MR)
Lactic acid producing bacteria (probiotic
bacteria) isolated from eel digestive tract
Then the animals were
The eel fish were dissected to remove
The eel fish was collected washed with sterile
from fresh water river. the digestive tracts by
distilled to remove the the sterilization
unwanted particles. condition.
pour plated on
After centrifugation the
nutrient agar plate The digestive tracts
supernatant was taken
and incubated for 24 were homogenized in
and serially diluted in
h at room peptone broth for
sterile distilled water in
temperature. centrifugation.
the test tubes to 10-2, 10-
3
, 10-4, 10-5, 10-6, 10-7 and
10-8
Individual colonies
Then the The single
were taken and broths were colony is
inoculated in incubated for subject to lab
McConkey broth.
18-24 h at identification
37°C.
GRAM STAINING
METHYL RED TEST
CATALASE TEST
PCR IDENTIFICATION
DATA COLLECTION
Data will be collected from February 2009 until November 2009. Qualitative
data will be collected via journal and internet research or any other media.
The qualitative data is more on focusing into literature review and previous
research on the same study.
For the quantitative data, they will be collected from the lab experiment
which will be conducted during the research. It will include morphological
analysis, biochemical analysis and Polymerase Chain reaction (PCR)
analysis. The data will be collected from at least 20 samples to get the exact
result.
COSTING AND EXPENSES
Item Cost
Live eel RM20.00/kg
Medium (Mc Conkey) RM 250.00
Item Cost
Other necessary Live eelchemical and RM 150.00
RM20.00/kg
apparatus which may not
Medium (Mc Conkey)
Other necessary chemical and apparatus
RM 250.00
RM 150.00
available
which in lab
may not available in lab
Taq buffer RM250.00
TaqMgCl2
buffer RM250.00
RM250.00
dNTP’s
MgCl2 RM250.00
RM250.00
Forward primer RM250.00
Reverse primer
dNTP’s RM250.00
RM250.00
Taq polymerase RM250.00
Forward primer
Grand Total RM250.00
RM1920.00
LITERATURE
REVIEW
DATA
COLLECTION
LAB WORK
DATA
ANALYSIS
THESIS
WRITING
THESIS
SUBMITTION
CONCLUSION