In-feed antibiotic effects on the swine intestinal microbiome

Adventist University of the Philippines

Demetillo, V., Kairupan, M.,, Salindeho, A., Serdenia, A., Usman, F.

Antibiotic
coined by Selman Waksman in 1942. antagonistic to the growth of other microorganism.

 disease treatment disease prevention growth promotion

Copyright 2008 PresentationFx.com | Redistribution Prohibited | Image 2008 clix/sxc.hu | This text section may be deleted for presentation.

Copyright 2008 PresentationFx.com | Redistribution Prohibited | Image 2008 clix/sxc.hu | This text section may be deleted for presentation.

Bacteria that inhabit the GI tract of animals :

Intestinal microbiota
nutrient extraction
immune system and epithelium development defense against pathogens

Copyright 2008 PresentationFx.com | Redistribution Prohibited | Image 2008 clix/sxc.hu | This text section may be deleted for presentation.

Gut microbiota
may antagonize future disease treatment reservoir of resistant genes for animals and food-borne pathogens

Collateral Effects of Antibiotics:

increase in E. coli populations abundance of antibiotic resistant genes

Medicated pigs

Non-medicated pigs

receives a diet containing ASP250 antibiotic

same diet but without antibiotics

Copyright 2008 PresentationFx.com | Redistribution Prohibited | Image 2008 clix/sxc.hu | This text section may be deleted for presentation.

ASP250
Chlortetracycline Sulfamethazine Penicillin

Copyright 2008 PresentationFx.com | Redistribution Prohibited | Image 2008 clix/sxc.hu | This text section may be deleted for presentation.

Objective
To prove the increase of antibiotic resistant genes of bacteria in medicated swine.

MATERIALS
Pigs

Techstart 17-25; Kent feeds Antibiotic (ASP250) *chlortetracycline- 100g/ton *sulfamenthazine-100g/ton *penicillin-50g/ton

Fecal sample

METHODS
Choose the swine

DNA Sequencing

Phylotype Analysis

Metagenomic Analysis

Quantitative PCR

Statistical Analysis of qPCR result: Abundance and Diversity

RESULTS
Shifts in community membership with ASP250

Shifts in functional gene abundance with ASP250

 Pervasive antibiotic resistance in the Absence of antibiotic exposure.

Nonmedicated
50 different resistant genes.
5 were detected in 66% of samples

Medicated
38 genes were found in one Medicated Sample
19 were detected in 66% of samples 10 were detected in at least 8 or 9 of the samples.

qPCR and Metagenomic analyses reveal shifts in Resistance gene richness and abundance in Medicated pigs.

CONCLUSION
In feed antibiotic in swine increased the diversity and abundance of antibiotic resistance genes. In feed antibiotic increases the abundance of E. coli.

no exposure of sow or piglets in antibiotics except for the treatment.

antibiotics are used to treat and prevent disease in swine, to protect swine welfare, and to improve growth rate and efficiency of feed utilization.

Thank You!!!