1

About us Our experience in nanotechnology

Synthesis methods Lab for synthesis & characterization Projects Publications Participants

Antibacterial surfaces Conclusions

A materials research group that belongs to the Faculty of Science, Universidad Autnoma de San Luis Potos. Our current research interests are in the field of nanotechnology. These research interests have emerged according to the projects we have been developing in this field. All these interests are related to the synthesis, characterization and applications of nanoparticles.

- Dynamic of nanoparticles (Growth and forming studies) - Large scale productions methods -NPs antibacterial properties - Cytotoxicology of NP`s - Colloidal dispersions

Formally we started our work in the area of nanotechnology in 2004 exploring different methods for the synthesis of metal nanoparticles. In particular, this project was started at the request of one of the largest mining companies in Mexico. In the first stage we explored different methods of synthesis at the laboratory for four different materials, silver, gold, zinc oxide, and magnesium hydroxide.

10

11

12

13

14

15

16

Zn ZnO or Zn(OH)2

17

18

19

20

Titanium oxide(TiO2) Silver Gold Copper Platinum Zinc oxide

21

Alongside with the methods of synthesis for nanoparticles we develop. We have implemented a methodology for nanoparticle characterization. This sounds like something obvious but it is necessary to have a documented methodology for a long term projects or when you develop methods for others.

22

23

2.4 2.0

Silver

max433 nm

1.6 1.2 0.8 0.4 0.0 -0.4 300

H L N P G I K M O 12 horas

Absorbance

350

400

450

500

550

600

650

Longitud de onda (nm)

24

25

26

27

13

10 8 Exo

12

TGA

6 4
CDB

Flujo de calor (mW)

Peso (%)

2 0 -2 -4

11

10

-6

-8 Endo
9
0 50 100 150 200 250 300

-10

Temperatura (C)

28

SEM

30

31

32

During the course of nanotechnology-related projects, we have built the necessary infrastructure for the continuation of such projects. We have also been expanding our infrastructure to solve problems related to applications that we have been explored.

33

34

We have designed and implemented pilot plants to produce NPs (silver and TiO2:Ag

35

36

37

FACTOR Microorganism Quantification

CONDITION Overnight culture Spectrometric, bacteria 620 nm and fungus 530 nm, 0.5 McFarland Bacteria grown in Mueller Hinton Broth and fungus grown in RPMI Determination of Minimum Bactericidal Concentration (MBC) All experiments in triplicates

Culture media

Endpoints

Clinical and Laboratory Standards Institute (CLSI), M2-A9 and M7-A7, 2006 NCCLS, Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes, M24-A,2003 National Committee for Clinical Laboratory Standar (NCCLS), M27-A, 1997

MICRODILUTION PLATE
1 2 3 4 5 6 7

a) 50 L MH
8
9 10 11 12

BLACK
GREY WHITE BROWN
b) 50 L Nanoparticle c)Dilutions d)50 L microorganism

MBC: Minimum Bactericidal Concentration test MFC: Minimum Fungicidal Concentration test
MBC and MFC are the lowest concentrtion of nanoparticles that kills 99.9% of the bacteria or fungi.

PC
25 mg/ mL
0.42 mg/ mL 0.105 mg/ mL 6.25 mg/ mL 12.5 mg/ mL

MBC

MFC

0.21 mg/ mL

Clinical and Laboratory Standards Institute (CLSI), M2-A9 and M7-A7, 2006 NCCLS, Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes, M24-A,2003 National Committee for Clinical Laboratory Standar (NCCLS), M27-A, 1997

Minimum Bactericidal Concentration (MBC)

S.aureus MRSA E.coli P.aeruginosa A.baumanii M.smegmatis B.subtilis C.albicans
10m L

MBC

MIC

This is the disc diffusion method (ZOI, Kirby Bauer) Used to demonstrate activity / potency of antimicrobials or antibiotics by measuring the zone of inhibition against specified microorganisms. The test is applicable for materials treated with an antimicrobial agent that leaches out of the material.

42

43

In these last years we have participated with different companies trying to implement applications based on nanotechnology

45

47

Previous projects

49

50

51

Working with industries in applied research is not easy They have different rhythm of work They do not want to deviate from the original objectives They are not interested in publishing results

52

54

55

Ceramics

WC units, tiles and joint fillers for tiles

Plastics Paints

56

Synthesis methods for low cost materials Scaling up Aging/stabilization of nanoparticles

oxidation and/or fragmen

tation

Environmental implications of nanoparticles

57

Bio assays
Antimicrobial
Cytotoxicity Antimicrobial

mechanism of nanoparticles (metallic)

Biofilm inhibition

58

59

60

The ceramic industry in Mexico is a very important sector and is currently seeking for innovative products. Especially they are looking for ceramic surfaces with different functionalities

61

Sanitarios Lamosa is a company dedicated to the design, manufacturing and commercialization of ceramic bathroom fittings, oriented on the residential and institutional use markets.

62

63

Explorative project with a large Mexican paint maker company:

Evaluating commercial additives Adapting and validating microbiological assays for paints Testing inorganic additives (nanoparticles) Designing organic/inorganic additives

64

Explorative project with a large Mexican paint maker company:

Evaluating commercial additives Adapting and validating microbiological assays for paints Testing inorganic additives (nanoparticles) Designing organic/inorganic additives

65

Caracterizacin

Sntesis de las nanopartculas Mtodo de reduccin

DRX, MET, DDL, UV-Vis

Pruebas Bactericidas Mtodo de microdilucin en placa

Elaboracin de las pinturas Con incorporaci n de Nps

Pruebas Bactericidas Protocolo de las placas petrifilm 3M

Pruebas colorimtricas Desviacin del color (Espacio de color CIE L*a*b*)

Pintura

Nanopartcula s

Pintura con efecto bacteriosttico

a)

c)

e)

b)

d)

f)

Resultados de placa petrifilm de un muestreo sobre superficies: a), b), c), d) es el recuento de bacterias; a) mesa y b) pared de cafetera, c) piso y d) lavabo de un bao, e) y f) corresponde al recuento de hongos del piso y el lavabo respectivamente.

Resultados de concentracin inhibitoria mnima de nanopartculas de compsitos TiO2@Ag.

Concentracin inhibitoria mnima (mg mL-1) Bacteria S. aureus (R) Muestra 1_1 2_2 Nanopartculas de Ag M2_ 1, 10:1 MS_1@Ag 2, 10:1 M!, 25:1 M2 2,2 5:1 M1 14 50:1 M 2 50:1 M 1 10:1 M 2 10:1 M 14 25:1 M 2 25:1 M 14 50:1 M 2 50:1 E. coli Concentracin del Concentracin de Concentracin del Concentracin de compsito Ag en el compsito compsito Ag en el compsito ----* No ----* No ----* No ----* No No 16.67 No 13.02 333.33 129.10 33.10 354.17 166.15 35.16 375.00 136.93 28.12 395.83 173.59 29.68 250.00 0.0000 1.250 135.42 61.45 0.68 270.00 122.90 1.080 208.33 64.55 0.83 400.00 136.93 1.40 333.33 129.10 1.13 416.67 129.10 0.86 375.00 136.93 0.86 208.33 64.55 20.68 187.50 68.47 18.61 229.17 51.03 19.25 187.50 68.47 15.75 291.67 102.06 1.46 166.67 64.55 0.83 291.67 102.06 1.46 270.83 122.90 1.35 416.67 129.10 1.42 458.33 102.06 1.56 416.67 129.10 1.29 458.33 102.06 1.42

datos para cada bacteria: CIM promedio del compsito (mg/mL) y la desviacin estndar (primera columna) y concentracin de Ag en el compsito (mg/mL) (segunda columna). * En estas muestras no se encontr actividad bactericida a las concentraciones experimentadas.

Pruebas bactericidas sobre superficies de pintura

Resultados de pruebas bactericidas sobre superficies pintadas, utilizando placas Petrifilm. Muestra Pintura formulada (sin NP2) Pintura formulada (Sin Np2) Pintura + Nanopartculas de Ag Pintura + M 1 10:1 Pintura + M 125:1 Efectividad (%) 64.48 7.61 67.56 5.67 66.02 2.66 93.44 1.72 99.11 0.84

n=6

Contact us

(2) Servicios Administrativos Peoles, S.A., Mxico, the Coordinator in Mxico

(3) Danmarks Tekniske Universitet, Denmark (4) Centro de Investigacin en Quimica Aplicada, Mxico

(5) Centro de Investigacin en Materiales Avanzados, S.C., Mxico

Extranet for participants (password needed)

(6) SO.F.TER S.p.A., Italy

(7) Universidad Autnoma de San Luis Potosi, Mxico

(8) SO.VE.RE. S.p.A., Italy

(9) Owens Corning Mexico S.de R.L.de C.V., Mxico (10) Owens Corning Fiberglas A.S. Ltda, Brazil

21/05/12 07:53

71

Events

(password needed)

uality mined nanomaterials mass produ...

Contact us http://minano.vtt./participants.htm

(6) SO.F.TER S.p.A., Italy

(2) Servicios Administrativos Peoles, S.A., Mxico, the Coordinator in

(7) Universidad Autnoma de San Luis Potosi, Mxico

(3) Danmarks Tekniske Universitet, Denmark

Participants

(4) Centro de Investigacin en Quimica Aplicada, Mxico (8) SO.VE.RE. S.p.A., Italy

(1) VTT Technical Research Centre of Finland, the Coordinator

Extranet for participants (password needed)

(9) Owens Corning en Materiales Avanzados, S.C., Mxico (5) Centro de Investigacin Mexico S.de R.L.de C.V., Mxico (10) Owens Corning Fiberglas A.S. Ltda, Brazil

(6) SO.F.TER S.p.A., Italy

(2) Servicios Administrativos Peoles, S.A., Mxico, the Coordinator in Mxico

1 of 2

(3) Danmarks Tekniske Universitet, Denmark (4) Centro de Investigacin en Quimica Aplicada, Mxico

(7) Universidad Autnoma de San Luis Potosi, Mxico

(8) SO.VE.RE. S.p.A., Italy

(5) Centro de Investigacin en Materiales Avanzados, S.C., Mxico

participants needed)

(9) Owens Corning Mexico S.de R.L.de C.V., Mxico (10) Owens Corning Fiberglas A.S. Ltda, Brazil
(6) SO.F.TER S.p.A., Italy

1 of 2

72

47.62 g/g (microgramos de nanopartculas de plata por gramo de poliestireno =0.0048% en peso

Proceso de incorporacin de nanopartculas en la matriz polimrica;

73

Proceso de extrusin y peletizado del nanocompsito; a) perfil de temperatura de extrusin

Probetas de evaluacin para pruebas mecnicas y antimicrobianas

74

UFC OM07 (Testigo) 4.27E+06

%Inhibicin

Confiabilidad

OM08

6.67E+04

98.4%

1.81

0.02

BF09

9.33E+04

97.3%

1.58

0.01

BF10

3.33E+04

99.2%

2.11

0.01

BF11

0.00E+00

100%

---

---

R: Reduccin logartmica, UFC: Unidades Formadoras de Colonias

NOTA:
75

We are still at the learning stage Conventional Industries (cement, plastics, paints, textiles manufacturers) are demanding application based in nanotechnology In Mexico the nanotechnology is a field of opportunities

80