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Intestinal Microsporidiosis (Desportes, 1985) Emerging opportunistic infection (HIV infected +++) Microsporidia pathogens: Protozoa / Fungi
1st descriptions in Tunisia in 1997: HIV patients, Congenital immunodefisciency & Immunocompetent child
Studied Subjects
Techniques
Direct Exam
(Ritchie concentration)
Trichrome/Weber Staining
PCR V1/PMP2
279 pb
250 pb
250 pb E. bieneusi, 270 pb Enc. intestinalis, 268 pb Enc. cuniculi, 279 pb Enc. hellem
Sequencing
375 pb 353 pb
Results
Prevalence in HIV patients Trichrome VIH (+) (123) 8 (6.5%) PCR 18 (14.6%) p 0,02
5 (9.3%)
Species
E. bieneusi (n= 8) Enc. intestinalis (n = 10) Enc. hellem (n = 1)
Proportion
42.1% 52.6% 5.3%
Patient
PCRs
PCR (V1/PMP2)
R42 R44 R106 R56 R14 R110 R119 Sce1 I14 R60 R92 I44 R68 R82 R86 R104 R117 I11 R15 250 pb 250 pb 250 pb 250 pb 250 pb 250 pb 250 pb 250 pb 270 pb 270 pb 270 pb 270 pb 270 pb 270 pb 270 pb 270 pb 270 pb 279 pb 270 pb 250 pb 270 pb 270 pb 270 pb
Sequencing
E. bieneusi E. bieneusi E. bieneusi E. bieneusi E. bieneusi E. bieneusi E. bieneusi E. bieneusi Enc. intestinalis Enc. intestinalis Enc. intestinalis Enc. intestinalis Enc. intestinalis Enc. intestinalis Enc. intestinalis Enc. intestinalis Enc. intestinalis Enc. hellem Enc. intestinalis ND ND ND ND
V1/EB450 E. bieneusi
+ + + + + +
-
+ + + +
-
+
-
A8
240 pb ND
ND
+ 7 (18.4%) 11 (12.9%) 18
31 74 105
38 85 123
The infection was significantly associated with low T Lymphocyte CD4 cell counts (p=0,008)
Patient
I11 I14 I22
Age
51 55 ND
Stools
Diarrheal Diarrheal Diarrheal
Disease
Myeloma Myeloma Myeloma
I44
A8
35
ND
Diarrheal
Molded
Myeloma
BM aplasia
CONCLUSION
High
infection
in
PCR much more sensitive than Microscopy First microsporidia species identification in Tunisia: E. bieneusi & Enc. intestinalis; but also Enc. hellem Co-transmission of Genotype B (Anthroponotic) et D (Zoonotic) of E. bieneusi
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