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www.rsc.org/metallomics | Metallomics

Incorporation of excess gadolinium into human bone from medical contrast agents
Thomas H. Darrah,*ad Jennifer J. Prutsman-Pfeier,b Robert J. Poreda,a M. Ellen Campbell,ad Peter V. Hauschkac and Robyn E. Hannigand
Received 13th March 2009, Accepted 25th August 2009 First published as an Advance Article on the web 16th September 2009 DOI: 10.1039/b905145g We nd anomalously high gadolinium (Gd) concentrations in the femoral head bones of patients exposed to chelated Gd, commonly used as a contrast agent for medical imaging. Gd is introduced in chelated form to protect patients from exposure to toxic free Gd3+, a calcium antagonist which disrupts cellular processes. Recent studies suggest Gd chelates break down in vivo, and Gd accumulation in tissue is linked to medical conditions such as nephrogenic systemic brosis (NSF), acute kidney failure, and in some cases death. We measure Gd and other rare earth element (REE) concentrations in 35 femoral heads by solution based ICP-MS. Gd concentrations in patients with documented exposure to Gd-based contrast agents (n = 13: Gd DTPA-BMA (Omniscan) n = 6; Gd HP-DO3A (Prohance) n = 5; unknown type n = 4) are signicantly higher (p o 0.001) than the control group (n = 17). We use our control group to establish the natural background level of Gd in human bone (cortical 95% CI: 0.023, 0.041 nmol/g; trabecular 95% CI: 0.054, 0.107 nmol/g). A control group outlier reveals the occurrence of individuals with high concentrations of all REEs, including Gd. Because of this, we calculate Gd anomalies from the concentrations of adjacent REEs and normalize to the control group mean to isolate Gd input from contrast agents. Normalized Gd anomalies, (Gd/Gd*)N, for exposed patients range up to 4800 times the natural level (95% CI: 124, 460). Our data conrm that Gd, introduced in chelated form, incorporates into bone and is retained for more than 8 years. No dierence was observed in bone Gd concentrations and anomalies between patients dosed with Gd DTPA-BMA (Omniscan; n = 6) and Gd HP-DO3A (Prohance; n = 5). Osteoporotic fracture patients exposed to Gd have signicantly lower Gd concentrations than osteoarthritis patients (p o 0.001). This indicates dierent mechanisms of metal incorporation and/or retention in osteoporotic bone tissues, and may signal an increased risk of endogenous Gd release for patients with increased rates of bone resorption (e.g. osteoporosis patients and menopausal, pregnant, and lactating women) who are exposed to Gd-based contrast agents.

Introduction
The use of contrast agents has expanded the role of imaging in medical diagnostic studies,1 and hence the size of the population exposed to chelated Gd. In fact, since 2005 more than 20 million Gd-based contrast enhancement procedures are performed each year.2 Gd (z = 64) is a paramagnetic rare earth element (REE) commonly used as a contrast enhancement agent for clinical and diagnostic medical imaging.35 Gd was the rst contrast agent introduced for diagnostic MRI studies because of its paramagnetic nature with an ecient (long) T1 relaxing mechanism.6 Free gadolinium (Gd3+) is extremely
a

Department of Earth and Environmental Sciences, University of Rochester, Rochester, NY 14627, USA. E-mail: Thomas.Darrah@umb.edu b Department of Autopsy and Neuropathology, University of Rochester Medical Center, Rochester, NY 14624, USA c Department of Orthopedic Surgery, Childrens Hospital Boston, Harvard Medical School, Boston, MA 02115, USA d Department of Environmental, Earth and Ocean Sciences, University of Massachusetts at Boston, Dorchester, MA 02125, USA

toxic,7,8 disrupts cellular processes,9,10 inhibits stretch-activated ion channels,11 and is one of the most ecient known calcium antagonists.9,12,13 Chelation of Gd in the MRI contrast media is intended to prevent toxic cationic Gd (Gd3+) from directly interacting with cells and tissues, and to allow ecient and rapid removal of the contrast agent from the body following imaging studies.6,14,15 Severe adverse health eects may occur with the use of Gd-based contrast agents if Gd dissociates from chelate structures in the blood stream. Gd3+ release from the chelating agent is known to occur via transmetallation reactions in which metals in blood and extracellular uids (e.g. Cu, Zn, Fe, Ca) replace Gd within the chelate structure.2,1618 Anions such as PO42; CO32; OH compete with the chelates for the Gd cation.2,11 Transmetallation reactions also release Gd3+ from Gd DTPA-BMA during transport across the cellular membrane, concentrating ionic Gd3+ within cells.19 Previous studies have shown that excretion of the majority of Gd-chelates occurs within 90 minutes of injection, from which the authors concluded that Gd is rapidly removed from
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the body.2022 Other studies document toxicity resulting from exposure to Gd-based contrast agents, linking them to symptoms such as nephrogenic systemic brosis (NSF),1,17,23,24 anaphylactic shock,25 acute kidney failure,21,26 and in some cases death in patients who had previously compromised kidney and immune function.27 These studies indicate that some of the Gd chelate agent may degrade quickly after injection, allowing release and incorporation of free Gd3+ into the body, e.g. into the skin tissue of nephrogenic systemic brosis patients.2830 Gadolinium concentrations averaging 2.8 and 7.5 nmol/g were incorporated into bone tissues of patients exposed to Gd DTPA-BMA (Omniscan) and Gd HP-DO3A (Prohance) three days prior to hip replacement surgery.31 Human bone mineral (a carbonated hydroxyapatite) serves as a reservoir for the majority of the bodys Ca (99%), P (80%), Mg (50%), and other trace metals.3234 Metals incorporate into bone by two major routes: (1) active incorporation during osteoblast-mediated bone mineralization, and (2) passive ionic exchange into the crystal lattice of individual bone mineral crystals (23 nm 2040 nm platelets).3537 The [calcium] [phosphate] ion product in extracellular uid is supersaturated with respect to bone mineral.33 During active incorporation, osteoblastic membrane transport from the extracellular uid regulates the delivery of these ions to the mineralizing collagen-rich organic matrix of developing bone, along with lesser amounts of other metal cations present in the uid (Mg, Sr, Pb, Gd, other REE, etc.). Ionic exchange with the bone mineral lattice is dependent on the relative concentrations of trace metal cations, ionic t in lattice vacancies, and individual solubility products of their phosphate and carbonate salts.11 Metals are also released from bone during bone resorption. Multinucleated osteoclasts secrete HCl into membrane-sealed resorption pits on the bone surface, lowering the pH to about 4 and completely dissolving the bone mineral under these specialized cells. This maintains calcium homeostasis in the blood, as well as removing bone targeted for replacement during normal remodeling.33,34,38,39 Bone turnover in normal adults is in the range of 515% of total bone mass per year, but rates for resorption of the trabecular (spongy) bone in postmenopausal women are 1926%.40 Therefore, Gd incorporated into bone, whether as free Gd3+ or as a chelate, may potentially be endogenously released back into the bloodstream by bone resorption and remodeling processes, as has been shown for other trace metals.34 For example, stable isotopic compositions of blood Pb have shown that up to 70% of plasma Pb is released endogenously from bone tissue,41 with increased plasma Pb concentrations in pregnant and lactating women, who experience increased bone resorption.42,43 We investigate the relation between Gd concentrations in trabecular and cortical bone tissues and medical exposure to Gd chelate contrast agents. Femoral heads removed during hip replacement surgeries are analyzed for Gd and other REEs to identify anomalous Gd concentrations. The eects of bone pathology on metal incorporation and retention are investigated by comparing Gd concentration in the bone of osteoporotic fracture patients to those of osteoarthritis patients.
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Experimental
Sample selection Bone samples consist of femoral heads obtained from patients who underwent total hip replacement surgery at the University of Rochester Medical Center, prior to specimen disposal (RSRB approval #0019010). Clinical histories associated with the samples are limited to the occurrence or lack of occurrence of Gd-based contrast agent exposure, some brand information, the reason for hip replacement surgery, and routine personal data, e.g. gender, age. The control group includes 18 osteoarthritis patients with no exposure to Gd-based contrast imaging studies. The Gd exposure group includes 13 patients with medical records documenting exposure to Gd based contrast imaging studies, with maximum time elapsed since exposure of 8 years prior to surgery. Contrast agent type is known for 11 of these patients: the linear, non-ionic, Gd-chelate2 Gd DTPA-BMA (Omniscan), n = 6; and the macrocyclic non-ionic, Gd-chelate2 Gd HP-DO3A (Prohance), n = 5. Bone samples from 4 additional patients have anomalous Gd concentrations but incomplete associated medical records with no Gd exposure histories. These 4 patients are not included in comparisons of control and exposure group. Based on our inference that they have been exposed to Gd contrast agents (see results), we include these 4 patients in our comparison of osteoarthritis and fracture patients exposed to Gd. Table 1 summarizes the groups. Sample preparation Sample preparation was conducted in a class 100 trace metal free clean laboratory facility. Femoral heads were crosssectioned to a thickness of approximately 10 mm using a low speed diamond tip Isomet saw. Samples were dried for 48 hours (or until dry) at 80 1C in a vacuum oven. Trabecular and cortical bone samples were extracted using a manually operated precision Dremel tool with graphite carbide tip to provide approximately 300 mg of each bone tissue type per patient. Care was taken to avoid anomalous sections of bone, such as areas near fracture zones, areas of necrosis, zones of hemorrhage, or arthritic nodules. Fifty milligram (50 mg) aliquots of cortical and trabecular tissues from each patient were dried and then crushed with a ceramic mortar and pestle. After crushing, sample weights were recorded to 0.001 mg, and dried bone material was digested in Teon vessels using concentrated (15.9 mol L1) ultra-pure nitric acid (HNO3) at 90 1C for approximately 5 hours or until digestion was complete. The remaining solution was baked for
Table 1 Patient groups according to medical histories of exposure to Gd based contrast agents. OA = osteoarthritis; F = fracture. The absence of fracture patients (F) in the control group reects the diculty of retrieving complete medical records for emergency surgery patients Control group n = 18 OA = 18 F=0 Gd exposure group n = 13 OA = 7 F=6 Incomplete medical histories n=4 OA = 2 F=2

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approximately 3 hours (or until dry) at 90 1C to remove excess nitric acid. Residual dried material was then redigested with ultra pure HNO3. Internal standards consisting of known quantities of indium (In) and bismuth (Bi), used to correct for instrumental drift, were added to the digested bone material. The solutions were then diluted using water puried to 18.2 MO cm resistance (by a Milli-Q water purication system, Millipore, Bedford, Mass., USA) to result in a nal concentration of 2% nitric acid (by volume), and 1 ng/g of each of the internal standards (In and Bi). All standards used during ICP-MS analysis, including aliquots of the certied NIST 1486 SRM and procedural blanks, were prepared in an analogous fashion. Quantication of NIST 1486 SRM bone powder Accurate determination of trace element concentrations by ICP-MS requires the use of a directly matrix-matched standard,44 with a similar major chemical composition and mineralogical form to the sample. Although the National Institute for Standards and Technology (NIST, USA) does currently certify two modern bone standard reference materials (SRMs), NIST SRM 1400 and NIST SRM 1486, for specic major and minor elements, no current standard allows for the quantication of many biologically relevant trace and rare earth elements, including those of interest in this study (Sm, Gd, Tb).

We use standard addition to quantify Sm, Gd, and Tb concentrations in NIST 1486 SRM, which consists of powdered bone meal that approximates the matrix of the sample unknowns. Standard addition quantication eliminates potential eects of variable dilution of the matrix and any associated matrix matching eects. The standard addition procedures used are as follows: (1) Aliquots of NIST 1486 SRM were prepared using preparation techniques identical to those used for bone samples. (2) Known quantities of pure element standard solution were added to six aliquots, while one was left with unaltered NIST 1486 SRM to produce an array of samples with nal concentrations of 0, 0.1, 0.5, 1, 5, and 10 ng/g for each analyte, with duplicate aliquots at 0.1 ng/g concentration. (3) Each sample, with a known concentration of NIST 1486 SRM material and known quantities of added element standard, was then analyzed by ICP-MS, giving the intensity of each analyte in counts per second. Five replicate analyses of each sample were performed and values averaged to result in a mean intensity for each analyte. (4) For each element, mean intensity (counts per second) of each sample was plotted against the amount of known standard added. The data were then regressed to determine the initial concentration of each analyte in NIST 1486 SRM. The regression for Gd is shown as an example in Fig. 1. Table 2 shows the calculated concentrations of Pb, Sm, Gd, and Tb in bone powder (NIST 1486 SRM), as well as the previously certied concentration of Pb. Results obtained by this study for Pb are in agreement with the certied value (o1s) as reported by NIST, USA.45

Analytical procedures and uncertainty REE concentrations were measured using a Thermo X7 ICP-MS. Solution based ICP-MS analyses were conducted with modications to the EPA 6020A methodologies,46,47 the approved ICP-MS analytical procedure for inorganic trace elements in soils and sediments. To clean sample lines and reduce memory eects, sample lines were washed with 2% nitric acid solution for 120 seconds between analyses. Procedural blanks were analyzed within each block of 8 samples, to monitor and correct for instrumental and procedural backgrounds.

Fig. 1 Standard addition linear regression for Gd concentration in NIST 1486 SRM.

Table 2 Concentrations of Sm, Gd, and Tb in bone powder (NIST 1486 SRM) as determined by standard addition, and comparison of calculated Pb with previously certied Pb value.45 Note that the Pb concentration determined in this study by standard addition is o1s from the certied value (a) Calculated element concentrations (this study) Element Pb (ng/g) Sm (ng/g) 10.57 0.11 Gd (ng/g) 10.44 0.11 Tb (ng/g) 6.12 0.07

Conc. 1329 St. dev. 12.2 (b) Previously certied Pb concentration (NIST, 2007) Element Conc. St. dev. Pb (ng/g) 1335 14

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Calibration standards used to determine REE concentrations in bone samples included aliquots of NIST 1486 SRM spiked with known quantities of REEs in a linear range from 0.1 to 10 ng/g. Standards were prepared from 1000 mg/L single element standards obtained from SCP Science, USA. Concentrations of REEs in NIST 1486 were quantied by standard addition as described above. Unaltered aliquots of NIST 1486 SRM were analyzed as unknowns to determine external precision as less than 5%. Isobaric corrections were performed on-line using ICP-MS software. Three duplicate analyses (n = 3) were performed for all analytes for each sample solution. Limits of detection

(LOD) according to Long and Winefordner48 are as follows: Gd = 0.000158 nmol/g; Sm = 0.0000124 nmol/g; Tb = 0.000554 nmol/g. Limits of quantication according to Long and Winefordner48 are as follows: Gd = 0.000189 nmol/g; Sm = 0.000022 nmol/g; Tb = 0.000556 nmol/g. Method detection limits were calculated according to the two-step approach using the t99SLLMV method49 at 99% CI (t = 3.71) as Gd = 0.00236 nmol/g, Sm = 0.00458 nmol/g, Tb = 0.00100 nmol/g. MDL values incorporate matrix eects and interferences associated with the complex bone matrix, and provide a more robust and accurate limit of detection for these analyses.

Table 3 Gd concentrations and anomalies for (a) control group (n = 18); (b) Gd exposure group (n = 13); and (c) statistical comparison of control and exposure groups. Also shown (d) are concentrations and anomalies for 4 additional patients with inferred Gd exposure. Gd anomalies are calculated according to eqn (1), using Gd concentrations measured in cortical tissues, and normalized to the control group mean (Gd/Gda)N (eqn (2)). OA = osteoarthritis; F = osteoporotic fracture. Mean, median, and 95% condence interval (CI) of the mean are listed for each data subset Cortical Patient ID # (a) Control group 2 5 6 9 10 11a 12 13 15 17 18 20 21 22 23 26 29 49 Control group statistics (b) Gd exposure group 16 25 33 35 36 68 85 1 75 79 81 87 91 Exposure group statistics Pathology Sm (nmol/g) Gd (nmol/g) OA OA OA OA OA OA OA OA OA OA OA OA OA OA OA OA OA OA Meana Mediana 95 % CIa 0.03 0.03 0.02 0.05 0.02 2.58 0.02 0.01 0.01 0.03 0.02 0.02 0.02 0.01 0.01 0.03 0.02 0.02 0.02 0.02 0.016, 0.027 0.03 0.02 0.05 0.05 0.02 1.86 0.02 0.01 0.02 0.06 0.02 0.02 0.03 0.04 0.01 0.04 0.07 0.02 0.03 0.02 0.023, 0.041 Tb (nmol/g) Gd/Gd* (eqn (1)) 0.02 0.02 0.03 0.02 0.02 0.25 0.02 0.02 0.02 0.02 0.03 0.02 0.02 0.02 0.01 0.03 0.02 0.03 0.02 0.02 0.020, 0.024 1.3 0.9 2.0 1.6 1.0 1.8 0.9 0.7 1.3 2.3 0.8 0.9 1.3 2.8 1.4 1.5 3.3 0.8 1.45 1.28 1.07, 1.83
a

Trabecular (Gd/Gd*)Na (eqn (2)) Gd (nmol/g) 0.07 0.05 0.15 0.10 0.05 6.54 0.06 0.04 0.04 0.19 0.04 0.05 0.18 0.06 0.02 0.12 0.10 0.04 0.08 0.06 0.054, 0.107 39.5 15.3 24.3 28.8 15.8 20.2 34.8 0.11 2.58 4.91 3.33 17.1 1.80 16.05 15.78 8.09, 24.0 o0.001 22.58 28.04 1.30 1.94

0.9 0.6 1.4 1.1 0.7 1.3 0.6 0.5 0.9 1.6 0.5 0.6 0.9 1.9 0.9 1.0 2.3 0.6 1.00 0.88 0.74, 1.26 826 295 181 832 512 274 318 1.1 56 99 93 277 29 280 197 124, 460 o0.001 414 405 21 27

OA 0.01 20.8 0.02 1198 OA 0.02 9.84 0.03 427 OA 0.01 6.24 0.03 262 OA 0.02 31.0 0.03 1207 OA 0.02 14.3 0.02 743 OA 0.02 10.1 0.03 397 OA 0.02 11.4 0.03 461 F 0.07 0.07 0.03 1.6 F 0.02 1.50 0.02 81 F 0.07 6.40 0.03 144 F 0.03 3.19 0.02 136 F 0.01 5.69 0.02 401 F 0.04 1.18 0.02 43 Mean 0.03 9.36 0.02 417 Median 0.02 6.40 0.03 293 95% CI 0.016, 0.040 4.09, 14.63 0.021, 0.028 180, 666 (c) 2 sample t-test comparison of control and exposure groups (normalized data (log(x)) p-value 0.77 o0.001 0.81 o0.001 (d) Inferred Gd exposure 27 OA 0.02 12.9 0.02 601 28 OA 0.02 14.4 0.03 587 80 F 0.04 0.90 0.03 30 84 F 0.04 1.06 0.02 40
a

Patient ID #11 is an outlier for all REE concentrations and is excluded from calculations of mean, median, and 95% CI of mean.

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Calculation of Gd anomaly We calculate the amount of excess Gd incorporated into bone from medical exposure as a Gd anomaly (eqn (1); Table 3), which is dened as the ratio of the measured Gd concentration in cortical bone tissue samples relative to the concentration expected based on the concentrations of adjacent REEs. Gd/Gd* compares measured Gd in bone to the expected value Gd* for normal dietary and environmental inputs based on other REEs, similar to the method used for rocks, soils and water.50 Because the rare earth element group (REEs) behaves in a coherent fashion, relative abundances remain nearly constant in natural systems (e.g. rocks, soils, water). The constants in eqn (1) normalize elemental abundance to upper continental crust (UCC) (i.e. most natural soils). Gd anomaly = Gd/Gd* = [Gd] M/[0.33[Sm] M + 0.67[Tb] M] (1)
Fig. 2 Rare earth element concentrations in cortical tissues for the control group (n = 18) and exposure group (n = 13) normalized to the upper continental crust (UCC) REE concentrations. The normal ranges for control and exposure groups are shown in grey and red, respectively (note that the dark red is overlap between control and exposure ranges). Outliers are shown separately. The occurrence of a control group outlier whose Gd concentration falls within the normal range for the exposure group reveals the necessity of calculating a Gd anomaly relative to abundance of neighboring REEs (eqn (1)). The outlier (patient ID #11) is anomalous for all REEs, and has a Gd anomaly similar to other control group patients.

Mean Gd anomaly for the control group is 1.45, similar to that observed for NIST 1486 SRM (Gd/Gd* = 1.38). This indicates that the concentration of Gd relative to Sm and Tb in normal human bone is almost 1.5 times higher than its relative concentration in upper continental crust. Exposure group Gd anomalies were divided by 1.45 to estimate excess Gd exposure beyond natural environmental inputs. Eqn (2) isolates the amount of Gd in bone tissue that results from medical dosing of contrast agents. A (Gd/Gd*)N value of 1 reects observed normal Gd incorporation. Normalized Gd anomaly = (Gd/Gd*)N = (Gd/Gd*)/1.45 (2)

Results
Gd concentrations (nmol/g) in trabecular and cortical bone tissues of the control group and exposure group are shown in Table 3, along with statistical summaries. The amount of excess Gd incorporated into cortical bone from medical exposure was estimated by calculating the Gd anomaly (Gd/Gd*) (eqn (1); Table 3), and normalizing to the control group mean (Gd/Gd*)N (eqn (2); Table 3).

Comparison of control and Gd exposure groups Fig. 2 shows the concentrations of all REEs for the control group (n = 18) and exposure group (n = 13), normalized to average upper continental crust (UCC), which approximates local Rochester, NY soil composition. The approximately at REE pattern seen for control group samples demonstrates the normal pattern of REE uptake relative to natural abundance in soils, food, and water. Gd concentrations in 17 of the 18 control group bone samples fall within a narrow range, with cortical tissue concentrations ranging from 0.013 to 0.070 nmol/g. One outlier (patient ID#11) has a cortical Gd concentration of 1.86 nmol/g (Table 3). For the control group (excluding the outlier, n = 17), the mean cortical tissue Gd concentration is 0.03 nmol/g (with a 95% CI of 0.023,
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0.041 nmol/g), and the median is 0.02 nmol/g (Table 3). Trabecular tissue concentrations range from 0.044 to 0.190 nmol/g, excluding the outlier (ID #11) of 6.54 nmol/g (Table 3). For the control group (n = 17), the mean trabecular tissue Gd concentration is 0.08 nmol/g (with a 95% CI of 0.054, 0.107 nmol/g), and the median is 0.06 nmol/g (Table 3). As shown in Fig. 2, patient ID #11 is an outlier for all REEs, not only Gd. The patients Gd anomaly is comparable to those of other control group patients (Gd/Gd* = 1.8). Average Gd anomaly (Gd/Gd*) for the control group is 1.45 (95% CI: 1.07, 1.83). Of the 13 patients in the exposure group, 12 have anomalously high bone concentrations of Gd with respect to the concentrations of other REEs (Fig. 2). Gd concentrations in the exposure group ranged up to 31 nmol/g in cortical bone with a mean of 9.36 nmol/g (95% CI 4.09, 14.63 nmol/g) and a median of 6.40 nmol/g. In trabecular tissues Gd concentrations ranged up to 39.51 nmol/g with a mean of 16.05 nmol/g (95% CI 8.09, 24.00 nmol/g) and median of 15.78 nmol/g (Table 3). One outlier (patient ID #1) has Gd concentrations and Gd anomaly comparable to the control group (e.g. 0.071 nmol/g cortical; Gd/Gd* = 1.3). Excluding the outlier, Gd anomalies (Gd/Gd*) in the exposure group range from 43 to 1207, with a mean of 417 (95% CI: 180, 666) (Table 3). Gd anomalies normalized to the control group mean, (Gd/Gd*)N, range from 30 to 832, with a mean of 280 (95% CI: 124, 460). Concentrations and anomalies have no correlation to time elapsed between Gd exposure and surgery, which ranges up to 8 years. Gd concentrations for both cortical and trabecular tissues, and Gd anomalies, are signicantly higher (p o 0.001) in Gd exposure patients (n = 13, including the outlier) than in
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Fig. 3 Statistical distributions of normalized Gd anomaly (Gd/Gd*N) for cortical bone samples of control and Gd exposure patient groups (p o 0.001), and for both osteoarthritis and osteoporotic fracture patients exposed to Gd, which are also signicantly dierent (p o 0.001; Table 4).

control group patients (n = 17) (Table 3). Concentrations of REEs other than Gd were indistinguishable from the control group (Sm: p = 0.602; Tb: p = 0.559). The relative distributions of Gd concentration in the control and exposure groups are shown graphically in Fig. 3. Comparison of Gd contrast agent brands Of the 11 exposed patients for whom Gd contrast agent brand information is available, 6 were given Gd DTPA-BMA (Omniscan), and 5 were given Gd HP-DO3A (Prohance). No signicant dierence in bone Gd concentration or Gd anomaly was observed according to brand of contrast agent (cortical p = 0.94; trabecular p = 0.99; cortical (Gd/Gd*)N p = 0.98). Dosage and frequency information were insucient to attempt correlation with bone Gd concentration. Patients with incomplete medical histories Four patients with incomplete medical histories have bone Gd concentrations and anomalies similar to those of the exposure group. While maximum (Gd/Gd*)N in the control group is 2.3, the 4 unknowns have (Gd/Gd*)N ranging from 21 to 414. These values occur at 450 sigma deviations from the control mean, and are very unlikely to result from anything other than exposure to Gd-based contrast agents.

Comparison of osteoarthritis and fracture patients exposed to Gd In comparing Gd incorporation into bone tissues of osteoarthritis and fracture patients, we exclude a statistical outlier with no Gd anomaly (patient ID #1), and include the 4 patients with inferred Gd exposure (Table 4). Fracture patients (n = 7) have signicantly lower Gd concentrations, in both cortical and trabecular tissues, and Gd anomalies than osteoarthritis patients (n = 9) (p o 0.001; Table 4), with no overlap in 95% condence intervals. Fig. 3 illustrates the dierent distributions of normalized Gd anomalies (Gd/Gd*)N for the two bone pathology groups.

Discussion
Gd incorporation into bone tissues Anomalously high Gd concentrations occur in both cortical and trabecular bone tissues of patients exposed to Gd-based chelated contrast enhancement agents (Gd-DPTA-BMA and Gd HP-DO3A). These results conrm the assertion by other authors that in vivo clinical exposure to Gd chelates results in Gd incorporation into body tissues.1,15,23 Other researchers have categorically demonstrated the potential toxicology, severe adverse health eects, and pathology associated with exposure to free ionic Gd3+.8,9,51,52 A previous study has

Table 4 Statistical summaries of concentrations and anomalies (cortical) for patients exposed to Gd according to bone pathology: (a) osteoarthritis patients (n = 9); and (b) osteoporotic fracture patients (n = 7). Concentrations and anomalies are signicantly lower in osteoporotic fracture patients (p o 0.001) Gd Cortical (nmol/g) Gd Trabecular (nmol/g) Gd/Gd* (eqn (1)) 654 587 392, 915 125 81 5, 245 o0.001 (Gd/Gd*)N (eqn (2)) 451 405 271, 631 86 56 3, 169 o0.001

(a) Osteoarthritis patients exposed to Gd (n = 9) Mean 14.33 25.49 Median 12.89 24.34 95% CI 8.54, 20.1 19.2, 31.8 (b) Fracture patients exposed to Gd (n = 7) Mean 2.84 4.71 Median 1.50 2.58 95% CI 0.695, 4.99 0, 9.89 (c) Statistical comparison: 2 sample t-test (normalized data (log(x)) p-value o0.001 o0.001

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shown that high clinical doses of chelated Gd, given immediately before hip replacement surgery, result in Gd concentrations measurable in the bone tissue averaging 2.8 (2.5) to 7.5 (4.9) nmol/g depending on the type of contrast agent administered.31 Our results for patients exposed to Gd-based contrast agents agree with this study, although our Gd values range up to three times higher, and include patients with up to 48 years time elapsed between exposure and surgical removal of the analyzed bone. We observe no correlation between elapsed time and bone Gd concentration. In addition, there is no signicant dierence in bone Gd concentration between patients exposed to Gd-DPTA-BMA (Omniscan; n = 6) and Gd HP-DO3A (Prohance; n = 5) in this study. In the environment, Gd exists in trace concentrations of approximately 3 to 6 mmol/g or less in most water, soil, and rock.53 However, existing data on naturally occurring Gd concentrations in human bone are sparse. Our results are consistent with one study on fossilized bone with very low Gd concentrations (0.011 to 1.00 nmol/g).54 Normal bone Gd concentrations, as represented by our control group, occur in a narrow range (95% CI: 0.023, 0.041 nmol/g in cortical tissue; n = 17). Our control group also includes an individual with concentrations of all REEs two orders of magnitude greater than those of other control group patients (Gd = cortical: 1.86 nmol/g; trabecular: 6.54 nmol/g). This occurrence indicates that it is necessary to measure all REE elements (at least Sm and Tb) to assess the magnitude of the Gd anomaly and determine medical exposure to Gd-based contrast agents. For example, without observing other REEs and calculating Gd anomaly, the control group outlier could be mistaken for an exposed patient. Our study documents the marked dierence between normal bone Gd concentrations and those of Gd-chelate exposed patients (up to 4800 times greater in the exposure group). Gd also remains elevated in both trabecular and cortical bone tissue for greater than 8 years, indicating retention of Gd in the body far longer than previously observed. Several previous studies assert that the in vivo use of Gd-based contrast agents is safe because of the rapid elimination of the chelated agent, with a half-life of only 30 to 60 minutes,21,22,31 without signicant release of free Gd3+.5,5557 Conversely, other recent publications indicate that chelated Gd breaks down in vivo, resulting in the release of Gd3+ to the blood stream and incorporation into the body.8,15,58,59 Gd3+ ion release from Gd-DPTA-BMA (Omniscan) has also been shown to occur via competitive transmetallation reactions on the cell membrane, mediated by ionic components (specically phosphate) in incubation media analogous to extracellular uid.19 To further address the question of Gd clearance, we have estimated the total skeletal Gd load in patients exposed to Gd contrast imaging procedures. Assuming 3670 g of dry, fat-free bone in a 70 kg male skeleton,60 and using the lower Gd value range for cortical rather than trabecular bone (Table 4) the total skeletal Gd ranges from 10 nmol (Fracture group) to 53 nmol (OA group). At a typical contrast agent dose of 21 mmol Gd-chelate for a 70 kg patient (0.3 mmol/kg), the Gd retained in the skeleton represents only 0.050.25% of the total imaging dose. Thus, while more than 99.7% of the total injected Gd appears to have been cleared, there is signicant long-term Gd retention by the skeleton.
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The process of bone dissolution by osteoclasts and new bone formation by osteoblasts steadily remodels the skeleton in adults. Annual rates of bone remodeling are typically 515% for the skeleton as a whole, but can exceed 25% for dynamically loaded trabecular bone in the hip.40 The Ca content of bone mineral is tightly regulated (26.4% of bone dry wt, 40% of ash wt) by the osteoblast and the stoichiometric requirements of the hydroxyapatite-type crystal lattice [Ca10(PO4)6(OH)2]. However, the incorporation of other elements (Mg, Sr, Pb, Gd, REE, etc.) is proportional to their concentrations in the extracellular uid or blood plasma,61 as well as their eciency of transport by the osteoblast, their ionic radius and steric t in vacancies in the crystal lattice, and the solubility products of their phosphate and carbonate complexes. Our data includes measurements of total Gd from complete nitric acid digestions of bone mineral without speciation methods. This analytical method does not allow conclusive identication of the Gd species incorporated into bone (chelate vs. other ionic complex vs. free Gd3+). Nonetheless our data clearly indicate that a small fraction of Gd does not clear the body following the application of Gd-based contrast agents and is incorporated and retained in bone for several years. We suspect that ionic Gd3+ is released from Gd-chelates by transmetallation reactions and subsequently incorporated into the carbonated calcium hydroxyapatite mineral phase of bone. In this proposed process Gd, a known Ca analog, may rapidly incorporate into bone mineral by substituting for Ca, given its similar ionic radius (Gd3+ 107.8 pm; Ca2+ 114 pm) and the high anity of rare earth elements to hydroxyapatite minerals.62 Gd incorporation by this process would result in the potential for toxic metal exposure to the cells within bone tissue (osteoblasts, osteoclasts, endothelial cells, hematopoietic bone marrow, etc.) e.g. Gd disrupts cellular processes and is a highly ecient calcium antagonist.9,12 The incorporation of Gd chelates or other Gd complexes into bone remains a possibility in the absence of suitable speciation methods. Whether Gd is incorporated into bone in chelated form or other Gd complexes, our data indicate that bone tissue eectively stores and sequesters Gd for at least 8 years. While this sequestration may protect vital organs, toxic metal release can occur during bone resorption and remodeling processes. During the resorption process osteoclasts secrete HCl acid to dissolve existing bone mineral, eectively lowering the conditions in the resorption compartment to an approximate pH of 4.33,39 Because the thermodynamic stability of Gd chelates is signicantly decreased at lower pH63 (i.e. there is increased potential for transmetallic exchange),63 normal osteoclast-mediated bone resorption presents an avenue for the release of ionic Gd3+, whether initially incorporated in the form of a Gd-chelate, other Gd complexes, or ionic Gd3+. Regardless, our data signals the need to consider both the potential eects of Gd incorporation on bone tissue itself, as well as the possibility of long term or time-delayed adverse health impacts associated with the use of Gd-based contrast agents. Gd incorporation into bone could negatively impact bone health similar to other toxic metals (e.g. Pb, Cd) which alter bone cellular processes, compromise bone health by
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altering bone cell signaling and molecular pathways,64 inhibit fracture healing,64 and alter the metabolism of osteoblasts and osteoclasts responsible for remodeling bone tissue.65,66 Impact of bone pathology on Gd incorporation Our dataset, which includes both osteoarthritis and osteoporotic fracture patients medically exposed to Gd-based contrast agents, provides a unique opportunity to investigate the eects of bone pathology on metal incorporation and retention in bone. Bone samples from fracture patients exposed to Gd have statistically lower Gd concentrations and Gd anomalies (p o 0.001; Table 4; Fig. 3) than those of similarly exposed osteoarthritis patients. We suggest two possible explanations for the dierence between osteoporotic fracture patients and osteoarthritis patients: (1) fracture patients aected by osteoporosis have lower rates of osteoblastic new bone formation,40 and may therefore incorporate less Gd into remodeling bone at the time of exposure; and (2) bone tissue in osteoporosis undergoes increased bone mineral loss that releases trace metals, including Gd, sequestered in bone tissue. For example, Pb is released from the bone of post-menopausal women, who undergo increased bone mineral loss.34,67 Accordingly, the bone remodeling occurring for many years following Gd exposure could constitute a health hazard particularly for individuals with compromised renal function and/or increased bone resorption. Bone remodeling and metal release may occur at slower rates in normal bone and/or osteoarthritic bone than in osteoporotic bone. Many unknown variables impact the potential toxicity including: Gd-uptake rates in normal, osteoporotic and osteoarthritic bone, residence times of free Gd or Gd chelates, rate of reincorporation into new bone during remodeling, and postdosage clearance mechanisms. Patients with higher rates of bone turnover (e.g. osteoporosis patients, pregnant, lactating and postmenopausal women, and developing fetuses) may have an increased health risk associated with the potential rapid release of toxic elements stored in bone tissue.

from natural sources (e.g. food, soils, water) at a mean of 0.03 nmol/g in cortical tissue (95% CI: 0.023, 0.041 nmol/g) and 0.08 nmol/g in trabecular tissue (95% CI: 0.054, 0.107 nmol/g). We also record the occurrence of an individual with high concentrations of all rare earth elements (REEs), including Gd at a concentration similar to that of a patient exposed to a Gd contrast agent. This proves the necessity of calculating a Gd anomaly from the abundances of adjacent REEs; we normalize Gd anomaly to our control group mean to isolate the amount of Gd in bone attributable to exposure to Gd chelate contrast agents. Gd anomalies in exposed patients range up to 832 with a mean of 280 (95% CI: 124, 460). (2) Gd is retained in bone tissues of exposed patients for longer than 8 years post-exposure. (3) Gd concentrations and anomalies are signicantly lower in the bone tissues of osteoporotic fracture patients exposed to Gd chelates than similarly exposed osteoarthritis patients (p o 0.001) indicating a dierence in the mechanism of metal incorporation according to pathological disease. This could indicate that either: (a) increased rates of bone resorption associated with osteoporosis allow removal and clearance of Gd more rapidly than osteoarthritic bone; or (b) osteoporotic bone incorporates less Gd at the time of exposure because of decreased rates of bone formation and/or metal incorporation. The former would indicate increased risk of endogenous release of Gd into the bloodstream for all patients aected by increased bone resorption (e.g. osteoporosis patients, pregnant, lactating and menopausal women, developing fetuses). (4) Gd incorporation may aect bone quality and health similar to other toxic trace metals. The impact of elevated Gd concentration on bone cellular activity requires further investigation. (5) Given the known toxicity and potential adverse health eects resulting from exposure to free Gd3+, it is essential to investigate and determine the mechanism of incorporation and long-term retention of Gd in bone from Gd-chelate contrast agents.

Conclusions
(1) Bone tissues of patients exposed to Gd-chelate contrast agents have anomalously high Gd concentrations, indicating that Gd is incorporated into bone mineral as a result of contrast imaging and does not completely clear the body after exposure to the contrast agents. While the exact chemical species of Gd was not conclusively determined in this study, these results indicate that Gd chelates may not be stable in vivo and may expose patients to potentially toxic free Gd3+. Clearly further research is required to determine the chemical form of Gd incorporated into bone to fully understand the consequences and potential toxicity of Gd-based contrast enhancement agents. Previous studies have measured Gd at concentrations from 2.8 to 7.5 nmol/g in bones surgically removed 3 days after exposure to high clinical doses of a Gd-chelate. Our results for patients exposed to Gd chelates show similar but higher abundance in bone. ICP-MS sample analysis enables us to establish normal Gd levels in the control group by accurately measuring low concentrations of Gd incorporated into bone
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Acknowledgements
The authors wish to thank the anonymous reviewers whose input substantially improved the manuscript.

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