Slide lab 4,5,6&7

Raed Al-Abbade & tahni abu alteen

Lab 4 **Cross section**

pulp

Dentinal tubules Predentine ODONTOBLASTS Dentine

PDL
layer ome' Granular layer of Tome's Radicular circumpulpal dentine

Cementum Alveolar bone (structurless layer)hyaline layer structurless layer)hyaline note : this picture show the different layers of the root note : Granular layer of tomes lies directly below the hyaline layer

Lab 5

Dentine Predentine

1 2

1 = odontoblastic layer 2 = free-cell zone of Weil 3 = cell-rich zone 4 = pulp core

Demo. : **** Subodontoblastic zone appear after tooth eruption & composed of 2 layers :

1- free-cell zone of weil: (in crown only): result from migration of central cells more than local division of cells . features: anuclear ( not contain cells nuclei ) but contain processes of fibroblasts & odontoblasts , axons and capillaries . 2- cell-rich zone : contain capillaries (Subodontoblastic capillary plexus ), axons (Subodontoblastic neural plexus = plexus of Raschkow ).

2 1
Blood vessels

Predentine Predentine

Dentine

1 = Supraodontoblastic layer 2 = odontoblastic layer

Note : Supraodontoblastic layer : is potential (i.e.: it

is not found in vital but appear after histological preparations as a result from shrinkage of the soft pulp away from the dentine ) space between odontoblastic layer & Predentine.
**It contains 2 things : 1- unsheathed axons . 2- dentritic antigen presenting cells . **it is the 1st level at which the pulp can detect external stimuli.

Note : odontoblastic layer is the effector system of

the pulp & all other cells of the pulp core serve to protect and support it.

Lab 6
Rabitt tooth --------------enamel organ----------bell stage

Dentine Dentine

Dental Dental papilla ERS cells (of Hertwig)

EEE

Demo:
*** ERS cells (of Hertwig): Formed at late bell stage when amelogenesis & dentinogenesis are well advanced . Derived from the cervical loop of the enamel organ Consists of 2 layers IEE +EEE only Not contain stellate reticulum(S.R) or stratum intermedium (S.I) cells. Function : 1- map out the shape of the root & 2- shelves extend from its margins divide the primary apical foramen into secondary apical foramina so determine the number of roots in the tooth .

Enamel Odontoblasts Dental pulp Dentine

Ameloblasts

Epithelial diaphragm

Proliferative zone of D.Follicle

Proliferative zone of D. Pulp

**Note : Epithelial root diaphragm extends apically & it is the only region of the ERS(epithelial root sheath) , that it is continues all the time .

Lab 7
Collagen bundles of PDL dentine dentine

PDL

Acellular cementum

Alveolar bone Blood vessels Cementoblasts Fibroblasts Epithelial rests of malassaez

Note : Epithelial rests of malassaz results from disintegration of the ERS above the root diaphragm.;;;;; it can be distigushed from adjacent fibroblasts by the close packing of their cuboidal cells and their tendency to stain more deeply.

Cellular cementum
Dentine
( Contain cementocytes )

PDL

Osteoblasts Cementocytes Osteocytes blood vessel osteoclasts (in Howship's lacunae)

**Note : Osteocytes dont exhibit obvious canalculi in routine demineralized sections (decalcified sections) ***Note : osteoblasts are specialized fibroblast-like cells of mesenchymal origin.

Cementoclasts

Cellular cementum ( Contain cementocy tes )

Dentine entine