Contents

Quality at mdi Innovative Technologies for Nucleic Acid Purification Plasmid DNA Isolation Kits Selection Chart Process Flow FastLyse pDNA Miniprep Kit pDNA Miniprep Kit Express 96 Miniprep pDNA Kit Quanta Midi Kit Quanta Maxi Kit Express Midi Kit Express Maxi Kit Genomic DNA Isolation Kit Selection Chart Process Flow gDNA Miniprep Kit Plant gDNA Miniprep Kit PCR Purification & Gel Extraction Kits Selection Chart Process Flow PCR Purification Kit Micro PCR Purification Kit Nano PCR Purification Kit Gel Extraction Kits Micro Gel Extraction Kit Nano Gel Extraction Kit Binding membranes for Molecular Biology Membrane Syringe Filters Bottle Top Filters

Page

02 03 04 04 05 - 07 08 09 10 11 12 13 14 15 15 16 17 18 19 19 20-21 22 23 24 25 26 27 28 31 32

Quality at mdi
mdi
Membrane Technologies

Certificate of Quality
FASTLYSE pDNA MINIPREP KIT
Catalog No. Type Lot No.

mdi strives to provide to its customer products and services of highest standards possible, consistently superior and more satisfying than what is available anywhere else. This starts right at the design stage. A careful comparison of user requirements and

The Fastlyse pDNA Miniprep Kit has been manufactured in mdi facility in compliance with ISO 9001 regulations using validated production processes.

: FLPKXXXXXXX0250 : FLPK :

LOT RELEASE CRITERIA Binding Capacity of the column pDNA Yields Culture volume 600l 3.0 ml Yields 7.0 g 20.0 g : 50g

products available from other manufacturers help to distinguish between an acceptable product from an excellent one. The ability to do so has resulted in a driving force that allows us to develop high technology products such as nucleic acid purification kits with unique performance advantages with respect to major user concerns such as yield, purity, consistency (intra lot as well as inter lot ) , processing time, binding capacities and shelf life. The kits have been validated to perform as per pre-determined specifications for a variety of downstream applications.
Advanced Microdevices Pvt. Ltd.
21, Industrial Area, Ambala Cantt, INDIA, Tel: +91-171-2699290/ 2699274 Fax: +91-171-2699221/ 2699008 Website: www.mdimembrane.com Email: mdi @vsnl.com

DNA Purity(A 260 /A280) DNase Free

Plasmid type pUC vectors Copy number - High (500-700) Fragment size - 9 kb Elution volume 50l

: 1.8-2.0 : Yes

VALIDATED FOR Downstream Processing Restriction Digestion Ligation PCR Sequencing Bacterial Transformation Cloning Shelf Life Minimum one year at Room Temperature More than two years at 2-8 C Purification of low copy number plasmids (<10 copies per cell) Purification of small plasmids (2 kb) Consistent intra lot (column to column) and inter lot plasmid DNA recovery CUSTOMER SUPPORT mdi offers its unique interdisciplinary skills to provide solutions to specific problems . Please contact our factory or the local application specialist.

Head of Quality Control Issue Date: 03-May-2010

A well equipped test lab with UV spectrophotometer, fluorometers, UV transilluminator, gel electrophoresis equipment helps validate these unique innovations. ISO 9001: 2008 Certified Quality Management System , careful selection of starting material, validated production processes and testing procedures based on regulatory standards ensure consistently high quality products. The kits undergo stringent quality control tests and are released for sale only after review and approval of data based on compliance to pre-determined test specifications. All mdi products are accompanied with a certificate of quality.

An ISO 9001:2000 Company

High Purity DNA for Automated Sequencing Sequencer: ABI Prism

ABI Prism is the registered trade mark of Applied Biosystems

Page 02 mdi BIOTECH PRODUCT GUIDE

New

Innovative Technologies for Nucleic Acid Purification

mdi introduces a major breakthrough in the field of nucleic acid purification through its latest research. These special kits offer extremely high yields in much reduced process time while effectively addressing other user concerns such as purity, yields, consistency and shelf life.

mdi Fastlyse pDNA Miniprep Kits Unique Performance Advantages

u pDNA isolation in Fastest

Higher Yields with Less Culture Volume

16 14 12 Yield (g) 10 8 6 4 2 0 0.6 Culture Volume (ml) 1.5 mdi Competitor Q

10 minutes.

u No pellet formation and resuspension steps. u with very low culture volumes upto 600l. Works u Very high binding capacity of upto 50g. u Very high yields with low elution volumes. u Very high within lot and inter lot consistency.

mdi Quanta Kits Unique Performance Advantages

u large scale pDNA Isolation in just 30 minutes Fastest u number of operating steps Lesser u Very high binding capacity u No precipitation required for desalting u yields with less culture volume Higher u concentrated, ultrapure pDNA isolation in very low elution volumes Highly u High within lot as well as lot to lot consistency

Higher Yields with Less Culture Volume

250 200 Yield (g) 150 100 50 0 35ml Culture Volume

mdi Competitor Q

100 ml

mdi Nano PCR & Gel Extraction Kits Unique Performance Advantages
u DNA Purification in just 5 minutes Fastest u High DNA Recovery from even low feed quantities u concentrated DNA Highly

High Concentration Recovery

120 100 Recovery % 80 60 40 20 0 61.6 ng 107.6 ng Different Feed Elution Volume : 5l 422.7 ng

in very low elution volumes

u Easy purification of large sized fragments without shearing u for all type of downstream applications Suitable

mdi BIOTECH PRODUCT GUIDE Page 03

Plasmid DNA Isolation Kits

Plasmids are extra chromosomal DNA molecules capable of replicating independently of the chromosomal DNA. This genetic material is ubiquitous in bacteria and because of its special ability to move genes from cell to cell, has become a versatile tool for both researchers and scientists involved in life sciences research. There are several concerns related to researchers such as yields, purity, processing time, binding capacity, consistency and shelf life.

mdi pDNA isolation kits offer many unique advantages with respect to all these issues. The following selection chart will help you choose the most suitable kit for your application.

Selection Chart

pDNA Isolation kits

Purity

Culture volume

Yields

Processing Time

Applications

Small Scale Plasmid DNA Kits

FastLyse pDNA Miniprep Kit

1.8-2.0

600l 5 ml

Upto 25g

10 minutes
? In-Vitro Transcription ? In-Vitro Translation

pDNA Miniprep Kit

1.8-2.0

1 ml -5ml

Upto 25g

30 minutes

? sequencing High quality ? Cloning

Express 96 pDNA Miniprep Kit

1.8-2.0

1 ml -5ml

Upto 25g/well

45 minutes

? Probe Generation ? PCR ? Digestion Restriction ? Bacterial Transformation ? Ligation ? Transfection ? Gene Silencing ? Microinjection ? Library construction

Quanta Midi Kit

Large Scale Plasmid DNA Kits

1.8-2.0

25 35 ml

Upto 250g

20 minutes

Quanta Maxi Kit

1.8-2.0

100-130ml

Upto 1000g

25 minutes

Express Midi Kit

1.8-2.0

50-75 ml

Upto 200g

<1hr

Express Maxi Kit

1.8-2.0

150-250ml

Upto 750g

<1hr

Page 04 mdi BIOTECH PRODUCT GUIDE

Plasmid DNA Purification Kits: Process Flow

Small Scale Plasmid DNA Kits mdi Fastlyse pDNA Miniprep Kit ( upto 25g) mdi pDNA Miniprep Kit ( upto 25g) mdi Express 96 pDNA Miniprep Kit ( upto 25 g/well)

Overnight Bacterial Culture Lyse & Neutralize

Overnight Bacterial Culture Resuspend Lyse Neutralize

Pelleted Bacteria

Resuspend Lyse Neutralize

Bind & Wash

Bind

Express Filter Plate

Filter

Vacuum Wash Elute Express Prep Plate

Bind

Vacuum Dry Wash Ultra Pure pDNA Vacuum

Elute

Elute

Vacuum Microtubes for Ultra Pure pDNA

Ultra Pure pDNA

Tube Rack

mdi BIOTECH PRODUCT GUIDE Page 05

Large Scale Plasmid DNA Kits mdi Quanta Midi Kit ( upto 250g) mdi Quanta Maxi Kit ( upto 1mg)

Pelleted Bacteria

Pelleted Bacteria

Alkaline Lysis

Alkaline Lysis

Clear Lysate

Clear Lysate

By Filtration

By Filtration

Add Binding Buffer

Add Binding Buffer

Bind/Wash

Bind/Wash

Vacuum Pump

Vacuum Pump

Dry

Dry

Elute

Elute

Ultra Pure Plasmid DNA

Ultra Pure Plasmid DNA

Page 06 mdi BIOTECH PRODUCT GUIDE

Large Scale Plasmid DNA Kits mdi Express Midi Kit (upto 200g) mdi Express Maxi Kit (upto 750g)

Bacterial Pellet by centrifugation

Bacterial Pellet by centrifugation

Resuspend Pellet in Solution AL-1

Resuspend Pellet in Solution AL-1

Cell lysis in Solution AL-2 Add buffer AL-3 and wait

Cell lysis in Solution AL-2 Add buffer AL-3 and wait

Filter into Midi column Pass clear lysate by applying vacuum

Filter into Maxi column Pass clear lysate by applying vacuum

Vacuum Pump

Vacuum Pump

Wash and dry again by vacuum

Wash and dry again by vacuum

Elute by vacuum
Vacuum Pump

Elute by vacuum
Vacuum Pump

Add Buffer P Ultra pure concentrated Plasmid DNA

Add Buffer P Ultra pure concentrated Plasmid DNA

Vacuum Pump

Pass through precipitator

Dry and elute by centrifugation

Vacuum Pump

Pass through precipitator

Dry and elute by centrifugation

mdi BIOTECH PRODUCT GUIDE Page 07

FastLyse pDNA Miniprep Kit

The Next Level in pDNA Isolation ( In just 10 minutes)
High Yields
35 30 25 20 15 10 5 0 0.6 1.5 3 4.5 Culture Volume (ml) Elution Volume: 50l

Unique Performance Advantages

u pDNA isolation in Fastest

10 minutes

Yield (g)

u with very low culture volumes (600l) Works u No pellet formation and resuspension steps u Very high binding capacity of upto 50g u Very high yields with low elution volumes u Very high within lot and inter lot consistency

Unique Technology
Direct pDNA isolation from 600l culture without any pelleting and resuspension. A unique soft pellet formation step allows ideal lysis conditions resulting in very
Very High Binding Capacity
Binding Capacity (g) 60 50 40 30 20 10 0 mdi Competitor Q

high yield even with larger culture volumes upto 5ml, helps reduce process time and makes it ideal for plasmid screening.

Applications
u construction Library u Restriction Digestion u Cloning u Ligation u Bacterial Transformation u PCR u Automated Sequencing u Generation Probe u Microinjection

Specifications
pDNA u Binding Capacity : 50 g pDNA u Yield : Upto 25 g pDNA u Size : Upto 25 kb Minimum Culture Volume : 600 l u Elution u Volume : > 30 l Total Time Taken : 10 minutes u

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.8-2.0

Type

XX
Code FLPK

XX

XX

Pack Size
Pack Size 50 250 1000 Code 0050 0250 1000

ORDERING INFORMATION

Type FLPK

Example:

FLPK

XX

XX

XX

0250

Page 08 mdi BIOTECH PRODUCT GUIDE

pDNA Miniprep Kit

(In Less Than 30 Minutes)

Unique Performance Advantages

u High Binding Capacity > 25g u ? High pDNA yields and Purity u Concentrated Yields in low elution volumes ? Highly u ? No Precipitation required for desalting u High within lot and lot to lot consistency

Culture Volume vs. pDNA Recovery and Concentration

pDNA Recovery (g) 30 25 20 15 10 5 0 0 1 2 3 4 5 6 7 Culture Volume(ml) Elution Volume: 50l
pDNA Recovery pDNA Conc.

800 600 400 200 0 pDNA Conc. (ng/l) pDNA Conc.(ng/l)

Applications
u Automated Fluorescent Sequencing u Radioactive Sequencing u Restriction Digestion u Transformation / Transfection u Cloning pDNA Recovery (g) u PCR u Ligation u Generation Probe u Microinjection

Elution volume vs pDNA Recovery and Concentration

pDNA Recovery pDNA Conc. 40 30 20 10 0 30 50 100

1000 800 600 400 200 0 150

Elution volume(l)

Specifications
pDNA Binding Capacity : > 25 g pDNA Yield : Upto 25 g Culture Volume : 1-5 ml Minimum Elution Volume : > 30 l Total Time Taken : 25 minutes
% Recovery

Incubation time vs. Recovery

110 90 70 50 30 10 1 2 3 4 5 10 30 Incubation time (min)
50l 100l

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.8-2.0

Type
Type MIPK Code MIPK

XX

XX

XX

Pack Size

ORDERING INFORMATION

Pack Size 50 250 1000

Code 0050 0250 1000

Example:

MIPK

XX

XX

XX

0250

mdi BIOTECH PRODUCT GUIDE Page 09

Express 96 Miniprep pDNA Kit

(In Less Than 45 Minutes)

Unique Performance Advantages

u Multiple (96) samples processing u Very high binding capacity u No precipitation required for desalting u concentrated, ultrapure pDNA isolation in very low elution volumes Highly

High within lot as well as lot to lot consistency

Applications
u Restriction Enzyme Digestion u Screeining Library u Translation In vitro u Sequencing u Ligation u Transformation / u PCR

Transfection

Specifications
pDNA Binding Capacity/Well : > 25 g pDNA Yield/Well : upto 25 g

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.8-2.0

Culture Volume/Well : 1-5 ml Minimum Elution Volume/Well : 75 l Total Time Taken : 45 minutes

High Consistency: Well to Well

20 16 Yield (g/well) 12 8 4 0 12 24 36 48 Culture Volume: 1.5ml 60 72 84

96

Type

XX Code EMPK

XX

XX

Pack Size Pack Size 4 24 Code 0004 0024

ORDERING INFORMATION

Type EMPK

Example:

EMPK

XX

XX

XX

0024

Page 10 mdi BIOTECH PRODUCT GUIDE

Quanta Midi Kit

(In Just 20 Minutes)

Unique Performance Advantages

u Fastest

Midiprep for pDNA Isolation binding capacity - Upto 400g waiting required for concentration

u Very high

u No gravitational u Simple

filtration to remove cell debris; No centrifugation yields with low culture volume concentrated, ultrapure pDNA isolation in very low elution volumes lot as well as lot to lot consistency

u No precipitation u Higher u Highly

Unique high binding capacity midi spin column

u High within

Unique Technology
Combines the use of a specially designed filtration devices and tube extender to obtain high purity pDNA in just 30 minutes. No desalting is required to obtain ultrapure pDNA yields.

Applications
u Automated u Restriction u Cloning u Transfection u PCR u Ligation u Microinjection u Generation Probe

Vacuum based, no gravitational wetting

Sequencing

Digestion / Transformation

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.8-2.0

Concentrated pDNA, no Isopropanol precipitation

Yield & Binding Capacity
450

Specifications
High Copy Plasmid Binding capacity of membrane ( ds DNA) Recovery Maximum culture volumes Expected yield of plasmid 400 g 80% 25-35 ml 150-250 g Low Copy Plasmid 400 g 80% 50 ml 100-200 g
(g)

400 350 300 250 200 150 100 50 0 mdi Competitor Q Binding Capacity Yield

Type
Type QDPK Code QDPK

XX

XX

XX

Pack Size
Pack Size 25 100 Code 0025 0100

Example:

QDPK

XX

XX

XX

0100

mdi BIOTECH PRODUCT GUIDE Page 11

Quanta Maxi Kit

(In Just 25 Minutes)

Unique Performance Advantages

u Fastest

Maxiprep for pDNA Isolation

u Very high binding capacity - Upto 1200 g u No gravitational waiting u filtration to remove cell debris; No centrifugation Simple u No precipitation required for concentration u yields with low culture volume Higher u concentrated, ultrapure pDNA isolation; in very low elution volumes Highly u High within lot as well as lot to lot consistency

Unique Technology
Combines the use of a specially designed filtration devices and tube extender to obtain high purity pDNA in just 30 minutes. No desalting is required to obtain ultrapure pDNA yields.

Unique high binding capacity spin maxi column

Applications
u Automated u Restriction u Cloning u Transfection u PCR u Ligation u Microinjection u Generation Probe

Sequencing

Digestion / Transformation

Yield & Binding Capacity

1500 1200 900 600 300 0 mdi Competitor Q Yield Binding Capacity

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.8-2.0

Specifications
High Copy Plasmid Binding capacity of membrane ( ds DNA) Recovery Maximum culture volumes Expected yield of plasmid 1200 g 80% 130 ml upto 1000 g Low Copy Plasmid 1200 g 80% 200 ml upto 250 g

(g)

Type

XX
Code
QXPK

XX

XX

Pack Size
Pack Size
10 25

ORDERING INFORMATION

Type
QXPK

Code
0010 0025

Example:

QXPK

XX

XX

XX

0025

Page 12 mdi BIOTECH PRODUCT GUIDE

Express Midi Kit

(Less Than 1 Hour)

Unique Performance Advantages

u Fast pDNA isolation u Very high binding capacity - Upto 350g u High pDNA yields and purity u concentrated yields in very low elution volumes Highly

Applications
u Automated u Restriction u Cloning u Transfection u PCR u Ligation u Microinjection u Generation Probe

Sequencing

Digestion / Transformation

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.8-2.0
Yield and Binding Capacity
400 300 (g) 200 100 0 Yield Binding Capacity

mdi

Competitor Q Elution Volume (ml)

Specifications
High Copy Plasmid Binding capacity of membrane ( ds DNA) Recovery Maximum culture volumes Expected yield of plasmid 350 g 80% 50 ml 100-200 g Low Copy Plasmid 350 g 80% 100 ml 30-100 g

Type
Type Code

XX

XX

XX

Pack Size
Pack Size Code

MDPK

MDPK

25 100

0025 0100

Example:

MDPK

XX

XX

XX

0100

mdi BIOTECH PRODUCT GUIDE Page 13

Express Maxi Kit

(Less Than 1 Hour)

Unique Performance Advantages

u Fast pDNA isolation u Very high binding capacity - Upto 750g u High pDNA yields and purity u concentrated yields in low elution volumes Highly

Applications
u Automated

Sequencing

Filtration of Culture Debris

u Restriction u Cloning

Digestion / Transformation

u Transfection u PCR

DNA Elution by Vacuum

u Ligation u Microinjection u Generation Probe

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.8-2.0

Precipitation

Final Elution of Concentrated DNA

Yield & Binding Capacity

900 750 Binding Capacity 600 (g) 450 300 150 0 Competitor Q mdi Elution Volume (ml) Yield

Specifications
High Copy Plasmid Binding capacity of membrane ( ds DNA) Recovery Maximum culture volumes Expected yield of plasmid 750 g 80% 150 ml 300-750 g Low Copy Plasmid 750 g 80% 250 ml 50-250 g

Type

XX
Code MXPK

XX

XX

Pack Size
Pack Size 10 25 Code 0010 0025

ORDERING INFORMATION

Type MXPK

Example:

MXPK

XX

XX

XX

0025

Page 14 mdi BIOTECH PRODUCT GUIDE

Genomic DNA Isolation Kits

gDNA isolation is required for a variety of applications such as genomic sequencing, transfection and microinjection. Scientists are naturally concerned not only about the quality and yields, but also the shelf life, consistency and processing time. mdi offers well characterised and validated Genomic DNA Miniprep Kits which reproducibly provide ultrapure gDNA from a wide variety of samples such as plant tissues, mammalian tissue, blood, bacteria and cultured cells.

Selection Chart

Sample Types Mammalian Tissue - Liver - Kidney - Lung - Spleen - Mouse Tail Animal Blood

Purity

Amount of Starting Material

Yields

Processing Time

Applications

? In-Vitro Transcription ? In-Vitro Translation

1.8-2.0

25mg

10 -30g

< 1hr

? Sequencing Automated ? Cloning ? Blotting Southern

gDNA Miniprep Kit

1.8-2.0

200l

Upto 12g

<1hr

? AFLP ? RFLP

Bacteria - Gram Positive - Gram Negative 1.8-2.0 2 x 10 cells

9

Upto 22g

45 minutes

? RAPD ? PCR

Cultured Cells

1.8-2.0

5 x 106 cells

Upto 25g

<1hr

? Digestion Restriction ? Transformation

Plant gDNA Miniprep Kit

? Ligation ? Transfection

100 mg wet weight Plant Tissue 1.7-1.9 20 mg dry weight

? Microsatellite analysis ? RT-PCR

Upto 30g

30 minutes

? SNP Genotyping

mdi BIOTECH PRODUCT GUIDE Page 15

Genomic DNA Isolation Kits: Process Flow

gDNA Miniprep Kits mdi Genomic DNA Miniprep Kit mdi Plant gDNA Miniprep Kit

Sample

Plant Tissue Grind Lyse Precipitate

Lyse

Bind

Centrifuge through mdi Shredder

Wash 2X

Add Binding Buffer and transfer to mdi plant mini spin column

Bind DNA Dry with open lid

Wash 2X Elute

Elute Ultra Pure gDNA

Ultra Pure gDNA

Page 16 mdi BIOTECH PRODUCT GUIDE

gDNA Miniprep Kit

( for Mammalian Tissue, Blood, Bacterial Cells and Cultured Cells )

Unique Performance Advantages

u high binding capacity - Upto 50g Very u Higher yields Yield (g) u No precipitation step for high purity gDNA. u Suitable for all type of downstream applications

Highly Concentrated Yields

Second Elution First Elution

60 50 40 30 20 10 0
mdi

Applications
u PCR u Southern Blotting u Analysis RAPD u Analysis AFLP u Analysis RFLP u In- Vitro Transcription u Restriction Digestion

Competitor Q

Elution Volume: 200l/Elution

op

th

aq

iD

rm

rm

ke

ar

ol

u RT - PCR u Silencing Gene u Microinjection u Generation Probe

Th e

Th e

Ec

Specifications
Maximum Amount of Tissue : 25 mg Maximum bacterial cells : 2 x 109 Maximum volume of blood sample : 200l Maximum amount of cultured cells : 5 x 106 Capacity of column reservoir : 700l Binding capacity of membrane (ds DNA) : 50 g Recovery : 80% Minimum elution volume : 200l

Agarose gel analysis of gDNA from different bacteria purified with mdi gDNA Miniprep Kit . M: lambda-HindIII

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.8-2.0

Type
Type CTGK Code CTGK

XX

XX

XX

Pack Size

ar

Pack Size 50 250 1000

Code 0050 0250 1000

Example:

CTGK

XX

XX

XX

0250

mdi BIOTECH PRODUCT GUIDE Page 17

ke

u Microsatellite Analysis

us

us

h5 r

u Genotyping SNP

er

ua

tic

u Transfection

hi lu s

u Transformation

us

ORDERING INFORMATION

Plant gDNA Miniprep Kit

Highly Concentrated Yields
15 12 Yield (g) 9 6 3 0 mdi Competitor Q ( Tomato Leaves)

Unique Performance Advantages

u Very high binding capacity - Upto 50g u yields with different plant tissue Higher u No precipitation step for high purity gDNA

Suitable for all type of downstream applications

Applications
u PCR u Southern Blotting u Analysis RAPD u Analysis AFLP u Analysis RFLP

gDNA: Yield
25 20 Yield (g) 15 10 5 0 Tomato Potato Spinach Wheat Chick pea Different Samples Rice Fungus

u In- Vitro Transcription u Restriction Digestion u Transformation u Transfection u Genotyping SNP u Microsatellite Analysis u RT - PCR u Silencing Gene u Microinjection u Generation Probe

Specifications
Maximum Amount of Starting Material : 100 mg wet weight, 20 mg dry weight Capacity of column Reservoir : 700l Binding capacity of membrane (ds DNA) : 50 g Recovery : 80% Elution volume : 100l

Purity
Ultrapure Plasmid DNA: A260 / A280 = 1.7-1.9

Type

XX
Code PTGK

XX

XX

Pack Size
Pack Size
50 250 1000

ORDERING INFORMATION

Type PTGK

Code
0050 0250 1000

Example:

PTGK

XX

XX

XX

1000

Page 18 mdi BIOTECH PRODUCT GUIDE

PCR Purification and Gel Extraction Kits

mdi offers PCR Purification Kits for efficient removal of contaminants such as primers, enzymes and salts and Gel Extraction Kits to purify very small quantities of even large fragment size DNA from upto 400mg of gel slices. The following selection chart will help you choose the most suitable kit for your experiment.

Selection Chart

DNA Isolation Kits

Purity

DNA Quantity

Recovered DNA Fragment

Elution Volume

Applications

? Transfection

PCR Purification Kits

PCR Purification Kit

1.8-2.0

5-10g

100bp -10kb

30l

? Transcription In-Vitro ? Translation In-Vitro ? High quality sequencing

Micro PCR Purification Kit

1.8-2.0

1-2g

70bp -4kb

10l
? Microarray Analysis ? Cloning

Nano PCR Purification Kit

1.8-2.0

upto 400ng

70bp -4kb

5l

? PCR ? Gene Silencing

Gel Extraction Kit

Gel Extraction Kits

1.8-2.0

5-10g

70bp -10kb

30l

? Probe Generation ? Microinjection ? Restriction Digestion

Micro Gel Extraction Kit

1.8-2.0

1-2g

70bp -4kb

10l

? Demanding enzymatic

modifications
?Construction Library

Nano Gel Extraction Kit

1.8-2.0

upto 400ng

70bp -4kb

5l

? Transformation Bacterial ? Ligation

mdi BIOTECH PRODUCT GUIDE Page 19

PCR Purification Kits

mdi PCR Purification Kit (5-10g) mdi Micro PCR Purification Kit (1-2g) mdi Nano PCR Purification Kit (upto 400ng)

PCR reaction

PCR Reaction

PCR Reaction

Add 5 volumes of buffer PB

Add 5 volumes of buffer MPB

Add 5 volumes of buffer NPB

Bind

Bind

Bind

Wash

Wash

Wash

Dry

Dry

Dry

Elute

Elute

Elute

Ultra Pure DNA Fragment

Ultra Pure DNA Fragment

Ultra Pure DNA Fragment

Page 20 mdi BIOTECH PRODUCT GUIDE

Gel Extraction Kits

mdi Gel Extraction Kit (5-10g) mdi Micro Gel Extraction Kit (1-2g) mdi Nano Gel Extraction Kit (upto 400ng)

Solubilized Gel Slice

Solubilized Gel Slice

Solubilized Gel Slice

Bind

Bind

Bind

Wash

Wash

Wash

Dry

Dry

Dry

Elute

Elute

Elute

Ultra Pure DNA Fragment

Ultra Pure DNA Fragment

Ultra Pure DNA Fragment

mdi BIOTECH PRODUCT GUIDE Page 21

PCR Purification Kit

(5-10g)

Unique Performance Advantages

u Easy purification of large sized fragments without shearing u No precipitation step for high purity DNA u concentrated DNA yields in low elution volume Highly

Applications
u Transfection u Transcription In-Vitro u Translation In-Vitro u High quality sequencing u Microarray Analysis u Cloning u PCR u Gene Silencing u Generation Probe

u Microinjection u Restriction Digestion u Demanding enzymatic modifications u Construction Library

1000 bp 200 bp Primer

u Bacterial Transformation u Ligation

Complete primer removal using mdi PCR Purification Kit a: Before purification b: After purification M: Marker

Specifications
Capacity of column reservoir : 800l Binding capacity of membrane (ds DNA) : 10 g Recovery : 90-95% Recovered DNA fragment : (100 bp -10 kb) Minimum elution volume : 30 l Total eluate volume : 28 l

Purity
Ultrapure Plasmid DNA: A260 / A280 =1.8-2.0

Type

XX
Code SPCK

XX

XX

Pack Size
Pack Size 50 250 1000 Code 0050 0250 1000

ORDERING INFORMATION

Type SPCK

Example:

SPCK

XX

XX

XX

0250

Page 22 mdi BIOTECH PRODUCT GUIDE

Micro PCR Purification Kit

(1-2g)

Unique Performance Advantages

u Easy purification of large sized fragments without shearing u No precipitation step for high purity DNA

High Concentration Recovery

120 100 % Recovery 80 60 40 20 0 mdi Competitor Q Feed: 1g

u concentrated DNA yields in very low elution volume Highly

Applications
u Transfection u Transcription In-Vitro u Translation In-Vitro u High quality sequencing u Microarray Analysis u Cloning u PCR u Gene Silencing u Generation Probe u Microinjection u Restriction Digestion u Demanding enzymatic modifications u Construction Library u Bacterial Transformation u Ligation

Specifications
Capacity of column reservoir : 800l Binding capacity of membrane (ds DNA) : 5 g Recovery : 80% Recovered DNA fragment : (70 bp - 4 kb) Minimum elution volume : 10 l Total eluate volume : 9 l

Purity
Ultrapure Plasmid DNA: A260 / A280 =1.8-2.0

Type
Type MPCK Code MPCK

XX

XX

XX

Pack Size

ORDERING INFORMATION

Pack Size 50 250 1000

Code 0050 0250 1000

Example:

MPCK

XX

XX

XX

0250

mdi BIOTECH PRODUCT GUIDE Page 23

Nano PCR Purification Kit

(upto 400ng))
High Concentration Recovery
120 100 % Recovery 80 60 40 20 0 61.6 ng 107.6 ng Feed Elution Volume : 5l 422.7 ng

Unique Performance Advantages

u with very small DNA quantities Works u DNA Purification in just 5 minutes Fastest u High DNA Recovery and yield from even low feed quantities u concentrated DNA in very low elution volume Highly u Easy purification of large sized fragments without shearing

Applications
u Transfection u Transcription In-Vitro u Translation In-Vitro u High quality sequencing u Microarray Analysis u Cloning u PCR u Gene Silencing u Generation Probe u Microinjection u Restriction Digestion u Demanding enzymatic modifications u Construction Library u Bacterial Transformation u Ligation

Specifications
Capacity of column reservoir : 800l Binding capacity of membrane (ds DNA) : 5 g Recovery : 80-85% Recovered DNA fragment : (70 bp-4 kb) Minimum elution volume : 5l Total eluate volume : 4l

Purity
Ultrapure Plasmid DNA: A260 / A280 =1.8-2.0

Type

XX
Code NPCK

XX

XX

Pack Size
Pack Size 50 250 1000 Code 0050 0250 1000

ORDERING INFORMATION

Type NPCK

Example:

NPCK

XX

XX

XX

0250

Page 24 mdi BIOTECH PRODUCT GUIDE

Gel Extraction Kit

(5-10g)

Unique Performance Advantages

u Easy purification of large sized fragments without shearing u No precipitation step for high purity DNA u concentrated DNA yields in low elution volume Highly

Gel weight vs. % Recovery

100

80 % Recovery 60 40 20 0 50 100 150 200 250 300 350 400 Gel Weight (mg) Elution Volume (30 l)

Applications
u Transfection u Transcription In-Vitro u Translation In-Vitro u High quality sequencing u Microarray Analysis u Cloning u PCR u Gene Silencing u Generation Probe u Microinjection u Restriction Digestion u Demanding enzymatic modifications u Construction Library u Bacterial Transformation u Ligation

1000 bp

Specifications
Capacity of column reservoir : 800l Maximum weight of gel slice : 400mg Binding capacity of membrane (ds DNA) : 10 g Recovery : 70-80 % Recovered DNA fragment : (70 bp-10 kb) Minimum elution volume : 30 l

200 bp

High recovery using mdi Gel Extraction Kit B: Before extraction 1-2: After extraction M: Marker

Purity
Ultrapure Plasmid DNA: A260 / A280 =1.8-2.0

Type
Type SGEK Code SGEK

XX

XX

XX

Pack Size

ORDERING INFORMATION

Pack Size 50 250 1000

Code 0050 0250 1000

Example:

SGEK

XX

XX

XX

1000

mdi BIOTECH PRODUCT GUIDE Page 25

Micro Gel Extraction Kit

(1-2g)
Highly Concentrated Recovery
90 75 % Recovery 60 45 30 15 0 mdi Feed: 1g Competitor Q

Unique Performance Advantages

u Easy purification of large sized fragments without shearing u No precipitation step for high purity DNA u concentrated DNA yields in very low elution volume Highly

Applications
u Transfection u Transcription In-Vitro u Translation In-Vitro u High quality sequencing u Microarray Analysis u Cloning u PCR u Gene Silencing u Generation Probe u Microinjection u Restriction Digestion u Demanding enzymatic modifications u Construction Library u Bacterial Transformation u Ligation

Specifications
Capacity of column reservoir : 800l Maximum weight of gel slice : 400mg Binding capacity of membrane (ds DNA) : 5 g Recovery : 80 % Recovered DNA fragment : (70bp-4 kb) Minimum elution volume : 10 l

Purity
Ultrapure Plasmid DNA: A260 / A280 =1.8-2.0

Type

XX
Code MGEK

XX

XX

Pack Size
Pack Size 50 250 1000 Code 0050 0250 1000

ORDERING INFORMATION

Type MGEK

Example:

MGEK

XX

XX

XX

0250

Page 26 mdi BIOTECH PRODUCT GUIDE

Nano Gel Extraction Kit

(upto 400ng)

Unique Performance Advantages

u Fastest

High Concentration Recovery

120 100 % Recovery 80 60 40 20

DNA Purification DNA binding capacity Recovery and yield from even low feed quantities of large sized fragments without shearing

u Very high u High DNA u Highly

concentrated DNA ; in very low elution volumes

u Easy purification

Applications
u Transfection u Transcription In-Vitro u Translation In-Vitro u High quality sequencing u Microarray Analysis u Cloning u PCR u Gene Silencing u Generation Probe u Microinjection u Restriction Digestion u Demanding enzymatic modifications u Construction Library u Bacterial Transformation u Ligation

0 54.6 ng 128.3 ng Feed Elution Volume : 5 l 392.8 ng

Specifications
Capacity of column reservoir : 800l Binding capacity of membrane (ds DNA) : 5g Recovery : 80-85 % Recovered DNA fragment : (70bp-4 kb) Minimum elution volume : 5l Total eluate volume : 4l

Purity
Ultrapure Plasmid DNA: A260 / A280 =1.8-2.0

Type
Type NGEK Code NGEK

XX

XX

XX

Pack Size

ORDERING INFORMATION

Pack Size 50 250 1000

Code 0050 0250 1000

Example:

NGEK

XX

XX

XX

0250

mdi BIOTECH PRODUCT GUIDE Page 27

Binding Membranes for Molecular Biology

mdi binding membranes are uniform, paper thin, white plastic supports, having specially designed porous structures and binding sites suitable for transfer and hybridization of biological molecules. mdi offers a wide range of binding membranes viz. Nitrocellulose, Nylon-66, and PVDF, exhibiting a range of properties to suit various applications.

Special features
u High binding capacities of the transferred molecules u Good wettabilty for Nitrocellulose and Nylon-66 membranes umembranes PVDF

are hydrophobic

u to retain the molecules without affecting its biological activity Ability u Chemical

compatibility and mechanical durability

u to be blocked by simple procedures Ability u High signal to noise ratio

For special applications, mdi offers internally supported binding membranes which
Protein Binding
150 100 50 0 SCN Protein binding capacities of SCN membranes with BSA

exhibit very high mechanical strength.

0.2m 0.45m

Nitrocellulose Membrane Type - SCN and SCNJ

SCN Pure nitrocellulose membrane produced specially for life sciences applications. SCNJ Internally supported to offer superior handleability.

(g/cm2)

120 105

Characteristics
u High binding capacities for proteins and nucleic acid molecules u Minimum u Uniform u Can be

background: High signal to noise ratio

and easy wettability bind common protein stains with colorimetric, radiolabelled, chemiluminescent, fluorescent,

blocked by normal blocking methods

u Does not

u Compatible

and staining detection methods

Applications
u blotting Protein u slot blots Dot and u acid dot/slot blots Nucleic u Colony/plaque lifts u immunoassays Enzyme

Packaging
Sealed in aluminum bags to maintain hydrophilicity and high protein binding on prolonged storage. The separators are of pure polyester film to avoid any contamination of the membrane from the separator.

Page 28 mdi BIOTECH PRODUCT GUIDE

PVDF Membrane - Type SVF

mdi Polyvinylidene fluoride (PVDF) membrane is a naturally hydrophobic support matrix which offers much higher protein binding capacities than that of Nitrocellulose membrane and also binds difficult to bind proteins such as
(g/cm )
2

Protein Binding
250 200 150 100
105 190 240

SVF SCN

glycoproteins.

Characteristics
u Very high u Minimal u Remains

120

binding capacities

50 0 0.45 Pore Size (m) Protein binding capacities of SVF and SCN membranes with BSA 0.2

background: High signal to noise ratio flexible and non-brittle after processing resistant to harsh reagents making it a convenient matrix for

u Chemically

protein sequencing
u Higher

strength than pure nitrocellulose with all types of detection methods

u Compatible

Applications
u Ideal support matrix for protein sequencing u performance, reproducible western blotting For high u For protein staining, glycolipid

detection and immunoblotting

Nylon-66 Membrane Type - SNNP and SNNPZ

SNNP: Internally supported Nylon-66 membranes SNNPZ: Positively charged for enhanced binding of negatively charged molecules

Characteristics
u Very high

binding capacities for nucleic acid molecules cross linkable resistant; tolerant to alkali fixation

u Easy wettabilty u Ultraviolet

u Chemically

Applications
u Nucleic

acid transfers

u Dots/slot blots u Colony/plaque lifts u Multiple reprobing

Type
Type SCN SCNJ SVF SNNP SNNPZ Code SCNX SCNJ SVFX SNNP SNPZ

Size
Dia 82mm 90mm 137mm 142mm 80mm x 100mm 150 x 150mm 200 x 200mm 300 x 300mm 3M X 300mm 3M x 240mm 3M x 100mm Code 13 14 20 16 88 87 86 85 84 83 81

Pore Size
Pore Size 0.2m 0.45m Code 01 02

XX

XX

Sterile/ Non Sterile

Non Sterile Code 1

Pack Size
Pack Size 25 50 Roll Code 11 03 01

ORDERING INFORMATION

* SVF, SNNP and SNNPZ are available in a maximum width of 240 mm ** SCNJ is available in a maximum width of 100mm Example: SCNJ 13 02 XX XX 1 03

mdi BIOTECH PRODUCT GUIDE Page 29

Selection Chart
This selection chart highlights the suitability of various mdi membranes for different applications based on their properties.

Membrane Type

SCN

SCNJ

SNNP

SNNPZ

SVF

Biomolecules Nucleic Acids Proteins Transfer Method Dot Blot Colony or Plaque lift Electrotransfer Capillary Blot Vacuum Blot Alkaline Transfer Molecule Fixation Baking Drying UV Crosslinking Alkali Fixation Molecule Removal Detection Method Colorimetric Radiolabelled Luminesence Fluorescence Staining Reprobing Once Multiple HR R R* P NR = = = = = NR NR Highly Recommended Recommended Recommended for Proteins only Possible Not Recommended R R R R R R R R HR R R R R HR R R R R R R P P P R R P P P R R R R R R R P NR NR R R P NR R R R HR R NR R R HR R NR NR R R R R R HR R* R R NR R HR R* R R NR R R HR R R R R R HR R R R R NR HR R R R R HR R HR HR R HR R NR R

Page 30 mdi BIOTECH PRODUCT GUIDE

Pre-Sterilized Polyethersulfone Membrane Syringe Filters

SYPL-S Pre-sterilized syringe filters are available in pore sizes of 0.2m and 0.45m and several diameters to suit various applications for filtration in the laboratory.

Special Types Available

SY25KG-S This special syringe filter houses a microglassfiber pre-filter along with the Polyethersulfone membrane filter and is ideal for filtering difficult to filter solutions. IKG-S This 50mm Polyethersulfone syringe filter with 1/4 stepped hose barb connections incorporates large filtration area and microglassfiber pre-filter for larger volume filtration. These filters can be used with 50ml syringe to filter volumes up to 500ml.

Special Feature
Extremely low protein binding exhibited by mdi syringe filters results in very high product recovery.

Protein Recovery

Specifications
Pore Size Diameter EFA* Hold-Up Volume Retention Efficiency 4mm 0.07cm <5l 0.2m, 0.45m 13mm 0.8cm <20l 25mm 4.15cm <50l 50mm 18cm

100

BSA

99.9%

98%

50

<300l

SYPL

Competition

0.2m: LRV >7 for Br. diminuta 0.45m: LRV >7 for Sr. marcescens

Type Type
SYPL SYKG IKG Code SYPL SYKG IKGX

Size
Dia 4mm 13mm 25mm

Pore Size
Code Pore Size Code 01 03 06 0.2m 0.45m 01 02

Inlet/Outlet
Code Female Luer Lock Male Luer Slip 1/4 SHB* M N B

Bell
Size With Bell B

Sterile/ Non Sterile

Code Non Sterile Sterile 1 2

Pack Size

ORDERING INFORMATION

Pack Size Code 100 12 04 08

Without Bell X

Note: only IKG-S filters are available with bell Example:

SYKG

06

01

MN

04

mdi BIOTECH PRODUCT GUIDE Page 31

Vacufil-S: Pre-Sterilized Bottle Top Vacuum Filters

mdi Pre-Sterilized Bottle Top Vacuum Filtration units with an extra large 75mm diameter, low protein binding Polyethersulfone membrane are the best option for filtration of biologicals like sera and culture media, and other proteinaceous solutions. Vacufil filters have a hydrophobic filter in the vacuum arm to prevent passage of filtrate to the pump. These filters screw perfectly on to vacuum safe bottles with 45mm neck size.

Features
u Low protein binding u Extra large filter area u High flow rates uIntegrity tested 100% u No elastomers or adhesive used in sealing u Non-toxic materials of construction

Specifications
Pore Size : 0.2m, 0.45m Diameter : 75mm Connection : 45mm (Screw cap neck) Hold-Up Volume : <3ml Retention Efficiency : 0.2m: LRV>7 for Br. diminuta

Type

Size
Code VFPX Dia 75mm Code 11

Pore Size
Pore Size Code 0.2m 0.45m 01 02

XX

XX

Sterile/ Non Sterile

Code Sterile 2

Pack Size
Pack Size 12 24 Code 08 12

ORDERING INFORMATION

Type Vacufil

Example:

VFPX

11

01

XX

XX

12

Page 32 mdi BIOTECH PRODUCT GUIDE