CURRICULUM VITAE Richard A.

Juneau EDUCATION: 2006-Present: PhD, Microbiology and Immunology, Degree expected Summer 2011, Wa ke Forest University Graduate School of Arts and Sciences, Winston Salem, NC 2001-2005: LA B.S. Biological Sciences, Louisiana State University, Baton Rouge,

RESEARCH EXPERIENCE: 2006-Present: Graduate Research, Wake Forest University School of Medicine, Depa rtment of Microbiology and Immunology Advisor: William E. Swords, PhD Research foci include Nontypeable Haemophilus influenzae (NTHi)-induced otitis m edia through utilization of an in vivo chinchilla model, in vitro and in vivo bi ofilm formation, as well as study of the induction and antimicrobial effects of neutrophil extracellular traps using primary human or murine cells or immortaliz ed cell lines. Academic requirements include regular data presentation within t he departments bacteriology focus group, as well as a yearly formal seminar for the Department of Microbiology and Immunology. 2005-2005: Intern, NuPotential, Inc. Louisiana Emerging Technology Center, Bat on Rouge, LA Mentor and Senior Scientist: Rachel Power, PhD Responsibilities: In a research atmosphere focused on cellular reprogramming and epigenetics, I developed skills in tissue culture, light microscopy, polymerase chain reaction, and general lab maintenance. 2003-2005: Undergraduate Student Worker, Louisiana State University, Department of Biological Sciences, Baton Rouge, LA Supervisor: Hollie Hale-Donze, PhD Responsibilities: In a laboratory studying macrophage responses to microsporidi a, I developed skills in isolation and culture of peripheral blood mononuclear c ells, in vitro cellular infection, immunohistochemical staining, and general lab maintenance. 2003-2004: Student Worker, Pennington Biomedical Research Center, Baton Rouge, LA Supervisors: Randall Mynatt, PhD and Rachel Power, PhD Responsibilities: In the transgenics core, I bred, weaned, and genotyped mouse c olonies, organized and updated pedigrees, and performed general lab maintenance. PUBLICATIONS: Mathew S. Byrd, Bing Pang, Wenzhou Hong, Elizabeth A. Waligora, Richard A. Junea u, James Turner, Chelsie E. Armbruster, Kristin E. D. Weimer, Kyle Murrah, Haipi ng Lu, April Sprinkle, Mathew R. Parsek, Nancy D. Kock, Daniel J. Wozniak, W. Ed ward Swords. Direct evaluation of Pseudomonas aeruginosa biofilm mediators in a chronic infection model. Infection and Immunity. Manuscript submitted. K.E.D. Weimer, R.A. Juneau, K.A. Murrah, B. Pang, C.E. Armbruster, S.H. Richards on, W.E. Swords. Divergent mechanism for passive pneumococcal resistance to *-l actam antibiotics in the presence of Haemophilus influenzae. J Infect Dis, 203( 4): 549-555. 2011.

Richard A. Juneau, Bing Pang, Kristin E. D. Weimer, Chelsie E. Armbruster, W. Ed ward Swords. Nontypeable Haemophilus influenzae initiates formation of neutroph il extracellular traps. Infection and Immunity, 79(1): 431-438. 2011. Kristin E. D. Weimer, Chelsie E. Armbruster, Richard A. Juneau, Wenzhou Hong, Bi ng Pang, W. Edward Swords. Coinfection with Haemophilus influenzae Promotes Pne umococcal Biofilm Formation during Experimental Otitis Media and Impedes the Pro gression of Pneumococcal Disease. J Infects Dis, 202 (7): 1068-1075. 2010. Chelsie E. Armbruster, Wenzhou Hong, Bing Pang, Kristin E. D. Weimer, Richard A. Juneau, James Turner, W. Edward Swords. Indirect Pathogenicity of Haemophilus influenzae and Moraxella catarrhalis in Polymicrobial Otitis Media Occurs via In terspecies Quorum Signaling. mBio. July 6, 2010. Chelsie E. Armbruster, Wenzhou Hong, Bing Pang, Kristin E. Dew, Richard A. Junea u, Matthew S. Byrd, Cherton F. Love, Nancy D. Kock, W. Edward Swords. LuxS Prom otes Biofilm Maturation and Persistence of Nontypeable Haemophilus influenzae In Vivo via Modulation of Lipooligosaccharides on the Bacterial Surface. Infectio n and Immunity. 77(9). September 2009. Wenzhou Hong, Richard A. Juneau, Bing Pang, W. Edward Swords. Survival of Bacte rial Biofilms within Neutrophil Extracellular Traps Promotes Nontypealbe Haemoph ilus influenzae Persistence in the Chinchilla Model for Otitis Media. Journal o f Innate Immunity. 1:215-224, April 2009. Authors contributed equal work. Jeffrey Fischer, Diana Tran, Richard A. Juneau, Hollie Hale-Donze. Kinetics of Encephalitozoon spp. infection of human macrophages. Journal of Parasitology. 94(1): 169-175, February 2008. FORMAL PRESENTATIONS: Richard A. Juneau. 2011. Nontypeable Haemophilus influenzae initiates and surv ives within neutrophil extracellular traps. Oral Presentation. Mid-Atlantic Mi crobial Pathogenesis Meeting; Wintergreen, VA. Richard A. Juneau, Bing Pang, Kristin E.D. Weimer, Chelsie E. Armbruster, W.Edwa rd Swords. 2010. Nontypeable Haemophilus influenzae initiates formation of neu trophil extracellular traps. Poster presentation. American Society for Microb iology General Meeting; San Diego, CA. *Richard A. Juneau, Wenzhou Hong, Bing Pang, W. Edward Swords. 2008. Survival of Bacterial Biofilms within Neutrophil Extracellular Traps Promotes Nontypealbe Haemophilus influenzae Persistence in the Chinchilla Model for Otitis Media. Po ster presentation. International Pasteurellaceae Society Meeting; Sorrento, I taly. * The same abstract was also presented at Graduate Student Research Day, Wake Fo rest University, 2008. Richard A. Juneau. 2008. Neutrophil Extracellular Traps: Induction and Persis tence Mechanisms of Nontypeable Haemophilus influenzae. Oral presentation. Fal l Research Symposium, Wake Forest University Department of Microbiology and Immu nology TEACHING: 2010: Student Lecturer, Department of Microbiology and Immunology, Invited to present a bacteriology course lecture to first year graduate students overviewing evasive mechanisms of extracellular bacteria.

PROFESSIONAL AFFILIATIONS: 2008-Present: American Society for Microbiology