SPECTRO 2000 RS & SPECTRO 2000 RSP OPERATING MANUAL

2 Thank You Very Much For Your Purchase Of This High Performance Instrument. This Manual Will Introduce The Automatic SPECTRO 2000 RS & SPECTRO 2000 RSP Microprocessor Spectrophotometer. Before Use, Please Read This Manual Carefully. IMPORTANT This Spectrophotometer Is Thoroughly Checked And Inspected Before Shipment. The Voltage Power Switch At The Back Of The Spectro Has Been Set for 110V. 60Hz. (U.S.A. Voltage Power). If The Voltage Power In Your Country Is 220V. 50Hz., You Can Convert It, By Switching The Voltage Switch At The Back Of The Spectro To 220V.. Before Any Action Or Use, Check The Spectro & Switch It to 220V., If Necessary. Use This Procedure To Avoid Any Damage To The Instrument. TABLE OF CONTENTS Page:

Table Of Contents: Specifications:

2 3 4 5 6 7 8 12 13 14 15 16 17

Main Application & Installation Abbreviations: Spectro 2000 RS & Spectro 2000 RSP Front Panel: Instructions To Use: Operating Manual: Example A: Example B: Example C: Example D: Example E: Example F:

3 SPECIFICATIONS Wavelength: BandPass: Stray Light: Wavelength Repeatability: Wavelength Accuracy: Light Source: 325 - 1100nm 6nm 0.1%T At 340nm And 400nm + 0.5nm +1nm Tungsten Halogen Lamp for 1000 hours 1200 lines / 1mm Two Lines RS232 110V. 60Hz. or 220V. 50Hz. 1999 2000 2000 16 x 14 x 9 inches 20 lbs.

Grating Mirror: Data: Link To Computer & Printer Power: Transmittance T%: Absorption: Concentration: Dimension & Weight:

MAIN APPLICATIONS

The Spectro 2000 RS Spectrophotometer Is A High Technology Instrument, That Is Used For Qualitative & Quantitatif Analysis To Measure Absorption (A), Percent Transmittance (T), Or Concentration (C) with Wavelength Range of 325 -1100nm. Spectro 2000 RS Is Best Suited For Various Fields Such As: Clinical Examination, Biochemistry, Agricultural Research, Petrochemistry, Chemistry, Environmental Protection, And Quality Control. Spectro 2000 RS With Soft Key Has A Microprocessor With Memory, And Storage Functions. It Provides Power Shut-Off Protection. ( It Will Be Shut Off Automatically When It Is Not In Use) The Spectro Is Also Equipped With Interface RS232 To Link To An IBM Compatible Computer And Printer. The Spectro 2000 RSP Has A Built In Printer.

INSTALLATION AND REQUIREMENTS 1. This Instrument Should Be Installed In A Low Corrosive, Fume Free Environment, With A Firm And Stable Table Or Surface. 2. Before Plugging In The Unit, The Power Should Be Checked At The Back Of The Spectro 2000 RS, & Spectro 2000 RSP. The Spectro Voltage Power Switch Should Be Checked To See If It Has To Be Converted According To The Lab. Power. (110V. or 220V. ) To Prevent Damage From The High Tech Instrument, Please Follow This Procedure. WARNING!!! If The Voltage Power In Your Country Is 220V. 50Hz., And It Has Not Been Checked Or Converted To 220V., Do Not Operate. If It Is Operated With The Wrong Voltage, The Spectro Will Be Damaged.

ABBREVIATIONS

A T C R S1 S2 S3 ESC CHA FACTOR ACC Lamp Energy EXT Print INT Print INIT CONC

= = = = = = = = = = = = = = =

Absorption Transmittance Concentration Reference Sample 1 Sample 2 Sample 3 Escape Change Function & Factor Accept Need Lamp Adjustment External Printer Internal Printer Initial Concentration

Front Panel of Spectro 2000RS and Spectro 2000RSP

7 INSTRUCTIONS TO USE 1. First Check The Voltage Switch At The Back Of The Spectro 2000 RS & Spectro 2000 RSP To be Converted To The Lab. Power, Then Proceed To Plug In The Power. 2. Turn On Power Switch 3. The Wording On The Display Will Show Please Wait (Instrument Is Warming) The Spectro Will Make Noise During This Process While It Is Being Warmed Up. 4. After The Unit Has Warmed Up, (The Motor Sound has Ended) The Display Will Show: 550nm Wavelength, A or T: ( Absorption or Transmittance), And R or S1 - S2 - S3 Sample Position. 5. Now You Can Command The Instrument By The 10 Soft Keys

8 10 KEYS 12345678910ATC To Choose Absorbance (A), Transmittance (T), Or Concentration (C) ABC Adjusting 0A And 100%T FACTOR = Function Or Factor Or Menu Print Move Cuvette Forward Move Cuvette Backward Enter = Enter Data Decrease Wavelength Increase Wavelength ESC = Escape

OPERATING MANUAL SPECTRO 2000 RS SPECTRO 2000 RSP The Spectro 2000 RS And Spectro 2000 RSP Are High Technology Instruments For Different Analytical Tests Of Spectrophotometry That Can Be Used For Chemistry, Biochemistry, Water Analysis, Environmental Research, & Medical Diagnostic Tests. The Spectro 2000 And Spectro 2000 RSP Is A Microprocessor System With A Memory And Storage Function. It Provides Power Shut Off Protection And Is Equipped With Standard Interface RS232 For Connection To An IBM Compatible Computer & External Printer. Spectro 2000 RS & Spectro 2000 RSP Can Measure Absorption, Transmittance, And Concentration For 4 Different Samples At One Time. There Are 10 Soft Touch Keys. There Are 2 Lines On The LCD Display With Up To 31 Characters. The Spectro 2000 RS & Spectro 2000 RSP Have 10 Keys Each. There Are 2 Lines On The LCD Monitor. The Upper Line Shows The Current Wavelength A or T or C Value, And Cuvette Position Reference or S1, S2, S3. The Lower Line Shows The Menu And The Factor Of C Value, And Function Menu. The ESC and CHA and ACC Correspond To Escape, Change & Accept, and By Using Enter, Your Selection Is Approved. To Get The Menu, Use The Factor Key. Before Plugging The Spectro To The Power, Please Check To Be Sure The Switch At The Back Of The Spectro Is On 110V. 60Hz., Or 220V. 50Hz. (Whatever Your Lab Voltage Power Is). Please Convert The Power Switch As The First Step.

10 1. ABS 2. A/T/C A T C C = = = = = Adjusting 0 A And 100%T To Select One Of 3 Positions (A or T or C) Absorption Transmittance Concentration = (C=AxF) = (Log of T)

Has 2 Values MOL/L and MOL/ml

3. Factor or Function Can get A Menu And Roll The Menu, But Cannot Get Into The Menu Of The Lower Level. Structure Of 5 Menus Is Explained As Follows: (See Example For How To Set-Up) (1) PRINT SET-UP 1.1 EXT PRINT 1.2 INT PRINT 1.21 PAPER FEED 1.22 ALLOW PRINT EXT PRINT Refers To EXTERNAL PRINT; INT PRINT Refers To INTERNAL PRINT. (2) WAVELEG SET-UP (2.1 INITIAL WAVELG=550nm) (3) INT CONC 3.1 MOL/L 3.2 MOL/ML 3.3 PPB 3.4 PPM 4. LAMP ENERGY Presented By 8 Black Blocks

5. RS232 SET-UP

5.1 BAUD=9600 5.2 BAUD=4800 5.3 BAUD=2400 5.4 BAUD=1200

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4. PRINT When The Instrument Initializes Its Set-Up, It Defaults To External Print. If You Want To Change It, You Must Get Into The Menu To Change The Set-Up. Once Properly Set-Up, The Current State Can Be Printed Out. If It Is At A/T/C Or In Menu. THE NEXT TIME WHEN THE INSTRUMENT TURNS ON, IT WILL STILL HAVE THE PREVIOUS TIMES SET-UP. This Means That If The Last Time The Instrument Was Set At PRINT, This Will Become The New Default Until It Is Changed. Print Includes Internal Print And External Print. When It Is Set-Up To INT PRINT, You Can Set-Up The Paper Feed And Allow To Print Items (Refer To Example For How To Set-Up). If It Is Set-Up To Feed Paper, Touch PRINT And The Printer Will Go Two Lines Without Printing (Refer To Example). If It Is Set-Up To Allow Print, Touch PRINT, The Printer Will Print Out The Display And Current State (Refer To Example). If It Is In A Menu Of BAUD SET-UP, To Print Current Baud Set-Up Is ON, And Others Are OFF. If It Is At A/T/C, Touch PRINT, It Prints Current Wavelength, Cuvette Position And A/T/C Value. If It Is At C. It Prints Factor, Too. If You Want To Cancel The Current Set-Up Or Select Other Set-Up, You Must Return To The Menu To Make The Set-Up Again. 5 And 6. ESC And Enter ESC And ENTER Keys Correspond To Esc And (CHA, ACC) In The Menu. CHA Appeared At The Lower Right Corner Means Whether To Want To Change, ACC Means Whether To Want To Accept, ESC Means Whether To Want To Return. When The Instrument Is In Menu, It Only Responds To Multi-Functional Keys FACTOR, ESC, ENTER And PRINT Keys. If Any Other Key Is Touched, The Buzzer Will Send Out A Long Beeping Sound. When 2 Lines Are On The Display, The Cuvette Holder Will Not Move. A/T/C & Esc Should Be Touched To Clear The Second Line. Then The Cuvette Can Be Moved. When It Is At A/T/C, ESC And ENTER Keys Are CloseUp. If Touched, The Buzzer Will Send Out A Long Beeping Sound. When It Is At Master Menu, To Touch ESC Key, It Skips Out Of The Menu Set-Up And Return To A/T/C. If It Is At Sub-Menu, To ESC Key, It Returns To The Menu Of The Lower Level. When It Is At Master Menu, The Lower Right Corner Of The Display Screen Will Show CHA. If The Enter Key Is Touched, The Sub-Menu Set-Up Will Be Received. Only At The Lowest Level Of The Master Menu. FACTOR, will ACC Appear, Which Shows Whether To Want To Select This Item. If ENTER Is Touched, That Means This Item Is Accepted, And At The Same Time, It Returns To The Menu Of The Upper Level.

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When It Is At FACTOR Of C Value And Initial Wavelength Set-Up Of The Menu, The Lower Right Corner Of The Screen Will Appear CHA.

These Are Possible Options. If The ENTER Key Is Touched, It Can Change The Factor And The Initial Wavelength. When Changing the Wavelength. Push The Wavelength Button A Few Times.

When the Option Of making a change Is Reached, ENTER Must Be touched to ensure receipt of the data. If the ESC Key is touched, It will cancel the current set-up and return to the value before making the setup. 7 and 8 Multi-Functional Function 1:When it is at A/T/C, Wavelength can be changed. Range of wavelength is 325nm to 1100nm Function 2: When it is at Menu, It can roll the Menu. Function 3: When it is at FACTOR set-up of C and in the initial wavelength of the menu, the value wanted to set-up will automatically add 1, or subtract 1, yet it must be in ACC state, which means only when ACC is appeared at the lower right corner of the screen, can the value add 1 automatically. Otherwise, the function corresponds to Position 1. 9 & 10 MOVE SAMPLE When the Instrument is turned on, and after initialization, the cuvette is at the R position. R represents reference position S1, S2, S3 represents cuvette 1,2 and respectively. If touch , cuvette position will move R-S1-S2-S3-R at the same time, cuvette position will be displayed on the screen. If touch , cuvette position will move S3-S2-S1-R-S3 at the same time, cuvette position will be displayed on the screen. When Putting The Reference Cuvette In The First Cuvette Place. 0A And 100%T By A/T/C should be adjusted.

13 STORAGE MEMORIES: The Microprocessor System Will Store The Memory Of The Spectro 2000 RS & Spectro 2000 RSP, With The Same Set Up That Was In The Instrument Memory When The Spectro Was Last Turned Off. When The Instrument Is Turned On Again, All Of The Previous Set Up That Was Last Used Will Still Show On The Display. It Means That When The Instrument Was Last Turned Off, If It Was Set Up At INT PRINT, It Will Still Be Set Up At INT PRINT When The Instrument Is Put On The Next Time, Or If When The Instrument Was Turned Off, And The Wavelength Was Set Up At 600nm, The Spectro Will Show 600nm The Next Time The Instrument Is Turned On.

14 SUMMARY OF OPERATION 1. Convert The Voltage Power According To The Country Voltage Power (110V. or 220V.) 2. Turn The Power Switch On At The Back Of The Spectro 3. Display Shows: Spectro 2000 Please Wait

4. Sounds Of The Spectro Warming Up & Adjusting Will Now Be Heard. Wait Until The Spectro Is Completely Warmed Up,( THE SOUND WILL STOP) To Adjust The Cuvette Holder.

5. Display Shows:

6. If Different Wavelength Is Needed: Change The Wavelength By ( 7. If A or T Is Needed: Push The Soft Key A/T/C To See On The Display A or T or C.

8. Put The Cuvette With The Reference Solution In The First Cell Holder. The Sample For Testing In The Cell Holder Should Be Placed In Cell Holder 2, 3, or 4. The Display Will Show R (Reference) Or Either S2 - S3 - S4, By Pushing The Key S. Move (Sample Move). 9. The Spectro Will Show Setting Blank . When Putting The Reference In The First Cell Holder, Adjust A.000 And T100.00%. By Touching The Soft Key A/T/C A Few Times.

10. If The Second Line (Menu) Comes To The Screen Unwillingly, Push The A/T/C or ESC Key To Remove The Second Line. (Menu) Is The Second Line Of The Display That Exists In The Memory Of The Spectro. 11. Set Up The Factor For Reference Solution. 12. Put The Cell Holder 2, 3, or 4 And Test The Absorption, Transmission, Or Concentration. .

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PRINTING WITH THE SPECTRO 2000 RSP

Example A: If want to set-up internal print, do the following: 1. Touch F to get into the menu. 2. The first item of the menu is PRINT SET-UP,

3. Touch ENTER to get into print set-up. This item includes internal print and external print, that is EXT PRINT and INT PRINT,

4. Touch F or multi-function key ( up/down ) to get internal print. The internal print includes paper feed and allow print, that is PAPER FEED and ALLOW PRINT,

5. Touch F or multi-function key ( up/down ) to get allow print. 6. Touch ENTER key again, to accept this item. 7. Touch ESC a few times to clear the Display. 8. Touch PRINT to print information on the Display.

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Example B If want to set wavelength to 550nm-600nm when the instrument is turned on, do the following: 1. Touch FACTOR to get into the menu. 2. Touch FACTOR or multi-functional key ( up/down ) to get WAVELG SET-UP,

3. Touch ENTER again to set-up the initial wavelength

4. Touch ENTER key again, CHA at the lower right corner of the screen, changes to ACC which means to start accept.

5. Memory Storage Touch multi-function key ( up/down ) to get the initial wavelength to 600nm, then touch ENTER to accept 600nm. Next time when the instrument turns on, its wavelength will be at 600nm. Setting is completed and as it is shown in the figure. At that time, if ESC is touched, it will return to the inital set-up wavelength 550nm.

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Example C If want to set-up concentration to MOL/ML, do the following: 1.Touch FACTOR to get into the menu. 2. Touch FACTOR or multi-functional key ( up/down ) to get INIT CONC.

3. Touch again ENTER to get the first item of the menu of lower level, that is MOL/L

4. Touch FACTOR or multi-function key ( up/down ) to get the second item MOL/ML

Touch again ENTER to accept this item. At that time, at the upper line of the display, C is followed by a unit ml which represents MOL/ML

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Example D If want to test the lamp energy, do the following: 1. Touch FACTOR to get into the menu. 2. Touch FACTOR or multi-functional key to get LAMP ENERGY.

3. Touch again ENTER to get the lamp energy test. At that time, the lower line of the display will appear several black blocks to represent the lamp energy. There will be A Maximum Of 10 black blocks. In case ERR1 appears at the lower line, it means that the lamp energy is too strong. There is no need to change the lamp position. In case, ERROR appears at the lower line, it means that the lamp energy is too weak. It is necessary to adjust the lamp position, If The Lamp Energy Is Too Weak.

4. To adjust the lamp position use a screwdriver, and push the Lamp back and forth until most of the black blocks at the lower line reach the color Black completely (Until the Black Square Blocks are completely colored in). Which means that the position of the lamp has been adjusted to its optimal position.

19 Example E If want to set RS 232 Baud, do the following: 1. Touch FACTOR to get into the menu. 2. Touch FACTOR or multi-functional key ( up/down ) to get RS232 SET-UP

3. Touch ENTER to get the Baud selection. The first item is BAUD 9600.

4. Touch FACTOR or multi-function key ( up/down ) to reach the Baud wanted to set.

5. When turn to the BAUD that want to set, touch the ENTER key to select that BAUD Rate..

20 Example F If want to set-up the factor, do the following: 1. Touch A/T/C Key to get C. Factor (0.1-2000) 2. FACTOR = 1.0 will appear in the lower line of the screen

3. If ENTER key is touched ACC appears at the lower right corner of the screen, In order to change the factor.

4. Touch the multi-function key factor value will add 1 or subtract 1 automatically

5. If reach the value that want to set, touch the ENTER key to accept. The factor set-up is the value that was accepted. If do not want to accept, touch ESC to return to the previous value.