Catalogue Waters 2011 - 2012

720003820EN
2011 / 2012
2011 Waters Corporation. Printed in the U.S.A.
Waters Chromatography Columns and Supplies Catalog
www.waters.com
2011/2012
Waters Quality Parts, Chromatography

Columns and Supplies Catalog
SaleS OfficeS
Australia
Waters Australia Pty. Limited
Unit 3, 38-46 South Street
P.O Box 84
Rydalmere BC NSW 2116
Tel: 2 9933 1777
Fax: 2 9898 1455
Dear Colleague,
We are pleased to present the 2011 Waters Quality Parts,

Chromatography Columns and Supplies Catalog.
Our catalog contains todays leading separations technology products. With over 50 years of market
leadership we remain committed to the development and manufacture of products that solve todays most
challenging separations problems to enable our customers success. We are driven by our core mission to
provide enabling technologies in products that set the industry standard for performance and reproducibility.
Waters provides the industrys broadest and deepest product offering manufactured to industry
benc hmark quality requirements by our world-class manufacturing organization and supported by our
highly skilled sales and support teams.
We invite you to visit our new Online Order Center at www waters.com/order. T he online ordering
experience has been improved to make it easier and faster for you to obtain pricing and availability when
buying products.
T his year for the fist time our catalog is available in digital form. You may access and download it at
www.waters.com/catalog.
T his past year we launched several innovative, enabling products including our new CSH technology
that is the basis for the XSelect brand of HPLC columns and the ACQUITY CSH brand of UPLC columns,
the Ostro Sample Preparation plate for the removal of phospholipids in biological samples and the
Viridis line of columns for supercritical fluid chromatography.
To learn more about how we can help you achieve your objectives please telephone one of our 54 worldwide offices and interact with our highly trained account specialists.
Austria and European Export

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All Other Countries
Waters Corporation
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Milford, MA 01757 USA
Tel: 508 478 2000
800 252 4752
Fax: 508 872 1990
Michael J. Yelle
Senior Director, Chemistry Commercial Operations
Waters Corporation Milford, MA, U.S.A.
www.waters.com
CONTENTS
How to Order
Sample Preparation
10
Sample Vials
and Accessories
62
Filtration
80
ACQUITY UPLC Columns

and Consumables
86
Analytical Chromatography
106
Preparative Chromatography
177
Supercritical Fluid
Chromatography (SFC)
196
Bioseparations and Analyses
198
Size-Exclusion Chromatography
GPC & GFC
267
Supplies and Hardware
282
Alphabetical Index
328
Part Number Index
336
Cover Image:
Oasis HLB, a true binary mixed-mode, neutral copolymer, has a unique set of physicochemical properties that
delivers the benefits of simplicity, speed, and reproducibility in SPE methods development. Its combination
of water wettability and strong reversed-phase retentionenables high-throughput sample preparation
in 96-well plate formats and engenders confidence in analytical results.
Whats New
ACQUITY UPLC CSH Columns
XSelect HPLC Columns
Page 91
Page 118
CSH Technology is the next evolution of UPLC and HPLC particle
XSelect HPLC columns are the next evolutionary step in Waters
tec hnology. It affords outstanding batc h-to-batc h reproducibility,
LC column tec hnology. Based on Charged Surface Hybrid (CSH)
extended pH stability and seamless transferability between UPLC,
Tec hnology, this HPLC column family offers the widest range of
analytical HPLC and Preparative HPLC system platforms.
selectivity choices and superior peak shape improvements for basic
T he ACQUITY UPLC CSH column family provides separation scientists

wit h a wider range of selectivities as well as industry-leading
compounds when operated in acidic, low-ionic strength mobile

phases (e.g., 0.1% formic acid).
performance in simple, LC/MS-friendly mobile phases.
Ostro Sample Preparation Products
Waters Positive
Pressure-96 Processor
Page 53
Page 51
Ostro sample preparation products provide a novel solution for the
T he Waters Positive Pressure-96 Processor offers state of the art
cleanup of phospholipids in plasma and serum. Requiring minimal to
operation for 96-well and 1 cc flangeless cartridge formats. Each
no method development and using a simple protocol, this technology
of the 96 holes in the processor is restricted in order to maintain
can be quickly implemented in order to optimize your laboratorys
constant pressure, even if all the plate well positions are not filled.
workflow. Providing cleaner, more reproducible extracts t han

competitive phospholipid removal devices or tec hniques, the Ostro
96-well plate allows for more sensitive analyses, increased sample
throughput, and reduced instrument downtime.
www.waters.com
Whats New
Protein-Pak Hi Res
Ion-Exchange (IEX) Columns
ACQUITY UPLC BEH200

SEC Columns
Page 223
Page 217
The Protein-Pak Hi Res IEX column family consists of three ion-exchange
ACQUIT Y UPLC analytical size-exclusion c hromatography (SEC)
column chemistries each contained on monodisperse, non-porous particles
columns for the characterization of proteins and their aggregates. By
for the analysis of intact biomolecules including monoclonal antibodies,
applying the power of UPLC technology, you can now obtain accurate,
recombinant proteins, DNA/RNA and vaccine components.
precise, high-throughput quantitative analysis of proteins suc h as

therapeutic mAbs.
Viridis Supercritical Fluid

Chromatography (SFC) Columns
Online Order Center

and Digital Catalog
Page 196
Page 7
Combining state-of-the-art media manufacturing with the industry-
Waters Online Order Center streamlines the order process by placing
leading column technology, Viridis Supercritical Fluid Chromatography
product information, availability, tec hnical specifications and price
(SFC) columns bring a new level of reproducibility to the world of
in one central location.
laboratory-scale purification.
Combine the Online Order Center with Waters digital Chromatography

Columns & Supplies Catalog, and shopping with Waters has never
been easier.
www.waters.com
LITERaTURE ShOWCaSE
NEW ACQUITY UPLC CSH and

XSelect HPLC Columns Brochure
Lit. Co de 720003495EN
ACQUITY UPLC Columns Brochure

Lit. Code 720001140EN
ACQUITY UPLC
Column Strategies Brochure
XBridge HPLC Columns Brochure

SunFire HPLC Columns Brochure

Atlantis Columns Brochure

Prep OBD Columns Brochure

NEW Viridis SFC Columns Brochure

Oasis Sample Extraction

Products Brochure
Sample Vials and

Accessories Brochure
To view and download our

latest literature, simply visit
www.waters.com
and enter the literature code
in the search field (top right)
Sorbent Selection Guide

for SPE Wall Chart
Analytical Columns Wall Chart

Prep OBD Wall Chart

www.waters.com
LITERATURE SHOWCASE
NEW Ostro Sample

Preparation Products,
Waters DisQuE
Products for QuEChERS,
PoraPak RXN for

Post Synthesis Cleanup Brochure
Sample Preparation
Solutions Brochure
Bioseparations and
Analyses Catalog
Environmental
Analysis Catalog
Food Testing Solutions

Brochure
Environmental Analysis
Solutions Brochure
Oasis Sample Preparation

Application Notebook
Environmental Chromatography
Methods Guide
www.waters.com
Waters Vials Selector

www.waters.com/vialsselector
Waters Column eSelectivity Chart Version 3.0

www.waters.com/selectivitychart
Waters Preparative OBD Column Calculator

www.waters.com/prepcalculator
Waters Carbohydrate
Method Selection Tool
www.waters.com/amidemethod
NEW Waters Plate Selector

www.waters.com/plateselector
www.waters.com/filterselector
NEW Waters Filter Selector
LITERATURE SHOWCASE
Waters is committed to education and training. Learn from the best! Our expanding series of
easy-to-read, well-illustrated, high-quality primers, written by experts, introduce, inform,
and explain the latest technologies in analytical science.
The Quest for Ultra Performance in Liquid
Chromatography: Origins of UPLC Technology
From the dawn of LC to the present day, drawn almost entirely from original sources and first-person accounts, this
text reviews the first century of LC, showing how early the concepts of ultra performance were recognized and how
many decades it took to reduce them to practice. An extensive glossary is included.
Paperback, 54 pages,
ISBN 978-1-879732-05-6
Quest for Ultra Performance in Liquid Chromatography
715002098
Beginners Guide to Liquid Chromatography

Offering an uncomplicated introduction to the technology of liquid chromatography [LC], with a focus on HPLC, this
basic book uses clear language, colorful diagrams, and a full glossary to acquaint readers with basic concepts and
terminology. T his primer is suitable for younger science students as well as professionals new to LC.
ISBN 978-1-879732-02-5
715001531
Beginners Guide to UPLC (Ultra-Performance Liquid Chromatography)

Success is assured once new, experienced, and potential UPLC users learn from this volume the why and the how
of U PLC Tec hnology principles. Scientists will gain t he confidence to apply t his knowledge in ways t hat
en hance analytical productivity, streamline workflow, and advance scientific progress within their organizations.
ISBN 978-1-879732-07-0
Beginners Guide to UPLC
715002099
NEW Comprehensive Guide to HILICHydrophilic Interaction Chromatography

T his tec hnology primer is designed to provide the reader with the basic insight of how to be successful
with hydrophilic interaction c hromatography by understanding how the tec hnique works, the parameters
that impact retention and selectivity, as well as the practical considerations necessary to successfully
implement HILIC within a c hromatographic strategy.
ISBN 978-1-879732-08-7
NEW Comprehensive Guide to HILIC
715002531
The Mass Spectrometry Primer

A wide range of topics related to a broad spectrum of mass spectrometric techniques is covered in this volume. In it,
many frequently asked questions about the principles and practice of MS are answered. An extensive glossary explains
MS terminology, and the benefits of coupling MS with chromatography are amply described.
ISBN 978-1-879732-04-1
The Mass Spectrometry Primer
715001940
www.waters.com/primers
6
www.waters.com
How to order
Waters Online Order Center
An Improved Online Shoping Experience At Waters.com
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users to researc h, compare and purc hase consumables and spare parts online. We have now
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Ordering Waters Consumables and Spare Parts is quick and easy.
Quick Order
How to Buy Service Plans, Instruments,

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Order History
Exploded instrument diagrams help you

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Review product pricing
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conditions
Create a Wish List if you just

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Purchasing professionals regularly analyze their ordering and supply chains,
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If you are already using a Spend Management Solution or Internal
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Waters supports:
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PunchOut can be connected directly with internal purchasing software,
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2. B2B e-procurement integration with spend management systems
suc h as Ariba, SciQuest, SAP, Oracle, Perfect Commerce, Point
Systems, ReQlogic, Direct Commerce, OB10, etc.
3. Access to product information with catalogs hosted in your IT

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To request information on integration with procurement solutions
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Waters Shopping FAQs

W hether researc hing product information or making online purc hases, shopping
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Common Terminology & Definitions
Not Available online

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Local Representative for more information.
Orderable Part
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replacement part may be used in the same application.
[ Sample Preparation ]
The Importance of Sample Preparation ..................................................................................................................................................... 12
Sorbent Selection Guide ............................................................................................................................................................................ 13
Oasis Solid-Phase Extraction (SPE) Products
Introduction ................................................................................................................................................................................. 16
Oasis MCX for Basic Compounds ............................................................................................................................................... 18
Oasis MAX for Acidic Compounds ............................................................................................................................................. 19
Oasis WCX for Strong Basic Compounds................................................................................................................................... 21
Oasis WAX for Strong Acidic Compounds ................................................................................................................................ 22
Oasis HLB for Reversed-Phase SPE ............................................................................................................................................ 23
Oasis Elution Plates.................................................................................................................................................................. 26
Oasis 96-Well High-Throughput Extraction Plates ................................................................................................................... 28
Sensitivity and Precision ........................................................................................................................................................... 30
Sorbent Amount and Solvent Selection
for the Generic Method ............................................................................................................................................................... 31
Automation ................................................................................................................................................................................. 31
Oasis On-Line Columns .............................................................................................................................................................. 32
Oasis Glass Cartridges for PPT Detection Levels ...................................................................................................................... 33
Oasis Symbiosis On-Line Cartridges ......................................................................................................................................... 33
Oasis Product Selection Guide ................................................................................................................................................... 34
Oasis Sorbent Selection Tools ................................................................................................................................................... 35
Ordering Information ................................................................................................................................................................. 36
NEW Waters Positive Pressure -96 Processor ........................................................................................................................... 38
Sep-Pak Solid-Phase Extraction (SPE) Products
Introduction ................................................................................................................................................................................. 39
Sep-Pak Sorbent Selection Guide .............................................................................................................................................. 40
Sep-Pak Cartridge Selection Guide ............................................................................................................................................ 42
Sep-Pak 96-well Plates .............................................................................................................................................................. 42
Sep-Pak 96-well Elution Plate................................................................................................................................................. 43
Sep-Pak Specialty Chemistries ................................................................................................................................................... 44
Sep-Pak DNPH-Silica Cartridges......................................................................................................................................... 45
Ozone Scrubber Cartridges ................................................................................................................................................ 46
Waters XPoSure Cartridges ................................................................................................................................................ 46
PoraPak RDX Cartridges ...................................................................................................................................................... 47
Sep-Pak Dry SPE Cartridges................................................................................................................................................ 47
Certified Sep-Pak Cartridges ...................................................................................................................................................... 48
Certified Sep-Pak Sorbent Selection Guide ............................................................................................................................... 49
Waters Extraction Manifold ........................................................................................................................................................ 50
NEW Waters Positive Pressure -96 Processor ........................................................................................................................... 51
Environmental and Food Solution Kits ..................................................................................................................................................... 52
NEW Ostro Sample Preparation Products.................................................................................................................................................. 53
DisQuE Dispersive Sample Preparation Kit ............................................................................................................................................... 54
PoraPak RXN Cartridges for Post Synthesis Cleanup................................................................................................................................ 56
10
[ Sample Vials and Accessories ]

Waters Autosampler Vials ......................................................................................................................................................................... 62
Choosing the Right Vial and Septum for Your Application ..................................................................................................................... 62
Vial Closures Guide .................................................................................................................................................................................... 63
Vial Selection Guide................................................................................................................................................................................... 63
Screw Cap 12 x 32 mm Vials for ACQUITY UPLC Systems .................................................................................................................... 64
Waters ACQUITY UPLC System Sample Manager .................................................................................................................................... 65
Plates for ACQUITY UPLC Systems ........................................................................................................................................................... 66
Glass Inserts for 96-Well Plates ............................................................................................................................................................... 66
Heat Seals................................................................................................................................................................................................... 67
Most Commonly Used Vials for Alliance Systems .................................................................................................................................. 68
Plates for Alliance HT Systems................................................................................................................................................................. 69
Screw Cap 12 x 32 mm Vials for Alliance Systems ............................................................................................................................... 70
Snap Cap 12 x 32 mm Vials for Alliance Systems................................................................................................................................. 71
Vials for Waters 717 Autosampler........................................................................................................................................................... 72
Vials for Waters 2707 Autosampler ........................................................................................................................................................ 73
Screw Cap 12 x 32 mm Vials for Compatible Systems .......................................................................................................................... 74
Snap and Crimp Cap 12 x 32 mm (9 mm Cap) Vials for Compatible Systems..................................................................................... 75
15 x 45 mm Vials for Compatible Systems ............................................................................................................................................ 76
GPC 2000 Vials.......................................................................................................................................................................................... 76
Aqua Analyis System Vials ....................................................................................................................................................................... 76
Settings for Alliance 2690 and 2695 Vials and Low Volume Inserts (LVI) ......................................................................................... 77
Vial Descriptions ........................................................................................................................................................................................ 78
Vials Troubleshooting Guide ...................................................................................................................................................................... 79
[ Filtration ]
Sirocco Protein Precipitation Plate............................................................................................................................................................ 80
Pall Life Sciences Sample and Solvent Filtration Products ..................................................................................................................... 81
Filter Design and Membrane Choices........................................................................................................................................................ 82
Part Number Cross Reference .................................................................................................................................................................... 83
Solvent Filtration Membranes ................................................................................................................................................................... 83
Solvent Filtration Apparatus ..................................................................................................................................................................... 83
Syringe Filters Product List ....................................................................................................................................................................... 84
11
SaMPLE PREPaRaTION
T he Importance of Sample Preparation
In the last two decades, dramatic advances in analytical instrumentation

and laboratory information management systems shifted the analysts
predominant tasks from assay measurements to sample preparation and
data processing. As the stringency of requirements for higher sensitivity,
selectivity, accuracy, precision, and number of samples to be processed has
escalated, the corresponding increases in speed and sophistication of analysis and data collection have outpaced improvements in the many traditional
techniques of sample collection and preparation. By some estimates, 75 to
80% of the work activity and operating cost in a contemporary analytical
lab is spent processing and preparing samples for introduction or injection
into an analytical separation and/or measurement device. Clearly, efforts
directed and products designed to streamline sample preparation protocols
are essential to future progress in analytical science.
Goals of Sample Preparation

Successful sample preparation for most analytical techniques [HPLC, GC,
spectrophotometry, RIA, etc.] has a threefold objective: namely, to provide
the sample component of interest
Benefits of Solid-Phase Extraction

(SPE) Cartridges
W hen compared to other sample preparation processes, solid-phase
extraction using SPE cartridges offers:
lower cost
no emulsion problems
lower solvent consumption
less sample handling
greater recoveries
fewer steps
lower reagent consumption
minimal sample transfer
no transporting of
samples to lab
less apparatus
direct field sampling
faster protocol
fewer steps
reduced harm to
labile samples
greater safety
minimal evaporation
minimal glass breakage
less glassware used,

less to wash
in solution
less exposure to toxic agents
free from interfering matrix elements
greater accuracy
at a concentration appropriate for detection or measurement.
no cross contamination
12
Sample Preparation
www.waters.com
SaMPLE PREPaRaTION
SPE Application Area/Sorbent Selection Guide

Matrix Key
Air
Sorbent/Surface
Petroleum
Common
Sample Matrices
Biological
Description
Soil
Water
Food
Typical Applications
Properties
Reversed-Phase
Oasis HLB
N-VinylpyrrolidoneDVB copolymer
Sep-Pak tC18
Silica
SiC18H37
Sep-Pak C18
Silica
Si(CH3)2C18H37
Sep-Pak C8
Silica
Si(CH3)2C8H17
Sep-Pak tC2
Silica
SiC2H5
Strongly hydrophobic, water-wettable polymer with

unique hydrophilic-lipophilic balance. Maintains high
retention and capacity even if it runs dry after
conditioning. Stable in organic solvents.
Drugs and metabolites in biofluids

Isolation of peptides and oligonucleotides
High-throughput biopolymer desalting
Trace organics, priority pollutants, endocrine disrupters
JPMHLW official food methods: antibiotics, pesticides
Particle sizes: 30 and 60 m

Pore size: 80
Surface area: 830 m2/g
pH range: 014
Strongly hydrophobic, silica-based bonded phase used to

adsorb analytes from aqueous solutions. Trifunctional
bonding chemistry for increased hydrolytic stability.
JPMHLW official methods for pesticides in water

JPMHLW official methods for odorants in water
Particle size: 3755 m

Pore size: 125
Carbon load: 17%
pH range: 28
Hydrophobic, silica-based bonded phase used to adsorb

analytes from aqueous solutions. Monofunctional
bonding provides alternate selectivity versus tC18.
Lipid fractionation; ganglioside isolation

Organic acids in fruit juice, wine
JPMHLW and CDFA official methods: pesticides in food
Natural products
AOAC methods for food colors, sugars

Pore size: 125
Carbon load: 12%
pH range: 28
Moderately hydrophobic, silica-based bonded phase used

in methods when less retention than that of HLB or C18
is required.
Drugs and their metabolites in biofluids

Peptides in serum and plasma

Pore size: 125
Carbon load: 9%
pH range: 28
Weakly hydrophobic, silica-based bonded phase used

in methods when less retention than that of C8 is
required. Trifunctional bonding chemistry for increased
hydrolytic stability.
Applications are similar to those of C18 and C8

Pore size: 125
Carbon load: 2.7%
pH range: 28
Moderately polar, silica-based bonded phase with

weakly basic surface. Can be used as a polar sorbent
with different selectivity for acidic/basic analytes
or as a weak anion exc hanges in aqueous medium
below pH 8.
Phenols, phenolic pigments, natural products

Petroleum fractionation
Saccharides
Drugs and drug metabolites
JPMHLW official methods for pesticides in food

Pore size: 125
Carbon load: 3.5%
pH range: 28
Silica-based bonded phase with low hydrophobicity can

be used as a less polar alternative to silica or as a less
hydrophobic alternative to C18 or C8.
Drugs and their metabolites

Pesticides

Pore size: 125
Carbon load: 6.5%
pH range: 28
Moderately polar, neutral, silica-based bonded phase.

Used in normal-phase applications where acidic
c haracter of silica is undesirable or as a weakly
hydrophobic phase in aqueous media.
Antibiotics in cosmetics
Protein and peptide isolation by HIC
[ hydrophobic-interaction chromatography]

Pore size: 300
Carbon load: 2%
pH range: 28
Reversed- or Normal-Phase
Sep-Pak
Aminopropyl
Silica
Si(CH2)3NH2
Sep-Pak
Cyanopropyl
Silica
Si(CH3)(CH2)3CN
Sep-Pak Diol
Silica
Si(CH2)3OCH2CH(OH)
CH2OH
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Sample Preparation
13
SaMPLE PREPaRaTION
Sorbent/Surface
Common
Sample Matrices
Description
Properties
Normal-Phase
Sep-Pak Silica
SiO2
Sep-Pak Florisil
MgOSiO2
Sep-Pak Alumina
(A,N,B)
Al2O3
Polar sorbent binds analytes in non-aqueous solvents.

Also used as an intermediate-strength cation exchanges
in aqueous media and as a support for liquid-liquid
partition separations.
Vitamins and food additives

Lipid classification
Synthetic organic compounds
Natural products, plant pigments

Pore size: 125
Activity: High [ 3.2% water]
Polar, highly active, weakly basic sorbent for the

adsorption of low to moderately polar species from
non-aqueous solutions.
AOAC and EPA official methods for pesticides

Polychlorinated biphenyls [ PCBs] in transformer oil

Pore size: 60
Activity: High [ 2.5% water]
pH of 10% aqueous slurry: 8.5
Highly surface-active polar, acidic [A], neutral [N], and

basic [B] sorbents. Exhibits specific pi-electron interactions
with aromatic hydrocarbons. Acidic and basic alumina
are also low-capacity ion exchangers in aqueous media,
unaffected by high-energy radioactivity.
Petroleum, synthetic crude oil fractionation [N]

Radioactive compound isolation, isotope generators [A,B]
Phospholipids, steroids, catecholamines [B]
Food, feed additives [A,N], synthetic organic compounds [N]
Pesticide, herbicide, priority pollutant isolation [N,B]
Alternative to official AOAC and EPA methods [A,N,B]

Pore size: 120
Activity: High, 1 on Brockmann
scale [ 1.5% water]
pH of 10% aqueous slurry:
A: 4 N: 7.5 B: 10
Prepackaged centrifuge tube containing anhydrous

sodium acetate/anhydrous magnesium sulphate and
anhydrous magnesium sulphate/primaryl secondary
amine sorbent. Used for dispersive solid-phase extraction, commonly referred to as QuEChERS.
Food Safety: Monitor commodities for chemical residues

to ensure compliance with established regulation
Food Quality and Manufacture: Quality control of
manufactured products
AOAC Method 2007.01
Properties: DisQuE Extraction [Tube 1]:

A 50 mL centrifuge tube containing 1.5 g
anhydrous sodium acetate and 6 g of
anhydrous magnesium sulphate.
DisQuE Clean-Up [Tube 2]: A 2 mL
centrifuge tube containing150 mg anhydrous
magnesium sulphate and 50 mg of primary
secondary amine [PSA] sorbent.
Mixed-mode, reversed-phase/strong cation-exchange,

water-wettable polymer. Highly selective for bases.
Stable in organic solvents.
Basic drugs from biofluids and tissue extracts

Drug monitoring: screening, identification, confirmation,
and quantitation
Pesticides, herbicides

Pore size: 80
pH range: 014 [pKa: < 1]
IEX capacity: 1 meq/g
Mixed-mode, reversed-phase/weak cation-exchange,

water-wettable polymer. Used to retain and release
strong bases. Stable in organic solvents.
Strongly basic compounds in biofluids and tissue extracts

and quantitation
JPMHLW official method for streptomycin and
dihydrostreptomycin in vegetable crops

Pore size: 80
pH range: 014 [pKa: ~ 5]
IEX capacity: 0.75 meq/g
Silica-based, hydrophilic, weak cation exchanger with

large pore size used to extract cationic analytes in
aqueous and non-aqueous solutions.
Isolation of cationic proteins

Pesticides, herbicides
Steroids
Inorganic cations in environmental samples
Particle sizes: 3755 m

Pore size: 300
pH range: 29
Ligand density: 350 moles/g
Protein-binding capacity:
175 mg Cytochrome c/g
Mixed-mode, reversed-phase/strong anion-exchange,

water-wettable polymer. Selective for acids. Stable in
organic solvents.
Acidic compounds and metabolites in biofluids and

tissue extracts
and quantitation
Food additives and contaminants

Pore size: 80
pH range: 014 [pKa: > 18]
Silica-based, hydrophilic, strong anion exchanger with

large pore size used to extract anionic analytes in
aqueous and non-aqueous solutions.
Isolation of anionic proteins

Acidic pigments in wine, fruit juices, food extracts
Phenolic compounds
Peptide pool fractionation
Inorganic anions in environmental samples
Particle sizes: 3755 m

Pore size: 300
pH range: 29
Ligand density: 220 moles/g
Protein-binding capacity:
200 mg BSA/g
Mixed-mode, reversed-phase/weak anion-exchange, water

wettable polymer. Used to retain and release strong
acids. Stable in organic solvents.
Strongly acidic compounds and metabolites in biofluids

and tissue extracts
and quantitation
Emerging contaminants

Pore size: 80
pH range: 014 [pKa: ~ 6]
Silica-based bonded phase containing primary and

secondary amines. Similar selectivity to aminopropyl but
with higher pKas and increased ion-exchange capacity.
Strong affinity for fatty acids, polar pigments, and sugars

Potential for adsorption by chelation
Particle sizes: 37-55 m

Pore size: 60
pH range: 2-9
IEX capacity: 1.75 meg/g
Dispersive
DisQuE
Specific Application
Ion-Exchange and Mixed-Mode

Oasis MCX
N-Vinylpyrrolidone
DVB copolymer
SO3H
Oasis WCX
N-Vinylpyrrolidone
DVB copolymer
COOH
Sep-Pak Accell
Plus CM
Acrylic acid/acrylamide
copolymer on diol-silica
COO Na+
Oasis MAX
N-Vinylpyrrolidone
DVB copolymer
CH2N(CH3)2C4H9+
Sep-Pak Accell
Plus QMA
Acrylamideacrylic acid
copolymer bound
to diol-silicaC(O)
NH(CH2)3N(CH3)3+ Cl-
Oasis WAX
N-VinylpyrrolidoneDVB
copolymerCH2piperazine
Sep-Pak PSA
SiC2H4NHC2H4NH2
14
Sample Preparation
www.waters.com
SaMPLE PREPaRaTION
Sorbent/Surface
Common
Sample Matrices
Description
Properties
Products for Environmental and Food Analysis

Sep-Pak DNPH
Acidified dinitrophenylhydrazine reagent coated on silica

used for collection of air samples. Aldehydes and ketones
react in situ to form hydrazone derivatives; these are then
eluted and quantitated by HPLC analysis.
EPA Method TO11A; ASTM D5197 for carbonyl

compounds in air
JPMOE Official Methods for aldehydes: odor in out
door air and in exhaust gas

Pore size: 125
Recommended maximum capacity:
75 g [2.5 moles]
formaldehyde/cartridge
Acidified dinitrophenylhydrazine reagent coated on silica used

for collection of air samples. Aldehydes and ketones react in
situ to form hydrazone derivatives; these are then eluted and
quantitated by HPLC analysis. Larger particle size optimized
for low-pressure personal air monitors.
JPMHLW official methods for aldehydes in indoor air

EPA Methods TO11A and IP6A, ASTM D5197 for
carbonyl compounds in air
NIOSH Method 2532 for glutaraldehyde in air

Pore size: 125
Recommended maximum capacity:
70 g [2.3 moles]
formaldehyde/cartridge
Potassium iodide cartridge is used in series with Sep-Pak

DNPH and X PoSure Aldehyde Sampler cartridges to
remove ozone interferences.
EPA Method IP6A and ASTM D5197 for carbonyl

compounds in air
Quantity: 1.4 g KI
Capacity: 4.2 mmoles
ozone/cartridge [theoretical]
High-capacity desiccant used to remove residual water

from normal-phase SPE extracts [in water-immiscible
organic solvents].
General purpose
Quantity: 2.85 g
anhydrous Na2SO4
T heoretical capacity: 3.6 g H2O
Hydrophobic polymer for explosives analysis.
Explosives in ground and surface water at ppb level

EPA Method 8330 for explosives

Pore size: 200
Typical sample volume:
500 mL water/cartridge
Very hydrophobic copolymer designed for multi-residue

pesticide analysis in water samples.
JPMHLW official methods for pesticides in water

Particle size: 80 m
Quantity: 265 mg/cartridge
Activated carbon
Highly hydrophobic, low ash content, activated carbon

used to remove or enrich very polar organic molecules
from water.
JPMHLW official method for 1,4-dioxane

analysis in water
Pesticides, herbicides, esp. highly polar small molecules
Particle size: 85 m
Quantity: 400 mg/cartridge
Sep-Pak Carbon
Black/Aminopropyl
Two-layer sorbent bed used for pesticide cleanup in food

matrices prior to GC analysis.

JPMHLW official method for propham

[carbon black, top layer]
55105 m [aminopropyl silica]
Quantity: 500 mg of each sorbent,
separated by frit
Two-layer sorbent bed used for pesticide clean-up in food

matrices prior to GC analysis. PSA provides alternative
selectivity compared to aminopropyl.
JPMHLW official methods for pesticides in food.
Particle size: 37-105 m

[carbon-black, top layer]
37-55 m [ PSA, bottom layer]
Quantity: 500 mg of each sorbent,
separated by frit
Diphenylhydrazine coated
on silica
Sep-Pak X PoSure
Aldehyde Sampler
Diphenylhydrazine coated
on silica
Sep-Pak Ozone
Scrubber
Potassium iodide
Sep-Pak Dry
Anhydrous Sodium Sulfate
Sep-Pak
Porapak RDX
DVBN-Vinylpyrrolidone
copolymer
Sep-Pak PS2
StyreneDVB copolymer
Sep-Pak AC2
Carbon black
Aminopropyl silica
Sep-Pak Carbon
Black/PSA
Primary-Secondary
Amine Silica
*AOAC = Association of Official Analytical Chemists; ASTM = American Society for Testing and Materials [International]; CDFA = California Department of Agriculture; EPA = U.S. Environmental Protection Agency;
JPMHLW = Japanese Ministry of Health, Labour and Welfare; JPMOE = Japanese Ministry of the Environment; NIOSH = National Institute for Occupational Safety and Health.
Matrix Key
Air
www.waters.com
Petroleum
Biological
Soil
Water
Sample Preparation
Food
15
SaMPLE PREPaRaTION
A Breakthrough in SPE
Introduction
In October 1977, Waters designed the first miniature cartridge columns
(Sep-Pak cartridges) containing silica-based adsorbents for SPE.
Demands for sample preparation led to the development of a speciallydesigned polymeric sorbent that performs optimally for reversed-phase
SPE. T he Oasis HLB copolymer with unique Hydrophilic-Lipophilic Balance
is unlike traditional SPE sorbents.
Todays goals for modern solid-phase extraction (SPE) are faster throughput,
higher recovery and reproducibility, and stronger retention and selectivity.
Now SPE can outpace high-throughput techniques such as LC/MS/MS.
Unique Water-Wettable Oasis HLB Copolymer
T he Oasis HLB sorbent is a macroporous copolymer made from a balanced

ratio of two monomers, the lipophilic divinylbenzene and the hydrophilic
N-vinylpyrrolidone. It provides reversed-phase capability with a special
polar hook for enhanced capture of polar analytes and excellent wettability.
High and Consistent Recoveries

Oasis sorbents are water-wettable maintaining high retention and capacity
for a wide spectrum of analytes, especially when the SPE column runs dry.
W hen the sorbent pores dry out, the c hromatographic retention (capture)
of the analytes is reduced, resulting in poor recovery. Traditional, silicabased C18 sorbents can easily dry out, especially on a vacuum manifold
if a particular cartridge flows quickly and allows air to be drawn in. Oasis
sorbents maintain proper wetting for more consistent performance
(especially important for 96-well plates). Even if air passes through, the
Oasis pores do not dry out.
Pore Dewetting Mechanism of Sorbent Pores (Silica Based C18)
N-VINYLPYRROLIDONE
Analytes
properly
retained
in pore
DIVINYLBENZENE
Properly Wetted Pore

HYDROPHILIC-LIPOPHILIC BALANCE
Specific Surface Area: 810 m2/g, Average Pore Diameter: 80
Total Pore Volume: 1.3 cm3/g, Average Particle Diameter: 30 mm or 60 mm
Analytes
not
retained
Dewetted Pore
If air passes through traditional
C18 cartridge the pores dry out.
When sample is loaded, the analytes are
not retained resulting in poor recoveries.
Current Oasis Patents:

Patent No. 5,882,521 (1996), Patent No. 5,976,376 (1998), Patent No. 6,106,721 (1999), Patent No. 6,254,780 (2001)
Patent No. 6,322,695 (2001), Patent No. 6,468,422 (2002), Patent No. 6,726,842 (2004), Patent No. 6,773,583 (2004)
Patent No. 6,723,236 (2004), Additional Patents Pending
16
www.waters.com
SaMPLE PREPaRaTION
Exceptional Batch-to-Batch Reproducibility
Effect of Drying on RecoveryOasis HLB Versus C18 Sorbents
Because of poor stability at pH extremes and relatively low ionic capacity

traditional silica-based, mixed-mode sorbents dont have long-term batchto-batch reproducibility and therefore require reservations of specific lots of
sorbent for large projects. Oasis sorbents have demonstrated excellent longterm, batch-to-batch reproducibility for over ten years. As a result of careful
process design and stringent quality controls, a new standard has been set
in batch-to-batch and lot-to-lot reproducibility for SPE sorbents. T he Oasis
family of sorbents and devices are manufactured in a Waters ISO 9002
registered facility in compliance with cGMP guidelines of the U.S. Food and
Drug Administration for class 1 medical devices.
No impact of sorbent drying on HLB

High, consistent recovery
C18 Cartridge (100 mg)
100
100
80
80
Procainamide
Acetaminophen
Ranitidine
Propranolol
Doxepin
60
40
20
0
5
Drying Time
% Recovery
% Recovery
Oasis HLB Cartridge (30 mg)
60
40
20
10 Min.
4
Drying Time
8 Min.
Oasis HLB 1 cc/30 mg and 1 cc/100 mg C18 cartridges were conditioned on a Waters vacuum
manifold. When the methanol reached the top of the upper frit in each cartridge, vacuum was
maintained for different times to vary the cartridge drying time. The SPE protocol was then continued.
The data shown are the average of three replicate extractions.
Multiple batches of each Oasis HLB, MCX, and MAX have been successfully
used on validated bioanalytical assays in a regulated laboratory environment.
Batch-to-Batch Reproducibility of Oasis HLB Sorbent

No batch reservations needed: consistently high recoveries over more than 10 years of production
T he variable recoveries seen with the C18 sorbents, due to the drying out
effect, are often the cause for retests, reducing laboratory productivity. In
some laboratories 10% of samples are reteststhis can be reduced using
Oasis sorbents.
Also, Oasis sorbents retain polar compounds far better than bonded silica
SPE sorbents. Note the poor recovery of the polar analyte Acetaminophen
for C18. Oasis sorbents work especially well when you need to capture
metabolites (see figure above).
High CapacityUse Less Sorbent

When transferring methods from a C18-bonded phase to Oasis products, keep
in mind the greater capacity of the Oasis sorbent. The Oasis sorbent has
2-3X more surface area and shows a dramatic increase in k values compared
to silica-based C18, This reduces breakthrough potential. In addition,
you may be able to use 2/3 less sorbent than you would with C18 (30 mg
Oasis HLB gives equivalent capacity to 100 mg C18).
% Recovery
105
95
85
Recovery for:
Procanamide 1.90% RSD

Ranitidine 1.68% RSD
Acetaminophen 1.65% RSD
Oasis SPE Applications

Oasis products come in a full range of device formats to meet your SPE
requirementsElution plates, on-line columns, 96-well plates, and
single-use cartridges.
Try Oasis and successfully meet your SPE c hallenges.
Download your free Oasis Applications Notebook
at www.waters.com/oasis
Higher Retention Means Greater Capacity, No Breakthrough

Retention Factor (k) Comparison
C18 Cartridge
H O
Oasis HLB Cartridge
Literature
References
O
HO
CH
N
O
H
O
N
N
CH
3X
Oasis Sample Extraction Products Brochure,

Literature Reference 720001692EN
Theobromine
2X
Oasis Elution Plate Brochure,

N
3
Acetaminophen
H
N
H3C
Salicylic acid
3
6X
OH
HO
O
HO
Catechol
15X
0
50
100
Data shown were obtained with two 3.9 x 150 mm columns, each packed with one of the sorbents, operated under
the same conditions: mobile phase: 20 mM potassium phosphate, pH 7.0/methanol (95/5 v/v); temperature: 30 C;
flow rate: 1.0 mL/min; detection: UV @ 254 n
www.waters.com
Topics in Solid-Phase Extraction.

Part 1. Ion Suppression in
LC/MS Analysis W hite Paper,
Sample Prep Solutions Brochure,
Oasis WAX Sorbent for UPLC/MS

Determination of PFOS and Related
Compounds in Waters and Tissue,
SPE Sample Preparation for
UPLCMS Determination of
Enrofloxacin (Baytril) in Chicken,
Literature Reference WA43206
A Sensitive Method for the Determination
of Endocrine-Disrupting Compounds in
River Water by LC/MS/MS,
17
SaMPLE PREPaRaTION
Oasis MCX for Basic Compounds
Obtain selective retention of basic drugs with cation-exc hange groups

on the sorbent surface. T he Oasis MCX sorbent has a tightly controlled
ion-exchange capacity (1 meq/g). T here are no silanol groups to complicate
the retention mode or method development. T his novel, water-wettable,
polymeric sorbent is stable from pH 0 to 14, making method development
simple and fast.
Drug/Sorbent Interactions on Oasis MCX Sorbent
Oasis MCX Retention Map for Basic Analytes

Impact on Retention Factor (k) for a Base by Changing Mobile
Phase pH in Cation-Exchange and Reversed-Phase Mode
Base (Ionized)
60
50
Retention (k)
Oasis MCX Mixed-Mode Cation eXchange and Reversed-Phase Sorbent

for High Selectivity and Sensitivity for Basic Compounds
Base (Unionized)
40
Cation Exchange
30
20
Reversed Phase
10
Highly selective retention enables much stronger washes,

resulting in very clean extracts.
9 10 11 12 13 14
Mobile Phase pH
O
Reversed-Phase
Retention
MCX sorbent
Mixed-mode Cation eXchange
Basic Analyte
(Combined)
Retention
SO3
SO3
Strong cationexchange mode

Best retention at least
2 pH units below pKa
of the analyte
Reversed-phase
interaction
CH 3
N
H H
O
Sulfonic-acid-cationexchange capacity:
1.0 meq/g
Cation-Exchange
Retention
CH 3
OH
Propranolol
(basic drug)
Since this ion-exc hange sorbent is synthesized from the reversed-phase

Oasis HLB copolymer, it features two retention mec hanisms (cationexchange and reversed-phase) which can be manipulated very predictably
(please refer to the Oasis MCX Retention Map).
T his Retention Map plots the total k or capacity (retention) of a basic

analyte relative to pH. Note that the total k, is the sum of the two retention
mechanisms. At low pH, the analyte is charged and experiences maximum
retention primarily from the ion-exchange mechanism, however, there is
also a slight amount of reversed-phase contribution for the combined retention. If your goal is to capture basic analytes and then wash out interferences aggressively, the Load and Wash steps should be at low pH to obtain
maximum capture.
At high pH, the ion-exchange retention mechanism shuts-off because the
analyte becomes un-ionized. Only reversed-phase retention is present, but
since the analyte is now un-ionized, we get the maximum of the reversedphase retention. We can elute with a combination of high pH and high
organic concentration.
Generic Oasis MCX Method
for Extraction of Basic Compounds
Condition:
CH3OH
Equilibrate:
H2O
Load:
Spiked and acidified sample
Wash 1:
2% HCOOH in H2O
Elute 1(Wash 2):
CH3OH
Elute 2:
5% NH4OH in CH3OH
Evaporate and Reconstitute:
20% CH3OH in H2O
18
www.waters.com
SaMPLE PREPaRaTION
Oasis MAX for Acidic Compounds
Oasis MAX Mixed-Mode Anion eXchange and Reversed-Phase Sorbent
for High Selectivity and Sensitivity for Acidic Compounds
T he Oasis MAX sorbent has a tightly controlled ion-exchange capacity of
0.3 meq/g ensuring reproducible SPE protocols for extraction of acidic
compounds and metabolites from biological fluids. T here are no silanol
groups to complicate the retention mode or method development. T his
novel, water-wettable, polymeric sorbent is stable from pH 0 to 14, making
method development simple and fast.
Drug/Sorbent Interactions on Oasis MAX Sorbent

MAX sorbent
Mixed-mode Anion eXchange
CH 3
+
CH 2 N
Since this ion-exchange sorbent is synthesized from the reversed-phase

Oasis HLB copolymer, it features two retention mec hanisms (anionexchange and reversed-phase) which can be manipulated very predictably
(please refer to the Oasis MAX Retention Map, below).
R+
Reversed-phase
retention
Quaternary-amine-
Oasis MAX Retention Map for Acidic Analytes

Impact on Retention Factor (k) for an Acid by Changing
Mobile Phase pH in Anion-Exchange and Reversed-Phase
Acid (Ionized)
60
Retention (k)
Acid (Unionized)
30
Anion Exchange
20
Reversed Phase
10
0
9 10 11 12 13 14
Anion-Exchange
Retention
Acidic Analyte
(Combined)
Retention
T he Retention Map plots the total k or capacity (retention) of an acidic

analyte relative to pH. Note that the total k, is the sum of the two retention
mechanisms. At high pH, the analyte is charged and experiences maximum
retention primarily from the ion-exchange mechanism, however, there is
also a slight amount of reversed-phase contribution for the combined
retention. If your goal is to capture acidic analytes and then wash out
interferences aggressively, the Load and Wash steps should be at high
pH to obtain maximum capture.
www.waters.com
CH 3
Suprofen
(acidic drug)
At low pH, the ion-exchange retention mechanism shuts-off because the

analyte becomes un-ionized. Only reversed-phase retention is present, but
since the analyte is now un-ionized, we get the maximum of the reversedphase retention. We can elute with a combination of low pH and high
organic concentration.
Condition:
CH3OH
Equilibrate:
H2O
Load:
Spiked acidified sample
Mobile Phase pH
Reversed-Phase
Retention
Generic Oasis MAX Method

for Extraction of Acidic Compounds
50
40
Strong anionexchange mode

Best retention at least
2 pH units above pKa
of the analyte
COO
anion-exchange capacity:
0.25 meq/g
C4H9
CH 3
Wash 1:
5% NH4OH in H2O
Elute 1 (Wash 2):
CH3OH
Elute 2:
2% HCOOH in CH3OH
20% CH3OH in H2O
As shown (next page), acidic compounds can be separated from basic

and neutral compounds on the same Oasis MAX cartridge following the
recommended protocol.
19
SaMPLE PREPaRaTION
High Recovery of Acidic, Basic, and Neutral Compounds Using the Generic Oasis MAX Method
Elute 1 (basic and neutral drugs)
Elute 2 (acidic drugs)
2
I.S.
AV
AV
0
Column:
Mobile Phase:
Detector:
Temperature:
Flow Rate:
Injection Volume:
SymmetryShield 3.5 m, 4.6 x 100 mm

SymmetryShield Guard column, 5 m,
3.9 x 20 mm
20 mM potassium phosphate,
pH 2.7/methanol, 52:48 v/v
UV @ 214 nm
30 C
2 mL/min
10 L of extract with internal standard
I.S.
Sample
Sample
Blank with I.S.
Blank with I.S.
10 Min.
10 Min.
Results (n=6)
Peak Identification
1. Nortriptyline
2. Secobarbital
3. Ketoprofen
4. Naproxen
I.S. Butylparaben
Drugs
Nortriptyline
Secobarbital
Ketoprofen
Naproxen
g/mL
5
10
5
2.5
Type of Drug
Basic (Elute 1)
Neutral (Elute 1)
Acidic (Elute 2)
Acidic (Elute 2)
% Recovery
% RSD
94.34
88.53
91.64
103.43
0.78
1.03
2.35
3.04
* 1 mL urine hydrolyzed (1M KOH 60 C, 15 minutes) and adjusted to pH 2 with

phosphoric acid; diluted 1:1 with 10 mM sodium acetate, pH 2.
Literature
References

PST T herapeutic Peptide Method Development Kit

T he PST T herapeutic Peptide Method Development Kit has been developed to
simplify the process of sample preparation and LC method development for
the analysis of therapeutic peptides in plasma. T he kit contains an Oasis PST
Elution Method Development Plate, a PST 300 C18 reversed-phase column
and the detailed screening protocol.
See Page 209
20
www.waters.com
SaMPLE PREPaRaTION
Oasis WCX for Strong Basic Compounds
High Selectivity and Sensitivity for Strong Basic Compounds
Generic Oasis WCX Method for

Strong Basic Compounds

Condition:
CH3OH
Equilibrate:
H2O
WCX sorbent
Weak Cation eXchange
Load:
Spiked acidified sample
COO -
Wash 1:
5% NH4OH in H2O
Weak cation-exchange
interaction
CH3
H 3C
O
O
Reversed-phase
interaction
Elute 1 (Wash 2):

CH3OH
CH3
N
CH3
Elute 2:
2% HCOOH in CH3OH
CH3

20% CH3OH in H2O
Carboxylic-acid-cationexchange capacity: 0.75 meq/g
T he Oasis WCX (Weak Cation eXchange) SPE material was developed to

provide better sample preparation for strong bases and quaternary amines.
T he retention mechanism is mixed mode, both ion exchange and reversed
phase, which improves retention for all types of basic analytes, especially
strong bases.
Recovery Data for T hree Basic Compounds from
Oasis WCX vs. Silica-Based WCX Products
(a)
Excellent Recovery for Quaternary Amines as

well as Polar and Hydrophobic Bases
Column:
Mobile Phase A:
Mobile Phase B:
Gradient:
Flow Rate:
Injection Volume:
Oasis WCX 96-well Elution Plate

120.0
Recovery (%)
100.0
80.0
Protriptyline (H)
60.0
Valethamate (Q)
40.0
Atenolol (P)
XTerra MS C18 IS 2.1 x 20 mm, 3.5 m

10 mM NH4HCO3, pH 10
MeOH with 10 mM NH4HCO3, pH 10
5 to 95% B in 3 min
0.4 mL/min
10 L
MRM
266.9 > 144.9
20.0
0.0
0.25
120.0
Silica-Based WCX (CBA) 10 mg, 96-well Plate
MRM
306.1 > 218.9
Recovery (%)
100.0
80.0
Protriptyline (H)
60.0
Valethamate (Q)
40.0
Atenolol (P)
20.0
0.0
Atenolol 10 ng/mL
101% Recovery
1.00
Loading volume (mL)
(b)
Instruments:
Waters 2777 Sample Manager,
and Waters 1525 binary HPLC pump,
Waters Quattro Ultima
(ESI in MRM)
0.25
MRM
264 > 191.1
Valethamate 10 ng/mL
106% Recovery
Protriptyline 10 ng/mL
102% Recovery
1.00
Loading volume (mL)
SPE recoveries for three types of bases versus volume of spiked saline solution loaded onto
(a) the Oasis WCX sorbent and (b) a commercially available silica-based WCX sorbent.
H is hydrophobic base, Q is quaternary amine, and P is polar base.
www.waters.com
LC/MS/MS chromatograms of the analytes (polar base, quaternary amine, and hydrophobic base)
extracted from rat plasma with Oasis WCX 96-well Elution plate. The SPE recoveries were
calculated by comparison with postextracted spiked plasma samples.
21
SaMPLE PREPaRaTION
Oasis WAX for Strong Acidic Compounds
High Selectivity and Sensitivity for Strong Acidic Compounds
Generic Oasis WAX Method for

Strong Acidic Compounds

Condition:
CH3OH
Equilibrate:
H2O
WAX sorbent
Weak Anion eXchange
+
NH
SO3
Load:
Spiked and acidified sample
+
NH 2
Wash 1:
2% HCOOH in H2O
Weak anion-exchange
interaction
Elute 1(Wash 2):

CH3OH
Reversed-phase
interaction
Elute 2:
5% NH4OH in CH3OH
Piperazine-anion-exchange
capacity: 0.6 meq/g

20% CH3OH in H2O
T he Oasis WAX (Weak Anion-eXc hange) SPE material was developed to

provide sample preparation for strong acidic compounds. T he retention
mechanism is mixed mode, both ion exchange and reversed phase, which
improves retention for strong acidic compounds.
Recovery Data for T hree Acidic Compounds from
Oasis WAX 1 cc, 30 mg Cartridge
Excellent Recovery for Strong Acid: Camphorsulfonic Acid
100
90
% Recovery
80
70
2-Naphthalenesulfonic
acid
60
Ketoprofen
SunFire C18 2.1 x 20 mm IS, 3.5 m

H2O with 10 mM CH3COO-NH4+, pH 7
MeOH with 10 mM CH3COO-NH4+, pH 7
0.4 mL/min
Time
Profile
(min)
%A
%B
0.0
95
5
3.0
5
95
4.0
5
95
4.1
95
5
5.0
95
5
10 L
Ambient
Waters 2777 Sample Manager,
Waters 1525 Binary HPLC Pump
and Waters Quattro Premier
Salicylic Acid
50
40
HPLC Conditions:
Column:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
Saline. pH = 7
Saline, pH = 1.3
pH 1.3 Rat Plasma
SPE recoveries for three types of acidic compounds spiked in saline pH 7

and 1.3, and in rat plasma pH 1.3.
Injection Volume:
Temperature:
Instrument:
100
100
2-Naphthalenesulfonic
acid
% Recovery
60
Ketoprofen
40
Salicylic Acid
20
DEA
PSA
Oasis
WAX
1 cc/
1 cc/
1 cc/ 100 mg 100 mg
30 mg
Oasis
WAX
1 cc/
30 mg
DEA
PSA
1 cc/
1 cc/
100 mg 100 mg
22
O
CH2SO3H
(1S)-Camphor-10-sulfonic acid
MW 232.3, C10H16O4S
Cone (V)
60
CID (eV)
30
108.8% recovery
%
1.29
2.75 3.10
1
100
1.59
Spiked Saline,
pH = 7
SPE recoveries for three types of acidic compounds versus volume of spiked
saline solution and rat plasma loaded onto (a) the Oasis WAX sorbent and
(b) a commercially available silica-based WAX sorbent.
CH3
Concentration: 10 pg/L
Recovery at Elution Step
80
1.59
150 C
350 C
50 L/Hr
600 L/Hr
2.2e-3 bar (Ar gas)
CH3
MRM Transition
m/z 231.1 _ 79.8
Recovery Data of T hree Acidic Compounds for

Oasis WAX vs. Silica-Based WAX Products
Breakthrough at Load Step
MS/MS Conditions:
Waters Quattro Premier
ESISource Temp:
Desolvation Temp:
Cone Gas Flow:
Desolvation Gas Flow:
Collision Cell:
4.20
4.33
Concentration: 50 pg/L
114.4% recovery
0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5 min
www.waters.com
SaMPLE PREPaRaTION
Oasis HLB for Reversed-Phase SPE
This generic, 1-D method (1-Dimensional only the organic strength is
changed) has proven useful for a wide variety of compound types and may
be the only protocol required, reducing method development time.
O
Recommended Generic Oasis HLB

SPE Method (1-D)*
CH
+
CH 2 N
SO3
SO3
Condition:
1 mL CH3OH
R+
CH
Equilibrate:
1 mL H2O
Load:
1 mL spiked sample**
Oasis HLB Sorbent

Oasis HLB sorbent makes it fast and easy to develop sample preparation
methods that deliver high, reproducible recoveries especially suited to
LC/MS/MS analysis by providing the required selectivity and sensitivity.
A Simple and Fast Generic SPE Reversed-Phase Protocol for Rapid
Method Development for a Wide Range of Compounds (1-D)
Limitations of traditional silica-based sorbents make you evaluate several
different bonded phases and brands to obtain acceptable results. With Oasis
HLB, acidic, basic, and neutral compounds, whether polar or nonpolar, can
be isolated reproducibly (RSDs <5%) with high recovery (>85%), using the
same simple SPE protocol (see below).
Wash (Mild Wash):

1 mL 5% CH3OH in H2O
Elute (Strong Elution):
1 mL CH3OH
40 C/under nitrogen stream
200 L mobile phase
* Volumes are given for the Oasis
HLB cartridge 1 cc/30 mg
Sample Pretreatment Suggestion

Applying one or more of the following
steps before loading your sample may
improve your results:
Dilute sample 1:1 with buffer
to improve flow during loading
** Add 20 L (H3PO4) to disrupt

drug-protein interaction/ binding
Dilute 1:1 or greater with
0.1 N HCl or other acids
Filter through 0.45 m membrane
Centrifuge @ 3000 rpm
One Simple Procedure: Many Applications on a Universal SorbentReduced Methods Development Time
100
sed sorbents
With silica-ba
need several
(C18) you would
istries to
em
ch
t
differen
ults
ac hieve the res
shown here
Acids
Neutrals
Bases
80
n=6
RSDs < 3.5%
% Recovery
60
1-D Method
40
Seventeen drugs with a wide range of polarities

were spiked in porcine serum, then extracted
with 1 cc/30 mg Oasis HLB cartridges, using
the same SPE method.
20
(20 L/mL H2PO4 was used to disrupt

drug-protein binding)
Ibu
pr
of
Na en
pr
ox
Sa
en
lic
yl
ic
Ac
Su
id
lfa
Su diaz
lfa
i
m ne
er
Ac
az
in
et
e
am
in
op
he
Th
n
eo
br
Pa omi
ra ne
za
nt
hin
Th
e
eo
ph
yl
lin
Ca e
ffe
Pr
in
oc
e
ain
am
i
Ra de
ni
tid
Ox ine
yc
od
on
Pr
e
op
ra
no
Na lol
ltr
ex
on
Sa
e
lb
ut
am
ol
Do
xe
pin
www.waters.com
23
SaMPLE PREPaRaTION
Oasis 2x4 Methodology
Oasis 2x4 Method
T he Oasis 2x4 Method is a simple, logical approach to the

selection of an SPE sorbent and protocol. Two protocols and
four sorbents provide the flexibility to extract acids, bases,
and neutrals with high SPE recoveries while removing
matrix components that may interfere with analysis.
Only 2 protocols and 4 sorbents to

analyze all types of compounds:
acids, bases, and neutrals
Follow the simple steps outlined in this flow c hart to

ac hieve high recoveries and the cleanest extracts:
Characterize your analyte [Neutral,

Acid, or Base, pKa]
Select one of the four Oasis sorbents
Apply the indicated Protocol [1 or 2]
Determine SPE recoveries by LC analysis
For Bases
pKa 2-10:
Use Oasis MCX
For Strong Acids

pKa <1:
Use Oasis WAX
For Acids
pKa 2-8:
Use Oasis MAX
Apply Protocol 1
Apply Protocol 2
Prepare Sample
Prepare Sample
Condition/Equilibrate
Load Sample
Load Sample
Wash:
2% HCOOH
Wash:
5% NH4OH
Elute 1:
100% CH3OH
Weaker
Acids
Base
Choosing Optimum Sorbent and Protocol for Neutral Compounds

Example: Prednisone in Plasma
SPE Recovery
50
100%
3.26
3.16
2.52
2.60
3.08
Oasis MCX Protocol 1
100
Protein
Precipitation
2.50
Oasis MAX Protocol 2
100
2.522.60
Oasis WAX Protocol 1

2.50
2.60
100
Oasis WCX Protocol 2

2.52
2.60
100
1.75
2.00
2.25
2.50
2.75
3.00
3.25
1: MRM of 5 Channels ES+

TIC
2.55e7
2% Phospholipids

TIC
2.55e7
9% Phospholipids

TIC
2.55e7
11% Phospholipids

TIC
2.55e7
12% Phospholipids
3.75
4.00 Min
Recovery is high on all four Oasis sorbents, but removal of

phospholipids, a primary cause of matrix ion suppression,
is best on Oasis MCX. Protein precipitation is a poor choice
for sample preparation prior to LC/MS/MS analysis.
Strong Acid
Acid
Strong Base
Oasis sorbent selection plate and cartridge kits enable rapid development
of SPE methods for LC/MS analysis. Having all four Oasis ion-exc hange
sorbents [MCX, MAX, WAX, and WCX] in a single plate is convenient for
scouting the best ways to accomplish efficient isolation of unknowns,
zwitterionic compounds, or mixtures of analytes with different retention/
elution properties.
100% Phospholipids

TIC
2.55e7
3.50
Elute 2:
2% HCOOH in CH3OH
Monitoring 5 Phospholipid
MRM Transitions
Cleanliness of 100% MeOH Elute 1

100
Weaker
Bases
Elute 1:
100% CH3OH
Neutrals
Elute 2:
5% NH4OH in CH3OH
Note that neutral analytes can be isolated from any of the four sorbents
in the Elute 1 step of either protocol. Choose the particular ion-exchange
sorbent that is best at removing specific matrix interferences. A good
example of this is shown below.
For Strong Bases

pKa >10:
Use Oasis WCX
OH
H
H
OH
Oasis Sorbent Selection Plate:

Evaluating Oasis 2x4 Method for Cephalexin
0
Prednisone
100%
Oasis MCX
Protocol 1 Elute 2
Oasis WAX
Protocol 1 Elute 1
SPE Recovery
50
Oasis MAX
Protocol 2 Elute 2
Oasis WCX
NH
pKa = 7.3
H H
N
H
O
N
HO
pKa = 2.6
Cephalexin
[Cephalosporin antibiotic]
A difficult amphoteric analyte
Protocol 2 Elute 1
Aliquots of prepared sample processed using Oasis 2x4 Method protocol designated for each
of 4 sorbents. Eluates from Elute 1 and Elute 2 steps analyzed by LC/MS/MS.
Clearly, Oasis MCX is the sorbent of choice.
24
www.waters.com
SaMPLE PREPaRaTION
Oasis 2x4 Method Proof-of-Concept

To demonstrate the logic, simplicity, and effectiveness of the Oasis 2x4
Method, five rat plasma samples were prepared, each containing one of
these characterized test analytes:
Imipramine, a base pKa of conjugate acid = 9.4
Ibuprofen, an acid pKa = 5.2
Decanesulfonic acid, a strong acid pKa < 0.5
Valethamate, a quaternary amine [strong base]

pKa of conjugate acid > 12
T he neutral analyte was processed on all four sorbents, as shown on the

previous page. Of the four method options, Oasis MCX with Protocol 1
proved superior at removing nearly all the phospholipids, eliminating this
major source of matrix effects, a known cause of ion suppression, loss of
sensitivity, and inaccurate quantitation in LC/MS analysis.
Prednisone, a neutral compound
Each plasma sample was diluted [1:1, v/v] and acidified with phosphoric acid
[4% in water]. Respective aliquots were then processed using the protocol
Oasis 2x4 Method Test on MCX: Base Isolation
Essentially, quantitative recovery and excellent cleanup efficiency were

ac hieved for eac h of t he ionic or ionizable test analytes w hen t he
recommended Oasis 2x4 Method sorbent/protocol combination was used.
T hese results are shown in the four figures below.
Oasis 2x4 Method Test on WAX: Strong Acid Isolation

For Bases
pKa 2 1 0 :
Use Oasis MCX
Oasis MCX
and the Oasis ion-mixed-mode sorbent designated by the Oasis 2x4 Method
for the corresponding sample type. LC/MS/MS analysis was used to
determine SPE recoveries.
For Strong Acids

pKa < 1:
Use Oasis WAX
Oasis WAX
Protocol 1
Protocol 1
Imipramine
Decanesulfonic
acid
SPE Recovery: 108%

n=4, 2-18% RSD
SPE Recovery: 94%

n=8, 3-6% RSD
pKa <1
3.19
2.71
pKa ~6
MRM ES+
281.2 > 85.95
4.16e6
Imipramine [pKa = 9.4]
5 Min.
Oasis 2x4 Method Test on MAX: Acid Isolation
Decanesulfonic acid [pKa <0.5]
O Na
5 Min.
Oasis 2x4 Method Test on WCX: Strong Base Isolation

For Acids
pKa 28:
Use Oasis MAX
Oasis MAX
MRM ES+
221.1 > 79.8
2.85e3
pKa ~18
For Strong Bases

pKa > 10:
Use Oasis WCX
Oasis WCX
Protocol 2
Protocol 2
Ibuprofen
Valethamate
SPE Recovery: 100%

n=6, 6-13% RSD
SPE Recovery: 106%

n=4, 9-13% RSD
pKa ~5
2.63
2.71
O
MRM ES+
205 > 161
1.34e4
OH
Br
MRM ES+
306.2 > 163
2.10e6
O
Ibuprofen [pKa = 5.2]
www.waters.com
5 Min.
Valethamate [pKa >12]
5 Min.
25
SaMPLE PREPaRaTION
Oasis Elution Plates
Waters Innovative SPE Technology
Elute in as little as 25 L
No evaporation and reconstitution
Ideal for small sample volumes
Up to a 15x increase in concentration
Now you can confidently perform SPE cleanup and analyte enrichment of
very small sample volumes (10-25 L) up to a maximum of 375 L. T he
Waters Oasis Elution plate combines patented plate design, proven Oasis
chemistries, and straightforward protocols that deliver high analyte recovery
and clean extracts in elution volumes as low as 25 L.
Reproducible elution in only 25 L enhances and streamlines SPE methods.

SPE can be performed on very small sample volumes. Sample enrichment
increases as much as 15-fold. A time-consuming step for eluate concentration by evaporation is no longer neededneither is evaporation/reconstitution if the eluting solvent is properly chosen to be compatible with the LC/
MS mobile phase. T he innovative features of Oasis Elution plates enable
sensitive, robust, reproducible results without evaporation and reconstitution.
Analytical performance, in terms of sensitivity, selectivity, and ruggedness,
is superior.
Comparison of Elution vs. Disk Technology
Elution Technology
Disk Technology
Wide and Short

Sorbent Bed
Narrow and Tall

Sorbent Bed
Ratio Bed H/D = 1.15
Ratio Bed H/D = 0.13
Vacuum
Vacuum
Oasis Elution Technology vs. Disk Technology

T here are two predominant low-elution SPE technologies in the marketplace today: SPE Disk and Oasis Elution. T hese technologies differ greatly
in performance primarily because of three features: aspect ratio, holdup
volume and elution volume.
A small holdup volume is necessary to minimize elution volume. SPE disks

have holdup volumes in the 3565 L range, compared to that for an Oasis
Elution bed plus frits which measures as little as 15 L. Having 24x less
holdup volume minimizes elution volume and reduces analyte loss during
elution, improving both recovery and precision.
Minimizing SPE elution volume is critical for SPE performance, recovery, and precision. Normal elution volume range for an Oasis Elution
plate is only 2550 L, far lower t han t he 75300 L typical for
SPE disk tec hnology.
26
Recovery Comparison of Elution vs. Disk Technology

T he innovative features of the Oasis Elution plates enable sensitive,
robust, reproducible results without evaporation and reconstitution.
Oasis Elution Technology
Disk Technology
>85% recovery in 25 L
25 L
Minimum of 100 L needed for best result
75 L
75 L
75 L
100
Elution Volume
80
% Recovery
T here is a correlation between a sorbent beds aspect ratio [ height to

diameter, H/D] and its performance in an SPE device. An H/D ratio less
than 1.0 often compromises extraction efficiency. In an SPE disk design
(e.g. membrane/glass-fiber disk), a small mass of sorbent is embedded in a thin support structure having good flow properties but a small
aspect ratio, H/D=0.13. In contrast, the Oasis Elution tec hnology uses
an internally tapered well, packed with high capacity Oasis sorbent,
having an aspect ratio, H/D=1.15, nearly 9x that of SPE disk tec hnology. An Oasis Elution plate well functions more like a c hromatography
column, minimizing loss of analyte during critical steps in the SPE
procedure and enhancing overall extraction efficiency.
60
40
20
0
Oasis HLB
Brand E C18
Brand E UR
Brand S C18
Elution plate
100 L Spiked Saline

acetaminophen
practolol
N-acetylprocainamide
betamethasone
caffeine
naproxen
amitriptyline
propranolol
www.waters.com
SaMPLE PREPaRaTION
Oasis 2x4 Method Optimized for Elution Plate
For Bases
pKa 2-10:
Use Oasis MCX
For Strong Acids

pKa <1:
Use Oasis WAX
For Acids
pKa 2-8:
Use Oasis MAX
For Strong Bases

pKa >10:
Use Oasis WCX
Apply Protocol 1
Apply Protocol 2
Prepare Sample
Prepare Sample
Load Sample
Load Sample
Wash:
2% HCOOH
Wash:
5% NH4OH
Elute 1:
100% CH3OH
Elute 2: 5% NH4OH in
60:40 CH3CN:CH3OH
Base
Elute 1:
100% CH3OH
Neutrals
Strong Acid
T he proven Oasis 2x4 method elution solvent is optimized to accommodate

the elutropic requirement of the small elution volume. Methanol is good as
a generic elution solvent, but is often not strong enough for 25 L elution
volumes. T he elution solvent recommended to be used with the Elution
plate must possess a high enough elutropic strength to fully elute analytes
in small volumes, and be appropriate for a diverse set of analytes.
T he recommended elution solvent for the Oasis 2x4 method optimized for
the Elution plate format is 60% CH3CN:40% CH3OH with a modifier. T his
was chosen as a starting point as it meets all of the above criteria.
Follow the simple steps outlined in this flow chart to achieve high recoveries
and the cleanest extracts:
Elute 2: 2% HCOOH in
60:40 CH3CN:CH3OH
Acid
Strong Base
Characterize your analyte [Neutral, Acid or Base, pKa].
Select one of the four Oasis sorbents.
Apply the indicated Protocol [1 or 2].
Determine SPE recoveries by LC analysis.
Oasis 2x4 Method Proof-of-Concept

Recovery Study:
Analytes Spiked into Rat Plasma
To demonstrate the logic, simplicity, and effectiveness of the Oasis 2x4

method, five samples of rat plasma were prepared, each spiked with one of
the previously characterized test analytes shown below:
O
N
O
O
H
Imipramine (B)
pKa = 9.4, 2 ng/mL
Ibuprofen: pKa = 5.2 (Acid)

Valethamate: pKa >12 (Quaternary Amine)
O F
HO
Nonafluoropentanoic Acid: pKa <0.5 (Strong Acid)
F F F F
Prednisone (N)
10 g/mL
OH
F
F
Nonafluoropentanoic Acid
(SA) pKa <0.5, 200 ng/mL
Prednisone: Neutral
Ibuprofen (A)
pKa = 5.2, 200 ng/mL
Protocol 1
Protocol 2
Oasis MAX
Oasis WCX
Pre
Oasis WAX
Pre
Oasis MCX
Valethamate (QA)
pKa >12, 2 ng/mL
OH
OH
Imipramine: pKa = 9.4 (Base)
100
80
% SPE Recovery
Each plasma sample was diluted [1:1, v:v] and acidified with phosphoric
acid [4% in water]. Respective aliquots were then processed using the
protocol and the Oasis ion-mixed-mode sorbent designated by the Oasis
2x4 Method for the corresponding sample type. LC/MS/MS analysis was
used to determine SPE recoveries. T he neutral analyte was processed on
all four sorbents used.
Br
N+
60
40
20
0
)
(sb
ate
ham
let
(n)
Va
ne
iso
dn
(n)
ic
no
nta
pe
(a)
fen
pro
(n)
Ibu
ne
iso
dn
oro
id
Ac
)
(sa
Elute 2: 60:40 CH3CN/CH3OH + modifier
www.waters.com
flu
ne
iso
dn
na
No
Pre
(b)
ine
am
i pr
(n)
Im
ne
iso
dn
Pre
Elute 1: CH3OH
Sample Preparation
27
SaMPLE PREPaRaTION
Elution Plate Loading Capacity
SPE Protocol for Oasis MCX Elution 96-Well Plate
SPE device capacity is defined as the total mass of analytes and endogenous
sample components retained by the sorbent bed under loading conditions.
Breakthrough will occur when the capacity of the sorbent bed is exceeded.
T he physicochemical properties of Oasis sorbents are designed to provide
exceptionally high loading capacity, even though each well in a Waters Oasis
Elution plate contains only 2 mg of Oasis sorbent.
Condition: 200 L CH3OH

Equilibrate: 200 L H2O
Load:
Various volumes of:
- Plasma diluted 1:1 with 4% H3PO4 in H2O
- Urine diluted 1:1 with H2O
To determine the Oasis Elution plate capacity, increasing volumes of

plasma and urine samples (from 50 L to 350 L in 50 L increments)
were spiked with 200 ng/mL imipramine (non-polar base) and 200 ng/
mL atenolol (polar base). T he plasma aliquots were diluted 1:1 with 4%
aqueous H 3PO 4 and the urine aliquots were diluted 1:1 with H 2O and
then loaded onto the Elution plate. SPE recovery was calculated and
plotted for eac h loading level.
Wash 1: 200 L 2% CHOOH in H2O

Wash 2: 200 L CH3OH
Elute: 2 x 25 L 5% NH4OH in
60:40 CH3CN:CH3OH
Dilute: 50 L H2O
Inject: 5 L
SPE Recovery for 200 ng/mL Imipramine and 200 ng/mL Atenolol on Oasis MCX Elution Plate
n=8
100
100
80
80
60
60
40
40
20
20
50
100 150
200 250 300
350 L
Matrix Effects for Polar and

Non-Polar Analytes in Plasma
Ion Suppression Ion Enhancement
Imipramine
(non-polar)
SPE Recovery for Polar and

Non-Polar Analytes in Urine Example
% Recovery
Atenolol (polar)
% Recovery
SPE Recovery for Polar and

Non-Polar Analytes in Plasma Example
50
Loaded Plasma Volumes (Undiluted)
100 150
200 250 300
350 L
Loaded Urine Volumes (Undiluted)
40
30
20
10
0
-10
-20
-30
-40
50
100 150 200 250 300
350 L
Loaded Plasma Volumes (Undiluted)
Oasis 96-Well High-T hroughput Extraction Plates

Versatile, High-T hroughput Oasis 96-Well Extraction Plates
analytes, including polar and basic compounds, with RSDs less than
5% (n=96).
Waters 96-Well Plate Design
1999 R&D
100 Award
Two-Stage
Well Design
Waters award-winning plate design, with five chemistry and four sorbentmass options, provides flexible high-throughput SPE in a single device.
T he Oasis 96-well plates are designed to be used on many manifold
configurations and most robotic liquid handling systems. Oasis sorbents
unique balance of hydrophobicity and water-wettability means you will
never have to worry about poor results if individual wells of the 96-well
plate dry out. As always, you can expect Oasis SPE products to perform
reliably, delivering high and reproducible recoveries for a wide range of
28
Sample Preparation
5 mg
10 mg
30 mg
60 mg
By varying frit size and/or placement, the same plate may be filled with various quantities of sorbent per well.
Our design permits optimal recoveries, even with low sorbent weights for smaller elution volumes.
www.waters.com
SaMPLE PREPaRaTION
Speed and T hroughput
Extraction Protocols
Comparison of Oasis Elution Plate and Protein

PrecipitationSuperior Results, Less Time and Effort
T he protein precipitation generic method includes both a centrifugation and

an evaporation step, which produces the cleanest sample extract possible
for protein precipitation. T he final sample volume (75 L) is the same
for all three generic methods. T he Oasis generic methods produce cleaner
extracts than protein precipitation, demonstrated by the improved sensitivity with the Oasis HLB (4X) and the Oasis MCX method (9X to 25X). T he
Oasis method enables improved sensitivity by eliminating matrix effect
and reducing ion suppression. Achieve superior results compared to protein
precipitation in less time using the Oasis Elution plate.
T he Oasis Elution plate optimizes the configuration of the highly efficient

Oasis sorbents (HLB, MCX, MAX, WCX, and WAX), enabling elution volumes
as low as 25 L, providing fast cleanup with improved performance over
protein precipitation. Time consuming evaporation and reconstitution steps
are eliminated, compressing preparation cycle time and increasing throughput capabilities.
Protein Precipitation
Generic Method
Oasis HLB Method
Add 50 L spiked plasma (1 to

1000 pg/L), 50 L IS (10 pg/L) in H2O
Condition with 200 L CH3OH
Condition with 200 L CH3OH
Equilibrate with 200 L H2O
Equilibrate with 200 L H2O
Load 50 L spiked rat plasma,

50 L IS (10 pg/L) in H2O
Load 50 L spiked rat plasma,

50 L IS (10 pg/L) in H2O
Wash with 200 L 5% CH3OH

in H2O
Wash 1 with 200 L H2O + 2% COOH
Oasis MCX Method
Oasis HLB Elution Plate*

Part Number186001828
Add 1 mL CH3CN to precipitate plasma

Centrifuge the plasma at 3600 rpm
for 30 minutes
Transfer the supernatant to another
2 mL 96-well container
Oasis MCX Elution Plate*

Part Number 186001830
Wash 2 with 200 L CH3OH
Elute with 25 L CH3CN:(CH3)2CHOH

40:60 + 2% HCOOH
Evaporate the solution to dryness

Reconstitute with 25 L CH3CN:(CH3)2CHOH
(40:60) + 2% NH4OH and 50 L H2O
Elute with 25 L CH3CN:(CH3)2CHOH

40:60 + 5% NH4OH
Dilute with 50 L H2O
Dilute with 50 L H2O
Inject 20 L
Inject 20 L
Inject 20 L
Increase in Selectivity (More Selective Methods)

* Tomtec Quadra 96 used.
Oasis HLB and MCX Elution Plate versus Protein Precipitation

LC Conditions
Column:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
LC Conditions:
Temperature:
LC Instrument:
MRM Transition:
MS Conditions
MS Instrument:
XTerra MS C18,
2.1 x 30 mm, 3.5 m
H2O + 0.5% NH4OH
ACN + 0.5% NH4OH
0.2 mL/min
5% - 95% B 1 min.
Room temperature
Waters Alliance 2795
Ion Source:
Source Temperature:
Gas Cell:
Desolvation Temperature:
Drying Gas Flow:
Cone Gas Flow:
Quattro Triple
Quadrupole
ESI (+)
150 C
2.0 e-3 bar Argon
350 C
500 L/ hr
50 L/ hr
Cone Voltage:
Collision Energy:
Capillary Voltage:
25 volts
20
3.5 Kv
Propranolol, 1 ng/mL
3.79
Intensity: 7.50 x103
4x sensitivity
without
evaporation
Oasis HLB
4.75
5.18
4.67
4x sensitivity
without
evaporation
4.48
9x sensitivity
without
evaporation
Oasis HLB
100
100
100
3.83
299.8 226.7
259.9 154.9
278.1 232.9
263.9 190.8
17
16
100
m/z
m/z
m/z
m/z
Amitriptyline, 0.1 ng/mL

100
100
Metoclopramide (IS)
Propranolol
Amitriptyline
Nortriptyline
3.83
Oasis MCX
Intensity: 1.90
25x sensitivity
without
evaporation
x105
2
0.5
www.waters.com
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0 min
Oasis MCX
0.5
1.0
Intensity: 8.91 x10

1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0 min
29
SaMPLE PREPaRaTION
Sensitivity and Precision
Comparison of Oasis MCX Elution Plate and Glass Fiber and
Membrane 96-well Disk PlatesUp to 18X Increase in Sensitivity
and glass fiber plates, which require a 150 L elution volume for complete
analyte recovery. T he Oasis Elution plate enables sensitivity gains and
delivers high performance without the time consuming evaporation step.
T he Oasis Elution plate shows superior performance when compared to

other low elution disk plate products used according to recommended
protocols. A generic Oasis MCX method for terfenadine with 50 L elution,
dilution and direct injection shows better sensitivity than the membrane
Oasis MCX Elution Plate vs. Glass Fiber and Membrane 96-well Disk Plates
Glass Fiber SCX, 96-well Plate
250 L plasma loaded
150 L elution
% %
2.5 2.5
3.0 3.0
2.0
2.542.54
100
S/N = 11
% %
-10-10
100100
Intensity:
3.03 x104
-10
2.0 2.0
2.5 2.5
2.702.70
100100
3.0 3.0
2.0
100
S/2 = 51
% %
-10-10
2.5 2.5
3.0 3.0
2.0
2.472.47
3.0
2.70
3.5 2.0
min2.0
3.0
2.47
100
2.632.63
% %
Final extract diluted 1:2 with H2O

Extracted using manufacturers recommended protocol
3.5 2.0
min2.0
2.5Min.
3.5 3.5
Min.
3.0
3.0 3.0
2.0
2.452.45
100
16x Sensitivity
% %
2.63
100
100100
Intensity:
1.15 x105
2.5min
3.5 3.5
min
3.0
3.5 3.5
min
2.5min
3.0
3.5 min
2.45
Intensity:
3.11 x105
S/N = 178
3.0 3.0
2.0
2.622.62
18x Sensitivity
% %
3.5 2.0
min2.0
Intensity:
1.14 x107
S/N = 357
-10
2.5 2.5
-10-10
-10
2.0
3.0 3.0
3.5 2.0
Min.2.0
2.92
-10
2.5 2.5
-10-10
2.0
3.0 3.0
2.5 2.5
6x Sensitivity
% %
100100
Intensity:
7.14 x104
S/N = 40
3.5 2.0
min2.0
3.0
-10
2.5 2.5
-10-10
3.5 3.5
min
2.5min
2.5min
3.5 3.5
min
100100
Intensity:
7.30 x104
2.0
3.0 3.0
S/N = 17
% %
2.922.92
100
100100
-10-10
-10
2.5 2.5
-10-10
3.5 3.5
min
2.5min
-10
2.0 2.0
3.5 2.0
min2.0
2.54
100100
Terfenadinecarboxylate
0.5 ng/mL
3.0
2.92
-10-10
3.5 3.5
min
2.5min
Oasis MCX Elution Plate

250 L plasma loaded
50 L elution
Intensity:
7.44 x106
S/N = 82
% %
-10
2.0 2.0
2.92
2.92
100
100100
Intensity:
5.75 x106
S/N = 61
-10-10
Terfenadinealcohol
0.5 ng/mL
3.02
3.023.02
100
100100
Terfenadine
0.5 ng/mL
Membrane MPC 96-well Plate

250 L plasma loaded
150 L elution
100
3.5 3.5
min
2.5min
3.0
3.5 min
2.62
Intensity:
5.04 x105
S/N = 927
-10
2.5 2.5
3.0 3.0
2.0

3.5 3.5
min
2.5min
3.0
3.5 min

Validation Assay of Terfenadine and Its Metabolites Using the Oasis MCX Elution Plate
30
Calibration range: 0.5-200 ng/mL, 7 levels
Six calibration curves per day over four days
Analyte recoveries over calibration range: 95%
Inter-day %RSD of back-calculated

standard concentrations over four
days (24 values) 5.6%
Intra-day %RSD of back-calculated

standard concentrations within one
day (6 values) 8.0%
www.waters.com
SaMPLE PREPaRaTION
Sorbent Amount and Solvent Selection for the Generic Method
The suggested amount of sorbent in a cartridge or a plate required for
your application, is given in the table below. Remember, because of the
increased capacity of the Oasis sorbents, you can use less sorbent than you
would normally need if you used a silica-based packing. The solvent used
for the elution step should be selected based on the polarity of the analyte.
The second table below gives a selection of elution solvents and each
solvent gives you different selectivity and elution strength.
Capacity and Elution Volume of Oasis
96-Well Plates and Cartridges
Sorbent
Per Device
Maximum
Mass Capacity
Typical
Sample Volumes
Elution
Volume
Elution Plate*
5 mg*
10 mg
30 mg
60 mg
60 to 400 g
0.15 to 1 mg
0.35 to 2 mg
1 to 5 mg
2 to 10 mg
10 to 375 L
10 to 100 L
50 to 200 L
100 L to 1 mL
200 L to 2 mL
25 L**
150 L
250 L
400 L
800 L
* Available only in 96-well plates

** Elution Plate requires no evaporation step
When converting from C18 silica-based to Oasis SPE sorbents, use approximately 2/3 less
Oasis sorbent (100 mg C18 sorbent = 30 mg Oasis sorbent).
Tips for Selecting Elution Solvents for the Generic SPE Method (1-D)*
The elution solvent is selected based on polarity of analyte.
Relative
Elution Strength**
Solvent
Solvent Type
Methanol
Acetonitrile
Tetrahydrofuran
Acetone
Ethyl Acetate
proton donor
dipole-dipole
dipole-dipole
dipole-dipole
dipole-dipole
1.0
3.1
3.7
8.8
high
Methylene Chloride
dipole-dipole
high
Comments
disrupts H-bonding
medium polarity drugs
nonpolar drugs and
GC compatible
nonpolar drugs and
GC compatible
* When using solvents other than methanol, add 10-30% (of proton donor solvent like
methanol) to disrupt H-bonding on the Oasis HLB sorbent.
** High-Purity Solvent Guide. Burdick & Jackson Laboratories, Inc. Solvent Properties of
Common Liquids, L.R. Snyder, J. Chromatogr., 92, 223 (1974); J. Chromatogr. Sci.
16, 223 (1978)
The generic method (1-D) is an excellent starting protocol for methods

development. If you were not able to meet all of your SPE goals, then the
advanced 2-D protocol will provide a rapid way to chromatographically
determine an even cleaner, more selective and sensitive result.
Note: Larger capacity cartridges are also available.
Automation
Automation of Oasis Sample Extraction Cartridges and 96-Well Plates
Oasis SPE products are compatible with the following liquid handling and/or SPE automation systems:
Perkin Elmer Robotic liquid

handling system, MultiProbe II
Beckman Biomek 2000 Laboratory

Automation Workstation
Tomtec Quadra3 and Quadra3SPE
Hamilton Microlab SPE Workstation
Caliper Life Science RapidTrace Automated

SPE Workstation, AutoTrace
Gilson ASPEC XL4
Gilson ASPEC XL
Gilson 215 SPE Liquid Handler
Tecan Genesis FE500
Spark Holland Symbiosis
Waters ACQUITY UPLC MS/MS

System for Bioanalysis Featuring
the Waters Xevo TQ MS
For high sensitivity analyses, suc h as those employing UPLC/MS/MS,
proper sample preparation can be critical for minimizing matrix effects and
concentrating analytes of interest. Oasis sample preparation can be used
with UPLC/MS/MS systems to provide the cleanest extracts.
T he system shown here integrates the Xevo TQ MS, a highly advanced
mass spectrometer, combining MassLynx informatics with innovative
ScanWave and IntelliStart tec hnologies, and t he Waters ACQUIT Y
UPLC system.
www.waters.com
31
SaMPLE PREPaRaTION
Oasis On-Line Columns
On-Line SPE Columns for LC/MS/MS
T here are three HPLC Oasis on-line column configurations designed to fit
all your on-line-analysis needs.
T he Oasis cartridge column fits into a Sentry holder that features a

finger-tight fitting for fast, convenient replacement1
T he Oasis direct-connect column can be screwed directly into a

switching valve or connected to fittings like those for a conventional
HPLC column2
T he Oasis column features traditional HPLC column

fittings and hardware3
All of these formats are available with the five Oasis patented sorbents
(HLB, MCX, MAX, WCX, and WAX) in a wide choice of particle sizes and
dimensions. T he Oasis on-line columns make it possible to analyze
a specific analyte in a sample matrix when combined with appropriate
Waters narrow-bore analytical columns (suc h as XSelect, XBridge,
SunFire, Atlantis , XTerra , or Symmetry columns).
On-Line System Configuration

Oasis
On-Line Column
Isocratic
HPLC
Waste
10 Port Valve
2 Positions
Gradient
HPLC
Mass
Spectrometer
Narrow Bore
Analytical Column
10
1
8
7
4
Waste
9
8
7
4
Alliance HT
Mass
Spectrometer
Mass
Spectrometer
10
Alliance HT
Analytical
Column
32
515
System
Oasis
Waste
INJECTION
POSITION
515
System
Oasis
LOAD
POSITION
Analytical
Column
www.waters.com
SaMPLE PREPaRaTION
Oasis Glass Cartridges for PPT Detection Levels
Waters Oasis glass cartridges are available in 5 cc (200 mg) configuration
with Teflon frits for trace analysis at parts per trillion level. Eac h lot is
tested for the presence of bisphenol A and other phenols and phthalates,
assuring that endocrine disruptors in water samples can be analyzed to part
per trillion levels.
Endocrine Disruptors
PPT Recovery of Estrogens from River Water,
LC/MS, 5 ng/L Spike Level, n=4
Results
Ions Monitored (m/z)
Recovery
% RSD
227
271
295
269
267
113
93
96
87
75
11
15
12
5
5
Recovery
% RSD
130
86
90
110
110
60
15
12
11
11
8
8
1. bisphenol A
2. 17b-estradiol
3. 17a-ethynylestradiol
4. estrone
5. diethylstilbestrol
Recovery of Phthalates and Nonylphenol from

River Water, GC/MS, 200 ng/L Spike Level, n=4
Results
1. dimethylphthalate
2. dirthylphthalate
3. n-nonylphenol
4. dibutylphthalate
5. benzylbutylphthalate
6. bis(ethylhexyl)phthalate
ISTD. o-terphenyl (internal standard)
See the full application in the Oasis Applications Notebook

available for download at www.waters.com/oasis
Oasis Symbiosis On-Line Cartridges

On-Line SPE with Oasis Sorbent in Symbiosis Cartridges
Waters Oasis sorbents are available in Symbiosis cartridges for use with
Spark Holland Symbiosis systems. Eac h narrow-bore PV DF cartridge
is available in either 1.0 x 10 mm or 2.0 x 10 mm format, and all five
patented Oasis sorbentsHLB, MCX, MAX, WCX, and WAX.
T he Oasis sorbents in Symbiosis cartridges show the same performance

advantages as in other Oasis formats for pharmaceutical compounds when
compared to silica-based sorbents.
Recovery Comparison: Oasis HLB vs. Bonded-Silica Symbiosis Cartridge
Oasis HLB
C18
C2
20
60
40
80
100
% Recovery
Clonidine
Terconazole
*Data provided by Dr. Francis Beaudry, Principal Research Scientist,

Phoenix International Life Sciences, Inc., Montreal, Quebec, Canada.
www.waters.com
33
SAMPLE PREPARATION
Oasis Product Selection Guide
1 cc/
10 mg
1 cc/
30 mg
1 cc/30 mg
Flangeless
1 cc/30 mg
Gilson Adapter
3 cc/
60 mg
3 cc/60 mg
Flangeless
3 cc/60 mg
Gilson Adapter
3 cc/
540 mg
3 cc/540 mg
Flangeless
100/box
100/box
100/box
500/box
100/box
100/box
500/box
100/box
100/box
Oasis HLB 30 m
186000383
WAT094225
186001879
WAT058882
WAT094226
186001880
WAT058883
Oasis HLB 60 m
186004134
186003852
Sorbent
Oasis MCX 30 m
186004648
186000252
186001881
186001888
186000254
186001882
Oasis MCX 60 m
186000782
186000253
Oasis MAX 30 m
186004649
186000366
186001883
186000367
186001884
Oasis MAX 60 m
186000368
Oasis WCX 30 m
186004650
186002494
186002495
Oasis WCX 60 m
186002496
186002497
Oasis WAX 30 m
186004651
186002489
186002490
Oasis WAX 60 m
186002491
186002492
Oasis 96-well Plates
Oasis 96-well Elution Plates
5 mg/
96-well
10 mg/
96-well
30 mg/
96-well
60 mg/
96-well
1/pk
1/pk
1/pk
1/pk
Oasis HLB 30 m
186000309
186000128
WAT058951
Oasis HLB 30 m
186001828BA
Oasis HLB 60 m
186000679
Oasis MCX 30 m
186001830BA
Oasis MCX 30 m
186000259
186000248
Oasis MAX 30 m
186001829
Oasis MCX 60 m
186000250
186000678
Oasis WCX 30 m
186002499
Oasis MAX 30 m
186000375
186000373
186001256
Oasis WAX 30 m
186002500
Oasis MAX 60 m
186001205
Oasis WCX 30 m
186002501
186002503
Oasis WAX 30 m
186002502
186002504
186003915
Description
34
Elution
96-well
Description
1/pk
www.waters.com
SaMPLE PREPaRaTION
6 cc/
150 mg
6 cc/
200 mg
6 cc/400 mg
Flangeless
6 cc/
500 mg
12 cc/
500 mg
20 cc/
1g
35 cc/
6g
Plus
225 mg
Vac RC
30 mg
Vac RC
60 mg
Glass Cartridge
5 cc/200 mg
30/box
30/box
100/500/box
30/box
20/box
20/box
10/box
50/box
50/box
50/box
30/box
186003365
WAT106202
186000382
186000381
186003379
186000115
186000116
186000117
186000118
186000132
186000683
186000256
186001216
186000261
186000255
186000776
186000777
186000778
186003516
186000380
186000369
186001855
186000372
186000371
186000370
186000865
186003517
186000378
186002498
186004646
186003518
186002493
186004647
186003519
Oasis Sorbent Selection Tools

The Oasis Sorbent Selection Tools enable the rapid
development of SPE-LC-MS-MS methods. T hese Sorbent
Selection Tools have all the Oasis sorbents, allowing
you the ability to extract your analyte of interest.
Description
Oasis Elution Sorbent Selection Plate, 96-well
Oasis Sorbent Selection Plate,10 mg/96-well
Oasis Sorbent Selection Kit, 30 mg/1 cc cartridge
www.waters.com
Particle Size
Part No.
30 m
30 m
30 m
186004475
186003249
186003463
35
SAMPLE PREPARATION
Oasis HLB Sample Extraction Products
Oasis MCX Sample Extraction Products (Cation Exchange)

Particle
Size
30 m
30 m
30 m
30 m
Qty.
Part No.
100/box
100/box
1000/box
100/box
186000383
WAT094225
186003908
186001879
30 m
500/box
186000988
30 m
500/box
WAT058882
30 m
30 m
100/box
100/box
WAT094226
186001880
30 m
500/box
WAT058883
30 m
60 m
60 m
60 m
30 m
60 m
60 m
60 m
60 m
60 m
60 m
30 m
30 m
30 m
60 m
30 m
30 m
30/box
100/box
100/box
100/box
30/box
30/box
30/box
20/box
20/box
10/box
50/box
50/box
50/box
50/box
30/box
96/box
96/box
48/box
12/box
10/box
10/box
10/pk
WAT106202
186003849
186004134
186003852
186003365
186003379
186000115
186000116
186000117
186000118
186000132
186005125
186000382
186000381
186000683
186001196
186003925
WAT011390
186005587
WAT054260
WAT048160
405000934
Oasis HLB column

2.1 x 20 mm
Oasis HLB column
3.0 x 20 mm
Oasis HLB column
3.9 x 20 mm
Oasis HLB cartridge column
3.9 x 20 mm
Oasis HLB column
4.6 x 20 mm
Oasis HLB column
2.1 x 20 mm
Oasis HLB column
3.0 x 20 mm
Oasis HLB column
3.9 x 20 mm
3.9 x 20 mm
Oasis HLB column
4.6 x 20 mm
Oasis HLB column
2.0 x 15 mm
Oasis HLB column
2.1 x 20 mm
2.1 x 20 mm
Oasis HLB column
3.0 x 20 mm
Oasis HLB column
3.9 x 20 mm
Oasis HLB column
4.6 x 20 mm
Holder kit for 2.1 x 20 mm cartridge column
Extraction column connector
Inline precolumn filter kit
Replacement filters
5 m
5 m
5 m
5 m
5 m
15 m
15 m
15 m
15 m
15 m
25 m
25 m
25 m
25 m
25 m
25 m
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
5/pk
186002034
186002037
186002040
186001413
186002043
186002035
186002038
186002041
186001414
186002044
186001792
186002036
186000706
186002039
186002042
186002045
186000262
WAT046910
WAT082745
WAT084560
WAT005139
Oasis HLB Elution plate

Oasis HLB plate
Oasis HLB plate
Oasis HLB plate
Oasis HLB plate
30 m
30 m
30 m
30 m
60 m
1/pk
1/pk
1/pk
1/pk
1/pk
186001828BA
186000309
186000128
WAT058951
186000679
Description
Oasis HLB cartridge
1 cc/10 mg
Oasis HLB cartridge
1 cc/30 mg
Oasis HLB cartridge
1 cc/30 mg
Oasis HLB flangeless cartridge
1 cc/30 mg
Oasis HLB cartridge with
1 cc/10 mg
Gilson ASPC adapter
1 cc/30 mg
Gilson ASPC adapter
Oasis HLB cartridge
3 cc/60 mg
3 cc/60 mg
3 cc/60 mg
Gilson ASPC adapter
Oasis HLB cartridge
6 cc/200 mg
Oasis HLB
3 cc/400 mg
Oasis HLB cartridge
3 cc/540 mg
3 cc/540 mg
Oasis HLB cartridge
6 cc/150 mg
Oasis HLB cartridge
6 cc/150 mg
Oasis HLB cartridge
6 cc/500 mg
Oasis HLB cartridge
12 cc/500 mg
Oasis HLB cartridge
20 cc/1 g
Oasis HLB cartridge
35 cc/6 g
Oasis HLB Plus cartridge
225 mg
Oasis HLB Light cartridge
30 mg
Oasis HLB Vac RC cartridge
20 cc/30 mg
Oasis HLB Vac RC cartridge
20 cc/60 mg
Oasis HLB glass cartridge
5 cc/200 mg
Oasis HLB Symbiosis cartridge*
1.0 x 10 mm
Oasis HLB Symbiosis cartridge*
2.0 x 10 mm
Reservoir 30 cc for Oasis cartridges
Reservoir 60 cc for Oasis cartridges
Reservoir adapter for 1 cc, 3 cc, 6 cc cartridges
Reservoir adapter for 12 cc, 20 cc, 35 cc cartridges
Reservoir adapter for 5 cc cartridges, Teflon
36
2 mg/96-well
5 mg/96-well
10 mg/96-well
30 mg/96-well
60 mg/96-well
Oasis MCX cartridge

Oasis MCX cartridge
Oasis MCX flangeless cartridge
Oasis MCX cartridge
Oasis MCX cartridge
Oasis MCX flangeless cartridge
Oasis MCX cartridge
Oasis MCX cartridge
Oasis MCX cartridge
Oasis MCX cartridge
Oasis MCX cartridge
Oasis MCX cartridge
Oasis MCX Plus cartridge
Oasis MCX Vac RC cartridge
Oasis MCX Vac RC cartridge
Oasis MCX Symbiosis cartridge
1cc/10mg
1 cc/30 mg
1 cc/30 mg
1 cc/60 mg
3 cc/60 mg
3 cc/60 mg
3 cc/60 mg
6 cc/150 mg
6 cc/150 mg
6 cc/500 mg
20 cc/1 g
35 cc/6 g
225 mg
20 cc/60 mg
20 cc/60 mg
10 x 1 mm
Particle
Size
30 m
30 m
30 m
60 m
30 m
30 m
60 m
30 m
60 m
60 m
60 m
60 m
60 m
30 m
60 m
30 m
Oasis MCX column

Oasis MCX cartridge column
Oasis MCX column
Oasis MCX column
Oasis MCX column
2.1 x 20 mm
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
4.6 x 20 mm
30 m
30 m
30 m
30 m
30 m
1/pk
1/pk
1/pk
1/pk
1/pk
186002046
186002051
186002047
186002048
186002049
Oasis MCX Elution plate

Oasis MCX plate
Oasis MCX plate
Oasis MCX plate
Oasis MCX plate
96-well
10 mg/96-well
30 mg/96-well
30 mg/96-well
60 mg/96-well
30 m
30 m
30 m
60 m
60 m
1/pk
1/pk
1/pk
1/pk
1/pk
186001830BA
186000259
186000248
186000250
186000678
Description
Qty.
Part No.
100/box
100/box
100/box
100/box
100/box
100/box
100/box
30/box
30/box
30/box
20/box
10/box
50/box
50/box
50/box
96/box
186004648
186000252
186001881
186000782
186000254
186001882
186000253
186000256
186000255
186000776
186000777
186000778
186003516
186000261
186000380
186002098
* For use with Spark Holland Symbiosis systems
Literature
References

Waters Positive Pressure-96
Processor Brochure,
Bioanalysis Brochure,
www.waters.com
SAMPLE PREPARATION
Oasis MAX Sample Extraction Products (Anion Exchange)
Oasis MAX cartridge

Oasis MAX cartridge
Oasis MAX flangeless cartridge
Oasis MAX cartridge
Oasis MAX cartridge
Oasis MAX flangeless cartridge
Oasis MAX cartridge
Oasis MAX cartridge
Oasis MAX cartridge
Oasis MAX Plus cartridge
Oasis MAX Vac RC cartridge
Oasis MAX Symbiosis cartridge*
1 cc/10 mg
1 cc/30 mg
1 cc/30 mg
3 cc/60 mg
3 cc/60 mg
3 cc/60 mg
6 cc/150 mg
6 cc/150 mg
6 cc/500 mg
225 mg
20 cc/30 mg
20 cc/60 mg
20 cc/60 mg
10 x 1 mm
Particle
Size
30 m
30 m
30 m
30 m
60 m
30 m
30 m
60 m
60 m
60 m
30 m
30 m
60 m
30 m
Oasis MAX column

Oasis MAX cartridge column
Oasis MAX column
Oasis MAX column
Oasis MAX column
2.1 x 20 mm
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
4.6 x 20 mm
30 m
30 m
30 m
30 m
30 m
Description
Oasis MAX Elution plate

Oasis MAX plate
Oasis MAX plate
Oasis MAX plate
Oasis MAX plate
2 mg/96-well
10 mg/96-well
30 mg/96-well
60 mg/96-well
60 mg/96-well
Qty.
Part No.
100/box
100/box
100/box
100/box
100/box
100/box
30/box
30/box
30/box
50/box
50/box
50/box
50/box
96/box
186004649
186000366
186001883
186000367
186000368
186001884
186000369
186000370
186000865
186003517
186000372
186000371
186000378
186002099
1/pk
1/pk
1/pk
1/pk
1/pk
186002052
186002057
186002053
186002054
186002055
1/pk
1/pk
1/pk
1/pk
1/pk
30 m
30 m
30 m
60 m
Oasis WCX Sample Extraction Products (Weak Cation Exchange)
186001829
186000375
186000373
186001256
186001205
* For use with Spark Holland Symbiosis systems
Oasis Method Development Kits

Particle
Size
Part No.
96-well
30 m
186003249
96-well
30 m
186004475
1 cc/30 mg
30 m
186003463
Description
Oasis sorbent selection plate
3 rows each:
MCX, MAX, WCX and WAX
Oasis Elution Sorbent
Selection Plate, 3 rows each:
Oasis sorbent selection
cartridge kit, 10 each:
Oasis WCX cartridge

Oasis WCX cartridge
Oasis WCX cartridge
Oasis WCX cartridge
Oasis WCX cartridge
Oasis WCX cartridge
Oasis WCX cartridge
Oasis WCX Plus cartridge
Oasis WCX Elution plate
Oasis WCX 96-well plate
Oasis WCX 96-well plate
Oasis WCX Symbiosis cartridge*
1 cc/10 mg
1 cc/30 mg
3 cc/60 mg
6 cc/150 mg
1 cc/30 mg
3 cc/60 mg
6 cc/500 mg
225 mg
2 mg/96-well
10 mg/96-well
30 mg/96-well
1.0 x 10 mm
Particle
Size
30 m
30 m
30 m
30 m
60 m
60 m
60 m
60 m
30 m
30 m
30 m
30 m
Oasis WCX column

Oasis WCX column
2.1 x 20 mm
3.9 x 20 mm
30 m
30 m
Description
Qty.
Part No.
100/box
100/box
100/box
30/box
100/box
100/box
30/box
50/box
1/pk
1/pk
1/pk
96/box
186004650
186002494
186002495
186002498
186002496
186002497
186004646
186003518
186002499
186002501
186002503
186002892
186002505
186002507
Oasis WAX Sample Extraction Products (Weak Anion Exchange)
Oasis WAX cartridge

Oasis WAX cartridge
Oasis WAX cartridge
Oasis WAX cartridge
Oasis WAX cartridge
Oasis WAX cartridge
Oasis WAX cartridge
Oasis WAX Plus cartridge
Oasis WAX Elution plate
Oasis WAX 96-well plate
Oasis WAX Symbiosis cartridge*
1 cc/10 mg
1 cc /30 mg
3 cc /60 mg
6 cc /150 mg
1 cc /30 mg
3 cc /60 mg
6 cc/500 mg
225 mg
96-well
10 mg/96-well
30 mg/96-well
60 mg
1.0 x 10 mm
Particle
Size
30 m
30 m
30 m
30 m
60 m
60 m
60 m
60 m
30 m
30 m
30 m
30 m
30 m
Oasis WAX column

Oasis WAX column
2.1 x 20 mm
3.9 x 20 mm
30 m
30 m
Description
Qty.
Part No.
100/box
100/box
100/box
30/box
100/box
100/box
30/box
50/box
1/pk
1/pk
1/pk
1/pk
96/box
186004651
186002489
186002490
186002493
186002491
186002492
186004647
186003519
186002500
186002502
186002504
186003915
186002893
186002508
186002509
NEW PST T herapeutic Peptide Method Development Kits

Description
For more details, see page 209
www.waters.com
Part No.
UPLC PST Therapeutic Peptide Method Development Kit

Includes:
1 x Oasis PST Elution Method Development Plate, 186004713
1 x ACQUITY UPLC BEH 300 C18 2.1 x 50 mm, 1.7 m, 186003685
3 x 96-well 1 mL Collection Plate and Cap Mat, 600001043
176001835
HPLC PST Therapeutic Peptide Method Development Kit

Includes:
1 x Oasis PST Elution Method Development Plate, 186004713
1 x XBridge BEH 300 C18 2.1 x 50 mm, 3.5 m, 186003607
3 x 96-well 1 mL Collection Plate and Cap Mat, 600001043
176001836
37
SAMPLE PREPARATION
Accessories for Extraction Cartridge Manifold
Manifold for Extraction Plate

Description
Qty.
Extraction plate manifold for Oasis 96-well plates

Extraction plate manifold kit A
(includes extraction plate manifold, reservoir tray,
sealing cap and 350 L sample collection plate)
Extraction plate manifold kit B
(as kit A, with 1 mL sample collection plate)
Extraction plate manifold kit C
(as kit A, with 2 mL sample collection plate)
1/box
Part No.
186001831
WAT097944
WAT097945
WAT097946
Oasis 96-well Plate

(No Spacer Required)
Extraction Plate
Manifold
186001831
Collection Plate
Accessories for Extraction Plate Manifold

Description
Disposable reservoir tray
Sample collection plate, 350 L
Sample collection plate, 2 mL
Sealing cap for 96-well collection plate
SPE vacuum pump 115 V 60 Hz
Vacuum box gasket kit
Kit includes: 2 foam top gaskets, 2 orange O-rings
30 cc Reservoir
60 cc Reservoir
Reservoir Adapters for 1, 3 and 6 cc VAC
Reservoir Adapters for 12, 20 and 35 cc VAC
Male-Male Adapter
Male Luer Plugs
Female Luer Plugs
Qty.
Part No.
48/pk
12/pk
12/pk
10/pk
100/pk
WAT011390
186005587
WAT054260
WAT048160
WAT024310
WAT044395
WAT044385
Accessories for Extraction Columns and Cartridges
Oasis 96-well Elution Plate

(Requires Manifold Spacer)
Spacer for Elution Plate

(Included with Manifold Kit)
Description
Description
Qty.
Part No.

Extraction column connector
Inline precolumn filter kit
Replacement filters
Reservoir, 30 cc (for Oasis Plus, Light, Vac and Classic cartridges)
Reservoir, 60 cc (for Oasis Plus, Light and Vac cartridges)
Adapter, male-male Luer (for Oasis Classic cartridges)
Adapter (to attach reservoir to 1, 3 and 6 cc Oasis Vac cartridges)
Adapter (to attach reservoir to 12, 20 and 35 cc
Oasis Vac cartridges)
2nd vial rack for manifold
1/pk
1/pk
1/pk
1/pk
5/pk
48/pk
12/pk
100/pk
12/pk
186000262
WAT046910
WAT082745
WAT084560
WAT005139
725000417
725000418
WAT011390
186005587
WAT024310
WAT054260
10/pk
WAT048160
Qty.
Part No.
25/box
50/box
50/box
50/pk
WAT058942
WAT058943
WAT058958
WAT058959
725000417
725000418
NEW Waters Positive

Pressure-96 Processor
186003522
186005234
T he Waters Positive Pressure-96 Processor offers stat of the art operation for
96-well and 1 cc flangeless cartridge formats. Each of the 96 holes in the
processor is restricted in order to maintain constant pressure, even if all the
plate well positions are not filled. Positive pressure processing offers many
advantages over traditional methods, including:
SPE Vacuum Pump

(Includes two gauges
and pressure regulator)
Vacuum Box Gasket Kit
Manifold for Extraction Cartridges

Description
Highly uniform flow

from well to well
Superior flow for

viscous samples
Highly reproducible
assays
Easy-to-use design
Part No.
Waters extraction manifold, 20-position without rack

(includes 20 needle tips, 25 plugs, and ejector tool)
Waters extraction manifold, 20-position
(complete with rack for 13 x 75 mm tubes)
WAT200677
WAT200606
WAT200607
WAT200608
WAT200609
Description
Waters Extraction
Manifold
38
Waters Positive Pressure-96 Processor

96-flangeless cartridge holder
96-place sealing gasket
Elution positive pressure spacer
Gas supply adapter, includes 1/8 in to 1/4 in NPT
fitting, 6 feet of 1/4 in tubing
10 mL x 24 waste collection plate
Qty.
Part No.
1
1
1
1
186005521
186005523
186005522
405006528
186005524
186005586
www.waters.com
SaMPLE PREPaRaTION
Sep-Pak Sample Extraction Products
T he most referenced and widely used sample preparation technology
Sep-Pak bonded silica devices are recognized throughout the world and
remain the most referenced solid phase extraction product for sample
preparation. A diverse selection of formats and sorbents make Sep-Pak SPE
products ideally suited for all types of samples for GC, HPLC, and UPLC
analysis methods.
Formats:
Cartridges
96-well plate
Elution plate
Chemistries:
Reversed-phase (silica-based)
tC2bonded phase with low hydrophobic characteristics
Accell Plus QMAhydrophilic strong anion-exchanger

with large pore-size
C8bonded phase with moderate hydrophobicity
Accell Plus CMhydrophilic weak cation-exchanger

with large pore-size
C18monofunctional bonded phase, a Waters original

tC18tri-functional bonded phase with increased hydrolytic stability
Reversed or Normal-phase (less polar alternatives to silica)

Amino Propyl (NH2)basic polar bonded phase
Cyano Propyl (CN)polar bonded phase
Diolneutral polar bonded phase
PSAPrimary-Secondary Amine
Normal-phase
Silicapolar surface used to adsorb analytes from non-polar solvents
Alumina (A, B & N) a highly active grade of alumina that is available
in acidic, basic and neutral surface chemistries
Florisilpolar, highly active, weakly basic sorbent for adsorption of
low to moderate polarity species from nonaqueous solutions
Ion-exchange (silica-based)
Specialty
PoraPak RDXfor analysis of explosives in
ground and surface water, EPA-8330
Sep-Pak Dryanhydrous Na2SO4 for removal
of residual water from non-aqueous extracts
DNPH-Silicafor air analysis of aldehydes and
ketones, EPA-TO-11A, ASTM D-5791
X PoSurefor indoor air monitoring of aldehydes and ketones
AC2activated carbon used to concentrate pesticides and herbicides
PS2styrene-divinyl benzene polymer used to concentrate
pesticides and herbicides
Carbon Black / Amino Propylfor pesticides from food
Carbon Black / PSAfor concentrating pesticides from food
www.waters.com
39
SaMPLE PREPaRaTION
Sep-Pak Sorbent Selection Guide
Reversed Phase
C18
Si(CH3)2C18H37
tC18
SiC18H37
C8
Si(CH3)2C8H17
tC2
SiC2H5
Description
Applications
Chemistry
Silica-based bonded phase with strong

hydrophobicity; used to adsorb analytes
of even weak hydrophobicity from
aqueous solutions.

in serum, plasma or urine
Desalting of peptides
Trace organics in environmental
water samples
Organic acids in beverages
Similar in behavior to
reversed-phase HPLC columns
1.
2.
3.
4.
Particle Size: 55-105 m

Pore Size: 125
Surface Area: 325 m2/g
Carbon Load: 12%
Silica-based bonded phase with strong

hydrophobicity; trifunctional bonding
chemistry gives it an increased hydrolytic
stability over C18.
Similar to C18
1.
2.
3.
4.

Pore Size: 125
Carbon Load: 17%
Silica-based bonded phase with moderate

hydrophobicity; use for methods requiring
less retention than C18.

in serum plasma or urine
Peptides in serum
Plasma
1.
2.
3.
4.

Pore Size: 125
Carbon Load: 9%
Silica-based bonded phase with low

hydrophobic character; use for methods
requiring less retention than C8.
Similar to C18 and C8
1.
2.
3.
4.

Pore Size: 125
Carbon Load: 2.7%
Description
Applications
Chemistry
Silica-based polar bonded phase with basic

character; can be used as a polar sorbent,
like silica, with different selectivity for
acidic/basic analytes or as weak anion
exchanger in aqueous medium.
Phenols and phenolic pigments

Petroleum fractionation
Saccharides
Drugs and metabolites
1.
2.
3.
4.

Pore Size: 125
Carbon Load: 3.5%
Silica-based polar bonded phase; can be used

as less polar alternative to silica in normalphase applications or as less hydrophobic
alternative to C18 or C8 in reversed-phase.
Drugs
Drug metabolites
Pesticides
1.
2.
3.
4.

Pore Size: 125
Carbon Load: 6.5%
Silica-based polar bonded phase with neutral

character; can be used as an alternative to silica
in normal phase applications, where the acidic
character of silica is undesirable or as very weakly
interacting phase in aqueous applications.
Antibiotics from cosmetics

Isolation of proteins or peptides by
hydrophobic interaction chromatography
1.
2.
3.
4.

Pore Size: 125
Carbon Load: 9%
Silica-based bonded phase containing primary

and secondary amines. Similar selectivity to
aminopropyl but with higher pKas and increased
ion-exchange capacity.
Strong affinity for fatty acids, polar

pigments, and sugars
Potential for adsorption by chelation
4. pH range: 2-9
1. Particle sizes:
37-55 m
5. IEX capacity:
1.75 meg/g
2. Pore size: 60
3. Surface area: 450 m2/g
Reversed or Normal Phase
Aminopropyl
Si(CH2)3NH2
Cyanopropyl
Si(CH3)(CH2)3(CN)
Diol
Si(CH2)3OCH2CH(OH)CH2OH
PSA
SiC2H4NHC2H4NH2
40
www.waters.com
SAMPLE PREPARATION
Normal Phase
Silica
SiO2
Alumina
(A, B & N)
Al2O3
Florisil
MgOSiO2
Description
Applications
Chemistry
Polar sorbent, used primarily to adsorb analytes

from non polar solvents like hydrocarbons,
chloro- or fluoro-substituted hydrocarbons or
less polar esters and ethers; elution with more
polar solvents like polar esters, ethers, alcohols,
acetonitrile or water; the binding mechanism can be
hydrogen bonding or dipole-dipole interaction;
silica can also be used in aqueous medium as a
cation exchanger of intermediate strength.
General normal phase
1.
2.
3.
4.

Pore Size: 125
Activity Grade: high
Similar in use to silica; available in acidic, basic

and neutral high activity grades; alumina also
exhibits specific interactions with the -electrons of
aromatic hydrocarbons. More stable under high pH
conditions than silica.
Crude oil fractionation

Acidic and basic grades can also be
used as low capacity ion-exchangers
1.
2.
3.


Pore Size: 120
Activity Grade: high
Al N pH: 7.5
Al A pH: 4.5
Al B pH: 10.0
Polar, highly active, weakly basic sorbent for

adsorption of low to moderate polarity species from
non-aqueous solutions.
Specifically designed for the adsorption

of pesticides using official AOAC and
EPA methods
Other polychlorinated biphenyls (PCBs)
in transformer oil
1. Particle Size: 50-200 m

2. Pore Size: 60
3. Activity Grade: high
Description
Applications
Chemistry
Silica-based, hydrophilic, strong anion-exchanger

with large pore size. Used for extraction of anionic
analytes in aqueous and non-aqueous solutions.
Isolation of anionic proteins, e.g.,

immunoglobulins, enzymes
Acidic pigments from wines, fruit juices
and food extracts,
isolation of phenolic compounds
Peptide pool fractionation
1. Particle Size:
37-55 m
2. Pore Size: 300
3. Carbon: 6%
4. Counter Ion: Cl
5. pH Range 2-9
6. Loading Capacity:
200 mg
BSA/gram sorbent
7. Small Molecule
loading:
1.8-2.8 meq/gram
8. Ligand Density:
220 moles/g
Silica-based hydrophilic weak cation-exchanger with

large pore size; extraction of cationic analytes in
aqueous and non-aqueous solutions
Isolation of cationic proteins

Pesticides
Herbicides
Steroids
1. Particle Size:
37-55 m
2. Pore Size: 300
3. Carbon: 5.5%
4. Counter Ion: Na
5. pH Range 2-9
6. Loading Capacity:
175 mg Cytochrome
C/gram sorbent
7. Small Molecule
loading:
3.1-4.2 meq/gram
8. Ligand Density:
350 moles/g
Ion Exchange
Accell Plus QMA

Strong Anion-Exchanger
C(O)NH(CH2)3N(CH3)3+ Cl-
Accell Plus CM
Weak Cation-Exchanger
CO -Na+
2
Literature
References
A Sample Preparation Primer
and Guide to Solid-Phase
Extraction Methods Development,
www.waters.com
96-well Collection Plate Options for the

Waters Extraction Plate Manifold,
Waters 96 and 384-Well

Collection Plate specifications,
Waters Sep-Pak Sample

Extraction Products Brochure,
Sorbent Selection Guide

for SPE Wall Chart,
41
SaMPLE PREPaRaTION
Sep-Pak Cartridge Selection Guide
Plus
Short
Sorbent
C18
tC18
C8
tC2
Silica
Florisil
Accell Plus CM
Accell Plus QMA
Diol
Vac 1 cc/
100 mg
Vac RC/
100 mg
50/box
50/box
50/box
100/box
100/box
50/box
Part No./
Mass/Volume*
Part No./
Mass/Volume*
Part No./
Volume*
Part No./
Volume*
Part No./
Volume*
WAT020515
360 mg/0.7 mL
WAT036810
400 mg/0.8 mL
WAT036775
400 mg/0.8 mL
WAT052720
400 mg/0.8 mL
WAT023635
820 mg/1.6 mL
WAT036800
900 mg/1.4 mL
WAT023501
130 mg/0.3 mL
WAT036805
145 mg/0.4 mL
WAT036770
145 mg/0.4 mL
WAT052725
145 mg/0.4 mL
WAT023537
120 mg/0.4 mL
WAT023543
145 mg/0.3 mL
WAT023531
130 mg/0.4 mL
WAT023525
130 mg/0.4 mL
WAT023549
280 mg/0.35 mL
WAT023555
280 mg/0.35 mL
WAT023561
280 mg/0.35 mL
WAT023513
130 mg/0.3 mL
186004578
130 mg/0.3 mL
WAT023507
130 mg/0.3 mL
WAT023519
130 mg/0.4 mL
WAT051910
360 mg/0.85 mL
WAT054955
0.13 mL
WAT054960
0.11 mL
WAT054965
0.11 mL
WAT023590
0.2 mL
WAT036820
0.25 mL
WAT036785
0.25 mL
WAT052710
0.25 mL
WAT023595
0.25 mL
WAT023600
0.2 mL
WAT023625
0.25 mL
WAT023620
0.25 mL
WAT023575
0.1 mL
WAT023580
0.1 mL
WAT023585
0.1 mL
WAT023610
0.2 mL
186004561
0.2 mL
WAT023615
0.2 mL
WAT023605
0.25 mL
WAT036935
0.2 mL
WAT043410
0.25 mL
WAT043415
0.25 mL
WAT020520
690 mg/1.6 mL
WAT020525
910 mg/1.4 mL
WAT020550
360 mg/0.8 mL
WAT020545
360 mg/0.8 mL
Cyano Propyl (CN)
Vac 1 cc/
50 mg
Part No./
Mass/Volume*
Alumina B
PSA
Classic
Short/Long
50/box
Amino Propyl (NH2)
Plus
Light
Part No./
Mass/Volume*
Alumina A
Alumina N
Plus
Long
WAT020500
1710 mg/1.2 mL
WAT020505
1710 mg/1.2 mL
WAT020510
1710 mg/1.2 mL
WAT020535
360 mg/0.7 mL
186004538
360 mg/0.7 mL
WAT020540
360 mg/0.7 mL
WAT020530
360 mg/0.8 mL
WAT051900
690 mg/2.0 mL
WAT051960
900 mg/1.7 mL
WAT010910
360 mg/1.1 mL
WAT010835
360 mg/1.1 mL
WAT051800
1850 mg/1.8 mL
WAT051820
1850 mg/1.8 mL
WAT051810
1850 mg/1.8 mL
WAT010830
360 mg/0.85 mL
186004560
360 mg/0.85 mL
WAT010823
360 mg/0.85 mL
WAT054980
0.15 mL
WAT054985
0.12 mL
186004562
0.1 mL
WAT054975
0.13 mL
WAT036940
0.25 mL
WAT043460
0.25 mL
WAT043475
0.2 mL
186004567
0.2 mL
WAT043480
0.25 mL
Sep-Pak 96-Well Plates

Description
Part No.
Sep-Pak tC18 25 mg Plate

Sep-Pak Accell Plus QMA 100 mg Plate
Sep-Pak C18 40 mg Plate
42
186002319
186002320
186002321
186001917
186003966
www.waters.com
SAMPLE PREPARATION
Vac 3 cc/
200 mg
Vac 3 cc/
500 mg
Vac RC/
500 mg
Vac 6 cc/
500 mg
Vac 6 cc/
1g
Vac 12 cc/
2g
Vac 20 cc/
5g
Vac 35 cc/
10 g
50/box
50/box
50/box
30/box
30/box
20/box
20/box
20/box
Part No./
Volume*
Part No./
Volume*
Part No./
Volume*
Part No./
Volume*
Part No./
Volume*
Part No./
Volume*
Part No./
Volume*
Part No./
Volume*
WAT054945
0.42 mL
WAT054925
0.34 mL
WAT054940
0.34 mL
WAT020805
0.8 mL
WAT036815
1.0 mL
WAT036780
1.0 mL
WAT052715
1.0 mL
WAT020810
1.2 mL
WAT020815
0.8 mL
WAT020855
1.1 mL
WAT020850
1.1 mL
WAT020820
0.4 mL
WAT020825
0.4 mL
WAT020830
0.4 mL
WAT020840
0.8 mL
186004536
0.8 mL
WAT020835
0.8 mL
WAT020845
1.0 mL
WAT036945
0.8 mL
WAT043425
1.0 mL
WAT043430
1.0 mL
WAT043395
1.2 mL
WAT036790
1.1 mL
WAT054525
1.1 mL
WAT036905
2.0 mL
WAT036795
1.9 mL
WAT054570
1.9 mL
WAT052705
1.9 mL
WAT036910
1.9 mL
WAT043390
2.0 mL
WAT054590
1.9 mL
WAT054595
1.9 mL
WAT054580
0.8 mL
WAT054585
0.8 mL
WAT054575
0.8 mL
WAT054605
2.0 mL
186004537
2.0 mL
WAT054600
2.0 mL
WAT054610
1.9 mL
WAT036915
3.6 mL
WAT043380
3.5 mL
WAT054615
3.5 mL
WAT036925
8.0 mL
WAT043365
7.8 mL
WAT054660
7.8 mL
WAT043345
16.8 mL
WAT043350
16.3 mL
WAT054700
16.3 mL
WAT054930
0.53 mL
WAT054935
0.42 mL
WAT036950
1.2 mL
WAT043435
0.8 mL
WAT054505
1.1 mL
WAT054500
1.1 mL
WAT043400
1.2 mL
WAT043405
1.2 mL
WAT054545
1.2 mL
WAT054550
1.2 mL
WAT054535
0.5 mL
WAT054540
0.5 mL
WAT054530
0.5 mL
WAT054560
1.2 mL
186004563
1.2 mL
WAT054555
1.2 mL
WAT054565
1.1 mL
WAT043485
0.4 mL
WAT054515
0.8 mL
186004568
0.8 mL
WAT054520
1.0 mL
WAT036920
3.9 mL
WAT043385
3.6 mL
WAT054635
3.5 mL
WAT054640
3.5 mL
WAT054620
1.8 mL
WAT054625
1.8 mL
WAT054630
1.8 mL
WAT054650
3.6 mL
186004564
3.6 mL
WAT054645
3.6 mL
WAT054655
3.5 mL
WAT036930
11.0 mL
WAT043370
8.0 mL
WAT054675
7.8 mL
WAT054680
7.8 mL
WAT054670
3.9 mL
WAT054665
3.9 mL
WAT043375
3.9 mL
WAT054695
8.0 mL
186004565
8.0 mL
WAT054685
8.0 mL
WAT054690
7.8 mL
WAT043355
23.4 mL
WAT043360
16.8 mL
WAT054720
16.3 mL
WAT054725
16.3 mL
WAT054710
8.2 mL
WAT054715
8.2 mL
WAT054705
8.2 mL
WAT054740
16.8 mL
186004566
16.8 mL
WAT054730
16.8 mL
WAT054735
16.3 mL
*Hold up volume
Sep-Pak 96-Well Elution Plate

Description
Sep-Pak tC18 Elution Plate
www.waters.com
Part No.
186002318
43
SAMPLE PREPARATION
Sep-Pak Specialty Chemistries
Air Testing
Description
Part Number
Mass/Volume/Type
Chemistry
DNPH and XPoSure contains acidified dinitrophenylhydrazine

reagent coated on a silica sorbent. Used for the collection of
air samples and subsequent quantitation of aldehydes and
ketones by reaction to form the hydrazone derivative, and
analysis by HPLC. DNPH-Silica is specified in several EPA
procedures for the analysis of carbonyl compounds in air.
WAT037500
20/box
350 mg/0.7 mL/Plus Short
1. Particle Size: 55-105m
DNPH and XPoSure contains acidified dinitrophenylhydrazine

reagent coated on a silica sorbent. Used for the collection of
air samples and subsequent quantitation of aldehydes and
ketones by reaction to form the hydrazone derivative, and
analysis by HPLC. DNPH-Silica is specified in several EPA
procedures for the analysis of carbonyl compounds in air.
WAT039550
20/box
XPoSure
Larger particle dinitrophenylhydrazine coated

silica for use with personal air monitors.
WAT047205
20/box
Ozone Scrubber
For removing ozone interference with the analysis

of carbonyl compounds. Use in series with
Sep-Pak DNPH or XPoSure cartridge.
WAT054420
20/box
1.4 g/1.6 mL/Plus Short
1. 1.4 g of potassium iodide
Description
Part Number
Mass/Volume/Type
Chemistry
For the analysis of explosives in surface and ground water.

Meets or exceeds requirement of EPA Method 8330.
Reduces use of organic solvent by 10-fold. PoraPak RDX
is a divinylbenzene/vinylpyrrolidone copolymer.
WAT047220
30/box
500 mg/1 mL/6cc Vac
For removal of residual water from the SPE extract.
WAT054265
50/box
2.85 g/1.6 mL/Plus Long
1. 2.85 g anhydrous
sodium sulfate
Low ash content activated carbon, packed in a Sep-Pak Plus

short body cartridge. Common applications include the concentrate of pesticides and herbicides in water or food samples.
Sep-Pak AC2 is used in Japan Pesticide Method #17.
JJAN20229
50/box
1. Particle Size: 85 m
Styrene divinylbenzene copolymer packed in a Sep-Pak

Plus short body cartridge. PS2 applications include concentration of pesticides and herbicides in water. This cartridge is
used in Japan pesticide methods #5, 6, and 9. AC2 connected
in series with PS2 was adopted as the official method for
the extraction of pesticides from water by JPMHW.
JJAN20131
50/box
500 mg of carbon black/amino propyl bonded silica

separated by a frit. Typical application includes pesticide clean-up from food matrixes. Particularly useful for
removing coloring from samples before GC analysis.
186003369
30/box
500 mg carbon black,

500 mg Amino Propyl
/1.4 mL/6 cc Vac
1. 500 mg Carbon black

top layer, 37-105 m
500 mg carbon black,

500 mg PSA
/1.4 mL/6 cc Vac

[carbon-black, top layer]
150 mg/0.4 mL/Plus Light
DNPH Short
DNPH Long
Potassium/Iodine
2. Pore Size: 125

3. Coating: 14 moles/g or
5 moles/cartridge
800 mg/1.6 mL/Plus Long

2. Pore Size: 125
3. Coating: 14 moles/g or
10 moles/cartridge
2. Capacity: 4.2 mmoles

of ozone/cartridge
Food, Environmental and Biological Testing
Porapak RDX
DVB Vinylpryrrolidone
Sep-Pak Dry
Sodium Sulfate
AC2
Fine Activated
Carbon
PS2
Styrene - DVB
Carbon Black/
Amino Propyl
Carbon Black/
PSA
Two-layer sorbent bed used for pesticide clean-up in food

matrices prior to GC analysis. PSA provides alternative
selectivity compared to aminopropyl.
Primary-Secondary
186004590
30/box
2. Pore Size: 200
2. 400 mg/cartridge
activated carbon
1. Particle Size: 80 m
2. 300 mg cartridge
2. 500 mg Amino propyl, 55-105 m
2. 37-55 m [PSA, bottom layer]
Amine Silica
Accell Plus
QMA Carbonate
Silica-based, hydrophilic, strong anion-exchanger with a

carbonate counter-ion. Used for extraction of anionic analytes
in aqueous and non-aqueous solutions when the standard
chloride counter-ion will interfere with the analysis.
186004051
50/box
2. Pore Size: 300

3. Counter-ion: Carbonate
4. pH Range: 2-9
5. Ligand Density: 220 moles/g
44
www.waters.com
SaMPLE PREPaRaTION
Sep-Pak DNPH-Silica Cartridges

for Analyzing Formaldehyde, Other Aldehydes and Ketones in Air
Formaldehyde and other aldehydes are receiving increasing attention both
as toxic substances and as promoters in the photochemical formation of
ozone in air. Sources of aldehydes in residential buildings include plywood
and particle board, insulation, combustion appliances, tobacco smoke, and
various consumer products. Aldehydes are released into the atmosphere in
the exhaust of motor vehicles and other equipment in which hydrocarbon
fuels are incompletely burned.
T he most sensitive and specific method for analyzing aldehydes and
ketones is based on their reaction with 2,4-dinitrophenylhydrazine (DNPH)
and subsequent analysis of the hydrazone derivatives by HPLC. T he
hydrazones may be detected by absorbance in the ultraviolet region, with
maximum sensitivity obtained between 350 and 380 nm.
Airborne aldehydes have traditionally been collected by drawing a sample
through an impinger containing a solution of DNPH. However, the impinger
collector is generally cumbersome to use and is not well suited for high
flow rates or extended collection times due to solvent evaporation.
T he new Sep-Pak DNPH-silica cartridges meet the requirements of EPA
Method TO-11A and provide a convenient device for sample collection.
Using a vacuum pump, an air sample is drawn through the new Sep-Pak
DNPH-silica cartridge. T he aldehydes and ketones react with the DNPH and
form the hydrazone derivative, which is retained on the cartridge. Later, the
hydrazones are eluted from the cartridge with acetonitrile and analyzed by
HPLC. Detection limits can be as low as 3 ppbv for a 100 liter sample.
Advantages of Waters Sep-Pak DNPH-Silica Cartridges
T hese cartridges provide you with significant advantages when compared
to other techniques, such as liquid impingers, for the analysis of aldehydes
and ketones. In addition, a new high speed, high resolution HPLC application
has been developed to provide excellent quantitation
capability in the low parts-per-billion range.
Sep-Pak DNPH-silica cartridges meet the requirements of

EPA Method TO-11A and ASTM-D-5791-1
Results from impingers and these cartridges are in excellent agreement
Solvent consumption, solvent exposure and hazardous waste

disposal costs are reduced
Sep-Pak DNPH-silica cartridges provide superior convenience and

reproducibility, making them ideal for field sampling and process
monitoring applications
Sep-Pak DNPH-silica cartridges can save time and increase productivity
Increased safety.
Sep-Pak DNPH-Silica Cartridge

Description
Sep-Pak DNPH-silica cartridge
Sep-Pak DNPH-silica long body cartridge
www.waters.com
Qty
Part No.
20/box
20/box
WAT037500
WAT039550
HPLC Separation of DNPH Derivatives of

Common Aldehydes and Ketones
HPLC Conditions
Column:
Nova-Pak C18 3.9 x 150 mm
Sample:
Mixture of DNPH and DNPH derivatives
in acetonitrile.
Mobile Phase:
A: Water/Acetonitrile/Tetrahydrofuran
60/30/10 v/v/v
B: Water/Acetonitrile 40/60 v/v 3 mL
Gradient:
100% A for 1 minute, then linear gradient from
100% A to 100% B in 10 minutes
Flow Rate:
1.5 mL/min
Injection:
20 L
Detection:
360 nm
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
DNPH
Formaldehyde-DNPH
Acetaldehyde-DNPH
Acetone-DNPH
Acrolein-DNPH
Propionaldehyde-DNPH
Crotonaldehyde-DNPH
Butanone-DNPH
Butyraldehyde-DNPH
Benzaldehyde-DNPH
Isovaleraldehyde-DNPH
Valeraldehyde-DNPH
o-Tolualdehyde-DNPH
m-Tolualdehyde-DNPH
p-Tolualdehyde-DNPH
Hexaldehyde-DNPH
2,5-Dimethylbenzaldehyde-DNPH
Low-Level: Aldehyde Profile from Laboratory Air

Lab Air Sample
1. DNPH
2. Formaldehyde-DNPH
3. Acetaldehyde-DNPH
4. Acetone-DNPH
5. Butanone-DNPH
6. Isovaleraldehyde-DNPH
Cartridge Blank (calculated for 100 liter sample)

1. DNPH
2. Formaldehyde-DNPH
0.35 ppbv
3. Acetaldehyde-DNPH
0.27 ppbv
4. Acetone-DNPH
0.34 ppbv
4.8 ppbv
1.2 ppbv
118.0 ppbv
0.8 ppbv
0.7 ppbv
The sample was obtained in a chemical research laboratory using a portable sampling pump.
One hundred liters of air was drawn through the cartridge at 0.65 L/min. The sample was found to
contain low concentration of formaldehyde (4.8 ppbv), acetaldehyde (1.2 ppbv) and methyl ethyl ketone
(0.8 ppbv), but a significant amount of acetone (118 ppbv). Significant concentrations of acetone are
frequently found in laboratories because of its widespread use as a solvent.
High-Level: Aldehyde Profile from Diluted Auto Exhaust Emissions

Sample (8.4 L at 1 L/min) collected at US EPA
Atmospheric Research Assessment Lab. Courtesy of
Dr. S. B. Tejada.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
DNPH
Formaldehyde-DNPH
Acetaldehyde-DNPH
Acetone-DNPH
Acrolein-DNPH
Propionaldehyde-DNPH
Crotonaldehyde-DNPH
2-Butanone-DNPH
Butyraldehyde-DNPH
Benzaldehyde-DNPH
o-Tolualdehyde-DNPH
m-p-Tolualdehyde-DNPH
2,5-Dimethylbenzaldehyde-DNPH
2290 ppbv
367 ppbv
26 ppbv
8 ppbv
12 ppbv
36 ppbv
24 ppbv
15 ppbv
32 ppbv
5 ppbv
11 ppbv
3 ppbv
45
SaMPLE PREPaRaTION
Ozone Scrubber Cartridges

Ozone has been s hown to interfere wit h t he
analysis of carbonyl compounds in air samples
that have been drawn through cartridges containing silica-coated with 2,4-dinitrophenylhydrazine
(DNPH). Waters Ozone Scrubber cartridges are
designed to remove this ozone interference.
T hese disposable devices are intended for use in series combination with
the Waters Sep-Pak DNPH-Silica cartridges or X PoSure Aldehyde Sampler
cartridges. One Ozone Scrubber cartridge replaces the 1/4 diameter by
36 long copper ozone denuder located in the heated zone of sampling
systems used for outdoor air monitoring (PAMS program).
Eac h Ozone Scrubber cartridge contains 1.4 g of granular potassium
iodide. W hen air containing ozone is drawn through this packed bed,
iodide is oxidized to iodine, consuming t he ozone. T he t heoretical
capacity of a single cartridge is 4.2 mmoles of ozone (200 mg). T he
particle size of t he potassium iodide granules is optimized for good
mass transfer and flow c haracteristics.
Flow Schematic for Air Sampling System
Air
Sample
In
O3 Scrubber
Cartridge
DNPH-Silica
or X PoSure
Cartridge
Vacuum
Sampling
Pump
Ozone Scrubber
Description
Ozone Scrubber
Qty.
Part No.
20/box
WAT054420
Waters X PoSure Aldehyde Sampler Cartridges for Monitoring Aldehydes in Indoor Air
Based on an extension of our DNPH coating
technology, XPoSure aldehyde sampler cartridges
are t he most sensitive active samplers
available today.
Low-Level Example: Aldehyde Profile from Laboratory Air

1
1.
2.
3.
4.
5.
6.
Highest Sensitivity
Compared to existing sampling tube tec hnology whic h have high and
variable backgrounds, X PoSure cartridges are guaranteed to give
consistent low aldehyde backgrounds, cartridge-to-cartridge, lot-to-lot.
High-Collection Efficiencies
You can ac hieve > 95% collection efficiencies for all aldehydes at flows
of up to a liter per minute. And, you only need to use one cartridge
no breakthrough bed is necessary.
Low Pressure DropUse with Portable Personal Sampling Pumps
Large particle size and higher porosity frits make the X PoSure cartridge
compatible with personal sampling pumps.
DNPH
Formaldehyde-DNPH
Acetalhyde-DNPH
Acetone-DNPH
Butanone-DNPH
Isovaleraldehyde-DNPH
6
Lab Air Sample
Cartridge Blank
0
10
12
14
Min.
The above sample was collected in a chemical research laboratory using a portable sampling pump. One hundred
liters of air was drawn through the cartridge at 0.65 L/min. The chromatogram shows ormaldehyde (4.8 ppbv),
acetaldehyde (1.2 ppbv), acetone (118 ppbv), butanone (0.8 ppbv), and isovaleraldehyde (0.7 ppbv).
Easy-to-Use
Sample, elute and shoot, its that easy. Youll never have to break open and
manipulate a glass tube again. And because the cartridges are made from
high density polyethylene (HDPE), breakage is not a concern.
T he figure on t he right s hows two traces. An actual cartridge blank
demonstrating extremely low background levels and as an actual
laboratory air sample.
46
X PoSure Aldehyde Sampler Cartridge

Description
XPoSure Aldehyde Sampler Cartridge
Qty.
Part No.
20/box
WAT047205
www.waters.com
SaMPLE PREPaRaTION
PoraPak Rdx Sep-Pak Extraction Cartridge

for the Analysis of Explosives in Surface and Ground Waters
Activate, Load, Elute, and Shoot

Flow 15 mL acetonitrile through
cartridge at 10 mL/min
Designed to meet or exceed the QA/QC requirements of EPA method 8330,

it is ideal for environmental testing laboratories supporting Department of
Defense remediation programs.
Flow 30 mL water through

cartridge at 10 mL/min
High Sensitivity
Load 500 mL water sample

at 10 mL/min
PoraPak Sep-Pak cartridges contain PoraPak Rdx resin, a specially prepared,

specially cleaned divinylbenzene/vinylpyrrolidone copolymer, packed in
a high purity polypropylene syringe barrel. With the lowest guaranteed
backgrounds and the highest cartridge-to-cartridge, lot-to-lot consistency,
the Waters PoraPak Rdx column is the most sensitive technology available
today and allows you to perform analysis at sub ppb levels.
Elute with 5 mL acetonitrile

Dilute to 40% acetonitrile
with HPLC grade water
Inject 100 L onto HPLC
Unmatched Recoveries
T he specially prepared resin is highly selective for nitroaromatic and
nitramine compounds, resulting in recoveries of 90% or greater. Recovery
data from preconcentrating 500 mL of explosives standards in sterile
water at two concentrations on PoraPak Rdx Sep-Pak Vac columns. Number
of replicates = 7.
1 ppb
% Recovery
% RSD
10 ppb
% Recovery
% RSD
HMX
TNB
RDX
100.5
95.9
90.9
6.7
3.5
6.4
100.5
99.3
98.7
3.9
3.3
3.2
DNB
TNT
TETRYL
99.5
97.0
89.0
3.2
3.0
6.4
99.2
102.
102.8
3.2
3.7
4.7
NB
3,5-DNA
2,4-DNT
96.5
91.2
97.3
2.5
3.3
3.4
97.9
98.2
99.9
2.8
3.6
3.4
2,6-DNT
2-Am-DNT
4-Am-DNT
94.5
92.4
90.0
3.4
5.2
4.9
98.7
98.0
97.2
3.4
3.7
4.1
4-NT
2-NT
89.5
96.8
4.3
6.6
100.4
93.4
3.7
3.0
Compound
Isocratic Separation of Method 8330 Analytes

HPLC Conditions
Column:
Mobile Phase:
Flow Rate:
Injection Volume:
Temperature:
Detection:
Sample:
Explosives
Blank
Min.
Achieve the lowest method detection limits using Waters PoraPak Rdx cartridges.
The chromatogram above shows the analysis of reagent H2O spiked at the 1 ppb level.
The bottom chromatogram is a reagent (H2O) blank.
PoraPak Rdx Cartridges and Accessories

Description
Increase Productivity and Reduce waste

By using Pora Pak Rdx cartridges, you can reduce the amount of organic
solvent used per sample by 10-fold and decrease your sample prep
time by 3X.
Waters Nova-Pak C8, 3.9 x 150 mm

82:18 water/isopropanol
1.0 mL/min
100 mL
24 C
UV @ 254 nm
1 ppb explosives concentrated
40-fold on PoraPak Rdx, in 60:40
water/acetonitrile
PoraPak Rdx cartridges

Tubing, Tefzel, 1/8 inch o.d. x 0.040-inch i.d.
Sep-Pak Vac adapter
60 cc Sep-Pak reservoir
Male-male adapter
Qty.
Part No.
30/box
WAT047220
WAT023344
WAT054260
186005587
WAT024310
12/box
12/box
Sep-Pak Dry SPE Cartridge

Waters Sep-Pak Dry cartridges are packed with 2.85 g of anhydrous
sodium sulfate. T hese cartridges are designed to remove residual water
from the SPE extract.
Sep-Pak Dry Cartridge

Description
Sep-Pak Dry Cartridge
www.waters.com
Qty.
Part No.
50/box
WAT054265
47
SAMPLE PREPARATION
Certified Sep-Pak Solid-Phase Extraction Cartridges
Manufacturing
Our world-class manufacturing facilities strive to improve quality
expectations for SPE product performance. We manufacture under the
highest quality standard in the industry including ISO 9001:2000,
ISO 13485:2003 and current Good Manufacturing Practices (CGMP). Each
Certified Sep-Pak Product is thoroughly QC tested using:
Sorbent Specifications based on:
Contaminants including hydrocarbons and other
environmental contaminants
Sorbent functionality including:
ligand density
particle size distribution
surface activity
As a pioneering leader in solid-phase extraction, Waters is has advanced SPE

performance and quality by offering Certified Sep-Pak sample preparation
products. By manufacturing these devices to strict performance and cleanliness specifications, we ensure that the detection limits and performance of
your analytical methods will not be compromised by interfering substances
commonly found in SPE hardware.
Chromatographic performance
Assembly Specifications based on:
Frits and barrels dimensional tolerance
Chromatographic testing of total residual extractables including:
Hydrocarbons
Plasticizers
Anti-oxidants
Improve Workflow and Reduce Solvent Waste

Certified Sep-Pak sample preparation devices are available in the most
commonly used formats and sorbents to allow easy integration into your
sample preparation protocol. Reduced background interferences reduce
solvent waste by eliminating unnecessary solvent pre-washing steps that
are often required for trace residue methods.
Sorbent bed voiding

Consistent sample flow c haracteristics
Comparison of Extracted Interference Levels in Organochlorine Pesticide Analysis at 1 ppm

Standard
SPE Procedure
Scan EI+
TIC
2.00e8
Certified Sep-Pak Florisil, 3 cc/500 mg
TCP
1
2 3
5
9 10
15,16
13
12
11
14
Part Number: 186004611
17
Contaminants found in
2
UT BHT
BHA
competitors SPE devices:

BHB
UFA
BHA. butylated hydroxyanisole
Competitor Cartridge
Scan EI+
TIC
2.00e8
UT. unknown terpene

BHT. butylated hydroxytoluene
UFA. unknown fatty alcohol
UFE
UFE
UFE
UFE. unknown fatty ester
aldehyde
U. unknown
H. hydrocarbon (alkane)
Certified Sep-Pak Florisil
Scan EI+
TIC
2.00e8
GC Analysis
Instruments:
3
8
10
11
1. -HCH (hexachlorocyclohexane)
2. -HCH
3. -HCH (lindane)
4. -HCH
5. heptachlor
12
13
6 aldrin
7. heptachlor epoxide
8. endosulfan I
9. DDE
10. dieldrin
14
15
16
17
11. endrin
12. endosulfan II
13. DDD
14. endrin aldehyde
15. endosulfan sulfate
18
19
Elute:
5 mL 90:10 hexane/acetone (v/v)
BHB. butylatedhydroxybenz-
Load:
1 mL hexane extract sample
using gravity flow
20
16. DDT
17. methoxychlor
21 min
Column:
Injection:
Temperature Program:
Evaporate:
To just under 1 mL and adjust
to 1.0 mL final volume
Waters Quattro micro GC with Agilent 6890 Full Scan

50-400 m/z
RTX 5 MS Column 30 M x 0.25 mm (0.25 m df)
2.0 L (splitless) 250 C injection, 300 C transfer to MS
Initial Temperature: 50 C for 1 minute
Ramp: 10 C/minute
Final Temperature: 320 C
TCP (trichlorophenol) is added to the target compound mix as a polar probe to test sorbent specificity. Quantitative removal
of TCP is expected using Florisil sorbent under these SPE conditions.
1 REF: EPA Method 8080A, Rev. 1, September 1994.
48
www.waters.com
SaMPLE PREPaRaTION
Certified Sep-Pak Sorbent Selection Guide
C18
Silica
Alumina
(A, B, N)
Silica-based, trifunctionally-bonded octadecyl sorbent
High carbon load provides excellent hydrolytic

stability for a wide range of samples
Strong hydrophobic sorbent used to adsorb analytes

of even weak hydrophobicity from aqueous solutions
Typical applications include drugs and their

metabolites in serum, plasma or urine, desalting
of peptides, trace organics in environmental water
samples, organic acids in beverages.
Unbonded, highly-activated silica stationary phase
A polar sorbent for analyte isolation from nonpolar solvents like hydrocarbons and less
polar esters and ethers
Analyte retention can occur through hydrogen bonding

or dipole-dipole interactions in non-aqueous samples
Silica provides a slightly acidic surface for moderate

cation-exchange interactions in aqueous samples
Elution with more polar solvents like polar esters,

ethers, alcohols, acetonitrile or water.
Alumina very similar to silica; however, the alumina

surface tends to be slightly more stable under high
pH conditions than unfunctionalized silica
T he aluminum oxide surface provides an extremely

polar surface for analyte retention and has properties
of a Lewis acid
Depending on the sorbents surface treatment,
alumina is available is three forms Alumina A,
Alumina B, and Alumina N
Alumina exhibits specific interactions with the

-electrons of aromatic hydrocarbons, making it
useful for applications like crude oil fractionation
Acidic and basic grades can be used as low-capacity

ion-exchangers.
Florisil
Very-polar, highly-active, weakly-basic sorbent for

adsorption of low to moderate polarity species from
non-aqueous solutions
Specifically designed for the adsorption of pesticides

using official AOAC, EPA, and JPMHLW regulated
methods
Applications include polychlorinated biphenyls

(PCBs) in transformer oil.
Ordering Information
Description
Part No.
C18
Certified Sep-Pak Vac, 3 cc, 200 mg, 50/box
Certified Sep-Pak Vac, 6 cc, 1 g, 30/box
186004618
186004619
186004620
186004621
Silica
186004614
186004615
186004616
186004617
Alumina A
186004602
186004603
186004604
Alumina B
186004605
186004606
186004607
Alumina N
186004608
186004609
186004610
Florisil
186004611
186004612
186004613
Literature
References
www.waters.com
Environmental Analysis Solutions,

Food Testing Solutions,

Environmental Chromatography
Methods Guide,
Food Safety
Application Notebook,
Certified Sep-Pak Solid-Phase

Extraction Cartridges Brochure,
Certified Sep-Pak Florisil for Sample Cleanup
Organochlorine Pesticides and PCBs,
49
SAMPLE PREPARATION
Waters Extraction Manifold for Use With Solid-Phase Extraction Cartridges
T he vacuum manifold has the capacity to process up to twenty samples
simultaneously. T he extraction manifold has enhanced features designed
for use with conventional silica-based, solid-phase extraction cartridges
as well as modifications that allow you take full advantage of the unique
performance characteristics of our Oasis HLB extraction cartridges.
Just look at what this manifold has to offer:
A Precision-machined Delrin cover with alignment posts
for quick and easy alignment with test tube rack.
B Vacuum gauge placement on cover, not in fluid path allows
for quick and easy waste removal at bottom by vacuum.
C
Description
Part No.
20 position extraction manifold without rack

20 position extraction manifold with 13 x 75 mm test tube rack
WAT200677
WAT200606
WAT200607
WAT200608
WAT200609
Accessories and Spare Parts for the Waters Extraction Manifolds

Description
Enhanced vacuum control valve designed for use with

Waters Oasis HLB extraction cartridges, allows for a quick
and momentary rise in vacuum above the frit bubble point
at the touch of a finger.
D High purity polypropylene needle valves and needle tips with

minimum dead volume (Opening and closing the valves is
required to prevent silica-based SPE cartridges from drying out).
Extraction Manifolds (Includes 20 needle tips, 25 plugs, and ejector tool)
Qty.
Part No.
Needle valves, 20/pk (required when using silica-based SPE

cartridges) (not required for use with Oasis Extraction Cartridges)
Needle tips, 20/pk
Cover, 20 position without gauge assembly
Gauge assembly, vacuum
Reservoir, glass with outlet valve
Outlet valve kit
Gasket for cover
Ejector tool
Luer Plugs, 25/pk
Rubber ball ring (for vacuum gauge assembly)
Reversible Vial Rack for 1 mL or 4 mL autosampler vials
2 mL vial rack for manifold
13 x 75 mm test tube rack
Reservoir, 30 cc (for Sep-Pak Plus, Light,
48/pk
Vac, and Classic cartridges)
Reservoir, 60 cc (for Sep-Pak Plus, Light, and Vac cartridges)
12/pk
Adapter, male-male Luer (for Sep-Pak Classic cartridges)
100/pk
Adapter (to attach reservoir to 1, 3, and
12/pk
6 cc Sep-Pak Vac cartridges)
Adapter (to attach reservoir to 12, 20,
10/pk
and 35 cc Sep-Pak Vac cartridges)
Vacuum pump (110 V, 60 Hz)
Vacuum pump (220 V, 50 Hz)
WAT200685
WAT200691
WAT200686
WAT200687
WAT200688
WAT200689
WAT200690
WAT058839
WAT058851
WAT058840
WAT058871
186005234
WAT200678
WAT200679
WAT200680
WAT200681
WAT011390
186005587
WAT024310
WAT054260
WAT048160
WAT085114
WAT085115
SPE Vacuum Pump

(Includes a single gauge. Pressure can be
controlled using relief valve on manifold.)
50
www.waters.com
SAMPLE PREPARATION
Sep-Pak Cartridge Accessories

Description
30 cc Reservoir
60 cc Reservoir
Reservoir Adapters for 1, 3, and 6 cc VAC
Reservoir Adapters for 12, 20, and 35 cc VAC
Male-Male Adapter
Male Luer Plugs
Female Luer Plugs
Sep-Pak Cartridge Connections Kit

Qty.
Part No.
48/pk
12/pk
12/pk
10/pk
100/pk
WAT011390
186005234
WAT054260
WAT048160
WAT024310
WAT044395
WAT044385
Contains a selection of the most commonly needed fittings, adapters,

valves, and tubing for use with Sep-Pak cartridges.
Description
Part No.
Sep-Pak Connections Kit
WAT011400
NEW Waters Positive Pressure-96 Processor

T he Waters Positive Pressure-96 Processor offers stat of the art operation for
96-well and 1 cc flangeless cartridge formats. Each of the 96 holes in the
processor is restricted in order to maintain constant pressure, even if all the
plate well positions are not filled. Positive pressure processing offers many
advantages over traditional methods, including:
Description
96-flangeless cartridge holder
96-place sealing gasket
Elution positive pressure spacer
Gas supply adapter, includes 1/8 in to 1/4 in NPT
fitting, 6 feet of 1/4 in tubing
10 mL x 24 waste collection plate
www.waters.com
Qty.
Part No.
1
1
1
1
186005521
186005523
186005522
405006528
186005524
186005586
Highly uniform flow from well to well
Superior flow for viscous samples
Highly reproducible assays
Easy-to-use design
Literature
References
Waters Positive Pressure-96
Processor Brochure,
51
SAMPLE PREPARATION
Environmental and Food Solution Kits
ACQUITY PFC Column Kit
Carbamate Analysis Kit for Food Testing
Optimized for trace level detection of Perfluorinated Compounds (PFCs)

wit h t he ACQUIT Y system, t his kit contains t he ACQUIT Y BEH C 18,
1.7 m, 2.1 x 50 mm column, the ACQUIT Y PFC Isolator Column and
PFC reference standards.
Waters Carbamate Analysis Kit for Food Testing includes a Waters Carbamate
column, Oasis HLB cartridges, vials and reference standards. Optimized for
use with AOAC methods for Carbamate Analysis, these products simplify
your analysis while increasing your confidence in the results.
Description
Part No.
ACQUITY PFC Column Kit
176001692
Description
Carbamate Analysis Kit for Food Testing
Part No.
186004719
ACQUITY PFC Analysis Kit
EPA Method 1694 Analysis Kit
Waters ACQUIT Y PFC Analysis Kit includes Oasis SPE cartridges, PFC
calibration and reference standards, Certified vials, ACQUITY columns and
the necessary instrument components to optimize your instrument for trace
level detection of PFCs.
Waters EPA Method 1694 Analysis Kit includes the XTerra MS C 18,
Atlantis HILIC columns, and Oasis HLB cartridges; all of whic h are
specified in the EPA Method.
Description
EPA Method 1694 Analysis Kit
Description
Part No.
ACQUITY PFC Analysis Kit
176001744
Carbamate Analysis Kit for Environmental Testing

Waters Carbamate Analysis Kit includes a Waters Carbamate column, Oasis
HLB cartridges, vials, and reference standards. Optimized for use with EPA
Method 531.2, these products simplify your analysis while increasing your
confidence in the results.
Description
Part No.
Carbamate Analysis Kit for Environmental Testing
52
Application Specific Kits
176001740
Part No.
176001634
ACQUITY Bisphenol A Column & Method Kits

T he ACQUIT Y UPLC Bisphenol A Column and Method Kits are fully
compliant with ASTM Method D7574-09. Waters ACQUIT Y solution
provides optimum resolution and sensitivity for the analysis of Bisphenol
A in water. T he Column Kit includes the ACQUITY BEH C18 column and
ACQUITY Isolator column. T he Method Kit also includes Oasis HLB SPE
cartridges and LCMS Certified sample vials.
Description
ACQUITY Bisphenol A Column Kit
ACQUITY Bisphenol A Method Kit
Part No.
176001955
186004932
www.waters.com
SaMPLE PREPaRaTION
NEW Ostro Sample Preparation
T he Faster Way to Cleaner
Ostro sample preparation products provide a novel solution for t he
cleanup of phospholipids in plasma and serum. Requiring minimal to
no method development and using a simple protocol, this tec hnology
can be quickly implemented in order to optimize your laboratorys
workflow. Providing cleaner, more reproducible extracts than competitive phospholipid removal devices or tec hniques, t he Ostro 96-well
plate allows for more sensitive analyses, increased sample throughput,
and reduced instrument downtime.
Removes significantly more phospholipids for cleaner extracts.
Improves reproducibility for more consistent, robust methods.
Increases throughput with easy-to-implement protocol.
Protocol
Minimizing method development time, the standard Ostro protocol will provide
excellent results for a wide variety of acidic, basic and neutral compounds.
Phospholipid Removal
Place Ostro onto

collection plate.
350000
Sum of Area Counts of 5 Phospholipids
300000
Pipette 50-200 L of
plasma into wells.
Sum of 5 abundant phosphatidylcholine

containing phospholipids with molecular
weights 496, 524, 704, 758, and 806.
250000
Forcefully add 1% formic acid in acetonitrile,

3:1 solvent:plasma. (Methanol not recommended).
200000
150000
Mix thoroughly by aspirating

3X with pipette.
100000
Filter samples using 15 vacuum manifold for 5-10 min.

or 60 psi positive processor manifold for 5 min.
50000
0
Ostro
LLE
SSLE
Analyze samples by direct injection, dilution &

injection, or by evaporation, reconstitution & injection.
Reproducibility
Comparative % removal of total phospholipids from 6 different lots
of plasma using Ostro (0.19% RSD), phospholipid removal plate from
competitor A (24.5% RSD) and phospholipid removal plate from
competitor B (40.9% RSD).
* For sample volumes 50 L or less, a higher solvent to plasma ratio may be neccessary.

Description
Qty.
Part No.
186005518
100
Ostro Sample Preparation 96-Well Plate (25 mg)
90
% Phospholipid Removal
80
70
60
50
40
Literature
References
30
20
10
0
Ostro
www.waters.com
Competitor A
Competitor B
Ostro Sample Preparation Products Brochure,

Ostro Technical Note: Crosstalk,

Ostro Technical Note: Recovery,

53
SaMPLE PREPaRaTION
DisQuE Dispersive Sample Preparation Solutions
Dispersive sample preparation, commonly referred to as QuEChERS, is a
simple and straightforward sample preparation technique suitable for multiresidue pesticide analysis in a wide variety of food and agricultural products.
Waters DisQuE Dispersive Sample Preparation Kit contains conveniently
packaged centrifuge tubes with pre-weighted sorbents and buffers designed
for use with regulatory methods.
Waters DisQuE dispersive sample preparation is a well-proven, high throughput
sample preparation method that offers several advantages over traditional
SPE techniques:
Easy and straightforward method that requires little training
Decreases the time spent on sample preparation
Consistent and reliable devices from a single vendor eliminates

the need for independent sourcing and improves quality
Recovery Data for T hree Types of Sample Matrices Fortified at 10 ng/g
Efficient, cost effective method of sample preparation for

a wide variety of commodities
125
Baby Food
Mango
Avocado
Eliminates outsourcing
Improves consistency in extraction product
Improves lab efficiency and workflow
Recovery %
100
80
60
40
20
0
Chromatogram Showing 402 Pesticide

Residues at 10 ppb ng/g In One 10 Minute Run
Pesticide
UPLC Conditions
LC System:
Column:
Column Temp:
Sample Temp:
Flow Rate:
Mobile Phase A:
Mobile Phase B:
Gradient:
Total Run Time:

Injection Volume:
MS Conditions
MS System:
Ionization Mode:
Capillary Voltage:
Desolvation Gas:
Cone Gas:
Source Temp:
Acquisition:
Collision Gas:
Waters ACQUITY UPLC System

ACQUITY UPLC BEH C18
2.1 x 100 mm, 1.7 m
40 C
4 C
0.450 mL/min
98:2 water: methanol + 0.1% formic acid
Methanol + 0.1% formic acid
Time
A%
0.00
90
0.25
90
7.75
0
8.50
0
8.51
90
10 min
20 L, full loop injection
Waters ACQUITY TQ Detector

ESI positive polarity
1 kV
Nitrogen, 800 L/Hr, 400 C
Nitrogen, 5 L/Hr
120 C
Multiple Reaction Monitoring (MRM)
Argon at 3.5 mBar
DisQuE Kitted Solutions

Complete solutions and kitted methods add value to your laboratory
function by addressing the need for simple, easy-to-follow protocols that
require very little training.
Waters offers two versions of the DisQuE Dispersive Sample Preparation
Kit that conform to established methods. Each kit contains convenientlypackaged, pre-filled tubes for 100 samples.
AOAC Method 2007.01
DisQuE Extraction (Tube 1): A 50 mL centrifuge tube containing 1.5 g
anhydrous sodium acetate and 6 g of anhydrous magnesium sulphate.
DisQuE Clean-Up (Tube 2): A 2 mL centrifuge tube containing 150 mg
anhydrous magnesium sulphate and 50 mg of primary secondary amine
(PSA) sorbent.
CEN Method 15662
0.5
1.5
2.5
3.5
4.5
5.5
6.5
7.5
8 Min.
DisQuE Extraction (Tube 1): A 50 mL centrifuge tube containing 4 g

MgSO4, 1 g NaCl, 1 g trisodium citrate dehydrate, 0.5 g disodium
hydrogencitrate sesquihydrate.
DisQuE Clean-Up (Tube 2): A 2 mL centrifuge tube containing 150 mg
MgSO4, 25 mg PSA, and 25 mg C18.
54
DisQuE Dispersive Sample Preparation Kit
www.waters.com
SaMPLE PREPaRaTION
DisQuE Extraction and Clean-up Tubes

DisQuE extraction and clean-up tubes are available separately for customized applications and greater flexibility. T he clean-up tubes are available
in the standard 2 mL size as well as a 15 mL size for sample enrichment.
Waters offers PSA and C18 sorbent blends to help remove the matrix from
your sample.
- C18 removes very non-polar interferences, such as fats
- PSA removes polar interferences, including sugars and organic acids
In some instances, when the sample contains excess fat or high amounts of
c hlorophyll, it is beneficial to add graphitized carbon black (GCB) or
additional C18 sorbent. T he additional sorbents are available in bulk and can
be provided for this purpose.
DisQuE Dispersive Sample Preparation Products
Description
Part No.
DisQuE Kits
Extraction Tubes
TUBE 1: 50 mL Tube contianing:

DisQuE AOAC Dispersive 1.5g Sodium Acetate and 6 g MgSO4
TUBE 2: 2 mL Tube contianing: 150mg
SPE Kit
MgSO4 and 50 mg PSA
TUBE 1: 50 mL Tube containing: 1 g
Trisodium Citrate Dihydrate, 0.5 g Disodium
DisQuE CEN
Hydrogencitrate Sesquihydrate, 1 g NaCl and
Dispersive SPE Kit
4 g MgSO4
TUBE 2: 2 mL Tube containing: 150 mg
MgSO4, 25 mg PSA, 25 mg C18
176001676
176001903
Individual Extraction Tubes (TUBE 1)
50 mL tube containing 1.5 g

Sodium Acetate and 6 g MgSO4
(blue cap)
50 mL tube containing 1 g
Trisodium Citrate Dihydrate,
0.5 g Disodium Hydrogencitrate
Sesquihydrate, 1g NaCl, and
4 g MgSO4 (white cap)
DisQuE 50mL Tube/AOAC DisQuE 50 mL Tube contianing: 1.5 g Sodium

- Acetate
Acetate and 6 g MgSO4
DisQuE 50 mL Tube containing: 1 g
DisQuE 50mL Tube/
Trisodium Citrate Dihydrate, 0.5 g Disodium
CEN - Citrate
Hydrogencitrate Sesquihydrate, 1 g NaCl and
4 g MgSO4
186004571
186004837
Individual Clean-up Tubes (TUBE 2)
Clean-Up Tubes
2 mL tube
2 mL tube
2 mL tube
containing
containing
containing
150 mg MgSO4 150 mg MgSO4, 150 mg MgSO4
and 50 mg PSA
50 mg PSA,
and 25 mg PSA
(white cap)
and 50 mg C18
(yellow cap)
(blue cap)
2 mL tube
containing
150 mg MgSO4,
25 mg PSA
and 25 mg C18
(green cap)
DisQuE 2mL Tube

- AOAC
DisQuE 2mL Tube
- AOAC with C18
DisQuE 2mL Tube
- CEN
DisQuE 2mL Tube
- CEN with C18
DisQuE 15mL Tube/
MgSO4/PSA
DisQuE 15mL Tube/
MgSO4/PSA/C18
DisQuE 2 mL Tube contianing: 150 mg

MgSO4 and 50 mg PSA
DisQuE 2 mL Tube containing: 150 mg
MgSO4, 50 mg PSA, and 50 mg C18
MgSO4 and 25 mg PSA
DisQuE 2 mL Tube contianing: 150 mg
MgSO4 and 150 mg PSA
186004572
186004830
186004831
186004832
186004833
186004834
Bulk Sorbents
Graphitized Carbon Black. 25 g Bottle
C18, 100g Bottle
186004835
WAT035672
*PSA: Primary-Secondary Amine silica bonded sorbent
Literature
References
15 mL tube containing
900 mg MgSO4 and
150 mg PSA (white cap)
www.waters.com
15 mL tube containing
900 mg MgSO4, 150 mg PSA,
and 150 mg C18 (blue cap)
A Rapid Method for the

Screening and Confirmation
of over 400 Pesticide
Residues in Food,
Literature Reference
720002628EN
Determination and
Confirmation of Priority
Pesticide Residues
in Baby Food,
720002812EN
DisQuE Dispersive Sample Preparation Kit
DisQuE Dispersive Sample

Preparation Brochure,
720003048EN
55
SaMPLE PREPaRaTION
PoraPak Rxn Cartridges
for Post Synthesis Cleanup
PoraPak
TM
Post Synthesis Cleanup
Waters PoraPak Rxn, a family of polymer-based chromatography products

for superior cleanup of synthetic reactions. Pora Pak Rxn products are
available in two chemistries:
PoraPak Rxn CX, a strong cation-exchange sorbent
PoraPak Rxn RP, a reversed-phase sorbent
PoraPak Rxn sorbents are available in fritted syringe-barrel devices in 6,

20, and 60 cc volumes. T he resins are also sold in bulk units, and custom
configurations are available on request.
New Solutions for Faster Results
PoraPak Rxn sorbents are based on copolymers that exhibit the
following properties:
Hard material that does not develop increasing

back pressure with flow
Little swelling or shrinking across a range of

solvents and pH extremes
Low hydraulic resistance enables flow by gravity
pH extreme tolerance without dissolution or hydrolysis,

both limitations of silica-based sorbents
T his combination of physical and chemical properties makes PoraPak Rxn

cartridges ideal for synthesis cleanup. T he polymers characteristics and
particle size maintain gravity, pressure -or vacuum-assisted flow; even
when reaction mixtures contain precipitate that may contribute additional
resistance to flow. T he sample will still pass through the cartridge.
56
PoraPak Post-Synthesis Cleanup Products
T he polymer used in PoraPak Rxn products is resistant to shrinking or swelling in the organic solvents typically used in synthetic reactions. Tests
with the following solvents demonstrate that the packed bed maintains
good flow properties:
DCE
DMF
DCM
THF
DMSO
Acetone
Some medicinal chemists are familiar with silica-based chromatographic

products for reaction cleanup. One of the limitations of these silica-based
ion-exchange materials is pH. Silica will dissolve at high pH, while bonded
phases are hydrolyzed at low pH; both conditions result in loss of sample
and/or impurities (silica and bonded phase) collected in product fractions.
PoraPak Rxn polymer-based chromatographic phases are stable at extreme
pH. T his feature permits using pH as a very powerful tool to create a
separation, particularly in ion-exchange mode.
Providing Separations Solutions
Waters is highly respected worldwide for its expertise in chromatography.
Coupled with our ability to seamlessly link critical instrumentation,
c hemistries, separation tec hnologies, and software, this expertise puts
us in a unique position to deliver value-added solutions to our customers.
Manufacturing
Our world-class manufacturing facilities are continuously expanded
and upgraded to keep pace with market demand for our new and existing products. We manufacture under the highest quality standards in the
industry, including ISO9001:2000, ISO 13485:2003 and Current Good
Manufacturing Practices (cGMP).
www.waters.com
SaMPLE PREPaRaTION
Condition
Load
Wash
Elute
Cleanup Challenges
Challenge
Solution
Convert or remove high boiling solvent before

next reaction step - product is a base.
Load reaction mix on PoraPak Rxn CX cartridge and pass through high
boiling point solvent such as DMSO.
Wash with methanol.
Elute using 5% ammoniated methanol. Achieve sample enrichment and save time
by evaporative removal of a low boiling point solvent before the next step.
Separate product (bases) from reactants

and reaction solvent.
Load on PoraPak Rxn CX cartridge using a catch-and-elute strategy.

Wash with methanol and elute with ammoniated methanol.
Remove TFA used in the reaction from bases.
Load reaction mixture on PoraPak Rxn CX cartridge using a catch-and-elute strategy.

TFA passes through unretained.
Convert from a solvent used in the last synthesis

before Prep LC to get sample in the starting mobile
phase conditions.
Load on PoraPak Rxn CX cartridge using a catch-and-elute strategy.

Evaporate the fraction and reconstitute the product in the Prep LC starting solvent.
Remove water and TFA from fractions collected

from Prep LC for faster dry down.
Load on PoraPak Rxn CX cartridges using a catch-and-elute strategy.

www.waters.com
57
SaMPLE PREPaRaTION
Choose the Best Product and Technique for Your Cleanup

Pora Pak Rxn products are available in two sorbents: CX, a strong CationeXc hange resin and RP, a Reversed-Phase resin. T he illustrations below
recommend the sorbent to use and the separation tec hnique to apply
during cleanup. T he c hoice of the appropriate sorbent and tec hnique
depends upon the nature of the reaction products, reactants, solvents,
and/or preparative columns.
Reaction Products
Reaction Components
Pre-and PostPreparative Column
Technique
Recommended Product
Bases
Catch and Elute
PoraPak Rxn CX
Acids
Pass T hrough
PoraPak Rxn CX
Neutrals
Adsorption
PoraPak Rxn RP
TFA removal
Catch and Elute
PoraPak Rxn CX
Convert from high boiling

point solvent for bases
Catch and Elute
PoraPak Rxn CX
Fractionate sample
before Prep for bases
Catch and Elute
PoraPak Rxn CX
Remove water from post

column fraction for bases
Catch and Elute
PoraPak Rxn CX
Catch and Elute
Adsorption
In the catch and elute technique, the reaction mixture is loaded onto
the cartridge; the analytes of interest are retained by the sorbent. A wash
step follows to remove additional reaction components from the cartridge.
A strong solvent is used to elute the analytes from the cartridge. Sample
concentration results when the final elution volume is smaller than the
load volume.
In the adsorption-chromatography technique on a reversed-phase sorbent,

the neutral compounds are moving down the chromatographic column in
the presence of organic solvent, albeit slowly, for small-percent organic
washes. Load the reaction mixture in high water content (75% water for
example), wash to remove polar compounds, and then increase the organic
content in large increments to elute neutrals. T he increase is determined
by the hydrophobicity and MW of the compounds.
Pass T hrough
In the pass-through technique, the sorbent does not retain the compounds
of interest, but does retain some class(es) of compounds and impurities,
that can be discarded with the column. Fractionation is achieved by the
interaction between the impurities in the reaction mixture and the sorbent.
No sample enrichment occurs during the solid-phase extraction (SPE) step.
58
www.waters.com
SaMPLE PREPaRaTION
Cleanup Procedures
Using PoraPak Rxn CX: Strong Cation-Ion Exchange Catch-and-Elute and Pass-T hrough Protocols
Catch and Elute
Pass T hrough
Condition Step
100% methanol
Condition with an organic solvent such as methanol.

T his step is important to wet the sorbent.
Condition Step
100% methanol
Load Step
Fraction collected
contains acids,
neutrals and
reaction solvent
Pour the reaction mixture into the cartridge.

Let the reaction mixture flow by gravity.
Load Step
Fraction collected
contains acids,
neutrals and
reaction solvent
Wash Step
100% methanol
fraction contains
more impurities,
acids and neutrals
Elution Step
5% ammoniated
methanolfraction
contains bases
Pass-T hrough Strategy the analytes of interest are unretained

Catch-and-Elute Strategy
analytes are retained by ion-exchange interaction.
Wash with 100% methanol.

Pass-T hrough Strategy the analytes of interest are unretained
and are eluted in the wash. Combine the fraction collected
in the load step with this wash fraction.
Wash Step
100% methanol
fraction contains
acids and neutrals
Catch-and-Elute Strategy the wash step contains

unwanted reaction components.
Catch-and-Elute Strategy
elute bases using 5% ammoniated methanol.
Pass-T hrough Strategy the retained compounds are unwanted
reaction components; no need for further elution step.
Recombine Load
and Wash Steps
For PoraPak Rxn RP: Reversed-Phase Protocol

Adsorption
For a compound to be retained, a
reaction, carried out in a polar organic
solvent will have to be concentrated by
evaporation and/or diluted with water
(3-to-1 or greater). T his dilution brings
the organic content down to 25% or less
so as to enable reversed-phase retention.
T he generic protocol, shown to the right,
can be used as a starting point to clean
up reaction mixture.
Condition the sorbent with polar organic solvent, preferably

the polar organic solvent used in the reaction. Follow with
an equilibration step using organic/water solvent,
containing no less than 75% water.
Condition Step
Generic protocol Condition with methanol followed by water.
Pour the reaction mixture into the cartridge.

Let the reaction mixture flow by gravity.
Load Step
Generic protocol Load water:organic solvent

(at least 3:1). Fraction collected contains polar compounds.
Designed to only remove the undesired reaction

components, not the analytes.
Wash Step
Generic protocol
Wash 1 Water. Fraction contains polar compounds.
Wash 2 Water:methanol (1:1). Fraction collected contains
less polar compounds.
Wash with a stronger solvent to elute the compound(s) of interest.
Elution Step
Generic protocol Methanol. Fraction collected

contains hydrophobic compounds.
www.waters.com
59
SaMPLE PREPaRaTION
Cleanup of a Reductive Amination Mixture

Reaction Mixture Before Cleanup
Reductive amination is a common reaction carried out in medicinal

c hemistry laboratories. In this example, a Pora Pak Rxn CX cartridge is
used to fractionate the reaction mixture.
Crude reaction mixture. MBA, the starting amine,

is present in small concentration with the product
and an excess of the aldehyde.
3.0
2.8
2.6
2.4
2.2
2.0
1-Naphthaldehyde
C
R
Sodium triacetoxyborohydride in DCE
H
+
R
R'
H2O
4-Methoxybenzylamine (MBA)
R'
+H2
1.8
PRODUCT
(>80% yield)
1.6
N
R
R'
H C
R
1-Naphthaldehyde
1.4
1.2
1.0
8.0e-1
N-(Naphthylmethyl)methoxybenzylamine (product)
MBA
6.0e-1
4.0e-1
2.0e-1
0.0
UPLC Conditions
Instrument:
Column:
Column Part Number:
Mobile Phase A:
Mobile Phase B:
Gradient and Flow Rate:
Injection Volume:
Column Temperature:
Detector:
UV Wavelength:
Load Step
Catch-and-Elute
Procedure
ACQUITY UPLC System

ACQUITY UPLC BEH Shield RP18, 2.1 X 100 mm, 1.7 m
186004049
0.01% formic acid
100% acetonitrile
Condition Step
100% methanol
Collected fraction from the loading step. The fraction that

passes through the cartridge contains aldehyde which is not
retained on the cation exchanger.
1.5
71% NA in Load Eluate
1.0
3% Product in Load Eluate

Time
Flow Rate
A(%)
B(%)
(min)
(mL/min)
Initial
0.42
95
5
7
0.42
1
99
2 L
30 C
ACQUITY UPLC PDA
250-340 nm (total absorbance mode)
Curve
5.0e-1
Load Step
Initial
6
0.0
Wash Step
Wash Step
100% methanol
When the cartridge is washed with methanol, additional

aldehyde elutes in this fraction.
2.5
2.0
1.5
10% Product in Wash Eluate
1.0
Elution Step
5% ammoniated
methanol
11% NA in Wash Eluate
5.0e-1
0.0
Elution Step
The cartridge is washed with ammoniated methanol, eluting
all the bases. This fraction contains the product.
2.5
2.0
Using the catch-and-elute procedure to clean up this synthesis mixture enables a quick collection of
a limited number of fractions, increasing the certainty of finding the product rapidly. Assay the product
fraction for confirmation, then begin the evaporation of the more volatile elution solvent.
78% Product
in Eluate
1.5
1.0
5.0e-1
0.0
0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0 Min
Removal of TFA from Reversed Phase Prep-LC Fractions; Isolation of the Purified Free-Base Product
Removal of T FA from Prep-LC
product fraction is important
as residual T FA has been linked
to compound degradation. T his
simple protocol removes T FA
and water from product fraction
for rapid evaporation. After
evaporation the compound is in
free-base form.
Product (NMBA)
TFA
2 .5 2
Elute
2 .6 1
Wash 2
60
2:MRM of 1 Channel ESTIC

1.23e6
Elute

3.74e6

1.23e6
Wash 2

3.74e6
Wash 1
LC-MS(MS) chromatograms
showing product only in the
elute fraction and all TFA in
the load and wash fractions

3.74e6
2. 93
Wash 1

1.23e6
2. 92

3.74e6
Load
2.0
2.2
2.4
2.6
2.8
3.0 Min.

1.23e6
Load
1
5 Min.
www.waters.com
SAMPLE PREPARATION
Product Guidelines
T he following tables should be used as guidelines for loading capacity,
flow rates, and volumes of wash and elution solvents. T hese tables take into
consideration the dimension of the device and the bed volumes suggest
appropriate flow rates and volumes for the condition, wash and elute steps.
Maximum Capacity
Guidelines
Configuration
PoraPak Rxn RP
PoraPak Rxn CX
Retained Compounds
Retained Base
T he reaction mix can be poured into the cartridge and allowed to flow by
gravity. If the flow is too slow, simply add a small amount of pressure or
vacuum to increase it. If there is precipitate in the reaction vessel, rinse
the vessel with more loading solvent and add to the top of the cartridge.
During the washing steps, product trapped by the precipitate may be
dissolved and can be retained on the packed bed for later elution under the
right solvent conditions.
6 cc/150 mg
15 mg/50 mole
15 mg/50 mole
Wash and Elution Volumes (mL)
6 cc/400 mg
40 mg/150 mole
40 mg/150 mole
Device
20 cc/2 g
200 mg/350 mole
200 mg/350 mole
60 cc/5 g
500 mg/2000 mole
500 mg/2000 mole
T he capacity is for the total of all compounds in the reaction that will be
retained. For cation exchange, the capacity is for all bases in the reaction
mixture including product and reactants.
Consider smaller loads than maximum capacity guidelines or choose a
larger device to avoid compound break through.
Condition
Wash
Elute
6 cc/150 mg
3 mL
4 mL
4 mL
6 cc/400 mg
5 mL
10 mL
10 mL
20 cc/2 g
20 mL
20 mL
20 mL
60 cc/5 g
45 mL
45 mL
45 mL
T his table provides guidelines for volumes used in the condition, wash and
elution steps for the various sizes of PoraPak Rxn cartridges.
Flow Rate Guidelines (mL/min)

6 cc cartridge
2 mL/min
20 cc cartridge
5 mL/min
60 cc cartridge
9 mL/min
Flow rate guideline is meant to provide a flow for reaction loading step.
PoraPak Rxn Cartridges and Bulk Material

Description
6 cc Flanged cartridges, 400 mg, 30/pk
6 cc Flangeless cartridges, 400 mg, 30/pk
20 cc cartridges, 2 g, 20/pk
60 cc cartridges, 5 g, 10/pk
Bulk, 200 mL /container
www.waters.com
Part No.
PoraPak Rxn CX
Part No.
PoraPak Rxn RP
186004541
186004542
186004543
186004544
186004569
186004545
186004546
186004547
186004548
186004570
61
SaMPLE VIaLS aNd aCCESSORIES

Waters Autosampler Vials
Choosing the Right Vial and
Septum for Your Application
T here are three decisions you need to make when c hoosing the correct
vial for your application: the septum, the closure and the vial itself. Read
through the selection options below to determine the proper combination for
your application. For your convenience, Waters offers many of these choices
as combination packs. T he vial, cap, and septum come pre-packaged as
packs of 100 for ease and convenience in ordering.
Waters is a leading manufacturer of analytical instrumentation and

consumable products. We understand the importance of autosampler vials
for the performance of analytical instrumentation. T here are many factors
to consider in selecting the proper vial:
Septa Selection Guide

PTFE

Recommended for single injection applications
Ideal for use in MS applications
Needle design
Optic and robotic specifications
Excellent solvent resistance and chemical compatibility
Autosampler tray design
Volatility
Does not reseal upon puncturing
Not recommended for long-term sample storage
Chemical compatibility
Cleanliness
Sample volume
PTFE/Silicone
At Waters, we take all of these factors into consideration in the design,

manufacture and delivery of our vials and accessories. Unlike our
competition, who offer Type I, 33-expansion glass in North America
and Type I, 51-expansion glass in Europe or Japan, Waters single
source manufacturing produces Type I, 33-expansion glass, the lowest
free ion glass available, for worldwide distribution.
Waters Certified Vials Product Lines

Waters offers two lines of certified vials. Both lines are certified as being
within dimensional tolerances and are tested for chemical cleanliness by
instrumentation methods.
Waters LCGC Certified Vials

T he LCGC Certified Vials are tested by HPLC using UV detection. T he
HPLC test was developed to look for trace levels of chemicals used in the
manufacturing and packaging process. T hese chemicals include; lubricants,
surfactants, antistatic and antioxidants from packaging. T he tests are run
on each batch of vials, after they have been packaged for several days, to
ensure cleanliness. An additional headspace GC Test is done to look for
proper curing of the silicone septa.
Waters LCMS Certified Vials

LCMS certified vials are tested using MS detection. In this test, we took
an unbiased approach and look for any ionized masses regardless of the
source. T he test is run in scan mode with specifications set on total ion
count and presence of clusters in the high mass range.
62
Sample Vials and Accessories
Recommended for multiple injections and sample storage
Demonstrates excellent resealing characteristics
PTFE chemical resistance until punctured, then the septum

will have the chemical compatibility of silicone
Working temperature range from -40 C to 200 C
Pre-slit PTFE/Silicone

Provides adequate venting to prevent vacuum formation in

sample vial, delivering excellent sample-draw reproducibility
Eliminates coring from bottom draw-port needles
Good resealing capabilities
Recommended for multiple injections
Working temperature range from -40 C to 200 C
PE Septumless

Same advantages as PTFE
Literature
References
Sample Vials & Accessories Brochure,
Waters LC/MS Certified
Sample Vials W hitepaper,
Determination of the Level of Ion

Suppression from LC/MS Vials,
Waters Certified Sample Vials
Technical W hitepaper,
www.waters.com

Vial Closures Guide
Vial Selection Guide
Vials are available in three closure types: crimp, snap, and screw cap. Each
closure has its advantages and disadvantages.
Type 1, 33-Expansion Borosilicate Glass
Crimp caps squeeze the septum between the rim of the glass vial and the
crimped aluminum cap. T his forms an excellent seal preventing evaporation.
T he septum stays seated during piercing by the autosampler needle. T he
crimp cap vial requires crimping tools to carry out the sealing process. For
few samples, manual crimper tools are the best choice. For large numbers of
sample, automated crimpers are available.
Snap caps are an extension of the crimp cap system of sealing. A plastic
cap is stretc hed over the rim of the vial to form a seal by squeezing the
septum between the glass and the stretc hed plastic cap. T he plastic cap
creates tension when trying to return to its original size. T his tension forms
the seal between glass, cap and septum. Plastic snap caps do not require
any tools to assemble.
Snap caps are not as effective a seal as other closures.

If the fit of the cap is very tight, the cap is hard to

apply and may be subject to crack.
If the fit is too loose, the seal is poor and the
septum may dislodge.
Screw caps are universal. Screwing the cap applies a mechanical force that
squeezes the septum between the glass rim and the cap. Screw caps form
an excellent seal and mechanically hold the septum in place during piercing.
No tools are required for assembly.
LectraBond screw caps are available through Waters. T his screw cap
has a PT FE/silicone septum bonded to the polypropylene cap, using a
non-solvent bonding process. T his bonding tec hnology is designed to
keep the septum/cap together during shipment and assembly onto vials.
T he bond will aid in preventing dislodging of the septum during use,
but the primary sealing mec hanism is the mec hanical force applied by
tightening the cap to the vial.
Cap tightening is the mechanism that forms the seal and holds the septum
in place during needle insertion. T here is no need to over-tighten the cap,
as it can compromise the seal and lead to dislodging. T he septum starts to
cup or indent when you begin to over-tighten.
Cap Design
Strength Design
Comment
Crimp
Excellent seal
Requires tools
Snap
Moderate seal
Fast, no tools, some cap cracking
Screw
Excellent seal
Universal
T he most chemically-inert glass available, generally used in high precision

laboratories to prevent alteration of test results. It has an expansion
coefficient of approximately 33x107 C and is composed primarily
of silicon and oxygen, with trace amounts of boron and sodium.
Type 1, 51-Expansion Glass
More alkaline t han 33-expansion glass and is adequate for many
laboratory uses. It has an expansion coefficient of 51x107 C and is
composed primarily of silicon and oxygen, with trace amounts of boron.
All amber glassware is 51-expansion glass.
Deactivated Glass (DV )
For glass-sensitive compounds, glass vials are treated with gas phase
reactive organosilane to produce a hydrophobic glass surface. Vials treated
by this procedure can be stored indefinitely.
Polypropylene Plastic
Polypropylene is a non-reactive plastic and can be used where glass is
not an appropriate option. Polypropylene vials can be incinerated while
still sealed, minimizing exposure to potentially hazardous substances. T he
maximum temperature use is: 135 C.
Deactivated Glass Vials (DV ) and Inserts
Eliminates adsorbtion of compounds onto the
glass surface when working with biological or
pharmaceutical compounds, natural products,
pesticides and herbicides. T he surface modification is permanent, resulting in an indefinite
shelf life.
Waters Alliance Total Recovery Vial

Specifically designed for the side draw-port
needle and the factory needle draw depth
settings of the Waters Alliance 2690/2695
HPLC. T his vial delivers maximum sample
capacity (~1 mL) with minimum residual
volume (~9 L).
Waters Maximum Recovery Vial

Specifically designed for the bottom drawport needle of the Waters ACQUITY UPLC and
Alliance HT HPLC Systems. T his vial delivers
maximum sample capacity (~1.5 mL) with
minimum residual volume. T he 9 mm cap
makes it ideal for use with Agilent HPLC and
GC Systems.
www.waters.com
63

Screw Cap 12 x 32 mm Vials for ACQUITY UPLC Systems
T his selection of 12 x 32 mm vials are the most commonly ordered vials by customers using Waters ACQUITY UPLC Systems.
Clear
Amber
Max Recovery
Amber Max
300 L PP
750 L PP
Clear Glass
with Septumless Cap
600000668CV
600000669CV
600000670CV
600000755CV
186003886C
For PEEK and

Metal-Tipped Needles
LCMS Certified Combination Packs

Vial, Cap and Pre-slit Silicone/PTFE Septum
LCGC Certified Combination Packs
Bonded Pre-slit Silicone/PTFE Septum
186000307C
186000847C
186000327C
Bonded Pre-slit Silicone/PTFE Septum Deactivated
186000307DV
186000847DV
186000327DV
186004132C
186004133C
186004168C
Combination with PE Septumless Cap
186004132C
Combination Packs
186002639
186004112
186005221
Injectable Volumes ACQUITY UPLC

Max
1600 L
1600 L
1100 L
1100 L
210 L
530 L
1600 L
Residual*
165 L
165 L
22 L
22 L
20 L
70 L
165 L
All items come in quantities of 100 unless otherwise noted.
* If you need to access more sample, go to the advanced setting screen in the Sample Manager Instrument Method Editor to change the needle placement.
For the Amino Acid ACQUITY UPLC System and the MassTrack Amino Acid Analysis Solution System, use of Waters Total Recovery Vials are recommended, Part Number 186004631.
ACQUITY UPLC Vial Descriptions
ACQUITY UPLC Vial Holder

Description
Part No.
48-Well Vial Holder
405003743

1
Clear 12 x 32, Type 1, 33-Expansion Glass, Screw Neck with Quick Thread Design,
(6 mm Opening, 9 mm Cap).
Amber 12 x 32, Type 1, 51-Expansion Glass Screw Neck with Quick Thread Design,
Waters Maximum Recovery Vial, 12 x 32, Type 1, 33-Expansion Glass, Screw Neck
with Quick Thread Design (6 mm Opening, 9 mm Cap).
Waters Amber Maximum Recovery Vial, 12 x 32, Type 1, 51-Expansion Glass,

Screw Neck with Quick Thread Design (6 mm Opening, 9 mm Cap).
Polypropylene 12 x 32, 300 L Screw Neck with Quick Thread Design

(6 mm Opening, 9 mm Cap). Reformulate Clean PP Vial.

(6 mm Opening, 9 mm Septumless Cap).
Waters Vials Selector

T he Waters Vials Selector is designed to simplify the process of selecting
the best vial solutions for your system and application requirements.
T he selector offers vial options matc hing the criteria entered, suc h as
the system you are using, sample volume, detection method and light
sensitivity of analytes. As a registered user, you will be notified of all
future updates.
For more information about Waters Certified Vials please visit
T his interactive tool requires that the user has Adobe Flash Player 8.
64
www.waters.com
Settings for ACQUITY UPLC Vials

Vial Column Number*
Description
Total Height (mm)
Vial Depth (mm)
Residual Volume
Needle Placement
Comments
1,2,7
Screw Cap Vial
38.3
32.0
165 L
2 mm
Preset
1,2,7
150 L or 300 L Insert Inside Vial 1 or 2
38.3
31.0
4 L
3 mm
Advanced Settings
3,4
Max Recovery Vial
38.3
32.0
22 L
2 mm
Preset
300 PP Vial
38.3
31.0
20 L
3 mm
Advanced Settings
750 PP Vial
38.3
31.5
70 L
3 mm
Advanced Settings
*Please reference the listing of Vials Descriptions on pages 78 and 79 for more details.
The table above shows vial depths, residual volumes and needle placements
for several products. In the ACQUITY UPLC system, the default needle
placement for 48-well vial holders with a 2 mL vial (ANSI 48 vial 2 ml
holder) is automatically preset and brings the needle down to a depth
which is 2 mm from the bottom of the vial. T he residual sample volume
remaining in the vial for a 2 mm offset is recorded in the table above.
For situations where you need to access more of the sample, or if you need
to keep the needle further from the bottom of the vial, go to the advanced
setting screen in the Sample Manager Instrument Method Editor to change
the needle placement.
Please note that you must go to the advanced settings and change the
needle placement to 3 mm for vial number 5 in the table above. You will
also have to review needle settings when using glass inserts.
Waters ACQUITY UPLC System Sample Manager

T he Waters ACQUIT Y UPLC System Sample Manager incorporates
several tec hnology advancements. T he Sample Manager loop design
and injection modes maintains low dispersion and facilitates fast cycle
times. T he system performance is monitored and diagnosed through
the console software. It uses needle-in-needle sampling for improved
ruggedness and a needle calibration sensor for increased accuracy.
A variety of sample holder formats (vials or tubes) and microtiter
plate formats (deep well, mid height) can also be accommodated in a
thermostatically-controlled environment. Within the ACQUIT Y UPLC
Sample Manager Instrument Method Editor, a number of parameters can

be customized for your specific task, including depth, as shown here, to
confer maximum sample format flexibility.
For further information on setting vial depth offsets, see the ACQUIT Y
UPLC Operators Guide (information documentation set for ACQUIT Y
UPLC Part Number 716001664) or visit the ACQUIT Y UPLC Sample
Manager Instrument Method Editor On-Line Help.
Waters ACQUITY UPLC System Sample Manager Version 1.30 and later
Sample Needle
Vial Depth
Typical 2 mL Vial
2 mm Default Offset
www.waters.com
65

Plates for ACQUITY UPLC Systems
96-Well Plates
Plate
384-Well Plates
186002643
186002481
186002482
186002632
100
50
50
50
50
350 L
800 L
2 mL
250 L
100 L
Polypropylene Cap Mat 50/pk
186002483
186002483
186002484
Clear Polyester Heat Seal 100/pk
186002788
186002788
186002788
186002788
186002788
Aluminum Foil Laminate Heat Seal 100/pk
186002789
186002789
186002789
186002789
186002789
35 L
15 L
20 L
15 L
15 L
Part No.
Max Volume
Residual Volume
186001437 (DV)2
650 L
15 L
850 L
15 L
96-Well Plate with 700 L Glass Insert, 1/pk
186000349 (DV)2
650 L
15 L
96-Well Plate with 1 mL Glass Insert, 18/pk
186000855 (DV)2
850 L
15 L
Pack Size
Well Volume
186002631
Sealing Options
Residual Volume in ACQUITY at

default needle placement of 2 mm*
* If you need to access more sample, go to the advanced setting screen in the Sample Manager Instrument Method Editor to change the needle placement.
Glass Inserts for 96-Well Plates

Description
Plates for Quick-Load Glass Widest Opening for Inserts, 20/pk
700 L Glass Quick-Load, 1/pk
1 mL Glass Quick-Load, 1/pk
1
2
1860014381
186001436 (DV)
Sealing Cap for 700 L Glass Insert Square Well Seals Against the Well Wall PTFE/Silicone, 5/pk
186000857
Sealing Cap for 1 mL Glass Inserts Seals in the Glass PTFE/Silicone, 10/pk
186000856
Plate 186001438 must be used with the quick load units.

When (DV) appears beside the part number, a deactivated version of this product can be ordered by adding DV to the right of the part number.
Quick-Load Inserts for 96-Well Plates
Quick-load packs are the easy and fast way to load your plate with glass inserts
66
www.waters.com

Heat Seals
Heat Seal for ACQUITY UPLC Systems
Heat Sealer
Part No.
115 Volt
186002786
240 Volt
186002787
Heat Sealer Operating Guidelines

For more information regarding heat sealers, download the data sheet
number 720001330EN at http://www.waters.com/library
Heat Seal
Temperature Range
Solvent Range
Clear Polyester
From -80 C to 80 C
Good for Most Lab Solvents
Aluminum Foil Laminate
From -200 C to 90 C
Good for Most Lab Solvents
Recommendation
Best for Long Term Storage
Clear Polyester Heat Seal

T he clear polyester heat seal is a non-conductive seal. For applications requiring
DMSO, the plates should be stored at 4 C.
Position the seal with the shiny side facing up. Apply heat using the Waters heat sealer
for 2 to 3 seconds in both directions following the instructions found on page 9 in the
heater manual, part number: 720001330EN.
Heat sealer dimensions: 5.75 x 13 x 6

(140 x 330 x 150 mm)
Aluminum Foil Heat Seal

T he aluminum foil laminate heat seal is good for most solvents used in laboratories.
For applications requiring DMSO, the plates should be stored at 4 C.
Position the seal with the white side facing up. Apply heat using the Waters Heat
Sealer for 2 to 3 seconds in both directions following the instructions found on page 9
in the heater manual, part number: 720001330EN.
Both of these seals can be peeled off by hand. For plate storage, apply a new
unpierced seal or polypropylene cap mat.
Literature
References
Sample Vials and Accessories Brochure,
Waters Heat Sealer User Manual,
96-well Collection Plate Options for the
Waters Extraction Plate Manifold,
Waters 96 and 384-Well
Collection Plate Specifications,
ACQUITY TQD System featuring the TQ detector and sample organizer
www.waters.com
67

Most Commonly Used Vials for Alliance Systems
T his selection of 12 x 32 mm vials are the most commonly ordered vials by customers using Waters Alliance Separations Modules. T his page is intended
to be a quick selection guide. For the complete selection of vials and accessories for Alliance, turn to pages 70-71.
Clear
Amber
Max Recovery
300 L PP
10 mm Cap Clear
Total Recovery
Amber Max
Clear Glass with

Septumless Cap
10
11
12
13
14
15
600000668CV
600000669CV
600000670CV
600000671CV
600000755CV

WAT270946C
Bonded Silicone/PTFE Septum

186004132C
Combination Packs
186000307C
186000847C
186000327C
Combination Deactivated
186000307DV
186000847DV
186000327DV
186002639*
186000385C
186003886C
Max
1100 L
1100 L
280 L
1100 L
950 L
1100 L
Residual
750 L
750 L
20 L
750 L
9 L
750 L
Max
1700 L
1700 L
1500 L
290 L
1700 L
1500 L
1700 L
Residual
170 L
170 L
22 L
10 L
170 L
22 L
170 L
186000385DV
Injectable Volumes Alliance 2690/2695
Insert
WAT094171 (DV)1 WAT094171 (DV)1
150 L with Poly Spring

Max Volume Injection/Max Residual Volume
144 L/6 L
144 L/6 L
Alliance 2690/2695
Alliance 2790/2795
WAT094171 (DV)1
144 L/6 L
144 L/6 L
Compatible Systems
* Not certified
For more details, see vials descriptions on pages 78 and 79.
68
www.waters.com
SAMPLE VIALS AND ACCESSORIES

Plates for Alliance HT Systems
96-Well Plates
Plate
384-Well Plates
186002643
186002481
186002482
186002632
100
50
50
50
50
350 L
800 L
2 mL
250 L
100 L
186002483
186002483
186002484
186002788
186002788
186002788
186002788
186002788
186002789
186002789
186002789
186002789
186002789
35 L
15 L
20 L
Pack Size
Well Volume
186002631
Sealing Options
Alliance 2795 Residual Volume

Heat Sealer
Description
Description
Part No.
115 Volt
186002786
240 Volt
186002787
Roller for Cap Mats

Description
Part No.
Roller for Mats
Part No.
186002633
1860014381
186001437(DV)2
186001436(DV)2
186000349(DV)2
186000855(DV)2
Sealing Cap for 700 L Glass Square Well PTFE/Silicone, 5/pk
186000857
Sealing Cap for 1 mL Round Well Seals in the Glass PTFE/Silicone, 10/pk
186000856

2
Selection of plates and cap mats
Roller for cap mats
Heat sealer
Waters Plate Selector

T he Waters Plate Selector is designed to help to you c hoose the most
appropriate AQCUITY UPLC plate for your application. Simply enter basic
information on your sample volume and sample quantity and the selector
will identify the best plate, along with alternative options.
For more information about Waters Plate Selector, please visit:
www.waters.com/plateselector
www.waters.com
69

Screw Cap 12 x 32 mm Vials for Alliance Systems
Clear
Amber
Max Recovery
300 L PP
750 L PP
10 mm Cap Clear
Total Recovery
Amber Max
16
17
18
19
20
21
22
23
Vial, Cap and Silicone/PTFE Septum
600000751CV
600000752CV
600000749CV
600000750CV
600000754CV
600000668CV
600000669CV
600000670CV
600000671CV
600000755CV
186000272C
186000846C
186000326C
WAT270946C 2
186000384C
186003885C
186000272DV
186000846DV
186000326DV
WAT270946DV 2
186000384DV
186000307C
186000847C
186000327C
186000307DV
186000847DV
186000327DV
186004132C
186004133C
186004168C
1860041123
186002802
186002626
Combination with PE Septum-less Cap
1860026403
1860026393
1860052203
1860052213
186000385C
186003886C
186000385DV
186004167C
Vials Only
Vials Only
186000273
186000848
186000273DV
186000848DV
Max
1100 L
1100 L
280 L
400 L
1100 L
950 L
Residual
750 L
750 L
20 L
300 L
750 L
9 L
Max
1700 L
1700 L
1500 L
290 L
530 L
1700 L
1500 L
Residual
170 L
170 L
22 L
10 L
170 L
170 L
22 L
Deactivated Vials Only
186005219
WAT063300
186002805
WAT063300DV
Inserts
WAT094170(DV)1 WAT094170(DV)1

230 L/20 L
WAT094170(DV)1
230 L/20 L
230 L/20 L
WAT094171(DV)1 WAT094171(DV)1

144 L/6 L
144 L/6 L
186004169
186004169
WAT094171(DV)1
144 L/6 L
Cap and Septum

PE Septumless Caps
186004169
186004169
186004169
Cap Black
WAT058875
Septa Silicone/PTFE
WAT058874
186004169
Screw Cap and Septum Silicone/PTFE

PE Septum-less Cap
186004169
186004169
186004169
186004169
186000274
186004169
Blue LectraBond
186000274
186000274
186000274
186000274
186002129
186000274
Red LectraBond
186002129
186002129
186002129
186002129
186002130
186002129
186002456
Green LectraBond
186002130
186002130
186002130
186002130
White LectraBond
186002456
186002456
186002456
186002456
186002130
Blue LectraBond
186000305
186000305
186000305
186000305
186000305
186000305
Red LectraBond
186002128
186002128
186002128
186002128
186002128
186002128
Green LectraBond
186002127
186002127
186002127
186002127
186002127
186002127
White LectraBond
186002457
186002457
186002457
186002457
186002457
186002457
186000989(DV)1
186003455
Alliance 2690/2695
Alliance 2790/2795
186002456
Screw Cap and Pre-slit Septum Silicone/PTFE
For Dissolution System

Pre-assembled Vial, Cap and Pre-slit Septum
Compatible Systems
70
Septum not bonded.
Vials not certified.
www.waters.com

Snap Cap 12 x 32 mm Vials for Alliance Systems
Clear Glass
Amber
Max Recovery
PP 300 L
PP 750 L
Clear Glass Crimp
Amber Crimp
Total Recovery
24
25
26
27
28
29
30
31
186002642
186005223
186002641
186005222
186002628
186005224
Combination Packs
186000234(DV)1
Vials
Vials Only
WAT094219
WAT094220
186000984
WAT094219DV
WAT094220DV
186000984DV
Max
1100 L
1100 L
280 L
Residual
750 L
750 L
20 L
Max
1700 L
1700 L
1500 L
Residual
170 L
170 L
22 L
WAT094222
WAT094223
186000302
WAT094222DV
WAT094223DV
186000302DV
400 L
1100 L
1100 L
950 L
300 L
750 L
750 L
9 L
290 L
530 L
1700 L
1700 L
10 L
170 L
170 L
170 L
Inserts
WAT094170(DV)1 WAT094170 (DV)1

230 L/20 L
WAT094170(DV)1 WAT094170(DV)1
230 L/20 L
230 L/20 L
WAT094171(DV)1 WAT094171 (DV)1
230 L/20 L
WAT094171(DV)1 WAT094171(DV)1
144 L/6 L
144 L/6 L
144 L/6 L
144 L/6 L
Blue
186000303
186000303
186000303
186000303
186000303
Black
186002649
186002649
186002649
186002649
186002649
186002649
Red
186002650
186002650
186002650
186002650
186002650
186002650
Blue
186000304
186000304
186000304
186000304
186000304
186000304
Black
186002648
186002648
186002648
186002648
186002648
186002648
Red
186002647
186002647
186002647
186002647
186002647
186002647
Blue
186000328
186000328
186000328
186000328
186000328
186000328
Black
186002645
186002645
186002645
186002645
186002645
186002645
Red
186002646
186002646
186002646
186002646
186002646
186002646
Snap Cap and Septum Silicone/PTFE

186000303
Snap Cap and Pre-slit Septum Silicone/PTFE
Snap Cap and PTFE Septum
Crimp Cap
Crimp Cap Silicone/PTFE Septum
PSL404219
PSL404219
Crimp Cap PTFE/Silicone/PTFE Septum
PSL404231
PSL404231
Compatible Systems
Alliance 2690/2695
Alliance 2790/2795

1
When (DV) appears beside the part number, a deactivated version of this
product can be ordered by adding DV to the right of the part number.
Holder for 12 x 32 mm Vials

Description
Holder for12 x 32 mm Vials (5/pk)
Part No.
186004487
(Vials not included)
www.waters.com
71

Vials for Waters 717 Autosampler
4 mL Screw Neck
Amber Screw Neck
Total Recovery
PP Screw Neck Vial
PP Conical
Glass Shell Vial
Amber Glass
Shell Vial
32
33
34
35
36
37
38
186002629C
186004031
WAT025051
WAT025050
2950 L/50 L
2400 L/1600 L
2400 L/1600 L
15 x 45 mm Vials
48 Position Carousel
Combination Packs
Vial, Cap and LectraBond PTFE/Silicone Septum
186000838C
186001133C
186000838DV
186001133DV
Vial, Cap and LectraBond Pre-slit PTFE/Silicone Septum
186000839C
186001134C
186000839DV
186001134DV
186002630C
Vial and PE Snap Cap

Components
Vials Only
186000840(DV)1
186001135(DV)1
186002520
1860009992
2400 L/1600 L
2400 L/1600 L
3000 L/40 L
2000 L/1000 L
186000841
186000841
186000841
186000841
Cap LectraBond PTFE/Silicone 100/pk

Screw Cap with Bonded PTFE/Silicone Septum 1000/pk
186000965
186000965
186000965
186000965
Cap LectraBond Pre-slit PTFE/Silicone 100/pk
186000842
186000842
186000842
186000842
Black Phenol Cap 144/pk
WAT072711
WAT072711
WAT072711
WAT072711
PTFE Septum 1440/pk
WAT073005
WAT073005
WAT073005
WAT073005
PTFE Septum 144/pk
WAT072714
WAT072714
WAT072714
WAT072714
Self Sealing Septum 144/pk
WAT022861
WAT022861
WAT022861
WAT022861
WAT072704(DV)1
WAT072704(DV)1
244 L/6 L
244 L/6 L
WAT015199(DV)1
WAT015199(DV)1
230 L/20 L
230 L/20 L
250 L Plastic Conical Insert (PMP) 144/pk3
WAT072030
WAT072030
230 L/20 L
230 L/20 L
Springs for LVI 100/pk
WAT072708
WAT072708
186001729
186001729
250 L Glass Insert3

250 L Glass Insert 144/pk3
250 L PP Insert 1000/pk
WAT072704
2 Item contains 1,000 vials
3 Inserts requires springs (Part No. WAT072708)
1 mL Shell
Amber
Total Recovery
PP Conical
39
40
41
42
8 x 40 mm Vials
96 Position Carousel
Vials for 96 Position Carousel

Shell Vial and Snap Cap
WAT025054C
WAT025053C
186000837C
Shell Vial and Snap Cap Deactivated
WAT025054DV
WAT025053DV
186000837DV
Pack Size
150 L Glass Insert
WAT022476
250
250
100
100
600 L/400 L
600 L/400 L
700 L/6 L
650 L/50 L
WAT072294(DV)1
WAT072294(DV)1
WAT072289
WAT078515
WAT078515
Spring for LVI
WAT072289
144 L/6 L
144 L/6 L
PE Snap Cap 1000/pk
WAT078515
WAT078515
200 L PE Grad Insert with Poly Spring 1000/pk
186001728
186001728
1 mL Shell Vial Assembled for Dissolution System, 500/pk
WAT022479
When (DV) appears beside the part number, a deactivated version of this
product can be ordered by adding DV to the right of the part number.
Vials not certified.
72
www.waters.com

Clear
Amber
Max Recovery
Amber Max
300 L PP
43
44
45
46
47
600000668CV
600000669CV
600000670CV
600000755CV
186000307C
186000847C
186000327C
186003886C
186000307DV
186000847DV
186000327DV
186000272C
186000846C
186000326C

186003885C
Combination Packs
186002639
186002640

Max
1600 L
1600 L
1100 L
1100 L
240 L
Residual
150 L
150 L
10 L
10 L
10 L
WAT094171
WAT094171
144 L/6 L
144 L/6 L
Components
Plates for Waters 2707 Autosampler

96-Well Plates
Plate
384-Well Plates
186002643
186002481
186002482
186002632
100
50
50
50
50
350 L
800 L
2 mL
250 L
100 L
186002483
186002483
186002484
186002788
186002788
186002788
186002788
186002788
186002789
186002789
186002789
186002789
186002789
125 L
40 L
60 L
40 L
40 L
Part No.
Max Volume
Residual Volume
15 L
Pack Size
Well Volume
186002631
Sealing Options
2707 Residual Volume

Description (For Metal-Tipped Needles ONLY)
1860014381
186001437 (DV)2
650 L
186001436 (DV)2
850 L
15 L
186000349 (DV)2
650 L
15 L
186000855 (DV)2
850 L
15 L
186000857
186000856
10 ml Vial for Waters 2707 Autosampler

10 mL Screw Neck
Components
22 x 45 mm Clear Glass Vial
186001420
Cap with x-slit PTFE Silicone Septa
186004632
www.waters.com
48
73

Screw Cap 12 x 32 mm Vials for Compatible Systems
Clear
Amber
Amber Max
Recovery
Clear Glass
Max Recovery
Qsert Vial
Amber Qsert
PP 300 L
PP 750 L
10 mm Cap
PP 250 L
8 mm Cap
49
50
51
52
53
54
55
56
57
58
186000326C
186001126C
186001130C
WAT270946C1
186000326DV
186001126DV
186001130DV
WAT270946DV1
186000327C
186001128C
186001131C
186000327DV
186001128DV
186001131DV

600000751CV
Vial, Cap and Pre-slit

Silicone/PTFE Septum
600000752CV 600000754CV 600000749CV
600000668CV 600000669CV 600000755CV 600000670CV

186000272C
186000846C
186000272DV 186000846DV
Bonded Pre-slit
186000307C
186000307DV 186000847DV
186000847C
186003885C
186003886C
Combination Packs
186002640
186005220
186002639
186005221
186002626
186005219
Vials Only
186000273
Vials Only
186000848
186002802
186002804
186002803
186000273DV 186000848DV
WAT063300
WAT094172
WAT063300DV
Inserts
300 L with Poly Spring Deactivated
150 L with Poly Spring Deactivated
WAT094170
WAT094170
WAT094170
WAT094170DV
WAT094170DV
WAT094170DV
WAT094171
WAT094171
WAT094171
WAT094171DV
WAT094171DV
WAT094171DV
186004169
186004169
Cap and Septum

PE Septumless Caps
186004169
186004169
186004169
186004169
186004169
Black Cap
WAT058875
Cap and Septum, Silicone/PTFE,

Assembled
WAT094174
Septum Only, PTFE/Silicone, Pre-slit
WAT058876
Septum Only, Silicone/PTFE
WAT058874
Septum Only, PTFE
WAT210685
WAT058886
Screw Cap and Septum Silicone/PTFE

186000274
Blue LectraBond
186000274
186000274
186000274
186000274
186000274
186000274
Red LectraBond
186002129
186002129
186002129
186002129
186002129
186002129
186002129
Green LectraBond
186002130
186002130
186002130
186002130
186002130
186002130
186002130
Screw Cap and Pre-slit Septum Silicone/PTFE

Blue LectraBond
186000305
186000305
186000305
186000305
186000305
186000305
186000305
Red LectraBond
186002128
186002128
186002128
186002128
186002128
186002128
186002128
Green LectraBond
186002127
186002127
186002127
186002127
186002127
186002127
186002127
Compatible Systems
Agilent Technologies
Alcott, Antek, CTC, Spark
Thermal Separations
Beckman, Dynatech, Finnigan,
Fisons, Gilson
Hitachi, LDC, Perkin-Elmer, Shimadzu,

Spectra-Physics, Thermo, Varian
74
Septum not bonded.
www.waters.com

Snap and Crimp Cap 12 x 32 mm (9 mm Cap) Vials for Compatible Systems
Clear
Amber
Max Recovery
Qsert Vial
PP 300 L
PP 750 L
Clear Crimp
Amber
Crimp
59
60
61
62
63
64
65
66
186001124(DV)1
186002642
186005223
186002641
186005222
186002628
186005224
Combination Packs
Vial, Cap and Pre-Slit
186001125(DV)
Vial, Cap and PTFE Septum
186001127(DV)
Vials Only
Vials Only
WAT094219
WAT094220
186000984
WAT094219DV
WAT094220DV
186000984DV
WAT094222
WAT094223
WAT094222DV
WAT094223DV
WAT094170(DV)1
WAT094171(DV)1
WAT094170(DV)1
WAT094170(DV)1
WAT094170(DV)1
WAT094171(DV)1
WAT094171(DV)1
WAT094171(DV)1
Blue
186000303
186000303
186000303
186000303
186000303
Black
186002649
186002649
186002649
186002649
186002649
186002649
Red
186002650
186002650
186002650
186002650
186002650
186002650
Crimp Cap Silicone/PTFE Septum
PSL404219
PSL404219
Crimp Cap PTFE/Silicone/

PTFE Septum
PSL404231
PSL404231

Inserts
Snap Cap and Septum Silicone/PTFE

186000303
Snap Cap and Pre-slit Septum Silicone/PTFE

Blue
186000304
186000304
186000304
186000304
186000304
186000304
Black
186002648
186002648
186002648
186002648
186002648
186002648
Red
186002647
186002647
186002647
186002647
186002647
186002647
Blue
186000328
186000328
186000328
186000328
186000328
186000328
Black
186002645
186002645
186002645
186002645
186002645
186002645
Red
186002646
186002646
186002646
186002646
186002646
186002646
Snap Cap and PTFE Septum
Crimp Cap
Compatible Systems
Agilent Technologies, Beckman,
Dynatech, Finnigan, Fisons,
Gilson, Hitachi, LDC, Perkin-Elmer,
Shimadzu, Spectra-Physics, Varian
CTC, Spark, Thermal Separations

1
Holder for 12 x 32 mm Vials

Description
Part No.
Holder for12 x 32 mm Vials (5/pk)
186004487
(Vials not included)
www.waters.com
75

15 x 45 mm Vials for Compatible Systems
4 mL Screw Neck
Amber
Screw Neck
Total Recovery
PP Screw
Neck Vial
PP Snap Cap
Glass Shell Vial
Amber Glass
Shell Vial
67
68
69
70
71
72
73
186002629C
186004031
WAT025051
WAT025050
15 x 45 mm Vials and Accessories
Combination Pack
Vial, Cap and LectraBond PTFE/Silicone Septum
186000838C
186001133C
186000838DV
186001133DV
Vial, Cap and LectraBond Pre-slit PTFE/Silicone Septum
186000839C
186001134C
186000839DV
186001134DV
186002630C
Vial and PE Snap Cap

Components
Vials Only
186000840
186001135
186000840DV
186001135DV
1860009991
186002520
LectraBond Cap and Septum

Black Cap PTFE/Silicone 100/pk
186000841
186000841
186000841
Screw Cap with Bonded PTFE/Silicone Septum 1000/pk
186000965
186000965
186000965
Black Cap Pre-slit PTFE/Silicone 100/pk
186000842
186000842
186000842
186000965
Caps, Septa, and Inserts

Black Phenol Cap 144/pk
WAT072711
WAT072711
WAT072711
PTFE Septum 144/pk
WAT073005
WAT073005
WAT073005
PTFE Septum 144/pk
WAT072714
WAT072714
WAT072714
Self Sealing Septum 144/pk
WAT022861
WAT022861
WAT022861
250 L Glass Insert
WAT072704
WAT072704
WAT072704
WAT072704DV
WAT072704DV
WAT072704DV
WAT015199
WAT015199
WAT015199
WAT015199DV
WAT015199DV
WAT015199DV
250 L Plastic Conical Insert (PMP) 144/pk
WAT072030
WAT072030
WAT072030
Springs for LVI 100/pk
WAT072708
WAT072708
WAT072708
250 L PP Insert 100/pk
186001729
186001729
186001729
4 mL Screw Cap
10 mL Screw Neck
250 L Glass Insert Deactivated

250 L Glass Insert 144/pk
250 L Glass Insert 144/pk Deactivated
Compatible Systems
Bruker, Kontron, Perkin-Elmer, Shimadzu, Tosoh, Unicam
1
Item contains 1,000 vials.
GPC 2000 Vials
Aqua Analysis System Vials

Components
74
75
Vial
186000840
186001420
Black Screw Cap
600000162
186001421
PTFE Septum
WAT0727141
186001422
Components
Item contains 144 vials.
76
22 mL Vial with pre-slit silicone/PTFE septum, 100/pk
186004108
Solid Cap, PTFE/silicone liner, 100/pk.

Solid cap for shipping water samples.
186004109
Mailing Box dor 22 mL vials, 100/pk.

Box for shipping samples to lab.
186004111
www.waters.com

Settings for Alliance 2690 & 2695 Vials AND Low Volume Inserts (LVI)
T he Waters 2690 Separations Module is set initially to accept vials with a bottom thickness <1.6 mm. Any vial that does not meet this criteria should not
be used without first adding a positive needle offset to the sample draw depth. Failure to do so can cause vial breakage or needle damage.
Vial Column Number*
Average Thickness
Needle Offset
Notes*
Comments
Screw Cap Glass Vial
Description
0.037 (0.93 mm)
1,3
Add at least 1 mm offset when used with LVI
1,3
0.037 (0.93 mm)
11
Polypropylene Screw Neck Vial (300 L)
0.037 (0.93 mm)
1 mm
27
Polypropylene Snap Cap Vial (300 L)
0.037 (0.93 mm)
1 mm
22
Total Recovery Vial
0.037 (0.93 mm)
31
Total Recovery Vial
0.037 (0.93 mm)
21
0.037 (0.93 mm)
1,3
24
Snap Cap Glass Vial
0.063 (1.59 mm)
1,3
29
Crimp Cap Vial
0.068 (1.72 mm)
1 mm
2,3
Variable thickness; Add at least 1 mm offset when used with LVI
58
Screw Cap V Vial
0.058 (1.46 mm)
Low volume (250 L) vial
24
Low Volume Insert (300 L)
0.024 (0.61 mm)
Use with vials with neck opening, 6 mm
29
Low Volume Insert (150 L)
0.028 (0.71 mm)
Use with vials with neck opening, 6 mm
0.062 (1.57 mm)
LVI (300 L) in Screw
21
Cap Vial (7 mm neck)
LVI (150 L) in Screw
21
Cap Vial (7 mm neck)
Recommended configuration for this LVI
0.065 (1.65 mm)
1 mm
0.090 (2.27 mm)
1 mm
Recommended configurationhas the lowest sample

volume requirement
29
LVI (300 L) in Snap/Crimp
30
Vial (6 mm neck)
30
LVI (150 L) in Snap/Crimp
30
Vial (6 mm neck)
30
Snap/Crimp Vial
0.068 (1.72 mm)
1 mm
2,3
Variable thickness; Add at least 1 mm offset when used with LVI
15
Snap Cap Vial
0.063 (1.59 mm)
1,3
Variable thickness. Add at least 1 mm offset
0.091 (2.30 mm)
1 mm
Variable thickness. Add at least 1 mm offset
* For more details, see vials descriptions on pages 78 and 79.
*Notes:
1. Clears needle tipno offset required. Meets the criteria of a bottom thickness < 1.6 mm.
2. Does not clear needle tippositive needle offset should be used.
3. Designed to accept Waters low volume inserts (LVIs)at least 1 mm offset should be added.
4. T his dimension should be added to vial bottom thickness and result checked against the criteria for bottom thickness.
Literature
References
Sample Vials & Accessories Brochure,
www.waters.com

77

Vials for Alliance Systems
Most Commonly Used Vials for Alliance Systems
Vials for Waters 717 Autosampler: 15 x 45 mm Vials
Clear 12 x 32, Type 1, 33-Expansion Glass, Screw Neck with Quick Thread Design
32
Clear 15 x 45, Type 1, 33-Expansion Glass, Screw Neck Vial.
Amber 12 x 32, Type 1, 51-Expansion Glass Screw Neck with Quick Thread Design
33
Amber 15 x 45, Type 1, 51-Expansion Glass, Screw Neck Vial.
Clear Maximum Recovery Vial 12 x 32, Type 1 33-Expansion Glass, Screw Neck with
Quick Thread Design (6 mm Opening, 9 mm Cap).
34
Total Recovery Screw Neck Vial Clear Glass 15 x 45, Type 1, 33-Expansion Glass.
10
35
Polypropylene 15 x 45, 3 mL Round Bottom Screw Neck Vial 1,000/pk.
11
Polypropylene 12 x 32, 300 L Screw Neck with Quick Thread Design (6 mm Opening,
9 mm Cap). Reformulate Clean PP Vial.
36
Polypropylene Snap Cap Vial with Conical Bottom, PE Snap Caps.
12
Clear 12 x 32, Type 1, 33-Expansion Glass, Screw Neck (6 mm Opening, 10 mm Cap).
37
4 mL Glass Shell Vial with Polyethylene Snap Cap, Type 1, 51-Expansion Glass.
13
Total Recovery Vial Clear 12 x 32, Type 1, 33-Expansion Glass, Screw Neck with
38
4 mL Amber Shell Vial with Polyethylene Snap Cap, Type 1, 51-Expansion Glass.
14
Amber Maximum Recovery Vial, 12 x 32, Type 1 51-Expansion Glass, Screw Neck with
39
1 mL Clear Glass Shell Vial (8 x 40 mm) Type 1, 51-Expansion Glass with

Polyethylene Snap Cap 250/pk.
15
40
1 mL Amber Glass Shell Vial (8 x 40 mm) Type 1, 51-Expansion Glass with

Polyethylene Snap Cap, Type 1, 250/pk.
41
Total Recovery Clear Glass Vial (8 x 40 mm) with Polyethylene Snap Cap,
Type 1, 51-Expansion Glass.
42
650 L Polypropylene Vial (8 x 40 mm) with Polyethylene Snap Cap.
Screw Cap 12 x 32 mm Vials for Alliance Systems
Vials for Waters 717 Autosampler: 8 x 40 mm Vials
16
17
18
Clear Maximum Recovery Vial 12 x 32, Type 1 33-Expansion Glass, Screw Neck with
43
19
44
20
45
46

47

48
Clear 22 x 45 mm TYpe I, 33-expansion glass screw neck vial
21
22
Total Recovery Vial Clear 12 x 32, Type 1, 33-Expansion Glass, Screw Neck with
23
Amber Maximum Recovery Vial, 12 x 32, Type 1 51-Expansion Glass, Screw Neck with
Snap Cap 12 x 32 mm Vials for Alliance Systems
Vials for Compatible Systems
24
Clear 12 x 32, Type 1, 33-Expansion Glass, Snap Cap Vial (6 mm Opening, 9 mm Cap).
25
Amber 12 x 32, Type 1, 51-Expansion Glass Snap Cap Vial (6 mm Ppening, 9 mm Cap).
26
Clear Maximum Recovery Vial 12 x 32, Type 1, 33-Expansion Glass, Snap Cap Vial
49
27
Polypropylene 12 x 32, 300 L Snap Cap Vial (6 mm Opening, 9 mm Cap).

Reformulate Clean PP Vial.
50
28

51
Amber Maximum Recovery Vial 12 x 32, Type 1, 51-Expansion Glass, Screw Neck with
29
Clear 12 x 32, Type 1, 33-Expansion Glass, Crimp Top Vial (6 mm Opening, 12 mm Cap).
52
30
Amber 12 x 32, Type 1, 51-Expansion Glass, Crimp Top Vial (6 mm Opening, 12 mm Cap).
Clear Maximum Recovery Vial 12 x 32, Type 1, 33-Expansion Glass, Screw Neck with
53
31
Total Recovery Vial Clear 12 x 32, Type 1, 33-Expansion Glass, Snap Cap Vial
Qsert Vial Clear Screw Cap Glass Vial, Quick Thread Design with Fused in Glass Insert
54
Qsert Vial Amber Screw Cap Glass Vial, Quick Thread Design with Fused in Glass Insert
55
56
57
58
Polypropylene 12 x 32, 250 L Screw Neck Vial (6 mm Opening, 8 mm Cap).
78
Screw Cap 12 x 32 mm Vials for Compatible Systems
www.waters.com
Snap and Crimp Cap 12 x 32 mm (9 mm Cap) Vials for Compatible Systems

59
15 x 45 mm Vials for Compatible Systems: Vials and Accessories
Clear 12 x 32, Type 1, 33-Expansion Glass, Snap Cap Vial (6 mm Opening, 9 mm Cap).
67
Clear 15 x 45, Type 1, 33-Expansion Glass, Screw Neck Vial.
60
Amber 12 x 32, Type 1, 51-Expansion Glass Snap Cap Vial (6 mm Opening, 9 mm Cap).
68
Amber 15 x 45, Type 1, 51-Expansion Glass, Screw Neck Vial.
61
Maximum Recovery Vial 12 x 32, Type 1, 33-Expansion Glass, Snap Cap Vial
6 mm Opening, 9 mm Cap).
69
Waters Total Recovery Screw Neck Vial Clear Glass 15 x 45, Type 1, 33-Expansion Glass.
62
Qsert Vial Clear Snap Cap Glass Vial with Fused in Glass Insert (6 mm Opening,
9 mm Cap).
70
Polypropylene 15 x 45, 3 mL Screw Neck Vial.
71
Polypropylene Snap Cap Vial with Conical Bottom, PE Snap Caps.
63

72
4 mL Glass Shell Vial with Polyethylene Snap Cap, Type 1, 51-Expansion Glass.
64

73
4 mL Amber Shell Vial with Polyethylene Snap Cap, Type 1, 51-Expansion Glass.
65
Clear 12 x 32, Type 1, 33-Expansion Glass, Crimp Top Vial (6 mm Opening, 12 mm Cap).
66
Amber 12 x 32, Type 1, 51-Expansion Glass, Crimp Top Vial (6 mm Opening, 12 mm Cap).
15 x 45 mm Vials for Compatible Systems: GPC 2000 Vials

74
4 mL Glass Screw Neck Vial, Type 1, 33-Expansion Glass for GPC 2000.
75
10 mL Screw Neck Glass Vial for GPC 2000.
Vials Troubleshooting Guide

Waters offers solutions that help eliminate common problems that conventional sample vials have been known to cause in the laboratories.
Problem
Septum dislodged during shipment or use
Vacuum forms in vial during sample draw
Impact
Sample-limited applications require the use of

cumbersome low-volume inserts
Need to perform multiple injections with minimum residual

volume in each vial requires LVI to obtain minimum residual
volume, but maximum capacity is only 300 L
Need to use glass inserts in a 96-well plate format but it
requires capping each insert one-at-a-time
Frequent needle damage
Analyte compounds are sticking to the glass

surface of the vial
Inconsistent quality between laboratory sites
Sample spill over

Sample draw reproducibility problems
Increased labor required for inserting the LVI into the
vial leads to delays in sample processing
Increased labor time and difficulty when pipetting into
small neck opening of LVI
Additional handling increases chance of contamination
Increased costs from purchasing multiple components:
vial, cap and LVI
Increased labor to fill additional sample vials

Increased cost to purchase additional sample vials and LVIs
Delay in sample processing
Downtime causing missed deadlines

Cost of repairs
Laboratory owns HPLC instruments from several

different manufacturers
Need to insert septum or need to rerun analysis

Loss of time
Purchasing several different vials

Increased number of purchase orders
Unable to take advantage of quantity discounts, leading
to higher costs
Loss of sample
Loss of time
Need to run the analysis again
Solution
Check to see if needle is piercing in center of septa.

Check to see if needle is sharp.
Use pre-slit septa which provides proper venting,

eliminating sample spill over and insuring reproducible
sample draw volumes.
Use Waters Total Recovery vial and Maximum Recovery vial:

No need to use LVIs.
Wide neck opening for easy sample pipetting.
One less handling step reduces chance of contamination.
Only need one component, saving storage space and costs.
Use Waters Total Recovery vial and Maximum Recovery vial:

The increased capacity and low residual volume allows you
to perform multiple injections with minimum residual volume
in a single vial.
The glass inserts in the Waters 96-well format allows for the
use of a sealing cap mat, saving time and labor.
All Waters vials have dimensional specifications that

eliminate the potential of needle damage.
The tight dimensional tolerances on all Waters vials and

accessories make them ideal for use with virtually all
HPLC systems.
Reduce the number of purchase orders and take advantage of
quantity discounts by buying all your sample vials from Waters.
Deactivated glass vials and inserts: Waters uses a gas phase

deactivation process that renders the glass surface inert. Unlike
other deactivated vials, the surface modification is permanent,
resulting in an indefinite shelf life.
Waters vials are distributed world wide from the same source.
Beware of Poor Quality Look-alike Vials
Only Waters Alliance Total Recovery vials and Maximum Recovery vials utilize a proprietary manufacturing process,
ensuring that the slope of the internal taper will deliver all of the sample to the bottom of the vial.
T he bottom thickness is held to a close tolerance eliminating needle damage caused by bottoming out.
www.waters.com
79
FILTRaTION
Sirocco Protein Precipitation Plate
Protein Precipitation Plate
State-of-the-art design for maximum analyte recovery
Typical Plasma Precipitation
Unique valve design prevents flow until you are

ready to process
Eliminates time-consuming centrifugation

and pipetting steps
1. Place the Sirocco PPT Plate on a collection plate, this suspends

the valve mat tips in the center of the collection plate wells
during processing.
2. Add crash solvent to the wells.
Design ensures no sample cross talk
3. Add plasma.
Maximize mass spec uptime by

eliminating cloudy filtrates
4. Apply the vented cap mat.
Low extractables, no interferences

in UV or ion suppression
6. Filter on a vacuum manifold at 8-10 Hg or centrafuge.
5. Vortex the filter plate/collection plate stack.
Waters, in collaboration with Pall Life Sciences, is pleased to introduce the

most technologically-advanced protein precipitation plate on the market.
T he Sirocco Protein Precipitation ( P PT ) Plate enables high-throughput
in-well protein precipitation of biological samples without the fear of
cloudy filtrates, clogged devices, cross-talk, or leaking during use. T his
is due to a unique filter system, sealing cap mat and patented valve
tec hnology. It provides optimum performance in faster processing time
by reducing steps in in-well sample processing and complete recovery
of clean filtrate from smaller plasma sample volumes.
Sirocco Patented Plate Technology
T his patented plate technology includes:
Valve mat on the tips of the wells to prevent flow or

drips from the plate until vacuum is applied
Gradient of purpose-selected filtration media for clean,

precipitate free filtration
Vented cap mat for processing during mixing and vacuuming
T he plate was validated for performance using a variety of polar and

non-polar acids, bases, and neutral compounds spiked into porcine and rat
plasmas looking for high recoveries. T he plates are highly reproducible and
linear, with the analytes examined having >95% recovery.
All materials used in the Sirocco plate were chosen after careful and
intensive evaluation for:
Ion suppression
MS extractables
Ion enhancement
Recovery of analytes
UV extractables
80
Filtration
Typical processing of 96 samples takes less than 15 minutes. Note, there

is no need to remove the cap or valve mat. T he slits in the valve tips are
designed to open under several inc hes of Hg vacuum. T he cap mat is
pre-slit to allow processing steps of vortexing and vacuum filtration. T he
entire Sirocco plate assembly is disposed of after the vacuum filtration step.
It is designed for in-well sample preparationno mat removal is required;
therefore, the risk of cross-talk is reduced or eliminated, as compared to
other commercially available plates.
Specification
T he Sirocco plate has 1 ml sample wells. With the cap mat applied, the
combined plasma/crash solvent volume should not exceed 600 L. T his
allows enough volume above the sample to ensure complete mixing during
the vortex step. T he package contains plates assembled with a valve mat,
and pre-slit cap mats. T here are no other parts required for the in-well
processing of plasma.
T he Sirocco Protein Precipitation Plate is suitable for automated processing.
Description
Qty.
Part No.

1/pk
5/pk
186003873
186002448
For more information visit us at www.waters.com/sirocco
www.waters.com
FILTRaTION
Pall Life Sciences Sample and Solvent Filtration Products

Concerned about particulate matter in your sample?
Step 1: W hat is the nature of your sample?
Nature
of
Sample
Aqueous
GHP* universal
Nylon
PVDF
PES
Filtration of samples and solvents is a preventative maintenance procedure

that saves lab time and money. Filtration provides immediate protection for
the components of column and instrumentation by minimizing down time.
Pall Life Sciences filters have been Certified for Compliance; which means
they have been designed and developed to assist customers in complying
with their regulatory and quality objectives.
Waters carries a broad range of Pall Life Sciences filter products, a range of
different membranes for solvent and sample compatibility, and a variety of
devices for various filtration applications.
Choosing the right filter for your application.
To choose the right filter you need to consider sample characteristics,
volume, pore size and decide if the sample may require prefiltration
because it is laden with particulate matter.
Ion
Chromatography
IC
Non Polar
CR/
PTFE
GHP
Protein
GHP
GH
PVDF
For samples with laden particulate that are difficult to filter, it is best to use a syringe filter with a glass fiber
pre-filter over the membrane. These are available in GHP and Nylon.
Step 2: W hat micron size are the particles

in the column are you using?
Column
Pore Size of Filter
> 3 m
0.45 m
< 3 m
0.20 m
Membrane Choices
GHP Acrodiscs
Step 3: W hat is the volume of your sample?
Hydrophilic propylene membrane suitable for

aqueous, organic and has low protein binding.
Volume
Acrodisc Size
Nylon Acrodiscs
< 2 mL
4 mm
< 10 L
< 10 mL
13 mm minispike
< 14 L
< 10 mL
13 mm male luer
< 30 L
< 100 mL
25 mm
< 100 L
Hydrophilic nylon membrane
GHP Acrodics GF and Nylon Acrodics GF
Designed with a glass fiber prefilter over the membrane

for hard to filter samples laden with particulate matter.
Hold Up Volume
Glass Fiber Acrodiscs
Can be used alone or as a prefilter with

another Acrodics in series.
Acrodisc LC (PVDF)
Hydrophilic polyvinylidene fluoride good for

aqueous and organic solvents.
Example 1: 1.5 mL of aqueous sample to be filtered

for injection on a 5 m column:
Step
Question
Answer
Choice
Sample
Aqueous
GHP and others
Used for aggressive organic solvents
Particle size in column
5 m
0.45 m
Ion Chromatography (IC) Acrodisc
Volume
1.5 mL
4 mm or larger
Acrodisc CR(PTFE)
Certified to contain low ionic backgrounds

Supor (PES)
Hydrophilic polyethersulfone for biological, pharmaceutical
or sterilizing. Can be gamma sterilized or autoclaved.
www.waters.com
Choice: Membrane 0.45 m GHP Acrodisc in 4 mm or larger. You can

also use the Nylon, PVDF or PES (other choices of hydrophilic membranes
under the aqueous sample path). In terms of device size, if you are injecting only a few uL of sample on the column, you can use any device size.
T he 13 and 25 mm Acrodiscs have hold up volumes of at most 100 L,
leaving plenty of filtered sample for the application.
Filtration
81
FILTRaTION
Acetone
Acetoniltrile
Acetic acid, glacial
n-Butanol
Chloroform
Dioxane
Dimethyl formamide
Dimethyl sulfoxide
Ethanol
Ethyl acetate
Ethyl ether
Freon TF
Hydrochloric acid (1N)
Hexane, dry
Methanol
Methylene chloride
Methyl ethyl ketone
N-Methylpyrrolidone
Isopropanol
Sodium hydroxide (5N)
Tetrahydrofuran
Tetrahydrofuran/
water (50/50)
Toluene
Water
Filter Design and Membrane Choices
GHP Acrodisc 13 (13 mm)
GHP Acrodisc (25 mm)
GHP Acrodisc GF (25 mm)
R
LR
NR

= RESISTANT
= LIMITED RESISTANCE
= NOT RESISTANT
= INSUFFICIENT DATA
GH Polypro Syringe Filters
PTFE Syringe Filters

Acrodisc 4CR PTFE (4 mm)
R*
LR
R*
R*
R*
LR
R*
R*
LR
LR
LR*
Acrodisc 13CR PTFE (13 mm)
R*
R*
R*
R*
R*
R*
R*
Acrodisc CR PTFE (25 mm)
R*
R*
R*
R*
R*
R*
R*
PVDF Syringe Filters

Acrodisc LC13 PVDF (13 mm)
NR*
NR*
NR*
R*
NR*
NR*
NR
R*
Acrodisc LC PVDF (25 mm)
NR*
NR*
NR*
R*
NR*
NR*
NR
R*
Nylon Acrodisc 4 (4 mm)
R*
NR
R*
R*
R*
NR
NR
NR
R*
R*
NR
LR
R*
Nylon Acrodisc 13 (13 mm)
R*
NR
R*
R*
R*
NR
NR
NR
R*
R*
NR
LR
R*
Nylon Acrodisc (25 mm)
R*
NR
R*
R*
R*
NR
NR
NR
R*
R*
NR
LR
R*
Nylon Acrodisc GF (25 mm)
R*
NR
R*
R*
R*
NR
NR
NR
R*
R*
NR
LR
R*
NR
LR
NR
NR
NR
NR
LR
LR
LR
NR
NR
NR
LR
NR
NR
NR
NR
NR
NR
NR
NR
NR
LR
NR
NR
NR
NR
NR
NR
NR
GH Polypro
FP Verciel (PVDF)
NR
LR
NR
NR
LR
NR
NR
LR
Nylaflo (Nylon)
NR
NR
LR
NR
LR
NR
NR
NR
TF (PTFE)
Nylon Syringe Filters
Ion Chromatography Syringe Filters

IC Acrodisc (13 mm & 25 mm)
Glass Fibre Syringe Filters

GF Acrodisc
Acrylic Copolymer Syringe Filters

Non-sterile Acrodisc (25 mm)
Disc Filters
Note:
R = Resistant
No significant change was observed in flow rate or bubble point of the membrane.
4 mm Polypropylene
Housing Male Luer
13 mm Polypropylene
Housing Male Luer
* UV absorbance was set at 254 nm

LR = Limited Resistance
Moderate changes in physical properties or dimension of the membrane were observed.
The filter may be suitable for short term, non-critical use at room temperature.
NR = Not Resistant
The membrane is basically unstable. In most cases, extensive shrinkage or swelling occurs.
The filter may gradually weaken or partially dissolve after extended exposure.
82
Filtration
13 mm Polypropylene
Housing Minispike
25 mm Polypropylene
Housing Male Luer
www.waters.com
FILTRATION
Part Number Cross Reference
Pall Life Sciences
Description
PTFE, 4 mm, 0.45 m
PTFE, 13 mm, 0.2 m
PTFE, 13 mm, 0.45 m
PTFE, 25 mm, 0.2 m
PTFE, 25 mm, 0.45 m
PVDF, 13 mm, 0.45 m
PVDF, 25 mm, 0.45 m
GHP, 13 mm, 0.45 m
GHP, 25 mm, 0.45 m
Nylon, 13 mm, 0.2 m
Nylon, 13 mm, 0.45m
Nylon, 25 mm, 0.2 m
Nylon, 25 mm, 0.45 m
Supor, 25 mm, 0.2 m
PVDF, 47 mm, 0.45 m
PVDF, 47 mm, 0.2 m
Nylon, 47 mm, 0.45 m
Nylon, 47 mm, 0.2 m
PTFE, 47 mm, 0.45 m
PTFE, 47 mm, 0.2 m
GHP, 47 mm, 0.45 m
Supor, 47 mm, 0.2 m
Supor, 47 mm, 0.45 m
Supor, 13 mm, 0.45 m
Glass Filter Holder
Forceps
300 mL Funnel
Glass Base, Tabulated Cap
Ground Joint Flask
Thick Glass Prefilter
AVE Glass Prefilter
Solvent Filtration Membranes
Whatman
Part No.
Millipore
Part No.
Waters
Part No.
6783-0404
6783-1302
6783-1304
6784-2502
6784-2504
6792-1304
6784-1304
6785-2504
6790-1302
6790-1304
6780-2502
7404-004
7402-004
7585-004
7582-004
7002-0447
SJFHL04NS
SJFG013NS
SJFH013NS
SLFG025NS
SLSR025NS
SJHV013NS
SLHV025NS
SJFH013NS
SLCR025NS
SLGS025OS
HVLP04700
FHLP04700
FGLP04700
FHLC04700
GSTF04700
HATF04700
HATF01300
XX1504700
XX6200006
XX1004704
XX1504702
XX1504705
AP2501000
AP4001000
WAT200508
WAT200506
WAT200502
WAT200504
WAT200500
WAT200512
WAT200510
WAT200516
WAT200514
WAT200524
WAT200520
WAT200522
WAT200518
WAT200529
WAT200530
WAT200531
WAT200532
WAT200533
WAT200534
WAT200535
WAT200537
WAT200539
WAT200538
WAT200540
WAT200543
WAT200544
WAT200545
WAT200546
WAT200547
WAT200541
WAT200542
Waters Filter Selector

T he Waters Filter Selector helps you select the most appropriate
filter for your analysis. Simply answer 3 easy questions about
particle size, sample volume, and sample type and we will identify
the most suitable filter.
Description
Qty.
Diameter
Pore Size
Part No.
PVDF Filter
Nylon Filter
PTFE Filter
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
47 mm
47 mm
47 mm
13 mm
47 mm
47 mm
13 mm
47 mm
47 mm
47 mm
47 mm
47 mm
10 mm
10 mm
0.45 m
0.45 m
0.45 m
0.45 m
0.45 m
0.45 m
0.45 m
0.2 m
0.2 m
0.2 m
0.2 mm
0.2 m
1.0 m
1.0 m
WAT200530
WAT200532
WAT200534
WAT200536
WAT200537
WAT200538
WAT200540
WAT200531
WAT200533
WAT200535
186003524
WAT200539
WAT200541
WAT200542
GH Polypro Filter
Supor (PES) Filter
PVDF Filter
Nylon Filter
PTFE Filter
GHP
Supor (PES) Filter
Thick Glass Filter
A/E Glass Filter
Solvent Filtration Apparatus

T he 300 mL capacity 47 mm Glass Filter Funnel and 1 Litre capacity
47 mm Glass Funnel/Support Assembly are ideal for vacuum filtration
of liquids and degassing of HPLC solvent and mobile phases. T he 100%
borosilicate glass construction assures resistance to even the most
aggressive solvents.
Funnel, 300 mL
WAT200545
Glass Base,
tabulated cap
WAT200546
All Glass Filter Holder

47 mm, complete
WAT200543
Ground
Joint Flask
WAT200547
Description
For more information about Waters Filter Selector, please visit:
www.waters.com/filterselector
Part No.
Solvent Filtration Apparatus 110 V, 60 Hz

Solvent Filtration Apparatus 220 V, 50 Hz
All Glass Filter Holder 47 mm, complete
Forceps, SS
Funnel, 300 mL
Glass Base, tabulated cap
Ground Joint Flask
Swinney Holder
Vacuum Pump 110 V, 60 Hz
WAT085113
WAT085102
WAT200543
WAT200544
WAT200545
WAT200546
WAT200547
WAT200566
WAT085114
WAT085123
WAT085115
www.waters.com
Filtration
83
FILTRaTION
Syringe Filters Product List
Acrodisc 13 mm
Pack Size
100
0.2 m
300
1000
100
0.45 m
300
1000
Aqueous
NYLON
PVDF
WAT200524
WAT200806
WAT200525
WAT200807
WAT200834
WAT200520
WAT200512
WAT200521
WAT200513
WAT200832
WAT200827
Non Polar
CR
WAT200506
WAT200507
WAT200823
WAT200502
WAT200503
WAT200821
Protein
PVDF
WAT200806
WAT200807
WAT200512
WAT200513
WAT200827
Ion Chromatography
IC
WAT200810
WAT200811
WAT200844
WAT200812
WAT200813
WAT200842
100
0.2 m
300
1000
100
0.45 m
300
1000
Acrodisc 13 mm Mini spike

Pack Size
Aqueous
GHP
NYLON
PVDF
WAT097962
WAT200562
WAT200804
WAT097963
WAT200563
WAT200805
186004567
WAT200835
WAT200838
WAT200516
WAT200564
WAT200560
WAT200517
WAT200565
WAT200561
WAT200830
WAT200836
WAT200828
Non Polar
CR
GHP
WAT200556
WAT097962
WAT200557
WAT097963
WAT200824
186004567
WAT200558
WAT200516
WAT200559
WAT200517
WAT200825
WAT200830
Protein
PVDF
WAT200804
WAT200805
WAT200838
WAT200560
WAT200561
WAT200828
50
0.2 m
200
1000
50
0.45 m
200
1000
Acrodisc 25 mm
Pack Size
Aqueous
GHP
NYLON
PVDF
GHP GF*
NYLON GF*
GF**
Versapor
Sterile Syringe PES
WAT097964
WAT200522
WAT200808
WAT200529
WAT097965
WAT200523
WAT200809
186004566
WAT200833
WAT200839
WAT200514
WAT200518
WAT200510
WAT200802
WAT200800
WAT200818
WAT200515
WAT200519
WAT200511
WAT200803
WAT200801
WAT200819
WAT200829
WAT200831
WAT200826
WAT058853
WAT200846
WAT200840
WAT200841
Non Polar
CR
GHP
WAT200504
WAT097964
WAT200505
WAT097965
WAT200822
186004566
WAT200500
WAT200514
WAT200501
WAT200515
WAT200820
WAT200829
Protein
PVDF
WAT200808
WAT200809
WAT200839
WAT200510
WAT200511
WAT200826
Ion Chromatography
IC
WAT200814
WAT200815
WAT200845
WAT200816
WAT200817
WAT200843
* GHP GF and Nylon GF are Glass Fiber prefilters in combination with GHP and nylon filters.
** GF Glass Fiber only 1 m
84
Filtration
www.waters.com
SIMPLY CLEAR
THE FAST ER WAY

TO CLEANER
SENSITIVIT Y IN ITS
PUREST FORM
From discovery to late phase development, Waters offers a diverse

range of sample preparation products for bioanalysis, including the
new Ostro plate for phospholipid removal.
www.waters.com/BA
2010 Waters Corporation. Waters, T he Science of W hats Possible, Ostro,

Oasis, and Sirocco are trademarks of Waters Corporation.
86
[ ACQUITY UPLC Columns and Consumables ]

ACQUITY UPLC Columns
ACQUITY UPLC Column Listing ................................................................................................................................................. 88
ACQUITY UltraPerformance LC Columns .................................................................................................................................. 90
ACQUITY UPLC Particle Technology .......................................................................................................................................... 90
NEW ACQUITY UPLC Charged Surface Hybrid (CSH) Columns ................................................................................................. 91
ACQUITY UPLC BEH Columns .................................................................................................................................................... 93
ACQUITY UPLC High Strength Silica (HSS) Columns ............................................................................................................... 95
ACQUITY UPLC Columns Selectivity Choices ............................................................................................................................ 95
VanGuard Pre-Columns............................................................................................................................................................... 96
NEW UPLC Column Kits .............................................................................................................................................................. 97
Ordering Information ................................................................................................................................................................. 99
Sample Vials Accessories for ACQUITY UPLC Systems
Screw Cap 12 x 32 mm Vials for ACQUITY UPLC Systems ..................................................................................................103
Plates for ACQUITY UPLC Systems ........................................................................................................................................104
Glass Inserts for 96-Well Plates .............................................................................................................................................104
Waters ACQUITY UPLC System Sample Manager...................................................................................................................104
Heat Seals .................................................................................................................................................................................105
Recommended Supplies for Use in Conjunction with the ACQUITY UPLC System ..............................................................................105
87
aCQUITY UPLC COLUMNS aNd CONSUMabLES
CSH C18
USP
Class No.
pH Range
(Room Temp.)
Temperature
Limits
Particle
Size
Pore
Size
Surface
Area
Carbon
Load
L1
1-11
Low pH = 80 C
High pH = 45 C
1.7 m
130
185 m2/g
15%
Selectivity Features: General purpose reversed-phase column that offers excellent pH stability and rapid mobile-phase
re-equilibration for method development. Charged Surface Hybrid (CSH) technology enables superior peak shape and increased
loading capacity for basic compounds.
Bonding: Trifunctional C18 ligand, fully end-capped, bonded to a CSH particle substrate.
O Si
O
L11
CSH
Phenyl-Hexyl
L43
O
O Si
O
O
OO Si
OO Si
O
1-8
1-12
L7
1-12
CH 3
O Si
O
Polar Group
Si 3
OOCH
OO Si
O
O
Si 3
O CH
OSi
OO
L1
Polar Group
O CH3
OO Si
OO Si
O
O
O Si
O
O Si
OCH 3
OOSi
Polar Group
Si
O CH
3
O
2-11
BEH Phenyl
O
O Si
O
O
O Si
O
BEH HILIC
O
O Si
O
Low pH = 60 C
High pH = 45 C
1.7 m
130
185 m2/g
10%
Low pH = 80 C
High pH = 60 C
1.7 m
130
185 m2/g
18%
Low pH = 60 C
High pH = 60 C
1.7 m
130
185 m2/g
13%
Polar Group
Low pH = 50 C
High pH = 45 C
1.7 m
130
185 m2/g
17%
Selectivity Features: Alternate selectivity as compared to straight chain C18, particularly with phenolic analytes.
Compatible with 100% aqueous-phase composition.
Bonding: Monofunctional embedded polar C18, fully endcapped, bonded to Ethylene Bridged Hybrid (BEH) substrate.
L11
1-12
O
O Si
O
O
CH 3
O Si
O Si
O
CH3
14%
Selectivity Features: General purpose column ideally suited for method development due to extreme pH stability and
applicability to the broadest range of compounds classes.
Bonding: Trifunctional C8, fully endcapped, bonded to Ethylene Bridged Hybrid (BEH) substrate.
CH
O Si 3
OOSi
185 m2/g
Polar Group
Si 3
O CH
O
O Si
O
BEH Shield RP18
130
applicability to the broadest range of compound classes.
O Si
O
BEH C8
1.7 m
Selectivity Features: General purpose column that provides a very high degree of analyte selectivity, especially when using
low-pH mobile phases. Charged Surface Hybrid (CSH) technology enables superior peak shape and increased loading capacity
for basic compounds.
Bonding: Trifunctional propyl fluorophenyl ligand, non-endcapped, bonded to a CSH particle substrate.
L1
BEH C18
Low pH = 80 C
High pH = 45 C
Selectivity Features: General purpose alternative selectivity ligand that provides pi-pi interactions with polyaromatic compounds,
while maintaining excellent reproducibility at pH extremes. Charged Surface Hybrid (CSH) technology enables superior peak shape
and increased loading capacity for basic compounds.
Bonding: Trifunctional C6 Phenyl ligand, fully end-capped, bonded to a CSH particle substrate.
O
O Si
O
CSH
Fluoro-Phenyl
1-11
Low pH = 80 C
High pH = 60 C
1.7 m
130
185 m2/g
15%
Selectivity Features: Excellent method development column for alternate selectivity, particularly in regard to
polyaromatic compounds. Unique level of pH stability for a Phenyl bonded phase.
Bonding: Trifunctional C6 Phenyl, fully endcapped, bonded to Ethylene Bridged Hybrid (BEH) substrate.
L3
1-9
Low pH = 45 C
High pH = 45 C
1.7 m
130
185 m2/g
unbonded
Selectivity Features: Excellent for retention of very polar, basic, water soluble analytes. Specifically designed and tested for
HILIC separations using mobile phases containing high concentrations of organic solvent.
Bonding: Unbonded Ethylene Bridged Hybrid (BEH) substrate.
O Si
O
88
ACQUITY UPLC Columns and Consumables
www.waters.com
BEH Amide
O Si
O
Linker
NH 2
(Peptide Separation
Technology)
(Protein Separation
Technology)
O
CH
O Si 3
OOSi
CH 3
O CH
Si 3
Polar Group
CH3
2-11
Low pH = 90 C
High pH = 90 C
1.7 m
130
185 m2/g
12%
Selectivity Features: Rugged HILIC stationary phase designed to separate a wide range of very polar compounds. Especially good
at separating carbohydrates (saccharides) using high concentrations of organic modifier, elevated temperature and high pH. Compatible
with all modern detectors including MS, ELSD, UV and Fluorescence.
Bonding: Trifuncional amide bonded to Ethylene Bridged Hybrid (BEH) substrate.
L1
OO Si
O Si
O
O CH
Si
O
O Si
O
O CH
Si
O
O
Low pH = 80 C
High pH = 60 C
1.7 m
130
185 m2/g
18%
Low pH = 80 C
High pH = 60 C
1.7 m
300
90 m2/g
12%
1-10
Low pH = 80 C
High pH = 50 C
1.7 m
300
90 m2/g
8%
1-12
Low pH = 80 C
High pH = 60 C
1.7 m
130
185 m2/g
18%
Selectivity Features: pH and temperature stable, small pore, C18, UPLC column for synthetic DNA and RNA. Specifically QC tested
with synthetic DNA ladder.
O Si
O
2-11
3
Polar Group
OCH 3
O
O
Si
O Si
O Si
OCH
O 3
Linker
NH 2
Polar Group
O
O
O Si
O Si
OO
O Si
O
O
OH
OH
O
Si 3
O CH
O
OOSi
O Si
CH3
O
Low pH = 90 C
High pH = 90 C
1.7 m
130
185 m2/g
12%
Selectivity Features: A HILIC UPLC column for the LC and LC/MS analysis of fluorescently labeled glycans. Specifically QC
tested with 2-AB labeled human mAb IgG derived glycans.
Bonding: Trifunctional amide bonded to Ethylene Bridged Hybrid (BEH) substrate.
L33
HSS C18
1-12
OOSi
O
OO Si
OO Si
BEH200 SEC
1-12
C4 H 9
CH3
(Glycan Separation
Technology)
Carbon
Load
Selectivity Features: pH and temperature stable, wide pore, C4, UPLC column for proteins. Specifically QC tested with protein mixture.
Bonding: Proprietary monofunctional C4 bonding to Ethylene Bridged Hybrid (BEH) substrate.
O Si
CH 3 C4 H 9
BEH Glycan
Surface
Area
L26
OO Si
OO Si
O
CH 3
(Oligonucleotide
Separation Technology)
Pore
Size
Selectivity Features: pH and temperature stable, wide pore, C18, UPLC column for peptides. Specifically QC tested with peptide map.
Polar Group
CH3
O Si
BEH C18
Particle
Size
L1
O Si
BEH300 C4
Temperature
Limits
Selectivity Features: pH and temperature stable, small pore, C18, UPLC column for peptides. Specifically QC tested with peptide map.
O Si
O
BEH300 C18
pH Range
(Room Temp.)
L1
BEH130 C18
(Peptide Separation
Technology)
USP
Class No.
1-8
Low pH = 60 C
High pH = 60 C
1.7 m
200
216 m2/g
12%
Selectivity Features: A UPLC SEC column for proteins from 10,000 to 450,000 daltons. Specifically QC tested with protein standards.
Bonding: Diol bonded to a high pore volume Ethylene Bridged Hybrid (BEH) substrate.
Polar Group
L1
O
O
O
Si
O
O Si
O Si
O
O
O
1-8
Low pH = 45 C
High pH = 45 C
1.8 m
100
230 m2/g
15%
Selectivity Features: Ultra performance C18 chemistry, increased retention, superior peak shape, resists acid hydrolysis at low pH.
Designed for UPLC separations where silica-based C18 selectivities are desired.
Bonding: High coverage trifunctional C18, fully endcapped, bonded to High Strength Silica (HSS) UPLC particle substrate.
CH 3
O Si
CH3
HSS C18 SB
O
O
Si
O Si
O
O
Polar Group
L1
www.waters.com
O
O Si
O
Low pH = 45 C
High pH = 45 C
1.8 m
100
230 m2/g
8%
Selectivity Features: Unique, non-endcapped C18 chemistry designed specifically for method development scientists.
Offers unique Selectivity for Bases (SB) when operating under low pH conditions.
Bonding: Intermediate coverage tri-functionally bonded C18, no endcapping, bonded to High Strength Silica (HSS) UPLC particle substrate.
L1
HSS T3
2-8
2-8
Low pH = 45 C
High pH = 45 C
1.8 m
100
230 m2/g
11%
Selectivity Features: Aqueous mobile-phase compatible UPLC column designed for extreme retention.
Combines polar compound retention with UPLC efficiencies and performance.
Bonding: T3 (C18) bonding and endcapping, bonded to High Strength Silica (HSS) UPLC particle substrate.
89
ACQUITY UltraPerformance LC Columns

In 2004, separation science was revolutionized with the introduction of
Ultra-Performance Liquid Chromatography [UPLC Technology]. Significant
advances in instrumentation and column technology were made to achieve
dramatic increases in resolution, speed and sensitivity in liquid chromatography.
For the first time, a holistic approach involving simultaneous innovations
in particle tec hnology and instrument design was endeavored to meet
and overcome the challenges of the analytical laboratory. T his was done
in order to make analytical scientists more successful and businesses more
profitable and productive.
For more than four decades, reducing stationary-phase particle size has been
exploited to improve chromatographic separation efficiency. Until recently,
LC tec hnology had reac hed a plateau in whic h the benefits of reducing
particle size could not be fully realized due to the negative influence of
instrument band spreading and limited pressure range.
T he ACQUIT Y UPLC System has removed those barriers, enabling
columns packed with smaller particles [1.7 1.8 m] to reac h their
theoretical performance, while precisely delivering mobile phase at
pressures up to 1030 bar [15,000 psi], thus providing a new level of
c hromatographic performance.
T he ACQUIT Y UPLC column family continues to evolve and expand, now
including 5 particle substrates [BEH 130, BEH 200, BEH 300, HSS
and CSH] and 14 chemistries which are scalable between HPLC and UPLC
particle sizes. Additionally, 6 application-directed UPLC chemistries for SEC,
amino acid analysis, proteins, peptides, oligonucleotides and glycan analysis
are available. With a number of selectivity choices for both small molecule
and biopharmaceutical applications, Waters has a UPLC column solution to
meet your application needs.
ACQUITY UPLC Particle Technology

Charged Surface Hybrid [CSH] Particles
T he 1.7 m Charged Surface Hybrid [CSH] particle is Waters 3rd generation
hybrid particle technology. Based on Waters Ethylene Bridged Hybrid [BEH]
particle technology, CSH particles incorporate a low level surface charge,
designed to improve sample loadability and peak asymmetry in low-ionicstrength mobile phases, while maintaining the mec hanical and c hemical
stability inherent in BEH particle technology.
90
T he advantages of CSH Technology include:

Superior peak shape for basic compounds
Increased loading capacity
Rapid column equilibration after changing mobile phase pH
Improved batch-to-batch reproducibility
Exceptional stability at low and high pH
CSH particle technology is also available in HPLC particle sizes [3.5 and
5 m] in the XSelect family of HPLC columns enabling seamless transfer
between HPLC and UPLC Technology platforms.
Ethylene Bridged Hybrid [BEH] Particles
T he 1.7 m Ethylene Bridged Hybrid [ BEH] particle is one of the key
enablers behind UPLC Tec hnology. It is available in 3 different pore
sizes [130, 200 and 300] and several bonded phases for reversedphase and hydrophilic interaction chromatography and is applicable from
small molecule to large biopharmaceutical analysis. Due to the intrinsic
chemical stability of hybrid particle technology, a wider usable pH range
[pH 1 -12] can be employed, enabling a versatile and robust separation
technology for method development.
BEH particle technology is also available in HPLC particle sizes [2.5, 3.5,
5 and 10 m] in the XBridge family of HPLC columns, enabling seamless
transfer between HPLC and UPLC Technology platforms.
High Strength Silica [HSS] Particles
High pore volume HPLC particles do not possess the mechanical stability
necessary to withstand the high pressures inherent of UPLC separations. T his
mechanical limitation led Waters material scientists to develop a new silica
particle designed for high mechanical stability and appropriate morphology
necessary to provide long column lifetimes and UPLC efficiencies at high
pressures. T he 1.8 m High Strength Silica [ HSS] particle is the first
and only 100% silica particle designed, tested and intended for use
in applications up to 15,000 psi [1,034 bar].
HSS particle tec hnology is also available in HPLC particle sizes [3.5
and 5 m] in the HSS family of HPLC columns, enabling seamless transfer
between HPLC and UPLC Technology platforms.
www.waters.com
ACQUITY UPLC COLUMNS AND CONSUMABLES
Particle Type
Charged-Surface Hybrid (CSH)
Ethylene-Bridged Hybrid (BEH)
High-Strength Silica (HSS)
Particle Size
1.7 m
1.7 m
1.8 m
15,000 psi (1030 bar)
15,000 psi (1030 bar)
15,000 psi (1030 bar)
Maximum Rated Pressure

Pore Diameter/Volume
130 / 0.7 mL/g
130 / 0.7 mL/g
200 / 0.7 mL/g
300 / 0.7 mL/g
100 / 0.7 mL/g
185 m2/g
185 m2/g
216 m2/g
90 m2/g
230 m2/g
C18, Phenyl-Hexyl,
Fluoro-Phenyl
C18, C8,
Shield RP18, Phenyl,
HILIC, Amide
Diol
C18, C4
C18, C18 SB, T3
C18, Phenyl-Hexyl (1-11);

Fluoro-Phenyl (1-8)
C18, C8, Phenyl (1-12);

RP18, Amide (2-11);
HILIC (1-9)
Diol (1-8)
C18 (1-12);
C4 (1-10)
C18 (1-8);
C18 SB, T3 (2-8)
Surface Area
Available Chemistries
pH Range
Waters is the only manufacturer offering FIV E UPLC-certified particles
NEW ACQUITY UPLC CSH Columns

ACQUITY UPLC CSH C18 Columns
T he ACQUIT Y UPLC CSH C 18 column overcomes the poor peak shape
encountered with most modern C18 columns in low ionic strength mobile
phases [i.e., 0.1% formic acid] due to low loading capacity.
With a controlled, low-level positive surface c harge, ACQUIT Y UPLC CSH

C18 provides industry-leading peak shape for basic compounds without
the need for ion-pairing reagents.
Trace Impurity Separation

0.10
ACQUITY UPLC CSH C18 1.7 m

0.1% formic acid
0.08
2.0 %
Imipramine
AU
0.06
0.04
Amitriptyline
1.0 %
0.5 %
0.02
0.1 %
0.00
0.00
0.20
0.40
0.60
0.80
1.00
1.20
1.40
0.10
1.60
1.80
2.00 min
Kinetex C18 1.7 m

0.1% formic acid
Core-shell
Technology
0.08
Imipramine
Trademark of Phenomenex, Inc.

0.06
AU
LC Conditions
Columns:
ACQUITY UPLC CSH C18, 2.1 x 50 mm, 1.7 m

Kinetex C18, 2.1 x 50 mm, 1.7 m
Mobile Phase A:
water
Mobile Phase B:
acetonitrile
Mobile Phase C:
2% formic acid in water
Gradient:
Time Flow 0.67

(min) (mL/min) %A %B %C
Curve

Initial 0.6
70 25 5 Initial

2.0 0.6
60 35 5 6

3.0 0.6
0 95 5 6

3.1 0.6
70 25 5 6

5.0 0.6
70 25 5 6
Injection Volume:
5 L
Sample Diluent:
water
Sample Concentration: imipramine: 0.5 mg/mL; amitriptyline: as

indicated (% of imipramine)
Column Temp.:
40 C
Strong Wash:
50/50 acetonitrile/water
Weak Wash:
Detection:
UV @ 254 nm
Sampling Rate:
20 points/sec
Filter Time Constant: 0.1 sec
Instrument:
ACQUITY UPLC H-Class with

ACQUITY UPLC PDA and ACQUITY SQ Detector
Capillary:
3.0 kV (ES+)
Cone:
30 V
Extractor:
3V
Desolvation Temp.:
350 C
Source Temperature: 120 C
Desolvation Gas:
800 L/ hr
Scan Range:
100 to 600 m/z
Scan Rate:
5000 Da/sec
0.04
2.0 %
1.0 %
0.5 %
0.02
0.1 %
0.00
0.00
Comparative separations may not be representative of all applications.
www.waters.com
0.20
0.40
0.60
0.80
1.00
1.20
1.40
1.60
1.80
2.00 min
The increased and improved loadability possible with CSH Technology permits the separation, identification and quantitation of closely
eluting impurities or degradants. In this example, a high concentration (0.5 mg/mL) of imipramine was repeatedly injected while the
concentration of amitriptyline was increased with each injection.
91
ACQUITY UPLC CSH Fluoro-Phenyl Columns
ACQUITY UPLC CSH Phenyl-Hexyl Columns
One of the challenges in designing stationary phases with large selectivity

differences is maintaining an acceptable level of batc h-to-batc h reproducibility. Many commercially available columns with unique selectivities suffer from significant variability, particularly phases bonded with
a pentafluorophenyl [ PF P] ligand.
T he ACQUIT Y UPLC CSH Phenyl-Hexyl column provides complementary

selectivity compared to a straight c hain alkyl C18 column, particularly
for aromatic species.
T he 1.7 m ACQUIT Y UPLC CSH Fluoro-Phenyl column overcomes this

limitation due to the CSH Tec hnology bonding process. T his process not
only yields exceptional batc h-to-batc h reproducibility, but sustained
selectivity across particle sizes wit h its HPLC analog, XSelect CSH
Fluoro-Phenyl [available in 3.5 and 5 m particle sizes].
Reproducibility and Scalability of ACQUITY UPLC CSH Fluoro-Phenyl
[1.7 m] and XSelect CSH Fluoro-Phenyl [3.5, 5 m]
Columns:
Gradient:
Temperature:
Injection Volume:
Detection:
Flow Rate:
System:
ACQUITY UPLC CSH Fluoro-Phenyl 1.7 m

XSelect CSH Fluoro-Phenyl 3.5, 5 m
A: 15.4 mM ammonium formate, pH 3
B: acetonitrile
5 to 90% B linear in 5 minutes
30 C
5 L
254 nm
0.5 mL/min
ACQUITY UPLC
Compounds:
1. T hiourea
2. Resorcinol
3. Metoprolol
4. 3-Nitrophenol
5. 2-Chlorobenzoic acid
6. Amitriptyline
7. Diethylphthalate
8. Fenoprofen
9. Dipropylphthalate
10. Pyrenesulfonic acid
With a controlled, low-level positive surface c harge, the ACQUIT Y UPLC

CSH P henyl-Hexyl column provides industry-leading peak s hape for
basic analytes in low pH mobile phases.
Fast Separation of Basic Drugs on an

ACQUITY UPLC CSH Phenyl-Hexyl Column
Column:
Mobile Phase A:
Mobile Phase B:
Gradient:
Injection Volume:
Column Temperature:
Detection:
System:
ACQUITY UPLC CSH Phenyl-Hexyl,

2.1 x 50 mm, 1.7 m
10 mM NH4COOH, pH 3
MeCN; 5 to 55% B
linear in 3 minutes
4 L
30 C
UV @ 260 nm
ACQUITY UPLC with
ACQUITY UPLC PDA
Compounds:
1.
2.
3.
4.
5.
6.
7.
8.
9.
Aminopyrazine
Pindolol
Quinine
Quinine impurity
Labetalol
Diltiazem impurity
Diltiazem
Verapamil
Amitriptyline
ACQUITY and XSelect CSH Fluoro-Phenyl
0.02
7
5 m
0.16
9
3
0.12
8
0.08
1
3.5 m
0.04
6
4
0.00
0.00
0.50
1.00
1.50
2.00
2.50
3.00 min
1.7 m
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0 min
Gradient separations on 2.1 x 50 mm columns containing nine different batches of

CSH Technology Fluoro-Phenyl representing three (1.7, 3.5, and 5 m) particle sizes.
92
www.waters.com

ACQUITY UPLC BEH Columns
ACQUITY UPLC BEH C18 and C8 Columns
Providing unprecedented levels of peak asymmetry, efficiency and chemical
stability, t he 1.7 m ACQUIT Y U P LC B EH C 18 and C 8 columns are
versatile, high-performance separation columns suitable for a diverse
range of analytes. Wit h t he ability to operate between pH 1 -12,
these trifunctionally bonded alkyl columns can employ the power of pH
to impact the retention, selectivity and sensitivity of ionizable compounds
while delivering exceptional low and high pH stability.
Separation of Explosives on ACQUITY UPLC BEH Columns

Column:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Isocratic Mobile
Phase Composition:
Injection Volume:
Sample Conc.:
Temperature:
Detection:
Instrument:
ACQUITY UPLC BEH, 2.1 x 100 mm, 1.7 m

H2O
MeOH
0.5 mL/min
Compounds
1. HMX
2. RDX
3. 1,3,5-TNB
4. 1,3-DNB
5. NB
6. Tetryl
7. TNT
8. 2-Am-4,6 DNT
9. 4-Am-2,6 DNT
10. 2,4 DNT
11. 2,6-DNT
12. 2-NT
13. 4-NT
14. 3-NT
72% A; 28% B
5 L
10 g/mL
50 C
UV @ 254 nm
ACQUITY UPLC with PDA detector
ACQUITY UPLC BEH Shield RP18 Columns

T he ACQUITY UPLC BEH Shield RP18 Columns incorporate a hydrophilic
carbamate group within a C18 chain to produce exceptional peak shape for
basic compounds as well as alternate selectivity compared to straight chain
alkyl columns.
3
1
2
5
ACQUITY UPLC BEH Phenyl Columns
10
8
11
10
8
6 9
11
BEH C8
12 13 14
3
1
8
9
13
10
6
11
10
11
12 13
14
T he ACQUITY UPLC BEH HILIC column utilizes an unbonded BEH particle to

improve the retention of very polar species as well as provide an orthogonal
separation mode for mixtures of polar and ionizable compounds.
T he ACQUITY UPLC BEH HILIC column is optimized and tested to produce
efficient, reproducible separations under UPLC HILIC conditions and are
designed to overcome a major weakness of HILIC stationary phases:
c hemical stability. T he rugged BEH particles wide usable pH range
overcomes this chemical instability and results in long column lifetimes.
BEH Shield RP18
14
12
4
2
ACQUITY UPLC BEH HILIC Columns
BEH C18
12 13 14
1 2
T he ACQUIT Y UPLC BEH Phenyl Columns incorporate a trifunctionally

bonded phenyl-hexyl ligand to provide industry-leading pH stability,
batch-to-batch reproducibility and exceptional peak shape compared to other
commercially available phenyl columns. T he ACQUITY UPLC BEH Phenyl
column is designed to provide complementary selectivity to straight
c hain alkyl columns, particularly for analytes containing aromatic rings.
BEH Phenyl
7
10
12
14
min
Separation of Very Polar Bases Using

ACQUITY UPLC BEH HILIC Columns
Column:
Part Number:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
Injection Volume:
Sample Conc.:
Sample Diluent:
Temperature:
Detection:
Instrument:
ACQUITY UPLC BEH HILIC, 2.1 x 100 mm, 1.7 m

186003461
10 mM NH4COOH, 0.2% HCOOH in 50:50 ACN:H2O
10 mM NH4COOH, 0.2% HCOOH in 90:10 ACN:H2O
0.788 mL/min
Time
Profile
(min)
%A
%B
1. Morphine
0.0
0.1
99.9
HO
0.37
0.1
99.9
1.46
99.9
0.1
1.50
0.1
99.9
O
H
N
2.00
0.1
99.9
2.1 L
HO
125 g/mL
75:25 ACN:MeOH with
0.2% HCOOH
1
30 C
2. Morphine 3-fl-D-Glucronide
C H3
N
C H3
OH
O O
HO
OH
HO
OH
UV @ 280 nm
ACQUITY UPLC with TUV Detector
V0
0.0
www.waters.com
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0 min
93
ACQUITY UPLC BEH Amide Columns
Carbohydrate Analysis
Hydrophilic Interaction Chromatography
One of the most abundant and diverse classes of compounds analyzed

by HILIC are carbohydrates [e.g., monosacc harides, disacc harides, oligosacc harides and polysacc harides].
Since 2003, Waters has developed innovative stationary phases for

Hydrophilic Interaction Chromatography [HILIC] to overcome the challenge
of retaining and separating extremely polar compounds. Based on Waters
novel ethylene bridged hybrid [BEH] particle tec hnology, ACQUIT Y UPLC
BEH Amide columns utilize a c hemically stable, trifunctionally-bonded
amide phase, enabling a new dimension in stability and versatility for
HILIC separations.
Designed to retain polar analytes and metabolites that are too polar to
retain by reversed-phase [RP] chromatography, ACQUITY UPLC BEH Amide
columns facilitate the use of a wide range of mobile phase pH [2 11] to
facilitate the exceptional retention of polar analytes spanning a wide range
in polarity, structural moiety and pKa.
In addition to enhanced retention of polar compounds, ACQUIT Y UPLC
BEH Amide columns provide increased mass spectrometry response, direct
compatibility with sample preparation eluates [ PPT, LLE and SPE] as well as
orthogonal selectivity compared to reversed-phase materials, making HILIC
an attractive alternative separation technique.
Separation of Water Soluble Vitamins

Column:
Part Number:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
T he 1.7 m particle size enables high resolution, high speed analysis

of carbohydrates in complex sample matrices while maintaining or
improving c hromatographic resolution.
Increased c hemical stability enables the use of high pH and high

temperature to collapse reducing sugar anomers, shorten analysis
times and improve MS detection.
BEH particle tec hnology, in combination with a trifunctionallybonded amide phase, provides exceptional column lifetime, thus
improving assay robustness.
Unlike amine-based columns used for carbohydrate analysis, the

ACQUIT Y UPLC BEH Amide column is not susceptible to Sc hiff-base
formation, thus improving quantitation accuracy.
High pH mobile-phase compatibility facilitates MS detection without

the need for derivatization, post column addition or complexing with
a metal cation, thus dramatically simplifying sample pre-treatment
before LC/MS analysis and improving assay sensitivity.
UPLC/MS Analysis of Saccharides
ACQUITY UPLC BEH Amide, 2.1 x 50 mm, 1.7 m

186004800
50/50 ACN/H2O with 10 mM CH3COONH4 and 0.04% NH4OH, pH 9.0
90/10 ACN/H2O with 10 mM CH3COONH4 and 0.04% NH4OH, pH 9.0
0.5 mL/min
Time
Profile
(min)
%A
%B
0.00
0.1
99.9
3.50
70.0
30.0
3.51
0.1
99.9
7.50
0.1
99.9
5.0 L (PLNO)
75/25 ACN/MeOH with 0.2% HCOOH
30 C
ACN/H2O 95/5
UV @ 265 nm
20 points/sec
Normal
Waters ACQUITY UPLC with
ACQUITY UPLC PDA Detector
Compounds:
6
1. Nicotinamide
2. Pyridoxal
3. Riboflavin
4. Nicotinic acid
5. T hiamine
6. Ascorbic Acid
7. B12
5
8. Folic Acid
Injection Volume:
Sample Diluent:
Column Temp.:
Weak Needle Wash:
Detection:
Sampling Rate:
Filter Time Constant:
Instrument:
T he ACQUIT Y UPLC BEH Amide column is an exceptional tool for the

analysis of carbohydrates, providing the following benefits:
Column:
Part Number:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
Injection Volume:
Sample Diluent:
Column Temp.:
Needle Wash:
Instrument:
25 g/mL
50 g/mL
50 g/mL
50 g/mL
50 g/mL
25 g/mL
50 g/mL
25 g/mL
Ionization Mode:
Capillary:
Cone Voltage:
Source Temp.:
Desolv. Temp.:
Desolv. Gas Flow:
SIR m/z:
Dwell Time:
ACQUITY UPLC BEH Amide, 2.1 x 50 mm, 1.7 m

186004800
30/70 MeCN/H2O with 0.10% NH4OH
0.17 mL/min
Time
Profile
(min)
%A
%B
Initial
0
100
5.00
60
40
5.01
0
100
0.7 L (PLNO)
3
50/50 MeCN/H2O
35 C
ACN/H2O 75/25
Waters ACQUITY UPLC with
ACQUITY UPLC TQD
ES2.8 kV
25 V
120 C
350 C
500 L/Hr
179.2 (fructose, glucose);
341.3 (sucrose, maltose);
503.4, 665.5,
827.6, 989.7, 115
(maltooligosaccharides
[n=1 to 5])
5
0.08 s
4
Compounds:
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
5. Maltotriose
6. Maltotetraose
7. Maltopentaose
8. Maltohexahose
9. Maltoheptaose
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
2
7
7
3
min
94
7 min
www.waters.com

ACQUITY UPLC HSS Columns
ACQUIT Y UPLC HSS columns are available in three bonded phases:
C18, C18 SB and T3.
ACQUITY UPLC HSS C18
T he ACQUITY UPLC HSS C18 chemistry is an ultra-performance, generalpurpose C18 bonded phase that provides superior peak shape for bases,
increased retention (vs. ACQUITY UPLC BEH C18 columns), and extremely
long column lifetimes at low pH. T he selectivities and retention observed
with HSS C18 columns will resemble that of most modern, fully endcapped
silica-based C18 HPLC columns.
ACQUITY UPLC HSS C18 SB
T he ACQUIT Y UPLC HSS C 18 SB (Selectivity for Bases) c hemistry is
an unendcapped C18 bonded phase that was designed specifically to be
different in terms of selectivity. T he HSS C18 SB chemistry is optimized
for low pH method development applications and provides alternate
selectivities, especially for basic compounds, as compared to most modern,
fully endcapped C18 chemistries.
Caffeic Acid Derivatives Separations on ACQUITY UPLC HSS Columns

Column:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
ACQUITY UPLC HSS, 2.1 x 50 mm, 1.8 m

0.1% CF3COOH in H2O
0.08% CF3COOH in ACN
0.5 mL/min
Time
Profile
(min)
%A
%B
Curve
0.0
92
8
6
0.1
92
8
6
4.45
50
50
7
4.86
10
90
6
5.0
92
8
6
6.0
92
8
6
1.0 L
50:50 H2O: MeOH
with 0.05% CF3COOH
100 g/mL
40 C
UV @ 330 nm
ACQUITY UPLC with
ACQUITY UPLC TUV
Injection Volume:
Sample Diluent:
Sample Conc.:
Temperature:
Detection:
Instrument:
HSS C18
ACQUITY UPLC HSS T3

T he ACQUIT Y UPLC HSS T3 columns are designed to solve a common
problem facing separations scientists: retaining small, water soluble, polar
organic molecules. T he HSS T3 c hemistry is an aqueous mobile phase
compatible C18 bonded phase designed to retain and separate polar organic
compounds, much like Atlantis T3 HPLC columns.
Compounds
1. Caftaric acid
2. Chlorogenic acid
3. Cynarin
4. Echinacoside
5. Cichoric acid
5
4
HSS C18 SB
HSS T3
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0 min
ACQUITY UPLC Columns Selectivity Choices
By combining ultra-efficient particle tec hnology, low dispersion instrumentation and different column ligands, chromatographers can efficiently
and effectively develop robust methods faster than ever before.
Low pH UPLC Selectivities

0.0
Selectivity
ln (amintriptyline/toluene) pH 3.0
UPLC Tec hnology improves c hromatographic resolution by maximizing

system efficiency. T his is achieved by minimizing the dispersion contribution
of the LC system in combination with the use of sub-2 m column chemistries
run at their optimal linear velocities. In addition to utilizing small, sub-2 m
particles, resolution can also be improved by utilizing different stationaryphase ligands to alter selectivity.
HSS C 18 SB
-0.2
BEH300 C4
BEH Phenyl
-0.4
-0.6
BEH C8
-0.8
BEH C18
BEH300 C18
-1.0
CSH Phenyl-Hexyl
-1.2
CSH Fluoro-Phenyl
-1.4
BEH
Shield RP18
HSS T3
HSS C 18
CSH C18
-1.6
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
Hydrophobicity ln k (toluene)
35% MeCN/65% 15.4 mM HCOONH4, pH 3.0
Waters offers the most comprehensive and advanced offering

of UPLC particle substrates and column technology.
www.waters.com
95

VanGuard Pre-Columns
Separation scientists working in demanding application areas (suc h as
bioanalysis, food/beverage, natural products, environmental, and industrial
chemicals) analyze complex, unpredictable and challenging samples on a
routine basis. T hese types of samples can have a negative impact on column
lifetimes when appropriate sample preparation/cleanup procedures are not
implemented. VanGuard pre-columns are designed for these types of
application areas where c hemical and/or particulate contamination can
shorten the lifetime of a UPLC column.
VanGuard pre-columns are t he result of over two years of product
development and are t he first guard column devices designed for
routine use at pressures up to 15000 psi (1030 bar) in applications
run on t he ACQUIT Y U PLC System. VanGuard pre-columns feature a
2.1 mm i.d. x 5 mm length, ultra-low volume design whic h efficiently
protects UPLC column performance. T his patent-pending design does not
compromise the UPLC holistic design approach to higher efficiency, greater
resolution and increased throughput since the same ACQUITY UPLC column
stationary phases and column frits are used in VanGuard pre-columns. Since
the VanGuard pre-column connects directly to the inlet of the ACQUIT Y
UPLC column, extra-column volumes are minimized and mobile phase leaks
due to additional connections are all but eliminated.
Key Features and Benefits of VanGuard Pre-Columns
FEATURE
BENEFIT
First pre-column for

UPLC applications
Guaranteed compatibility with

pressures up to 15000 psi
Patent pending, ultra-low

volume design
Minimal chromatography effects
Manufactured using UPLC column

hardware, particles, and chemistries
Superior UPLC column

protection and performance
Connects directly to UPLC column
Leaks and connection

voids are eliminated
Minimal Chromatographic Effects With VanGuard Pre-Columns

VanGuard pre-columns are uniquely designed to protect and prolong ACQUITY UPLC column
performance while contributing minimal chromatographic effects.
Column:
Part Number:
Pre-column:
Part Number:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
Injection Volume:
Temperature:
Detection:
Sampling Rate:
Time Constant:
Instrument:
ACQUITY SQ Detector
ES+
Capillary:
Cone:
Source Temp:
Desolvation Temp:
Cone Gas Flow:
SIR:
MS Interscan Delay:
Dwell:
ACQUITY UPLC BEH C18, 2.1 x 50 mm, 1.7 m

186002350
VanGuard pre-column, BEH C18, 2.1 x 5 mm, 1.7 m
186003975
0.2% NH4OH in H2O
0.2% NH4OH in ACN
0.8 mL/min
Time
Profile
(min)
%A
%B
0.0
95
5
2.0
5
95
2.5
5
95
2.6
95
5
3.0
95
5
2 L
40 C
UV @ 254 nm
40 pts/sec
0.05
ACQUITY UPLC with ACQUITY UPLC
TUV detector and ACQUITY SQ detector
3.5 kV
35 V
150 C
500 C
50 L/Hr
850 L/Hr
373.2 m/z, 415.2 m/z
0.005 sec
0.005 sec
Protein Precipitation Procedure

OCH3
S
Desacetyl Diltiazem
SIR m/z 373.2
Without VanGuard pre-column
With VanGuard pre-column
Add 600 L of acetonitrile to the centrifuge

tube containing the spiked plasma
Centrifuge for 10 minutes at 13000 RPM
Take 650 L of supernatent and evaporate

to dryness with nitrogen
OCH3
N
N
0.8
1.0
Reconstitute with 400 L of a 50:50 MeOH:H2O

solution (results in a 203.125 ng/mL conc.)
Diltiazem
SIR m/z 415.2
Without VanGuard pre-column
With VanGuard pre-column
0.6
96
Take 200 L of spiked plasma and

add to 1.5 mL centrifuge tube
OH
Spike plasma with 500 ng/mL of diltiazem

and desacetyl diltiazem
1.2
1.4
1.6
1.8
O
O O
2.0 min
www.waters.com

NEW UPLC Column Kits
More and more organizations have realized the benefits of improved
productivity, higher data quality, and lower cost per sample as well as
faster time-to-market, inherent in assays that utilize UPLC Tec hnology.
T he ACQUIT Y UPLC H-Class System is a result of this paradigm shift,
enabling the efficacy of a method to be preserved as it is transferred
between LC platforms.
UPLC Separation on ACQUITY UPLC H-Class System

Clozapine
Transfer Between LC Technology Platforms With Ease
2.1 x 50 mm, 1.7 m
In addition to the proper geometric scaling of all method parameters,

the successful transfer of an analytical method requires the preservation
of c hromatographic column selectivity and resolving power, regardless
of particle size.
3.2
4.0
4.8
5.6
min
HPLC Separation on Alliance HPLC System

Clozapine
Waters industry-leading manufacturing processes not only ensures

unprecedented batc h-to-batc h reproducibility, but also sustained
selectivity between HPLC and UPLC particle sizes. New Method Transfer
kits leverage this capability, enabling the successful transfer of methods
from one LC platform to another.
ACQUITY UPLC BEH Shield RP18
XBridge Shield RP18

4.6 x 100 mm, 3.5 m
1.7 m
1.8 m
1.7 m
12
3.5, 5.0 m
3.5, 5.0 m
20
24 min
HPLC Separation on ACQUITY UPLC H-Class System

Clozapine
2.5, 3.5, 5.0, 10 m
16
XBridge Shield RP18

4.6 x 100 mm, 3.5 m
12
16
20
24 min
A separation of clozapine and its related compounds is used to demonstrate the exceptional scalability between
different size chromatographic particles and instrument platforms to facilitate a successful method transfer.
Retention Time Relative to Clozapine
www.waters.com
Separation Mode
Instrument
Impurity D
Impurity C
Impurity A
Impurity B
UPLC
ACQUITY UPLC H-Class System
0.867
0.890
0.939
1.500
HPLC
Alliance 2695 System
0.865
0.895
0.950
1.513
HPLC
ACQUITY UPLC H-Class System
0.867
0.898
0.951
1.507
97
ACQUITY UPLC CoLUmns And ConsUmAbLes
Take the Guesswork Out of Method Transfer

New Method Transfer kits* are designed to preserve the integrity of
a separation as it is transferred between UPLC and HPLC platforms.
Based on the concept of maintaining column length [ L] to particle size
[dp] ratio [ L/dp], these kits provide an ACQUIT Y UPLC column with an
HPLC column of equivalent selectivity and resolving power. Using the
ACQUIT Y UPLC columns calculator, methods can be fully transferred
from HPLC to UPLC or from UPLC to HPLC.
Method Transfer Kit
* Eac h kit contains one UPLC column and one HPLC column. T he ACQUIT Y UPLC
columns calculator can be downloaded from the ACQUITY UPLC online community
at: www.waters.com/myuplc
Develop Methods With Ease

With a seemingly endless number of method parameters to try, developing
a new c hromatographic met hod can be an overw helming, and timeconsuming experience. Essential to any method development strategy
is finding a suitable column that delivers the desired separation while
resulting in a robust and reliable separation.
Waters Method Development kits consist of several UPLC columns,

encompassing a broad range in selectivity, to accommodate your
method development approac h and enable methods to be developed
efficiently and effectively.
Package Name
Chemistries
Method Development Strategy
Maximum Selectivity UPLC

Method Development kit
CSH C18, CSH Phenyl-Hexyl,

CSH Fluoro-Phenyl, HSS C18 SB
The widest selectivity offering for method development at low and high pH.
Best choice for low ionic strength additives (i.e., formic acid)
High & Low pH, Widest Selectivities

UPLC Columns kit
BEH C18, BEH C8, BEH Shield RP18,

BEH Phenyl
Maximize separation selectivity by exploring low and high mobile-phase pH
UPLC Method Development kit
BEH C18, BEH Shield RP18,

BEH Phenyl, HSS T3
Maximize separation selectivity by exploring low and high mobile-phase pH

(BEH columns) and accommodate for the retention of polar compounds (HSS T3)
L1 UPLC Columns kit

HSS C18, HSS T3
C18 columns that differ in silanol activity and hydrophobicity within the US
Pharmacopeia L1 classification
Mass Spec UPLC Columns kit
BEH C18, HSS C18, HSS T3,

HSS C18 SB
Straight-chain alkyl C18 columns that differ in silanol activity, shape, selectivity and
hydrophobicity, and exhibit no MS bleed
Low pH, Widest Selectivities

UPLC Columns kit
BEH Shield RP18, BEH Phenyl,

HSS C18, HSS C18 SB
A diverse grouping of column selectivities for the development of a reversed-phase

method in low pH mobile phases
Maximum Selectivity RP and HILIC

CSH C18, CSH Phenyl-Hexyl,

CSH Fluoro-Phenyl, BEH Amide
Offers the widest separation selectivity by combining CSH reversed-phase and HILIC
stationary phases to retain analytes encompassing a broad range of polarity
UPLC RP and HILIC Method Development kit

BEH Amide, HSS C18 SB
A novel approach that maximizes separation selectivity by combining distinct RP and

HILIC stationary phases to retain analytes encompassing a broad range of polarity
UPLC HILIC Method Development kit
BEH Amide, BEH HILIC
Effortlessly develop HILIC methods at low pH (bases) or high pH (acids) for polar
and/or ionizable compounds
Ensure Method Robustness

T he reproducibility of the chromatographic column has a critical impact on
the long-term reliability and robustness of an analytical method, yet its
reproducibility lies completely outside the users control.
With exceptional batc h-to-batc h and column-to-column reproducibility,
Waters well-established particle and column manufacturing process control
98
provides confidence in the long-term reliability of your analytical method.

ACQUITY UPLC Method Validation kits include three batches of chromatographic media [derived from different base particles] to judge the quality,
reliability, and consistency of your chromatographic method.
www.waters.com

NEW ACQUITY UPLC CSH Columns
Chemistry
Particle
Size
Dimensions
Part No.
1 pack
Part No.
3 pack
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH C18
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Fluoro-Phenyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
CSH Phenyl-Hexyl
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
186005292
186005293
186005294
186005295
186005296
186005620
186005297
186005298
186005299
186005300
186005623
186005301
186005302
186005349
186005347
186005348
186005350
186005351
186005622
186005352
186005353
186005354
186005355
186005625
186005356
186005357
186005404
186005402
186005403
186005405
186005406
186005621
186005407
186005408
186005409
186005410
186005624
186005411
186005412
176002136
176002137
176002138
176002139
176002140
176002141
176002142
176002143
176002144
176002145
176002146
176002150
176002148
176002149
176002151
176002152
176002153
176002154
176002155
176002156
176002157
176002158
176002161
176002159
176002160
176002162
176002163
176002164
176002165
176002166
176002167
176002168
176002169
Waters Carbohydrate Method

Selection Tool
T he new online carbohydrate
separation method selector
tool will recommend the best
method conditions in just
four easy steps.
Chemistry
Particle
Size
Dimensions
Part No.
1 pack
Part No.
3 pack
BEH C18
BEH C18
BEH C18
BEH C18
BEH C18
NEW BEH C18
BEH C18
BEH C18
BEH C18
BEH C18
NEW BEH C18
BEH C18
BEH C18
BEH Shield RP18
BEH Shield RP18
BEH Shield RP18
BEH Shield RP18
BEH Shield RP18
NEW BEH Shield RP18
BEH Shield RP18
BEH Shield RP18
BEH Shield RP18
BEH Shield RP18
NEW BEH Shield RP18
BEH Shield RP18
BEH Shield RP18
BEH C8
BEH C8
BEH C8
BEH C8
BEH C8
NEW BEH C8
BEH C8
BEH C8
BEH C8
BEH C8
NEW BEH C8
BEH C8
BEH C8
BEH Phenyl
BEH Phenyl
BEH Phenyl
BEH Phenyl
BEH Phenyl
NEW BEH Phenyl
BEH Phenyl
BEH Phenyl
BEH Phenyl
BEH Phenyl
NEW BEH Phenyl
BEH Phenyl
BEH Phenyl
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
186002344
186002346
186002347
186002349
186002350
186005604
186002352
186002353
186004659
186004660
186005609
186004661
186004690
186002851
186002852
186003373
186003909
186002853
186005605
186002854
186003376
186004667
186004668
186005610
186004669
186004670
186002875
186002876
186003374
186003910
186002877
186005606
186002878
186003377
186004663
186004664
186005611
186004665
186004666
186002882
186002883
186003375
186003911
186002884
186005607
186002885
186003378
186004671
186004672
186005612
186004673
186004674
176000861
176000862
176001044
176001304
176000863
176000864
176001048
176001794
176001795
176001796
176001797
176000874
176000875
176001045
176001309
176000876
176000877
176001049
176001802
176001803
176001804
176001805
176000882
176000883
176001046
176001310
176000884
176000885
176001050
176001798
176001799
176001800
176001801
176000905
176000906
176001047
176001311
176000907
176000908
176001051
176001806
176001807
176001808
176001809
For more information please visit:
www.waters.com/amidemethod
www.waters.com
99

ACQUITY UPLC HSS Columns
Chemistry
Particle
Size
Dimensions
Part No.
1 pack
Part No.
3 pack
BEH HILIC
BEH HILIC
BEH HILIC
BEH HILIC
NEW BEH HILIC
BEH HILIC
BEH HILIC
BEH HILIC
NEW BEH HILIC
BEH HILIC
BEH HILIC
BEH Amide
BEH Amide
BEH Amide
BEH Amide
BEH Amide
NEW BEH Amide
BEH Amide
BEH Amide
BEH Amide
BEH Amide
NEW BEH Amide
BEH Amide
BEH Amide
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
186003457
186003458
186003459
186003460
186005608
186003461
186003462
186004675
186005613
186004676
186004677
186004848
186004849
186004850
186004839
186004800
186005657
186004801
186004802
186004803
186004804
186005658
186004805
186004806
176001089
176001090
176001091
176001092
176001093
176001094
176001810
176001811
176001812
176001914
176001915
176001916
176001906
176001907
176001908
176001909
176001910
176001911
176001912
176001913

T he Beginners Guide to UPLC, is a 52-page book designed to provide
new, existing and potential UPLC users the ability to understand how
UPLC technology works, how to be successful with it, and how it can
provide impactful results within organizations.
Chemistry
Particle
Size
Dimensions
Part No.
1 pack
Part No.
3 pack
HSS T3
HSS T3
HSS T3
HSS T3
HSS T3
NEW HSS T3
HSS T3
HSS T3
HSS T3
HSS T3
NEW HSS T3
HSS T3
HSS T3
HSS C18
HSS C18
HSS C18
HSS C18
HSS C18
NEW HSS C18
HSS C18
HSS C18
HSS C18
HSS C18
NEW HSS C18
HSS C18
HSS C18
HSS C18 SB
HSS C18 SB
HSS C18 SB
HSS C18 SB
HSS C18 SB
NEW HSS C18 SB
HSS C18 SB
HSS C18 SB
HSS C18 SB
HSS C18 SB
NEW HSS C18 SB
HSS C18 SB
HSS C18 SB
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
186003535
186003536
186003537
186003944
186003538
186005614
186003539
186003540
186004678
186004679
186005617
186004680
186004681
186003529
186003530
186003531
186003987
186003532
186005615
186003533
186003534
186004682
186004683
186005618
186004684
186004685
186004114
186004115
186004116
186004117
186004118
186005616
186004119
186004120
186004686
186004687
186005619
186004826
186004689
176001127
176001129
176001130
176001375
176001131
176001132
176001133
176001813
176001814
176001815
176001816
176001121
176001122
176001123
176001398
176001124
176001125
176001126
176001817
176001818
176001819
176001820
176001556
176001557
176001558
176001559
176001560
176001561
176001562
176001821
176001822
176001823
176001824
Quest for Ultra Performance

in Liquid Chromatography
T he Quest for Ultra Performance in Liquid Chromatography, a
54-page book, presents a brief review of the origin and history of LC,
showing how early the concepts of ultra performance were recognized
and how many decades it took to reduce them to practice.

T his 72 page tec hnology primer is designed to provide the reader
with the basic insight of how to be successful with hydrophilic
interaction c hromatography by understanding how the tec hnique
works, t he parameters t hat impact retention and selectivity,
as well as the practical considerations necessary to successfully
implement HILIC within a c hromatographic strategy.
100
Description
Part No.
715002099
715002098
715002531
www.waters.com

ACQUITY UPLC Method Development Kits
Package Name
NEW Maximum Selectivity
UPLC Columns kit
UPLC Columns kit
UPLC Columns kit
UPLC Columns kit
L1 UPLC Columns kit
L1 UPLC Columns kit
L1 UPLC Columns kit
L1 UPLC Columns kit
Low pH, Widest Selectivities UPLC Columns kit
NEW Maximum Selectivity RP and
HILIC Method Development kit
www.waters.com
Qty/Pk
Chemistries
Particle Size(s)
Dimensions
Part No.
4/pk
CSH C18, CSH Phenyl-Hexyl, CSH Fluoro-Phenyl, HSS C18 SB
CSH: 1.7 m; HSS: 1.8 m
2.1 x 50 mm
176002123
4/pk
CSH: 1.7 m; HSS: 1.8 m
2.1 x 100 mm
176002124
4/pk
CSH: 1.7 m; HSS: 1.8 m
3.0 x 50 mm
176002125
4/pk
CSH: 1.7 m; HSS: 1.8 m
3.0 x 100 mm
176002126
4/pk
BEH C18, BEH C8, BEH Shield RP18, BEH Phenyl
BEH: 1.7 m
2.1 x 50 mm
176001042
4/pk
BEH: 1.7 m
2.1 x 100 mm
176001043
4/pk
BEH: 1.7 m
3.0 x 50 mm
176001881
4/pk
BEH: 1.7 m
3.0 x 100 mm
176001882
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
4/pk
BEH C18, BEH Shield RP18, BEH Phenyl, HSS T3

BEH C18, BEH Shield RP18, HSS C18, HSS T3
BEH C18, HSS C18, HSS C18 SB, HSS T3
BEH Shield RP18, BEH Phenyl, HSS C18, HSS C18 SB
BEH: 1.7 m; HSS: 1.8 m

BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
2.1 x 50 mm
2.1 x 100 mm
3.0 x 50 mm
3.0 x 100 mm
2.1 x 50 mm
2.1 x 100 mm
3.0 x 50 mm
3.0 x 100 mm
2.1 x 50 mm
2.1 x 100 mm
3.0 x 50 mm
3.0 x 100 mm
2.1 x 50 mm
2.1 x 100 mm
3.0 x 50 mm
3.0 x 100 mm
176001603
176001604
176001883
176001884
176001605
176001606
176001885
176001886
176001607
176001608
176001887
176001888
176001609
176001610
176001889
176001890
4/pk
CSH C18, CSH Phenyl-Hexyl, CSH Fluoro-Phenyl, BEH Amide
CSH: 1.7 m; BEH: 1.7 m
2.1 x 50 mm
176002127
4/pk
CSH: 1.7 m; BEH: 1.7 m
2.1 x 100 mm
176002128
4/pk
CSH: 1.7 m; BEH: 1.7 m
3.0 x 50 mm
176002129
4/pk
CSH: 1.7 m; BEH: 1.7 m
3.0 x 100 mm
176002130
4/pk
4/pk
4/pk
4/pk
2/pk
2/pk
2/pk
2/pk
BEH C18, BEH Shield RP18, BEH Amide, HSS C18 SB

BEH: 1.7 m; HSS: 1.8 m

BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH: 1.7 m; HSS: 1.8 m
BEH 1.7 m
BEH 1.7 m
BEH 1.7 m
BEH 1.7 m
2.1 x 50 mm
2.1 x 100 mm
3.0 x 50 mm
3.0 x 100 mm
2.1 x 50 mm
2.1 x 100 mm
3.0 x 50 mm
3.0 x 100 mm
176001959
176001960
176001961
176001962
176001963
176001964
176001965
176001966
101

ACQUITY UPLC Method Validation Kits
ACQUITY UPLC Method Transfer Kits*

UPLC Column
2.1 mm I.D.
HPLC Column
4.6 mm I.D.
Part No.
50 mm, 1.7 m
50 mm, 1.7 m
150 mm, 5 m
150 mm, 5 m
186005529
186005530
50 mm, 1.7 m
150 mm, 5 m
186005531
50 mm, 1.7 m
50 mm, 1.7 m
50 mm, 1.7 m
50 mm, 1.8 m
50 mm, 1.8 m
50 mm, 1.8 m
50 mm, 1.7 m
150 mm, 5 m
150 mm, 5 m
150 mm, 5 m
150 mm, 5 m
150 mm, 5 m
150 mm, 5 m
100 mm, 3.5 m
186004958
186004959
186004960
186004961
186004962
186004963
186005532
50 mm, 1.7 m
100 mm, 3.5 m
186005533
50 mm, 1.7 m
100 mm, 3.5 m
186005534
50 mm, 1.7 m
50 mm, 1.7 m
50 mm, 1.7 m
50 mm, 1.7 m
50 mm, 1.8 m
50 mm, 1.8 m
50 mm, 1.8 m
100 mm, 3.5 m

100 mm, 3.5 m
100 mm, 3.5 m
100 mm, 3.5 m
100 mm, 3.5 m
100 mm, 3.5 m
100 mm, 3.5 m
186004964
186004965
186004966
186004967
186004968
186004969
186004970
100 mm, 1.7 m 150 mm, 3.5 m
186005535
100 mm, 1.7 m 150 mm, 3.5 m
186005536
100 mm, 1.7 m 150 mm, 3.5 m
186005537
100 mm, 1.7 m 150 mm, 3.5 m
186004971
100 mm, 1.7 m 150 mm, 3.5 m
186004972
100 mm, 1.7 m

100 mm, 1.7 m
100 mm, 1.8 m
100 mm, 1.8 m
100 mm, 1.8 m
186004973
186004974
186004975
186004976
186004977
Package Name
NEW CSH C18 1.7 to 5 m Method Transfer kit
NEW CSH Phenyl-Hexyl 1.7 to 5 m Method Transfer kit
NEW CSH Fluoro-Phenyl 1.7 to 5 m
Method Transfer kit
BEH C18 1.7 to 5 m Method Transfer kit
BEH Shield RP18 1.7 to 5 m Method Transfer kit
BEH HILIC 1.7 to 5 m Method Transfer kit
HSS C18 1.8 to 5 m Method Transfer kit
HSS T3 1.8 to 5 m Method Transfer kit
HSS C18 SB 1.8 to 5 m Method Transfer kit
NEW CSH C18 1.7 to 3.5 m Method Transfer kit
NEW CSH Phenyl-Hexyl 1.7 to 3.5 m
Method Transfer kit
NEW CSH Fluoro-Phenyl 1.7 to 3.5 m
Method Transfer kit
BEH C18 1.7 to 3.5 m Method Transfer kit
BEH Shield RP18 1.7 to 3.5 m Method Transfer kit
BEH HILIC 1.7 to 3.5 m Method Transfer kit
BEH Amide 1.7 to 3.5 m Method Transfer kit
HSS C18 1.8 to 3.5 m Method Transfer kit
HSS T3 1.8 to 3.5 m Method Transfer kit
HSS C18 SB 1.8 to 3.5 m Method Transfer kit
NEW CSH C18 1.7 to 3.5 m
High Rs Method Transfer kit
NEW CSH Phenyl-Hexyl 1.7 to 3.5 m
NEW CSH Fluoro-Phenyl 1.7 to 3.5 m
BEH C18 1.7 to 3.5 m High Rs Method Transfer kit
BEH Shield RP18 1.7 to 3.5 m High
Rs Method Transfer kit
BEH HILIC 1.7 to 3.5 m High Rs Method Transfer kit
BEH Amide 1.7 to 3.5 m High Rs Method Transfer kit
HSS C18 1.8 to 3.5 m High Rs Method Transfer kit
HSS T3 1.8 to 3.5 m High Rs Method Transfer kit
HSS C18 SB 1.8 to 3.5 m High Rs Method Transfer kit
150 mm, 3.5 m

150 mm, 3.5 m
150 mm, 3.5 m
150 mm, 3.5 m
150 mm, 3.5 m
Chemistry
NEW CSH C18
NEW CSH C18
NEW CSH
Phenyl-Hexyl
NEW CSH
Phenyl-Hexyl
NEW CSH
Fluoro-Phenyl
NEW CSH
Fluoro-Phenyl
BEH C18
BEH C18
BEH C8
BEH C8
BEH Shield RP18
BEH Shield RP18
BEH Phenyl
BEH Phenyl
BEH HILIC
BEH HILIC
BEH Amide
BEH Amide
HSS T3
HSS T3
HSS C18
HSS C18
HSS C18 SB
HSS C18 SB
Particle
Size
Column
Length
Part No.
2.1 mm I.D.
Part No.
3.0 mm I.D.
1.7 m
1.7 m
50 mm
100 mm
186005571
186005572
186005573
186005574
1.7 m
50 mm
186005579
186005581
1.7 m
100 mm
186005580
186005582
1.7 m
50 mm
186005575
186005577
1.7 m
100 mm
186005576
186005578
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
1.8 m
50 mm
100 mm
50 mm
100 mm
50 mm
100 mm
50 mm
100 mm
50 mm
100 mm
50 mm
100 mm
50 mm
100 mm
50 mm
100 mm
50 mm
100 mm
186004044
186004045
186004046
186004047
186004048
186004049
186004050
186004052
186004053
186004054
186004807
186004808
186004055
186004056
186004057
186004058
186004137
186004138
186004691
186004692
186004693
186004694
186004695
186004696
186004697
186004698
186004699
186004700
186004809
186004810
186004701
186004702
186004703
186004704
186004705
186004709
**Each kit contains 3 columns from 3 different batches of material.
* Each kit contains one UPLC column and one HPLC column. The ACQUITY UPLC Columns
Calculator can be downloaded from the ACQUITY UPLC Online Community at www.waters.com/myuplc
VanGuard Pre-Column 3-Packs
ACQUITY UPLC Column In-Line Filter Unit

Description
In-line filter holder and six 0.2 m stainless steel replacement filters
Five 0.2 m stainless steel replacement filters
and End Nuts for 205000343
Chemistry
Particle
Size
Dimensions
Part No.
3 pack
NEW CSH C18

NEW CSH Fluoro-Phenyl
NEW CSH Pheny-Hexyl
1.7 m
1.7 m
1.7 m
2.1 x 5 mm
2.1 x 5 mm
2.1 x 5 mm
186005303
186005358
186005413
Description
BEH C18
BEH Shield RP18
BEH C8
BEH Phenyl
BEH HILIC
BEH Amide
HSS C18
HSS C18 SB
HSS T3
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.8 m
1.8 m
1.8 m
2.1 x 5 mm
2.1 x 5 mm
2.1 x 5 mm
2.1 x 5 mm
2.1 x 5 mm
2.1 x 5 mm
2.1 x 5 mm
2.1 x 5 mm
2.1 x 5 mm
186003975
186003977
186003978
186003979
186003980
186004799
186003981
186004136
186003976
Three 0.2 m Inlet/Outlet Frits for 3.0 mm ID UPLC Columns

One Inlet End Nut for 3.0 mm ID UPLC Column
One Outlet End Nut for 3.0 mm ID UPLC Column
102
Part No.
205000343
700002775
UPLC Column Hardware

Part No.
700004790
700003776
700003775
700004792
700004791
700003779
700003780
700003777
700003778
www.waters.com

T his selection of 12 x 32 mm vials are the most commonly ordered vials by customers using Waters ACQUITY UPLC Systems.
Clear
Amber
Max Recovery
Amber Max
300 L PP
750 L PP
Clear Glass
with Septumless Cap
600000668CV
600000669CV
600000670CV
600000755CV
186003886C
For PEEK and

Metal-Tipped Needles

186000307C
186000847C
186000327C
186000307DV
186000847DV
186000327DV
186004132C
186004133C
186004168C
186004132C
Combination Packs
186002639
186004112
186005221

Max
1600 L
1600 L
1100 L
1100 L
210 L
530 L
1600 L
Residual
165 L
165 L
22 L
22 L
20 L
70 L
165 L
Best Selection Vials

Limited Sample
Unlimited Sample
Light Sensitive

For the Amino Acid ACQUITY UPLC System and the MassTrack Amino Acid Analysis Solution System, use of Waters Total Recovery Vials are recommended, Part Number 186004631.
Waters LCMS Certified Vials (Suffix CV )

Waters LCMS certified vials are a continuation of our approac h to offer
products suitable for the demands of LCMS. We took an unbiased approach
in developing this product, looking for any ionized masses regardless of the
source. T he vials are tested by MS with specifications for total ion count
and presence of clusters in the high mass range. T he product introduced is
cleaner than any product we tested from vendors around the globe.
Waters LCGC Certified Vials (Suffix C)
Vials are usually manufactured by glass artisans and engineers who
dont understand t he requirements for t heir use in LC and GC. As
a manufacturer of autosamplers and c hemistry consumables, Waters
understands the dimensional and c hemical requirements of vials. We
reviewed the manufacturing process, anticipated possible problem areas,
and developed tests to ensure the delivery of problem-free products.
T he LC test, to ensure the delivery of residue-clean vials, is a radically
different form of test for the vials industry.
Deactivated Glass Vials (Suffix DV )
Deactivated glass vials eliminate adsorption of compounds onto the glass
surface when working with biological or pharmaceutical compounds, natural
products, pesticides and herbicides. T he surface modification is permanent,
resulting in an indefinite vial shelf life.
www.waters.com
ACQUITY UPLC Vial Descriptions

1



ACQUITY UPLC Vial Holder

Description
48-Well Vial Holder
Part No.
405003743
103

Plates for ACQUITY UPLC Systems
96-Well Plates
Plate
384-Well Plates
186002643
186002481
186002482
186002632
100
50
50
50
50
350 L
800 L
2 mL
250 L
100 L
186002483
186002483
186002484
186002788
186002788
186002788
186002788
186002788
186002789
186002789
186002789
186002789
186002789
35 L
15 L
20 L
15 L
15 L
Part No.
Max Volume
Residual Volume
15 L
Pack Size
Well Volume
186002631
Sealing Options
Residual Volume in ACQUITY at

default needle placement of 2 mm

Description
1860014381
186001437 (DV)2
650 L
186001436 (DV)2
850 L
15 L
186000349 (DV)2
650 L
15 L
186000855 (DV)2
850 L
15 L
186000857
186000856
Waters ACQUITY UPLC System Sample Manager

T he Waters ACQUITY UPLC System Sample Manager incorporates several
technology advancements. T he Sample Manager loop design and injection
modes maintains low dispersion and facilitates fast cycle times. T he system
performance is monitored and diagnosed through the console software.
It uses needle-in-needle sampling for improved ruggedness and a needle
calibration sensor for increased accuracy. A variety of sample holder
formats (vials or tubes) and microtiter plate formats (deep well, mid height)
can also be accommodated in a thermostatically-controlled environment.
Within the ACQUITY UPLC Sample Manager Instrument Method Editor, a

number of parameters can be customized for your specific task, including
depth, as shown here, to confer maximum sample format flexibility.
For further information on setting vial depth offsets, see the ACQUIT Y
UPLC Operators Guide (information documentation set for ACQUIT Y
UPLC Part Number 716001664) or visit the ACQUIT Y UPLC Sample
Manager Instrument Method Editor On-Line Help.
Waters ACQUITY UPLC System Sample Manager Version 1.30 and later
Sample Needle
Vial Depth
Typical 2 mL Vial
2 mm Default Offset
104
www.waters.com
ACQUITY UPLC CoLUmns And ConsUmAbLes

Heat Seals
Heat Seal for ACQUITY UPLC Systems
Clear Polyester Heat Seal
Heat Sealer
Part No.
115 Volt
186002786
240 Volt
186002787
Heat Sealer Operating Guidelines

For more information regarding heat sealers, download the heat sealer
manual, part number 720001330EN at http://www.waters.com/library
Temperature Range
Solvent Range
Clear Polyester
Heat Seal
From -80 C to 80 C
Good for Most

Lab Solvents
Aluminum Foil Laminate
From -200 C to 90 C
Good for Most

Lab Solvents
T he clear polyester heat seal is a non-conductive seal. For applications

requiring DMSO, the plates should be stored at 4 C.
Position the seal with the shiny side facing up. Apply heat using the Waters
heat sealer for 2 to 3 seconds in both directions following the instructions
found on page 9 in the heater manual, part number: 720001330EN.
Aluminum Foil Heat Seal
Recommendation
T he aluminum foil laminate heat seal is good for most solvents used
in laboratories. For applications requiring DMSO, the plates should be
stored at 4 C.
Best for Long Term

Storage
Position the seal with the white side facing up. Apply heat using the Waters
Heat Sealer for 2 to 3 seconds in both directions following the instructions
found on page 9 in the heater manual, part number: 720001330EN.
Both of these seals can be peeled off by hand. For plate storage, apply a
new unpierced seal or polypropylene cap mat.
Waters Online Vials Selector

T he Waters Vials Selector is designed to simplify the process of selecting the
best vial solutions for your system and application requirements.
T he selector offers vial options matc hing the criteria entered, suc h as the
system you are using, sample volume, detection method and light sensitivity
of analytes. As a registered user, you will be notified of all future updates.
For more information about Waters Certified Vials please visit
This interactive tool requires that the user has Adobe Flash Player 8.
Recommended Supplies for Use in Conjunction With the ACQUITY UPLC System
Filters
NEW Ostro Sample Preparation
Waters recommends you filter buffers and samples using a 0.2 m filter
prior to use. Filtration protects the column and instrument components
from a build-up of particulate matter, improving column lifetime and
minimizing system down time. 0.2 m GHP Filters are recommended for
filtering aqueous, non-polar solvents and proteins.
Ostro sample preparation products provide a novel solution for the cleanup
of phospholipids in plasma and serum. Requiring minimal to no method
development and using a simple protocol, this technology can be quickly
implemented in order to optimize your laboratorys workflow. Providing
cleaner, more reproducible extracts than competitive phospholipid removal
devices or techniques, the Ostro 96-well plate allows for more sensitive
analyses, increased sample throughput, and reduced instrument downtime.
Description
Part No.
Solvent filtration membranes 47 mm disc 100 pk*
186003524
13 mm Mini spike 100 pk (< 14 L hold up volume)
WAT097962
25 mm 50 pk (< 100 L hold up volume)
WAT097964
* Requires solvent filtration apparatus, WAT200543 or equivalent

Sirocco Protein Precipitation Plate enables high throughput in-well protein
precipitation. T he unique filter system with vented cap mat and patented
valve technology, allows the user to process samples in-well and collect
clean filtrate from smaller plasma volumes without transfer steps.
Description
www.waters.com
Part No.
NEW Ostro Sample Preparation 96-Well Plate (25 mg), single pack
186005518
Sirocco Protein Precipitation Plate, single pack
186003873
Sirocco Protein Precipitation Plate, 5 pack
186002448
105
[ Analytical Chromatography ]
Introduction ...........................................................................................................................................................................................108
Analytical Column Listing .......................................................................................................................................................................109
USP L Column Listing ...........................................................................................................................................................112
Physical Characteristics of HPLC
and UPLC Packing Materials ....................................................................................................................................................116
HPLC Columns Theory, Technology, and Practice ...................................................................................................................117
Product and Ordering Information
NEW XSelect Columns ..............................................................................................................................................................118
XBridge Columns ......................................................................................................................................................................122
SunFire Columns .......................................................................................................................................................................129
Atlantis Columns......................................................................................................................................................................132
NEW High Strength Silica (HSS) HPLC Columns .....................................................................................................................138
XTerra Columns ........................................................................................................................................................................140
Symmetry Columns ..................................................................................................................................................................144
Waters Spherisorb Columns .....................................................................................................................................................152
Nova-Pak Packings ....................................................................................................................................................................155
Bondapak/Bondapak Packings................................................................................................................................................158
Delta-Pak Columns ...................................................................................................................................................................159
Porasil/Porasil Silica Packings ...............................................................................................................................................160
Resolve Columns ......................................................................................................................................................................161
Shodex RSpak Polymer Reversed-Phase Columns...................................................................................................................162
Guard Products ..........................................................................................................................................................................163
Waters Cartridge and Guard Column Guide ............................................................................................................................164
Paired-Ion Chromatography (PIC Reagents) .............................................................................................................................166
Applications Specific
Sugar and Carbohydrate Analysis ...........................................................................................................................................167
Fatty Acid Analysis ..................................................................................................................................................................171
Pesticide Analysis ....................................................................................................................................................................171
Waters PAH Columns ...............................................................................................................................................................172
Triglycerides and Cholesterol Analysis ..................................................................................................................................173
Fermentation Analysis, Organic Acids,
Alcohols, and Carbohydrates ...................................................................................................................................................173
Ion Analysis ..............................................................................................................................................................................174
Transition Metal Analysis........................................................................................................................................................175
Lanthanide Analysis .................................................................................................................................................................175
Gas-Chromatography Packings.................................................................................................................................................................176
106
[ Preparative Chromatography ]
Column PerformanceIdentifying the Problem ....................................................................................................................................177
Waters OBD Column Design ....................................................................................................................................................................177
Column Stability and ReliabilityLong, Predictable Lifetimes ...........................................................................................................178
ScalabilityAnalytical Performance in a Prep OBD Column ..............................................................................................................178
Selecting the Column Packing .................................................................................................................................................................179
Waters AutoPurification System .............................................................................................................................................................179
Choosing Preparative Columns ................................................................................................................................................................180
NEW XSelect OBD Columns......................................................................................................................................................181
XBridge Prep OBD Columns .....................................................................................................................................................183
SunFire Prep OBD Columns ......................................................................................................................................................185
Atlantis Prep OBD Columns .....................................................................................................................................................187
XTerra Prep OBD Columns........................................................................................................................................................189
Symmetry Columns ..................................................................................................................................................................190
SymmetryShield Columns ........................................................................................................................................................190
Symmetry300 Columns ..........................................................................................................................................................190
SymmetryPrep Columns ...........................................................................................................................................................190
Waters Spherisorb Columns .....................................................................................................................................................191
Prep Nova-Pak HR Columns......................................................................................................................................................191
Bondapak/Porasil Columns ......................................................................................................................................................191
Delta-Pak Columns ...................................................................................................................................................................192
Preparative Bulk Material.........................................................................................................................................................192
Radial Compression Technology
Radial Compression Modules ...................................................................................................................................................193
Radial Compression Column Segments ..................................................................................................................................193
PrepPak Cartridges ....................................................................................................................................................................193
Accessories and Spare Parts ....................................................................................................................................................195
[ Supercritical Fluid Chromatography ]

Viridis SFC Columns .................................................................................................................................................................................196
Product and Ordering Information ..........................................................................................................................................................197
107
aNaLYTICaL ChROMaTOGRaPhY
Introduction
Featuring
CSH Technology
Choosing an HPLC column can be a very difficult process. T he Chromatographic Packing Material (particle brand name and functional group)
plays a major role in the success of a separation, both initially, when the
method is first developed, as well as long term, if the method will need
to be reproduced for many years.
Featuring
BEH Technology
T he Analytical Chromatography section of this catalog is structured in

two parts:
General/Technical Information
If you know the brand of column you need, go directly to the page shown in
the Product and Ordering Information Index for that brand.

Offering an uncomplicated introduction to the technology of liquid
chromatography [LC], with a focus on HPLC, this basic book uses clear
language, colorful diagrams, and a full glossary to acquaint readers
with basic concepts and terminology. T his primer is suitable for
younger science students as well as professionals new to LC.

T his tec hnology primer is designed to provide the reader with the
basic insight of how to be successful with hydrophilic interaction
c hromatography by understanding how the tec hnique works, the
parameters that impact retention and selectivity, as well as the
practical considerations necessary to successfully implement HILIC
within a chromatographic strategy.
108
Description
Part No.
715001531
715002531
www.waters.com
USP
Class No.
pH Range
(Room Temp.)
Temperature
Limits
Particle
Size
Pore
Size
Surface
Area
Carbon
Load
L1
1-11
Low pH = 80 C
High pH = 45 C
3.5, 5 m
130
185 m2/g
15%
NEW XSelect HPLC Columns
CSH C18
Selectivity Features: General purpose reversed-phase column that offers excellent pH stability and rapid mobile-phase
re-equilibration for method development. Charged Surface Hybrid (CSH) technology enables superior peak shape and increased
loading capacity for basic compounds.
Bonding: Trifunctional C18 ligand, fully end-capped, bonded to a CSH particle substrate.
O Si
O
L11
CSH
Phenyl-Hexyl
O
O Si
O
Low pH = 80 C
High pH = 45 C
3.5, 5 m
130
185 m2/g
14%
Selectivity Features: General purpose alternative selectivity ligand that provides pi-pi interactions with polyaromatic compounds,
while maintianing excellent reproducibility at pH extremes. Charged Surface Hybrid (CSH) technology enables superior peak shape
and increased loading capacity for basic compounds.
Bonding: Trifunctional C6 Phenyl ligand, fully end-capped, bonded to a CSH particle substrate.
O
O Si
O
L43
CSH
Fluoro-Phenyl
1-11
1-8
Low pH = 60 C
High pH = 45 C
3.5, 5 m
130
185 m2/g
10%
Selectivity Features: General purpose column that provides a very high degree of analyte selectivity, especially when using
low-pH mobile phases. Charged Surface Hybrid (CSH) technology enables superior peak shape and increased loading capacity
Bonding: Trifunctional propyl fluorophenyl ligand, non-endcapped, bonded to a CSH particle substrate.
XBridge HPLC Columns

L1
C18
1-12
L7
1-12
CH 3
O Si
O
CH
O Si 3
Polar Group
OOSi
18%
Low pH = 60 C
High pH = 60 C
2.5, 3.5,
5, 10 m
130
185 m2/g
13%
Polar Group
L1
2-11
O
O
O Si
OCH
O Si
Polar Group
O CH
3
Si
OO
O
OSi
O
O Si
O
O
O
O Si
CH 3
O
O Si
O
CH
OO Si 3
Polar Group
OOSi
O
Phenyl
185 m2/g
CH3
O Si
O
O
O Si
Shield RP18
130
O
O
O Si
O
O
Si
O CH
2.5, 3.5,
5, 10 m
O Si
O
O Si
O
O
O Si
O
C8
Low pH = 80 C
High pH = 60 C
O
Si
O CH
3
OOSi
CH3
O
O Si
O
Polar Group
Low pH = 50 C
High pH = 45 C
2.5, 3.5,
5, 10 m
130
185 m2/g
17%
Selectivity Features: Alternate selectivity as compared to straight chain C18, particularly with phenolic analytes.
Compatible with 100% aqueous-phase composition.
Bonding: Monofunctional embedded polar C18, fully endcapped, bonded to Ethylene Bridged Hybrid (BEH) substrate.
L11
1-12
Low pH = 80 C
High pH = 60 C
2.5, 3.5, 5 m
130
185 m2/g
15%
Selectivity Features: Excellent method development column for alternate selectivity, particularly in regard to polyaromatic
compounds. Unique level of pH stability for a Phenyl bonded phase.
Bonding: Trifunctional C6 Phenyl, fully endcapped, bonded to Ethylene Bridged Hybrid (BEH) substrate.
O
O Si
O
HILIC
O Si
O
L3
1-9
Low pH = 45 C
High pH = 45 C
2.5, 3.5,
5 m
130
185 m2/g
unbonded
Bonding: Unbonded Ethylene Bridged Hybrid (BEH) substrate.
O Si
O
www.waters.com
109
USP
Class No.
pH Range
(Room Temp.)
Temperature
Limits
Particle
Size
Pore
Size
Surface
Area
Carbon
Load
2-11
Low pH = 90 C
High pH = 90 C
3.5 m
130
185 m2/g
12%
XBridge HPLC Columns
Amide
O Si
O
Linker
NH 2
Selectivity Features: Rugged HILIC stationary phase designed to separate a wide range of very polar compounds. Especially good at
separating carbohydrates (saccharides) using high concentrations of organic modifier, elevated temperature and high pH. Compatible with all
modern detectors including MS, ELSD, UV and Fluorescence.
Bonding: Trifuncional amide bonded to Ethylene Bridged Hybrid (BEH) substrate.
L1
BEH130 C18
1-12
O
O Si
O
(Peptide Separation
Technology)
Low pH = 80 C
High pH = 60 C
3.5, 5, 10 m
130
185 m2/g
18%
Selectivity Features: pH and temperature stable, small pore, C18, UPLC column for peptides. Specifically QC tested with peptide map.
O
O Si
O
BEH300 C18
L1
OCH 3
Si
O Si
(Peptide Separation
Technology)
Polar Group
O
CH3
O
O
O Si
O SiCH
3
O
O
O Si
BEH300 C4
1-12
L26
C4 H 9
1-10
CH33
CH
OO SiSi
Polar
C4Group
H9
CH33
CH
(Oligonucleotide
Separation Technology)
300
90 m2/g
12%
Low pH = 80 C
High pH = 50 C
3.5 m
300
90 m2/g
8%
Selectivity Features: pH and temperature stable, wide pore, C4, UPLC column for proteins. Specifically QC tested with protein mixture.
Bonding: Proprietary monofunctional C4 bonding to Ethylene Bridged Hybrid (BEH) substrate.
O
O
O Si
O Si
O
O
L1
BEH C18
3.5, 5, 10 m
Selectivity Features: pH and temperature stable, wide pore, C18, UPLC column for peptides. Specifically QC tested with peptide map.
CH3
(Protein Separation
Technology)
Low pH = 80 C
High pH = 60 C
1-12
Low pH = 80 C
High pH = 60 C
2.5 m
130
185 m2/g
18%
Selectivity Features: pH and temperature stable, small pore, C18, UPLC column for synthetic DNA and RNA. Specifically QC tested
with synthetic DNA ladder.
O
O Si
O
O
O
O Si
O Si
O
O
SunFire HPLC Columns*

CH 3
O Si
Polar Group
CH3
L1
O
O Si
O
C18
2-8
Low pH = 50 C
High pH = 40 C
2.5, 3.5,
5, 10 m
100
340 m2/g
16%
Selectivity Features: General purpose method development column. Very high loading capacity, particularly for basic analytes
in low pH mobile phases. Ideally suited for purification and impurity profile assays.
Bonding: Difunctional C18, fully endcapped, bonded to high purity silica substrate.
Si
O
O
Si
O
OO
O Si Si
OO
L7
CH 3
C8
O Si
O CH
Si
Polar Group
Low pH = 40 C
High pH = 40 C
2.5, 3.5,
5, 10 m
100
340 m2/g
12%
Selectivity Features: General purpose method development column. Very high loading capacity, particularly for basic analytes
in low pH mobile phases. Less hydrophobic, therefore, less retentive than C18 for most analytes.
Bonding: Difunctional C8, fully endcapped, bonded to high purity silica substrate.
O
O Si
CH 3
O
O Si
2-8
Polar Group
CH3
O
O Si
O
O
O Si
O
O
O Si
O
110
www.waters.com
ANALYTICAL CHROMATOGRAPHY
USP
Class No.
pH Range
(Room Temp.)
Temperature
Limits
Particle
Size
L1
2-8
Low pH = 45 C
High pH = 45 C
3, 5, 10 m
Pore
Size
Surface
Area
Carbon
Load
100
330 m2/g
14%
Atlantis HPLC Columns*

O
O Si
O
O
O Si
O
T3
O
O Si
OCH
O Si
Polar Group
CH3
O
Selectivity Features: Retention of polar compounds, compatible with 100% aqueous mobile phases, superior stability under
low pH conditions. Specifically designed for enhanced retention of polar analytes.
Bonding: T3 (C18) bonding and endcapping, bonded to high purity silica substrate.
O Si
O
O
O Si
O
L3
1-5
CH 3
O Si
Polar Group
CH3
HILIC
Low pH = 45 C
High pH = 45 C
3, 5 m
100
330 m2/g
unbonded
Bonding: Unbonded high purity silica substrate.
O
O Si
O
O
OO Si
O OSi
O
Low pH = 45 C
3, 5, 10 m
100
330 m2/g
High pH = 45 C
Selectivity Features: Retention of polar compounds. Designed for compatibility with 100% aqueous mobile phases.
Bonding: Difunctional C18 bonding, fully endcapped, bonded to high purity silica substrate.
L1
C18
OO Si
OSi
O
O
O Si
O
CH 3
O Si
O
3-7
12%
Polar Group
Si 3
O CH
O
* Atlantis and SunFire HPLC columns do not offer a sub-2 m analog.

O
OO Si
OO Si
HSS HPLC Columns

O
CH 3
O Si
O
Polar Group
Si 3
O CH
O
HSS C18
O
OOSi
OO Si
L1
1-8
Low pH = 45 C
High pH = 45 C
3.5, 5 m
100
230 m2/g
15%
Selectivity Features: High performance C18 chemistry, increased retention, superior peak shape, resists acid hydrolysis at low pH.
Designed for UPLC separations where silica-based C18 selectivities are desired.
Bonding: High coverage trifunctional C18, fully endcapped, bonded to High Strength Silica (HSS) HPLC particle substrate.
OO Si
O
CH 3
O Si
CH3
HSS C18 SB
O
OOSi
OO Si
Polar Group
L1
www.waters.com
O
O Si
O
Low pH = 45 C
High pH = 45 C
3.5, 5 m
100
230 m2/g
8%
Selectivity Features: Unique, non-endcapped C18 chemistry designed specifically for method development scientists. Offers unique
Selectivity for Bases (SB) when operating under low pH conditions and transferability between UPLC and HPLC separations.
Bonding: Intermediate coverage tri-functionally bonded C18, no endcapping, bonded to High Strength Silica (HSS) HPLC particle substrate.
L1
HSS T3
2-8
2-8
Low pH = 45 C
High pH = 45 C
3.5, 5 m
100
230 m2/g
11%
Selectivity Features: Aqueous mobile-phase compatible HPLC column designed for extreme retention.
Combines polar compound retention with transferability between UPLC and HPLC separations.
Bonding: T3 (C18) bonding and endcapping, bonded to High Strength Silica (HSS) HPLC particle substrate.
111
USP L Column Listing

US Pharmacopeia LC L1 (C18) Column Designation Subclassification Testing by Brand Name*
Column Brand Name
Hydrophobicity
(k, Ethylbenzene)
Chelating
(Tailing Factor (TF), Quinizarin)
Silanol Activity
(k, Amitriptyline)
Silanol Activity
(TF, Amitriptyline)
Shape Selectivity
(Bonding Density, mol/m2)
1.63
1.30
2.54
1.09
2.23
1.7
1.87
1.0
2.0
2.54
1.09
2.23
1.5
2.5
2.25
1.65
0.8
2.0
1.63
1.30
1.0
1.5
2.0
1.10
1.36
1.36
1.07
1.19
1.1
0.98
6
2
1.36
1.07
1.19
7.5
1.2
1.7
1.5
no peak
no peak
1.10
1.36
1.2
1.1
no peak
3.79
2.37
5.96
22.2
6.50
5.1
5.32
7.5
6.3
5.96
22.2
6.50
4.6
6.3
5.1
3.1
23
11.5
3.79
2.37
1.7
3.3
35
1.10
1.13
1.41
7.2
3.15
2.4
1.35
4
1.9
1.41
7.2
3.15
3
1.1
1.7
1.2
3
7
1.10
1.13
1.1
1.3
8
3.1
3.3
3.2
1.6
1.6
1.6
1.6
1.1
3.6
3.2
1.6
1.6
2.7
3.5
3.2
3.3
1.7
2.6
3.1
3.3
2.3
2.2
2.8

ACQUITY UPLC BEH Shield RP18
ACQUITY UPLC HSS T3
Atlantis dC18
Atlantis T3
Bondapak C18
Delta-Pak C18 100
HSS C18
HSS C18 SB
HSS T3
Nova-Pak C18
SunFire C18
Symmetry C18
SymmetryShield RP18
Waters Spherisorb ODS-1
Waters Spherisorb ODS-2
XBridge C18
XBridge Shield RP18
XTerra RP18
XTerra MS C18
Resolve C18
* For use in C18 column brand name selection when referencing USP monographs which specify the characteristics/performance of the C18 column used in the method.
Choose a brand as close as possible to the referenced column values for the best chance of duplicating the analytical results of the method.
Waters Electronic Column Selectivity Chart

If you would like to learn more about UPLC
and HPLC column particle tec hnology (packing
materials), including better ways to choose packing
materials for your application needs, visit:
eSelectivity Chart Version 3.0

112
www.waters.com
USP L Column Listing

L1
Octadecyl silane (ODS or C18) chemically bonded to porous silica

or ceramic particles - 1.5 to 10 m in diameter. See new
subclassification table on previous page.
Brand
Particle Size
AccQTag Ultra
ACQUITY UPLC CSH C18
ACQUITY UPLC Shield RP18
nanoACQUITY UPLC BEH130
ACQUITY UPLC OST C18
ACQUITY UPLC HSS T3
Atlantis T3
Atlantis dC18
BioSuite PA-B
BioSuite PA-A
Bondapak C18
Bondapak C18 Radial-Pak
HSS C18
HSS C18 SB
HSS T3
Delta-Pak C18
Nova-Pak C18
Prep Nova-Pak HR C18
Resolve C18
SunFire C18
Symmetry C18
SymmetryPrep C18
Symmetry300 C18
SymmetryShield RP18
Waters Spherisorb ODS1
Waters Spherisorb ODS2
Waters Spherisorb ODSB
XBridge C18
XBridge Shield RP18
XBridge BEH130
XBridge BEH300
XBridge OST C18
XSelect CSH C18
XTerra MS C18
XTerra RP18
L2
1.7
1.7
1.7
1.7
1.7
1.7
1.7
1.8
1.8
1.8
3, 5, 10
3, 5, 10
3
3
10
10
3.5, 5,
3.5, 5,
3.5, 5
5
4, 6
6
5, 10
3.5, 5, 10
3.5, 5, 7
3.5, 5, 7
3.5, 5
3.5, 5
3, 5, 10
3, 5, 10
3, 5, 10
2.5, 3.5, 5, 10
2.5, 3.5, 5, 10
3.5, 5, 10
3.5, 5, 10
2.5
3.5, 5
2.5, 3.5, 5, 10
3.5, 5, 10
Type
Page
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
240
91
93
93
262
262
246
95
95
95
133
133
212
212
158
194
138
138
138
159
156
191
161
131
146
190
150
148
153
153
153
127
127
208
208
246
121
142
142
Octadecyl silane (ODS or C18) chemically bonded to silica gel

of a controlled surface porosity bonded to a solid spherical core 30 to 50 m in diameter.
Brand
Bondapak Prep C18
Particle Size
Type
50
Irregular
Page
191
L3
Porous silica particles - 1.5 to 10 m in diameter.
Brand
Particle Size
Type
Page
ACQUITY UPLC BEH HILIC

Atlantis HILIC Silica
BioSuite UHR SEC
BioSuite SEC
Nova-Pak
Porasil
Resolve
SunFire Silica
Waters Spherisorb
XBridge HILIC
1.7
3, 5
5, 8
7.5
6, 4
10
5, 10
5, 10
5, 10
2.5, 3.5, 5
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Spherical
Spherical
Spherical
Spherical
93
137
233
233
156
160
161
186
153
127
L4
Silica gel of a controlled surface porosity bonded to a

solid spherical core - 30 to 50 m in diameter.
Brand
Porasil Prep Silica
Particle Size
Type
Page
50
Irregular
192
L5
Alumina of controlled surface porosity bonded to a solid spherical

core - 30 to 50 m in diameter.
L6
Strong cation exchanger packing - sulfonated fluorocarbon polymer

coated on a solid spherical core - 30 to 50 m in diameter.
L7
Octyl silane (C8) chemically bonded to porous silica

particles - 1.5 to 10 m in diameter.
Brand
ACQUITY UPLC BEH C8
Nova-Pak C8
Resolve C8
Waters Spherisorb C8
SunFire C8
Symmetry C8
SymmetryShield RP8
SymmetryPrep C8
XBridge C8
XTerra MS C8
XTerra RP8
L8
Type
Page
1.7
4, 6
5, 10
3, 5, 10
3.5, 5, 10
3.5, 5, 7
3.5, 5
7
2.5, 3.5, 5, 10
2.5, 3.5, 5, 10
3.5, 5, 10
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
93
156
161
153
186
146
148
190
127
142
142
An essentially monomolecular layer of aminopropylsilane (NH2)

chemically bonded to totally porous silica gel support - 3 to 10 m
in diameter.
Brand
Bondapak NH2
High Performance Carbohydrate Analysis
Waters Spherisorb NH2
L9
Particle Size
Particle Size
Type
Page
10
3, 5
3, 5, 10
Irregular
158
170
153
Spherical
3 to 10 m irregular, totally porous silica gel having a chemically

bonded strongly acidic cation exchanger coating (SCX).
Brand
Spherisorb SCX
Particle Size
Type
Page
5, 10
Spherical
153
( ) - Denotes particle sizes available outside of L class.
www.waters.com
113
L10
Brand
Bondapak CN
Nova-Pak CN HP
Resolve CN
Waters Spherisorb CN
L11
L19
Nitrile groups (CN) chemically bonded to porous silica

particles - 3 to 10 m in diameter.
Particle Size
Type
Page
10
4
5, 10
3, 5, 10
Irregular
Spherical
Spherical
Spherical
158
156
161
153
Brand
Particle Size
Type
Page
ACQUITY UPLC CSH Phenyl-Hexyl

ACQUITY UPLC BEH Phenyl
Bondapak Phenyl
Nova-Pak Phenyl
XBridge Phenyl
Waters Spherisorb Phenyl
XSelect CSH Phenyl-Hexyl
XTerra Phenyl
1.7
1.7
10
4
2.5, 3.5, 5
3, 5, 10
3.5, 5
3.5, 5
Spherical
Spherical
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
99
99
158
156
127
153
121
141
L12
Particle Size
AccellPlus QMA
L13
Irregular
230
Particle Size
Type
3, 5, 10
Spherical
Page
154
Waters Spherisorb SAX
Particle Size
Type
5, 10
Spherical
Page
154
Hexylsilane (C6) chemically bonded to a totally porous

silica particle - 3 to 10 m in diameter.
Brand
Particle Size
Type
3, 5, 10
Spherical
Page
154
L16
Dimethylsilane (C2) chemically bonded to a totally porous

silica particles - 5 to 10 m in diameter.
L17
Strong cation exchange resin consisting of sulfonated, cross-linked

styrene divinylbenzene copolymer in the hydrogen form, 7 to 11 m
in diameter.
Brand
Fast Fruit Juice
IC-Pak Ion Exclusion
IC-Pak Cation
Shodex RSpak DC-613
L18
Particle Size
Type
Page
N/A
7
10
(6)
N/A
Spherical
Spherical
Spherical
173
174
175
162
Amino (NH2) and Cyano (CN) groups chemically bonded

to porous silica particles - 3 to 10 m in diameter.
Particle Size
Type
Page
9
(7)
Spherical
Spherical
170
170
Dihydroxypropane groups chemically bonded to porous

Brand
Particle Size
Type
Page
BioSuite 125, 250, 450
4, 5, 8, 10,
13, 17
Spherical
233
N/A
Irregular
Irregular
Irregular
Irregular
Irregular
Irregular
213
234
234
234
234
234
234
Particle Size
Type
Page
Spherical
Spherical
Spherical
Spherical
162
272
272
272
Insulin HMW P
Protein-Pak 60
Protein Pak 125
Protein-Pak 300SW
Protein-Pak KW -802.5
Protein-Pak KW -803
Protein-Pak KW -804
Shodex RSpak 613

Styragel HR 0.5, 1, 2, 3, and 4
Styragel HR 4E
Styragel 5E
L22
A cation-exchange resin made of porous polystyrene

with sulfonic acid groups - about 10 m in size.
Brand
Shodex RSpak DC 613
Shodex SP-0810
L23
10
10
10
7
7
7
A rigid, spherical styrene-divinylbenzene

copolymer - 5 to 10 m in diameter.
Brand
Page
Silica gel, 5 to 10 m in diameter having a chemically bonded,

strongly basic quaternary ammonium anion exchanger (SAX) coating.
Brand
L15
Type
Trimethylsilane (C1) chemically bonded to porous

Brand
L14
50
Sugar-Pak 1
Shodex SC-1011
L21
A strong anion exchanger packing made by chemically

bonding a quaternary amine to a solid silica spherical core 30 to 50 m in diameter.
Brand
Brand
L20
Phenyl groups chemically bonded to porous silica

particles - 1.5 to 10 m in diameter.
Strong cation exchange resin consisting of sulfonated,

cross-linked styrene divinylbenzene copolymer in the
calcium form - about 9 m in diameter.
Particle Size
Type
Page
7
6
8
Spherical
Spherical
Spherical
175
162
170
An anion exchange resin made of porous polymethacrylate

or polyacrylate gel with quaternary ammonium groups about 10 m in size.
Brand
Particle Size
Type
Page
BioSuite Q AXC
BioSuite DEAE
BioSuite Q-PEEK
IC-Pak Anion
Protein-Pak Q 8HR
10, 13
2.5, 10, 13
10
10
8
Spherical
Spherical
Spherical
Spherical
Spherical
226
226
226
174
227
L24
A semi-rigid hydrophilic gel consisting of vinyl polymers

with numerous hydroxyl groups on the matrix surface - 32
to 63 m in diameter.
L25
Packing having the capacity to separate compounds with a molecular

weight range from 100 to 5,000 (as determined by polyethylene oxide), applied to neutral, anionic and cationic water-soluble
polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated ether, (surface contained some residual carboxyl groups)
was found suitable.
Brand
Ultrahydrogel DP, + 120
Particle Size
Type
Page
10
Spherical
279
114
www.waters.com
L26
Brand
ACQUITY UPLC BEH300 C4

Delta-Pak C4
Particle Size
Type
Page
1.7
Spherical
Spherical
[100+300]
Spherical
Spherical
222
Symmetry300 C4
XBridge BEH300 C4
L27
L37
Butyl silane (C4) chemically bonded to porous silica particles 3 to 10 m in diameter.
3.5
3.5
160
150
222
Brand
Ultrahydrogel 250
L38
Ultrahydrogel
Particle Size
Type
Page
Porasil
37-55
Irregular
160
A multifunctional support which consists of a high purity, 100,

spherical silica substrate that has been bonded with anionic
(amine) functionality in addition to a conventional reversedphase C8 functionality.
L39
Particle Size
Type
Page
N/A
Spherical
170
Particle Size
Type
Page
N/A
Spherical
170
Particle Size
Type
Page
N/A
Spherical
170
A methacrylate-based size-exclusion packing

for water soluble samples.
Brand
Porous silica particles, 30 to 50 m in diameter.
Brand
L28
Packing having the capacity to separate proteins by molecular

size over a range of 2,000 to 40,000 daltons. It is a
polymethacrylate gel.
A hydrophilic-polyhydroxymethacrylate gel
of totally porous spherical resin.
Brand
Ultrahydrogel
L40
Cellulose tris-3,5-dimethylphenylcarbamate coated porous

silica particles, 5 to 20 m in diameter.
L29
Gamma alumina, reversed-phase, low carbon percentage by

weight alumina-based polybutadiene spherical particles 5 m in diameter with a pore diameter of 80.
L41
Immobilized a1-acid glycoprotein on spherical silica particles,

5 m in diameter.
L30
Ethyl silane chemically bonded to a totally porous silica

particle - 3 to 10 m in diameter.
L42
Octylsilane and octadecylsilane groups chemically

bonded to porous silica particles, 5 m in diameter.
L31
A strong anion-exchange resin-quaternary amine bonded on latex

particles attached to a core of 8.5 m macroporous particles having a
pore size of 2,000 and consisting of ethylvinylbenzene cross-linked
with 55% divinyl benzene.
L43
Pentafluorophenyl groups chemically bonded to silica particles,

5 to 10 m in diameter.
L32
A chiral-ligand exchange packing - L proline copper complex

covalently bonded to irregularly shaped silica particles - 5 to
10 m in diameter.
L33
Packing having the capacity to separate proteins of 4,000 to

400,000 daltons. It is spherical, silica-based and processed
to provide pH stability.
Brand
BEH200 SEC
L34
Particle Size
Type
Page
1.7
Spherical
217
Strong cation-exchange resin consisting of sulfonated cross-linked

styrene-divinylbenzene copolymer in the lead form, about 9 m
in diameter.
Brand
Shodex SP0810
Particle Size
Type
Page
N/A
Spherical
170
L35
Zirconium-stabilized spherical silica packing with a hydrophilic

(diol-type) molecular mono layer bonded phase having a pore
size of 150.
L36
3,5-dinitrobenzoyl derivative of L-phenylglycine covalently

bonded to a 5 m aminopropyl silica.
Brand
ACQUITY UPLC CSH Fluoro-Phenyl
XSelect CSH Fluoro-Phenyl
Particle Size
Type
Page
1.7
3.5, 5
Spherical
Spherical
99
99
L44
A multifunctional support, which consists of a high purity, 60,

spherical silica substrate that has been bonded with a cationic
exchanger, sulfonic acid functionality in addition to a convention
reversed-phase C8 functionality.
L45
Beta cyclodextrin bonded to porous silica particles,

5 to 10 m in diameter.
L46
Polystyrene/divinylbenzene substrate agglomerated with quaternary

amine functionalized latex beads, 10 m in diameter.
L55
A strong cation-exchange resin made of porous silica coated with

polybutadiene-maleic acid copolymer, about 5 m in diameter.
Brand
Particle Size
Type
IC-Pak C M/D
L59
Page
175
Packing having the capacity to separate proteins by molecular weight

over the range of 10 to 500 kDa. It is spherical (10 m), silica-based,
and processed to provide hydrophilic characteristics and pH stability.
Brand
Biosuite 125, 250, 450 Series
Particle Size
Type
Page
4-17
Spherical
233
Source: United States Pharmacopeia

www.waters.com
115
Physical Characteristics of HPLC and UPLC Packing Materials
Brand
AccQTag Ultra
ACQUITY UPLC CSH
Chemistry
C18
C18
Phenyl-Hexyl
Fluoro-Phenyl
ACQUITY UPLC BEH
C18
C8
Shield RP18
Phenyl
HILIC
Amide
ACQUITY UPLC BEH200
SEC
C18
C18
C4
ACQUITY UPLC HSS
T3 C18
C18
C18 SB
Atlantis
T3 C18
dC18
HILIC
Bondapak
C18
Phenyl
CN
NH2
Bondapak
C18
C18
Delta-Pak
C4
C18
C4
C18
HSS
T3 C18
C18
C18 SB
Nova-Pak
C18
C8
Phenyl
CN HP
Silica
Porasil
Silica
Porasil
Silica
Resolve
C18
C8
CN
Silica
SunFire
C18
C8
Silica
Symmetry
C18
C8
SymmetryShield
RP8
RP18
SymmetryPrep
C18
C8
Symmetry300
C18
C4
116
Particle Shape
Particle Size(s)
Pore Size*
Surface Area [m2/g]
Pore Volume [cc/g]
% Carbon Load
Endcapped
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Irregular
Irregular
Irregular
Irregular
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.8 m
1.8 m
1.8 m
3, 5, 10 m
3, 5, 10 m
3, 5 m
10 m
10 m
10 m
10 m
15-20 m
15-20 m
5, 15 m
5, 15 m
5, 15 m
5, 15 m
3.5, 5 m
3.5, 5 m
3.5, 5 m
4, 6 m
4 m
4 m
4 m
4, 6 m
10 m
15-20 m
5, 10 m
5 m
10 m
5, 10 m
2.5, 3.5, 5, 10 m
2.5, 3.5, 5, 10 m
5, 10 m
3.5, 5 m
3.5, 5 m
3.5, 5 m
5 m
7 m
7 m
3.5, 5 m
3.5, 5 m
135
130
130
130
130
130
130
130
130
130
200
130
300
300
100
100
100
100
100
100
125
125
125
125
125
300
100
100
300
300
100
100
100
60
60
60
60
60
125
125
90
90
90
90
100
100
100
100
100
100
100
100
100
300
300
185
185
185
185
185
185
185
185
185
185
216
185
90
90
230
230
230
330
330
330
330
330
330
330
330
100
300
300
125
125
230
230
230
120
120
120
120
120
330
330
200
200
200
200
340
340
340
335
335
335
335
335
335
110
110
0.70
0.70
0.70
0.70
0.70
0.70
0.70
0.70
0.70
0.70
high
0.70
0.66
0.66
0.70
0.70
0.70
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.70
0.70
0.70
0.30
0.30
0.30
0.30
0.30
1.00
1.00
0.50
0.50
0.50
0.50
0.90
0.90
0.90
0.90
0.90
0.90
0.90
0.90
0.90
0.80
0.80
17
15
14
10
17
13
17
15
unbonded
12
12
17
12
8
11
15
8
14
12
unbonded
9.8
9.3
6
4
10
3.5
7.3
17
2.6
6.8
11
15
8
7.3
4
4.6
3
n/a
n/a
n/a
10.2
5.1
3
n/a
16
12
unbonded
19.1
11.7
15
17
19.1
11.7
8.5
2.8
yes
yes
yes
no
yes
yes
yes
yes
n/a
n/a
n/a
yes
yes
no
yes
yes
no
yes
yes
n/a
yes
yes
yes
no
yes
yes
yes
yes
yes
yes
yes
yes
no
yes
yes
yes
yes
n/a
n/a
n/a
no
no
no
n/a
yes
yes
n/a
yes
yes
yes
yes
yes
yes
yes
yes
www.waters.com
Brand
Waters Spherisorb
XBridge
XBridge BEH130
XBridge BEH300
XTerra
XSelect CSH
Chemistry
Particle Shape
Particle Size(s)
Pore Size*
Surface Area [m2/g]
Pore Volume [cc/g]
% Carbon Load
Endcapped
Silica
ODS2
ODS
ODSB
C8
C6
C1
Nitrile
Amino
Phenyl
OD/CN
SAX, SCX
C18
C8
Shield RP18
Phenyl
HILIC
Amide
C18
C18
C4
RP18
RP8
MS C18
MS C8
Phenyl
C18
Phenyl-Hexyl
Fluoro-Phenyl
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
3, 5, 10 m
3, 5, 10 m
3, 5, 10 m
5 m
3, 5, 10 m
3, 5, 10 m
3, 5, 10 m
3, 5, 10 m
3, 5, 10 m
3, 5, 10 m
5 m
5, 10 m
2.5, 3.5, 5, 10 m
2.5, 3.5, 5, 10 m
2.5, 3.5, 5, 10 m
2.5, 3.5, 5 m
2.5, 3.5, 5 m
3.5 m
3.5, 5, 10 m
3.5, 5, 10 m
3.5 m
3.5, 5, 10 m
3.5, 5, 10 m
2.5, 3.5, 5, 10 m
2.5, 3.5, 5, 10 m
3.5, 5 m
3.5, 5 m
3.5, 5 m
3.5, 5 m
80
80
80
80
80
80
80
80
80
80
80
80
135
135
135
135
130
130
135
300
300
125
125
125
125
125
135
135
135
220
220
220
220
220
220
220
220
220
220
220
220
185
185
185
185
185
185
185
86
90
175
175
175
175
175
185
185
185
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.70
0.70
0.70
0.70
0.70
0.70
0.70
0.66
0.66
0.70
0.70
0.70
0.70
0.70
0.70
0.70
0.70
n/a
11.5
6.2
11.5
5.8
4.7
2.2
3.1
1.9
2.5
5
4
17.5
17.5
17.5
17.5
unbonded
12
17.5
12
8
15
13.5
15.5
12
12
15
14
10
n/a
yes
no
yes
yes
yes
no
no
no
no
yes
no
yes
yes
yes
yes
n/a
n/a
yes
yes
no
yes
yes
yes
yes
yes
yes
yes
no
* Nominal value
HPLC Columns Theory, Technology, and Practice

Uwe D. Neue, Waters Corporation, Milford, MA,
published by John Wiley & Sons
High-performance liquid chromatography and its derivative techniques have

become the dominant analytical separation tools in the pharmaceutical,
chemical, and food industries, environmental laboratories, and therapeutic
drug monitoring.
Although the column is the heart of the HPLC instrument and essential to its
success, until now no single book has focused on the theory and practice of
column technology.
HPLC Columns provides thorough, state-of-the-art coverage of HPLC

column technology for the practicing technician and academician alike.
Along with a comprehensive discussion of the chemical and physical
processes of the HPLC column, it includes fundamental principles,
separation mechanisms, available technologies, column selection criteria,
and special techniques.
Special features include:
Explanation of the underlying principles of HPLC columns
Methods for selecting columns
Practical advice on using and applying columns,

including examples
Section by M. Zoubair El Fallah on methods development
Special techniques, including preparative chromatography,

continuous chromatography, and the simulated moving bed
Troubleshooting
Description
Part No.
HPLC Columns Theory, Technology, and Practice
www.waters.com
WAT038216
117
NEW XSelect Columns

T he Next Generation of LC Column Performance
XSelect HPLC columns are the next evolutionary step in Waters LC column
technology. Based on Charged Surface Hybrid (CSH) Technology, this HPLC
column family offers the widest range of selectivity choices and superior
peak shape improvements for basic compounds when operated in acidic, low
ionic strength mobile phases (e.g., 0.1% formic acid).
ACQUIT Y UPLC CSH and XSelect columns offer method development
scientists in all application areas the widest range of selectivities of any
modern LC columns produced by Waters. T his was accomplished without
compromising suc h necessary performance attributes as superior peak
shape for basic compounds, low column bleed, excellent batc h-to-batc h
reproducibility, ultra-efficiencies and seamless transferability between
different particle sizes and column formats.
Advantages of CSH Tec hnology include:
Superior peak shape for basic compounds
Increased loading capacity
Rapid column equilibration after c hanging mobile-phase pH
Improved batc h-to-batc h reproducibility
Excellent stability at low pH
XSelect Column Chemistries

C18
CSH Chemistry
Ligand Type
Fluoro-Phenyl
F
O Si
O
O Si
O
Phenyl-Hexyl
O
F
O Si
O
Trifunctional C18
Trifunctional Propylfluorophenyl
Trifunctional C6 Phenyl
3.5, 5 m
3.5, 5 m
3.5, 5 m
2.3 mol/m2
2.3 mol/m2
2.3 mol/m2
Carbon Load*
15
10
14
End-cap Style
Proprietary
None
Proprietary
1-11
1- 8
1-11
Low pH=80 C
Low pH=60 C
Low pH=80 C
High pH=45 C
High pH=45 C
High pH=45 C
Available Particle Sizes

Ligand Density*
pH Range
Temperature Limits
* Expected or Approximate Values
118
XSelect Analytical Columns
www.waters.com
Engineered to Provide a Wider
Range of Selectivity
Reproducibility
As sample complexity increases, method developers require more diverse

chromatographic sorbents to separate coeluting sample constituents. Offering
a family of c hromatographic sorbents that provide diverse selectivities
was one of the design considerations for XSelect HPLC columns.
Waters continuing efforts to develop hybrid particle tec hnology lead to
the innovations behind CSH particle development. From controlled surface
charge modification to bonding of the ligand, each step of the development
was to maximize selectivity differences compared to current commercially available chromatographic packing materials. T he Selectivity factor
(S-value) measures how different (or similar) the selectivities of two column
c hemistries are under a given set of conditions. T he value is determined
by measuring and plotting the retention factors (k) of analytes run on two
columns under the same c hromatographic conditions. Higher selectivity
values indicate a higher degree of orthogonality.
One of the challenges in designing a stationary phase with large selectivity

differences is maintaining acceptable batch-to-batch reproducibility. Waters
R&D recognized that method developers need reproducible columns that will
maintain performance over the lifetime of the method independent. T his was
a key consideration for the XSelect column family. T he figure below is an
overlay of gradient separations on columns containing nine different batches
of XSelect CSH Fluoro-Phenyl materials. In these results, the batches include
three different particle sizes demonstrating that even across different
particle sizes, the same selectivity is maintained. T he unique XSelect FluoroPhenyl phase establishes a new expectation that highly selective stationary
phases can be reproducible.
XSelect CSH Fluoro-Phenyl 5 m
Selectivity (S) = 100 x
10
6
7
1-R2
15
CSH C18
XSelect CSH Fluoro-Phenyl 3.5 m
kg on XSelect CSH Phases
CSH Fluoro-Phenyl
CSH Phenyl-Hexyl
10
R2 = 0.76
S = 49
ACQUITY UPLC CSH Fluoro-Phenyl 1.7 m
0
0
10
15
kg on XBridge C18
15
BEH RP18
kg on XBridge Phases
BEH Phenyl
BEH C8
10
0.0
R2 = 0.97
S = 16
0
10
15
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0 min
ACQUITY UPLC CSH Fluoro-Phenyl (1.7 m) and XSelect CSH Fluoro-Phenyl (3.5 and 5 m)
reproducibility and scalability. Gradient separations on 2.1 x 50 mm columns containing nine different
batches of CSH Technology Fluoro-Phenyl representing three (1.7, 3.5, and 5 m) paricle sizes. Gradient:
A:15.4mMammonium formate, pH 3; B: acetonitrile; 5 to 90% B linear in 5 minutes. Temperature:
30 C. Injection volume: 5 L. Detection: 254 nm. Flow rate: 0.5 mL/min. Analytes: (1) thiourea;
(2) resorcinol; (3)metoprolol; (4) 3-nitrophenol; (5) 2-chlorobenzoic acid; (6) amitriptyline;
(7) diethylphthalate; (8)fenoprofen; (9) dipropylphthalate; (10) pyrenesulfonic acid. System: ACQUITY UPLC.
kg on XBridge C18
Plots of gradient retention factors (kg) on XSelect (top) or XBridge (bottom) columns versus
gradient retention factors on an XBridge C18 column. T he more scatter around the regression line,
the larger the selectivity differences.
www.waters.com
119
TFA Performance without TFA Suppression
Increased Analytical Loadability
Trifluoroacetic acid (TFA) is commonly used as a peak sharpening agent,

which is added as an acidic mobile-phase modifier to improve peak shape
for basic compounds. However, using T FA as an ion-pairing agent does
not come without its drawbacks. W hen an LC column is exposed to T FA,
it is difficult to completely remove it from the stationary phase. T his makes
method development difficult, since a column is often used with multiple
buffers/additives at different pH values. As a result, it can be difficult to
determine if the optimal chromatographic conditions that are due to previous TFA exposure. More importantly, for applications that use electrospray
ionization-mass spectrometry (ESI-MS), alternatives to TFA are being investigated since TFA can cause some analyte signal suppression in electrospray
positive (ES+) ionization at concentrations exceeding 0.01% and severe
analyte signal suppression in electrospray negative (ES-) ionization.
T he ability to inject large amounts of material onto analytical-scale

c hromatographic columns is important in application areas suc h as
stability indicating assays; metabolite ID and forced degradation studies;
API impurity profiling; and genotoxic impurities assessments. In these
analytical-column scale chromatographic assays, a large bolus of one or
more analyte(s) of interest is/are injected and the researcher determines the
presence or absence of small amounts of related compounds that can be
benign, toxic or deadly.
CSH Technology provides TFA peak shape performance without the use of
TFA (and its associated disadvantages). Improved peak shape performance is
realized with a more MS-friendly mobile-phase additive such as formic acid
(e.g., 0.1% HCOOH). Further, because formic acid does not ex hibit
the lingering c hromatographic effects of an ion-pairing agent, method
development is faster and more straightforward.
In these types of analyses, the peak(s) of interest are usually injected at a

concentration that overloads the column. Overload conditions are when the
peak shape degrades (i.e., peak width increases, efficiency/peak capacity
decreases, etc.) as a function of increasing analyte concentration. If one can
create a column with an increased column overload concentration threshold,
one can increase the sensitivity and utility of the assay. A narrower main
analyte peak imparts additional chromatographic space for the separation
and quantitation of closely-eluting, trace-level impurities or degradants. T he
increased loading capacity built into the CSH Technology-powered ACQUITY
UPLC CSH and XSelect columns provides sharper, narrower peaks for basic
compounds under the acidic, low ionic strength mobile-phase conditions
most commonly used in the afore mentioned application areas.
The increased and improved loadability that is possible with CSH Technology
permits the separation, identification and quantitation of closely eluting
impurities or degradants.
Columns Designed for Method Development

and Method Transferability
Literature
References
ACQUITY UPLC CSH and XSelect
HPLC Columns Brochure,
Charged Surface Hybrid (CSH) Technology and
Its Use in Liquid Chromatography W hite Paper,
Practical Applications of Charged Surface
Hybrid (CSH) Technology Application Note,
Preparative Optimum Bed Density
(OBD) Columns Brochure,
Interactive Waters Reversed-Phase
Column Selectivity Chart,
Many c hromatographic laboratories are now part of multi-national/

multi-site organizations that utilize LC systems from different vendors
with varying LC platform configurations and detection modes. From a
global business perspective, it is vital to be able to quickly and easily
develop robust methods that are not only compatible with all modern
c hromatographic detection modes but are also transferable to laboratories and sites that may operate different LC system platforms. ACQUIT Y
UPLC CSH and XSelect columns, as part of the ACQUIT Y UPLC H-Class
total system solution, were strategically created for the 21st-century
global c hromatographic marketplace.
Using CSH Tec hnology throughout the entire process, methods can be
developed quickly with ACQUITY UPLC CSH columns and UPLC Technology
and then transferred to preparative-scale OBD XSelect columns for isolation
and purification. T he purity of the isolated fraction(s) can then be measured/
confirmed using ACQUITY UPLC CSH columns and UPLC Technology.
Fast, robust analytical-scale separations can be easily and quickly developed
using UPLC Tec hnology, ACQUIT Y UPLC CSH columns and simple mobile
phase additives and then straightforwardly scaled and transferred to an
XSelect OBD preparative column.
120
www.waters.com
Dimension
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 10 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 75 mm
2.1 x 150 mm
3.0 x 20 mm
3.0 x 20 mm IS
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 20 mm
4.6 x 20 mm IS
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
2.1 x 10 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 20 mm
3.0 x 20 mm IS
3.0 x 30 mm
3.0 x 50 mm
3.0 x 100 mm
3.0 x 150 mm
3.0 x 250 mm
4.6 x 20 mm IS
4.6 x 20 mm
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
Type
Particle Size
C18
Fluoro-Phenyl
Phenyl-Hexyl
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186005249
186005250
186005251
1860052521
186005253
186005254
186005255
186005256
186005644
186005257
1860052582
186005259
186005260
186005261
186005647
186005262
186005263
1860052642
186005265
186005266
186005267
186005268
186005269
186005270
1860052711
186005272
186005273
186005274
186005275
186005276
1860052772
186005278
186005279
186005280
186005281
186005282
186005283
186005284
1860052852
186005286
186005287
186005288
186005289
186005290
186005291
186005304
186005305
186005306
1860053071
186005308
186005309
186005310
186005311
186005646
186005312
1860053132
186005314
186005315
186005316
186005649
186005317
186005318
1860053192
186005320
186005321
186005322
186005323
186005324
186005325
1860053261
186005327
186005328
186005329
186005330
186005331
1860053322
186005333
186005334
186005335
186005336
186005337
186005338
186005339
1860053402
186005341
186005342
186005343
186005344
186005345
186005346
186005359
186005360
186005361
1860053621
186005363
186005364
186005365
186005366
186005645
186005367
1860053682
186005369
186005370
186005371
186005648
186005372
186005373
1860053742
186005375
186005376
186005377
186005378
186005379
186005380
1860053811
186005382
186005383
186005384
186005385
186005386
1860053872
186005388
186005389
186005390
186005391
186005392
186005393
186005394
1860053952
186005396
186005397
186005398
186005399
186005400
186005401
Particle Size
C18
Fluoro-Phenyl
Phenyl-Hexyl
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
5 m
5 m
186005538
186005539
186005540
186005541
186005542
186005543
186005544
186005545
186005546
186005547
186005548
186005549
186005550
186005551
186005552
186005553
186005554
186005555
186005556
186005557
186005558
186005559
186005560
186005561
186005562
186005563
186005564
186005565
186005566
186005567
186005568
186005569
186005570
Requires 2.1 x 10 mm Universal Sentry

Guard Holder, Part No. WAT097958
Requires 3.0 x 20 mm/4.6 x 20 mm

Universal Sentry Guard Holder,
Part No. WAT046910
XSelect Method Validation Kits

Dimension
2.1 x 100 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 100 mm
4.6 x 150 mm
2.1 x 150 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
www.waters.com
121
XBridge Columns
A New Milestone in Chromatography
Maximizing Column Efficiency
First introduced in 1999 in XTerra columns, Waters patented organic/

inorganic Hybrid Particle Technology [HPT ] surmounted significant limitations of silica-based, reversed-phase packing materials, particularly their
hydrolytic instability at high pH. In 2005, second-generation HPT, branded
as BEH Technology, embodied in new ACQUITY UPLC BEH columns and in
Waters XBridge HPLC columns, marked a new milestone in chromatography.
One of the most important parameters in the design of the new hybrid
particle was to significantly improve the chromatographic equivalence to
state-of-the-art silica based materials. T he origins of band spreading, which
decreases separation efficiency, are described by the van Deemter equation:
With an order-of-magnitude improvement in high pH stability and a higher

level of chromatographic performance, BEH Technology columns define the
new benchmark for LC method development.
Data for both silica and BEH Technology packings, fitted to the van Deemter
equation demonstrate effective equivalence in efficiency. The c term for
XBridge C18 is virtually identical to that for the two state-of-the-art C18
silica columns, indicating that all these columns are comparable in mass
transfer characteristics.
h = a + b/u + cu
van Deemter Curve Comparison

h using N1/2 Ht
BEH Technology
Analyte:
Mobile Phase:
Temperature:
12
10
BEH Technology, the second generation patented hybrid particle*, is a

product of Waters commitment to continued investment in particle Research
and Development, providing reliable solutions to complex method development problems. Based upon crucial customer feedback, three goals for this
second-generation hybrid particle were considered paramount.
Maximize efficiency
Further improve high-pH stability
Improve column reliability
Decanophenone
70/30 ACN/water
30 C
Reduced van Deemter Plot Terms

a
b
c
XBridge C18 5m
Symmetry C18 5m
SunFire C18 5m
1.04
0.76
0.95
8.7
10.6
11.6
0.044
0.045
0.040
r2
0.998
0.999
0.997
20
40
60
80
u [uidp/Dm]
The reduced plate height, h, is a function of the reduced linear velocity, u, [both normalized for particle size]
and a, b, and c summarize the contributions of eddy diffusion, longitudinal diffusion, and the sum of
stationary- and mobile-phase mass transfer terms, respectively.
* Patent # 6,686,035 B2
122
XBridge Analytical Columns
www.waters.com
pH Stability
Improved Low pH Performance
Over 1000% Longer High pH Endurance
T he predominant cause of shortened column lifetime in low pH mobile

phases relates to the acid hydrolysis of the bonded phase. T his hydrolysis
can lead to significant changes in analyte retention time, making method
suitability requirements difficult to achieve.
XBridge columns have been specifically designed to be the most pH stable

high-performance chromatographic phases commercially available. Unlike
approaches which claim high pH resistance due to special surface modifications, XBridge columns have stability built in as part of the particle
synthetic process.
XBridge packings incorporate the use of well-characterized, state-of-theart, proprietary procedures for bonding and endcapping. Bonded phases
prepared using these procedures are much more stable and reproducible
at low pH than conventionally prepared materials and resist the typical
ligand hydrolysis failure. In an accelerated low pH stability test, XBridge
C18 columns show very little retention loss and exhibit a column lifetime
equivalent to that of a sterically hindered C18 silica-bonded phase.
Under accelerated pH 10 stability test conditions, a direct comparison

to some of the most popular chromatographic phases, claimed to have
extended high pH stability, clearly shows the XBridge C18 column lifetime
exceeding that of the best silica column by over 1000% with very little
degradation in chromatographic performance.
Accelerated Low pH Stability Tests of Competitive Columns
Accelerated High pH Stability Test of Competitive Columns
Probe: Methylparaben
XBridge C18
Zorbax SB-C18
XBridge Phenyl
80
Zorbax SB Phenyl
90
% Initial N5s
90
% Initial Retention
Analyte: Acenaphthene
110
100
XBridge C18
XTerra MS C18
Gemini C18
70
Luna C18(2)
Luna C18(2)
70
YMC Pro C18
50
Zorbax Extend C18
ACT Ace C18

60
Gemini C18
30
0
50
50
50
100
100
200 hours
Compounds
1. Uracil
2. Propranolol
3. Naphthalene
4. Acenaphthene
5. Amitriptyline
Hours in 1% TFA in H2O pH 1.0 at 80 C
USP Tailing Factors at pH 3 and pH 7
USP Tailing Factors
5
N=13600
Initial
N=13500
100 h
N=14100
200 h
N=11700
300 h
pH 3
Desipramine
XBridge Family
Amitriptyline
pH 7
Nortriptyline
Amitriptyline
150
Hours in 50 mM TEA, pH 10, 50 C
Capcell PAK MG II
C8
Phenyl
RP18
C18
Zorbax
SB-C18
12
16
20
24 Min.
Chromatograms, run at regular intervals during the high-pH lifetime study, verify
that 86% of the original XBridge column efficiency remains after 300 hours at pH 10
and elevated temperature, with little change in peak shape or retention time.
The combination of excellent particle and ligand stability as well as high chromatographic
efficiencies makes XBridge columns an ideal choice for low and intermediate pH methods.
www.waters.com
123
Column Lifetime and Buffer Choice
Efficient Method Development
T he c hoice of mobile phase buffer and concentration, particularly at

elevated pH, has a profound impact not only on c hromatographic peak
shape but also on column lifetime. In order to be successful, the method
development c hemist requires the flexibility to c hoose the appropriate
buffer for selectivity and detection technique.
The XBridge family of columns introduces a new level of chromatographic

performance and confidence to the method development chemist. Robust,
rugged methods can now be achieved across the entire pH range of 1-12,
simplifying the validation and method transfer process.
T he XBridge family of columns exhibit maximum stability in the widest

range of volatile and non-volatile buffer types.
Utilizing a simple method development protocol, consisting of XBridge

columns, two mobile phase pHs, and two organic solvents, even the most
challenging of sample mixtures can be quickly resolved.
Chromatographic Lifetime Comparisons

Phenomenex Gemini C18
Waters XBridge C18
XBridge Low pH in Methanol

XBridge Low pH in Methanol
C18
C18
30 Hours
300 Hours
N = 2218
N = 11700
2
5
2
3
3
12
16
20
24 Min.
10
20
30 Hours
13 Hours
300 Hours
27050
Hours
mM TEA, 50 C, pH 10
12
10
16
Waters 5XBridge C18
15
20
24 Min.
20 Min.
0
0
10
10
15
20 Min.
5
10
15
Phenomenex
Gemini
C18
10
15
30 mM KH2PO4, 30 C, pH 12.3
5
6
The development route to a final, robust LC method may require

investigation of many selectivity tools, such as bonded ligands, solvent
type, modifiers, temperature and most importantly pH. This route is
complicated further by the fact that modern column chemistries usually
are designed to excel in only a limited combination of these conditions,
making the selection of the most suitable column extremely difficult.
The XBridge family of columns is designed to eliminate this compromise
and deliver the flexibility to work under any mobile phase, temperature
and pH conditions, thus speeding up the process to an optimum and rugged
final method.
10
11
11
7,8
9
13
9,10
12
12
9,10
78
5
11
13
12
13
11
2
2
12
12
7
7
10
5
10
5
13
78
12
12
16
20 Min.
12
16
20 Min.
12
16
20 Min.
12
16
20 Min.
XBridge Low pH in Acetonitrile

XBridge Low pH in Acetonitrile
C18
C18
2
2
6
6
4
4
4,5
4,5
10
11
10
11
67
12
12
13
10
5
10
11,12
6
2
2
13
3
5
10
13
9
9
12
11,12
10
10
11
Phenyl
Phenyl
11
12
86 7
11
13
4
1
7
10
12
13
9
9
11
Shield RP18
2
Shield RP18
12
12
12
3
3
6
7
2
1
11
5 6
Phenyl
Phenyl
12
23
11
6
12
Shield RP18
Shield RP18
2
11
11
9,10
9,10
XBridge High pH in Acetonitrile

XBridge High pH in Acetonitrile
C18
C18
7
10
10
13
8
8
12
16
20 Min.
12
16
20 Min.
12
16
20 Min.
12
16
20 Min.
Literature
References
Waters XBridge HPLC
Columns Brochure,
A Review of Waters Hybrid Particle Technology
Part 2. Ethylene-Bridged (BEH TEchnology)
Hybrids and T heir Use in Liquid
Chromatography W hite Paper,
124
11
Phenyl
Phenyl
9
5
11
2
2
10
7,8
12
T he Universal Family of Columns
8,9
8,13
7
Shield RP18
Shield RP18
12
12
Method Development Flexibility
11
11
8,13
10
10
12
1
10
10
8,9
20 Min.
3
3
Phenyl
Phenyl
13 Hours
5
6
11
11
16.68
1,2
1,2
30 Min.
20 Min.
11 16.68
20
270 Hours
Shield RP18
Shield RP18
N = 2218
N = 11700
0
0
30 Min.
10
C18
C18
11
5 6
XBridge High pH in Methanol

XBridge High pH in Methanol
10
Utlizing XBridge HPLC Columns

for Method Development at pH
Extremes Application Note,
(OBD) Columns Brochure,,
www.waters.com
XBridge and Phosphate Buffer
With the introduction, and subsequent widespread adoption, of high purity
reversed-phase silica columns in the early 1990s, the method development chemist has been unable to freely utilize phosphate buffers (pH 7
and above) due to their extremely aggressive nature. The combination of
phosphate, intermediate pH and relatively low temperatures (40 C) is well
recognized to lead to short column lifetimes for most modern phases.
Phosphate buffers however do have quite desirable qualities for a non-MS
based assay:
Excellent UV transparency
Unique peak shape and selectivity characteristics
Good buffering capacity at multiple pKa values
XBridge C18, 4.6 x 100 mm, 3.5 m

A1: 30 mM potassium phosphate buffer (pH 2)
Acetonitrile
1.4 mL/min
Time
Profile
(min)
%A
%B
0.0
90
10
7.0
20
80
8.0
20
80
20 L
50 g/mL each in
80:20 Water:Methanol
30 C
UV @ 210 nm (pH 2,7);
220 nm (pH 12)
with 2996 PDA
Mobile Phase B:
Flow Rate:
Gradient:
Injection Volume:
Sample Concentration:
Temperature:
Detection:
Instrument:
The increasing productivity demands on the chromatography laboratory

present an additional tough challenge to the method developer. Once an
initial method has been established, optimization of the total cycle time
becomes the key parameter.
The availability of multiple particle sizes and dimensions allows the
optimization of the total cycle time without sacrificing resolution.
Methods can easily be transferred from HPLC to UPLC and from UPLC
to HPLC.
Maintaining Resolution with Constant Length/ Particle Size Ratio
Creating Selectivity Changes with Phosphate Buffer

Column:
Mobile Phase:
Particle Sizes and Dimensions

for Method Optimization
Mobile Phase A:
Mobile Phase B:
Isocratic:
Compounds
1. 1-methylxanthene
2. 1,3-dimethyluric acid
3. theobromine
4. 1,7-dimethylxanthene
0.1% FA in H2O
0.1% FA in ACN
95% A: 5% B
5 m-150 mm
Injection = 5.0 L
Rs(2,3) = 2.28
Flow Rate: 0.2 mL/min
4
Compounds
1. Doxylamine
2. Benzamide
3. Hydroxyisophthalic Acid
4. Doxepine
5. Flavone
6. Fenoprofen
10 Min.
3.5 m-100 mm
Injection = 3.3 L
Rs(2,3) = 2.32
HPLC
4 Min.
2.5 m-75 mm
Injection = 2.5 L
Rs(2,3) = 2.34
pH 2
0.4
0.8
1.2
1.6
2 Min.
1.7 m-50 mm
Injection = 1.7 L
Rs(2,3) = 2.29
pH 7
UPLC
0
2
6
pH 12
0.2
0.4
0.6
0.8
1.10 Min.
ACQUITY UPLC systems and BEH chemistries are specifically designed

to enable the quickest method development procedures, fastest cycle
times while delivering maximum resolution.
8 Min.
XBridge columns are unique in their ability to withstand these aggressive conditions
(even at pH 12) allowing complete flexibility for the method development chemist,
while maintaining the highest efficiency values associated with silica columns.
www.waters.com
125
XBridge Amide Columns
Since 2003, Waters has developed innovative stationary phases for
Hydrophilic Interaction Chromatography [HILIC] to overcome the challenge
of retaining and separating extremely polar compounds. Based on Waters
novel ethylene bridged hybrid [BEH] particle tec hnology, XBridge
Amide columns utilize a chemically stable, trifunctionally-bonded amide
phase, enabling a new dimension in stability and versatility for HILIC
separations. XBridge Amide columns offer the same selectivity as ACQUITY
UPLC BEH Amide columns, allowing scientists to transfer their separations
between HPLC and UPLC technology platforms.
In addition to enhanced retention of polar compounds, XBridge Amide
columns provide increased mass spectrometry response, direct compatibility
with sample preparation eluates [ PPT, LLE and SPE] as well as orthogonal
selectivity compared to reversed-phase materials, making HILIC an attractive alternative separation technique.
XBridge Amide Analysis of Ginsenoside Rb1

Column:
Part Number:
Mobile Phase :
Flow Rate:
Inj. Vol.:
Sample Diluent:
Column Temp.:
Needle Wash:
Detection:
Sampling Rate:
Time Constant:
Instrument:
Carbohydrate Analysis
One of the most abundant and diverse classes of compounds analyzed by
HILIC are carbohydrates [monosaccharides, disaccharides, oligosaccharides
and polysaccharides].
T he XBridge Amide column is an exceptional tool for the analysis of
carbohydrates, providing the following benefits:
T he 3.5 m particle size enables high resolution, high speed analysis

of carbohydrates in complex sample matrices while maintaining or
improving c hromatographic resolution.
Increased c hemical stability enables the use of high pH and high

temperature to collapse anomers without the loss of reducing sugars.
BEH particle tec hnology, in combination with a trifunctionally-bonded

amide phase, provides exceptional column lifetime, thus improving
assay robustness.
Unlike amine-based columns used for carbohydrate analysis, the

XBridge Amide column is not susceptible to Sc hiff-base formation,
thus improving quantitation accuracy.
XBridge Amide Analysis of Saccharides

Column:
Part Number:
Mobile P hase A:
Mobile P hase B:
Flow Rate:
Gradient:
XBridge Amide, 3.5 m, 4.6 x 150 mm

186004869
80/20 ACN/H2O
1.4 mL/min
11.5 L
MeOH
60 C
ACN/H2O 95/5
UV @ 203 nm
20 points/sec
0.1
Waters Alliance with 2998 PDA Detector
XBridge Amide, 3.5 m, 4.6 x 150 mm

186004869
0.4 mL/min
Profile
Time (min)
%A
%B
Initial
0
100
30.6
60
40
30.7
0
100
10 L
50/50 MeCN/H2O
35 C
ACN/H2O 75/25
Waters ACQUIT Y UPLC with ACQUIT Y UPLC TQD
ES2.8 kV
25 V (fructose, glucose); 15 V (sucrose, maltose) and 40 V (maltooligosacc harides [n=1 to 5])
120 C
350 C
500 L/Hr
179.2 (fructose, glucose); 341.3 (sucrose, maltose); 503.4, 665.5, 827.6, 989.7,
115 (maltooligosaccharides [n=1 to 5])
0.2 s
Inj. Vol.:
Sample Diluent:
Column Temp.:
Needle Wash:
Instrument:
Ionization Mode:
Capillary:
Cone Voltage:
Source Temp.:
Desolv. Temp.:
Desolv. Gas Flow:
SIR m/z:
Ginsenoside Rb1
Orento extract
Dwell Time:
Compounds:
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
5. Maltotriose
6. Maltotetraose
7. Maltopentaose
8. Maltohexa hose
9. Maltoheptaose
Ginsenoside Rb1
20 g/mL Ginsenoside Rb1 standard
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5 min
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
10 g/mL
4
6
2
7
126
10
12
14
16
18
20
22
24
26
28
30
32 min
www.waters.com
Si
Si
3
N
Lin
ke
r
Si
O
O
2.5, 3.5, 5 m
2.5, 3.5, 5 m
3.5 m
3.1 mol/m2
3.2 mol/m2
3.3 mol/m2
3.0 mol/m2
N/A
7.5 mol/m2
Carbon Load*
18%
13%
17%
15%
unbonded
12%
Endcap Style
Si
2.5, 3.5, 5, 10 m
O
Bonded Phase
Si
2.5, 3.5, 5, 10 m
2.5, 3.5, 5, 10 m
Ligand Density*
BEH Particle
Amide
Unbonded
BEH
Trifunctional
C6 Phenyl
Si
Monofunctional
Embedded Polar Group
H
C
Available Particle Sizes (m)
Si
Si
3
Si
H
C
O
Po
la
rG
ro
up
Trifunctional C8
Si
Trifunctional C18
Po
la
rG
ro
up
O
O
Si
O
O
Ligand Type
Si
Si
Po
la
rG
ro
up
Si
Si
O
Po
la
rG
ro
up
Si
XBridge Column Characteristics
Si
Proprietary
Proprietary
TMS
Proprietary
N/A
None
pH Range
1-12
1-12
2-11
1-12
1-9
2-11
Low pH Temp. Limits
80 C
60 C
50 C
80 C
45 C
90 C
High pH Temp. Limits
60 C
60 C
45 C
60 C
45 C
90 C
Pore Diameter*
130
130
130
130
130
130
Pore Volume*
0.7 mL/g
0.7 mL/g
0.7 mL/g
0.7 mL/g
0.7 mL/g
0.7 mL/g
Surface Area*
185 m2/g
185 m2/g
185 m2/g
185 m2/g
185 m2/g
185 m2/g
* Nominal Values

Dimension
1.0 x 50 mm
2.1 x 10 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
3.0 x 20 mm IS
3.0 x 20 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
4.6 x 20 mm IS
4.6 x 20 mm
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
2.1 x 10 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 20 mm IS
3.0 x 20 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 100 mm
3.0 x 150 mm
1
2
Type
Particle Size
C18
C8
Shield RP18
Phenyl
HILIC
Amide
Column
Guard
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
186003118
1860030561
186003201
186003084
186003085
186005626
186003087
1860030572
186003121
186003122
186005630
186003088
1860030582
186003089
186003090
186003124
186003126
186003127
186003128
1860030591
186003019
186003020
186003021
186003022
186003023
186003024
1860030602
186003025
186003026
186003027
186003028
186003164
1860030741
186003167
186003099
186003101
186005627
186003168
1860030752
186003169
186003170
186005631
186003172
1860030762
186003173
186003174
186003175
186003177
186003178
186003179
1860030771
186003180
186003046
186003047
186003048
186003049
186003181
1860030782
186003182
186003050
186003051
186003052
186003136
1860030651
186003139
186003091
186003092
186005628
186003140
1860030662
186003141
186003142
186005632
186003144
1860030672
186003145
186003096
186003146
186003148
186003149
186003150
1860030681
186003151
186003035
186003036
186003037
186003038
186003152
1860030692
186003153
186003039
186003040
186003041
186003306
1860033591
186003307
186003308
186003309
186005629
186003310
1860033602
186003311
186003312
186005633
186003313
1860033612
186003314
186003315
186003316
186003317
186003318
186003319
1860033621
186003320
186003321
186003322
186003323
186003324
186003325
1860033632
186003326
186003327
186003328
186003329
186004455
186004456
186004457
186004458
1860044592
186004460
186004461
186004429
1860044301
186004431
186004432
186004433
186004434
186004435
186004436
186004871
1860048571
186004858
186004859
186004860
186004861
186004862
186004863
186004864
Requires 2.1 x 10 mm Universal Sentry Guard Holder, Part No. WAT097958

Requires 3.0 x 20 mm/4.6 x 20 mm Universal Sentry Guard Holder, Part No. WAT046910
www.waters.com
127
Dimension
4.6 x 20 mm IS
4.6 x 20 mm
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
2.1 x 10 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 20 mm IS
3.0 x 20 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 100 mm
3.0 x 150 mm
3.0 x 250 mm
4.6 x 20 mm IS
4.6 x 20 mm
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
1
2
Type
Particle Size
C18
C8
Shield RP18
Phenyl
HILIC
Amide
Column
Guard
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186003029
1860030612
186003030
186003031
186003032
186003033
186003034
186003943
1860030621
186003107
186003129
186003108
186003109
186003110
186003130
1860030632
186003111
186003131
186003132
186003112
186003133
186003134
1860030642
186003135
186003113
186003114
186003115
186003116
186003117
186003183
1860030792
186003184
186003053
186003185
186003054
186003055
186003963
1860030801
186003186
186003187
186003011
186003012
186003013
186003188
1860030812
186003189
186003190
186003191
186003014
186003192
186003193
1860030822
186003194
186003015
186003195
186003016
186003017
186003018
186003154
1860030702
186003155
186003042
186003043
186003044
186003045
186003964
1860030711
186003156
186003157
186002999
186003002
186003003
186003158
1860030722
186003159
186003160
186003004
186003005
186003161
186003162
1860030732
186003163
186003006
186003007
186003008
186003009
186003010
186003330
1860033642
186003331
186003332
186003333
186003334
186003335
186003965
1860033661
186003336
186003337
186003338
186003339
186003340
186003341
1860033672
186003342
186003343
186003344
186003345
186003346
186003347
1860033682
186003348
186003349
186003350
186003351
186003352
186003353
1860044372
186004438
186004439
186004440
186004441
1860044421
186004443
186004444
186004445
186004446
186004447
186004448
1860044492
186004450
186004451
186004452
186004453
186004454
1860048652
186004866
186004867
186004868
186004869
186004870
Requires 2.1 x 10 mm Universal Sentry Guard Holder, Part No. WAT097958

Also Available in Preparative

Optimum Bed Density (OBD)
Columns, See Page 183
XBridge Column Method Validation Kits

Each Method Validation Kit contains 3 columns, each from a different batch.
Dimension
2.1 x 100 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 100 mm
4.6 x 150 mm
2.1 x 150 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
128
Type
Particle Size
C18
C8
Shield RP18
Phenyl
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
5 m
5 m
186003766
186003767
186003768
186003769
186003770
186003771
186003772
186003773
186003774
186003775
186003776
186003777
186003778
186003779
186003780
186003781
186003782
186003783
186003784
186003785
186003786
186003787
186003788
186003789
186003790
186003791
186003792
186003793
186003794
186003795
186003796
186003797
186003798
186003799
186003800
186003801
186003802
186003803
186003804
186003805
186003806
186003807
186003808
186003809
www.waters.com
SunFire C18 and C8 Analytical Columns

SunFire columns set the standard for the state-of-the-art bonded C18
and C 8 silica HPLC columns. Benefiting from years of researc h and
product development, SunFire columns represent the best in particle
and bonding expertise and deliver the industry-leading level of
Excellent Low pH Stability
Superior Peak Shape

With new bonding and new end-capping tec hnologies for the SunFire
columns, Waters has developed a sorbent with superior peak shape
performance. SunFire columns provide symmetrical peaks for improved
resolution and quantitation of acidic, neutral, and basic compounds at low
and intermediate pH ranges.
Isocratic Separation
Mobile Phase A:
Mobile Phase B:
Wavelength:
Flow Rate:
Injection Volume:
Temperature:
Instrumentation:
Column lifetime is improved by low pH stability superior

to that of many silica-based HPLC column brands
100
% of Initial Retention Time
90
SunFire C18
2
3
80
SunFire C18
TUSP 1.26
70
Compounds:
1. Uracil
2. Propranolol
3. Butylparaben
4. Naphthalene
5. Acenaphthene
6. Amitriptyline
35% 20 mM K2HPO4/20 mM KH2PO4, pH 7.0

65% Methanol
254 nm
1.0 mL/min
14 L
23 C
2487 Dual Wavelength Absorbance Detector
4.6 x 150 mm, 5 m

GL Sciences Inertsil ODS-3
ACT Ace C18
Phenomenex Luna C18 (2)

4.6 x 150 mm, 5 m
TUSP 1.35
60
Phenomenex Luna C18(2)
50
0
20
40
60
80
ACT Ace C18

4.6 x 150 mm, 5 m
TUSP 1.79
Hours in 1% TFA in H2O at 80 C

0
10
15
20
25
30
35
40 min
High Efficiency
A combination of proprietary, state-of-the-art silica synthesis, bonding,
end-capping, and packing technologies produce SunFire columns with high
efficiency. One important benefit of these efforts is greater sensitivity.
MS-Compatibility
SunFire columns are compatible with mass spectrometry applications,
providing sharp peaks, excellent sensitivity, high peak capacity, and
ultra-low bleed. In addition, the speed, excellent resolution, and low
backpressure offered by SunFire Intelligent Speed (IS) columns reduce
costs and analysis times.
Comparison of C18 5 m HPLC Columns

SunFire C18
Phenomenex Luna C18 (2)
T hermo Scientific HyPURITY Elite C18
ACT Ace C18
GL Sciences Inertsil ODS-3
YMC-Pro C18
Agilent Purosphere RP-18E
Agilent Zorbax Eclipse XDB C18
1.0
1.5
2.0
2.5
3.0
USP Tailing Factor (TUSP)
3.5
4.0
In this comparison test of columns packed with 5 m particles, SunFire C18 column has the lowest USP tailing
factor for the basic probe amitriptyline under the most demanding pH 7 mobile-phase conditions.
www.waters.com
SunFire Analytical Columns
129
Batch-to-Batch Reproducibility
product. SunFire columns have demonstrated superior reproducibility
over many years. Batc hes randomly selected over the past 4 years show
excellent reproducibility in the example below.
In establishing new analytical methods for the latest pharmaceutical

and biopharmaceutical products, the selection of a reproducible HPLC
column is essential. T he selected column needs to provide the same
c hromatographic results over the life of the method and the new drug
Batch 112 (2005)
Batch 118 (2007)
Batch 130 (2007)
Batch 134 (2008)
10
15
20
25
30
35
40 Min.
T his excellent reproducibility is a result of our commitment to maintaining the tightest specifications in the HPLC column industry. SunFire columns start with high purity raw materials,
and are produced using tightly controlled manufacturing processes and column packing procedures that provide todays scientists with the best, most reproducible HPLC columns available.
SunFire 2.5 m Columns

T his smaller particle size allows c hromatographers to gain improved
sensitivity and greater efficiency. SunFire columns with 2.5 m particle
size enable faster run times while maintaining the same resolution.
SunFire C18, 4.6 x 50 mm, 2.5 m

P = 1800 psi
Pc = 48
Rs2/3 = 3.5
Min
0.0
4.0
5.0
8.0
3
1
3
1
SunFire C18, 4.6 x 75 mm, 3.5 m

P = 1360 psi
Pc = 55
Rs2/3 = 3.8
SunFire C18, 4.6 x 100 mm, 5 m
P = 880 psi
Pc = 49
Rs2/3 = 3.6
130
%B
15
60
15
15
Columns:
%C
10
10
10
10
3
4
%A
75
30
75
75
0.5
1.0
1.5
2.0
2.5
3.0
3.5
0.5
1.0
1.5
2.0
2.5
3.0
3.5
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
Min 4.0%A
0.0
75
6.0 4.030
7.5
75
12.0 75
5.0 Min.
4.5
%B
4.5
15
4.5
60
15
15
%C
5.0
10
10
5.0
10
10
Min.
Min.
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5 Min.
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5 Min.
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
Min %A
0.0
75
8.0
30
10.0 8.075
16.0 75
%B
15
60
15
9.0
15
Injection Volumes:
Column Temperature:
Detection:
Sampling Rate:
Instrument:
modifications)
%C 7.5 Min.
10
10
10
10.0
10
Min.
1.0
2.0
3.0
4.0
5.0
6.0
7.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0 Min.
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0 Min.
Mobile Phase A:
Mobile Phase B:
Mobile Phase C:
Flow Rate:
Sample:
SunFire C18, 4.6 x 50 mm, 2.5 m

SunFire C18, 4.6 x 75 mm, 3.5 m
SunFire C18, 4.6 x 100 mm, 5 m
Water
Acetonitrile
100 mM CH3COONH4, pH 5.0
1 mL/min
Sulfanilamide, sulfathiazole, sulfamerazine,
sulfamethoxazole and sulfaquinoxaline
dissolved in water at concentration of 10 g/mL each
5, 7, 10 L
Ambient
UV @ 270 nm
5 pt/sec
Waters Alliance 2695 with 2996 PDA (no instrument
www.waters.com
Physical Characteristics
Chemistry
Particle Size
Particle Shape
Pore Size
Carbon Load
End-capped
C18
2.5, 3.5, 5, 10 m
Spherical
100
16%
Yes
C8
2.5, 3.5, 5, 10 m
Spherical
100
12%
Yes
Silica
5, 10 m
Spherical
100
unbonded
N/A
SunFire 2.5 m Analytical Columns

Dimension
1.0 x 50 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
3.0 x 20 mm IS
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
4.6 x 20 mm IS
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
Particle Size
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
2.5 m
SunFire 5 m Analytical Columns

C18
C8
186003392
186003397
186003399
186003401
186005634
186003403
186003407
186003409
186005636
186003411
186003415
186003417
186003419
186003394
186003398
186003400
186003402
186005635
186003404
186003408
186003410
186005637
186003412
186003416
186003418
186003420
Particle Size
C18
C8
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
186002526
186002528
186002531
186002532
186002533
186002534
186002535
186002686
186003254
186002542
186002543
186002544
186002549
186002550
186002551
186002552
186002553
186002554
186002705
186002706
186002697
186002709
186002710
186002711
186002712
186002701
Custom
186002719
186002720
186002721
186002699
186002728
186002729
186002730
186002731
186002732
Dimension
1.0 x 150 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 20 mm IS
3.0 x 50 mm
3.0 x 100 mm
3.0 x 150 mm
3.0 x 250 mm
4.6 x 20 mm IS
4.6 x 30 mm
4.6 x 50 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
SunFire 3.5 m Analytical Columns

Dimension
1.0 x 50 mm
1.0 x 150 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 20 mm IS
3.0 x 30 mm
3.0 x 50 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 20 mm IS
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm

Dimension

River Water by LC/MS/MS Applications Note,
www.waters.com
Particle Size
2.1 x 100 mm MV Kits

186002529
186002537
186002538
186002539
186002540
186002541
186002685
186002545
186002546
186002547
186002548
186002555
186002556
186002557
186002558
186002559
186002560
C8
186002707
186002698
186002714
186002715
186002716
186002717
186002702
186002723
186002724
186002725
186002726
186002700
186002734
186002735
186002736
186002737
186002738
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
C18
186002674
186002675
186002676
186002677
186002678
186002679
186002680
C8
186002739
186002740
186002741
186002742
186002743
186002744
186002745
SunFire Analytical Guard Columns

Sentry Guard Columns (2/pk)
Dimension
2.1 x 10 mm
3.0 x 20 mm
4.6 x 20 mm
2.1 x 10 mm
3.0 x 20 mm
4.6 x 20 mm
2.1 x 10 mm
4.6 x 20 mm
3.0 x 20 mm
A New State-of-the-Art Silica C18 Column

for Improved Peak Shape of Basic
Analytes Applications Note,
Literature Reference WA40526EN
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
C18
SunFire Method Validation Kits
Literature
References
SunFire Columns-T he Standard in Silica-Based
HPLC Column Performance Brochure,
Particle Size
3
4
Particle Size
C18
C8
2.5 m
2.5 m
2.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
1860033953
1860033963
1860034064
1860034144
1860027083
1860027184
1860027274
1860027133
1860027334
1860027224
1860034054
1860034134
1860025303
1860026814
1860026824
1860025363
1860026844
1860026834
Requires 2.1 x 10 mm Universal Sentry Guard Column Holder, Part No. WAT097958
Requires 3.0 x 20 mm Universal Sentry Guard Column Holder, Part No. WAT046910
Also available in Preparative Optimum Bed Density

(OBD) Columns, See Page 185
131
Atlantis Columns
T he Atlantis family of HPLC columns was created to solve one of the
most challenging chromatographic problems, retaining polar compounds.
T hrough historical experience and synthetic innovation, Atlantis columns
lead t he industry in providing exceptional performance, versatility,
retention and chromatographic stability for polar compounds, while also
affording balanced retention for broad analyte mixtures.
For retention and separation of polar compounds via reversed-phase HPLC,
Atlantis T3 columns set the standard for polar compound retention. Atlantis
T3 columns are a universal, silica-based, reversed-phase C18 line of HPLC
columns that not only retain and separate small, water-soluble polar
organic compounds, but also provide superior performance across a wider
pH range. Atlantis columns are the culmination of over 30 years of bonded
phase researc h and are a vast improvement upon the highly successful
Atlantis dC18 line of columns. For reversed-phase applications, Atlantis T3

columns should be considered the first choice when developing a separation
of polar (and non-polar) compounds. T he advantages of T3 bonding
tec hnology is extended to UPLC in the ACQUITY UPLC HSS T3 columns.
Atlantis HILIC silica columns retain and separate very polar compounds,
suc h as actives and metabolites using Hydrophilic-Interaction Chromatography (HILIC). W hy HILIC? HILIC provides retention when traditional
chromatographic techniques will not work. HILIC also offers advantages
such as improved LC/MS response, direct compatibility with solid-phase
extraction (SPE) solvents and complementary selectivity as compared to
reversed-phase HPLC.
Guide to Retaining Polar Compounds
Base
Atlantis T3
or
XBridge C18
Yes
Is the polar
compound an
acid, neutral
or base?
Can it be
retained by
reversed-phase?
Can it be
retained by
reversed-phase?
No
Yes
Sufficient mass
spectrometry
sensitivity?
Acid or Neutral
Yes
Atlantis T3
No

or
XBridge HILIC
Insufficient
retention?
Yes
No
Key:
Atlantis T3 = low pH (pH 3), high aqueous (>95%)
XBridge C18 = high pH (pH10) high aqueous (>95%)
Alternate separation
technique, such as
ion-pair or
ion-exchange
Atlantis HILIC Silica = low pH (pH 3), 10 mM ammonium formate with 0.2% formic acid in 95% ACN:5% H2O
XBridge HILIC = mid pH (pH 5), 10 mM ammonium acetate with 0.02% acetic acid in 95% ACN:5% H2O
132
Atlantis Analytical Columns
www.waters.com
T3 Bonding
T he superior performance of Atlantis T3 HPLC columns and the ACQUITY
UPLC HSS T3 columns is a result of Waters advanced T3 bonding process.
T3 bonding utilizes a trifunctional C18 alkyl phase bonded at a ligand
density that promotes polar compound retention and aqueous mobile-phase
compatibility. T he proprietary T3 endcapping process is muc h more
effective than traditional trimethyl-silane (TMS) endcapping.
Retention and Peak Shape Under 100% Aqueous Conditions

10 mM Ammonium Formate, pH 3.0, 30 C
Compounds
1. T hiourea
2. 5-Fluorocytosine
3. Adenine
T his unique combination of bonding and endcapping provides superior

polar compound retention and aqueous compatibility while also enhancing
column performance, lifetime, peak shape, and stability.
4. Guanosine - 5- monophosphate
5. T hymine
5
Atlantis T3
3,5
Agilent Zorbax SB-Aq
5
Thermo Aquasil C18
3
1
2,4
Phenomenex Synergi Polar-RP

1
3,4
5
Phenomenex Synergi Hydro-RP
Shiseido Capcell Pak C18 AQ

0
9 Min.
Atlantis T3 columns provide 100% aqueous compatibility, superior polar compound

retention and excellent peak shape for acids, neutrals and bases.
Long Column Lifetimes at Low pH

T he creation of highly retentive, reversed-phase C18 columns for polar
compound retention involves bonding at a ligand density that is less
hydrophobic and, therefore, more compatible with the weak, highly aqueous
mobile phases required for retaining polar compounds. W hen exposed to
strongly acidic mobile phases (i.e. < pH 2.0), traditional C18 columns can
exhibit bonded phase hydrolysis resulting in gradual loss of retention, loss
of efficiency as well as change in selectivity.
Atlantis T3 columns resist ligand cleavage by utilizing a trifunctional
attac hment of t he C 18 phase to t he particle surface, t hus providing
exceptional column life at low pH.
Superior Low pH Stability

20 hr Exposure to 0.5% TFA at 60 C
Atlantis T3
SunFire C18
Shiseido Capcell Pak C18 AQ
Agilent Zorbax Eclipse Plus
Atlantis dC18
Phenomenex Aqua
GL Sciences Inertsil ODS-SP
10
20
30
40
50
60
70%
% Loss of Retention for Methyl Paraben
T3 bonding allows Atlantis T3 columns to provide long

column lifetimes under harsh, low pH conditions.
www.waters.com
133
Improved pH 7 Performance
At pH 7, poor peak shape for amine-containing bases and shortened
column lifetimes are encountered when using intermediate ligand density
C18 columns designed for polar compound retention. Poor peak shape is due
to secondary interactions with unreacted silanols that remain present after
bonding and endcapping. T he proprietary T3 endcapping procedure reacts
with more of these active silanols thereby dramatically improving peak
shape for bases. Shortened column lifetimes are due to the dissolution of
the silica particle substrate by the high pH mobile phase. T he more effective
and efficient T3 bonding helps protect the silica substrate from dissolution,
thus providing longer column lifetimes.
Improved Peak Shape for Bases at pH 7
Atlantis T3
GL Sciences Inertsil ODS-SP
Shiseido Capcell PAK C18 AQ
Phenomenex Aqua
Atlantis dC18
Agilent Zorbax SB-Aq
T hermo Aquasil C18
Did not elute

1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
Amitriptyline USP Tailing Factor
When compared to other reversed-phase polar compound retention columns,

Atlantis T3 columns provide superior pH 7 peak shape.
Balanced Retention of Diverse Analyte Mixtures

W hen faced with the task of analyzing a number of compounds encompassing a broad range of polarity, few options exist that will result in adequate
retention of polar species without excessive retention of the hydrophobic components. In drug discovery, a large number of candidates need
to be screened, often with little or no previous c haracterization of their
c hemical properties. Many polar candidates are often overlooked due to
their elution in the void space of the column, while hydrophobic species
may be permanently bound to the stationary phase.
Exceptional Performance for Hydrophobic Compounds

Column:
Mobile Phase A:
Mobile Phase B:
Mobile Phase C:
Mobile Phase D:
Flow Rate:
Gradient:
Atlantis T3 columns are designed to deliver exceptional performance for

an expansive range of compound polarities, thus being ideally suited as a
universal reversed-phase column for open access environments.
Atlantis T3, 4.6 x 150 mm, 5 m

H2O
ACN
1% HCOOH, pH 2.3
MeOH
2 mL/min
Time
Profile
(min)
%A
%B
%C
0.00
55
17.5
10
4.00
35
27.5
10
6.00
9
40
10
15.00 9
40
10
16.00 55
17.5
10
20.00 55
17.5
10
10 L
30 C
UV @ 280 nm
Injection Volume:
Column Temp.:
Detection:
%D
17.5
27.5
41
41
17.5
17.5
Compounds:
1. Propyl gallate
2. 2,4,5-Trihydroxybutyrophenone
3. Tert-butylhydroquinone
4. Butylated hydroxyanisole
5. 2,6-Di-tert-butyl-4-hydroxymethylphenol
6. Octyl gallate
7. Butylated hydroxytoluene
8. Lauryl gallate
4
1
2
5
134
10
12
14 Min.
www.waters.com
ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
ACQUIT Y UPLC HSS (High Strength Silica) columns contain the first
and only 100% silica particle designed, tested and intended for use
in applications up to 15000 psi/1000 bar. T he ACQUIT Y UPLC HSS
particle is not an HPLC particle. High pore volume HPLC particles do not
posses the mechanical stability necessary to withstand the high column
packing and operating pressures of UPLC separations.
Superior Retention of Polar Compounds

Column:
T he first ligand chemistry in this new UPLC-certified column family utilizes

T3 bonding in order to retain and separate polar organic compounds.
ACQUIT Y UPLC HSS T3 columns possess the superior polar-compound
retention, aqueous mobile-phase compatibility and ultra-low MS bleed
of Atlantis T3 columns.
Compounds
1. Norepinephrine
2. Epinephrine
3. Dopamine
4. 3,4- Dihydroxyphenylacetic acid (DOPAC)
5. Serotonin (5-HT)
6. 5-Hydroxy-3-indoleacetic acid (5-HIAA)
7
7. 4-Hydroxy-3-methoxyphenylacetic acid (HVA)
ACQUITY UPLC HSS T3

2.1 x 100 mm
10 mM CH3COONH4, pH 5.0 6
ACN
5
0.438 mL/min
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Isocratic1Mobile
3
Phase Composition:
2
Injection Volume:
Temperature:
Detection:
0
Instrument:
2% B
0.7 L
30 C
UV @ 280 nm
1
ACQUITY UPLC System with
ACQUITY UPLC 2996 PDA
4 Min.
5
1
4 Min.
1
3
Easier Method Transfer

Since Atlantis T3 HPLC and ACQUIT Y UPLC HSS T3 columns utilize T3
bonding, LC methods can be transferred from HPLC to UPLC separations.
Because Atlantis and HSS particles are different substrates, some method
modification and/or optimization may be required. A compelling benefit of
UPLC technology is the ease and speed at which this method optimization
can occur since UPLC separations offer greater resolution in less time.
T3 Bonding Enables HPLC to UPLC Transfer

HPLC Separation on
Atlantis T3
7
Moving from X Bridge HPLC to ACQUIT Y U PLC BEH columns is nearly

seamless, as the hybrid particle substrates differ only in particle size.
However, because of T3 bonding it should be straightforward to make
simple adjustments to successfully transfer a separation from an
Atlantis T3 HPLC column to an ACQUIT Y UPLC HSS T3 column.
6
4
2
3
1
UPLC Separation on
1 UPLC
ACQUITY
1
2
3
4HSS5 T3 6
0.2
4
5 n Tim e
80% Ru
ction!!
du
Re
0.6
0.8
1.0
0.4
9 Min.
1.4 Min
1.2
1
6
2
3
0
0.2
0.4
1. Cytosine
2.77
2. 5-Fluorocytosine
3.58
4.56
3. Uracil
3.93
1.71
4. 5-Fluorouracil
4.59
3.41
5. Guanine
5.92
7.76
6. T hymine
7.63
11.01
7. Adenine
8.39
4.70
Similar Selectivities
USP
Resolution
Retention
Time (min)
9 Min.
Compound
Compound
Retention
Time (min)
USP
Resolution
1. Cytosine
0.34
2. 5-Fluorocytosine
0.44
5.02
3. Uracil
0.48
1.50
4. 5-Fluorouracil
0.56
3.17
5. Guanine
0.80
8.16
6. T hymine
1.25
14.65
7. Adenine
1.33
3.50
5
0.6
0.8
1.0
1.2
1.4 Min
T3 bonding allows for the transfer of HPLC methods to UPLC.
www.waters.com
135
Hydrophilic-Interaction
Chromatography (HILIC)
Retention Mechanisms in HydrophilicInteraction Chromatography (HILIC)
HILIC is an alternative c hromatographic tec hnique that offers complementary selectivity to RP and often retains very polar species that cannot
be retained by traditional means. Atlantis HILIC Silica columns were
designed to retain very polar, organic molecules that are too polar
to retain by R P. 1 Unlike the highly aqueous mobile phases required
for polar retention in R P separations, Atlantis HILIC Silica columns
employ highly volatile (>80% organic) mobile phases whic h are ideal
for mass spectrometry (MS) response and sensitivity. Additionally,
direct compatibility with high organic SPE eluates dramatically increases
the number of samples that can be handled, thus substantially increasing
sample throughput.
H2O
H
N
ACN/H2O
(90:10)
H
N
O
Si
NH3
NH3
Si
Si
Si O-
O
H
O
Si
Si
O
O
Si O H
O H
H
O-H
O-
Si
Si
HILIC retention mec hanisms are a complex combination of partitioning,

ion-exc hange and hydrogen bonding, resulting in enhanced retention
for polar analytes.1,2 Atlantis HILIC silica columns, used in combination
with high organic (>80% acetonitrile) mobile phases, result in retention of analytes that are simply too polar to retain by traditional R P
c hromatography.
1
E.S. Grumbach et al LCGC N. Am. 2004, 22, 1010-1023
E.S. Grumbach et al J. Sep. Sci. 2008, 31, 1511-1518
Enhanced Mass Spectrometry Response
Si
H
O
Si
Si
Si
O
O
Si
Si
O
O
Si
O-
O
Si
H-O
OH-O
Si
Si
Si
Si
O
Si
Si
O
Si O
Si
O
Si
Si
O
O
O
Si
Si
O-
O-
Si
Si
Si
Si
The combination of partitioning and weak cation-exchange results in

retention of polar bases with Atlantis HILIC Silica columns.
Reach Lower Limits of Detection
Utilization of HILIC has grown in popularity, primarily due to the extensive

adoption of mass spectrometry as a detector and the necessity of improving sensitivity for the quantitation of polar analytes. Unlike reversedphase whic h utilizes high aqueous mobile phases to induce retention,
HILIC employs an acetonitrile-rich mobile phase. T his high organic mobile
phase is easily desolvated, resulting in improved ionization efficiency and
mass spectrometry response.
For these reasons, HILIC has become a popular technique for bioanalytical
laboratories.
Si
O
O-H
Si
O-H
Si
O
Si
H2O
O-
Acetonitrile/Water (90:10)
H
O
H
H
H
Si
HO H
Si
Water
NH +
O
H-O
O-H
Si
O-
O
N
O-H
Si
Si O
O-
NH +
Si
O
H
N
Si
90% ACN
H
N
Si
OH
H3C
HO
NH
C3H
HO
CH3
NH
1. Bamethan
CH3
2. Albuterol
OH
OH
100
1.84
1
Reversed-Phase
2
2 18
100
0 50% ACN
1.0
2.0
3.0
4.0
5.0 min
1
1.84
2.18
0
1.53
100
2
HILIC
%
95 50% ACN
1.92
0
0.5
136
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0 min
www.waters.com
Dimension
1.0 x 50 mm
1.0 x 150 mm
2.1 x 10 mm
2.1 x 15 mm
2.1 x 20 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 75 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 20 mm IS
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
3.9 x 20 mm
3.9 x 50 mm
3.9 x 100 mm
3.9 x 150 mm
4.6 x 20 mm
4.6 x 20 mm IS
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
1.0 x 50 mm
1.0 x 150 mm
2.1 x 10 mm
2.1 x 15 mm
2.1 x 20 mm
2.1 x 20 mm IS
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 20 mm IS
3.0 x 50 mm
3.0 x 100 mm
3.0 x 150 mm
3.0 x 250 mm
3.9 x 20 mm
3.9 x 50 mm
3.9 x 100 mm
3.9 x 150 mm
4.6 x 20 mm
4.6 x 20 mm IS
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
Type
Particle Size
T3
dC18
HILIC Silica
Column
Column
Guard
Direct Connect
Guard
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Guard
Cartridge
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Column
Column
Guard
Direct Connect
Guard
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Guard
Cartridge
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Column
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186003713
186003714
1860037561
186003715
186003716
186003717
186005652
186003718
186003719
186003720
186003721
186005653
186003722
186003723
1860037573
1860037583
186003724
186003725
186003726
186003727
186003728
186003729
186003730
186003731
1860037591
186003732
186003733
186003734
186003735
186003736
186003737
186003738
186003739
186003740
186003741
1860037603
1860037613
186003742
186003743
186003744
186003745
186003746
186003747
186003748
186001279
186001283
1860013771
186002064
1860013812
186002058
186001287
186001291
186001295
186001299
186002060
186001389
186001303
186001307
1860013133
1860013854
186001393
186001317
1860013213
186002062
186001325
186001329
186001333
186001337
186001342
186001281
186001285
1860013791
186002065
1860013832
186002059
186001289
186001293
186001297
186001301
186002061
186001391
186001305
186001309
186001311
1860013153
1860013874
186001395
186001319
1860013233
186002063
186001327
186001331
186001335
186001340
186001344
186001346
186002003
1860020051
186002007
186002009
186002011
186002013
186002015
186002017
186002019
1860020213
1860020233
186002025
186002027
186002029
186002031
186002004
1860020061
186002008
186002010
186002012
186002014
186002016
186002018
186002020
1860020223
1860020243
186002026
186002028
186002030
186002032
186002033
Literature
References
Atlantis Columns Brochure,
Atlantis Columns Applications Notebook,
Atlantis T3 and ACQUITY UPLC
HSS T3 Columns Toss Sheet,
Topics in Liquid Chromatography: Part 1.
Designing a Reversed-Phase Column for Polar
Compound Retention W hite Paper,
Column Selectivity Chart
Also available in Preparative

Optimum Bed Density
(OBD) Columns, See Page 187
1 Requires
Sentry Guard Holder, Part No. WAT097958

Sentry Guard Holder, Part No. 186000262
3 Requires Sentry Guard Holder, Part No. WAT046910
4 Requires Cartridge Fittings, Part No. WAT037525
2 Requires
Atlantis Columns Method Validation Kits

Dimension
4.6 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
www.waters.com
Particle Size
T3
dC18
HILIC Silica
3 m
5 m
5 m
186003751
186003754
186003755
186002312
186002311
186002313
186002315
186002314
186002316
137
NEW High Strength Silica (HSS) HPLC Columns
Seamless Scalability Across T hree Particle Sizes
Mobile Phase A:
Mobile Phase B:
Injection volumes:
Temperature:
Detection:
Instruments:
Same chemistries (HSS C18, C18 SB and T3)

as ACQUITY UPLC HSS columns
HSS C18, 1.8 m, 2.1 x 50 mm (Part Number 186003532)

0.20
Glimepiride
Flow Rate
%A
%B
95
5
5
95
5
95
95
5
95
5
0.15
Seamless transferability between HPLC

and UPLC Technology
0.10
0.05
0
As large corporations implement UPLC Technology throughout their worldwide organizations there is a transition period during which older HPLC
systems are being phased out and replaced with ACQUITY UPLC systems.
T his transition does not take place overnight, however. During this transition period, c hromatographic methods that are developed using UPLC
Tec hnology must also be compatible with older HPLC systems. To make
this UPLC Tec hnology adoption strategy successful, one must be able to
seamlessly scale between UPLC Technology, HPLC and Preparative LC. T his
is only possible if chromatographic column platforms with HPLC and UPLC
particle sizes exhibit the same selectivities. BEH Technology is the particle
substrate platform tec hnology behind the industry-leading ACQUIT Y
UPLC BEH and XBridge HPLC column families and is a primary reason
behind the success of UPLC Technology. In 2006, Waters commercialized
ACQUIT Y U PLC High Strengt h Silica (HSS) columns based upon t he
1.8 m HSS UPLC particle substrate. Customers who have used and developed methods using ACQUITY UPLC HSS columns require HPLC particle
sizes and columns in order to help with UPLC Technology implementation.
1.7 m, 1.8, m
TRANSFERABILITY
0.5
1.0
1.5
2.0
2.5
3.0
3.5

0.20
Glimepiride
3.5 m and 5 m HSS particle sizes
(mL/min)
0.6
0.6
0.6
0.6
0.6
0.15
0.10
0.05
0
0
10
11
12
24
26
13

0.20
Glimepiride
Time
(min)
0.00
5.0
6.0
6.01
7.0
0.1% TFA
0.1% TFA in acetonitrile
30 L (4.6 x 150 mm, 5 m)
20 L (4.6 x 100 mm, 3.5 m)
2.1 L (2.1 x 50 mm, 1.8 m)
40 C
UV@230 nm
Alliance 2695 and 2996 PDA (HPLC)
ACQUITY UPLC and PDA (UPLC)
0.15
0.10
0.05
0
0
10
12
14
16
18
20
22
28
30 Min.
Waters developed the HSS HPLC column family in response to customer demands for ACQUITY UPLC HSS
selectivities in HPLC particle and column formats. The result: for a given HSS chemistry, HSS HPLC columns
provide the same selectivity across all particles sizes..
T he overwhelming success of UPLC Tec hnology in the marketplace has

forced column manufacturers to quickly develop sub-2 m particle columns
of their own (with varying degrees of success). T he fastest, easiest route
to market was for particle manufacturers to modify their HPLC particle
synthesis and sizing process to produce sub 2-m particles. Subsequent
customer testing has revealed that these processes along with column
manufacturing modifications were often unsuccessful. T he main complaint
voiced by customers who evaluated these new sub-2 m particle columns is
lack of transferability between HPLC and sub-2 m particle sizes.
W hy did Waters develop sub-2 m particle size columns first?
3.5 m, 5 m
2.5, 3.5, 5, 10 m
HSS HPLC columns join the BEH Technology-based XBridge HPLC columns as a
second seamless chromatographic migration path to and from UPLC Technology.
138
High Strength Silica (HSS) HPLC Columns
It is an easier and more straightforward process to produce larger (HPLC)

particles and chemistries by scaling up a sub-2 m particle manufacturing
process than it is to try and adapt an HPLC particle manufacturing process
to produce efficient, pressure-tolerant sub-2 m particles. Since Waters is
a primary manufacturer w ho produces its own particles, c hemistries
and column hardware, the same scientists and engineers who develop and
produce UPLC column products also produce the XSelect, XBridge and
HSS HPLC column analogs.
www.waters.com
Scale Between Preparative, Analytical and UPLC Separations with HSS Particles
HSS C18 SB, 5.0 m, 10 x 50 mm,

1
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
(Part Number 186004760)
Paroxetine
0.005
0.000
4.0
4.5
2
5.0
5.5
6.0
6.5
6.9 min
Analytical HPLC Separations
HSS C18 SB, 5.0 m, 4.6 x 150 mm,
0.010
AU
UPLC System:
Preparative HPLC Separation

0.010
0.005
Paroxetine
Detection:
HPLC System:
0.1% TFA
30 C
50:50 MeOH:H2O
Related Compound
1: 0.002 mg/mL
Paroxetine; 0.2 mg/mL
Related Compound
2: 0.002 mg/mL
UV@295 nm
Alliance 2695
with 2996 PDA
ACQUITY UPLC
with PDA
AU
Mobile Phase A:
Mobile Phase B:
Temperature:
Sample Diluent:
Concentrations:
0.000
0
10
12
14
16
18
20
20.8 min
HSS C18 SB, 3.5 m, 4.6 x 100 mm
Commercializing the sub-2 m particle columns

for BEH and HSS particle substrates first ensures
that regardless of particle size, a method can be
seamlessly transferred between HPLC
and UPLC Technology.
0.005
0.000
0
Paroxetine
AU
0.010
9.7 min
UPLC Separation
HSS C18 SB, 1.8 m, 2.1 x 50 mm

1
0.005
0.000
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
Paroxetine
AU
0.010
1.8
2.0
2.2
2.4
2.5 min
High-Strength-Silica (HSS) HPLC Analytical Columns

Dimension
3.0 x 20 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 75 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 20 mm
4.6 x 30 mm
4.6 x 50 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
4.6 x 20 mm
4.6 x 50 mm
4.6 x 150 mm
4.6 x 250 mm
1
Type
2-pk1
Guard Cartridge
Column
Column
Column
Column
Column
Guard Cartridge 2-pk1
Column
Column
Column
Column
Column
Guard Cartridge 2-pk1
Column
Column
Column
Particle Size
HSS C18
HSS C18 SB
HSS T3
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
186004764
186004765
186004766
186005642
186004762
186004763
186004769
186004771
186004772
186004767
186004768
186004770
186004774
186004852
186004773
186004775
186004745
186004746
186004747
186005643
186004743
186004744
186004750
186004752
186004753
186004748
186004749
186004751
186004755
186004757
186004754
186004756
186004782
186004783
186004784
186005641
186004780
186004781
186004787
186004789
186004790
186004785
186004786
186004788
186004792
186004794
186004791
186004793
Literature
References
High Strength Silica (HSS)
HPLC Columns Toss Sheet,
ACQUITY UPLC Column Strategies Brochure,
High-Strength-Silica (HSS) HPLC Preparative Columns

Dimension
10 x 10 mm
10 x 50 mm
10 x 100 mm
10 x 150 mm
2
Type
Guard Cartridge
Prep Column
Prep Column
Prep Column
2-pk2
Particle Size
HSS C18
HSS C18 SB
HSS T3
5 m
5 m
5 m
5 m
186004776
186004778
186004779
186004777
186004758
186004760
186004761
186004759
186004795
186004797
186004798
186004796
Requires 10 x 10 mm Cartridge Holder, Part No. 289000779
www.waters.com
High Strength Silica (HSS) HPLC Columns
139
XTerra Columns
T he Most Successful Column in Over 30 Years
of Stationary-Phase Technology
T he universal acceptance by pharmaceutical scientists in drug discovery,
method development, and isolation and purification has enabled XTerra
columns to become the fastest selling and most successful column product
in the world of chromatography. T he reason: the enabling science of Hybrid
Particle Tec hnology. Drug discovery scientists benefit from the DMSO
injection-resistance of the rugged XTerra particle. Method development
scientists are able to operate at whatever pH is necessary for optimal
selectivity due to XTerra columns wide pH range. Isolation and purification
scientists can load up to 60x more material per injection because of the
high mass loading capacity of XTerra columns.
In Hybrid Particle Technology, one out of every three silanols is

replaced with a methyl group during synthesis. This hydrophobicity is
distributed throughout the entire structure of the particle backbone.
The result is a rugged hybrid (inorganic/organic) particle that can be
operated at high speeds, high temperatures, and high pH. The presence
of 33% fewer residual silanols (after endcapping and bonding) also
means that XTerra columns give exceptionally sharp, high-efficiency peaks
Effect of XTerra Column Chemistry on Selectivity and Retention
Column:
Mobile Phase A:
Mobile Phase B:
Mobile Phase C:
Gradient:
4.6 x 150 mm, 5 m

H2O
MeOH
50 mM HCOOH, pH 2.45
Time
Profile
(min)
%A
%B
0.0
40
50
20.0
25
65
35.0
25
65
%C
10
10
10
Flow Rate:
Injection Volume:
Temperature:
Detection:
Instrument:
1.0 mL/min
15 mL
30 C
UV @ 254nm
Waters Alliance 2695,
996 PDA
Peaks:
1. Suprofen
2. Tolmetin
3. Naproxen
4. Fenoprofen
5. Ibuprofen
6. Diclofenac
Packing
Particle
Shape
Particle
Size(s)
Pore
Size
Carbon
Load
Endcapped
XTerra MS C18
XTerra MS C8
Spherical
Spherical
2.5, 3.5, 5, 10 m
2.5, 3.5, 5, 10 m
125
125
15.5%
12.0%
yes
yes
XTerra RP18
XTerra RP8
Spherical
Spherical
3.5, 5, 10 m
3.5, 5, 10 m
125
125
15.0%
13.5%
yes
yes
XTerra Phenyl
Spherical
3.5, 5 m
125
12.0%
yes
XTerra MS C8
O
Si
XTerra RP8
CH3
O
Si
CH3
Hybrid Particle Technology

For over 30 years, scientists have been forced to work within certain
boundaries when performing HPLC separations. Restrictions on speed,
resolution, pH, temperature, and loading capacity were imposed upon
the chromatographer by limitations of the stationary phase material.
The patented * Hybrid Particle Technology of XTerra columns allows
chromatographers to break these boundaries and realize the full potential of
their analytical and preparative separations.
XTerra Phenyl
Si
CH3
O
O
CH2 CH
CH3
10
15
20
25 Min.
Patent # 6,686,035 B2
The column stationary phase chemistries of XTerra columns are key
components in an overall method development plan.
140
XTerra Analytical Columns
www.waters.com
Ordering Information
XTerra Capillary and Narrowbore Columns
Dimension
Type
75 m x 50 mm
75 m x 100 mm
75 m x 150 mm
100 m x 50 mm
100 m x 100 mm
100 m x 150 mm
150 m x 50 mm
150 m x 100 mm
150 m x 150 mm
300 m x 50 mm
300 m x 100 mm
300 m x 150 mm
300 m x 50 mm
300 m x 100 mm
300 m x 150 mm
1.0 mm x 50 mm
1.0 mm x 50 mm
1.0 mm x 100 mm
1.0 mm x 150 mm
2.1 mm x 10 mm
2.1 mm x 10 mm
2.1 mm x 15 mm
2.1 mm x 15 mm
2.1 mm x 15 mm
2.1 mm x 15 mm
2.1 mm x 20 mm
2.1 mm x 20 mm
2.1 mm x 20 mm
2.1 mm x 20 mm
2.1 mm x 20 mm
2.1 mm x 30 mm
2.1 mm x 30 mm
2.1 mm x 50 mm
2.1 mm x 50 mm
2.1 mm x 50 mm
2.1 mm x 50 mm
2.1 mm x 50 mm
2.1 mm x 100 mm
2.1 mm x 100 mm
2.1 mm x 100 mm
2.1 mm x 100 mm
2.1 mm x 150 mm
2.1 mm x 150 mm
2.1 mm x 150 mm
2.1 mm x 150 mm
2.1 mm x 250 mm
2.1 mm x 250 mm
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Column
Column
Column
Column
Guard
Guard
Direct Connect Column
IS Column
IS Column
Guard
IS Column
Guard
Column
Column
Column
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Cartridge
Particle Size
MS C18
MS C8
RP18
RP8
Phenyl
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
2.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
2.5 m
3.5 m
5 m
10 m
2.5 m
3.5 m
3.5 m
5 m
5 m
2.5 m
3.5 m
2.5 m
3.5 m
3.5 m
5 m
5 m
3.5 m
3.5 m
5 m
5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
186002198
186002199
186002200
186002210
186002211
186002212
186002469
186002470
186002471
186002599
186002600
186002601
186002602
186002603
186002604
186000979
186000386
186000390
186000394
1860006321
1860006481
186000900
186001908
186001907
186001906
186001921
186001923
1860006362
186001979
1860006522
186000592
186000398
186000594
186000400
1860004983
186000446
1860005383
186000404
1860005023
186000450
1860005423
186000408
1860005063
186000454
1860005463
186000458
1860005503
186000387
186000391
186000395
1860006331
1860006491
186001922
186001924
1860006372
186001980
1860006532
186000593
186000399
186000595
186000401
1860004993
186000447
1860005393
186000405
1860005033
186000451
1860005433
186000409
1860005073
186000455
1860005473
186000459
1860005513
186000388
186000392
186000396
1860006341
1860006501
186001925
1860006382
186001982
1860006542
186000402
1860005003
186000448
1860005403
186000406
1860005043
186000452
1860005443
186000410
1860005083
186000456
1860005483
186000460
1860005523
186000389
186000393
186000397
1860006351
1860006511
186001926
1860006392
186001983
1860006552
186000403
1860005013
186000449
1860005413
186000407
1860005053
186000453
1860005453
186000411
1860005093
186000457
1860005493
186000461
1860005533
186001188
186001192
186001189
186001193
Requires Guard Holder, Part No. WAT097958
Requires Cartridge Column Holder, Part No. 186000262
Requires Cartridge Column End Connector Kit, Part No. 700000117
186001179
186001185
186001180
186001186
186001181
186001187
Also available in Preparative Optimum Bed Density (OBD) Columns, See Page 189
www.waters.com
141
Dimension
Type
3.0 x 20 mm
3.0 x 20 mm
3.0 x 20 mm
3.0 x 20 mm
3.0 x 20 mm
3.0 x 20 mm
3.0 x 30 mm
3.0 x 30 mm
3.0 x 50 mm
3.0 x 50 mm
3.0 x 50 mm
3.0 x 50 mm
3.0 x 50 mm
3.0 x 100 mm
3.0 x 100 mm
3.0 x 100 mm
3.0 x 100 mm
3.0 x 150 mm
3.0 x 150 mm
3.0 x 150 mm
3.0 x 150 mm
3.0 x 250 mm
3.0 x 250 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 50 mm
3.9 x 50 mm
3.9 x 100 mm
3.9 x 100 mm
3.9 x 100 mm
3.9 x 150 mm
3.9 x 150 mm
3.9 x 150 mm
4.6 x 10 mm
4.6 x 10 mm
4.6 x 20 mm
4.6 x 20 mm
4.6 x 20 mm
4.6 x 20 mm
4.6 x 30 mm
4.6 x 30 mm
4.6 x 30 mm
4.6 x 50 mm
4.6 x 50 mm
4.6 x 50 mm
4.6 x 50 mm
4.6 x 50 mm
4.6x 100 mm
4.6 x 100 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 150 mm
4.6 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
4.6 x 250 mm
4.6 x 250 mm
IS Column
Cartridge
IS Column
Guard
IS Column
Guard
Column
Column
Column
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Cartridge
IS Column
IS Column
Guard
IS Column
Guard
Cartridge
Cartridge
Column
Cartridge
Column
Column
Column
Cartridge
Guard
Guard
IS Column
Cartridge
IS Column
IS Column
Column
Column
Column
Column
Column
Cartridge
Column
Cartridge
Column
Cartridge
Column
Column
Cartridge
Column
Cartridge
Column
Column
Cartridge
4
5
Particle Size
MS C18
MS C8
RP18
RP8
Phenyl
2.5 m
2.5 m
3.5 m
3.5 m
5 m
5 m
2.5 m
3.5 m
2.5 m
3.5 m
3.5 m
5 m
5 m
3.5 m
3.5 m
5 m
5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
2.5 m
3.5 m
3.5 m
5 m
5 m
3.5 m
5 m
3.5 m
3.5 m
5 m
3.5 m
5 m
5 m
3.5 m
5 m
2.5 m
2.5 m
3.5 m
5 m
2.5 m
3.5 m
5 m
2.5 m
3.5 m
3.5 m
5 m
5 m
3.5 m
3.5 m
5 m
3.5 m
3.5 m
5 m
5 m
3.5 m
5 m
5 m
186001972
1860005884
186001974
1860006404
186001984
1860006564
186000596
186000412
186000598
186000414
1860005105
186000462
1860005545
186000418
1860005145
186000466
1860005585
186000422
1860005185
186000470
1860005625
186000474
1860005665
186001899
186001900
1860006444
186001988
1860006604
1860008175
1860008155
186000426
1860005225
186000478
1860005705
1860019276
1860019206
186001889
1860005904
186001891
186001992
186000600
186000430
186000878
186000602
186000432
1860005265
186000482
1860005745
186000436
1860005305
186000486
186000440
1860005345
186000490
1860005785
186001470
186000494
1860005825
186001973
1860005894
186001975
1860006414
186001985
1860006574
186000597
186000413
186000599
186000415
1860005115
186000463
1860005555
186000419
1860005155
186000467
1860005595
186000423
1860005195
186000471
1860005635
186000475
1860005675
186001897
186001898
1860006454
186001989
1860006614
1860008185
1860008165
186000427
1860005235
186000479
1860005715
1860014346
186001890
1860005914
186001892
186001993
186000601
186000431
186000879
186000603
186000433
1860005275
186000483
1860005755
186000437
1860005315
186000487
186000441
1860005355
186000491
1860005795
186001471
186000495
1860005835
186001976
1860006424
186001986
1860006584
186000416
1860005125
186000464
1860005565
186000420
1860005165
186000468
1860005605
186000424
1860005205
186000472
1860005645
186000476
1860005685
186001902
1860006464
186001990
1860006624
186000428
1860005245
186000480
1860005725
186001893
186001994
186001910
186001909
186000434
1860005285
186000484
1860005765
186000438
1860005325
186000488
186000442
1860005365
186000492
1860005805
186001472
186000496
1860005845
186001977
1860006434
186001987
1860006594
186000417
1860005135
186000465
1860005575
186000421
1860005175
186000469
1860005615
186000425
1860005215
186000473
1860005655
186000477
1860005695
186001901
1860006474
186001991
1860006634
186000429
1860005255
186000481
1860005735
186001894
186001995
186001912
186001911
186000435
1860005295
186000485
1860005775
186000439
1860005335
186000489
186000443
1860005375
186000493
1860005815
186001473
186000497
1860005855
186001190
186001194
186001141
186001148
186001142
186001149
186001143
186001150
186001151
186001191
186001195
186001204
186001203
186001177
186001183
186001178
186001184
186001144
186001139
186001145
186001140
186001146
186001474
186001147
Requires Part No. WAT046910 (universal, use with standard columns) or Part No. WAT046905 (integrated into cartridge column)
Requires End Connector Kit, Part No. WAT037525 6 Requires Inline Guard Cartridge Holder, Part No. PSS830008
142
www.waters.com
XTerra Oligonucleotide Purification Columns
Dimension
4.6 x 50 mm
10 x 50 mm
Type
Particle Size
MS C18
Column
Column
2.5 m
2.5 m
186000602
186000982
XTerra Method Validation Kits

Dimension
2.1 x 100 mm
2.1 x 150 mm
3.9 x 150 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
Type
Particle Size
MS C18
MS C8
RP18
RP8
Phenyl
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
MV Kit
3.5 m
5 m
5 m
3.5 m
3.5 m
5 m
5 m
Custom
186000827
186000828
Custom
186000826
186000829
186000830
186000831
Custom
Custom
186000832
Custom
Custom
186000833
Custom
Custom
Custom
Custom
186000861
186000862
186000863
186000834
Custom
186000836
186000835
Custom
Custom
Custom
Custom
Custom
Custom
Custom
186002234
186002235
186002236
All other Method Validation kits are custom made on request.
Also available in Preparative Optimum Bed Density (OBD) Columns, See Page 189
Literature
References
XTerra Columns Brochure,
XTerra Applications Notebook,



www.waters.com
Description
Part No.

715001531
715002531
143
Symmetry Columns
Setting the Standard for Reproducibility
As todays c hemists establish new analytical methods for the latest
pharmaceutical and biopharmaceutical products, the selection of a
reproducible HPLC column is essential. T he selected column needs to
provide the same c hromatographic results over the life of the new
drug product. Symmetry columns demonstrate superior reproducibility
over many years. Batc hes randomly selected over a five year period
demonstrate excellent reproducibility in the example shown.
Reproducibility is our number one priority in supplying Symmetry columns.
T his excellent reproducibility is a result of our commitment to maintaining the tightest specifications in the HPLC column industry. Symmetry
columns are engineered with high purity raw materials, tightly controlled
manufacturing processes and column packing procedures that provide
todays scientists with the best, most reproducible HPLC column available.
Unmatched Year-to-Year Reproducibility

Column:
Symmetry C18,
4.6 x 150 mm, 5 m
WAT045905
H2O
MeCN
pH 3.75; 100 mM
NH4COOH in H2O
1.4 mL/min
30% A; 60% B; 10% C
5.0 L
30 C
UV @ 233 nm
Waters Alliance HT 2795
Waters 996 PDA detector
Sampling Rate: 5 pts./sec
Part No.:
Mobile Phase A:
Mobile Phase B:
Mobile Phase C:
Flow Rate:
Isocratic:
Injection Volume:
Temperature:
Detection:
Instrumentation:
RSDs for Retention Times

1. Terbinafine HCl
2. Ibuprofen
3. Lovastatin
4. Simvastatin
0.7%
0.8%
0.6%
0.7%
2
3
Our goal is to supply a family of HPLC columns that you can rely on for
rugged and robust methods. Symmetry columns let you increase your
laboratorys productivity, and allow easier method transfer between labs
and around the globe.
Batch 136 (1998)

Batch 142 (1998)
Batch 149 (1999)
Chemistry
Particle
Size
Particle
Shape
Pore
Size
Carbon
Load
Endcapped
Symmetry
C18
C8
3.5, 5 m
3.5, 5 m
Spherical
Spherical
100
100
19%
12%
Yes
Yes
SymmetryPrep
C18
C8
7 m
7 m
Spherical
Spherical
100
100
19%
12%
Yes
Yes
3.5, 5, 7 m Spherical
3.5, 5, 7 m Spherical
100
100
17%
15%
Yes
Yes
300
300
8.5%
2.8%
Yes
Yes
Packing
SymmetryShield
RP18
RP8
Symmetry300
C18
C4
144
3.5, 5 m
3.5, 5 m
Spherical
Spherical
Symmetry Analytical Columns
Batch 151 (2000)

Batch 156 (2001)
0
1.5
3.0
4.5
6.0
7.5
9.0
10.5
12.0
13.5
15.0 Min.
www.waters.com
T he Benefit of Narrow Specification Ranges
Symmetry columns have the narrowest ligand surface coverage specifications in the industry. Surface coverage (ligand density) is one of the most
important parameters affecting reproducibility. T he impact of a small
variation in surface coverage on the amitriptyline/acenaphthene alpha
specification for Symmetry C 18 columns is shown here. Narrow column
specifications benefit the c hromatographer in ac hieving reproducible
results. T he surface coverage requirements of Symmetry are the tightest
specifications in the industry, resulting in minimal shifts in resolution
and more reproducible batch-to-batch and column-to-column results.
C18 Ligand Surface Coverage Specification Ranges

3.09
Symmetry C18
3.31
3.75
2.75
Column X
2.6
no upper limit
Column Y
1.5
2.0
2.5
3.0
3.5
4.0
C18 Surface Coverage (mol/m2)
Narrow Specification Ranges Provide Smaller Shifts in Alpha (a)
Minimal Changes in Resolution Means More Reproducible Results*
Alpha for Amitriptyline/Acenaphthene
2
1.8
4.14
Symmetry C18
1.6
4.14
2.86
1.4
4.14
2.86
1.2
Specification Ranges
for Symmetry C18
Specification X
0.8
1.5
2.0
2.5
3.0
3.5
4.0
C18 Surface Coverage (mol/m2)
Smaller Shifts in Alpha (a) Reduce Changes in Peak Resolution

R=
2.86
N
4
)( a-1a )( KK+1 )
2
Specification Y
R = resolution
N = number of peaks
a = alpha value
k = peak retention
4.70
0.81
4.70
0.81
4.70
0.81
5.37
5.37
5.37
Maximum Resolution
Minimum Resolution
* Based on vendors column product specifications for ligand density
Long Lifetime
Outstanding Packed Bed Stability10,000 Injections
Columns that last longer save you money. Symmetry columns can last over
10,000 injections with minimal loss in efficiency, minimal increase in
backpressure and minimal change in retention time.
Column:
Symmetry C8, 3.9 mm x 150 mm, 5 m

with Sentry Guard Column
3.9 mm x 20 mm
Water/methanol/glacial
acetic acid 79:20:1
1.0 mL/minute
10 L
25 C
UV @ 254
1. Sulfanilamide
2. Sulfadiazine
3. Sulfathiazole
4. Sulfamerazine
5. Sulfamethazine
6. Succinylsulfathiazole
Mobile Phase:
T hese columns deliver guaranteed consistent performance optimizing the

two key factors that control column lifetime which are hydrolytic stability of
the packing material and mechanical stability of the packed bed.
Flow Rate:
Injection Volume:
Temperature:
Detector:
Analytes:
Comprehensive Certificate of Analysis
Injection 10,000
Start
0
A three-page Certificate of Analysis is included with every Symmetry

column. We report both our specifications, which are the tightest in the
industry, and the results of the 22 critical tests each column must pass
before it carries the Symmetry brand.
www.waters.com
10 Min.
The long-term mechanical stability of Symmetry columns is demonstrated in this analysis of sulfa drugs.
Over 10,000 injections were run with minimal loss in column efficiency. The use of the Sentry guard
column is critical to achieving these types of column lifetimes.
145
Symmetry Columns and Cartridges
Dimension
Symmetry IS Columns
Particle Size
C18
C8
1.0 x 50 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 50 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 30 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
WAT106056
WAT248059
WAT058973
WAT200650
WAT058965
WAT106005
186002612
186000696
186000695
186000271
WAT200625
WAT066224
WAT066220
WAT200632
WAT106052
WAT248072
WAT058977
WAT200624
WAT058961
WAT106011
186002613
186000698
186000697
186000270
WAT200620
WAT066200
WAT066204
WAT200630
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 150 mm
3.0 x 250 mm
3.9 x 150 mm
4.6 x 50 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186000206
186002608
WAT056975
WAT054200
186000690
WAT046980
186000207
186002616
WAT045905
WAT054275
186000212
186002609
WAT056955
WAT054230
186000691
WAT046970
186000213
186002617
WAT045995
WAT054270
Dimension
Particle Size
C18
C8
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
4.6 x 20 mm
3.5 m
3.5 m
3.5 m
3.5 m
186002066
186002074
186002082
186002090
186002067
186002075
186002083
186002091
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
4.6 x 20 mm
5 m
5 m
5 m
5 m
186002070
186002078
186002086
186002094
186002071
186002079
186002087
186002095
Symmetry Sentry Guard Columns (2/pk)

(Requires Sentry Guard Holders, see page 165)
Dimension
Particle Size
C18
C8
3.5 m
5 m
WAT106127
WAT054225
WAT106128
WAT054250
2.1 x 10 mm
3.9 x 20 mm
Symmetry Column and Cartridge Column Method Validation Kits

T hree columns from three different batches to test reproducibility.
Dimension
Symmetry Cartridge Columns

(Requires endfittings, see page 165)
Dimension
Particle Size
C18
C8
2.1 x 20 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
4.6 x 75 mm
4.6 x 100 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
186000269
186000152
186000151
186000150
WAT066260
WAT066265
186000268
186000149
186000153
186000148
WAT066210
WAT066215
3.9 x 50 mm
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
5 m
5 m
5 m
5 m
WAT054220
WAT054205
WAT054210
WAT054215
WAT054240
WAT054235
WAT054255
WAT054245
Type
Particle Size
C18
C8
4.6 x 150 mm
2.1 x 150 mm
3.0 x 150 mm
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
Column
Column
Column
Column
Column
Column
3.5 m
5 m
5 m
5 m
5 m
5 m
WAT094240
WAT094234
WAT054446
WAT047210
WAT054448
WAT054450
WAT094237
WAT094231
WAT054434
WAT046955
WAT054435
WAT054438
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
Cartridge*
Cartridge*
Cartridge*
5 m
5 m
5 m
WAT054452
WAT054454
WAT054456
WAT054440
WAT054442
WAT054444
* Requires endfittings, see page 165
Literature
References
Symmetry Family of Columns Brochure,
Symmetry Family of Columns
Applications Notebook,
146
www.waters.com
SymmetryShield Columns
Excellent Peak Shape
Industry Leader for Peak Shape of Basic Compounds
SymmetryShield HPLC columns are an excellent choice to achieve superior

peak shapes for basic compounds. T hese reversed-phase columns are based
on Waters patented embedded polar group tec hnology t hat literally
shields the silicas residual surface silanols from highly basic analytes.
SymmetryShield RP18
Brand L
1.66
Brand HE
1.75
Brand DO
1.78
Brand DA
1.9
Polar
Group
Si
1.5
Brand HP
Shield Technology
In this comparison test,

SymmetryShield RP18
has the lowest USP tailing
factor for the basic probe
amitriptyline under the most
demanding pH 7 mobilephase conditions.
1.2
1.95
Brand I
2.39
Brand P
3.6
Brand E
CH3
CH3
Reduce interaction with silanols

Improve water-wettability
T he embedded polar group, which is in close proximity to the silica surface,

further reduces the activity of the surface silanols in SymmetryShield
packing materials.
a: SymmetryShield RP18
1
Flow Rate:
Detection:
Temperature:
Analytes:
6
7
10
b: Symmetry C18
In the example shown, a leading pharmaceutical company was faced with

a challenging separation. It was only when a SymmetryShield column was
substituted for a conventional C8 column that a much better separation was
achieved without having to change mobile-phase conditions.
SymmetryShield columns with Shield Technology are the perfect choice for
applications that require low organic, highly aqueous mobile-phase conditions. T hese reversed-phase particles are water-wettable allowing better
interaction between the particles and your sample. T he result is stable
retention in highly aqueous mobile phases with superior peak shape.
www.waters.com
USP Tailing Factor = 1.2
Obtaining a good separation is paramount. SymmetryShield columns exhibit

less retention for basic compounds due to lower silanol activity than
conventional C 18 and C 8 columns. SymmetryS hield columns are an
excellent methods development tool.
15
20
25
30 Min.
2
3
AU
Perfect for Use with Highly

Aqueous Mobile Phases
a. SymmetryShield RP18
3.9 x 150 mm
b. Leading Brand C18
65% methanol; 35% 20 mM
KH2PO4/K2HPO4 at pH 7
1.0 mL/min
UV @ 254 nm
23 C
1. Uracil
2. Propranolol
3. Butylparaben
4. Dipropylphthalate
5. Naphthalene
6. Amitriptyline
7. Acenaphthene
Mobile Phase:
Exhibit less retention of basic compounds than

conventional C18 and C8 columns.
Unique Selectivity
Column:
Deliver significantly improved peak shape

and resolution over a broad pH range
In fact, SymmetryShield columns deliver USP Tailing Factors of less

than 1.2 over a pH range between pH 2 and pH 8. By delivering lower
limits of detection and quantitation, SymmetryShield columns will
enable you to develop new methods faster and improve the productivity
of your laboratory.
SymmetryShield Columns Deliver Unique Selectivity
AU
2
USP Tailing Factor
As a result, SymmetryShield columns:
USP Tailing Factor = 1.9
10
15
20
SymmetryShield Analytical Columns
25
30 Min.
147
SymmetryShield ColumnsNo Pore Dewetting in Highly Aqueous Mobile Phases
SymmetryShield RP8 ColumnsNo Pore Dewetting!
Standard Silica C8 ColumnsPore Dewetting

Mobile Phase: Aqueous
0.1% Acetic Acid
Column:
Amoxicillin
10 Min.
Column:
Amoxicillin
Most HPLC columns lose their retention

when running low or 0% organic mobile
phases if the flow is stopped. When this
happens the bonded particle pores become
de-wetted. The result is termed pore
dewetting and you lose the rententivity
of your analyte.
Initial
After pump stopped and re-started

(Pore dewetting: ~ 100%)
0
Mobile Phase: Aqueous

0.1% Acetic Acid
Standard C8 Silica Column

4.6 x 150 mm, 5 m
SymmetryShield columns prevent this

from happening because the embedded
polar group maintains the wetted pore
surface, ensuring retention of your
analyte and eliminating the need
for a lengthy re-wetting and column
re-equilibration process.
Initial
No change in
retention time
0
After pump stopped

and re-started
(Pore dewetting: ~ 3%)
4
SymmetryShield RP8
4.6 x 150 mm, 5 m
10 Min.
No retention for analyte
Dimension
SymmetryShield IS Columns
Particle Size
RP18
RP8
1.0 x 50 mm
1.0 x 150 mm
2.1 x 30 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 50 mm
3.0 x 100 mm
3.0 x 150 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
186000175
186000176
186000171
186000172
186000173
186000174
186002614
186000700
186000699
186000177
186000178
186000179
186000180
WAT106060
WAT106048
WAT106001
WAT094257
WAT058969
WAT106008
186002615
186000703
186000702
WAT094260
WAT094263
WAT094266
WAT094269
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
3.0 x 150 mm
3.0 x 250 mm
3.9 x 150 mm
4.6 x 50 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186000217
186000998
186000111
186000692
186000693
186000108
186000218
186002618
186000109
186000112
186000223
186002611
WAT094245
WAT094243
186000694
WAT200655
186000224
186002619
WAT200662
WAT200670
Dimension
Particle Size
RP18
RP8
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
4.6 x 20 mm
3.5 m
3.5 m
3.5 m
3.5 m
186002068
186002076
186002084
186002092
186002069
186002077
186002085
186002093
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
4.6 x 20 mm
5 m
5 m
5 m
5 m
186002072
186002080
186002088
186002096
186002073
186002081
186002089
186002097
SymmetryShield Sentry Guard Columns (2/pk)

Dimension
Particle Size
RP18
RP8
3.5 m
3.5 m
5 m
186000169
186000701
186000107
WAT106129
186000704
WAT200675
2.1 x 10 mm
3.9 x 20 mm
3.9 x 20 mm
SymmetryShield Column and Cartridge

Column Method Validation Kits
SymmetryShield Cartridge Columns
Dimension

Dimension
Particle Size
RP18
RP8
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
4.6 x 75 mm
4.6 x 100 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
186000168
186000167
186000166
186000183
186000170
186000147
186000146
186000145
WAT094272
WAT094275
3.9 x 50 mm
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
5 m
5 m
5 m
5 m
186000106
186000110
186000113
WAT094248
WAT200658
WAT200665
WAT200661
148
SymmetryShield Analytical Columns
Type
Particle Size
RP18
RP8
2.1 x 150 mm
4.6 x 150 mm
2.1 x 150 mm
3.0 x 150 mm
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
Column
Column
Column
Column
Column
Column
Column
3.5 m
3.5 m
5 m
5 m
5 m
5 m
5 m
186000182
186000181
186000100
186000104
186000103
186000102
WAT094278
WAT094254
WAT094251
WAT210594
WAT210588
WAT210591
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
Cartridge*
Cartridge*
Cartridge*
5 m
5 m
5 m
186000105
186000101
186000114
WAT210582
WAT210585
WAT210579
www.waters.com
Symmetry300 Columns
High Recoveries of Proteins and Peptides
Symmetry300 columns are wide-pore, reversed-phase columns whic h
are built on t he Symmetry column platform, t he standard t hat t he
pharmaceutical industry has come to rely on for sensitive, rugged and
robust HPLC analyses.
Peptide Separation at High Mass Load (0.1% TFA)
Columns:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
4.6 x 50 mm, 5 m
0.1% TFA in H2O
0.08% TFA in ACN
0.75 ml/min
Time
Profile
(min)
%A
%B
0.00
100
0
25.00 50
50
Temperature:
Detection:
Instrument:
Symmetry300 C18,
4.6 x 50 mm column
P = 98.8*
2
1
40 C
UV 220 nm
7
4
5 6
10
15
20 Min.
Biologically Active Peptide Mixture1

Name
1. L2275
2. A6677
3. Bradykinin
4. Angiotensin II
5. Angiotensin I
6. Substance P
7. Renin substrate
8. Insulin B chain
9. Melittin
MW
986.6
1001.5
1060.2
1046.2
1296.5
1347.6
1759.0
3495.9
2845.8
pI
12.04
9.34
12.00
7.35
7.51
11.20
7.61
7.51
12.06
Hydrophobicity Number of
(Meek)
Amino Acids
2.8
9
3.0
10
42.6
9
43.7
8
54.0
10
52.7
11
63.5
14
129.4
30
92.3
26
J. L. Meek and Z. L. Rossetti, J. Chromatogr., 211 (1981) 15-28.
pI = Isoelectric pH
Peptide Separation at Low Mass Load (0.1% Formic Acid)

Columns:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Column-after-column, no matter who uses your methods or where they are

used around the world, the Symmetry300 columns are the best solution for
validation compliance.
Sharpest Peaks for Highest Resolution
Symmetry300 columns perform better at high and low mass load

conditions. For mass spectrometry applications, we recommend low
T FA mobile phases or 0.1% formic acid.
Symmetry300 columns are available in two particle sizes: 3.5 m and

5 m, and in two chemistries: C4 for large peptides and proteins, and C18
for smaller peptides.
Leading 300 C18 Brand,

4.6 x 50 mm
P = 70.0*
Symmetry300 C18 columns provide better peak shape and peak capacity
compared to other leading brands when separating biologically active
peptides. In the examples shown, Symmetry300 columns demonstrate
superior performance when simultaneously separating hydrophilic and
hydrophobic peptides, as well as neutral and highly basic peptides. Also,
hydrophobicity (Meek retention prediction 1) is included. T he lower
hydrophobicity values indicate the peptide is more hydrophilic.
4.6 x 50 mm, 5 m
0.1% formic acid in H2O
0.08% formic acid in ACN
0.75 ml/min
Gradient:
Injection Volume:
Temperature:
Detection:
Instrument:
6
3
Time
Profile
(min)
%A
%B
0
100
0
25
50
50
20 L
40 C
UV 220 nm
Symmetry300 C18 ,
4.6 x 50 mm
P = 93.1*
T he heart of the column is the high purity base deactivated silica. T he silica
used in Symmetry300 columns is synthesized using ultrapure organic
reagents, resulting in high purity with very low silanol activity.
In addition, when combined with the high surface coverage of the bonded
phase the result is higher recoveries of proteins and peptides even when
injected at low concentrations.
Symmetry300 columns deliver the sharpest peaks for biomolecules and
deliver more peak capacity for complex tryptic maps. T hey are packed to
the highest efficiency and reproducibility of any product on the market.
T his results in improved precision for impurity and stability assays, more
accurate quantitation, and adaptability to more rugged methods.
Unsurpassed Reproducibility
Cytochrome c Tryptic Map Q.C. Test
Column:
Waters batch release protocol includes a

tryptic map of cytochrome c which is used to
test for reproducibility of retention time and
resolution. In this test, the tryptic map was
run on four Symmetry300 columns, each
containing a different batch of packing.
Part Number:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
Symmetry300 C18
3.9 x 150 mm, 5 m
WAT106154
0.05% TFA in water
1.0 mL/min
Time
Profile
(min)
%A
%B
0
100
0
45
72
28
20 L
35 C
UV @ 220 nm
Injection Volume:
Temperature:
Detection:
Leading 300 C18 Brand,

4.6 x 50 mm
P = 62.3*
Batch
Batch107
107
Batch
106
Batch
106
10
15
Batch
104
Batch
104
20 Min.
Batch
103
Batch
103
* Note: Peak Capacity (P) is defined as the number of theoretical peaks that can
fit into a chromatographic run within a certain gradient time.
10
30
50 Min.
P = 1 + (tg/w5s) Where tg = gradient time w5s = peak measured at 4.4% of peak height
www.waters.com
Symmetry300 Analytical Columns
149
Excellent Protein Separations
Protein: Symmetry300 C4 Versus Competitors
Symmetry300 C4 columns have the highest average peak capacity when

compared to over 20 leading brands of HPLC columns. In the example
s hown, w hic h is considered to be a difficult protein separation, t he
Symmetry300 C4 column provided a better separation with better peak
shape compared to a leading competitive 300 column.
Columns:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
4.6 x 50 mm, 5 m
0.1% TFA in water
0.75 mL/min
Time
Profile
(min)
%A
%B
0.0
80
20
15.0
35
65
Injection Volume:
Temperature:
Detection:
Instrument:
Analytes:
20 L
40 C
UV @ 220 nm
Waters Alliance 2790, 996 PDA
1. RNAse
2. BSA
3. B-Lactoglobulin
4. Ovalbumin
Symmetry300 C4
P = 138.56
Competitive 300 C18

P = 90.09
Particle Size
C18
C4
1.0 x 150 mm
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
4.6 x 50 mm
4.6 x 75 mm
4.6 x 100 mm
4.6 x 150 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
186000185
186000187
186000188
186000200
186000201
186000189
186000190
186000197
186000276
186000277
186000278
186000279
186000280
186000281
186000282
186000283
2.1 x 150 mm
3.9 x 150 mm
4.6 x 50 mm
4.6 x 150 mm
4.6 x 250 mm
5 m
5 m
5 m
5 m
5 m
WAT106172
WAT106154
WAT106209
WAT106157
WAT106151
186000285
186000286
186000287
186000288
186000289
Symmetry300 Cartridge Columns

Dimension
Particle Size
C18
2.1 x 50 mm
4.6 x 75 mm
3.5 m
3.5 m
186000199
186000192
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
5 m
5 m
5 m
WAT106169
WAT106163
WAT106160
150
10
12
14 Min.
Symmetry300 Sentry Guard Columns (2/pk)

Dimension

Dimension
Particle Size
C18
C4
3.5 m
5 m
186000198
WAT106166
186000275
186000284
2.1 x 10 mm
3.9 x 20 mm
Symmetry300 Column and Cartridge

Column Method Validation Kits
Dimension
Type
Particle Size
C18
C4
2.1 x 150 mm
4.6 x 150 mm
Column
Column
3.5 m
3.5 m
186000194
186000195
186000290
186000291
2.1 x 150 mm
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
Column
Column
Column
Column
5 m
5 m
5 m
5 m
WAT106193
WAT106187
WAT106190
WAT106184
186000292
186000293
186000294
186000295
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
Cartridge*
Cartridge*
Cartridge*
5 m
5 m
5 m
WAT106181
WAT106175
WAT106178
www.waters.com
Symmetry NanoEase Nano and Capillary Columns
NanoEase columns contain high performance, small particle sorbents.
Exact manufacturing and QC production procedures ensure consistent
column performance. Superior column longevity is ensured by a novel
frit tec hnology that minimizes bed disturbance throughout the life of
the column.
Superior c hromatographic performance
Available in variety of Waters stationary phases
Robust and easy to handle
Can be used with any capillary LC system
NanoEase LC/MS Nano Column Reproducibility
Description
500 fmol Enolase Digest

Column:
Eluent A:
Eluent B:
NanoEase Symmetry C18,

3.5 m, 75 m x 100 mm
0.1% FA in water
0.1% FA, 2% water in acetonitrile
Gradient:
Flow Rate:
System:
5-50% B in 60 min
400 nL/min
Waters CapLC operated in
split flow mode, Q-Tof micro
100
TOF MS ES+
TIC
9.78e4
Inj #4
100
%
Inj #3
TOF MS ES+
TIC
1.48e5
Inj #2
TOF MS ES+
TIC
1.71e5
100
%
0
100
TOF MS ES+
TIC
1.69e5
Inj #1
%
0
10
www.waters.com
20
30
40
50
60 Min.
Column i.d.
Length
Particle Size
Part No.
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
75 m
75 m
75 m
75 m
75 m
75 m
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
186002188
186002189
186002190
186002191
186002192
186002193
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry300 C18
100 m
100 m
100 m
100 m
50 mm
100 mm
150 mm
150 mm
3.5 m
3.5 m
3.5 m
3.5 m
186002201
186002202
186002203
186002206
Symmetry C18
Symmetry C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
150 m
150 m
150 m
150 m
150 m
50 mm
150 mm
50 mm
100 mm
150 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
186002459
186002461
186002462
186002463
186002464
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
186002581
186002582
186002583
186002584
186002585
186002586
186002587
186002588
186002589
186002590
186002591
186002592
Symmetry NanoEase Nano and Capillary Columns
151
Waters Spherisorb Columns

One of the great advantages of c hoosing Waters Spherisorb columns
is the range of c hoices available to you. Waters Spherisorb columns
are produced in a wide range of sizes and bonded phases to meet your
chromatographic needs. Silica is produced in 3, 5 and 10 micron particle
sizes. T he high quality bonded phases give many different and unique
separation selectivities.
Particle
Shape
Surface
Area
Pore
Size
Pore
Volume
Particle
Size
Carbon
Load
Ligand
Coverage
Endcapped
Waters Spherisorb ODS2 (C18)

Waters Spherisorb ODS1 (C18)
Waters Spherisorb ODSB (C18)
Spherical
Spherical
Spherical
220 m2/g
220 m2/g
220 m2/g
80
80
80
0.50 ml/g
0.50 ml/g
0.50 ml/g
3, 5, 10 m
3, 5, 10 m
5 m
11.5%
6.2%
11.5%
2.98 Mol/m2
1.49 Mol/m2
2.98 Mol/m2
yes
no
yes
Spherical
Spherical
Spherical
220 m2/g
220 m2/g
220 m2/g
80
80
80
0.50 ml/g
0.50 ml/g
0.50 ml/g
3, 5, 10 m
3, 5, 10 m
3, 5, 10 m
5.75%
4.7%
2.15%
3.12 Mol/m2
3.36 Mol/m2
2.97 Mol/m2
yes
yes
no
Waters Spherisorb NH2 (Amino)

Waters Spherisorb Phenyl
Waters Spherisorb CN (Nitrile)
Spherical
Spherical
Spherical
220 m2/g
220 m2/g
220 m2/g
80
80
80
0.50 ml/g
0.50 ml/g
0.50 ml/g
3, 5, 10 m
3, 5, 10 m
3, 5, 10 m
1.9%
2.5%
3.1%
2.64 Mol/m2
1.72 Mol/m2
3.29 Mol/m2
no
no
no
Waters Spherisorb OD/CN

Waters Spherisorb W (Silica)
Spherical
Spherical
220 m2/g
220 m2/g
80
80
0.50 ml/g
0.50 ml/g
5 m
3, 5, 10 m
5%
1.15 Mol/m2
yes
Spherical
Spherical
220 m2/g
220 m2/g
80
80
0.50 ml/g
0.50 ml/g
5, 10 m
5, 10 m
4%
4%
no
no
Product Description
Waters Spherisorb Phases
Waters Spherisorb Ion Exchangers

Waters Spherisorb SCX
Waters Spherisorb SAX
Waters Spherisorb Analytical Columns

Waters Spherisorb analytical columns come with industry standard endfittings. T he threaded female fitting requires a Parker-style compression
screw and ferrule to connect the HPLC tubing to the analytical column. If
your instrument is set up for Waters columns, replace your ferrule on the
connector tubing and set it for the Parker standard.
Waters Spherisorb Cartridge Columns

Waters Spherisorb cartridge columns require reusable cartridge endfittings
(Part No. PSS614100). T hese endfittings should be ordered the first time a
cartridge is purchased. T he reusable endfittings are a Parker-style standard
endfitting. Coupler fittings are also available to allow you to link a guard
cartridge to your analytical cartridge.
152
Waters Spherisorb Columns (10 m packing)

Chemistry
S10 ODS2
S10 ODS1
S10 C8
S10 C6
S10 C1
S10 NH2
S10 P (Phenyl)
S10 CN
S10 W (Silica)
4.6 mm
x 100 mm
4.6 mm
x 150 mm
4.6 mm
x 200 mm
4.6 mm
x 250 mm
PSS830712
PSS833612
PSS833512
PSS830212
PSS832513
PSS830713
PSS832813
PSS833213
PSS833013
PSS833613
PSS833813
PSS833513
PSS830213
PSS832514
PSS830714
PSS832514
PSS830214
PSS832515
PSS830715
PSS832815
PSS833215
PSS833015
PSS833615
PSS833815
PSS833515
PSS830215
PSS833912
PSS833913
PSS837613
PSS833915
PSS837615
Ion Exchange
S10 SAX
S10 SCX
www.waters.com
Waters Spherisorb 5 m Columns and Cartridges
Columns
Chemistry
S5 ODS2
S5 ODS1
S5 ODS B
S5 C8
S5 C6
S5 C1
S5 NH2
S5 P (Phenyl)
S5 CN Normal Phase
S5 CN Reversed Phase
S5 W (Silica)
Cartridge Columns
4.0 mm
x 125 mm
4.0 mm
x 250 mm
4.6 mm
x 50 mm
4.6 mm
x 100 mm
4.6 mm
x 150 mm
4.6 mm
x 250 mm
4.6 mm
x 50 mm
4.6 mm
x 100 mm
4.6 mm
x 150 mm
4.6 mm
x 250 mm
PSS845543
PSS845541
PSS845280
PSS845284
PSS845288
PSS845300
PSS845292
PSS845296
PSS845539
PSS845277
PSS845542
PSS845281
PSS845285
PSS845289
PSS845301
PSS845293
PSS845297
PSS845540
PSS831911
PSS830611
PSS831811
PSS831011
PSS832611
PSS831111
PSS830911
PSS830111
PSS831912
PSS830612
PSS831812
PSS831012
PSS832612
PSS831112
PSS830812
PSS830912
PSS830112
PSS831913
PSS830613
PSS831813
PSS831013
PSS832613
PSS831113
PSS830813
PSS830913
PSS830908
PSS830113
PSS831915
PSS830615
PSS831815
PSS831015
PSS832615
PSS831115
PSS830815
PSS830915
PSS830909
PSS830115
PSS839536
PSS839506
PSS839531
PSS839521
PSS839566
PSS839526
PSS839511
PSS839516
PSS839537
PSS839507
PSS839532
PSS839522
PSS839567
PSS839527
PSS839512
PSS839517
PSS839502
PSS839538
PSS839508
PSS839613
PSS839533
PSS839523
PSS839568
PSS839528
PSS839513
PSS839518
PSS839503
PSS839540
PSS839510
PSS839615
PSS839535
PSS839525
PSS839570
PSS839530
PSS839515
PSS839520
PSS839505
PSS845304
PSS845308
PSS845305
PSS845309
PSS832711
PSS837511
PSS832712
PSS837512
PSS832713
PSS837513
PSS832715
PSS837515
PSS839571
PSS839651
PSS839572
PSS839652
PSS839573
PSS839653
PSS839575
PSS839655
PSS837812
PSS837813
PSS837815
PSS839643
PSS839645
Ion Exchange
S5 SAX
S5 SCX
Mixed Mode
S5 OD/CN
Waters Spherisorb 3 m Columns and Cartridges

Columns
Chemistry
S3 ODS2
S3 ODS1
S3 C8
S3 C6
S3 C1
S3 NH2
S3 P (Phenyl)
S3 CN Normal Phase
S3 W (Silica)
Cartridge Columns
4.6 mm
x 50 mm
4.6 mm
x 60 mm
4.6 mm
x 100 mm
4.6 mm
x 150 mm
4.6 mm
x 30 mm
4.6 mm
x 50 mm
4.6 mm
x 100 mm
4.6 mm
x 150 mm
PSS832111
PSS833411
PSS832211
PSS833111
PSS832911
PSS832311
PSS833711
PSS832411
PSS832011
PSS839853
PSS839852
PSS832112
PSS833412
PSS832212
PSS833112
PSS832912
PSS832312
PSS833712
PSS832412
PSS832012
PSS832113
PSS833413
PSS832213
PSS833113
PSS832913
PSS832313
PSS833713
PSS832413
PSS832013
PSS830065
PSS830068
PSS839546
PSS839551
PSS839581
PSS839576
PSS839556
PSS839591
PSS839561
PSS839541
PSS839547
PSS839587
PSS839552
PSS839582
PSS839577
PSS839557
PSS839592
PSS839562
PSS839542
PSS839548
PSS839588
PSS839553
PSS839583
PSS839578
PSS839558
PSS839593
PSS839563
PSS839543
Waters Spherisorb 5 m Cartridge Columns

Chemistry
S5 ODS2
S5 ODS1
S5 C8
S5 C6
S5 C1
S5 NH2
S5 P (Phenyl)
S5 CN Normal Phase
S5 CN Reversed Phase
S5 W (Silica)
Waters Spherisorb Semiprep Columns
3.0 mm
x 125 mm
3.0 mm
x 250 mm
4.0 mm
x 125 mm
4.0 mm
x 250 mm
PSS838529
PSS838530
PSS838533
PSS838535
PSS838531
PSS838534
PSS838522
PSS838523
PSS838525
PSS838526
PSS838524
PSS838521
PSS845553
PSS845551
PSS845555
PSS845557
PSS845559
PSS845565
PSS845561
PSS845563
PSS845549
PSS845554
PSS845552
PSS845556
PSS845558
PSS845560
PSS845566
PSS845562
PSS845564
PSS845550
PSS845567
PSS845569
PSS845568
PSS845570
Ion Exchange
S5 SAX
S5 SCX
www.waters.com
5 m
Chemistry
S5 ODS2
S5 ODS1
S5 C8
S5 C6
S5 C1
S5 NH2
S5P (Phenyl)
S5 CN
S5 W (Silica)
10 mm
x 250 mm
20 mm
x 250 mm
PSS831985
PSS830685
PSS831885
PSS831085
PSS832685
PSS831185
PSS830885
PSS830985
PSS830185
PSS831995
PSS830695
PSS831895
PSS831095
PSS832695
PSS831195
PSS830895
PSS830995
PSS830195
PSS832785
PSS837585
PSS832795
PSS837595
10 m
Chemistry
10 mm
x 250 mm
20 mm
x 250 mm
S10 ODS2
S10 ODS1
S10 C8
S10 C6
S10 C1
S10 NH2
S10 P (Phenyl)
S10 CN
S10 W (Silica)
PSS832585
PSS830785
PSS832885
PSS833285
PSS833085
PSS833685
PSS833885
PSS833585
PSS830285
PSS832595
PSS830795
PSS832895
PSS833295
PSS833095
PSS833695
PSS833895
PSS833595
PSS830295
S10 SAX
S10 SCX
PSS833985
PSS837685
PSS833995
PSS837695
Ion Exchange
S5 SAX
S5 SCX
153
Waters Spherisorb Microbore Columns
3 m
Chemistry
1 mm
x 100 mm
1 mm
x 150 mm
S3 ODS2
S3 ODS1
S3 C8
PSS832132
PSS832232
PSS832133
PSS833433
PSS832233
S3 NH2
S3 CN
S3 W (Silica)
PSS832432
PSS832032
PSS832333
PSS832433
PSS832033
5 m
Chemistry
S5 ODS2
S5 ODS1
S5 C8
S5 C6
S5 NH2
S5 CN
S5 W (Silica)
1 mm
x 100 mm
1 mm
x 150 mm
1 mm
x 250 mm
PSS831932
PSS830632
PSS831832
PSS831032
PSS831132
PSS830932
PSS831933
PSS831935
PSS830635
PSS831135
PSS830135
2 mm
x 100 mm
2 mm
x 150 mm
2 mm
x 250 mm
PSS831922
PSS830622
PSS831822
PSS831122
PSS830922
PSS830122
PSS831923
PSS830623
PSS831823
PSS831123
PSS830923
PSS830123
PSS831925
PSS830625
PSS831825
PSS831125
PSS830925
PSS830125
Waters Spherisorb Narrow-Bore Columns

3 m
Chemistry
S3 ODS2
S3 ODS1
S3 C8
S3 NH2
S3 CN
S3 W (Silica)
2 mm
x 100 mm
2 mm
x 150 mm
PSS832122
PSS833422
PSS832222
PSS832322
PSS832422
PSS832022
PSS832123
PSS833423
PSS832223
PSS832323
PSS832423
PSS832023
5 m
Chemistry
S5 ODS2
S5 ODS1
S5 C8
S5 NH2
S5 CN
S5 W (Silica)
Cartridge Fittings and Accessories

T hese endfittings and accessories can be used with 4.6 mm, 4.0 mm, or
3.0 mm i.d. cartridges.
Description
Qty.
Part No.
Removable Column Endfitting

Frit Assembly (2 m)
Frit Assembly (0.5 m)
Column Coupler
Long Tail Endfitting
Extended Endfitting for use with 10 mm Integral Guard
Nylon Column Plugs for Storage of Complete Column
Nylon Column Caps for Storage of
Replacement Cartridge Column
Inline 10 mm Guard Cartridge Holder Kit
for use with above items
2/pk
5/pk
5/pk
2/pk
2/pk
1/pk
1/pk
PSS614100
PSS614103
PSS614104
PSS614102
PSS614101
PSS614108
WAT015674
10/pk
PSS614113
PSS830008
1. 30 mm Stand Alone Guard/Column (endfittings not included)

2. Extended endfitting for use with 10 mm Integral Guard: PSS614108
3. 10 mm Integral Guard Column
4. Column Coupler: PSS614102
Inline Guard Cartridge Holder
Waters Spherisorb Guard Cartridges
10 mm Guard Cartridge (4.6 mm i.d.)
Chemistry
10 x 4.6 mm
3/pk
30 x 4.6 mm
Guard 3/pk
S5 ODS2
S5 ODS1
S5 C8
S5 C6
S5 C1
S5 CN
S5 Phenyl
S5 NH2
S5 W Silica
S5 SAX
S5 SCX
S5 ODSB
PSS830053
PSS830073
PSS830074
PSS830075
PSS830076
PSS830077
PSS830078
PSS830079
PSS830051
PSS830055
PSS830057
PSS830059
PSS839458
PSS839472
PSS839473
PSS839474
PSS839475
PSS839476
PSS839477
PSS839478
PSS839451
PSS839465
PSS839471
PSS839470
154
Inline Guard Cartridge Holder

PSS 830008
Extended Endfitting for use with 10 mm Guard Cartridges

Waters Spherisorb
Cartridge
10 mm Guard Cartridge (4.6 mm i.d.)
Extended Endfitting
for use with 10 mm Guard
PSS 614108
www.waters.com
Nova-Pak Packings
T he 4 m small particle size of Nova-Pak bonded phases offers high
resolution as well as more efficient and faster c hromatography. T his
is accomplished by using shorter columns, thereby reducing solvent
consumption or by using longer columns to resolve even the most
complex mixture. Analytical columns with 4 m particle size packings
are available in 75, 150, and 300 mm length steel columns. Steel
cartridge columns with reusable endfittings are available in 50, 100,
150, and 250 mm lengths. Semi-preparative columns with 6 micron
particle size packings give you an unparalleled range of separation
possibilities. Stringent QC procedures in our cGMP manufacturing facility
ensure batc h-to-batc h reproducibility.
Rapid Analysis of Soft Drink Additives and Artificial
Sweeteners on Nova-Pak C18 Column
Column:
Part Number:
Mobile Phase:
Flow Rate:
Detection:
Sample:
Packing
Chemistry
Particle
Size
Particle
Shape
Pore
Size
Carbon
Load
Endcapped
Nova-Pak
C18
C8
Phenyl
CN HP
Silica
4 m
4 m
4 m
4 m
4 m
Spherical
Spherical
Spherical
Spherical
Spherical
60
60
60
60
60
7%
4%
5%
2%
N/A
Yes
Yes
Yes
Yes
N/A
Prep Nova-Pak HR
C18
Silica
6 m
6 m
Spherical
Spherical
60
60
7%
N/A
Yes
N/A
Anticonvulsants
Waters Nova-Pak C18,

3.9 x 150 mm
WAT086344
Acetonitrile: Ammonium Acetate
with 2.5% acetic acid 10:90
1.0 mL/min
254 nm
1. Saccharin
2. Caffeine
3. Benzoic acid
4. Aspartame
5. Sorbic Acid
Column:
Part Number:
Sample:
In elution order:
Waters Nova-Pak C8,

3.9 x 50 mm
WAT052775
Anticonvulsants
1-Succinic acid imide
2-Primidone
3-Phenobarbital
4-Mephenytoin
5-Mephobarbital
6 Min.
3 Min.
Isocratic Separation of Method 8330 Analytes (Explosives)

Column:
Part Number:
Mobile Phase:
Flow Rate:
Injection volume:
Temperature:
Detection:
Sample:
Waters Nova-Pak C8,

3.9 x 150 mm
WAT035876
82:18 water/isopropanol
1.0 mL/min
100 mL
24 C
UV @ 254 nm
1 ppb explosives concentrated
40-fold on Porapak Rdx, in 60:40
water/acetonitrile
Explosives
Blank
Min.
www.waters.com
155
Nova-Pak Analytical Columns
Nova-Pak Cartridge Columns
Analytical columns with 4 m Nova-Pak packing material gives you an

unparalleled range of separation possibilities.
T he cartridge column requires reusable finger-tightened endfittings which

are purc hased separately. W hen you c hange columns, just replace the
cartridge and use the endfittings again. T he cartridge column assembly
includes one disposable cartridge column and reusable endfittings.
Nova-Pak Analytical Columns

Description
Nova-Pak C18
Particle Size
Dimension
Part No.
4 m
2.1 x 150 mm
3.9 x 75 mm
3.9 x 150 mm
3.9 x 300 mm
4.6 x 150 mm
2.1 x 150 mm
3.9 x 75 mm
3.9 x 150 mm
2.1 x 150 mm
3.9 x 75 mm
3.9 x 150 mm
3.9 x 75 mm
3.9 x 150 mm
3.9 x 300 mm
2.1 x 150 mm
3.9 x 75 mm
3.9 x 150 mm
WAT023655
WAT011670
WAT086344
WAT011695
WAT044375
WAT052735
WAT035877
WAT035876
WAT052740
WAT011675
WAT010656
WAT010270
WAT044245
WAT056920
WAT052745
WAT011680
WAT010025
Nova-Pak C8
4 m
Nova-Pak Phenyl
4 m
Nova-Pak CN HP
4 m
Nova-Pak Silica
4 m
Nova-Pak Cartridge Columns

(Requires endfittings, Part No. WAT037525)
Description
Particle Size
Qty.
Dimension
Part No.
Nova-Pak C18
4 m
Nova-Pak C8
4 m
Nova-Pak Phenyl
4 m
Nova-Pak CN HP
4 m
Nova-Pak Silica
4 m
1/box
3/box
1/box
1/box
3/box
1/box
1/box
1/box
1/box
1/box
3/box
1/box
1/box
1/box
1/box
1/box
3/box
1/box
1/box
1/box
3/box
1/box
1/box
1/box
3/box
3.9 x 50 mm
3.9 x 50 mm
3.9 x 100 mm
3.9 x 150 mm
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
3.9 x 50 mm
3.9 x 100 mm
3.9 x 150 mm
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
3.9 x 50 mm
3.9 x 100 mm
3.9 x 150 mm
3.9 x 150 mm
3.9 x 50 mm
3.9 x 100 mm
3.9 x 150 mm
3.9 x 150 mm
4.6 x 150 mm
4.6 x 250 mm
3.9 x 150 mm
3.9 x 150 mm
WAT052780
WAT052834
WAT052810
WAT036975
WAT037520
WAT052845
WAT052840
WAT052775
WAT052805
WAT036985
WAT054870
WAT052855
WAT052850
WAT052790
WAT052800
WAT036970
WAT054890
WAT052785
WAT052795
WAT044243
WAT044445
WAT044455
WAT044460
WAT036980
WAT054880
Endfittings (includes 1 pair of reusable

endfittings, 2 c-clips and coupling)
WAT037525
Nova-Pak Analytical Method Validation Kit

T he Method Validation Kit includes three Nova-Pak C 18, 4 m, 4.6 x
150 mm steel columns. Two of these columns are packed with material
from one particular batch of material and the other column is packed with
material from a different batch of the bulk material. All three columns are
identified with the batc h number. T he columns are ideal for testing the
ruggedness of new applications for regulated methods. Nova-Pak batchto-batch reproducibility is guaranteed.
156
Nova-Pak Analytical Method Validation Kit

Description
Method Validation Kit
(includes 3 Nova-Pak
C18 columns)
Particle Size
Dimension
Part No.
4 m
3.9 x 150 mm
WAT052770
www.waters.com
Analytical Kits
Sentry Guard Holders

(50 mm and 75 mm long cartridge columns must use the Universal Guard Holder)
Description
Waters Reversed-Phase Scouting Kit
(includes 1 each Nova-Pak C18, C8, and
Phenyl cartridge column)
Waters Method Development Scouting Kit
(includes 1 each Nova-Pak C8, Phenyl, and
CN HP cartridge column)
Waters Method Validation Kit
(includes 3 Nova-Pak C18 columns representing
2 different bonded-silica bulk packing batches)
Dimension
Part No.
3.9 x 50 mm
WAT044360
3.9 x 50 mm
WAT044255
3.9 x 150 mm
WAT052770
Description
Part No.
Integrated Guard Holder

(for Waters 3.9 mm and 4.6 mm cartridge columns)
Universal Guard Holder (for any 3.9 mm and 4.6 mm HPLC column)
WAT046905
WAT046910
Nova-Pak Guard-Pak Inserts 10/pk

(Requires Guard-Pak Holders)
Nova-Pak Sentry Guard Columns 2/pk
Description
(Requires Sentry Guard Holder, Part No. WAT046910)

Description
Nova-Pak C18
Nova-Pak C8
Nova-Pak Phenyl
Nova-Pak Silica
Nova-Pak CN HP
Particle Size
Dimension
Part No.
4 m
4 m
4 m
4 m
4 m
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
WAT044380
WAT046830
WAT046835
WAT046845
WAT046840
Particle Size
Pore Size
Part No.
4 m
4 m
4 m
4 m
4 m
60
60
60
60
60
WAT015220
WAT035880
WAT020795
WAT020800
WAT020790
Nova-Pak C18
Nova-Pak C8
Nova-Pak Phenyl
Nova-Pak CN HP
Nova-Pak Silica
Guard-Pak Holder
Guard-Pak Holder
WAT088141
Prep Nova-Pak HR Packings

Prep Nova-Pak HR 6 m packing is available in both economical column
segments and preparative columns. T he dimensions of the columns have
been chosen specifically to make scale-up from analytical to prep scales
simple and fast. T he high efficiency packing gives you faster separations, using less solvent, with the added advantage of more concentrated
fractions, all of which reduce the cost of your preparative chromatography.
www.waters.com
Prep Nova-Pak HR Analytical and Preparative Columns

Semipreparative and preparative columns for predictable scale-up
from lab scale to process chromatography.
Description
Particle Size
Pore Size
Dimension
Part No.
6 m
60
3.9 x 300 mm
7.8 x 300 mm
19 x 300 mm
WAT038500
WAT025820
WAT025822
Prep Nova-Pak
HR Silica
6 m
60
3.9 x 300 mm
WAT038501
7.8 x 300 mm
19 x 300 mm
WAT025821
WAT025823
157
Bondapak/Bondapak Packings
If your method calls for Bondapak C 18 packings, there is only one
Bondapak C18 packing material. Almost anything can be made once, but
making a packing material reproducible year-to-year for over 30 years
demonstrates true manufacturing and QC capability. Many companies
claim to have columns with Bondapak-like selectivity but not one has
ever passed Waters stringent QC batch test. Waters has set the standard
for reproducible, high-performance c hromatographic columns. Waters
Bondapak columns are still the most widely referenced and requested
columns in the world.
Bondapak/Bondapak Preparative Columns

Particle Size
Pore Size
Dimension
Part No.
Bondapak C18
Description
10 m
125
Bondapak C18
15-20 m
125
10 m
10 m
10 m
125
125
125
7.8 x 300 mm
19 x 150 mm
19 x 300 mm
7.8 x 300 mm
19 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
WAT084176
WAT088500
WAT025828
WAT025832
WAT025834
WAT084177
WAT084178
WAT084179
Bondapak CN
Bondapak NH2
Bondapak Phenyl
Packing
Bondapak
Bondapak
Bondapak
Bondapak
Bondapak
Chemistry
Particle
Size
Particle
Shape
Pore
Size
Carbon
Load
Endcapped
C18
Phenyl
CN
NH2
C18
10 m
10 m
10 m
10 m
15-20 m
Irregular
Irregular
Irregular
Irregular
Irregular
125
125
125
125
125
10%
8%
6%
3.5%
10%
Yes
Yes
Yes
No
Yes
Bondapak C18
Bondapak C18
Bondapak CN
Bondapak NH2
Bondapak Phenyl
Particle Size
10 m
15-20 m
10 m
10 m
10 m
Pore Size
Dimension
Part No.
125
2.1 x 300 mm
3.9 x 150 mm
3.9 x 300 mm
4.6 x 150 mm
4.6 x 300 mm
3.9 x 300 mm
3.9 x 150 mm
3.9 x 300 mm
3.9 x 150 mm
3.9 x 300 mm
WAT086609
WAT086684
WAT027324
WAT044370
186000925
WAT025875
WAT086688
WAT084040
WAT086680
WAT027198
125
125
125
125
(Requires Sentry Holders)

Description
Bondapak C18
Bondapak CN
Bondapak NH2
Bondapak Phenyl
Pore Size
Dimension
Bondapak C18
10 m
125
4.6 x 250 mm
Endfittings
(includes 1 pair of reusable endfittings, 2 c-clips and coupling)
158
10 m
10 m
10 m
10 m
WAT044480
WAT046855
WAT046865
WAT046850
Description
Part No.

Universal Guard Holder (for any 3.9 mm and 4.6 mm HPLC column)
WAT046905
WAT046910
Bondapak Guard-Pak Inserts 10/pk

Part No.
Bondapak C18
Bondapak NH2
Bondapak Phenyl
Bondapak CN
WAT052860
Guard-Pak Holder
WAT037525
Guard-Pak Holder
(Requires endfittings)
Particle Size
Part No.
(50 mm and 75 mm long cartridge columns must

use the Universal Guard Holder)
Description
Bondapak Cartridge Column
Description
Particle Size
Bondapak/Bondapak Analytical Columns

Description
Bondapak Sentry Guard Columns (3.9 x 20 mm) 2/pk
Particle Size
10 m
10 m
10 m
10 m
Pore Size
125
125
125
125
Part No.
WAT088070
WAT026760
WAT026745
WAT026750
WAT088141
www.waters.com
Delta-Pak Columns
Delta-Pak columns, ideal for the separation of peptides, proteins and
natural products, are based on a highly stable, bonded, endcapped 5 m
or 15 m spherical silica. Delta-Pak columns are available in two different
pore size materials (100 and 300) with a C18 or C4 bonded phase.
Delta-Pak columns are available in different cartridge and column configurations, providing consistent and predictable scale-up capabilities for
milligram-to-gram scale purifications. Also, by having the same chemistry
available on a high-efficiency 5 m particle, you can easily check the purity
of your fractions.
Packing
Delta-Pak
Delta-Pak Analytical Columns

Description
Particle Size
Pore Size
Dimension
Part No.
Delta-Pak C18
5 m
Delta-Pak C4
5 m
300
100
300
100
300
2.1 x 150 mm
3.9 x 150 mm
3.9 x 150 mm
3.9 x 150 mm
3.9 x 150 mm
WAT023650
WAT011795
WAT011793
WAT011796
WAT011794
Particle Size
Pore Size
Dimension
Part No.
Delta-Pak C18
15 m
Delta-Pak C4
15 m
100
300
100
300
100
300
100
300
100
100
300
100
300
100
300
100
300
3.9 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
19 x 300 mm
19 x 300 mm
30 x 300 mm
30 x 300 mm
50 x 300 mm
3.9 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
19 x 300 mm
19 x 300 mm
30 x 300 mm
30 x 300 mm
WAT011797
WAT011802
WAT011798
WAT011803
WAT011799
WAT011804
WAT011800
WAT011805
WAT011801
WAT011807
WAT011812
WAT011808
WAT011813
WAT011809
WAT011814
WAT011810
WAT011815
Delta-Pak Preparative Columns

Description
Chemistry
Particle
Size
Particle
Shape
Pore
Size
Carbon
Load
Endcapped
C18
C18
5 m
5 m
Spherical
Spherical
100
300
17%
7%
Yes
Yes
C4
C4
5 m
5 m
Spherical
Spherical
100
300
7%
3%
Yes
Yes
C18
C18
15 m
15 m
Spherical
Spherical
100
300
17%
7%
Yes
Yes
C4
C4
15 m
15 m
Spherical
Spherical
100
300
7%
3%
Yes
Yes
Purification of a Synthetic Peptide Using Delta-Pak C18 Packing

Column:
Part Number:
Sample:
Injection:
Mobile Phase:
Gradient:
Conditions:
Flow Rate:
Detection:
www.waters.com
Delta-Pak C18, 5 m, 300

3.9 x 150 mm
WAT011793
Synthetic peptide-neurotensin (5 mg/mL)
10 L (50 g)
Solvent A: H2O with 0.1% TFA
Solvent B: CH3CN with 0.1% TFA
0-2 min, 5% B
2-27 min, 5-9% B
30-31 min, 90-5% B
1 mL/min
230 nm
Delta-Pak High Pressure Inert (HPI) Analytical Columns (PEEK)

Column (non metallic) Particle Size
Pore Size
Dimension
Delta-Pak HPI C18

5 m
300
3.9 x 150 mm
PEEK tubing and fitting kit
(to minimize damage by stainless steel fittings)
1/16 o.d. x 0.005 i.d. x 5 feet (1.6 mm o.d. x
0.127 mm i.d. x 1.5 mm). Kit contains 5 each of short and
long compression screws, 12 poly knobs and 12 PEEK ferrules.
Part No.
WAT035571
WAT022999
159
Delta-Pak Sentry Guard Columns (3.9 x 20 mm) 2/pk
Delta-Pak Guard-Pak Inserts
(Requires Sentry Guard Holders)
Description
Particle Size
Pore Size
Part No.
5 m
5 m
5 m
5 m
100
300
100
300
WAT046880
WAT046890
WAT046875
WAT046885
Delta-Pak C18
Delta-Pak C18
Delta-Pak C4
Delta-Pak C4
Description
Particle Size
Pore Size
Quantity
Part No.
5 m
5 m
5 m
100
300
300
10/pk
10/pk
10/pk
WAT036870
WAT036875
WAT036865
Delta-Pak C18
Delta-Pak C18
Delta-Pak C4
Guard-Pak Holder
WAT088141

Description
Part No.

Universal Guard Holder
(for any 3.9 mm and 4.6 mm HPLC column)
WAT046905
WAT046910
Porasil/Porasil Silica Packings

Waters Porasil 10 micron packing material provides high-efficiency and
low backpressure. Porasil silica packing material is available in 15-20 and
37-55 micron particle sizes.
Porasil Sentry Guard Columns (3.9 x 20 mm) 2/pk

Description
Particle Size
Part No.
10 m
WAT046860
Porasil
Packing
Chemistry
Particle
Size
Particle
Shape
Pore
Size
Carbon
Load
Endcapped
Porasil
Porasil
Silica
Silica
10 m
15-20 m
Irregular
Irregular
125
125
N/A
N/A
N/A
N/A

Description
Porasil/Porasil Analytical and Preparative Columns

Description
Pore Size
Dimension
Part No.
10 m
125
Porasil
15-20 m
125
3.9 x 150 mm
3.9 x 300 mm
7.8 x 300 mm
19 x 150 mm
19 x 300 mm
3.9 x 300 mm
19 x 300 mm
WAT086692
WAT027477
WAT084175
WAT091648
WAT025829
WAT025874
WAT025835
160
Porasil
Particle Size

Part No.
WAT046905
WAT046910
www.waters.com
Resolve Columns
T he non-endcapped Resolve packings are significantly different from
Nova-Pak and Bondapak packing materials. T he change in chromatographic
be havior is most commonly noticed wit h polar compounds w hic h are
typically more retained. Basic compounds can be chromatographed using
mobile phase modifiers such as ion-pairing reagents which reduce excessive
tailing. Resolve C 18 and Silica columns are available for applications
requiring high resolution.
Resolve Sentry Guard Columns 2/pk

Description
Particle Size
Pore Size
Part No.
Resolve C18
Resolve C8
5 m
5 m
90
90
WAT046915
WAT046920
Packing
Chemistry
Particle
Size
Particle
Shape
Pore
Size
Carbon
Load
Endcapped
Resolve
Silica
C18
C18
C8
CN
Silica
5 m
5 m
10 m
10 m
10 m
10 m
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
90
90
90
90
90
90
N/A
10%
10%
5%
3%
N/A
N/A
No
No
No
No
N/A
Description
Part No.

WAT046905
WAT046910
Resolve Guard-Pak Inserts 10/pk

Resolve Columns
Description
Description
Particle Size
Pore Size
Dimension
Part No.
Resolve C18
5 m
90
Resolve Silica
5 m
90
3.9 x 300 mm
3.9 x 150 mm
3.9 x 150 mm
WAT011740
WAT085711
WAT086016
Resolve C18
Resolve Silica
Resolve C8
Resolve CN
Particle Size
Pore Size
Part No.
10 m
10 m
10 m
10 m
90
90
90
90
WAT085824
WAT085825
WAT026755
WAT085826
Guard-Pak Holder
WAT088141


www.waters.com
Description
Part No.

715001531
715002531
161
Shodex RSpak Polymer Reversed-Phase Columns

Shodex RSpak columns are packed with porous polymeric particles that
are stable at a pH range of 2-12 and have a different selectivity from
silica-based columns. T he wide pH range makes the chromatography of
basic compounds much easier because it is possible to chromatograph
at high pH without ion-pairing salts. Waters offer six different types of
polymeric packings. One of these, the DS-613, is based on a styrene
divinylbenzene copolymer whic h is similar to conventional polymerbased columns and works well with more hydrophobic samples requiring
high concentrations of organic modifiers.
T he DE-613 polymethacrylate and the family of functionalized packings
(D18-613 and D4-613) are naturally more hydrophilic than the DS-613
packing and function well in mobile phases containing high concentrations
of water. T he least hydrophobic of these packings is the DE-613 followed
by the D4-613 and the longer carbon chain length derivative columns.
T he DC-613 is a cation exchanger and has a unique selectivity (mixed
chromatographic mode ion exchange and reversed-phase partition) for
weakly cationic species.
RSpak D Series
Column Type
DS-613
DE-613
DC-613
Base Polymer
Functional Group
Polystyrene
Polymethacrylate
Polystyrene
None
None
Sulfonated
Proteins and Peptides

Column:
Part Number:
Mobile Phase:
Flow Rate:
Detection:
Sample:
Comparison of Basic Compounds on Five Different

RSpak Columns and C18 Based Column
RSpak D18-613, 6 x 150 mm

WAT036576
A: 0.05% TFA: CH3CN (80:20)
B: 0.05% TFA: CH3CN (30:70)
Linear gradient A to B in 30 min
1 mL/min
220 nm
1. Bradykinin
2. Neurotensin
3. Substance P
4. Insulin
5. Insulin B chain
6. Lysozyme
7. Myoglobin
30 Min.
Sample:
Solvent:
Local anesthetics
44% acetonitrile, 56% water,
1% ammonium hydroxide (pH 11)
3 mL/min
220 nm
Flow Rate:
Detection:
2
3
Choosing the Appropriate RSpak Column for your Separation

A: DE - 613
1
2
1
2
B: DS - 613
E: C18
18 min.
Above is a comparison of the separation of three local anesthetics using RSpak columns DE-613, DS-613,
D18-613 and D4-613. Mobile phase contains ammonium hydroxide at pH 11. The compounds separated are
lidocaine, procaine and tetracaine. The pK values of the anesthetics are greater than 9.0, a mobile phase with a
high pH is therefore needed to deprotonate these compounds. Once deprotonated, they are easily separated by a
reversed-phase mechanism. Eluents are simple and easier to reproduce. Compare these to Figure E, a separation
performed on a silica-based C18 column using an ion pairing reagent.
162
If you had no prior experience with Polymeric Reversed-Phase (PRP)

packing materials, the efficient 6 m polymethaceylate based RSpak
DE-613 column would be a good starting point for most applications.
T his column exhibits a mid-range hydrophobicity index equivalent to a
C8 bonded silica phase.
Should increased retention be required for the analyte of interest, the
more hydrophobic SDVB based DS-613 column can be used. Alternatively,
decreased retention is observed with the functionalized methacrylate based
packings such as D18-613 and D4-613 and should be used if analytes are
very hydrophobic.
RSpak D Series Columns

Column
DS-613
DE-613
DC-613
DS-G pre-column
DE-G pre-column
DC-G pre-column
Dimension
Part No.
6 x 150 mm
6 x 150 mm
6 x 150 mm
4.6 x 10 mm
4.6 x 10 mm
4.6 x 10 mm
WAT034220
WAT034221
WAT034223
WAT034224
WAT034225
WAT034227
www.waters.com
Guard Products
Guide to Guard Column Selection and Use
Guard columns are used primarily to protect expensive analytical and
preparative columns by removing particulate and strongly retained
sample components whic h ultimately decrease the lifetime of the
analytical column. Although economy alone is a persuasive argument
for the use of guard columns, the need for a long, stable life in the
analytical column in order to obtain reliable and reproducible results
is per haps even more important.
Guard columns are installed between the injector and the analytical
column of a liquid chromatographic system. Guard columns may also be
used to perform automated, on-line sample cleanup. In situations where
a guard column is not needed, or where no suitable guard column is
available, an in-line filter should be used to protect the analytical column
from microparticulate contamination. T he in-line filter is placed between
the injector and the column.
Guard Column Design

Guard columns are designed with relatively small total volumes and
minimal dead volumes so that they minimize band broadening. T he ratio
of guard column to analytical column volume should be such that adding
a guard column decreases system efficiency by no more than 5-10%.
Generally, a ratio of 1:10 to 1:25 is satisfactory.
T he high-performance Sentry guard column was developed for customers

needing the increased capacity required for biological samples and/
or temperature control. W hen placed in series with an analytical column,
Waters guard column increases overall efficiency. T his efficiency increasing
phenomenon was observed when the Sentry guard column was used with
both short (100 mm) and standard length analytical columns.
T he Sentry guard column gives you double the capacity of traditional guard
columns so that you need to change the column less frequently. In addition
to extending the productive lifetime of your column, the Sentry guard
column enhances the reproducibility giving you consistent, reliable,
reproducible results time after time.
Sentry guard columns contain the same high-performance packing
materials that are used in all Waters analytical columns. T hese convenient, easy-to-use, disposable guard columns are an economical means
of protecting your more expensive analytical column. You can c hange
the guard column in seconds.
Two holder designs are offered, one to be used as an integrated part of
the Waters cartridge column with reusable endfittings, the other for use
with any HPLC column. Both allow Sentry guard columns to be replaced
without tools.
Although larger guard columns would have the capacity to trap impurities from more sample injections, excessive band broadening would result.
Waters offers two configurations for guard columnsthe Sentry guard
holders and Guard-Pak holder. T he Sentry guard holder is available in two
designs: one to be used as an integrated part of the cartridge column with
reusable endfittings; the other for use with any HPLC column. Both are
installed without tools. T he Guard-Pak holder is a convenient and inexpensive device since you can use prepacked Guard-Pak inserts matched to the
chemistry of your analytical column.
Positioning of a Guard Column in an LC System
Solvent
Delivery
System
Detector
Injector
Guard Column
or Inline Filter
HPLC Column
Flow

Description
Waters Sentry Guard Holders and

Sentry Guard Columns
Guard columns are widely used as a cost effective means of prolonging
HPLC column life. Guard columns protect the column from particulate
matter and chemical contaminants.
www.waters.com
Part No.
Integrated guard holder (for Waters cartridge columns)

Universal guard holder (for any HPLC column)
Sentry 2.1 mm guard holder kit
(includes guard holder and rigid connector)
O-Ring kit (2/pk) for Sentry 2.1 mm guard holder
Rigid connector for Sentry 2.1 mm guard holder
2.1 x 20 mm guard holder
WAT046905
WAT046910
WAT097958
WAT097954
WAT022681
186000262
163
Sentry Guard Columns
Symmetry Sentry Guard Columns, 2/pk

Description
XBridge Sentry Guard Columns, 2/pk

Description
Dimension
2.5 m
3.5 m
5 m
XBridge C18
2.1 x 10 mm
3.0 x 20 mm
4.6 x 20 mm
2.1 x 10 mm
3.0 x 20 mm
4.6 x 20 mm
2.1 x 10 mm
3.0 x 20 mm
4.6 x 20 mm
2.1 x 10 mm
3.0 x 20 mm
4.6 x 20 mm
186003056
186003057
186003058
186003074
186003075
186003076
186003065
186003066
186003067
186003359
186003360
186003361
186003059
186003060
186003061
186003077
186003078
186003079
186003068
186003069
186003070
186003362
186003363
186003364
186003062
186003063
186003064
186003080
186003081
186003082
186003071
186003072
186003073
186003366
186003367
186003368
XBridge C8
XBridge Shield RP18
XBridge Phenyl
Description
Dimension
2.5 m
3.5 m
5 m
SunFire C18
2.1 x 10 mm
3.0 x 20 mm
4.6 x 20 mm
2.1 x 10 mm
3.0 x 20 mm
4.6 x 20 mm
186003395
186003405
186003413
186003396
186003406
186003414
186002530
186002681
186002682
186002708
186002718
186002727
186002536
186002683
186002684
186002713
186002722
186002733
Atlantis Sentry Guard Columns, 2/pk

Description
Dimension
3 m
5 m
Atlantis T3
2.1 x 10 mm
3.9 x 20 mm
4.6 x 20 mm
2.1 x 10 mm
2.1 x 20 mm
3.9 x 20 mm
4.6 x 20 mm
2.1 x 10 mm
3.9 x 20 mm
4.6 x 20 mm
186003756
186003757
186003758
186001377
186001381
186001313
186001321
186002005
186002021
186002023
186003759
186003760
186003761
186001379
186001383
186001315
186001323
186002006
186002022
186002024
Atlantis dC18
Atlantis HILIC
XTerra Sentry Guard Columns, 2/pk

Description
XTerra MS C18
XTerra MS C18
XTerra MS C18
XTerra MS C18
XTerra MS C8
XTerra MS C8
XTerra MS C8
XTerra MS C8
XTerra RP18
XTerra RP18
XTerra RP18
XTerra RP18
XTerra RP8
XTerra RP8
XTerra RP8
XTerra RP8
164
Dimension
3.5 m
5 m
2.1 x 10 mm
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
2.1 x 10 mm
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
2.1 x 10 mm
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
2.1 x 10 mm
2.1 x 20 mm
3.0 x 20 mm
3.9 x 20 mm
186000632
186000636
186000640
186000644
186000633
186000637
186000641
186000645
186000634
186000638
186000642
186000646
186000635
186000639
186000643
186000647
186000648
186000652
186000656
186000660
186000649
186000653
186000657
186000661
186000650
186000654
186000658
186000662
186000651
186000655
186000659
186000663
Particle Size
Part No.
2.1 x 10 mm
3.9 x 20 mm
2.1 x 10 mm
3.9 x 20 mm
2.1 x 10 mm
3.9 x 20 mm
2.1 x 10 mm
3.9 x 20 mm
2.1 x 10 mm
3.9 x 20 mm
2.1 x 10 mm
3.9 x 20 mm
3.5 m
5 m
3.5 m
5 m
3.5 m
5 m
3.5 m
5 m
3.5 m
5 m
3.5 m
5 m
WAT106127
WAT054225
WAT106128
WAT054250
186000169
186000107
WAT106129
WAT200675
186000275
186000284
186000198
WAT106166
Dimension
Particle Size
Part No.
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
4 m
4 m
4 m
4 m
4 m
WAT044380
WAT046830
WAT046835
WAT046840
WAT046845
Dimension
Particle Size
Part No.
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
10 m
10 m
10 m
10 m
WAT044480
WAT046855
WAT046865
WAT046850
Dimension
Particle Size
Part No.
3.9 x 20 mm
10 m
WAT046860
Nova-Pak Sentry Guard Columns, 2/pk

Description
SunFire Sentry Guard Columns, 2/pk
SunFire C8
Symmetry C18
Symmetry C18
Symmetry C8
Symmetry C8
SymmetryShield RP18
SymmetryShield RP18
SymmetryShield RP8
SymmetryShield RP8
Symmetry300 C4, 300
Symmetry300 C4, 300
Symmetry300 C18, 300
Symmetry300 C18, 300
Dimension
Nova-Pak C18
Nova-Pak C8
Nova-Pak Phenyl
Nova-Pak CN HP
Nova-Pak Silica
Bondapak Sentry Guard Columns, 2/pk

Description
Bondapak C18
Bondapak CN
Bondapak NH2
Bondapak Phenyl
Porasil Sentry Guard Columns, 2/pk

Description
Porasil
Delta-Pak Sentry Guard Columns, 2/pk

Description
Delta-Pak C18 100
Delta-Pak C18 300
Delta-Pak C4 100
Delta-Pak C4 300
Dimension
Particle Size
Part No.
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
3.9 x 20 mm
5 m
5 m
5 m
5 m
WAT046880
WAT046890
WAT046875
WAT046885
High Performance Carbohydrate Sentry Guard Columns, 2/pk

Description
High-Performance Carbohydrate
Sentry Guard Column
Dimension
Particle Size
Part No.
3.9 x 20 mm
4 m
WAT046895
Resolve Sentry Guard Columns, 2/pk

Description
Dimension
Particle Size
Part No.
Resolve C18
Resolve C8
3.9 x 20 mm
3.9 x 20 mm
5 m
5 m
WAT046915
WAT046920
www.waters.com
Guard-Pak: Inexpensive Column Protection
Guard-Pak Holder
Guard-Pak Inserts
Waters Guard-Pak Holder is a compact, stand-alone housing for our unique

disposable Guard-Pak inserts. Installed on-line with your HPLC system
immediately before the analytical column, the Guard-Pak holder and
inserts protect analytical LC columns against the gradual accumulation of
particulates and chemical contaminants originating from the sample.
Guard-Pak inserts protect your analytical columns from damage due to

irreversibly adsorbed chemical contaminants or particulates. T he result is
enhanced chromatographic reproducibility and improved column lifetime.
T he inexpensive, disposable plastic inserts are prepacked with analytical
grade 4, 5, and 10 m packing materials and sealed with 2 m filters.
Unlike hand-packed guard columns, Guard-Pak inserts have a consistent,
densely packed bed and are designed to eliminate voids thus ensuring
column-to-column reproducibility.
Guard-Pak inserts prolong column life by removing contaminants from

the sample and mobile phase, giving you enhanced reproducibility
and performance. They are packed with the same high performance
packings used in Waters analytical columns.
Description
Part No.
Guard-Pak Holder
Guard-Pak Holder Connector
WAT088141
WAT080046
Description
Nova-Pak C18
Nova-Pak C8
Nova-Pak Phenyl
Nova-Pak Silica
Nova-Pak CN HP
Bondapak C18
Bondapak NH2
Bondapak Phenyl
Bondapak CN
Resolve C18
Resolve Silica
Resolve C8
Resolve CN
Delta-Pak C18
Delta-Pak C18
Delta-Pak C4
Guard-Pak In-Line Filters
Qty.
Particle Size
Pore Size
Part No.
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
5/pk
4 m
4 m
4 m
4 m
4 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
5 m
5 m
5 m
60
60
60
60
60
125
125
125
125
90
90
90
90
100
300
300
WAT015220
WAT035880
WAT020795
WAT020790
WAT020800
WAT088070
WAT026760
WAT026745
WAT026750
WAT085824
WAT085825
WAT026755
WAT085826
WAT036870
WAT036875
WAT036865
WAT032472
Waters Cartridge and Guard Column Guide

Guard Columns
Universal Sentry Guard Holder (Use on any column)
2.1 x 10 mm use Part No. WAT097958
2.1 x 20 mm use Part No. 186000262
Universal Sentry
Guard Holder
Cartridge Columns
Cartridge End-Fittings
2.1 x 50 mm, 2.1 x 100 mm, 2.1 x 150 mm, 2.1 x 250 mm use Part No. 700000117
3.0 x 50 mm, 3.0 x 100 mm, 3.0 x 150 mm, 3.0 x 250 mm use Part No. WAT037525
Integrated Sentry Guard Holder to go with
cartridge end-fittings (see above)
Cartridge with End-Fittings
Cartridge with Integrated Guard Column
Sentry Guard
Column
www.waters.com
165
Paired-Ion Chromatography (PIC Reagents)

An easy solution to the problem of analyzing samples containing ionic
species, Waters PIC reagents allow ionic compounds to be separated by
reversed-phase chromatography while eliminating problems of precise pH
and temperature control, reproducibility and short column life associated
with ion exchange. PIC reagents versatility can save time by allowing simultaneous assay of acids, bases, amphoteric and neutral compounds. Waters
PIC reagents are:
Purified and quality controlled by liquid

c hromatography to guarantee consistent results
Preformulated for ease of use
Buffered to an appropriate pH for most acidic
and basic compounds
Use Low UV PIC to analyze ionic compounds at low UV (200 nm). Low UV
PIC is specially formulated to eliminate background interference in the low
UV range and provides:
Alkaloids
Cephaeline
Emetine
Ergot alkaloids
Analgesics/Antipyretics
APC
Aspirin
Codeine
Indomethacin
Meperidine
Methadone
Morphine
Propoxyphene
Antibiotics
Cephalosporins
Penicillins
Antihistamines
Chlorpheniramine
Diphenhydramine
Methapyrilene
Phenimdamine
Pheniramine
Pyrilamine
Tripelennamine
PIC Reagent
B
B
B
A
A
B
A
B
B
B
B
A or B
A or B
B
B
B
B
B
B
B
Compound
or Class
Antitussives
B Vitamins
Calcium Leucovorin
FD&C Dyes
Folic Acid
Germicides
Benzalkonium Chloride
Cetylpyridinium
Imides
Methotrexate
Petroleum Sulfonates
Surfactants
Quaternary amines
Sulfonates
Sympathomimetics
(Decongestants)
Amphetamine
Ephedrine
Epinephrine
Isoproterenol
Naphazoline
Nylidrin
Phenylephrine
Phenylpropanolamine
Choice of B Reagent will depend upon the mixture to be separated.

B7 is the recommended initial choice.
166
FD&C Dyes Cough Syrup

Column:
Part Number:
Solvent:
Sample:
Methods development opportunities for ion pairs

whic h do not have a c hromophore in the working
range of regular PIC reagents
Increased sensitivity for compounds ordinarily
used with existing PIC reagents
Potential for selectivity c hanges due to alternative
buffers as compared to regular PIC reagents
Compound
or Class
Use PIC B for rapid separation of bases. Quaternary amines, another class
of compounds that is usually separated by ion-exchange chromatography,
can be more easily separated by Paired-Ion Chromatography employing one
of the PIC Reagent B series.
Use Reagent PIC D-4 as a mobile-phase modifier when chromatographing
basic pharmaceutical compounds to improve peak shape and reduce
retention times.
T he accompanying table lists some typical compounds whic h can be

c hromatographed using Paired-Ion Chromatography. A Bondapak C18
column with a water/methanol solvent system was used in all cases.
Use PIC A for rapid separation of acids. FD&C sulfonic acid dyes are classic
examples of compounds that are conventionally separated by ion-exchange
chromatography. Using PIC Reagent A and reversed-phase chromatography,
the sulfonic acid dyes and their impurities can be separated easier, faster,
and more reliably.
Flow Rate:
Detection:
Bondapak C18, 3.9 x 300 mm

WAT027324
A) H2O with PIC Reagent A
B) CH3OH with PIC Reagent A
40% B - 55% B
80 L Cough Syrup (diluted 1/1
with water)
2 mL/min
254 nm
PIC techniques can be used to determine trace

impurities in bulk dyes or to quantitate dyes in a
complex mixture, such as a cough syrup
PIC Reagent
B
B5/B7
A
A
A
B
B
A
A
A
B
A
B
B
B
B
B
B
B
B
Workable
UV Range in
Mobile Phase (nm)
PIC A (tetrabutylammonium hydrogen sulfate)
Low UV PIC A
PIC A (tetrabutylammonium phosphate
PIC B5 (pentane sulfonic acid)
Low UV PIC B5
PIC B5
PIC B6 (hexane sulfonic acid)
Low UV PIC B6
PIC B6
PIC B7 (heptane sulfonic acid)
Low UV PIC B7
PIC B7
PIC B8 (octane sulfonic acid)
Low UV PIC B8
PIC B8
PIC Sample Kit (One vial each: PIC A,
B5, B6, B7 and B8)
PIC Reagent D4 (dibutylammonium phosphate)
Quantity
(Vials/
Pkg)
Part No.
200+
240+
5
5
WAT084189
WAT085101
200+
240+
5
5
WAT084198
WAT085110
200+
240+
5
5
WAT084199
WAT085140
200+
240+
5
5
WAT084282
WAT085103
200+
240+
5
5
WAT084283
WAT085142
215+
5
5
WAT085144
WAT085466
www.waters.com
Sugar and Carbohydrate Analysis

Carefully controlling the content of commercial foods and beverages is the
fundamental task of any manufacturer. Quality control is of premier importance, not only for compliance with regulatory agencies, but for customer
satisfaction as well.
T he primary ingredients of foods and beverages, which must be analyzed
and quantitated, are sugars and carbohydrates, fatty acids, triglycerides and
cholesterol, pesticides, organic acids, alcohols, caffeine, and other additives.
Waters offers a range of columns for analysis of each of these classes of
compounds.
Sugars constitute a very important class of compounds which are ubiquitous
in nature. T hey are found in food, microorganism, and paper pulp, and are
involved in many biochemical interactions. Sugars present a challenge for
separations because of the small differences in their chemical and physical
properties. In order to exploit these minor differences and effect a separation, several modes of chromatography exist for the analysis of sugars.
Reversed-Phase
Reversed-phase is best suited to the separation of mixtures containing
monosaccharides and oligosaccharides up to a degree of polymerization
(DP) of 8 as applies to monitoring acid or enzyme hydrolysates of large
oligosacc harides. T his mode is also amenable for the separation of
derivatized sugars.
Size Exclusion
Gel-Permeation Chromatography (GPC) is used to determine the molecular
weight distribution of food polymers such as starches and gums. T he Waters
Ultra hydrogel columns are well suited for this application. T he Shodex
KS Series (7 m) and robust S Series (10 m) packings are based on a
sulfonated styrene divinylbenzene polymer. Designed to analyze and
c haracterize water-soluble polymers, they are particularly well suited
for mono-, di- and polysacc harides. T he KS-800 Series is also useful
for separation of intermediate and small DP sugars.
Selecting the Separation Mode

T here are many different modes of chromatography used to characterize
carbohydrates and many different columns from which to choose. Before
c hoosing a column, an understanding of the types of columns that are
available and the mode of chromatography that can be used to exploit the
differences between sugars would be beneficial.
Ion Exchange
Selecting the Column

T he table on page 168 lists the various columns along with the
associated separation mode, typical mobile phases, and expected
order of carbohydrate elution.
For Shodex columns, the name of a particular column contains all the
pertinent information necessary to define the column. T hese factors are
listed in the second table.
T his mode provides selectivity to separate mono- and some disaccharides,

as well as sugar alcohols and low molecular weight alcohols, by hydroxyl
coordination to the metal cation. Ion exc hange uses sulfonated styrene
divinylbenzene resins in lead form (Shodex SP-0810) or calcium form
(Shodex SC-1011, or our Sugar-Pak I). T he mobile phase is water at
80-90 C.
Partition
Partition chromatography is best suited for low molecular weight sugars
such as mono-, di-, and trisaccharides. T his mode uses a covalently bonded
amino packing in a column such as our Carbohydrate Analysis column, or
a dynamically-coated silica cartridge employing Silica Amine Modification
(SAM) reagents such as SAM I. Typical mobile phases range from 65-85%
acetonitrile in water, and the separations are carried out at temperatures
ranging from ambient to 70 C.
www.waters.com
167
Typical Applications for Sugar and Carbohydrate Columns
Cartridge/Column
Mode
Eluent
Application
Elution Order
Carbohydrate analysis column
Partition
65 to 85% CH3CN:H2O
ambient to 70 C
Mono-, di- and trisaccharides

up to DP 8
Smallest elute first
Sugars and sugar alcohols

SAM I reagent with silica cartridge
Partition
70 to 80% CH3CN:H2O
0.1% SAM I ambient
Mono-, di- and trisaccharides
Sugar-Pak I, SC-1011, SP-0810
Ion exchange/
size exclusion
H2O
75-95 C
Mono-, di-, oligosaccharides

and sugar alcohols
Largest elute first
SH-1011, IC-Pak ion exclusion

Fast fruit juice
Ion exchange/
size exclusion
0.01N H3P04
50-60 C
Sugar acids, sugar alcohols

organic acids
Largest and most acidic elute first
Dextro-Pak
Reversed-phase
H2O
ambient
Hydrolysed syrups
derivatized sugars
KS-800 series
Size exclusion
Mono- through oligosaccharides

such as syrups
Largest elute first
A Guide to Shodex Sugar Columns

S
Type of
column
S = sugar
18
Cation
H = H+
C = Ca2+
P = Pb2+
Z = Zn2+
% cross
linkage
Pore Size
1 = 20
2 = 50
3 = 100
4 = 500
5 = 1000
0 - Gel type
1 - Semimacropore gel
2 - Permanent pore gel
Example:
S
Sugar column
168
Ca2+
10
10% cross linkage
20 pore size
semimacropore gel
www.waters.com
Retention Times for Sugar and Carbohydrates
Column Type
Carbohydrate
Analysis
Sugar-Pak
Dextro-Pak
Silica-Pak
KS-801
SC-1011
SP-0810
SH-1011
Column Temp:
Ambient
90 C
Ambient
75 C
80 C
80 C
60 C
Mobile Phase:
80:20
Acetonitrile:
Water
2 mL/min
Water
Water
Water
Water
Water
Water
0.5 mL/min
1 mL/min
Ambient
SAM I Reagent
75:25
Acetonitrile:
Water
4 mL/min
1 mL/min
1 mL/min
1 mL/min
1 mL/min
8.4
9.2
7.7
9.8
9.0
11.1
10.7
10.8
9.3
13.5
11.1
16.5
7.9
8.6
7.4
7.7
7.5
8.0
5.4
7.9
5.5
9.3
10.6
12.7
20.8
9.0
8.9
8.2
9.6
7.6
11.5
11.2
10.4
9.0
8.7
6.4
6.6
7.1
9.3
13.6
15.3
8.1
8.6
9.9
11.4
13.2
6.6
6.8
7.2
8.3
7.5
7.9
11.3
16.5
11.4
8.7
7.3
13.3
7.0
13.5
7.0
9.8
11.5
9.1
6.1
8.3
13.5
13.2
8.2
5.7
6.4
6.4
6.6
13.0
8.1
7.5
10.3
19.9
22.3
10.2
7.1
8.0
8.1
8.5
20.5
9.7
5.1
7.3
7.9
7.6
6.7
4.9
Flow Rate:
Compound
2-Deoxygalactose
2-Deoxyribose
2-Deoxy-glucose
Adonitol (Ribitol)
Arabinose
Arabitol
Butanol-1
Cellobiose
Deuterium Oxide
Digitose
Digitoxose
Dulcitol
Ethylene Glycol
Ethanol
Fructose
Fucose
Galactose
Glucoheptose
Glucose
Glucose-6-phosphate
Glycerol
Inositol
Isomaltitol
Isomaltose
Lactose
Lactulose
Lyxose
Maltitol
Maltoheptaose
Maltohexaose
Maltopentaose
Maltose
Maltotetraose
Maltotriose
Maltotriol
Mannitol
Mannose
Melibiose
Melizitose
meso-Erythritol
Methanol
myo-Inositol
Pannose
Pentaerythritol
Propanol-2
Raffinose
Rhamnose
Ribose
Sorbitol
Sorbose
Stachyose
Sucrose
Trehalose
Turanose
Xylitol
Xylose
Xylulose
www.waters.com
Approximate Elution Time in Minutes
3.9
4.8
4.8
8.8
11.0
10.4
3.4
2.9
3.0
1.5
1.8
1.8
N/R
15.3
18.8
6.7
N/R
3.7
4.1
8.3
5.7
11.1
N/R
N/R
5.7
4.2
7.7
12.6
12.8
10.1
10.4
9.4
3.5
6.8
2.9
4.1
2.8
1.0
2.0
1.6
2.5
9.3
10.0
7.7
8.1
7.6
7.3
N/R
N/R
8.5
4.8
12.1
2.8
2.1
3.2
2.4
7.2
1.2
8.5
18.0
16.3
4.1
7.1
7.6
10.7
3.0/3.2*
3.8
3.0
4.8
4.0
5.9
5.9
6.0
15.7
6.8
3.5
4.1
4.7
5.9
N/R
6.0
4.4/4.7*
7.8
5.3
7.0
6.4
21.6
12.2
9.6
7.0
2.9
2.9
3.1
2.2
2.2
5.3
7.2
7.6
5.9
5.1
N/R
12.5
3.9
5.9
5.0
12.6
6.0
9.5
14.9
14.2
9.4
5.4
6.8
N/R
7.0
3.3
3.1
2.9
2.8
6.3
4.4
N/R
N/R
3.6
6.9
5.6
N/R
11.9
5.1
4.3
3.3
14.3
9.3
10.6
2.9
2.9
3.1
7.5
1.4
2.2
17.5
3.4
1.8
1.6
5.2
7.3
9.1
7.5
7.4
4.8
5.8
5.7
8.0
7.7
5.4
5.3
5.5
5.8
6.1
5.9
11.1
8.3
6.4
6.0
10.1
9.1
8.4
7.6
7.1
7.8
6.9
6.6
6.6
5.6
5.8
7.4
6.1
4.6
4.6
4.7
5.6
4.9
5.1
5.4
7.4
6.9
5.6
5.1
8.4
5.6
5.8
8.2
7.0
N/R
*
Blank
Not recommended under these conditions

due to insufficient or excessive retention.
Elution times of anomers
No data exists
169
High-Performance Carbohydrate Analysis

Cartridge Column
Waters High-Performance Carbohydrate cartridge column with reusable
endfittings is based on a 4 m, spherical silica. T his column was developed
to separate 5 monosaccharide and disaccharides with base line resolutions
in less than 12 minutes. T he High-Performance Carbohydrate cartridge
column can be run at 1.4 mL/min to achieve 12-minute separations and
is available in a 4.6 mm i.d. x 250 mm cartridge column. T his column
dimension was found to be t he optimum for speed, resolution and
longevity. T he prepacked, disposable cartridge column requires reusable
endfittings which can be purchased separately. W hen you change columns,
just replace the cartridge and use the endfittings again.
You can increase the lifetime of the column by using a Sentry guard
column. Contaminants are removed from the sample and mobile phase
before they reach the column.
Description
High-Performance Carbohydrate cartridge column
(requires endfittings)
Endfittings (End Connector Kit)
(contains 1 pair endfittings, and c-clips)
Sentry High-Performance Carbohydrate
guard column (2/pk)
Sentry Integrated Guard Holder
(for Waters cartridge columns)
Dimension
Part No.
4.6 x 250 mm
WAT044355
Shodex KS-800 Columns

Column
Exclusion Limit
103
KS-801
KS-802
KS-804
KS-800P pre-column
1x
1 x 104
4 x 105
Dimension
Part No.
8 x 300 mm
8 x 300 mm
8 x 300 mm
6 x 50 mm
WAT034276
WAT034277
WAT034279
WAT034282
Waters Ultra hydrogel Columns

Pore
Size
Exclusion
Limit (PEO)
Dimension
Part No.
120
250
500
1000
2000
5 x 103
8 x 104
4 x 105
1 x 106
7 x 106
7.8 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
WAT011520
WAT011525
WAT011530
WAT011535
WAT011540
Ultrahydrogel Linear Blend

Ultrahydrogel DP
120
Ultrahydrogel Guard Column
Ultrahydrogel Guard Column DP
7 x 106
5 x 103
7.8 x 300 mm
7.8 x 300 mm
WAT011545
WAT011550
WAT011565
WAT011570
Column
Ultrahydrogel 120
Ultrahydrogel 250
Ultrahydrogel 500
Ultrahydrogel 1000
Ultrahydrogel 2000
WAT037525
3.9 x 20 mm
WAT046895
WAT046905
Columns and Accessories

Description
Dimension
Part No.
Sugar-Pak 1 column
Sugar-Pak 1 Guard-Pak inserts, 10/pk
6.5 x 300 mm
WAT085188
WAT015209
Carbohydrate Analysis column
3.9 x 300 mm
WAT084038
SC-1011 column
SP-0810 column
SC-1011P pre-column
SP-0810P pre-column
8 x 300 mm
8 x 300 mm
6 x 50 mm
6 x 50 mm
WAT034238
WAT036954
WAT034244
WAT034245
Silica-Pak HP cartridge, 4 m
Silica-Pak cartridge, 10 m
8 x 100 mm
8 x 100 mm
WAT010932
WAT010935
SAM I Reagent, 5/box
WAT010873
Dextro-Pak cartridge column
170
8 x 100 mm
WAT085650
www.waters.com
Fatty Acid Analysis
Pesticide Analysis
Fatty acid analysis can be carried out by the reversed-phase mode. T he

Free Fatty Acid HP column uses a phenyl-bonded packing and a simple
isocratic elution method to separate free fatty acids based on carbon
c hain length and degree of saturation. T he short column dimension
(3.9 x 150 mm) significantly reduces analysis time and increases
sensitivity. Typical applications include free fatty acids, fatty acid
derivatives, and some fatty amides, such as saponified coconut oil, tall
oils, fatty alcohols, and fatty acid isomers.
W hen used with the Waters Carbamate Analysis System, the Carbamate
Analysis column provides a guaranteed analysis of the carbamate pesticides
that exceeds the sensitivity required by AOAC Method 985.23.
Straight c hain saturated acids elute in order of

increasing carbon number
Carbamate Analysis
Unsaturated acids elute before the analgous

saturated compound
Column:
Part Number:
Mobile Phase:
For a given carbon number and c hain configuration,

the greater the unsaturation, the earlier the elution
Flow Rate:
Sample:
Injection Volume:
Post Column
Addition:
Detector:
Fatty Acid Standards

Column:
Part Number:
Mobile Phase:
Flow Rate:
Detection:
T he baseline resolution and high sensitivity of this separation, coupled with

the optimized system configuration, provide state-of-the-art analysis of
carbamates. T he separation of eleven carbamate pesticides and carbamate
metabolites is accomplished in 20 minutes.
Free Fatty Acid HP

WAT011690
CH3CN: THF: H20 45:20:35
1.5 mL/min
RI
Carbamate Analysis column

3.9 x 150 mm
WAT035577
Water/methanol/acetonitrile
complex gradient
1.5 mL/min
10 ng of each analyte on column
400 L
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
OPA/NaOH @ 0.5 mL/min

Model 470 Fluorescence Detector
Excitation: 339 nm lambda
Emission: 445 nm lambda
Aldicarb sulfoxide
Aldicarb sulfone
Oxamyl
Methomyl
3-Hydroxycarbofuran
Aldicarb
Propoxur
Carbofuran
Carbaryl
1-Naphthol
Methiocarb
5
9
Sample
1.
2.
3.
4.
5.
6.
7.
8.
9.
Capric Acid
Lauric Acid
Myristic Acid
Palmitic Acid
Stearic Acid
Nonadecanoic Acid
Arachidic Acid
Heneicosanoic Acid
Behenic Acid
1
7
2
6
11
10
5 Min.
20 Min.
Free Fatty Acid HP Column

Column
Free Fatty Acid HP
www.waters.com
3 4
Carbamate Analysis Column for Pesticides
Dimension
Particle Size
Part No.
3.9 x 150 mm
4 m
WAT011690
Column
Carbamate Analysis
Dimension
Part No.
3.9 x 150 mm
WAT035577
171
Waters PAH Columns

PAH Analysis using Waters PAH Columns
Column:
Waters PAH Column 5 m,

4.6 x 250 mm @ 27 C
186001265
Waters Alliance System with
2996 Photodiode Array Detector
Water
Acetonitrile
60% B to 100% B using curve 9
in 12 minutes, hold 11 minutes,
back to initial conditions
1.2 mL/min
20 L
EPA-610 mixture
Part Number:
System:
Eluent A:
Eluent B:
Gradient:
Flow Rate:
Injection:
Sample:
Waters PAH Columns Improve

Analysis of PAH Compounds
UV @ 254 nm
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
Naphthalene - 20 ppm
Acenaphthylene - 40 ppm
Acenaphthene - 20 ppm
Fluorene - 4 ppm
Phenanthrene - 2 ppm
Anthracene - 2 ppm
Fluoranthene - 4 ppm
Pyrene - 2 ppm
Benzo(a)anthracene - 2 ppm
Chrysene- 2 ppm
Benzo(b)fluoranthene - 4 ppm
Benzo(k)fluoranthene - 2 ppm
Benzo(a)pyrene - 2 ppm
Dibenzo(a,h)anthracene - 4 ppm
Benzo(g,h,I)perylene - 4 ppm
Indeno(1,2,3-cd)pyrene - 2 ppm
11
10
Polynuclear Aromatic Hydrocarbons (PAHs) are among the most frequently

monitored environmental contaminants. Standard and official methods for
the analysis of PAHs are found in compendia for air, drinking water, wastewater, solid waste, and food analysis.1
1
5
4
Waters PAH columns are optimized for the HPLC analysis of PAHs. T he
c hromatogram (top right) shows 16 PAH compounds, listed as target
pollutants by the U.S. EPA. T he Waters PAH columns ac hieve baseline
resolution and excellent peak symmetry for all 16 target analytes in
less than 25 minutes, while employing a simple water:acetonitrile
binary gradient. T he resolving power of the PAH Columns provides
superior peak identification and quantitation for PAHs.
Florida Administrative Code 17.700 includes 2 additional compounds
(1-methyl naphthalene and 2-methyl naphthalene ) in addition to the 16
compound EPA 610 mix that we currently use to show the proficiency of
Waters instrumentation to analyze PAH compounds (bottom right). T he new
Waters PAH columns resolve these two compounds along with the other 16.
Waters PAH columns come in seven different dimensions (including a
capillary format), and two particle sizes. Eac h column comes with a
complete Certificate of Analysis backed by a world-class ISO 9002
registered documentation trail.
7
12
13
Many of these methods specify HPLC, usually with UV and fluorescence

detection, as the recommended analytical procedure.
8
10
12
14
14
16
18
20
15
22
16
24 Min.
PAH Analysis According to Florida Administrative Code 17.700

Column:
Waters PAH Column 5 m,

4.6 x 250 mm @ 27 C
186001265
Water
Acetonitrile
60% B to 100% B using curve 9
in 12 minutes, hold 11 minutes,
back to initial conditions
1.2 mL/min
20 L
EPA-610 mixture plus two compounds*
Part Number:
Eluent A:
Eluent B:
Gradient:
Flow Rate:
Injection:
Sample:
UV @ 254 nm
Reference:
1
Peaks :
12
AOAC 973.30; Deutsche DIN TVO; UK ISBN 0 11 & 752032 2;

U.S. EPA Methods TO-13, 550 & 550.1, 610, 8310
Peaks :
15
11
4*
9 10
14
Naphthalene - 20 ppm
Acenaphthylene - 40 ppm
1-methyl naphthalene - 25 ppm
2-methyl naphthalene - 25 ppm
Acenaphthene - 20 ppm
Fluorene - 4 ppm
Phenanthrene - 2 ppm
Anthracene - 2 ppm
Fluoranthene - 4 ppm
Pyrene - 2 ppm
Benzo(a)anthracene - 2 ppm
Chrysene - 4 ppm
Benzo(b)fluoranthene - 4 ppm
Benzo(k)fluoranthene - 2 ppm
Benzo(a)pyrene - 2 ppm
Dibenzo(a,h)anthracene - 4 ppm
Benzo(g,h,I)perylene -4 ppm
Indeno(1,2,3-cd)pyrene -2 ppm
13
2
3*
1.
2.
3*.
4*.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
16
11
13
15
17
19
21
17
18
23
25 Min.
Waters PAH Columns

Description
Waters PAH Column
Waters PAH Column
Waters PAH Column
Waters PAH Column
Waters PAH Column
Waters PAH Column
Waters PAH Column
172
Dimension
Particle Size
Part No.
4.6 x 250 mm
4.6 x 150 mm
4.6 x 50 mm
3.0 x 250 mm
2.1 x 250 mm
2.1 x 150 mm
0.32 x 150 mm
5 m
5 m
3 m
5 m
5 m
5 m
5 m
186001265
186001264
186001260
186001263
186001262
186001261
186001259
www.waters.com
Triglycerides and Cholesterol Analysis

Non-aqueous, reversed-phase c hromatography is the best method of
separating triglycerides into classes based on carbon number and
degree of unsaturation. T his method aids in measuring oil degradation
and adulteration. Waters Nova-Pak C18 column is an excellent choice for
this separation due to its high efficiency and carbon surface area. It can
also be used for cholesterol determination from samples, such as meat
following saponification.
T he analysis of high levels of c holesterol in samples like butter and
antioxidants in oil, without saponification, can be effectively performed
by size exclusion using Waters Ultrastyragel columns.
Nova-Pak Column for Triglycerides and Cholesterol Analysis
Column
Nova-Pak C18
Cholesterol From Butter

Column:
Mobile Phase:
Flow Rate:
Sample:
Injection Volume:
Detection:
Ultrastyragel 100 and 500

each column 7.8 x 300 mm
CHCl3
1.0 mL/min
A: Cholesterol standard, 0.125%
B: Butter (1/10 w/v) = 0.24% in
undiluted sample
50 L
RI 410 Refractometer @ 4x
Dimension
Particle Size
Part No.
3.9 x 150 mm
4 m
WAT086344
22 Min.
Ultrastyragel Column for Cholesterol Analysis

Pore
Size
100
500
Effective Molecular
Weight Range
Dimension
Packed in
Toluene
Packed in
THF
50-1,500
100-10,000
7.8 x 300 mm
7.8 x 300 mm
WAT085500
WAT085501
WAT010570
WAT010571
Fermentation Analysis, Organic Acids, Alcohols, and Carbohydrates

T he ion-exclusion mode is ideally suited for the separation of monosaccharides, organic acids, or sugar acids. T he column packings are sulfonated
styrene divinylbenzene resins in the hydrogen form (IC-Pak Ion Exclusion or
SH-1011), and the mobile phase is a dilute acid such as 0.01N H3P04 at
50-60 C.
Using this mode, the Fast Fruit Juice column can effectively separate
glycerol, acetic acid, and ethanol in grape or other fruit juices. T he
degree of microbial defect, the extent of natural fermentation in grapes
and the amount of sulfite present in various foods and beverages can also
be analyzed with the Fast Fruit Juice column. T he IC-Pak Ion-Exclusion
column can separate a wide range of organic acids while the Shodex SH
column separates acids as well as larger carbohydrates.
T he analysis of alcohols and organic acids is important as these compounds
typically play a role in determining the flavor characteristics of beverages
such as wine, beer and some spirits. T he presence of alcohols in fruit juices
can indicate product deterioration. T he Shodex KC-811 column is packed
with a sulfonated rigid styrene divinylbenzene copolymer providing high
efficiency separations of low molecular weight organic acids and water
soluble organics suc h as alcohols, aldehydes and nitriles. T he column
provides ion exclusion and reversed-phase mode of chromatography. Typical
mobile phases are aqueous solutions containing 1% phosphoric, acetic or
perchloric acid run 1 mL/min at 45-80 C.
www.waters.com
KC-811 Column Retention Chart for Organic Acids

Eluent: Water with 0.1% Phosphoric Acid, Temperature: 60 C, Flow rate: 1 mL/min
Sample
Oxalic Acid
Maleic Acid
a- Ketoglutaric Acid
Citric Acid
Tartaric Acid
Pyruvic Acid
trans-Aconitic Acid
Glyoxylic Acid
Malic Acid
Malonic Acid
Citraconic Acid
Succinic Acid
Glycolic Acid
Itaconic
Lactic Acid
Retention Time
5.20
5.80
5.90
6.20
6.55
6.65
6.95
7.00
7.05
7.07
7.20
8.00
8.40
8.50
8.60
Column
Fast Fruit Juice Analysis
Fast Fruit Juice Guard-Pak Inserts 10/pk
SH-1011
SH-1011P pre-column
KC-811
KC-811 pre-column
Sample
Retention Time
- Hydroxypropionic Acid
D-Glucuronic Acid
Fumaric Acid
Formic Acid
Acetic Acid
Adipic Acid
Levulinic Acid
Mesaconic Acid
Pyroglutamic Acid
Propionic Acid
Acrylic Acid
Pivalic Acid
Methacrylic Acid
trans-Crotonic Acid
8.60
8.65
8.95
9.20
9.80
9.80
10.00
10.40
10.70
11.25
11.60
14.05
14.10
15.65
Dimension
Part No.
7.8 x 150 mm
WAT010639
WAT015207
WAT010290
WAT034236
WAT034243
WAT034298
WAT035501
7.8 x 300 mm
8 x 300 mm
6 x 50 mm
8 x 300 mm
6 x 50 mm
173
Ion Analysis
Waters offers an array of products for ion c hromatography as well as
innovative capillary electrophoresis products for ion analysis (see the
Capillary Electrophoresis section). In ion c hromatography, anions and
cations are typically measured in two separate analyses. T he columns
offered for eac h type of analysis are briefly described below.
IC-Pak Anion Column

Column:
Part Number:
Sample:
Eluent:
Detection:
Waters IC-Pak resin-based columns allow you to analyze a full range of

ions from numerous sample matrices, both simple and complex. T hey offer
an exceptional linear loading range of less than 1.0 ppb to greater than
400 ppm without dilution and without pH limitations on eluent or sample.
T he flexibility exists for accurate and reproducible anion and cation analyses at all concentration levels. T he IC-Pak series of resin-based columns
shares the same chemistry and gives you identical elution order profiles.
T he IC-Pak Anion series of columns is used for the analysis of inorganic
anions, while the IC-Pak Ion-Exclusion columns are used for organic
acid analysis.
T he selection of a cation analysis column depends on the type of cation
being measured. T he IC-Pak C M/D column separates alkali and alkaline
earths more efficiently than the traditional IC-Pak Cation column. T he
IC-Pak C M/ D column also separates ethanolamine-related organic
cations. Transition metals can be separated using a Delta-Pak C18 column.
T he lanthanide series can be separated using Resolve C18 (a nonendcapped C18 material). Finally, metallocyanides can be analyzed on a
Nova-Pak C18 column.
IC-Pak Anion, 4.6 x 50 mm

WAT007355
Seven anion standard
Borate/gluconate
Waters 431 Conductivity Detector
1.
2.
3.
4.
5.
6.
7.
8.
Fluoride
Bicarbonate
Chloride
Nitrite
Bromide
Nitrate
Phosphate
Sulfate
1ppm
2ppm
4ppm
4ppm
4ppm
6ppm
5ppm
3
4
1
6
5
8
7
2
15 Min.
The IC-Pak Anion column is a configuration of 10 m anion exchange packing material

and a short column length which makes this the column of choice for rapid routine analyses.
IC-Pak Anion HR Column

Column:
Part Number:
Sample:
Eluent:
Detection:
IC-Pak Anion HR, 4.6 x 75 mm

WAT026765
Borate/gluconate
Waters 431 Conductivity Detector
3
5
1
IC-Pak Anion HC Column
1.
2.
3.
4.
5.
6.
7.
8.
Fluoride
Bicarbonate
Chloride
Nitrite
Bromide
Nitrate
Phosphate
Sulfate
1ppm
2ppm
4ppm
4ppm
4ppm
6ppm
5ppm
7
2
Column:
IC-Pak Anion HC column,

4.6 x 150 mm
WAT026770
Borate/gluconate
Waters 431 Conductivity
Detector
Part Number:
Sample:
Eluent:
Detection:
1.
2.
3.
4.
5.
6.
7.
8.
Fluoride
Bicarbonate
Chloride
Nitrite
Bromide
Nitrate
Phosphate
Sulfate
1ppm
2ppm
4ppm
4ppm
4ppm
6ppm
5ppm
15 Min.
IC-Pak Anion HR (high resolution) columns are packed with smaller 6 m particles.
This column is designed for the most demanding separations where the columns high efficiency
gives enhanced resolution and outstanding sensitivity.
2
1
6
7
IC-Pak Anion Analysis Columns

A family of anion exchange columns with different characteristics has been
developed to meet the needs of even the most demanding separations.
22 Min.
IC-Pak Anion HC (high capacity) columns use the same packing material as the IC-Pak Anion columns
and are best suited for applications where there are orders of magnitude differences in the concentrations
of the analytes. This is often the case with water and soil samples where you may have to measure ppb levels of
phosphate in the presence of ppm levels of chloride and nitrate. The IC-Pak Anion HC column gives
excellent resolution, even with injection volumes up to 500 L.
Description
IC-Pak Anion
IC-Pak Anion HR
IC-Pak Anion HC
IC-Pak Anion Guard-Pak Kit
(Guard-Pak Holder and 5 inserts)
IC-Pak Anion Concentrator Inserts*, 5/pk
IC-Pak Anion Guard-Pak Inserts*, 5/pk
Guard-Pak Holder
Dimension
Part No.
4.6 x 50 mm
4.6 x 75 mm
4.6 x 150 mm
WAT007355
WAT026765
WAT026770
WAT007357
WAT007358
WAT010551
WAT088141
* Requires Guard-Pak Holder
174
www.waters.com
Ion Analysis
IC-Pak Cation Analysis Columns
T he silica-based IC-Pak C M/D column allows a simultaneous analysis of

monovalent and divalent cations under isocratic conditions. T his technology
provides the analytical chemist with the most sensitive means of analyzing
monovalent cations such as Na+, K+, Li+ and NH4+.
T he IC-Pak Cation column is packed wit h 10 m sulfonated styrene
divinylbenzene particles. Monovalent and divalent cations may be analyzed
in two separate runs. Different mobile-phase compositions are used for the
two analyses.
Part Number:
Eluent:
Flow Rate:
Injection:
Detection:
IC-Pak C M/D column

IC-Pak C M/D Guard-Pak inserts*, 10/pk
IC-Pak Cation column
IC-Pak Cation Guard column
IC-Pak Cation Concentrator inserts*, 5/pk
Dimension
Part No.
3.9 x 150 mm
WAT036570
WAT044250
WAT007354
WAT007356
WAT010565
4.6 x 50 mm
4.6 x 50 mm
Ion Exclusion Columns
IC-Pak C M/D
Method:
Column:
Description
Waters IC-Method C-207

Waters IC-Pak C M/D,
3.9 x 150 mm
WAT036570
0.1 mM EDTA/3.0 mM Nitric Acid
1 mL/min
100 L
Conductivity
Cation
1. Lithium
2. Sodium
3. Ammonium
4. Potassium
5. Magnesium
6. Calcium
1
23
Waters IC-Pak Ion Exclusion columns are used for the analysis of
weak acid anions such as fluoride and short chain organic acids from
formate to butyrate.
4
5
Description
IC-Pak Ion Exclusion column
IC-Pak Ion Exclusion column
IC-Pak Ion Exclusion Guard-Pak inserts*, 10/pk
Guard-Pak Holder
Standards
0.54 ppm
1.0 ppm
2.0 ppm
4.0 ppm
2.0 ppm
3.0 ppm
Dimension
Part No.
7.8 x 150 mm
7.8 x 300 mm
WAT010295
WAT010290
WAT020770
WAT088141
* Requires Guard-Pak Holder

14 Min.
Transition Metal Analysis
Lanthanide Analysis
Transition metals can be separated on a dynamically coated C18 column

and detected at low ppb levels using post-column addition of PAR reagent
with UV detection. T his method provides excellent resolution with good
selectivity and analysis time.
Lanthanide Analysis Using a Resolve C18 Radial-Pak Cartridge
Transition Metal Analysis using Post-Column Derivatization

Column:
Part Number:
Injection:
Flow Rate:
Eluent:
Post-column
Reagent:
Part Number:
Sample:
Injection:
Eluent A:
Eluent B:
Delta-Pak C18, 5 m, 100,

3.9 x 150 mm
WAT011795
100 L
0.8 mL/min
2 mM Sodium Octanesulfonate, 35 mM
Sodium Tartrate, 5% Acetonitrile, adjust
to pH 3.65 with NaOH
0.2 mM PAR [4-(2-pyridylazo)resorcinol],
1 M Acetic Acid,
3 M Ammonium Hydroxide, 0.5 mL/min
1
Column:
Working Standard
1. Cu
=
2. Pb
=
3. Zn
=
4. Ni
=
5. Co
=
6. Cd
=
7. Fe(II) =
8. Mn 8 =
Flow Rate:
Post-column
Reagent:
0.5 ppm
1.0 ppm
0.5 ppm
1.0 ppm
0.5 ppm
1.0 ppm
2.0 ppm
1.0 ppm
Detection:
Resolve C18, 8 x 100 mm

Radial-Pak cartridge
WAT084624
Lanthanides
10 L
10 mM sodium
octanesulfonate, 100 mM
hydroxyisobutyric acid, pH 3.8
10 mM sodium
octanesulfonate, 400 mM
hydroxyisobutyric acid, pH 3.8
2.0 mL/min
Delta-Pak C18
Dimension
3.9 x 150 mm
Particle
Size
5 m
10
11
12 13
14
Lu
Yb
Tm
Er
Ho
Dy
Tb
8.
9.
10.
11.
12.
13.
14.
Gd
Eu
Sm
Nd
Pr
Ce
La
4
3
2
1
17 Min.
20 Min.
Description
0.13 mM arsenazo III,

10 mM urea, 62 mM
acetic acid at 1 mL/min
UV at 658 nm
1.
2.
3.
4.
5.
6.
7.
Working Standard
2 ppm of each
Pore
Size
100
Part No.
WAT011795
Description
Dimension
Resolve C18 *
8 x 100 mm
8 x 10 Cartridge Holder
Particle
Size
Pore
Size
5 m
90
Part No.
WAT084624
WAT082887
* Requires 8 x 10 Cartridge Holder
www.waters.com
175
Gas-Chromatography Packings
Waters versatile Porapak gas-c hromatography column packing materials simplify the analysis of a wide variety of complex compounds from
atmospheric gases to organics. Consisting of polymer beads, these unique
packings are chemically and physically stable and have consistent particle
size, porosity and surface area to guarantee analytical reproducibility. T hey
also provide unequaled separation capability with high resolution and low,
constant retention volumes.
Versatility for Specialty Applications

To optimize separation of even the most complex matrices, several physical
and chemical variations are available within the Porapak packing materials.
Special characteristics of Waters unique GC packings include:
Fast analysis with compounds eluting in distinctive

bands with no tailing
T he ability to sustain elevated temperatures permitting

temperature programming without adverse effects to
retention, reproducibility, and column life
T he ability to accommodate large sample loads required

for preparative and trace analysis while maintaining
c haracteristically high column efficiency
GC Porapak Porous Polymer Packing

Surface Area
(m2/g)
Density
(g/cm3)
Single Temp
Prog.
Particle Size
Mesh
Qty.
Part No.
Nonpolar
100-200
0.26
250 C
PS
Nonpolar
100-200
0.26
250 C
Slightly nonpolar
to moderate
500-600
0.34
250 C
QS
Slightly nonpolar
to moderate
500-600
0.34
250 C
Moderate
Polar monomer:
vinyl pyrrolidone
Moderate
Polar monomer:
vinyl pyridine
Polar
Polar monomer:
vinyl pyrrolidone
Highly polar
Polar monomer:
ethyleneglycol
dimethacrylate
450-600
0.32
250 C
300-450
0.35
250 C
250-350
0.41
190 C
225-350
0.39
190 C
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
20 g
20 g
20 g
20 g
20 g
20 g
26 g
26 g
26 g
26 g
26 g
26 g
24 g
24 g
24 g
26 g
26 g
26 g
29 g
29 g
29 g
31 g
31 g
31 g
WAT027053
WAT027054
WAT027055
WAT027083
WAT027084
WAT027085
WAT027059
WAT027060
WAT027061
WAT027089
WAT027090
WAT027091
WAT027065
WAT027066
WAT027067
WAT027071
WAT027072
WAT027073
WAT027047
WAT027048
WAT027049
WAT027077
WAT027078
WAT027079
Type
Polarity
176
www.waters.com
PREPaRaTIVE ChROMaTOGRaPhY
Column PerformanceIdentifying the Problem

Laboratory-scale HPLC purification presents many c hallenges to the
c hromatographer. One of the most frustrating c hallenges relates to the
preparative column itself. Inconsistencies in column-to-column performance
and lifetimes often result in lost samples, repeat purification runs, and
poor scalability from small to large volume columns. Scientists at Waters
recognized this problem and, over a 3-year period, studied all aspects of
the packing processes as well as the column design. Launched in 2003, the
Waters proprietary* Optimum Bed Density (OBD) design is a direct result of
these investigations.
For a column to remain stable during operation, the bed must be packed
sufficiently dense to withstand the compressive fluid forces encountered
during use. In the case of analytical column dimensions, the necessary
packed bed density can generally be ac hieved using traditional slurry
packing methods. As the diameter and length of the column increases, it
becomes increasingly difficult to reach the bed density required for stable,
long-term performance when using small particles. Optimizing the bed
density depends on the specific properties of the chromatographic particles
and column design being used.
Average Bed Density for Conventional Analytical-LC-ColumnSlurry-Packing Procedures vs. Column Aspect Ratio
Packed-Bed Density
[g/cm3]
0.700
Optimum Packed-Bed Density
0.675
4.6 x 100 mm
Analytical LC Columns
0.650
19 x 50 mm
0.625
Standard Packing Procedures Insufficient

to Achieve Optimum Packed-Bed Densities
in Larger-Diameter Columns
19 x 100 mm
0.600
50 x 100 mm
Prep LC Columns
0.575
0.550
0
10
15
20
25
Column Aspect Ratio Length/Diameter
* US Patent # 7,399,410, UK Patent # GB2408469
Waters OBD Column Design

Waters has combined high-pressure slurry packing with a carefully calculated axial compression element localized at the less-dense inlet end of the bed.
High-pressure Slurry Packing

Localized Bed Expansion Near Inlet
Axial-ram Slurry Packing

Localized Compression Near Inlet
OBD Process
Optimum Bed Density Throughout
Optimum Bed Density
Packed-bed
Density
Axial-ram-compressed
Slurry Packed
High-pressure
Slurry Packed
Column Inlet
Optimized bed density in an OBD column is achieved by judicious

application of a tuned amount of axial force only at the column inlet.
With careful tuning of the packing process for eac h particle type and
column geometry, the Prep OBD design and process results in predictable,
uniform density profiles throughout the column. During the final capping
process, Waters Column Packing Operation follows carefully established
procedures designed not to over-compress or disrupt, in any non-uniform
way, this portion of the bed. Waters scientists have established that too
Column Outlet
Effect of packing method on average bed

density across column length.
muc h axial compression applied at the inlet can break particles, build
bridges, and lower local bed permeability.
T he Prep OBD column is designed to incorporate a pair of speciallydesigned distributors and chemically-inert seals made to prevent leaks at
high operating pressures.
An exploded view of the elements

of an empty OBD column.
www.waters.com
177
Column Stability and ReliabilityLong, Predictable Lifetimes

T he demand for rapid high purity compound isolation places strong
emphasis on the integrity and stability of the preparative column.
Complex, sparingly-soluble starting materials are often dissolved with
strong solvents, suc h as DMSO. T his combination of poor solubility
and pressure shocks associated with large injection volumes of pure
organic solvent are the primary contributors to early column failure and
c hromatographic bed collapse.
T he OBD design exhibits exceptional resistance to mechanical chromatographic bed failure and delivers consistent column-to-column performance,
reducing cost through extended lifetimes.
Data from a High-throughput Drug Discovery Laboratory: 7,000 Injections on an XBridge Prep OBD C18 Column, 19 x 50 mm, 5 m
Injection 1
Injection 7,000
ScalabilityAnalytical Performance in a Prep OBD Column

Successful scaling of methods from analytical to preparative dimensions
requires the use of chromatographically equivalent columns. All too often,
even when the same chemistry phase and particle size are used, methods
do not scale either due to loss of resolution and/or lower than expected
loading. By matching the analytical and preparative column bed densities,
scalability is assured, eliminating the need for any time consuming method
re-development.
Accurate Scale-up on SunFire Columns in 0.1% TFA

Column:
Mobile Phase A:
Mobile Phase B:
Temperature:
Detection:
Instrument:
100
OBD preparative columns are packed to bed densities which closely match
the equivalent analytical column. T his innovative procedure produces
preparative columns with excellent stability, reproducibility, and efficiency.
SunFire C18
0.1% TFA in Water
0.1% TFA in ACN
Ambient
UV @ 260 nm
Waters AutoPurification System
Compounds
1. Nadolol (100 mg/mL)
2. Metoprolol (100 mg/mL)
3. Propranolol (50 mg/mL)
4.6 x 50 mm
Total Mass Load: 5 mg
Flow Rate:
Gradient:
1.4 mL/min
Time
(min)
0.0
1.0
6.0
7.0
20 L
Inj. Vol.:
Profile
%A
95
95
30
30
%B
5
5
70
70
-2
100
19 x 50 mm
Flow Rate:
Gradient:
23.9 mL/min
Time
Profile
(min)
%A
0.00
95
1.79
95
6.79
30
7.79
30
340 L
Inj. Vol.:
%B
5
5
70
70
-2
1
178
6 Min.
www.waters.com
Selecting the Column Packing

Waters column packing materials bring you the best in preparative chromatography options. For optimum flexibility, our column packing materials are
available in a range of steel columns. A complete list of preparative packing
materials and their characteristics is shown in the following table.
Preparative Packing Characteristics
Chemistry
Particle
Size
Pore
Size
Particle
Shape
Carbon
Load
Endcapped
XSelect
C18
Fluoro-Phenyl
Phenyl-Hexyl
5 m
5 m
5 m
130
130
130
Spherical
Spherical
Spherical
15%
10%
14%
Yes
Yes
Yes
XBridge
C18
C8
Shield RP18
Phenyl
HILIC
5, 10 m
5, 10 m
5, 10 m
5 m
5 m
130
130
130
130
130A
Spherical
Spherical
Spherical
Spherical
Spherical
18%
13%
17%
15%
N/A
Yes
Yes
Yes
Yes
No
Packing
SunFire
C18
C8
Silica
5, 10 m
5, 10 m
5, 10 m
100
100
100
Spherical
Spherical
Spherical
16%
12%
N/A
Yes
Yes
Yes
Atlantis
T3
dC18
HILIC
5, 10 m
5, 10 m
5 m
100
100
100
Spherical
Spherical
Spherical
14%
11%
0%
Yes
Yes
No
XTerra MS
C18
C8
5, 10 m
5, 10 m
125
125
Spherical
Spherical
16%
14%
Yes
Yes
XTerra RP
C18
C8
5, 10 m
5, 10 m
125
125
Spherical
Spherical
15%
14%
Symmetry
C18
C8
5, 7 m
5, 7 m
100
100
Spherical
Spherical
SymmetryShield
C18
C8
5, 7 m
5, 7 m
100
100
Symmetry300
C18
C4
5, 7 m
5, 7 m
300
300
Particle
Size
Pore
Size
Particle
Shape
Carbon
Load
Endcapped

Silica
6 m
6 m
60
60
Spherical
Spherical
7%
N/A
Yes
N/A
Bondapak
C18
Phenyl
CN
NH2
10 m
10 m
10 m
10 m
125
125
125
125
Irregular
Irregular
Irregular
Irregular
10%
8%
6%
4%
Yes
Yes
Yes
No
Bondapak
C18
15-20 m
37-55 m
125
125
Irregular
Irregular
10%
10%
Yes
Yes
Bondapak HC18 HA C18
37-55 m
125
Irregular
14%
Yes
Packing
Chemistry
Porasil
Silica
10 m
125
Irregular
N/A
N/A
Yes
Yes
Porasil
Silica
15-20 m
37-55 m
125
125
Irregular
Irregular
N/A
N/A
N/A
N/A
19%
12%
Yes
Yes
Delta-Pak
C18
15 m
100
300
Spherical
Spherical
17%
7%
Yes
Yes
Spherical
Spherical
15%
17%
Yes
Yes
Delta-Pak
C4
15 m
100
300
Spherical
Spherical
7%
3%
Yes
Yes
Spherical
Spherical
9%
3%
Yes
Yes
Prep
C18
Silica
55-105 m
55-105 m
125
125
Irregular
Irregular
11%
N/A
Yes
N/A
No matter how you collect fractions, whether by mass spec, UV, ELS, or
other detector signals, the Waters AutoPurification systems will meet
your needs. T hese systems provide simplicity, scalability, and speed with
a variety of available configurations. T he market leading FractionLynx
Application Manager provides an intuitive interface to manage the
purification process from sample acquisition to reporting.
www.waters.com
Pictured here is the Waters AutoPurification system with a 2767 Sample

Manager for inject/collect/re-inject, 2545 Binary Gradient Module for
high pressure mixing, System Fluidics Organizer for software-controlled
valving, fluidics, and column management, 2998 PDA, 2424 ELS and
3100 Mass Detectors.
179
Choosing Preparative Columns
Mass Loading
Step 1
Once the analytical separation has been optimized, a loading study on
the analytical column is performed to determine the capacity of the
particular packing material. Because the large-scale separation should
be identical to the small scale separation, the maximum sample load
will be dependent upon the complexity of the analytical separation.
Approximate Mass Loading Capacity (mg)

for Prep OBD Columns (Gradient Mode)
Length (mm)
30
50
75
100
150
250
4.6
5
8
13
10
15
25
40
60
Diameter (mm)
19
27
45
90
135
225
30
110
165
225
335
560
50
310
620
930
1550
Reasonable Flow Rate (mL/min)

Reasonable Injection Volume (L)
1.4
20
6.6
100
24
350
60
880
164
2450
Step 2
Determine how much mass you need to purify or isolate.
Step 3
Once the desired purified mass is established, some simple equations
may be used to determine the required column size for purification. In
addition, preparative HPLC system maximum flow rate and back pressure
need to be considered and can limit column size.
T he calculated prep gradient duration is entered into the pumps gradient separation
over the same number of column volumes as was used in the analytical run.
Many factors affect the mass capacity of preparative columns. T he listed

capacities represent an average estimate.
Scale-up factor
Capacity is:
(Diameter prep)2 x Length prep

Scale-up factor =
(Diameter analytical)2 x Length analytical
Consider scaling up from a 4.6 x 150 mm column to a 19 x 150 mm

column:
(19)2 x 150
Scale-up factor=
Higher for strongly retained material
Higher for simple mixtures
Lower where higher resolution is required
Very strongly dependent on loading conditions

Limited by loading volume
= 17.1
(4.6)2 x 150
Limited by diluent solvent strength
Applying the scale-up factor, we can predict that approximately 17-135 mg

of sample could be applied to the larger column (packed with the same
material as the analytical column). T his range is based on an analytical
(4.6 mm i.d.) mass load of 1-8 mg.
Flow Rate
(Diameter prep)2
Flow rate (prep) = Flow rate (anal) x
Particle Size (anal)

x
(Diameter anal)2
Particle Size (prep)
T he calculated flow rate may be used for the larger column to ensure
the same linear velocity of mobile phases as used in the analytical run.
However, reasonable flow rates are based on column diameters. Systems
will be limited by increasing backpressure with increasing column
length and decreasing particle size.
Gradient Duration (GD)
GD (prep) =
GD (anal) x Length (prep)

Length (anal)
Reasonable flow rates are based on column diameter. Systems will be

limited by increasing backpressure with increasing column length and
decreasing particle size.
Reasonable injection volumes are based on column diameter at a length of
50 mm with relatively strong solvents. Increased length is compatible with
larger injection, but not proportionately so. Weaker solvents significantly
increase injection volume.
Mass loading capacities for peptide purifications depend strongly on the
sequence and may be estimated at 5-20% of listed values.
Preparative OBD Column Calculator

Designed to simplify common preparative calculations:
Diameter (prep)
Diameter (anal)2
Flow rate (anal)

Flow rate (prep)
Column/tubing backpressure
Mass load scale-up
Gradient scale-up
Gradient designs
Register online at www.waters.com/prepcalculator

and download your FREE copy
180
www.waters.com
NEW XSelect Prep OBD Columns
Columns Designed for Isolation and Purification
CSH Tec hnology, especially when combined with the analytical method
development capabilities of UPLC Tec hnology and the industry-leading
column lifetimes offered by Optimum Bed Density (OBD) preparative
columns, may have the greatest benefits to isolation and purification scientists. W hy? CSH Technology offers the highest loading of basic compounds
separated under the volatile, acidic, low pH mobile-phase conditions often
preferred by isolation and purification scientists. T his increased loadability
allows for the use of a narrower (lower volume) preparative column thereby
reducing solvent consumption and fraction volumes.
T he scalability built into ACQUITY UPLC CSH and XSelect columns permits
the use of UPLC Technology as a powerful method development tool for the
isolation and purification scientist. How can c hromatographic separation
platforms that appear to be on opposite ends of the analysis scale spectrum
(microbore vs. preparative) complement eac h other? Again, the answer
lies in scalability and CSH Technologys resistance to pH switching column
performance degradation. Fast, robust, analytical-scale separations
can be easily and quickly developed using UPLC Tec hnology, ACQUIT Y
UPLC CSH columns and simple mobile-phase additives and then straightforwardly scaled and transferred to an XSelect OBD preparative column.
In addition, the purity check of the isolated fraction(s) and/or structural
analysis of isolated impurities can be determined using UPLC/MS.
No other manufacturer can offer the isolation and purification scientist
t he selectivity, loadability and scalability of CSH Tec hnology wit h
the throughput, sensitivity and transferable analytical-scale method
development of UPLC Tec hnology.
Literature
References
ACQUITY UPLC CSH and XSelect HPLC
Columns Brochure, Literature Reference
720003495EN
Charged Surface Hybrid (CSH) Technology and
Its Use in Liquid Chromatography W hite Paper,
Practical Applications of Charged Surface
Hybrid (CSH) Technology Application Note,
BEH Technology W hite Paper, Literature
Reference 720001159EN
www.waters.com
Optimum Bed Density (OBD) Columns:

Enabling Technology for Laboratory-Scale
Isolation and Purification W hite Paper,
181
PREPARATIVE CHROMATOGRAPHY
LC Conditions
Columns:
Part Number:
Mobile Phase A:
Mobile Phase B:
Gradient:
Compounds
1. Diphenhydramine
2. Oxybutynin
3. Terfenadine
ACQUITY UPLC CSH C18, 2.1 x 50 mm, 1.7 m

186005296
1.50
0.1% formic acid in water
0.1% formic acid in acetonitrile
Time
Flow
Profile
1.00
(min)
(mL/min) %A
%B
Curve
Initial
0.9
95
5
Initial
2.0
0.0
5
95
6
0.50
2.5
0.9
5
95
6
2.51
0.9
95
5
6
3.50
0.9
95
5
6
0.00
1 L
800 g/mL for crude mix
0.0
50/50 methanol/water
40 C
95/5 water/acetonitrile
2.00
UV @ 220 nm
20 points/sec
1.50
0.1 sec
ACQUITY UPLC with
1.00
ACQUITY UPLC PDA
Target Analyte
1
AU
Crude mix
Diphenhydramine = 56.3% purity
1.50
Target Analyte
1
3
0.2
0.4
0.6
0.8
1.0
AU
1.00
1.2
1.4
1.6
1.8
2.0 min
3. Run preparative separation using focused gradient

0.50
Collected Fraction
0.00
0.0
0.2
AU
0.00
4
0.6
0.8
1.00
1.50
0.00
3
0.50
10 min
Crude mix
Diphenhydramine = 56.3% purity
0.00
0.0
0.2
0.4
0.6
0.8
1.0
AU
AU
1.00
Pooled purified fractions

Diphenhydramine = 100% purity
0.50
1.2
1.4
1.6
1.8
2.0 min
0.00
0.0
0.2
0.4
1
2.00
0.6
0.8
1.0
1.2
1.4
1.6
1. Develop method using

UPLC Technology
1.8
2.0 min
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0 min
5. Confirm fraction purity

using UPLC Technology
Collected Fraction
Using CSH Technology throughout the entire process, methods can be developed quickly with ACQUITY UPLC CSH columns and UPLC Technology and then transferred to preparative-scale
OBD XSelect columns for isolation and purification. The purity of the isolated fraction(s) can then be measured/confirmed using ACQUITY UPLC CSH columns and UPLC Technology.
1.50
AU

Diphenhydramine = 100% purity
0.00
1.00
0.50
XSelect Preparative
Columns
10 x 10 mm 1.50
10 x 50 mm
10 x 100 mm 1.00
10 x 150 mm
10 x 250 mm 0.50
19 x 10 mm
OBD 19 x 50 mm0.00
OBD 19 x 100 mm
0.0
OBD 19 x 150 mm
OBD 19 x 250 mm
OBD 30 x 50 mm
OBD 30 x 75 mm
OBD 30 x 100 mm
OBD 30 x 150 mm
OBD 30 x 250 mm
OBD 50 x 50 mm
OBD 50 x 100 mm
OBD 50 x 150 mm
OBD 50 x 250 mm
Particle6
Size
Type 4
Guard
Column
Column
Column
Column
Guard
Column
Column
0.2
0.4
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Prep Purification
100 mg total load
0.00
AU
1.50
AU
Target Analyte
1
0.50
182
XSelect CSH Prep C18 OBD, 19 x 100 mm, 5 m

186005421
Time
Flow
Profile
(min)
(mL/min) %A
%B
Curve
1.21
25
95
5
Initial
1.71
25
95
5
6
7.02
25
78.8
21.2
6
7.21
25
70.8
29.2
6
8.21
25
5
95
6
8.31
25
95
5
6
13.21 25
95
5
6
1.25 mL
80 mg/mL for crude mix
40 mg/mL
16 mg/mL
24 mg/mL
DMSO
40 C
95/5 water/methanol
95/5 methanol/water
UV @ 220 nm
1 point/sec
1 sec
Waters 2525 Binary Gradient Module,
2767 Sample Manager, Column Fluidics
Organizer, 2996 Photodiode Array
Detector, ZQ Mass Spectrometer
1.00
1.00
Dimension
Prep Purification
100 mg total load
4. Collect and isolate
fraction(s) of interest
1.50
Injection Volume:
Crude mix
DiphenhydramineSample
= 56.3%
purity
Concentration:
Diphenhydramine:
Oxybutynin:
Terfenadine :
1.2
1.4
1.6Sample
1.8Diluent:
2.0 min
Column Temperature:
Weak Needle Wash:
3
Strong Needle Wash:
Detection:
Sampling Rate:
System:
10 min
0.50
2. Scale UPLC separation

to preparative column scale
1.0
Collected Fraction
Prep Purification
100 mg total load
1.50
0.4
1
2.00
0.50
AU
Injection Volume:
Sample Diluent:
Column Temperature:
Weak Needle Wash:
Strong Needle Wash:
Detection:
Sampling Rate:
System:
Preparative Conditions
Column:
Part Number:
Mobile Phase A:
Mobile Phase B:
Gradient:
0.6
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
0.8
1.0
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
Fluoro-Phenyl
Phenyl-Hexyl
1860054911
1860054981
186005414
186005427
186005415
186005428
186005416
186005429
186005417
186005430
Diphenhydramine
= 100% purity
1860054182
1860054312
186005420
186005433
186005421
186005434
1.2
1.4
1.6
1.8
2.0 min
186005422
186005435
186005492
186005499
186005423
186005436
186005424
186005437
186005425
186005438
186005426
186005439
186005493
186005500
186005494
186005501
186005495
186005502
186005496
186005503
186005497
186005504
1860055051
186005440
186005441
186005442
186005443
1860054442
186005446
186005447
186005448
186005506
186005520
186005450
186005451
186005452
186005507
186005508
186005509
186005510
186005511
C818
10 min
Description
1
2
Part No.
10 x 10 mm Cartridge Holder
289000779
186000709
Replacement o-ring 7.8 mm, 2/pk

Replacement o-ring 10 mm, 2/pk
700001019
700001436
700001020
Requires 10 x 10 mm Prep Guard Holder, Part No. 289000779

www.waters.com
XBridge Prep OBD Columns
Bridging the Performance Gap from Analytical to Prep
A Particle Designed for Purification
Preparative packing materials are routinely offered with a muc h wider
particle size distribution than the equivalent analytical particle. W hile this
approach does reduce the cost of material manufacturing, the chromatographic performance is almost certainly compromised.
XBridge Prep packing materials are fully optimized to deliver maximum
efficiency and the highest loadability at the lowest possible backpressures.
T hese attributes allow the purification chemist the flexibility to choose the
best combination of particle size and column dimension to easily purify
even the most complex of samples.
A Milestone in Chromatography
First introduced in 1999, Waters patented Hybrid Particle Technology (HPT)
surmounted significant limitations of silica-based, reversed-phase packing
materials, particularly their hydrolytic instability at high pH. In 2005, the
introduction of a second generation HPT, branded as XBridge Prep columns
marks a new milestone in chromatography.
With an order of magnitude improvement in high pH stability and a higher
level of chromatographic performance, XBridge Prep columns define the
new benchmark for LC purification.
Maximum Efficiency/30% Lower Backpressure

Mobile Phase A:
Mobile Phase B:
Sample
Concentration:
Instrument::
Detection:
0.1% DEA in water

0.1% DEA in acetonitrile
300 mg/mL in DMSO
UV @ 260 nm
XTerra Prep MS C18, 19 x 50 mm, 5 m

Injection Volume: 660 L
Total Load: 198 mg
Maximum
3
Backpressure: 1000 psi
Gradient:
Time
(min)
0.0
1.79
6.79
7.79
Profile
%A
%B
95
5
95
5
5
95
5
95
Gradient:
Time
(min)
0.0
1.79
6.79
7.79
Profile
%A
%B
95
5
95
5
5
95
5
95
5
1
XBridge Prep C18 and C8

C18 and C8 phases remain to be the workhorse of the purification chemist.
T he second generation HPT phases deliver not only unmatched pH stability,
but also an equivalent chromatographic performance and peak efficiency
to that of the best silica based product. A testament to the enhanced mass
transfer characteristics of this particle. T hese attributes, combined with the
OBD packing design, make the XBridge Prep C18 and C8 phases ideal for
robust purification procedures.
Compounds
1. Labetolol (50 mg/mL)
2. Quinine (50 mg/mL)
3. Diltiazem (50 mg/mL)
4. Verapamil (100 mg/mL)
5. Amitriptyline (50 mg/mL)
XBridge Prep C18, 19 x 50 mm, 5 m

Total Load: 198 mg
Maximum
Backpressure: 700 psi
XBridge Prep RP18

T he XBridge Prep RP18 phase combine the HPT process with the Waters
patented Shield Tec hnology. T his embedded polar group reversed-phase
material delivers maximum peak efficiency and a unique alternate
selectivity, particularly useful in the isolation of phenolic analytes. An
additional feature of this phase is the ability to routinely operate in
100% aqueous mobile phases without exhibiting the problems associated
with phase dewetting.
XBridge Prep Phenyl
P henyl c hromatographic phases have always been of interest to t he
chromatographer due to the alternative selectivity, particularly with respect
to polyaromatic compounds. Unfortunately, routine use of these phases is
not common due to their inherent poor stability and reproducibility.
A combination of the second generation HPT and a tri-functional bonding
approach in the XBridge Prep Phenyl delivers, for the first time, a truly pH
stable and reproducible phase, ideal for drug purification.
www.waters.com
10
12
14
16
18
20 Min.
XBridge Prep Columns deliver the same high loading capacity and reliability expected
of our XTerra Prep products, with a significantly reduced column backpressure.
Literature
References
Waters XBridge HPLC Columns Brochure,
BEH Technology W hite Paper,

183
XBridge Column Characteristics

Chemistry
Ligand
Density
O
OO Si
OSi
OO
OO
OSi
OOSi
Trifunctional C18
Carbon
Load
pH
Range
Pore
Diameter
Surface
Area
3.1 mol/m2
18%
1-12
130
185 m2/g
3.2 mol/m2
13%
1-12
130
185 m2/g
3.3 mol/m2
17%
2-11
130
185 m2/g
Description
3.0 mol/m2
15%
1-12
130
185 m2/g
289000779
186000709
n/a
unbonded
1-9
130
185 m2/g

700001019
700001436
700001020
O
OO Si
O
OO Si
OO
O Si
OO Si
Trifunctional C8
Purification and Isolation Cartridge Holders
CH 3
Monofunctional Embedded
Polar Group (Shield RP18)
CH 3
O Si
CH 3
Si
CH
CH33
Si
CH3
Si
CH3
CH3
O
O
O
Polar Group
Polar Group
Polar Group
Trifunctional C6 Phenyl
Part No.
Polar Group
OO Si
OSi
OO
OO
OSi
OOSi
O
Unbonded BEH
XBridge Prep OBD Columns

Type
Particle
Size
C18
10 x 10 mm
10 x 50 mm
10 x 100 mm
10 x 150 mm
10 x 250 mm
19 x 10 mm
OBD 19 x 50 mm
OBD 19 x 100 mm
OBD 19 x 150 mm
OBD 19 x 250 mm
OBD 30 x 50 mm
OBD 30 x 75 mm
OBD 30 x 100 mm
OBD 30 x 150 mm
OBD 30 x 250 mm
OBD 50 x 50 mm
OBD 50 x 100 mm
OBD 50 x 150 mm
OBD 50 x 250 mm
Guard
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
10 x 10 mm
10 x 150 mm
10 x 250 mm
19 x 10 mm
OBD 19 x 50 mm
OBD 19 x 100 mm
OBD 19 x 150 mm
OBD 19 x 250 mm
OBD 30 x 75 mm
OBD 30 x 100 mm
OBD 30 x 150 mm
OBD 30 x 250 mm
OBD 50 x 50 mm
OBD 50 x 100 mm
OBD 50 x 150 mm
OBD 50 x 250 mm
Guard
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Dimension
1
2
C8
Shield RP18
Phenyl
HILIC
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186002972
186002973
186003255
186002974
186003256
1860029752
186002977
186002978
186002979
186004021
186002980
186002981
186002982
186003284
186004025
186003933
186003937
186003929
186004107
186002991
186003264
186003265
186003266
186003267
1860029922
186002993
186002994
186002995
186004023
186002996
186003269
186002997
186003083
186003934
186003938
186002983
186003257
186003258
186003259
186003260
1860029842
186002985
186002986
186002987
186004022
186002988
186003262
186002989
186002990
186003935
186003939
186003354
186003271
186003272
186003273
186003274
1860033552
186003356
186003357
186003358
186004024
186003277
186003278
186003279
186003276
186003936
186003940
1860047201
186004721
186004722
1860047232
186004724
186004725
186004726
186004730
186004727
186004728
186004729
186004731
186004732
186004733
186004734
186004735
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
1860038893
186003890
186003891
1860038924
186003893
186003901
186003894
186003895
186004711
186003930
186003896
186003897
186003898
186003902
186003899
186003900
1860040033
186004004
186004005
1860040064
186004007
186004008
186004009
186004010
186004011
186004012
186004013
186004014
186004015
186004016
1860039883
186003989
186003990
1860039914
186003992
186003993
186003994
186003995
186003996
186003997
186003998
186003999
186004001
186004002
For Purification
of Peptides, See
Pages 226-235

184
www.waters.com
SunFire Prep OBD Columns
Isolation of Nimodipine and Its Impurities
Mobile Phase A:
Mobile Phase B:
Analyte:
Detection:
Instruments:
Impurity levels:
Water with 0.1% FA

ACN with 0.1% FA
Nimodipine at 30 mg/mL in DMSO
UV @ 290 nm
Waters AutoPurification Systems
Flow Rate:
Gradient:
Nimodipine
100
SunFire C18
4.6 x 100 mm, 5 m
%
100
-1
%
SunFire columns set the standard for the state-of-the-art bonded C18
and C 8 silica HPLC columns. Benefiting from years of researc h and
product development, SunFire columns represent the best in particle
and bonding expertise and deliver the industry-leading level of
T he physicoc hemical c haracteristics of sorbents used for preparative

separations must be designed and/or c hosen not only to ac hieve the
selectivity necessary for optimal separations but also the load capacity that
enables maximum throughput. T his becomes especially tricky for ionizable
compounds in mobile phases with low to moderate pH. SunFire sorbents are
made from an engineered synthetic silica with proprietary surface-chemistry
modifications, all done under cGMP protocols, that extend loadability far
beyond that of competitive packings.
High Mass Loading of SunFire Sorbents Enables
the Use of Smaller Preparative Column Dimensions
Flow Rate:
Injection Volume:
Column:
Detection:
Instrument:
0.1% TFA in water

15 min linear gradient from 20% B to
85% B, 2 min initial holding time
1 mL/min
10 L
4.6 x 150 mm, 5 m
UV @ 254 nm
Alliance HT 2795
SunFire C18
Rs: 2.77
W1/2: 0.17
%
100
1
SunFire Prep OBD C18
19 x 100 mm, 5 m
-1
%
Kromasil C18
250 g
Rs: 1.19
W1/2: 0.53
mg/mL
25
50
50
125
mg/mL
20
40
40
100
mg/mL
10
20
20
50
mg/mL
5
10
10
25
Compounds:
1. Ketoconazole
2. Econazole
3. Miconazole
3
4
2
8
10
10
1.4 mL/min
Time
Profile
(min)
%A
0.00
70
1.00
70
11.00 10
12.00 10
12.10 70
16.00 70
200 L
23.9 mL/min
Time
Profile
(min)
%A
0.00
70
1.97
70
11.97 10
12.97 10
13.10 70
12
14 Min.
16.00 70
Inj. Vol.:
3.4 mL
%B
30
30
90
90
30
30
Flow Rate:
Gradient:
12
%B
30
30
90
90
30
30
14 Min.
SunFire Prep silica columns allow you to use normal-phase protocols. T his
gives you a significant selectivity alternative to reversed-phase preparative
c hromatography on bonded silicas. Analytes that are highly hydrophilic
or that are insoluble/unstable in water are ideal candidates for normalphase purification. Unlike the more viscous, higher-boiling aqueous-organic
mobile phases used in reversed phase, typical normal-phase eluents are
lower-viscosity, lower-boiling organic solvents. As a consequence, SunFire
Silica separations yield several key benefits:
High efficiency (plate counts similar

to those of analytical columns)
Excellent scale-up capability
Lower backpressure
Faster analyte recovery (rapid solvent-evaporation rates)
SunFire Prep columns, available in 5 m and 10 m particle sizes, exhibit

enhanced column life and stability due to the OBD design as well as
excellent peak shapes and high-mass load capacity.
Literature
References
500 g
Rs: 1.01
W1/2: 0.64
Rs: 1.92
W1/2: 0.26
1000 g
Rs: 0.81
W1/2: 0.90
SunFire Columns Brochure,

1250 g
Rs: 0.78
W1/2: 0.91

www.waters.com
Inj. Vol.:
Rs: 2.31
W1/2: 0.21
Rs: 1.87
W1/2: 0.27
% by Area
3.1
0.6
6.5
4.9
Normal-Phase SunFire Prep Silica Columns
As indicated in the table, dissolved in DMSO
Analyte
Ketoconazole
Econazole
Miconazole
Total Concentration
2
-1
Loadability by Design
Mobile Phase A:
Mobile Phase B:
Gradient:
100
-1
Peak
1
2
3
4

185
O
O
HO
O
O
O
O
O
HO
Purification of Diesters from Standard DCC/DMAP Protocol*

O
OH
O
Conditons
Column:
Part Number:
Mobile Phase:
Analytes:
1. Propyl p-phenylacetobenzoate
2. Methyl p-phenylacetobenzoate
3. Propyl paraben
4. Methyl paraben
SunFire Silica, 19 x 150 mm, 5 m

186002568
80/20 Hexane/
Ethyl acetate
18 mL/min
100 mg
Flow Rate:
Total Load:
O
O
O
OH
HO
Phenylacetic acid
Propyl paraben
O
O
Propyl p-phenylacetobenzoate
DCC/DMAP in THF
Methyl p-phenylacetobenzoate
Methyl paraben
O
O
HO
*A base-catalyzed (DMAP) esterification reaction, driven to completion using a

dehydrating agent (DCC). RCOOH + R'OH RCOOR' + H20
10
12 Min
SunFire 5 m Prep Columns
Dimension
10 x 50 mm
10 x 100 mm
10 x 150 mm
10 x 250 mm
OBD 19 x 50 mm
OBD 19 x 100 mm
OBD 19 x 150 mm
OBD 19 x 250 mm
OBD 30 x 50 mm
OBD 30 x 75 mm
OBD 30 x 100 mm
OBD 30 x 150 mm
OBD 30 x 250 mm
OBD 50 x 50 mm
OBD 50 x 100 mm
OBD 50 x 150 mm
OBD 50 x 250 mm
SunFire 10 m Prep Columns
Particle
Size
C18
C8
Silica
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186002561
186002562
186002563
186002564
186002566
186002567
186002568
186004027
186002570
186002571
186002572
186002797
186003969
186002867
186002869
186003941
186003970
186002746
186002747
186002748
186002749
186002751
186002752
186002753
186004028
186002755
186002756
186002757
186002795
186002868
186002870
186003425
186003426
186003427
186003428
186003431
186003432
186003433
186004029
186003435
186003436
186003437
186003438
186003439
186003440
SunFire Prep Guard Columns
Dimension
Particle
Size
C18
C8
Silica
10 x 50 mm
10 x 150 mm
10 x 250 mm
OBD 19 x 50 mm
OBD 19 x 150 mm
OBD 19 x 250 mm
OBD 30 x 50 mm
OBD 30 x 100 mm
OBD 30 x 150 mm
OBD 30 x 250 mm
OBD 50 x 50 mm
OBD 50 x 100 mm
OBD 50 x 150 mm
OBD 50 x 250 mm
OBD 100 x 250 mm
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
186003840
186002664
186002665
186002667
186002668
186002669
186003854
186003971
186002670
186002671
186002871
186003972
186002672
186002673
186003928
186003841
186002759
186002760
186002762
186002763
186002764
186003853
186002765
186002766
186002872
186002767
186002768
Custom
186003442
186003443
186003445
186003446
186003447
186003855
186003448
186003449
186003450
186003451
186003452
Dimension
Particle
Size
C18
10 x 10 mm
19 x 10 mm
10 x 10 mm
19 x 10 mm
5 m
5 m
10 m
10 m
186002565
1860025692
1860026631
1860026662
C8
1
Silica
186002750
1860027542
1860027581
1860027612
1
1860034291
1860034342
1860034411
1860034442
Description

1
2
SunFire Prep Scouting Columns
Dimension
Particle
Size
C18
C8
Silica
4.6 x 150 mm
4.6 x 250 mm
4.6 x 150 mm
4.6 x 250 mm
5 m
5 m
10 m
10 m
186003390
186003391
Custom
Custom
186003453
186003454
186003467
186003468
186
DMAP = 4-Dimethylaminopyridine; DCC = 1,3-Dicyclohexylcarbodiimide; THF = Tetrahydrofuran
Part No.
7.8 x 10 mm Cartridge Holder

186000708
289000779
186000709

700001019
700001436
700001020
www.waters.com
Atlantis Prep OBD Columns
Bridging the Performance Gap for Polar Compound Retention
Beta Blockers
Atlantis T3
4.6 x 100 mm, 5 m
3.0
2
AU
2.0
Flow Rate:
Inj. Vol.:
V 0 = 1.5 min
1.0
1.0 mL/min
15 L (6.6 mg total mass load)
0.0
Atlantis Prep T3 OBD

19 x 100 mm, 5 m
3
3.0
2
AU
2.0
1.0
Flow Rate:
Inj. Vol.:
V 0 = 1.5 min
17.06 mL/min
0.0
3
Atlantis Prep T3 OBD

30 x 100 mm, 5 m
3.0
2
AU
2.0
First introduced in 2002, the Atlantis product range has rapidly gained
acceptance as the industry standard for polar compound retention and
purification. Available for both reversed-phase and HILIC modes of
c hromatography, these phases exhibit significantly increase retention
c haracteristics as compared to conventional C18 or embedded polar
group column c hemistries.
Atlantis dC18
T he first in the family to be introduced, Atlantis dC18 is a reversedphase packing bonded with a difunctional C18 ligand to an intermediate
ligand density (1.6 moles/m2) and fully end-capped. For maximum
column lifetime, the dC18 phase is recommended for mobile-phase pH
values of 2.56.
Atlantis T3
An evolution of the dC18 product, Atlantis T3 columns retain all of the
benefits of its predecessor but exhibit significantly improved stability
in low-pH mobile phases as well as improved peak shape for amine
containing bases at pH 7. T3 bonding utilizes a trifunctional C18 ligand,
(1.6 moles/m2), and a proprietary end-capping process whic h has been
proven to be muc h more effective than the traditional trimethyl silane
(TMS) end-capping.
V 0 = 1.5
1.0
min
Flow Rate:
Inj. Vol.:
42.53 mL/min
0.0
0
Analytical Gradient Table

Gradient:
Time
(min)
0.0
1.0
2.0
6.0
8.0
8.1
10.0
10.1
15.0
Profile
%A
100
100
82
28
28
0
0
100
100
Preparative Gradient Table

Gradient:
Time
(min)
0.0
2.0
3.0
7.0
9.0
9.1
11.0
11.1
16.0
Profile
%A
100
100
82
28
28
0
0
100
100
%B
0
0
18
72
72
100
100
0
0
10
12 Min.
Compounds
1. Atenolol
2. Metoprolol
3. Propranolol
40 mg/mL in DMSO
200 mg/mL in DMSO
200 mg/mL in DMSO
Mobile Phase A:
Mobile Phase B:
Detection:

UV @ 280 nm
Instrument:
2525 Binary Gradient Module,

2767 Sample Manager, 2996
PDA (AutoPurification flow cell)
Ambient
Temperature:
%B
0
0
18
72
72
100
100
0
0
Literature
References
www.waters.com
Atlantis T3 and ACQUITY UPLC

HSS T3 Columns Brochure,



187
Nucleic Acid Bases Scale-up on Atlantis HILIC Columns
HILIC is a variation of normal-phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of
organic (non-polar) solvent and a low concentration of aqueous (polar)
solvent. In HILIC, the organic portion of the mobile phase is the weak
solvent, the aqueous portion the strong solvent and the compounds elute in
the order of increasing hydrophilicity. Due to the high organic, low viscosity
mobile phases that are employed, HILIC provides low column backpressure. Additionally, the collected fractions of interest are inherently high in
organic, enabling faster solvent evaporation compared to reversed phase.
Columns:
Mobile Phase A:
Mobile Phase B:
Gradients:
Flow Rates:
Injection Volumes:
Weak Wash Solvent:
Temperature:
Detection:
Instrument:
Atlantis HILIC, 4.6 x 50 mm, 5 m (SN: 010536275106 02)

Atlantis HILIC OBD Prep, 19 x 50 mm, 5 m (SN: 01061631910F 01)
10 mM NH4COOH, pH 3 buffer
Acetonitrile/100 mM NH4COOH, pH 3 (90/10) v/v
As indicated on chromatograms
1.4 mL/min (analytical); 23.9 mL/min (preparative)
25 g/mL each in ACN/MeOH (75/25) v/v
60 L (analytical); 1020 L (preparative)
95/5 ACN/H2O
Ambient
UV @ 280 nm
Waters AutoPurification System with 996 PDA
Void is at 0.56 min preparative, 0.55 min analytical

Atlantis HILIC,
4.6 x 50 mm, 5 m
Gradient:
Time
Profile
(min)
%A
%B
0.0
0
100
5.0
10
90
6.0
10
90
6.1
0
100
10.0
0
100
Total Loading:
4500 ng
2
3
Atlantis Prep HILIC OBD,

19 x 50 mm, 5 m
Gradient:
Time
Profile
(min)
%A
%B
0.0
0
100
0.74
0
100
5.74
10
90
6.74
10
90
6.84
0
100
10.74 0
100
Total Loading:
76.5 g
2
3
1
Atlantis Prep Columns
Type
Particle
Size
T3
10 x 10 mm
10 x 50 mm
10 x 100 mm
10 x 150 mm
10 x 250 mm
19 x 10 mm
OBD 19 x 50 mm
OBD 19 x 100 mm
OBD 19 x 150 mm
OBD 19 x 250 mm
OBD 30 x 50 mm
OBD 30 x 75 mm
OBD 30 x 100 mm
OBD 30 x 150 mm
OBD 50 x 50 mm
OBD 50 x 100 mm
OBD 50 x 150 mm
Guard
Column
Column
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
10 x 10 mm
10 x 150 mm
10 x 250 mm
19 x 10 mm
OBD 19 x 50 mm
OBD 19 x 150 mm
OBD 19 x 250 mm
OBD 30 x 75 mm
OBD 30 x 150 mm
OBD 30 x 250 mm
OBD 50 x 50 mm
OBD 50 x 100 mm
OBD 50 x 150 mm
OBD 50 x 250 mm
Guard
Column
Column
Guard
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
Dimension
188
HILIC
dC18
186003695
186003691
186003692
186003693
186003694
1860036992
186003696
186003697
186003698
186004026
186003700
186003701
186003702
186003703
186004080
186004081
186004082
1860039562
186003957
186003958
186003959
186003960
186003961
186003962
1860023001
186002298
186002299
1860013612
186001365
186001367
186002800
186004030
186001373
186002455
186001375
186002801
1860037061
186003704
186003705
1860037102
186003707
186003708
186003709
186004712
186003711
186003712
186004083
186004084
186004085
186004086
1860024521
186002453
186002454
1860013632
186001369
186001371
186002417
186002418
Compounds
1. Uracil
2. 5-Fluorocytosine
3. Cytosine
4 Min.

Description
1
2
Part No.
289000779
186000709

700001019
700001436
700001020

www.waters.com
XTerra Prep OBD Columns
XTerra Prep Columns

Type
Particle
Size
MS C18
MS C8
RP18
RP8
7.8 x 10 mm
7.8 x 50 mm
7.8 x 100 mm
7.8 x 150 mm
10 x 10 mm
10 x 30 mm
10 x 50 mm
10 x 100 mm
10 x 150 mm
19 x 10 mm
OBD 19 x 50 mm
OBD 19 x 100 mm
OBD 19 x 150 mm
OBD 30 x 50 mm
OBD 30 x 75 mm
OBD 30 x 100 mm
OBD 50 x 50 mm
OBD 50 x 100 mm
Cartridge
Column
Column
Column
Cartridge
Column
Column
Column
Column
Cartridge
Column
Column
Column
Column
Column
Column
Column
Column
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
1860011686
186001152
186001156
186001475
1860010017
186001010
186001014
186001018
186001479
1860011048
186001930
186001934
186002379
186001938
186002387
186001942
186002218
186002222
1860011696
186001153
186001157
186001476
1860010047
186001011
186001015
186001019
186001480
1860011058
186001931
186001935
186002380
186001939
186002388
186001943
186002219
186002223
1860011706
186001154
186001158
186001477
1860010067
186001012
186001016
186001020
186001481
1860011068
186001932
186001936
186002381
186001940
186002389
186001944
186002220
186002224
1860011716
186001155
186001159
186001478
1860010087
186001013
186001017
186001021
186001482
1860011078
186001933
186001937
186002382
186001941
186002390
186001945
186002221
186002225
7.8 x 10 mm
7.8 x 150 mm
7.8 x 300 mm
10 x 10 mm
10 x 150 mm
10 x 250 mm
10 x 300 mm
19 x 10 mm
OBD 19 x 50 mm
OBD 19 x 150 mm
OBD 19 x 250 mm
OBD 19 x 300 mm
OBD 30 x 150 mm
OBD 30 x 250 mm
OBD 30 x 300 mm
OBD 50 x 50 mm
OBD 50 x 150 mm
OBD 50 x 250 mm
Cartridge
Column
Column
Cartridge
Column
Column
Column
Cartridge
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
1860011726
186001160
186001164
1860010027
186001022
186001026
186001030
1860010348
186002254
186002255
186002259
186002263
186002267
186002271
186002275
186002279
186002843
186002847
1860011736
186001161
186001165
1860010057
186001023
186001027
186001031
1860010358
186002256
186002260
186002264
186002268
186002272
186002276
186002280
186002844
186002848
1860011746
186001162
186001166
1860010077
186001024
186001028
186001032
1860010368
186002257
186002261
186002265
186002269
186002273
186002277
186002281
186002845
186002849
1860011756
186001163
186001167
1860010097
186001025
186001029
186001033
1860010378
186002258
186002262
186002266
186002270
186002274
186002278
186002282
186002846
186002850
Dimension
XTerra Chemistries
XTerra MS
XTerra MS C18 and MS C8 columns were designed to be
compatible with Mass Spectrometry applications and
provide sharp peaks, good sensitivity, and large peak
capacities. T hese chemistries exhibit peak shape usually
only ac hievable with columns employing embedded
polar group tec hnology (see XTerra RP columns). T he
tri-functional bonding chemistries of XTerra MS columns
deliver the longest lifetimes over the widest pH range
(pH 1-12) and provide maximum throughput with
excellent resolution and ultra-low bleed.
XTerra RP
XTerra R P18 and R P8 columns combine Hybrid
Particle Tec hnology wit h S hield Tec hnology by
incorporating an embedded polar group to deliver
the best possible peak shape. T hese general purpose
columns allow fast method development by offering unique selectivity. Like all XTerra columns, the
shielded XTerra RP columns exhibit excellent water
wettability, even in 100% aqueous mobile phases.
T he combination of a greatly widened pH range
for selectivity choices and the additional selectivity
options of two ligands are often the only tools
necessary for successful method development.
www.waters.com
Requires 7.8 x 10 mm Cartridge Holder, Part No. 186000708

8
6
7

Description
Part No.
289000779
186000709

700001019
700001436
700001020
189
Symmetry Columns
SymmetryPrep Columns for Purification and Isolation
Dimension
Symmetry columns provide the highest standard of reproducibility for total

confidence in the long-term compliance of your HPLC methods. Symmetry
columns demonstrate excellent peak symmetry for maximum sensitivity and
accurate quantitation. Use SymmetryPrep columns for lead generation and
optimization for development of validated assays for stability testing and
impurity profiles.
C18
C8
7.8 x 10 mm
7.8 x 50 mm
7.8 x 100 mm
19 x 10 mm
19 x 50 mm
19 x 100 mm
30 x 50 mm
30 x 100 mm
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186000711
186000208
186000209
1860007151
186000210
186000211
186000235
186000236
186000712
186000214
186000215
1860007161
186000216
186000229
186000237
186000238
7.8 x 10 mm
7.8 x 150 mm
7.8 x 300 mm
19 x 10 mm
19 x 150 mm
19 x 300 mm
7 m
7 m
7 m
7 m
7 m
7 m
186000713
WAT066288
WAT066235
1860007171
WAT066240
WAT066245
186000714
WAT066285
WAT066225
1860007181
WAT066228
WAT066230
SymmetryShield Columns for Purification and Isolation

Dimension
SymmetryPrep Columns
SymmetryPrep 7 m columns are manufactured for t he c hemist w ho
needs to purify new chemical entities or isolate impurities or degradants.
T he SymmetryPrep 7 m products are a family of high-capacity, highefficiency, high-performance liquid chromatography (HPLC) columns that
have identical selectivity to t he Symmetry 3.5 and 5 m particle
size analytical columns. T his guarantees direct method scale-up with no
further development. SymmetryPrep columns deliver the advantages of
unmatched peak shape and resolution using simple volatile mobile-phase
buffers such as TFA and ammonium acetate. All of these features result in a
tremendous time reduction in your research and development programs.
Particle Size
Particle Size
RP18
19 x 10 mm
19 x 50 mm
19 x 100 mm
19 x 150 mm
5 m
5 m
5 m
5 m
1860018351
186001836
186001837
186001838
1860018411
186001842
186001843
186001844
RP8
19 x 150 mm
19 x 300 mm
7 m
7 m
186001839
186001840
186001845
186001846
Symmetry300 Columns for Purification and Isolation

Dimension
19 x 10 mm
19 x 50 mm
19 x 100 mm
19 x 150 mm
Particle Size
5 m
5 m
5 m
5 m
C18
1860018471
186001848
186001849
186001850
Symmetry300 is also available as a C4 chemistry and in a 7 m particle size. Please inquire.

1
Requires 19 x 10 mm Prep Guard Cartridge Holder, Part No. 186000709
SymmetryShield columns with Shield Tec hnology contain a patented

chemistry for reversed-phase HPLC that delivers significant changes in
selectivity for polar and basic compounds. SymmetryShield columns
deliver unique reversed-phase selectivity with significantly improved
peak shape and resolution even for the most c hallenging analytes.
Description
Symmetry300 Columns
Symmetry300, reversed-phase columns are designed specifically for high
recoveries of peptides and proteins. Symmetry300 columns are the best
solution for validation compliance regarding identity, purity, and stability.
190
Part No.
7.8 x 10 mm Cartridge Holder

186000708
289000779
186000709

700001019
700001436
700001020
www.waters.com
Waters Spherisorb Columns
Waters Spherisorb columns are the most widely referenced HPLC columns
in scientific literature. T here are over 2,000 abstracts published on
Spherisorb columns, providing a tremendous range of validated methods
and applications to assist you in your method development process.
Waters Spherisorb 10 m Semiprep Columns

Chemistry
10 x 250 mm
20 x 250 mm
S10 ODS2
S10 ODS1
S10 C8
S10 C6
S10 C1
S10 NH2
S10 P
S10 CN
S10 W
PSS832585
PSS830785
PSS832885
PSS833285
PSS833085
PSS833685
PSS833885
PSS833585
PSS830285
PSS832595
PSS830795
PSS832895
PSS833295
PSS833095
PSS833695
PSS833895
PSS833595
PSS830295
PSS833985
PSS837685
PSS833995
PSS837695
Dimension
Part No.
3.9 x 300 mm
7.8 x 300 mm
19 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
19 x 300 mm
WAT038500
WAT025820
WAT025822
WAT038501
WAT025821
WAT025823
Dimension
Part No.
3.9 x 300 mm
7.8 x 300 mm
19 x 150 mm
19 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
19 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
19 x 150 mm
19 x 300 mm
3.9 x 300 mm
19 x 300 mm
WAT027324
WAT084176
WAT088500
WAT025828
WAT025875
WAT025832
WAT025834
WAT027198
WAT084179
WAT084177
WAT084040
WAT084178
WAT027477
WAT084175
WAT091648
WAT025829
WAT025874
WAT025835
Ion Exchange
S10 SAX
S10 SCX
Prep Nova-Pak HR Columns

Prep Nova-Pak HR 6 m, ultra high-efficiency packing materials can be used
in shorter columns to obtain the same resolution as longer columns with
larger particle size packing materials. S horter columns mean faster
separation, lower solvent consumption and more concentrated fractions.
Prep Nova-Pak HR has the same selectivity and retention c haracteristics
as the analytical 4 m Nova-Pak material. T he Prep Nova-Pak HR packing
materials are ideal for the separation of a wide range of compounds such
as organic synthesis intermediates or natural products.
Prep Nova-Pak HR Columns
Description
Particle
Size
Pore
Size
6 m
60
Prep Nova-Pak HR Silica
6 m
60
Bondapak/Porasil Columns
T he popular Bondapak C18 chemistry and Porasil silica packing materials
are available in three different particle sizes, 10, 15-20, and 37-55 m,
providing easy transfer of c hromatography met hodology from one
particle size to another. You can utilize your existing 10 m Bondapak
or Porasil c hromatography as a starting point for scale-up or, if you are
developing a new purification protocol, investigate whic h preparative
particle size provides the best balance between resolution, throughput
and cost.
T he preparative Bondapak HC18 HA (high-carbon load, high-activity silica) is
a highly carbon-loaded packing that provides different selectivity over the
standard Bondapak packing materials. T he higher carbon load on the silica
surface typically results in a higher loading capability. Bondapak HC18 HA
is available in a 37-55 m particle size.
T he Prep family of packing materials is based on a 55-105 m Porasil
silica and provides a cost effective means for scale-up of preparative
processes. T he selectivity of Prep silica is identical to 10, 15-20, and
37-55 m Porasil while Prep C18 has a slightly different selectivity than
Bondapak packings.
www.waters.com
Bondapak/ Porasil Columns
Description
Particle
Size
Pore
Size
Bondapak C18
10 m
125
Bondapak C18
15-20 m
125
Bondapak Phenyl
10 m
125
Bondapak CN
Bondapak NH2
10 m
10 m
125
125
Porasil
10 m
125
Porasil
15-20 m
125
191
Delta-Pak Packing
Delta-Pak packing materials are ideal for the separation of peptides,
proteins, and natural products. T he isolation and purification of a peptide
is usually a multi-step procedure with the fractions from the first run
being re-chromatographed on the same preparative column to obtain pure
product. Delta-Pak packing materials are based on a highly stable, bonded,
endcapped 5 and 15 m packing. T he 5 m packing is available in analytical scale dimensions for preliminary preparative chromatographic studies,
peptide mapping, and fraction purity assays. T he chemistry characteristics
of the packing materials are independent of the particle size.
Delta-Pak Columns
Description
Particle
Size
Pore
Size
Dimension
Part No.
Delta-Pak C18
15 m
Delta-Pak C4
15 m
100
300
100
300
100
300
100
300
100
100
300
100
300
100
300
100
300
3.9 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
19 x 300 mm
19 x 300 mm
30 x 300 mm
30 x 300 mm
50 x 300 mm
3.9 x 300 mm
3.9 x 300 mm
7.8 x 300 mm
7.8 x 300 mm
19 x 300 mm
19 x 300 mm
30 x 300 mm
30 x 300 mm
WAT011797
WAT011802
WAT011798
WAT011803
WAT011799
WAT011804
WAT011800
WAT011805
WAT011801
WAT011807
WAT011812
WAT011808
WAT011813
WAT011809
WAT011814
WAT011810
WAT011815
Preparative Bulk Material

Waters offers a number of different bulk packing materials for
lab-to-process-scale purifications. All our packing materials are
manufactured under cGMP (current Good Manufacturing Practices)
ensuring a reproducible material for long-term reproducibility. Our
manufacturing facilities are ISO 9002-certified and are registered with
the FDA as a medical device manufacturing facility. Bulk materials are available packaged in quantities of 100 g up to 5 kg. For larger
quantity purchases, please inquire about pricing and availability.
Normal Phase Bulk Packings
Reversed-Phase Bulk Packings
Particle
Size
Pore
Size
Porasil Silica
15-20 m
125
Porasil Silica
37-55 m
125
Prep Silica
55-105 m
125
Description
192
All bulk packings are carefully quality

controlled using a wide array of physical
and chemical tests to ensure consistent
batch-to-batch reproducibility.
Dimension
Part No.
100 g
1 kg
5 kg
100 g
1 kg
5 kg
100 g
1 kg
5 kg
WAT020731
WAT020732
WAT020733
WAT020721
WAT020722
WAT020723
WAT020587
WAT010004
WAT020588
Particle
Size
Pore
Size
Bondapak C18
15-20 m
125
Bondapak C18
37-55 m
125
Bondapak HC18 HA
37-55 m
125
Prep C18
55-105 m
125
Description
Dimension
Part No.
100 g
1 kg
5 kg
100 g
1 kg
5 kg
100 g
1 kg
5 kg
100 g
1 kg
5 kg
WAT020739
WAT020740
WAT020741
WAT030632
WAT030633
WAT030634
WAT035672
WAT035674
WAT035676
WAT020594
WAT010001
WAT020595
www.waters.com
Radial Compression Modules
We carry a complete inventory of accessories and spare parts for the Waters
patented Radial Compression Modules for use with the 5 mm and 8 mm i.d.
Radial-Pak column segments, the 25 mm and 40 mm i.d. PrepLC column
segments, and the 47 mm and 57 mm i.d. PrepPak cartridges.
Nova-Pak and Prep Nova-Pak Radial Compression

Column Segments and PrepPak Cartridges
Column
Particle Size
Pore Size
Dimension
Part No.
5 x 100 mm
8 x 100 mm
5 x 100 mm
8 x 100 mm
5 x 100 mm
8 x 100 mm
5 x 100 mm
8 x 100 mm
5 x 100 mm
8 x 100 mm
WAT080100
WAT086342
WAT035890
WAT035884
WAT010657
WAT010658
WAT010224
WAT010223
WAT010986
WAT010987
8 x 100 mm
8 x 100 mm
WAT025843
WAT025844
Nova-Pak Radial-Pak Column Segments
8 x10 Holder
8 x 10 Holder for 8 x 100 mm and
5 x 100 mm Radial-Pak column segments
Description
Part No.
8 x 10 Holder
8 x 10 Extension Kit (includes 1 extension tube, union, o-rings)
Cartridge Union
WAT082887
WAT038846
WAT038849
Nova-Pak C18
4 m
60
Nova-Pak C8
4 m
60
Nova-Pak Phenyl
4 m
60
Nova-Pak CN HP
4 m
60
Nova-Pak Silica
4 m
60
Prep Nova-Pak HR Radial-Pak Column Segments
PrepLC 25 mm Module

PrepLC 25 mm Module for

25 x 100 mm column segments
6 m
6 m
60
60
Prep Nova-Pak HR PrepLC 25 mm Column Segments
Description
Part No.
PrepLC 25 mm Module
PrepLC 25 mm Extension Kit (includes 1 extension tube, union, o-rings)
Extension Tube
WAT015814
WAT022180
WAT019311

6 m
6 m
60
60
25 x 100 mm WAT038510
25 x 100 mm WAT038511
Prep Nova-Pak HR 25 x 10 Guard-Pak Inserts (2/pk)

6 m
6 m
60
60
25 x 10 mm
25 x 10 mm
WAT038528
WAT038530
Prep Nova-Pak HR PrepLC 40 mm Column Segments
PrepLC Assemblies
PrepLC Assembly for
40 x 100 mm and
25 x 100 mm
column segments

PrepLC
Assembly*
40 x 100 mm

* Note: Up to two extension tubes can be attached

for a combined cartridge length of 300 mm
www.waters.com
40 x 100 mm WAT037704
40 x 100 mm WAT037708
6 m
6 m
60
60
40 x 10 mm
40 x 10 mm
WAT037854
WAT037857
Resolve Radial Compression Column Segments

and PrepPak Cartridges*
Column
PrepLC 40 mm Assembly
(includes PrepLC Universal Base and PrepLC 40 mm Chamber)
PrepLC Universal Base
PrepLC 40 mm Chamber (includes o-rings, spacer and union)
PrepLC 40 mm Extension Kit (includes extension tube, union, o-rings)
PrepLC 25 mm Chamber (includes o-rings, spacer and union)
PrepLC Scale-up Kit with capability for 40 mm or 25 x 300 length
includes: 1 - PrepLC Universal Base
2 - PrepLC Chambers (one each of 40 mm and 25 mm)
2 - PrepLC 25 mm Extension Kits
2 - PrepLC 40 mm Extension Kits
60
60
Prep Nova-Pak HR 40 x 10 Guard-Pak Inserts (2/pk)
PrepLC
Assembly*
25 x 100 mm
Description
6 m
6 m
Part No.
WAT022441
WAT027577
WAT027578
WAT022365
WAT033994
WAT022180
WAT022440
Particle Size
Pore Size
Dimension
Part No.
Resolve C18
Resolve C18
5 m
10 m
90
90
Resolve C8
10 m
90
Resolve CN
10 m
90
Resolve Silica
Resolve Silica
5 m
10 m
90
90
8 x 100 mm
5 x 100 mm
8 x 100 mm
5 x 100 mm
8 x 100 mm
5 x 100 mm
8 x 100 mm
8 x 100 mm
5 x 100 mm
8 x 100 mm
WAT084624
WAT084620
WAT084720
WAT085672
WAT085670
WAT084626
WAT084636
WAT084634
WAT084630
WAT084730
* All column segments and cartridges require the

appropriate holder/module, see page 195
193
Bondapak/ Porasil Radial Compression Column

Segments and PrepPak Cartridges*
Column
Particle Size
Pore Size
Delta-Pak Radial Compression Column Segments

and PrepPak Cartridges*
Dimension
Part No.
Bondapak/Porasil Radial-Pak Column Segments

Bondapak C18
Bondapak NH2
Bondapak Phenyl
Porasil
Bondapak C18
Bondapak HC18 HA
10 m
10 m
10 m
10 m
15-20 m
37-55 m
10 m
15-20 m
Bondapak HC18 HA
Porasil
Porasil
37-55 m
10 m
15-20 m
Particle Size
125
125
125
125
125
125
8 x 100 mm
8 x 100 mm
8 x 100 mm
8 x 100 mm
8 x 100 mm
8 x 100 mm
WAT085721
WAT085724
WAT085722
WAT085720
WAT025841
WAT036561
125
125
300
125
125
125
25 x 100 mm
25 x 100 mm
25 x 100 mm
25 x 100 mm
25 x 100 mm
25 x 100 mm
WAT038505
WAT038503
WAT038575
WAT056970
WAT038504
WAT038502
25 x 10 mm
25 x 10 mm
25 x 10 mm
25 x 10 mm
WAT038518
WAT038514
WAT038516
WAT038512
40 x 100 mm
40 x 100 mm
40 x 100 mm
40 x 100 mm
40 x 100 mm
WAT037684
WAT037676
WAT037712
WAT037680
WAT037672
40 x 10 mm
40 x 10 mm
40 x 10 mm
40 x 10 mm
WAT037839
WAT037833
WAT037836
WAT037830
Delta-Pak C18
15 m
Delta-Pak C4
15 m
10 m
15-20 m
10 m
15-20 m
Delta-Pak C18
15 m
Delta-Pak C4
15 m
10 m
15-20 m
Porasil
Porasil
10 m
15-20 m
Part No.
100
300
100
8 x 100 mm
8 x 100 mm
8 x 100 mm
WAT025846
WAT025845
WAT025848
100
300
100
300
25 x 100 mm
25 x 100 mm
25 x 100 mm
25 x 100 mm
WAT038506
WAT038507
WAT038508
WAT038509
100
300
100
300
25 x 10 mm
25 x 10 mm
25 x 10 mm
25 x 10 mm
WAT038520
WAT038522
WAT038524
WAT038526
100
300
100
300
40 x 100 mm
40 x 100 mm
40 x 100 mm
40 x 100 mm
WAT037688
WAT037692
WAT037696
WAT037700
40 x 10 mm
40 x 10 mm
40 x 10 mm
WAT037842
WAT037845
WAT037851
Delta-Pak Prep 25 x 10 Guard-Pak Inserts (2/pk)

Delta-Pak C18
15 m
Delta-Pak C4
15 m
125
125
125
125
Delta-Pak PrepLC 40 mm Column Segments

Delta-Pak C18
15 m
Delta-Pak C4
15 m
Bondapak/Porasil PrepLC 40 mm Column Segments

Bondapak C18
Bondapak C18
Dimension
Delta-Pak PrepLC 25 mm Column Segments
Bondapak/Porasil Prep 25 x 10 Guard-Pak Inserts (2/pk)

Bondapak C18
Bondapak C18
Porasil
Porasil
Pore Size
Delta-Pak Radial-Pak Column Segments
Bondapak/Porasil PrepLC 25 mm Column Segments

Bondapak C18
Bondapak C18
Column
125
125
300
125
125
Delta-Pak Prep 40 x 10 Guard-Pak Inserts (2/pk)

Delta-Pak C18
15 m
Delta-Pak C4
15 m
100
300
300
Bondapak/Porasil Prep 40 x 10 Guard-Pak Inserts (2/pk)

Bondapak C18
Bondapak C18
Porasil
Porasil
10 m
15-20 m
10 m
15-20 m
125
125
125
125
PrepPak Cartridges
Requires PrepPak 1000 Module
(Part No. WAT089592)
Column
Particle Size
Pore Size
Dimension
Part No.
Bondapak C18
Bondapak C18
Bondapak C18
Bondapak HC18 HA
Bondapak NH2
Delta-Pak C18
15-20 m
15-20 m
37-55 m
37-55 m
55-105 m
15 m
125
300
125
125
125
100
300
100
300
125
125
47 x 300 mm
47 x 300 mm
47 x 300 mm
47 x 300 mm
47 x 300 mm
47 x 300 mm
47 x 300 mm
47 x 300 mm
47 x 300 mm
47 x 300 mm
47 x 300 mm
WAT091784
WAT038571
WAT025873
WAT038570
WAT091631
WAT015401
WAT010988
WAT011633
WAT011669
WAT025876
WAT025852
WAT025878
WAT025853
WAT025877
WAT089592
Delta-Pak C4
15 m
Prep C18
55-105 m
Porasil Silica (single)
15-20 m
(10/case)
Porasil Silica (single)
37-55 m
125
47 x 300 mm
(10/case)
PrepPak 1000 Module for 47 x 300 mm PrepPak Cartridges
* All column segments and cartridges require the

appropriate holder/module, see page 195
194
www.waters.com
Accessories and Spare Parts for Waters Radial
Compression Cartridge Holders and Modules
We carry a complete inventory of accessories and spare parts for
Waters patented Radial Compression Modules for use with the 5 mm
and 8 mm i.d. x 100 mm long Radial-Pak column segments, 25 mm
and 40 mm i.d. x 100 mm long PrepLC column segments, and 47 mm or
57 mm i.d. x 300 mm long PrepPak cartridges.
8 x10 Cartridge Holder, Parts and Accessories

Description
8 x 10 Holder
8 x 10 Extension Kit (includes 1 extension tube, union, o-rings)
Column segment union
O-ring for extension tube
Connector tubing assembly (non-metallic)
Connector assembly (stainless steel)
Washer for connectors, 10/pk
Pressure relief plug
Check valve
O-ring (large) for connector, 10/pk
O-ring (small) for connector (normal phase), 4/pk
O-ring (small) for connector (reversed-phase), 10/pk
O-ring for filling port, 10/pk
O-ring for pressure piston
Gripper ring replacement kit
(includes 10 gripper rings, 20 washers, 10 ferrules and tool)
PrepLC Spare Parts

Part No.
WAT082887
WAT038846
WAT038849
WAT038851
WAT088919
WAT082892
WAT005147
WAT088027
WAT082888
WAT005130
WAT015797
WAT005129
WAT005129
WAT088494
WAT021908
PrepLC 25 mm Module, Parts and Accessories

Description
PrepLC 25 mm Module
Extension tube
O-ring for extension tube
O-ring (large) for connector
O-ring (small) for connector (normal phase)
O-ring (small) for connector (reversed-phase)
O-ring for filling port, 10/pk
Union coupling assembly
Union, 1/8 to 1/16 inch tubing, 5/pk
Part No.
WAT015814
WAT022180
WAT019311
WAT015831
WAT015833
WAT015848
WAT015834
WAT005129
WAT015854
WAT015860
WAT005137
Description
Part No.
PrepLC Universal Base Spare Parts

O-ring removal tool
O-ring for filling port
Filling port plug
Ferrules and compression fittings (stainless steel) 5/pk
WAT082853
WAT022281
WAT005129
WAT027509
WAT025604
PrepLC 40 mm Chamber Spare Parts

Cartridge spacer
O-ring, base plate (small)
O-ring, base plate (large)
O-ring, chamber top
O-ring (normal phase) cartridge top and bottom, spacers and unions
O-ring (normal phase) chamber bottom
O-ring (normal phase) inner connector, top and bottom
O-ring (reversed-phase) cartridge top and bottom, spacers and unions
O-ring (reversed-phase) chamber bottom
O-ring (reversed-phase) inner connector, top and bottom
O-ring, extension tube
WAT033996
WAT033997
WAT022453
WAT022454
WAT022280
WAT027519
WAT022299
WAT022297
WAT027518
WAT022283
WAT015835
WAT022454
PrepLC 25 mm Chamber Spare Parts

Segment spacer
O-ring, base plate (small)
O-ring, base plate (large)
O-ring, chamber top
O-ring, (normal phase) cartridge top and bottom, spacers and unions
O-ring (normal phase) chamber bottom
O-ring (normal phase) inner connector, top and bottom
O-ring (reversed-phase) cartridge, top and bottom, spacers and union
O-ring (reversed-phase) chamber bottom
O-ring (reversed-phase) inner connector, top and bottom
Tubing Fluid Path Kit* (PEEK)
(includes inner connectors, tubing, ferrules and compression screws)
WAT015860
WAT015859
WAT022276
WAT015831
WAT015833
WAT015848
WAT022298
WAT022297
WAT015834
WAT022282
WAT015835
WAT022400
* For applications where a metal-free flow path is needed.
www.waters.com
195
VIRIdIS SFC COLUMNS
TM
Viridis SFC Columns

Combining state-of-the-art media manufacturing with the industry
leading column technology, Viridis Supercritical Fluid Chromatography
(SFC) columns bring a new level of reproducibility to the world of
laboratory-scale purification.
Viridis SFC columns are now available for analytical and preparative
c hromatography as:
Viridis SFC 2-Ethylpyridine columns
Viridis SFC Silica columns
Viridis SFC 2-Ethylpyridine Columns

2-Ethylpyridine chemistry is successfully used for a wide range of achiral
SFC applications due to its retention, peak shape and selectivity properties.
Viridis SFC 2-Ethylpyridine columns also provide high reliability and
reproducibility and can be used for analytical and preparative applications.
Si
2-Ethylpyridine Ligand
Viridis SFC columns, manufactured

for reliability and consistency
As a primary manufacturer of silica and hybrid chromatographic particles,

all stages of product synthesis are meticulously monitored and controlled,
ensuring complete characterization of the finished product.
T his level of manufacturing process understanding delivers chromatographic
media with guaranteed consistent performance year after year.
OBD Column Design

Improved Mechanical Stability
Laboratory-scale SFC purification presents many
challenges to the chromatographer. Inconsistency in
column-to-column performance and lifetimes often
result in lost samples, repeat purification runs and
poor scalability from small to large volume columns. Common SFC prep
column failures are due to mec hanical instabilities, especially voids
in t he packed bed. T he Viridis SFC prep OBD columns combine t he
patented optimized packing process with a unique set of column designs
ensuring increased lifetime and greater reproducibility.
196
Viridis SFC Columns
Excellent Peak Shape

Viridis SFC 2-Ethylpyridine columns ex hibit good peak shape whether
separations are performed with or without additives suc h as diethylamine or ammonium acetate.
Column:
Part Number:
Gradient Separation:
Flow Rate:
Wavelength:
System Back Pressure:
Injection Volume:
Viridis SFC 2-Ethylpyridine Column,

4.6 x 150 mm, 5 m
186004937
10 min
2.5 mL/min at 40 C
254 nm
100 bar
3 L
Compounds:
1. Caffeine
2. Amitriptyline
3. Thymine
4. Cytosine
5. Sulfamethoxazole
6. Sulfamethizole
300
250
Absorbance (mAU)
Based upon over 50 years of experience in the separation science industry,

Waters chromatographic media sets the standard for quality and batch-tobatch reproducibility.
200
150
100
50
0
-50
0
Mobile Phase:
5-40% methanol with
no additive
Mobile Phase:
5-40% methanol with
0.2% diethylamine (DEA)
10 min
Mobile Phase:
5-40% methanol with
20 mM ammonium acetate
www.waters.com
VIRIdIS SFC COLUMNS

High Mass Loading
ORDERING INFORMATION
Viridis SFC 2-Ethylpyridine columns provide excellent mass loading to

ensure maximum throughput.
Column:
Viridis SFC 2-Ethylpyridine OBD Column,

19 x 150 mm, 5 m
186004945
4.2 min
5-15% methanol
with no additive
85 g/min at 40 C
Part Number:
Mobile Phase:
Flow Rate:
Wavelength:
System Backpressure:
Compounds:
80 mg/mL
220 nm
120 bar
1. Coumarin
2. Flavone
3. Ibuprofen
4. Carbamazepine



0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
2.25
2.50
2.75
3.00 min
Viridis SFC Silica Columns
Chemistry
2-Ethylpyridine
Silica
Format
Column
Column
Column
Column
Column
Column
Column
Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
Particle
Shape
Spherical
Spherical
Dimension
4.6 x 50 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
10 x 50 mm
10 x 100 mm
10 x 150 mm
10 x 250 mm
19 x 50 mm
19 x 100 mm
19 x 150 mm
19 x 250 mm
30 x 50 mm
30 x 75 mm
30 x 100 mm
30 x 150 mm
30 x 250 mm
50 x 50 mm
50 x 100 mm
50 x 150 mm
50 x 250 mm
Pore Size
()
100
100
Surface
Area (m2/g)
340
340
Pore Vol.
(cc/g)
0.90
0.90
Carbon
Load (%)
8
n/a
Endcapped
no
n/a
Particle
Size
Viridis SFC
2-Ethylpyridine
Viridis SFC
Silica
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186004935
186004936
186004937
186004938
186004939
186004940
186004941
186004942
186004943
186004944
186004945
186004946
186004947
186004948
186004949
186004950
186004951
186004952
186004953
186004954
186004955
186004908
186004909
186004910
186004911
186004912
186004913
186004914
186004915
186004916
186004917
186004918
186004919
186004920
186004921
186004922
186004923
186004924
186004925
186004926
186004927
186004928
Based on Waters long history of chromatographic silica production, the Viridis

SFC Silica columns are designed to be highly reproducible and predictable
based on tight product specifications and very low metal content.
Selectivity
Viridis SFC Silica columns offer added selectivity due to the highly polar,
unbonded surface and will have greater interaction with polar functional
groups when compared to Viridis SFC 2-Ethylpyridine columns.
Viridis SFC Silica Column vs. Viridis SFC 2-Ethylpyridine Column
Conditions
Mobile Phase:
Flow Rate:
Wavelength:
System Backpressure:
Compounds:
1. Flavone
2. Ibuprofen
3. Carbamazepine
4.2 min
5-15% methanol with no additive
85 g/min at 40 C
220 nm
120 bar
1
6.0e-1
Viridis SFC Silica OBD Column,

19 x 150 mm, 5 m
4.0e-1
2.0e-1
Literature
References
Viridis SFC Columns Brochure,
Basic Principles for Large-Scale Supercritical
Fluid Chromatography Application Note,
0.0
6.0e-1
2
3
4.0e-1
Viridis SFC 2-Ethylpyridine OBD Column,

19 x 150 mm, 5 m
2.0e-1
0.0
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50 min
Using the same SFC gradient conditions, different selectivity can be achieved by using a Viridis SFC 2-Ethylpyridine
column or Viridis SFC silica column. The retention time for ibuprofen was reduced and the carbamazepine retention
time was increased by switching SFC column chemistries, with little change in the retention of flavone.
www.waters.com
Viridis SFC Columns
197
[ Bioseparations and Analyses ]

Introduction ...........................................................................................................................................................................................200
Peptide Analysis and Isolation
Peptide Isolation and Analysis ................................................................................................................................................202
Peptide Separation Technology ...............................................................................................................................................204
PST Therapeutic Peptide Method Development Kit .................................................................................................................209
ACQUITY HSS UPLC and Atlantis T3 HPLC Columns .............................................................................................................210
BioSuite HPLC Peptide Analysis Columns...............................................................................................................................212
BioSuite Cation-Exchange HPLC Columns ...............................................................................................................................213
Delta-Pak HPLC Columns ..........................................................................................................................................................213
Waters Insulin HMWP HPLC Column ........................................................................................................................................213
Symmetry HPLC Columns.........................................................................................................................................................214
Protein Isolations and Analyses
Protein Isolation and Characterization ....................................................................................................................................215
ACQUITY UPLC SEC System Solution ......................................................................................................................................217
Protein Separation Technology (BEH 300, C4).......................................................................................................................221
Protein-Pak Hi Res Ion-Exchange (IEX) columns
For Acquity UPLC Applications ................................................................................................................................................223
BioSuite Ion-Exchange HPLC Columns .....................................................................................................................................226
Protein-Pak HR Ion-Exchange
Glass Columns ..........................................................................................................................................................................227
Protein-Pak PW Series Columns ...............................................................................................................................................227
Advanced Purification (AP) Glass Column
Accessories and Spare Parts ....................................................................................................................................................228
Fittings and Connectors for Advanced
Purification (AP) Glass Columns ..............................................................................................................................................229
Accell Plus Ion-Exchange Packings ..........................................................................................................................................230
BioSuite Size-Exclusion HPLC Columns (SEC) .........................................................................................................................231
Protein-Pak and Shodex
Size-Exclusion HPLC Columns (SEC) ........................................................................................................................................234
Ultrahydrogel HPLC Columns...................................................................................................................................................234
Symmetry300 C4 HPLC Columns ............................................................................................................................................235
Protein-Pak Affinity Columns ..................................................................................................................................................235
BioSuite pC18 and pPhenyl Reversed-Phase
Chromatography (RPC) HPLC Columns .....................................................................................................................................236
BioSuite Hydrophobic-Interaction
Chromatography (HIC) HPLC Columns .....................................................................................................................................237
Protein HIC and Protein-Pak Phenyl HIC
HPLC Columns ...........................................................................................................................................................................237
198
Amino Acid Analysis

Amino Acid Analysis ...............................................................................................................................................................238
UPLC Amino Acid Analysis Solution ......................................................................................................................................239

AccQTag Amino Acid Analysis Using HPLC.........................................................................................................................241
PicoTag HPLC Method .............................................................................................................................................................242

MassTrak Amino Acid Analysis Solution ...............................................................................................................................243
Oligonucleotide Separation Technology

Oligonucleotide Purification and Analysis .............................................................................................................................244
Oligonucleotide Separation Technology .................................................................................................................................245
Columns for Large DNA/RNA Species .....................................................................................................................................248
Gen-Pak FAX Anion-Exchange Columns ..................................................................................................................................248
MassPREP OST Standard ...........................................................................................................................................................249
Oasis Elution Plates................................................................................................................................................................249
Glycan Analysis
Glycan Separation Technology ................................................................................................................................................250
ACQUITY UPLC BEH Glycan Columns ......................................................................................................................................251
MassPREP Glycoanalysis Kit.....................................................................................................................................................252
MS and LC/MS Consumables for Biomolecules
MS and LC/MS Consumables
for Biomolecules .......................................................................................................................................................................253
RapiGest SF and MassPREP Oligonucleotide,
Peptide, and Protein Digest Standards....................................................................................................................................254
MassPREP Peptide Standards ...................................................................................................................................................254
MassPREP Protein Digestion Standards ...................................................................................................................................254
MassPrep Protein Standard Mix ...............................................................................................................................................255
MassPREP Phosphopeptide Standards ......................................................................................................................................255
MassPREP Digestion Standard Mixtures ..................................................................................................................................256
MassPREP OST Standard ...........................................................................................................................................................256
RapiGest SF Protein Digestion Surfactant ...............................................................................................................................257
MassPREP On-Line Desalting Devices .....................................................................................................................................258
NanoEase Trap, Capillary, and Nano Columns........................................................................................................................259
NanoEase Nano and Capillary Columns ..................................................................................................................................260
nanoACQUITY UltraPerformance LC Columns ........................................................................................................................261
nanoACQUITY UPLC 2D Kit......................................................................................................................................................262
MassPREP Phosphopeptide Enrichment Kit ..............................................................................................................................263
Waters Protein Expression System Kits...................................................................................................................................263
UPLC Technology for Biomolecules.........................................................................................................................................................264
199
bIOSEPaRaTIONS aNd aNaLYSES

Innovative Technologies From the Leaders in Separation
Science and Analytical Biochemistry
Advances in the areas of genomics, proteomics, metabanomics, and

molecular and system biology continue to revolutionize the diagnosis
and treatment of diseases and increase our fundamental understanding of
biological processes.
As a leading analytical supplier of instrumentation, software, service and
support, and chemistry products, Waters is uniquely positioned to provide
researc hers the tools, tec hnologies, and integrated solutions desired
to tackle the formidable c hallenges involving various biomolecules.
Beginning with a keen understanding of todays biomolecule-related
challenges, Waters scientists and engineers continuously seek purposeful
innovations that help deliver impactful solutions in applications ranging
from proteomics and biomarker discovery through the commercialization
of advanced biopharmaceuticals. We continue to develop new, innovative
columns and sample preparation consumables that support the HPLC,
UPLC tec hnology, and LC/MS analyses of peptides, oligonucleotides,
proteins, amino acids, and glycans.
200
Waters comprehensive chemistry and consumables family includes:
Peptide Separation Technology columns for nano, capillary,

analytical, and preparative peptide applications
Protein Separation Technology reversed-phase columns, BioSuite, SEC,

IEX, RP, and HIC columns for analytical and purification applications
AccQTag
Ultra chemistry specific for Waters UPLC Amino Acid

Analysis Solution, as well as PicoTag and AccQTag for HPLC-based
amino acid analyses
Oligonucleotide Separation Tec hnology columns for synthetic

oligonucleotide and DNA/ RNA fragment isolations and analyses
Glycan Separation Tec hnology sample prep and UPLC columns

for the analysis of 2-AB labeled Glycans
MassPREP c hemistry consumables and kits for MS and LC/MS

applications of peptides, proteins, and other biomolecules
BIOSEPaRatIOnS and analySES
Peptide and Oligonucleotide Isolations and Analyses

Synthetic peptides and oligonucleotides can be effectively separated by various c hromatographic techniques including reversed-phase, ion-exchange, and size-exclusion chromatography.
Waters Peptide Separation Technology and BioSuite columns are designed to address various
peptide analysis and lab-scale purification needs. Our Oligonucleotide Separation Technology
reversed-phase and GenPak Fax IEX columns address various high-resolution analysis and
lab-scale isolation challenges involving various DNA and RNA species.
Amino Acid Analysis

Waters solutions in this area have evolved from more than 25 years of experience in amino acid
analysis and include both HPLC and UPLC-based offerings. AccQTag and PicoTag pre-column
derivatization chemistries and columns are designed for traditional HPLC applications. In 2006,
we introduced our UPLC Amino Acid Analysis Solution that uses our next generation AccQTag
Ultra chemistry to provide superior component resolution and separation speed compared to
results possible using established HPLC-based methods.
Protein Analysis and Characterization

As the major building blocks of life, many biopharmaceutical advances have resulted from the
detailed knowledge and characterization of these molecules. Consequently, detailed protein
characterization studies are necessary that incorporate powerful analytical techniques, such as
peptide mapping and mass spectrometry using a variety of HPLC and UPLC column chemistries
that include size exclusion, ion exchange, hydrophobic interaction, and reversed phase. Waters
Protein Separation Technology reversed-phase, BioSuite, SEC, IEX, HIC, and RP HPLC columns,
offer outstanding performance when applied to the separation of these macromolecules. In
addition our new Protein-Pak Hi Res IEX and ACQUITY UPLC BEH200 SEC columns were developed for use on Waters ACQUITY UPLC Instrumentation to help you obtain accurate, precise,
and high-throughput quantitative analysis of therapeutic proteins such as mAbs.
Proteomics, Protein Expression, and Biomarker Discovery

Experiments in proteomics begin with samples that are both very complex and limited in
volume and concentration. Good sample preparation and separation of the various analytes
prior to MS analysis can greatly enhance detection sensitivity and provide more meaningful
results. Waters MS and LC/MS consumables that include NanoEase and nanoACQUITY trap,
capillary, and nano columns, RapiGest SF reagent, as well as MassPREP standards and kits
help ensure the best results from MS and LC/MS instrumentation.
Glycan Analysis
Glycoprotein analysis can involve identifying complex N and 0-Linked structures composed of
frequently similar and repeating sugar moieties. Hydrophilic-interaction liquid chromatography
(HILIC) with fluorescence detection is a well-recognized and reliable technique that effectively
separates and quantitates isolated glycans after their derivatization with fluorescent labels.
Waters ACQUITY UPLC BEH Glycan column was specifically developed and QC tested to provide
superior UPLC component resolution in less time for a range of glycan structures. W hen coupled
to Waters ACQUITY UPLC system, our column chemistry is ideally suited to help users get the
correct answers faster.
www.waters.com
201
PEPTIdE ISOLaTIONS aNd aNaLYSES
Peptide Analysis and Isolation

T he separation of peptides plays a fundamental role in many areas of
researc h and development. Some peptides have significant biological
activity and require analysis in a complex matrix. It may be necessary to
isolate these molecules as natural products or as synthetic peptides. In
addition, the separation of peptides is a fundamental tool for the analysis
of protein structure because the small, reproducible fragments are a tool
for dissecting the structural details of the much larger protein. T he same
principle underlies the use of peptide separations for analyzing proteomics, protein expression, and biomarker discovery. All of these applications
focus on a family of molecules with similar properties suc h that the
separation can be approac hed in the same way. T he principles behind
each separation mechanism can be considered in selecting a column for
a particular application. T he choice of chromatographic mode will depend
on the type of peptide sample you are separating. T he guidelines below
will help direct you to the appropriate column selection.
Reversed-Phase Peptide Separations
Mechanisms
Reversed-phase separations generally give the highest resolution of
peptide mixtures, often allowing for effective separation of peptides
differing by a single amino acid. In reversed-phase separations, the
hydrophobicity of the peptide determines the elution order, with the least
hydrophobic peptides eluting first. T his hydrophobicity is related to the
size of t he peptide, wit h larger molecules being more hydrophobic
in general, and also to the hydrophobicity of the side c hains of the
constituent amino acids. Both the retention and the separation selectivity
are also influenced by the three-dimensional structure of the peptide.
T he packing material interacts with these peptide properties based
foremost on the hydrophobicity of the column surface, reflecting bonded
phase chain length and density. Other column properties that can strongly
influence the separation selectivity include the pore size of the packing
material and the surface chemistry of the particle of the packing material.
best retention and solubility for peptides. It also gives the best peak
shape since the peptide basic groups are shielded from secondary interactions with the surface of the packing material.
TFA, however, reduces the sensitivity of MS detection of peptides. W hile
usable signal is usually obtained by using lower concentrations of TFA,
many investigators prefer to use formic acid as the mobile phase modifier.
In this case, the column surface has a larger effect on the separation.
Retention is often muc h less, and many of the peptide peaks will be
asymmetrical and tailing. It is common practice to choose columns designated as no TFA or low TFA for these separations. T he BioSuite PA-A
column described below was developed for this purpose. More recently the
development of Ethylene-Bridged Hybrid (BEH Technology) packing materials provided an improved option. T he ethane bridges reduce the surface
concentration of silanol groups so that there is little secondary interaction
between peptides and the base particle. Packing materials based on these
particles can be used for peptide separations in either T FA or formic
acid containing mobile phases without significant loss of retention or
band-broadening. T he BEH Tec hnology particles for the basis of the
Peptide Separation Technology family of columns described below.
With the Peptide Separation Technology columns, the minimal secondary
interactions create the greatest number of options for adjusting selectivity
with mobile phase manipulation. T he selectivity of a peptide separation
is different for mobile phases containing TFA and formic acid. T he use of
columns that give good performance with either modifier create an option
for developing methods in addition to the desirable impact on sensitivity
in MS detection. Furthermore, because peak shape and resolution are not
dependent on the presence of TFA, the concentration of TFA can be adjusted
to fine tune the selectivity of a peptide separation.
More dramatic changes in selectivity can be induced by changing the pH
of the separation from low pH to pH 7 or even pH 10 (e.g., using XBridge
BEH130 C18 columns).
Column Selectivity
Ion-Exchange Chromatography
T he retention mechanism of peptide separations is similar on reversedphase HPLC columns in general. T he selectivity of the separation is
significantly affected by the base sorbent, pore size, alkyl ligand
density, and residual silanol endcapping. T hese small differences in
separation selectivity can be a part of developing a peptide separation. If it is difficult to ac hieve a desirable separation on a particular
R P-HPLC column, then the use of an alternative R P-HPLC column
chemistry may be tested. For example, different separation selectivities
will be observed using XBridge BEH300 C18 compared to Atlantis T3.
In ion-exc hange c hromatography, the c harge on the amino acid side

c hains is the determining factor in the separation. T he benefits of
ion-exc hange c hromatography are the high-capacity packings and the
ability to easily load ion-exc hange fractions onto a reversed-phase
column for desalting or final purification. W hile ion-exc hange separations of peptides are not generally as highly resolving as reversed
phase, the tec hnique can provide an orthogonal selectivity for difficult
samples. Peptides most often separate best on strong cation exc hangers.
T he ion-exc hange column packings described in the section on protein
c hromatography are suitable for peptides.
Mobile-Phase Selectivity
Peptides are c harged molecules that do not freely interact with the
hydrophobic surface of reversed-phase columns. T he mobile phase
is typically, therefore, acidified to protonate the peptide carboxyl
groups, increasing retention. Trifluoroacetic acid is often preferred for
this purpose because it also forms ion pairs with the basic groups of
peptides. T he combination of protonation and ion pairing leads to the
202
Peptide Isolations and Analyses
Size-exclusion chromatography is sometimes used for peptide separations.
T he peptides are separated based on their size in solution. T he resolution
seldom compares favorably to either reversed phase or ion exchange, but
it can provide an alternative for some samples. T he composition of the
separation buffer will require careful adjustment for each particular peptide
www.waters.com
sample. Elevated ionic strength and the addition of some organic solvent,
typically methanol or propanol, may be necessary for good peak shape. T he
size-exclusion packings, particularly those with smaller pore sizes, described
in the section on protein chromatography, are suitable for peptides.
Applications of Peptide Separations

Peptide Mapping
Peptide mapping continues to be the preferred technique for the comprehensive characterization of biopharmaceutical products and proteins in general.
Waters provides an array of products that facilitate the development of
information-rich peptide maps. Before applying the separation techniques
discussed above, the purified protein sample must be digested. T his step
often proves difficult because of incomplete and irreproducible digestion,
non-specific cleavages, precipitation of large core peptides, and slow
digestion. RapiGest SF is a surfactant that unfolds proteins so that the sites
of enzymatic cleavage are freely accessible for digestion. T his improves
reproducibility and speed. It also helps to keep all the sample components
in solution throughout the digestion process and up to the analysis. T he
properties and use of RapiGest are fully described below in the section on
MS and LC/MS consumables. T his section also describes several standard
mixtures of peptides and protein digests that are used to test and confirm
the performance of columns and instruments before committing any
samples to the peptide mapping system.
T he primary c hoice for the separation component of peptide mapping
is the Peptide Separation Tec hnology family of columns. T his packing
material is based on BEH Tec hnology particles to minimize secondary interactions and to maximize column lifetime. T hese materials
are available with either 130 or 300 pores to adapt to the sizes
of peptides being analyzed. Both pore sizes are available in a range
of column dimensions suited to the sample size. T he same separation
c hemistry is provided with either 3.5 m particles for HPLC analysis or
with 1.7 m for use with ACQUIT Y UPLC systems. Peptide Separation
Tec hnology columns are specifically QC-tested with protein digests
to confirm batc h to batc h reproducibility. T his column c hemistry is,
therefore, suitable for the development of methods that are to be used
routinely for extended periods.
BioSuite PA-A column is especially suitable for polar peptides and for
use with MS-compatible eluents. T he BioSuite PA-B column has 300
pores for the analysis of large and hydrophobic peptides. Both BioSuite
Peptide Analysis columns are specifically QC-tested with protein digests
to ensure batc h-to-batc h product consistency and column-to-column
performance reproducibility.
Many other Waters columns have been used for particular peptide mixtures.
It is often observed that most peptides will give reasonable chromatography
on many different columns. T here is, however, often a particular column
that, for some subtle and unpredictable reason, works better than any other
for a particular pair of peptides.
Isolation of Natural and Synthetic Peptides
Purification of large amounts of individual peptides is most often achieved
with reversed-phase chromatography using the same principles and ideas
described above. T he columns are often c hosen to provide economical
separations on a larger scale so they are packed with larger size particles.
T he Peptide Separation Technology columns described above are available
in larger particle sizes and larger dimension columns for this purpose. T he
minimal secondary interactions mean that these columns can be used for
peptides representing a wide range of sizes and chemical properties. T he
surface chemistry of these packings is consistent and scalable across the
range of particle sizes. As necessary, separations may be optimized on
small scale columns and then transferred to columns large enough for the
required sample.
For some complex mixtures, two step separations may give the highest yield
and purity. In general, the best first step is cation-exchange chromatography
followed by a reversed-phase step for final isolation and desalting.
Proteomics, Analysis of Protein Expression, and Biomarker Discovery
For analyses used in proteomics and biomarker discovery, the available
samples are extremely limited so the requirements of c hromatographic
separation must be coupled with extreme sensitivity. T he sample
c hromatographic tools and principles described above are implemented
on the smallest scale in the nanoACQUIT Y UPLC system with nanoACQUIT Y columns. T he application solutions are described below in the
section on MS and LC/MS consumables.
Other Waters columns also have proven suitable for peptide mapping.
T he BioSuite Peptide Analysis columns are two premier reversed-phase
column c hemistries specifically optimized for peptide mapping. T he
www.waters.com
203
Peptide Separation Technology

Unique new columns that meet the demanding requirements of
peptide separations:
Improved chromatography of peptides

- Narrow, symmetrical peaks for best resolution
- Successful separations of a wide range of peptides: large and
small, acidic and basic, hydrophobic and hydrophilic
- Good peak shape and retention in formic acid and trifluoroacetic
acid (TFA) for optimal chromatography and detection
- Available in 130 and 300 pore sizes for varying
sample requirements
Wide range of particle sizes and column dimensions for consistent

separations in applications from high sensitivity proteomics to high
mass load purification
Unique column chemistry combined with

the benefits of sub-2 m particles of UPLC
for the ultimate resolution
A Reversed-Phase Offering for a Broad Range of Peptides
0.70
0.36
0.60
0.32
Average
0.24
0.20
AU
AU
0.40
Basic
pI 10.3
15 Residues
1809 da
0.28
pI 6.4
17 Residues
1772 da
0.50
0.30
0.16
0.12
0.20
0.08
0.10
0.04
0.00
0.00
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
15
90 min
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90 min
60
65
70
75
80
85
90 min
0.70
Large
0.60
Hydrophobic
0.32
pI 4.7
~
~40 Residues
~
~4000 da
0.50
pI 7.3
16 Residues
1872 da
0.28
0.24
0.40
AU
AU
0.20
0.30
Peptide
0.16
0.12
0.20
0.08
Preservative
0.10
0.04
0.00
0.00
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90 min
15
20
25
30
35
40
45
50
55
PST columns are compatible with a broad range of peptides. A single column can be used to purify the wide range of sample types generated in a peptide
synthesis laboratory. This minimizes the need to switch for columns separating peptides of extreme sizes, isoelectric point, or hydrophobicity.
204
www.waters.com
Two Pores Sizes for Flexible Method Development

C
1.2e-1
4 residue
25 residue
20 residue
507.3
2447.2
1565.8
1.0e-1
8.0e-2
AU
A
B
C
6.0e-2
4.0e-2
2.0e-2
0.0
20
25
30
35
40
45
50
55
60
65
70
75
80 min
75
80 min
6.0e-2
5.0e-2
AU
4.0e-2
Comparison of the separation of tryptic

digest of phosphorylase b on ACQUITY UPLC
BEH130 and BEH300 1.7 m Peptide
Separation Technology columns.
3.0e-2
2.0e-2
1.0e-2
0.0
20
25
30
35
Reversed-Phase Peptide Mapping

for Protein Characterization
Peptide Separation Technology columns are based on synthetic particles that
ensure the highest quality and most consistent performance. T his second
generation of Waters patented BEH Technology is a result of Waters commitment to continued investment in c hromatography researc h and development. T hey not only meet the demanding manufacturing and performance
specifications for the BEH Technology particles, but, for the first time, are
also specifically QC tested with a peptide map.
Well characterized, state-of-the-art bonding procedures for C18 ligand
Particle structure and bonding chemistry stable at pH 1 to 12 and at

elevated temperature
Quality-control tested with a complex protein digest peptide map
Consistent batch-to-batch synthetic peptide or protein digest separation
www.waters.com
40
45
50
55
60
65
70
ACQUITY UPLC and XBridge BEH130

and BEH300 C18 Columns Certificate
of Application SpecificAnalysis
Each batch of ACQUITY UPLC and XBridge BEH130 and 300 C18 column
material is rigorously tested to ensure consistent performance. T he
Certificate of Analysis available for each column reports physical, chemical, and chromatographic tests obtained using a tryptic digest of bovine
cytochrome c.
205

Simplifying Column Choice for
Peptide Purifications
Long-term Stability
100
Peptide Separation
Technology
B
90
C
% Initial Retention
A benefit of Waters PST columns is the frequent ability to use a single,

C18-based chemistry to separate a wide range of peptides as indicated in
analytical separations on page 205. T his commonly translates into less
time and expense before an appropriate peptide purifcation separation is
obtained. PST packings are available in a wide range of particle sizes and
column dimensions, all with the same, consistent chemistry for various
needs as indicated in accompanying preparative column selection guide.
80
D
70
60
Separation of 13 Residue Peptide at Various Sample Loads
50
50
100
Several columns were

tested with repeated
injections. Retention was
monitored to determine
column lifetime.
Hours in 1% TFA in H2O pH 1.0 at 80 C

2.6e-1
2.4e-1

1.7 m, 2.1 x 100 mm
200 pmol on column
2.2e-1
2.0e-1
Peptide Separation Technology Preparative Column Selection Guide
AU
1.8e-1
1.6e-1
Length
(mm)
50
100
150
250
50
100
150
250
OBD Prep Columns, 5 m and 10 m

130 and 300
moles of a
Weight of a Single
Single Peptide
Peptide (mg)
0.25 - 5
0.5 - 10
0.25 - 5
0.5 - 10
0.25 - 5
0.5 - 10
0.25 - 5
0.5 - 10
1 - 18
2.0 - 36
1 - 18
2.0 - 36
1 - 18
2.0 - 36
1 - 18
2.0 - 36
Typical Flow Rate

(mL/min)
4.5 - 9
4.5 - 9
4.5 - 9
4.5 - 9
16 - 32
16 - 32
16 - 32
16 - 32
Length
(mm)
50
100
150
250
OBD Prep Columns, 10 m

130 and 300
moles of a
Weight of a Single
Single Peptide
Peptide (mg)
2.5 - 25
5 - 100
2.5 - 25
5 - 100
2.5 - 25
5 - 100
2.5 - 25
5 - 100
Typical Flow Rate

(mL/min)
40 - 80
40 - 80
40 - 80
40 - 80
1.4e-1
1.2e-1
1.0e-1
8.0e-2
30
32
34
36
38
40
42
44
46
48
50 min
1.5
XBridge BEH130 C18

5 m, 4.6 x 50 mm
40 gs on column
1.3
AU
1.1
9.0e-1
7.0e-1
5.0e-1
3.0e-1
i.d.
(mm)
10
10
10
10
19
19
19
19
1.0e-1
2
5.25e-2
10
11
12 min
i.d.
(mm)
30
30
30
30
XBridge BEH130 ODB Prep C18

5 m, 19 x 100 mm
3.4 mg injected
4.75e-2
4.25e-2
3.75e-2
AU
3.25e-2
2.75e-2
2.25e-2
1.75e-2
1.25e-2
7.5e-3
2.5e-3
4
10
11
12
13
14
15
16 17 min
Waters PST columns, available in different particle size and column configurations,
are well-suited for various lab-scale purification needs.
PST Packing Material in OBD Columns for

Maximum Chemical and Physical Stability
Columns fail both physically and chemically. T he predominant cause of short
column lifetime in low pH mobile phases is hydrolysis of the bonded phase
that leads to significant c hanges in peptide retention. BEH Tec hnology
columns incorporate proprietary procedures for bonding and endcapping
that yield stable bonded phases. In low pH stability tests, BEH C18 columns
showed very little retention loss. T he physical stability of these columns is
ensured by the optimum bed density (OBD) design that was developed to
produce the most stable packed beds. For more information on OBD technology, visit www.waters.com.
206
Cation-Exchange Peptide and

Polypeptide Separations
For most analytical and preparative peptide separations, cation-exchange
chromatography is used mainly when alternative selectivity is required. In
some large-scale purifications, cation exchange can take on a more central
role. In these cases, cation exchange is frequently used as the first step in
the separation, followed by a secondary purification step using reversedphase methods.
Waters offers Protein-Pak SP HR packings for cation-exc hange separations. T hese packings are useful both for analytical and preparative work.
T hey are based on rigid, hydrophilic polymethacrylate particles with large
1000 pores. T he naturally hydrophilic polymer reduces non-specific
adsorption, resulting in better recovery of peptide/polypeptide mass and
bioactivity. T hese packings are stable in the pH range of 2-12.
Protein-Pak SP HR 8 and 15 m packing material is available in prepacked
glass columns.
www.waters.com
PEPTIDE ISOLATIONS AND ANALYSES

Peptide Nano and Capillary Columns
Peptide Separation Technology nanoACQUITY UPLC
Columns (10,000 psi)
Description
Inner
Diameter
nanoACQUITY UPLC BEH130 C18

75 m
75 m
75 m
75 m
100 m
150 m
75 m
100 m
150 m
75 m
75 m
75 m
Peptide Separation Technology ACQUITY UPLC Columns
Length
Particle
Size
Part No.
100 mm
150 mm
200 mm
250 mm
100 mm
100 mm
100 mm
100 mm
100 mm
150 mm
200 mm
250 mm
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
186003542
186003543
186003544
186003545
186003546
186003550
186003810
186003811
186003812
186003813
186003814
186003815
Peptide Analytical Columns

Peptide Separation Technology NanoEase Columns
Description
Inner
Diameter
Length
Particle
Size
Part No.
XBridge BEH130 C18

XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH130 C18
75 m
75 m
75 m
100 m
100 m
100 m
150 m
150 m
150 m
300 m
300 m
300 m
75 m
75 m
75 m
100 m
100 m
100 m
150 m
150 m
150 m
300 m
300 m
300 m
300 m
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
186003588
186003589
186003590
186003591
186003592
186003593
186003594
186003595
186003596
186003597
186003598
186003599
186003632
186003633
186003634
186003635
186003636
186003637
186003638
186003639
186003640
186003641
186003642
186003643
186003682
*NOTE: Use of Waters 300 m Capillary Inlet Tubing (Part Number 430001471) and 300 m Capillary Outlet Tubing (Part Number
430001472) required for use with 300 m I.D. capillary columns. Waters Kit Part Number 700002757 contains both the 300 m
Capillary Inlet Tubing (Part Number 430001471) and the 300 m Capillary Outlet Tubing (Part Number 430001472).
Peptide Separation Technology Methods Validation Kits**

Description
Inner
Diameter
Length
Particle
Size

XBridge BEH130 C18
XBridge BEH300 C18
2.1 mm
2.1 mm
4.6 mm
4.6 mm
100 mm
100 mm
100 mm
100 mm
1.7 m
1.7 m
3.5 m
3.5 m
**Three columns from three different batches of material
www.waters.com
Part No.
186004896
186004897
186004904
186004905
Description
Inner
Diameter
Length
Particle
Size
Part No.

2.1 mm
2.1 mm
2.1 mm
2.1 mm
2.1 mm
2.1 mm
1.0 mm
1.0 mm
1.0 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
186003554
186003555
186003556
186003685
186003686
186003687
186005592
186005593
186005594
ACQUITY UPLC VanGuard Pre-Column and Column In-Line Filter Unit

Description
Part No.
BEH300, C18, 1.7 m, 2.1 x 5 mm, 3/pk

BEH130, C18, 1.7 m VanGuard Pre-Column, 2.1 x 5 mm, 3/pk
In-line filter holder and six 0.2 m
stainless steel replacement filters
186004629
186003975
205000343
700002775
Peptide Separation Technology HPLC Columns

Description
Inner
Diameter
Length
Particle
Size
Part No.
XBridge BEH130 C18

XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
1 mm
1 mm
1 mm
2.1 mm
2.1 mm
2.1 mm
2.1 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
1 mm
1 mm
1 mm
2.1 mm
2.1 mm
2.1 mm
2.1 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
1 mm
1 mm
1 mm
2.1 mm
2.1 mm
2.1 mm
2.1 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
1 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
186003560
186003561
186003562
186003563
186003564
186003565
186003566
186003567
186003568
186003569
186003570
186003571
186003572
186003573
186003574
186003575
186003576
186003577
186003578
186003579
186003580
186003581
186003604
186003605
186003606
186003607
186003608
186003609
186003610
186003611
186003612
186003613
186003614
186003615
207

Peptide Preparative Columns
Peptide Separation Technology Guard Columns
Peptide Separation Technology HPLC Columns

Description
Inner
Diameter
Length
XBridge BEH300 C18

XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
1 mm
1 mm
2.1 mm
2.1 mm
2.1 mm
2.1 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
10 mm
10 mm
10 mm
10 mm
19 mm
19 mm
19 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
10 mm
10 mm
10 mm
10 mm
19 mm
19 mm
19 mm
30 mm
30 mm
30 mm
30 mm
10 mm
10 mm
10 mm
10 mm
19 mm
19 mm
19 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
10 mm
10 mm
10 mm
10 mm
19 mm
19 mm
19 mm
30 mm
30 mm
30 mm
30 mm
100 mm
150 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
50 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
208
Particle Configuration
Size
Style
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
Column
OBD Column
OBD Column
OBD Column
Column
Column
Column
Column
Column
Column
Column
Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
Column
Column
Column
Column
OBD Column
OBD Column
OBD Column
Column
Column
Column
Column
Column
Column
Column
Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
Part No.
186003616
186003617
186003618
186003619
186003620
186003621
186003622
186003623
186003624
186003625
186003582
186003583
186003584
186003585
186003586
186003587
186003945
186003648
186003649
186003650
186003651
186003652
186003653
186003654
186003655
186003656
186003657
186003658
186003659
186003660
186003661
186003662
186003626
186003627
186003628
186003629
186003630
186003631
186003946
186003663
186003664
186003665
186003666
186003667
186003668
186003669
186003670
186003671
186003672
186003673
186003674
186003675
186003676
186003677
Description
Inner
Diameter
Length
Particle Configuration
Size
Style
XBridge BEH130 C18

XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH130 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
XBridge BEH300 C18
10 mm
19 mm
10 mm
19 mm
10 mm
19 mm
10 mm
19 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
5 m
5 m
10 m
10 m
5 m
5 m
10 m
10 m
Guard Column
Guard Column
Guard Column
Guard Column
Guard Column
Guard Column
Guard Column
Guard Column
Part No.
186004469
186004468
186004465
186004464
186004471
186004470
186004467
186004466
Peptide Separation Technology OBD Columns

BEH130 and BEH300 C18 OBD Prep Columns, 5 m and 10 m
Inner
Diameter
Length
moles of a
Single Peptide
Weight of a Single
Peptide (mg)
Typical Flow Rate

(mL /min)
10 mm
10 mm
10 mm
10 mm
19 mm
19 mm
19 mm
19 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
0.25-5
0.25-5
0.25-5
0.25-5
1-18
1-18
1-18
1-18
0.5-10
0.5-10
0.5-10
0.5-10
2.0-36
2.0-36
2.0-36
2.0-36
4.5-9
4.5-9
4.5-9
4.5-9
16-32
16-32
16-32
16-32
Description
Qty.
Replacement o-ring 7.8 mm
Replacement o-ring 10 mm
Part No.
289000779
186000709
2/pkg
2/pkg
2/pkg
700001019
700001436
700001020
www.waters.com

T he PST T herapeutic Peptide Method Development Kit has been developed
to simplify the process of sample preparation and LC method development
for the analysis of therapeutic peptides in plasma. T he kit contains an Oasis
PST Elution Method Development Plate, a PST 300 C18 reversed-phase
column and the detailed screening protocol which was used to generate the
data shown in this publication.
In addition, a comprehensive method development training seminar has been
created which describes all aspects of the method development process from
the MS conditions to the final validation of a method for the extraction of
the therapeutic peptide Desmopressin from human plasma.
For more information, visit www.waters.com/pepkit,
or contact your local Waters sales office.

Description
UPLC PST Therapeutic Peptide Method Development Kit
Includes:
Oasis PST Elution Method Development Plate
ACQUITY UPLC BEH 300 C18 2.1 x 50 mm, 1.7 m
96-well 1 mL Collection Plate and Cap Mat
HPLC PST Therapeutic Peptide Method Development Kit
Includes:
Oasis PST Elution Method Development Plate
XBridge BEH 300 C18 2.1 x 50 mm, 3.5 m
96-well 1 mL Collection Plate and Cap Mat
Part No.
Qty/Box
176001835
186004713
186003685
600001043
1
1
3
176001836
Peptide Separation Technology Brochure,

186004713
186003607
600001043
1
1
3
186001829
186002499
186002481
186002483
WAT058942
186001831
186003522
725000417
725000418
1
1
50
50
25
1
2
1
1
Available Waters Products Not Included in Kit:

Oasis MAX 96-well Elution Plate
Oasis WCX 96-well Elution Plate
96-well 1 mL Collection Plate
Cap Mats for 1 mL Collection Plate
Disposable Reservoir Tray
Extraction Manifold for 96-well Plates
Vacuum Box Gasket Kit (includes foam top gaskets and orange O-rings)
SPE Vacuum Pump 115 V, 60 Hz
SPE Vacuum Pump 240 V, 50 Hz
www.waters.com
Literature
References
UPLC Peptide Analysis Solution Brochure,

Optimum Bed Density (OBD) Preparative
Column Technology Brochure,
Synthetic Peptide Purification
Solutions Brochure,
209
PePtide isolations and analyses

Waters ACQUITY HSS UPLC and Atlantis T3 C18 HPLC Columns for Peptide Separations
T he PST family of reversed-phase columns was developed to provide good
resolution for both the analysis and lab-scale isolation of peptides. In
addition to these offerings, Waters ACQUITY HSS UPLC and Atlantis
T3 C18 HPLC offerings (100 pores) are useful when alternative column
c hemistries that ex hibit different peptide separation selectivities are
required for a particular application.
T he Atlantis T3 C18 series off analytical and preparative columns, available

in 3-10 m particles, is well suited for HPLC-based peptide analysis and
purification needs. Waters ACQUITY UPLC HSS T3, HSS C18, and HSS C18
SB columns, when combined with the UPLC Instrumentation, may provide
resolution and speed of analysis similar to that obtained with our BEH130
or BEH300, C18 UPLC columns.
Peptide Separations on Waters BEH and Silica-Based C18 Column Offerings
2.1 x 100 mm
Sample:
30 pmoles MassPREP phosphorylase digestion standard
Detection:
ESI+; Waters Q-Tof Premier
Run Time:
70 min
Temperature:
40 C
Solvent A:
0.1% Formic Acid in Water
Solvent A:
0.1% Formic Acid in Acetonitrile
Gradient:
Time(min)
Flow Rate
%A
%B
Initial
0.200
95.0
5.0
1.00
0.200
95.0
5.0
59.00
0.200
50.0
50.0
60.00
0.200
30.0
70.0
61.00
0.200
95.0
5.0
Curve
ACQUITY UPLC HSS T3 C18
Columns:
210
10
15
20
25
30
35
40
45 mIn
www.waters.com
PePtide isolations and analyses

Atlantis T3 C18 HPLC and ACQUITY HSS UPLC Analytical Columns
Inner
Diameter
1.0 mm
1.0 mm
1.0 mm
2.1 mm
2.1 mm
2.1 mm
3.0 mm
3.0 mm
3.0 mm
1
2
Length
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
Configuration
Style
Column
Column
Column
Column
Column
Column
Column
Column
Column
Atlantis T3
C18, 3 m
186003713
186003714
186003717
186003718
186003719
186003721
186003722
186003723
C18, 5 m
186003730
186003731
186003734
186003735
186003736
186003738
186003739
186003740
ACQUITY HSS
T3, 1.8 m
C18, 1.8 m
C18 SB, 1.8 m
1 pack
3 pack
1 pack
3 pack
1 pack
3 pack
186003535 176001127 186003529 176001121 186004114 176001556
186003536 176001129 186003530 176001122 186004115 176001557
186003537 176001130 186003531 176001123 186004116 176001558
186003538 176001131 186003532 176001124 186004118 176001560
186003539 176001132 186003533 176001125 186004119 176001561
186003540 176001133 186003534 176001126 186004120 176001562
186004679 176001814 186004683 176001818 186004687 176001822
186004680 176001815 186004684 176001819 186004826 176001823
186004681 176001816 186004685 176001820 186004689 176001824
Inner
Diameter
Length
Configuration
Style
Particle
Size
Part No.
10 mm
10 mm
10 mm
10 mm
10 mm
19 mm
19 mm
19 mm
19 mm
19 mm
30 mm
30 mm
30 mm
30 mm
50 mm
50 mm
50 mm
10 mm
10 mm
10 mm
19 mm
19 mm
19 mm
19 mm
30 mm
30 mm
50 mm
50 mm
50 mm
50 mm
10 mm
50 mm
100 mm
150 mm
250 mm
10 mm
50 mm
100 mm
150 mm
250 mm
50 mm
75 mm
100 mm
150 mm
50 mm
100 mm
150 mm
10 mm
150 mm
250 mm
10 mm
50 mm
150 mm
250 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
Guard
Column
Column
Column
Column
Guard
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
Guard
Column
Column
Guard
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
OBD Column
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
10 m
1860036951
186003691
186003692
186003693
186003694
1860036992
186003696
186003697
186003698
186004026
186003700
186003701
186003702
186003703
186004080
186004081
186004082
1860037061
186003704
186003705
1860037102
186003707
186003708
186003709
186003711
186003712
186004083
186004084
186004085
186004086
ACQUITY HSS
Requires Sentry Guard Holder WAT097958

Requires Sentry Guard Holder WAT046910
Atlantis T3 C18 Prep Columns for Peptides
ACQUITY HSS
Description
Qty.
Replacement o-ring 7.8 mm
Part No.
289000779
186000709
2/pk
2/pk
2/pk
700001019
700001436
700001020
Requires 10 x 10 mm Prep Guard Holder 289000779

Requires 19 x 10 mm Prep Guard Holder 186000709
www.waters.com
211
BioSuite HPLC Peptide Analysis Columns
Two HPLC column chemistries that provide alternative

chemistries for peptide separations
Designed for maximum resolution of complex digests
Available in various configurations for LC or LC/MS applications
Excellent batch-to-batch reproducibility for consistent results
Uniquely QC-tested specifically for peptide mapping
Consistent Results Due to Superior

Waters batch release protocol includes a tryptic map of cytochrome c which
is used to test for reproducibility to retention times and resolution. The
three test chromatograms below show the results of the protein digest test
for different batches of PA-B material.
Cytochrome c Tryptic Map Q.C. Test
BioSuite Peptide Analysis Series

BioSuite PA Series consists of two of Waters premier reversed-phase column
c hemistries specifically optimized for peptide mapping from simple to
complicated digests.
Eluent A:
Eluent B:
Flow rate:
Gradient:
Injection Volume:
Temperature:
Detection:
0.05% TFA in water

0.01% TFA in MeCN
1.0 ml/min
Time
Profile
(min)
%A
0.0
100
45
72
20 L
35 C
UV 220 nm
%B
0
28
BioSuite C18 3 m PA-A
Batch 112
BioSuite C18 3 m PA-A is a100, difunctional bonded, low ligand density,

silica-based column.
Batch 113
Batch 114
Specifically designed for excellent retention of polar peptides
Ideal choice for LC/MS applications using formic acid (FA) that
minimizes ion-suppression
Excellent performance for traditional HPLC separations using

low TFA concentrations (e.g., 0.025% TFA)
BioSuite C18 3.5 m PA-B

BioSuite C18 3.5 m PA-B is a 300, high ligand density, monofunctional,
silica-based column.
Outstanding performance when separating complex digests

containing hydrophilic, hydrophobic, and basic peptides
Superior peak shape and capacity for peptide separations

using TFA containing eluents (e.g., 0.1% TFA)
Good choice for the separation of larger peptide fragments

generated by some endoproteases (e.g., Lys-C)
212
10
15
20
25
30
35
40
45
50
55 Min.
Inner
Diameter
Length
BioSuite C18
3 m PA-A
BioSuite C18
3.5 m PA-B
2.1 mm
2.1 mm
2.1 mm
2.1 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
50 mm
100 mm
150 mm
250 mm
50 mm
100 mm
150 mm
250 mm
186002425
186002426
186002427
186002428
186002429
186002430
186002431
186002432
186002433
186002434
186002435
186002436
186002437
186002438
186002439
186002440
www.waters.com
BioSuite Cation-Exchange HPLC Columns

Separation of Angiotensins on BioSuite SP-PEEK
Cation-Exchange HPLC Column
BioSuite SP NP, SP-PEEK, and SP cation-exchange chemistries (CXC) consists

of the strong sulfopropyl ligand bonded to a pH stable (i.e., pH 212),
methacrylic ester-based polymeric resin. The availability of different pore
and particle size materials provides chromatographers with the flexibility
required to isolate and or characterize peptides based upon minor charge
differences. Non-porous (NP) and porous IEX columns are also available
to meet various separations requirements. Speed and superior chromatographic resolution are possible using the non-porous IEX offerings while
porous BioSuite offerings are available for applications requiring greater
peptide binding capacity. In addition, BioSuite SP material is offered in
PEEK hardware as well as in 21.5 mm i.d. stainless steel lab-scale
preparative column dimensions.
Column:
Eluent:
Flow rate:
Temperature:
Detection:
BioSuite SP-PEEK, 4.6 mm ID x 50 mm, PEEK

A: 20 mmol/L sodium acetate buffer, pH 5.0
B: 20 mmol/L sodium acetate buffer containing
[Val5]-II
1.0 mol/L NaCl, pH 5.0
[Sar1, IIe6]-II
Linear gradient from eluent
[Sar1, T hr8]-II
A to B for 20 minutes
[Sar1, Val5, Ala8]-II
1.0 mL/min.
[Val5]-II
25C
UV @ 280nm
III
II
BioSuite Q-PEEK and

SP-PEEK columns are available
in 4.6 x 50 mm
[Asn1, Val5]-II
Des-Asp1, [IIe8]-II
Exclusion Limit
(Daltons) against
Polyethylene
Inner
Glycol
Diameter
Pore
Size
Description
Matrix
BioSuite SP-PEEK 7 m CXC

BioSuite SP 2.5 m NP CXC
BioSuite SP 10 m CXC
Polymer 1300
Polymer
n/a
Polymer 1000
Polymer 1000
>4,000,000
500
1,000,000
1,000,000
4.6 mm
4.6 mm
7.5 mm
21.5 mm
10
15
20
25 Min.
Length
Column
Volume
(mL)
# Approx Protein
Binding Capacity
Per Pre-Packed
Column
Part No.
50 mm
35 mm
75 mm
150 mm
0.83
0.58
3.31
54.45
58 mg*
2.9 mg**
132 mg**
2,178 mg**
186002182
186002183
186002184
186002185
* = Data generated with Gamma Globulin ** = Data generated with Hemoglobin

# Note: For best resolution of complex samples, do not exceed 20% of the columns protein binding capacity
Delta-Pak HPLC Columns

Delta-Pak packings, ideal for the separation of peptides, proteins, and
natural products, are based on a highly stable, bonded, endcapped
5 m or 15 m spherical silica. Delta-Pak is available in two different
pore size materials (100 and 300) with a C18 or C4 bonded phase.
For more information and part numbers, visit waters.com/biosep.
Synthetic Peptide Separation on Delta-Pak C18 HPLC Column
Packing
Delta-Pak
Chemistry
Particle
Size
Particle
Shape
Pore
Size
Carbon
Load
Endcapped
C18
C18
5 m
5 m
Spherical
Spherical
100
300
17%
7%
Yes
Yes
C4
C4
5 m
5 m
Spherical
Spherical
100
300
7%
3%
Yes
Yes
C18
C18
15 m
15 m
Spherical
Spherical
100
300
17%
7%
Yes
Yes
C4
C4
15 m
15 m
Spherical
Spherical
100
300
7%
3%
Yes
Yes
Column:
Part Number:
Sample:
Injection:
Mobile Phase:
Gradient:
Conditions:
Flow Rate:
Detection:
Delta-Pak C18 5 m 300

3.9 x 150 mm
WAT011793
Synthetic peptide-neurotensin (5 mg/mL)
10 L (50 g)
Solvent A: H2O with 0.1% TFA
Solvent B: CH3CN with 0.1% TFA
0-2 min, 5% B
2-27 min, 5-9% B
30-31 min, 90-5% B
1 mL/min
230 nm
Waters Insulin HMW P HPLC Column

T he Waters Insulin HMW P column is specifically designed for use in the
manufacture and quality control of insulin products. T his column is tested
for performance in the analysis of impurities with molecular masses greater
than that of insulin.
Description
Waters Insulin HMWP Column
Protein-Pak 125 Sentry Guard Column,
2/pkg (requires holder)
Sentry Universal Guard Column Holder
Dimensions
Part No.
7.8 x 300 mm
WAT201549
3.9 x 20 mm
186000926
WAT046910
Tested to perform in the method published in PharmaEuropa Vol. 8, No 3, pages 359-360, September 1996.
www.waters.com
213
Symmetry HPLC Columns

Waters Symmetry, reversed-phase, silica-based particles are synthesized
using ultrapure organic reagents, resulting in high purity material with very
low silanol activity. When combined with the high surface coverage of the
bonded phase, outstanding peptide separations and recoveries are possible.
Superior manufacturing control for consistent

batch-to-batch and column-to-column results
100 and 300 pore size offerings for

small or larger size peptides
SymmetryShield column chemistry offers complementary

selectivity to Symmetry column offerings
SymmetryPrep columns provide direct scale up

while maintaining resolution
Symmetry300 Columns: T he First Columns Specifically Engineered

for the Discovery and Development of New Biopharmaceuticals
columns with minimal non-desired secondary interactions between

bound ligand and biomolecules.
There are several factors to assess when developing a reversed-phase
HPLC method for peptides. Batch-to-batch reproducibility of the column
is an important consideration when developing a validated and transferable method.Symmetry300 columns have outstanding batch-to-batch
and column-to-column reproducibility.
Pore Size Effects on Peptide Selectivity: Comparative Results
on Symmetry 100 vs. Symmetry300 Columns
Column:
Sample:
Mobile Phase:
High Recoveries of Peptides and Proteins

The heart of the column is high purity-based deactivated silica. Waters
dedicated chromatography chemistry manufacturing plant operates
under the stringent standards of cGMP supplemented with FDA registration for Class 1 medical devices and ISO 9002. The silica used in the
manufacture of our Symmetry300 columns is synthesized using ultrapure organic reagents that yields high purity particles with very low
silanol activity. These particles when combined with innovative ligand
(i.e., C4 and C18) bonding techniques helps produce reversed-phase
0-50 min, 0-30% B

20 L
0.75 mL/min
35 C
214 nm
13
56
23
12
8
11
In the past youve had no choice but to deal with variability by finding
costly and time-consuming ways around this problem. The regulatory guidelines being adopted worldwide as a result of the International Conference
on Harmonization (ICH) and the U.S. Food and Drug Administrations
specified biological products initiatives are placing increasingly stringent
demands on this process. Full analytical characterization is becoming
more important for regulatory filing, making column variability an
unacceptable risk.
Symmetry300 columns are a 300 reversed-phase addition to the

existing Symmetry family of columns. They have been specifically
designed to provide maximum batch-to-batch and column-to-column
performance consistency and recovery of protein and peptide applications. Symmetry300 columns are offered in two particle sizes (3.5 m
and 5 m) and in two chemistries (C4 for large peptides and proteins
and C18 for smaller peptides) to address various needs.
Gradient:
Injection:
Flow Rate:
Temperature:
Detection:
10
As a scientist, you know that tremendous time and resources are required
when developing HPLC assays for well-characterized biopharmaceuticals.
You also know that column-to-column variability is the Achilles heel of
this demanding process.
Now, regardless of whether youre developing HPLC assays for purity,

stability, or identity, you can have confidence in the long-term compliance of your methods, batch-to-batch, column-after-column, year-after-year.
Thats because Waters has developed Symmetry300 columns, specifically
engineered for the discovery and development of new biopharmaceuticals.
A. Symmetry C18, 5 m,
100, 4.6 x 150 mm
B. Symmetry300 C18, 5 m,
300, 4.6 x 150 mm
Tryptic digests of cytochrome c (bovine)
Solvent A: 0.1% TFA in water
Solvent B: 0.1% TFA in acetonitrile
10
13
5,6
B
2
3
4
7
12
20
11
40 Min.
The key to a successful separation is the selection of a column that gives

the highest chemistry resolution with maximum peak capacity and recovery.
Symmetry300 Columns
Inner
Diameter
Length
Particle
Size
C18
C4
1.0 mm
2.1 mm
2.1 mm
2.1 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
2.1 mm
3.9 mm
4.6 mm
4.6 mm
4.6 mm
19 mm
19 mm
19 mm
150 mm
50 mm
100 mm
150 mm
50 mm
75 mm
100 mm
150 mm
150 mm
150 mm
50 mm
150 mm
250 mm
10 mm
50 mm
100 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
186000185
186000187
186000188
186000200
186000201
186000189
186000190
186000197
WAT106172
WAT106154
WAT106209
WAT106157
WAT106151
186001847
186001848
186001849
186000276
186000277
186000278
186000279
186000280
186000281
186000282
186000283
186000285
186000286
186000287
186000288
186000289
For more complete listings, go to www.waters.com
214
www.waters.com
PROTEIN ISOLaTIONS aNd aNaLYSES

Protein Isolation and Characterization
Selecting the Separation Mode
Hydrophobic-Interaction Chromatography
T here are five principals of chromatography used for protein purification;

ion exc hange, size exclusion, reversed phase, affinity, and hydrophobic
interaction.
In this mode, proteins are bound to a moderately hydrophobic surface

at high ionic strength. T he proteins solubility is lowered under these
conditions. T he protein is then eluted with a gradient of decreasing ionic
strength. Hydrophobic-interaction chromatography often has good resolution and retains biological activity. Aqueous buffer systems are used in
hydrophobic interaction. Many different factors affect resolution, including buffer salt concentration and type, buffer pH, surfactant, modifiers,
column temperature, and packing material functionality. Since proteins
may also interact with one another under conditions of high ionic strength,
better resolution is achieved when the starting material is already partially
purified. It is also inconvenient to load large volumes of sample at high
ionic strength. T his would suggest that hydrophobic interaction would
normally be a good second or subsequent step in an isolation scheme, such
as when an ammonium sulfate precipitation occurs prior to the chromatographic protocol.
A protein purification protocol will always include several steps. T he

tec hniques should be used in a sequence that reflects different selectivity mec hanisms. T he order of steps should be c hosen to move from high
capacity and high speed to higher resolution.
Ion-Exchange Chromatography
In ion-exc hange separations, the distribution and net c harge on the
proteins surface determines the interaction of the protein with the
c harged groups on the packing materials surface. T he c harges on the
protein and the packing material must be opposite for interaction to
occur. T he variables that can be manipulated, suc h as buffer pH, buffer
composition, gradient slope, and gradient forming salt, offer a wide
range of options for optimizing a separation.
T he apparent size and shape of proteins in solution is the basis for
separation in size-exclusion c hromatography. T his simple separation mec hanism relies on protein size differences, packing material,
pore size, and particle size as well as the operating conditions, suc h
as sample concentration and volume for the successful resolution of
protein mixtures. Size-exclusion packing materials have evolved from
the large particle polysaccharide soft gels to small, rigid packings that
offer enhanced resolution and significantly faster separations.
Size exclusion has a wide range of applicability in protein isolations. It is
often used to assay fractions, as run times are relatively short, and the
technique relatively straightforward. It can also be used for buffer exchange,
as the low molecular weight salt molecules are easily separated from the
much larger proteins.
Reversed-Phase Chromatography
Reversed phase is an alternative hydrophobic-separation technique that
relies on the interaction between the proteins non-polar amino acid side
chains with the packing surface and ligands. T his mechanism provides
another high resolution dimension for protein chromatography that can
yield good recovery of biological activity with lower molecular weight
proteins and peptides. It is an excellent tool for assessing fraction purity
and for preparing proteins for sequencing or structural analysis.
Affinity Chromatography
Affinity is the only chromatographic mode in which proteins are isolated
on the basis of their biospecific interaction with an immobilized ligand.
Proteins can be recovered with retention of bioactivity and high purity.
Modern affinity packings take advantage of rigid base materials that
combine enhanced bed stability for crude sample processing with high
throughput for rapid microgram to gram scale affinity purifications. Many
separations can be achieved in a single step resulting in more costeffective separations.
www.waters.com
Particle Considerations for Lab-Scale Purifications

High resolution traditional HPLC packings for protein c hromatography
offer familiar c hemical functionalities bonded onto rigid, spherical
polymeric or silica supports. T hese packings provide improved c hemical and physical stability, better reproducibility and dramatically faster
separations than traditional soft gels. Columns containing lab-scale
preparative packings (8, 15, or 40 m) produce more concentrated
fractions. T he hydrophilic polymer with familiar functional groups result
in high recovery of biological activity.
Pore Size Considerations

Good peak shape and recovery for proteins had been observed on the
100-130 pore size materials; however, the bias is toward the 300
packings for protein samples. T he best advice is to test both 130 and
larger pore sizes (>300) as a step in the development of an optimized
protein separation. Purification and analysis media is available in pore
sizes ranging from 130 to 4000.
Sample Preparation
Protein samples derived from tissue homogenates, cell lysis, or physiologic
fluids frequently require removal of particulate contaminants prior to LC,
MS, or LC/MS analysis. In some situations, use of an on-line protein desalting device (e.g., MassPREP on-line desalting cartridge) can effectively be
used to remove ion-suppressing compounds prior to mass determination by
LC/MS techniques. Other situations exist where simple filtration or centrifugation can be effectively used to remove insoluble components prior to
analysis. Glycoproteins offer additional challenges that can be addressed
via the use of specialty reagents such as Waters RapiGest SF surfactant
that has been shown to significantly enhance protein deglycosylation. In
addition, solid-phase extraction techniques, such as those offered in Waters
MassPREP Deglycosylation Kit and Plate, can effectively isolate and concentrate glycans, cleaved from gylcoproteins, prior to analysis. Accell Plus
ion-exc hanger cartridges are also available for solid-phase extraction of
proteins from complex matrices or for isolation or sample concentration.
215

Strengths of Different Modes of Chromatography
Ion Exchange
Hydrophobic Interaction
Affinity
Reversed Phase
Size Exclusion
Concentrates sample
Concentrates sample
Concentrates sample
Concentrates sample
Rapid
Column can be cleaned

using NaOH
Column can be cleaned

using NaOH
Superb selectivity
Good resolution for

proteins and peptides
Good recovery of activity
Moderate to high resolution
Moderate resolution
High capacity
High capacity
Widespread applicability
Outstanding resolution
High capacity
Simple technique
Reliable medium to high

resolution technique
Moderate capacity
Can be used to buffer exchange

Compatible with detergents
Significant purification
in a single step
Limitations of Different Modes of Chromatography

Ion Exchange
Hydrophobic Interaction
Affinity
Reversed Phase
Size Exclusion
Fractions may need to be

desalted prior to the next
purification step
Fractions may need to be

desalted prior to the next
purification step
Ligand leakage
Limited applicability with

high MW proteins
Moderate resolution
Column cannot be cleaned

using aggressive chemicals
Stationary phases can
be expensive
Column cannot be cleaned

using aggressive chemicals
Non concentrating
Moderate capacity
Organic mobile phase

may denature protein
Planning a Protein Isolation Protocol
Typical Isolation Protocol
T he design of an overall c hromatography protocol depends on many

factors, such as the sample volume, the cleanliness of the starting material,
whether economic affinity supports are available, and what the final use of
the purified protein might be.
Taking all these facts into consideration, a typical purification protocol

would start with an ion-exchange step, as the resolving power is good where
a dilute feed stream can be concentrated prior to elution and the packing
materials can be cleaned using aggressive agents. As the protein is usually
eluted using an increasing salt gradient, the active fractions often contain
high concentrations of salt. It is sometimes necessary to desalt the fractions
prior to the next step. T his can be avoided if the next step is hydrophobic
interaction, where the protein is loaded onto the column in a concentrated
salt solution. In some instances, especially where the feedstream comes
from an ammonium sulfate precipitation, hydrophobic interaction may be
a good first purification step, followed by ion exchange.
For instance, many of the initial feed streams for protein isolations are
relatively dilute protein solutions. Chromatographic modes that concentrate
samples (ion exchange, hydrophobic interaction, and affinity) are good starting points for these solutions.
For samples such as cell lysates and homogenates or physiologic fluids,
there are additional considerations. T hese mixtures contain cell fragments,
lipids, and nucleic acid fragments, which will bind strongly to a column.
Columns becoming contaminated in this way are typically cleaned using
0.1 M -1.0 M NaOH prior to re-equilibration with the starting buffers.
T his would destroy a peptide or protein ligand on an affinity column
and would irreversibly damage a silica-based, reversed-phase column.
T herefore, ion exchange or hydrophobic interaction would be appropriate
modes for a first purification step.
In reversed-phase chromatography, the sample is loaded onto the column
in an aqueous environment and then eluted using an increasing gradient of
an organic solvent, such as acetonitrile or propanol. T he major drawback to
this mode of chromatography is that some proteins will denature (causing
loss of biological activity) in the presence of the organic solvents at the
concentration required to elute the protein. As a general rule, the likelihood
of denaturation increases with increasing molecular weight of the protein.
216
T he eluate from this ion-exc hange and/or hydrophobic-interaction step

is usually free of lipids and other contaminants that may cause problems
with an affinity column. It can now be put onto an affinity column
should one be available. T he use of an affinity column is possible, if
available, once lipids and other contaminants have been removed by
the ion-exc hange and/or hydrophobic-interaction step. Alternatively,
reversed-phase c hromatography or a second ion-exc hange step under
different conditions or with higher resolving power (smaller particle size
or longer column) can be used. Both of these approac hes result in high
resolution and sample concentration.
T he final step in the purification is often size exclusion, which will separate
out dimers and polymers of the protein of interest, and potentially desalt
the protein.
www.waters.com
ACQUITY UPLC SEC System Solution

Introducing the ACQUITY UPLC
BEH200 SEC, 1.7 m Column
ACQUIT Y UPLC tec hnology allows analytical c hemists to reac h far
beyond conventional LC separations and has proven itself to be a major
asset in increasing the productivity of laboratories around the world. T he
latest addition to this application-driven portfolio is the ACQUITY UPLC
SEC System Solution, enabled by the unique Ethylene Bridged Hybrid
(BEH) Diol coated 1.7 m particle technology.
Determines aggregation levels in t herapeutic monoclonal

antibodies up to 10 times faster t han traditional HPLC
based Size Exclusion C hromatography (SEC)
Fully optimized column chemistry significantly reduces the

requirement for high salt concentration mobile phases
QC tested with relevant proteins, ensuring unmatched

batch-to-batch consistency and increased confidence
in validated QC release methods
BEH Technology
T he BEH Particle*: One of the Key Enablers of UPLC Technology
In 1999, Waters launched the XTerra family of HPLC columns featuring

patented-first-generation Hybrid-Particle Technology (HPT)*. HPT enabled
XTerra columns to become one of the most successful column products in
the history of Waters. In HPT, the best properties of inorganic (silica) and
organic (polymeric) packings are combined to produce a material that has
superior mechanical strength, efficiency, high-pH stability and peak shape
T he first-generation methyl-hybrid particles of XTerra columns did not
possess the mechanical strength or efficiency necessary to fully realize
the potential speed, sensitivity and resolution capabilities of UPLC
Tec hnology. T herefore, a new pressure-tolerant particle needed to be
created. T his new, second-generation hybrid material was developed that
utilizes an ethylene-bridged hybrid (BEH) structure. Compared to the firstgeneration methyl-hybrid particle of XTerra columns, the BEH particle of
ACQUITY UPLC BEH columns exhibits improved efficiency, strength and
pH range. BEH Technology is a key enabler of the speed, sensitivity and
resolution of both small and large molecule UPLC separations.
E tO C H 2 C H 2 O E t
Si O
Si
O
O
Si
E tO
OE t
Si O
Si
Et
O
Si
O Et
O Et
OEt
E tO
E tO
Si
E tO
O Et
E tO
E tO S i
E tO
Polyethoxysilane
(BPEOS)
Tetraethoxysilane
(TEOS)
Bis(triethoxysilyl)ethane
(BTEE)
Anal. Chem. 2003, 75, 6781-6788
www.waters.com
O Et
CH 2
Si O Et
CH 2
O Et
*Patent No. 6,686,035 B2
217

Fast and Accurate mAb Aggregate Determination
from 10,000 to 450,000 Daltons, the total system solution will routinely
deliver fast, accurate aggregate determinations up to 10 times quicker that
traditional HPLC-based SEC methods.
Based upon the highly successful BEH particle technology, the new patent
pending ACQUIT Y UPLC BEH200 SEC column represents the first
commercially available sub 2 m SEC chemistry. Fully optimized for the
analysis of proteins and their aggregates with molecular weights ranging
Traditional HPLC SEC Chromatography
ACQUITY UPLC SEC System Solution
mAb monomer
mAb monomer
Aggregates
10.3%
Aggregates
10.7%
Column:
Sample:
HPLC system:
Eluent:
Flow Rate:
Injection:
Wavelength:
10
15
20
25
30
35
40 Min.
Column:
Traditional 100% Silica-based / Diol coated,

5 m, 7.8 x 300 mm SEC column
Humanized IgG monoclonal Antibody,
3 mg/mL in eluent
Waters Model 2796 HPLC with Model 2487
Dual Absorbance Detector
25 mM sodium phosphate, pH 6.8
with 0.15 m sodium chloride
0.4 mL /min
20 L
280 nm
6 Min.
ACQUIT Y UPLC BEH200 SEC, 1.7 m,

4.6 x 150 mm
Humanized IgG monoclonal Antibody,
3 mg/mL in eluent
Waters ACQUIT Y UPLC System with
Tunable UV Detector
25 mM sodium phosphate, pH 6.8
0.4 mL /min
5 L
280 nm
Sample:
UPLC system:
Eluent:
Flow Rate:
Injection:
Wavelength:
Protein Calibration Curve for ACQUITY UPLC BEH200 SEC,

1.7 m, 4.6 x 150 mm Column
10000000
1000000
IgG (150,000 Da)
MW
100000
Ribonuclease A
(~13,700 Da)
10000
1000
100
10
1
0
0.5
1.5
2.5
Elution Volume
218
www.waters.com
PROTEIN IsOlaTIONs aNd aNalysEs

Reduced Requirement for High Salt Concentration Mobile Phases
to compete for the charged sites on the surface of the silica. T he unique
surface chemistry of the ACQUITY UPLC BEH200 SEC column significantly
reduces these secondary interactions, resulting in the ability to use less
aggressive mobile phase salt concentrations.
With conventional silica based SEC column c hemistries, undesirable

secondary ionic interactions between the silica surface and basic proteins
can result in long retention times and excessive peak tailing. Traditionally,
the solution to this issue is the inclusion of high concentrations of a salt
Effect of Eluent Ionic Strength on the SEC Analysis of the Basic Protein Lysozyme on 100% Silica vs BEH SEC Particles
Flow rate:
Mobile phase:
0.3 mL/min
10, 25 or 100 mM
sodium phosphate, pH 6.8
Traditional 100% Silica-based / Diol coated, 4 m,

4.6 x 300 mm SEC column
Mobile phase Ionic Strength
10 mM 25 mM 100 mM
Lysozyme, 10 mM
10
14
18
22
26
30
34
38
42
50 Min.
46
ACQUITY UPLC BEH200 SEC, 1.7 m, 4.6 x 150 mm

Significant reduction in 2 interactions
10
11
12
13
15 Min.
14
A New Level of Column Stability for Size Exclusion Chromatography

T he Ethylene Bridged Hybrid (BEH) particle technology is well established
for chromatography of synthetic oligonucleotides, amino acids, peptides,
proteins, and labeled glycans with stability and performance attributes
not found with traditional, 100% silica-based particles. (go to
www.waters.com/biosep)
T he combination of the BEH base particle and the patent pending innovative
diol bonding process, results in column stability, performance and lifetime
unheard of in traditional size exclusion chromatographic columns.
ACQUITY UPLC BEH200 SEC Particle and DIOL Bonding Technology Provides a Stable Chemistry with Outstanding Column Life
Sample:
Flow rate:
Mobile phase:
Lysozyme
0.4 mL/min
25 mM sodium
phosphate, pH 6.8
Traditional 100% Silica-based / Diol coated,

4 m, 4.6 x 300 mm SEC column
Injection 19
Injection 618
10
11
12
13
14
15 Min.
ACQUITY UPLC BEH200 SEC,

1.7 m, 4.6 x 150 mm
Injection 19
Injection 618
0
www.waters.com
10 Min.
219

Stringent Manufacturing Quality Assurance Delivers Confidence in Results
All Waters ACQUITY columns chemistries are synthsised in state-of-the-art
ISO certified manufacturing facilities from high quality raw materials, and
are extensively QC tested throughout the synthetic process. In addition,
eac h batc h of BEH200 SEC 1.7 m material is specifically tested with
relevant proteins (see table below) to help ensure unmatched batch-to-batch
consistency for supreme confidence in validated methods.
Waters ISO 2001 Manufacturing and Testing Processes

Help Ensure Outstanding ACQUITY UPLC BEH200, SEC,
1.7 m Batch-to-Batch Reproducibility
4
Batch A
Analyte
pI
MW
1. Thyroglobulin, 3 mg/mL (Sigma P/N T1001)
4.6
669,000
2. IgG, 2 mg/mL
6.7
150,000
3. BSA, 5 mg/mL (Sigma P/N A8022)
4.6
66,400
6.8, 7.2
17,000
N/A
112
4. Myoglobin, 2 mg/mL (Sigma P/N M1882)

5. Uracil, 0.1 mg/mL (Sigma P/N U0750)
1
5
10 Min.
Batch B
2
3
1
5
10 Min.
Batch C
2
3
Description
ACQUITY UPLC BEH200, 1.7 m SEC
ACQUITY UPLC BEH200, 1.7 m SEC
Dimensions
Part No.
4.6 x 150 mm
4.6 x 300 mm
186005225
186005226
10 Min.
For Licensed use only on a Waters ACQUITY UPLC System

Notes: Size-exclusion c hromatography may require modifications to
an existing ACQUIT Y UPLC system. Please refer to Size Exclusion and
Ion- Exc hange Chromatography of Proteins using the ACQUIT Y UPLC
System, (715002147A) for specific recommendations that can be
obtained at www.waters.com/c hemcu.
Literature
References
Protein Separation Technology Brochure,
Protein-Pak Hi Res Ion Exchange
(IEX) Columns for ACQUITY UPLC
Applications Solution Brochure,
ACQUITY UPLC SEC System Solution Brochure,
220
www.waters.com
Protein Separation Technology

T he analysis and characterization of protein samples requires the detection of small c hemical differences between large molecules. Most often
these analyses have employed an array of analytical tec hniques, eac h
sensitive to a different property of the protein. Reversed-phase HPLC has
not been fully exploited in these tests because the separation of proteins
often yields relatively broad and asymmetrical peaks with poor recovery
and significant carryover. Waters new reversed-phase, ethylene-bridged
hybrid (BEH Tec hnology) Protein Separation Tec hnology columns are
specifically designed for the high resolution analysis of proteins.
Waters New Family of BEH300 C4 columns for protein separations:
Separates proteins of various sizes,

hydrophobicities, and isoelectric points
Available as 3.5 m packing

for HPLC and 1.7 m packing
for UPLC methods
Maximizes recovery and

minimizes protein carryover
Tolerates extreme pH
and temperature
Quality-control tested with

protein mixture
Couples directly to ESI-MS

for protein identification
300 C4 Columns Developed for Protein Chromatography

Column:
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
Column Temp.:
Injection:
Detection :
XBridge BEH300 C4, 3.5 m,

2.1 x 50 mm
0.1% TFA in water
0.075% TFA in 71.4% acetonitrile
0.2 mL/min
28-100% B in 25 min
40 C
5 L
220 nm
Sample:
MassPREP Protein Std (Part No. 186004900)
0.1% TFA in 5% acetonitrile
1. Ribonuclease A (0.04 mg/mL)
2. Cytochrome c (0.06 mg/mL)
3. Bovine Serum Albumin (0.20 mg/mL)
4. Myoglobin (0.13 mg/mL)
5. Enolase (0.22 mg/mL)
6. Phosphorylase b (0.59 mg/mL)
Column:
ACQUITY UPLC BEH300 C4, 1.7 m,

2.1 mm x 50 mm
0.1% TFA in 100% HPLC grade water
0.2 mL/min
28-100% B in 25 min
40 C
220 nm
Eluent A:
Eluent B:
Flow Rate:
Gradient:
Column Temp.:
Detection :
Sample:
MassPREP Protein Std (Part No. 186004900)
1. Ribonuclease
2. Cytochrome c
3. BSA
4. -Lactoglobulin
5. Enolase
6. Phosphorylase b
5
4
0.15
Batch A
0.10
AU
3
6
Batch B
0.05
Batch C
0.00
12
18 min
BEH300 C4 columns can be used with proteins that have a wide range of properties. This protein mix was
chosen to represent a range of isoelectric points, molecular weights, and hydrophobicities.
www.waters.com
10
12
14
16
18
20
22 Min.
This comparison shows the consistent batch-to-batch performance with a protein separation.
221

Minimal Protein Carryover
Absence of detectable Ovalbumin carryover
Ovalbumin
0.05
Sample:
Columns:
1.3 g Ovalbumin injected

ACQUITY UPLC BEH300 C4, 1.7 m,
2.1 mm x 50 mm
0.1% TFA in 100% HPLC grade water
0.2 mL/min
28-100% B in 25 min
Hold at 100 %B for 2 min then
re-equilibrate column at 28% B for 18 min
Gradient restarted at 45 min and
at 90 min
40 C
220 nm
AU
Eluent A:
Eluent B:
Flow Rate:
Gradient:
0.00
0
10
20
30
40
50
60
70
80
90
100
1st gradient without

sample injection
110
120
Column Temp.:
Detection :
130 min
2nd gradient without

sample injection
Column carryover was tested by running multiple gradients following a single injection. Protein peaks observed during the first gradient are not found in subsequent gradients.
Improved Protein Resolution with UPLC Technology

Column:
ACQUITY UPLC BEH300 C4,

2.1 150 mm, 1.7 m (top), 3.5 m (bottom)
0.1% TFA in water
0.2 mL/min
20-55% B in 60 min
80 C
3.3 L
220 nm
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
Column Temp.:
Injection:
Detection :
AU
Sample: MassPREP Protein Standard

(Part No. 186004900)
1. Ribonuclease A (0.04 mg/mL)
2. Cytochrome c (0.06 mg/mL)
3. Bovine Serum Albumin (0.20 mg/mL)
4. Myoglobin (0.13 mg/mL)
5. Enolase (0.22 mg/mL)
6. Phosphorylase b (0.59 mg/mL)
0.24
4
2
Waters LCT Premier XE MS Detection

Capillary Voltage:
3200 V
Cone Voltage:
45 V
Source Temp.:
150 C
Desolvation Temp.:
350 C
Desolvation Gas:
000 L/h
Lys-C digested (limited)

reduced monoclonal antibody
ACQUITY UPLC BEH300 C4,
1.7 m, 2.1 x 50 mm
0.1% FA in 100% HPLC grade water
0.1% FA in 100% acetonitrile
0.2 mL/min
5% B for 5 min
Then 5-21% B in 0.1 min.
Then 21-29% B in 30 min.
80 C
Column:
Eluent A:
Eluent B:
Flow Rate:
Gradient:
Fd
LC
Fc/2
6
%
0.12
Sample:
Column Temp.:
BEH300 C4
3.5 m
0.36
BEH300 C4 Columns for Protein Characterization with UPLC / MS
0.00
AU
0.36
BEH300 C4
1.7 m
0.24
Fc/2
Clip 2
1
0.12
0.00
Fc/2
Clip 1
12
24
36
48
60 min
The protein test mixture was separated on two BEH300 C4 columns, one with 3.5 m particles and the other with
1.7 m particles. The UPLC separation provides sharper peaks for all the proteins in the test mixture. This translates
into better resolution as shown by the multiple peaks around phosphorylase, at approximately 50 min. This comparison, with both columns, was performed on a UPLC system to preserve the minimized band-broadening. The benefits of
the small particle UPLC BEH300 C4 column would be lost without the optimized ACQUITY UPLC System.
10
12
14
16
18
20
22
24
26
28
30
32
34 Min
T he large fragments obtained through LysC digestion of a monoclonal antibody can be separated on the UPLC
BEH300 C4 column coupled directly to ESI/Tof MS for identification of the individual peptide products.
Waters BEH300 C4 Offerings for Traditional HPLC and Advanced UPLC Protein Separations
UPLC Columns
Particle Size
Dimensions
Part No.

1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
1 x 50 mm
1 x 100 mm
1 x 150 mm
186004495
186004496
186004497
186005589
186005590
186005591
1.7 m
VanGuard Pre-Column
186004623
Note: ACQUIT Y UPLC BEH300 C4, 1.7 m columns are designed for
use with the ACQUITY UPLC system. T he benefits of the small particle
packing in ACQUITY UPLC BEH300 C4, 1.7 m columns are only realized
with the low system volume and low detector dispersion of an ACQUITY
UPLC system.
222
HPLC Columns
XBridge BEH300 C4
XBridge BEH300 C4
XBridge BEH300 C4
XBridge BEH300 C4
XBridge BEH300 C4
XBridge BEH300 C4
XBridge BEH300 C4
XBridge BEH300 C4
Custom BEH300 C4
ACQUITY BEH300 C4
Method Validation Kit*
Particle Size
Dimensions
Part No.
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
2.1 x 50 mm
2.1 x 100 mm
2.1 x 150 mm
2.1 x 250 mm
4.6 x 50 mm
4.6 x 100 mm
4.6 x 150 mm
4.6 x 250 mm
186004498
186004499
186004500
186004501
186004502
186004503
186004504
186004505
186004506
1.7 m
2.1 x 100 mm
186004899
1.7 m
1.7 m
1.7 m
1.0 x 50 mm
1.0 x 100 mm
1.0 x 150 mm
186005589
186005590
186005591
*Three columns from three different batches of material.
www.waters.com
Protein-Pak Hi Res Ion-Exchange (IEX) columns

For ACQUITY UPLC Applications
Protein-Pak Hi Res Ion-Exchange (IEX) columns were developed to assist in
the characterization of recombinant proteins, monoclonal antibodies, and
other biological compounds. T he non-porous, high compound binding
capacity of these particles yields outstanding resolution of charged species
in less time compared to use of many traditional porous IEX offerings. In
addition, quality control testing with defined protein standards helps ensure
consistent batch-to-batch performance.
Designed for the characterization of protein charge variants

and other biocompounds.
Two cation-exchangers (Carboxymethyl and Sulfopropyl)

and one anion-exchanger (Quaternary ammonium) that
address selectivity needs.
Non-porous, high-capacity stationary phases deliver fast

separations that address high-through put needs.
QC-Tested with protein standards to ensure batch-to-
columns
batch consistency.
eCord-enabled to help monitor column use on

ACQUITY UPLC Systems.
Resolved Monoclonal Antibody (mAb) Product

from Variants in Less Time
Protein-Pak Hi Res CM Analysis of T hree mAbs

Containing Different Levels of Variants
Column:
Waters Protein-Pak Hi Res CM, 7 m,

4.6 x 100 mm Column
0.67 mL/min
20 mM Na2HPO4 pH 7
0.7 M NaCl in B
0-10% B in 25 min
30 C
280 nm
Flow Rate:
Eluent A:
Eluent B:
Gradient:
Temperature:
Detection:
0.040
0.030
0.020
0.010
ACQUITY UPLC System configuration guidelines for ion-exchange

are described in service notes P/N 715002147A.
Column:
Flow Rate:
Mobile Phase A:
Mobile Phase B:
Gradient:
Temperature:
Detection:
ProteinPak Hi Res CM, 7 m, 4.6 x 100 mm

0.67 mL/min
20 mM Sodium Phosphate Buffer, pH 7.0
20 mM Sodium Phosphate Buffer, 0.7 M NaCl, pH 7.0
010% B in 25 min
30 C
280 nm
0.000
0.040
0.030
Column:
Competitors Weak-Cation Exchanger,

4 x 250 mm Column
0.51 mL/min
20 mM Na2HPO4 pH 7
0.7 M NaCl in B
0-10% B in 62.5 min
Flow Rate:
Eluent A:
Eluent B:
Gradient:
0.020
Humanized IgG1
0.010
0.000
0.020
0.008
Humanized IgG2
0.010
0.006
0.000
0.004
0.015
0.002
0.000
K1
K0
0.010
K2
Chimeric IgG1
0.005
10
12
14
16
18
20
22
24
26
28
30 min
0.000
Cation-Exchange chromatography is a useful tool for the characterization and quantitation of mAb or
recombinant protein variants. Use of Waters Protein-Pak Hi Res CM column on an ACQUITY UPLC System
increases sample throughput while maintaining resolution between intended product and undesired variants.
www.waters.com
10
12
14
16
18
20
22
24 min
Sequence, production, storage, and shipping conditions influence the degree of variants contained in a biotherapeutic
protein. Waters Protein-Pak Hi Res CM column can successfully resolve variations that may involve as little as a
single amino acid change (K0 = No terminal lysines, K1 = One terminal Lysine, and K2 = Two terminal Lysines).
223

Application of Waters UPLC Technology for
Biotherapeutic Characterization
Manufacturing Consistency for Enhanced Assurance
ACQUITY UPLC allows analytical chemists to reach far beyond conventional

LC separations and has proven itself to be an asset to laboratories around
the world. UPLC sets new standards in resolution, sensitivity, and throughput by being the first holistically-designed system that maximizes for rapid,
high-resolution analyses. It has fueled hundreds of peer-reviewed papers,
helps laboratories conserve resources, and has served the needs of
regulatory agencies around the globe. ACQUITY UPLC simultaneously
makes your laboratory more sustainable and more efficient.
ACQUIT Y UPLC Tec hnology
for biotherapeutic
c haracterization.
T he ability to obtain the same high quality separations regardless of column

lot is of critical importance to the successful development and commercialization of biotherapeutics. Each batch of Protein-Pak Hi Res IEX material is
tested with a relevant mixture of protein standards to help ensure consistent
column-to-column performance.
Protein-Pak Hi Res IEX column Batch to Batch Reproducibility
Column:
Protein-Pak Hi Res Q, 5 m,
4.6 x 100 mm
0.6 mL/min
20 mM Tris Buffer, pH 8.5
20 mM Tris Buffer, pH 8.5, 0.5 M NaCl
0-60% B in 30 min
30 C
280 nm
Flow Rate:
Mobile Phase A:
Mobile Phase B:
Gradient:
Temperature:
Detection:
0.02
0.01
Batch A
0.00
0.02
0.01
Myoglobin
Apo-Transferrin
Batch B
Novel IEX Particles Ideal for Biomolecule Characterizations

0.00
Protein-Pak Hi Res IEX columns contain non-porous, pH tolerant, hydrophilic

particles whose surface consists of a multi-layered network or either anion
(5 m) or cation (7 m) exchange groups. T his innovative particle and
bonding chemistry produces particles with greater protein loading capacities than found on many traditional mono-disperse, non-porous resins. T his
translates into columns that can resolve complex mixtures of biomolecules
in comparatively short times compared to use of alternative porous or
non-porous IEX column offerings.
Description
Protein-Pak
Hi Res Q
Protein-Pak
Hi Res CM
Protein-Pak
Hi Res SP
Ion Exchange
Strong Anion
Weak Cation
Strong Cation
Quaternary
ammonium
Carboxymethyl
Sulfopropyl
Hydrophilic polymer
Hydrophilic polymer
Hydrophilic polymer
Pore size
Non porous
Non porous
Non porous
i.d. x L (mm)
4.6 x 100
4.6 x 100
4.6 x 100
Counter ion
Cl-
Na +
Na +
Functional Group
Matrix
Particle size (m)
pH range
Temperature (C)
pKa
Flow Rates
3-10
3-10
3-10
10-60
10-60
10-60
10.5
4.9
2.3
0.3-0.6 mL/min
0.5-1.4 mL/min
0.5-1.4 mL/min
58
33
25
0.02
Trypsin Inhibitor
0.01
Batch C
0.00
3
10
11
12
13 min
Each batch of Protein-Pak Hi Res SP, CM, and Q column packing material is chromatography tested using a relevant
protein standard mixture to help ensureconsistent and predictable performance (Protein-Pak Hi Res Q data shown).
Description
Protein-Pak Hi Res CM, 7 m
Protein-Pak Hi Res SP, 7 m
Protein-Pak Hi Res Q, 5 m
Dimensions
Part No.
4.6 x 100 mm
4.6 x 100 mm
4.6 x 100 mm
186004929
186004930
186004931
Note: Only when Protein-Pak Hi Res IEX columns are combined with the
ACQUIT Y UPLC system are the full performance benefits realized. See
Waters service notes P/N 715002147A for ACQUIT Y UPLC system
configuration guidelines for ion-exchange chromatography.
More information regarding this and additional columns for protein sample
preparation and analysis can be found at www.waters.com/Proteins.
Approx Protein Binding Capacity in mgs

per column (i.e., BSA
for Hi Res Q Column.
Lysozyme for Hi Res
CM and Hi Res SP
Columns)
For optimal resolution of complex samples, do not exceed 20% of the columns protein binding capacity.
224
www.waters.com
QC-Tested Specifically for Bio-Applications

Waters is a leading manufacturer of chemistry consumables for the
accurate analysis of amino acids, synt hetic oligonucleotides,
peptides, proteins, and glycans.
From nano to preparative scale, our columns are quality-control
tested with relevant biological compounds to ensure consistent
performance and high-quality results.
www.waters.com/biosep
2010 Waters Corporation. Waters and T he Science of W hats Possible

are trademarks of Waters Corporation.

BioSuite Ion-Exchange HPLC Columns
BioSuite ion-exchange column offerings include strong and weak, cation(CXC) and anion exchangers (AXC) bonded to a pH stable (i.e., pH 2-12),
methacrylic ester-based polymeric resin. T he availability of four separation chemistries provides chromatographers with the flexibility required
to develop methods that separate proteins or peptides based upon minor
c harge differences. Non-porous (NP) and porous IEX columns are also
available. Speed and superior chromatographic resolution are possible

using the NP IEX offerings. Waters porous ion exchangers are available
for applications requiring greater protein or peptide binding capacity.
In addition, selected BioSuite ion-exchange columns are offered in PEEK
hardware as well as in 21.5 mm i.d. preparative column sizes.
Protein Selectivity Differences on BioSuite CM (Weak CationExchange) vs. SP (Strong Cation-Exchange) Columns
Enhanced Compound Resolution by Ion-Exchange Chromatography

on BioSuite SP Non-Porous (NP) vs. Porous CXC Columns
A) BioSuite CM,
10 m CXC
A) BioSuite SP,
2.5 m NP CXC
2
4
B) BioSuite SP,
10 m CXC
1
B) BioSuite SP,
10 m CXC
3
1
0
Sample:
Columns:
Eluent: A:
Eluent B:
Flow Rate:
Gradient:
Temperature:
Detection:
10
5
2
20
30
40 Min.
Trypsinogen (Peak 1), Ribonuclease (Peak 2),

Alpha-Chymotrypsinogen A (Peak 3),
Cytochrome c (Peak 4), and Lysozyme (Peak 5)
A: BioSuite CM, 10 m CXC (7.5 x 75 mm)
B: BioSuite SP, 10 m CXC (7.5 x 75 mm)
20 mM Sodium Phosphate, pH 7.0
20 mM Sodium Phosphate, pH 7.0 containing 0.5 M NaCl
1.0 mL/min
Linear gradient from 0 to 100% B in 60 min.
25 C
280 nm
Description
BioSuite Q-PEEK 10 m AXC
BioSuite SP-PEEK 7 m CXC
BioSuite Q-PEEK and

SP-PEEK columns are available
in 4.6 x 50 mm
4 3
Sample:
Columns:
Eluent: A:
Eluent B:
Flow Rate:
Gradient:
Temperature:
Detection:
Matrix
Pore
Size
Polymer 4000
Polymer 1300
BioSuite DEAE 2.5 m NP AXC Polymer

BioSuite SP 2.5 m NP CXC
Polymer
5 4
6 Min.5
6 20
Min.
20 30
30
40 Min.
40 Min.
Alpha-Chymotrypsinogen A (Peak 1) and Ribonuclease (Peak 2)

A: BioSuite SP, 2.5 m NP CXC (4.6 x 35 mm)
B: BioSuite SP, 10 m CXC (7.5 x 75 mm)
20 mM MES [2[N-morpholino]ethanesulfonic acid], pH 6.0
20 mM MES, pH 6.0 containing 0.5 M NaCl
A: 1.5 mL/min
B: 1.0 mL/min
A: Linear gradient from 0 to 100% B in 10 min.
B: Linear gradient from 0 to 100% B in 60 min.
25 C
280 nm
Exclusion Limit
(Daltons) against
Polyethylene
Inner
Glycol
Diameter
Length
# Approx Protein
Column Binding Capacity
Volume Per Pre-Packed
(mL)
Column
Part No.
>5,000,000
>4,000,000
4.6 mm
4.6 mm
50 mm
50 mm
0.83
0.83
58 mg*
58 mg**
186002176
186002182
n/a
n/a
500
500
4.6 mm
4.6 mm
35 mm
35 mm
0.58
0.58
2.9 mg*
2.9 mg***
186002179
186002183
BioSuite Q 10 m AXC
BioSuite Q 13 m AXC
BioSuite DEAE 10 m AXC
BioSuite DEAE 13 m AXC
Polymer
Polymer
Polymer
Polymer
1000
1000
1000
1000
1,000,000
1,000,000
1,000,000
1,000,000
7.5 mm
21.5 mm
7.5 mm
21.5 mm
75 mm
150 mm
75 mm
150 mm
3.31
54.45
3.31
54.45
331 mg*
5,445 mg*
99 mg*
1633 mg*
186002177
186002178
186002180
186002181
BioSuite CM 10 m CXC
BioSuite CM 13 m CXC
Polymer
Polymer
Polymer
Polymer
1000
1000
1000
1000
1,000,000
1,000,000
1,000,000
1,000,000
7.5 mm
21.5 mm
7.5 mm
21.5 mm
75 mm
150 mm
75 mm
150 mm
3.31
54.45
3.31
54.45
132 mg***
2,178 mg***
149 mg***
2,450 mg***
186002184
186002185
186002186
186002187
* Data generated with BSA ** Data generated with Gamma Globulin *** Data generated with Hemoglobin
# Note: For best resolution of complex samples, do not exceed 20% of the columns protein binding capacity
226
www.waters.com

Protein-Pak HR Ion-Exchange Glass Columns
T he Protein-Pak HR 8 m and 15 m packing materials are available
prepacked in Waters Advanced Purification (AP) glass columns in a choice of
5 mm i.d. (minicolumn) or 10 mm i.d. by 100 mm in length. T he 5 mm i.d.
column is also available in a 50 mm length. T hese columns are compatible
with any HPLC and FPLC system with the use of an adapter kit.
Waters Protein-Pak HR packing materials are based on rigid, hydrophilic,
polymethacrylate particles with large 1000 pores. T he naturally hydrophilic polymer reduces non-specific adsorption, resulting in quantitative
recovery of protein mass and bioactivity. T hese packings are compatible
with buffers in the pH range 2-12, and will withstand exposure to caustic
Ion-Exchange
Packing
Particle
Type
Pore
Size
Size
Dimensions
Part No.
Protein-Pak
Q 8HR
Polymeric strong
anion exchanger
8 m
1000
5 x 50 mm
5 x 100 mm
10 x 100 mm
5 x 50 mm
10 x 100 mm
5 x 50 mm
5 x 100 mm
10 x 100 mm
5 x 50 mm
5 x 100 mm
10 x 100 mm
5 x 50 mm
5 x 100 mm
10 x 100 mm
10 x 100 mm
WAT039575
WAT039630
WAT035980
WAT039782
WAT037663
WAT039791
WAT039783
WAT035650
WAT039780
WAT039786
WAT038564
WAT039570
WAT039625
WAT035655
WAT038567
5 x 50 mm
5 x 100 mm
10 x 100 mm
5 x 50 mm
WAT039790
WAT039785
WAT035970
WAT039787
Protein-Pak
Q 15HR
Protein-Pak
DEAE 8HR
Polymeric strong
anion exchanger
Polymeric weak
anion exchanger
15 m
1000
8 m
1000
Protein-Pak
DEAE 15HR
Polymeric weak
anion exchanger
15 m
1000
Protein-Pak
SP 8HR
Polymeric strong
cation exchanger
8 m
1000
Protein-Pak
SP 15HR
Protein-Pak
CM 8HR
Polymeric strong
cation exchanger
Polymeric weak
cation exchanger
15 m
1000
8 m
1000
Protein-Pak
CM 15HR
Polymeric weak
cation exchanger
15 m
1000
solutions, such as 0.1-1.0 M sodium hydroxide and acetic solutions, such as

20% acetic acid, for cleaning purposes.
Protein-Pak HR ion exc hangers are available with a Q functional group,
a strong anion exc hanger; DEAE, a weak anion exc hanger; SP a strong
cation exchanger; and CM, a weak cation exchanger. T he principle difference between a weak and strong ion exchanger does not lie in the protein
binding capacity, but in the pH range of operation. Weak ion exchangers
tend to have a more restricted useful pH range of operation.
Protein Resolution on Protein-Pak DEAE 15HR

Anion-Exchange Column
Column:
Buffer A:
Buffer B:
Flow Rate:
Gradient:
Load:
Detection:
AP-1 Glass Column,

10 x 100 mm
20 mM Tris-HCl pH 8.2
Buffer A with 1M Sodium Chloride
1.56 mL/min
0 to 25% Buffer B over 38 min
0.5 mg protein
280 nm
1. Adenosine
2. Carbonic anhydrase
3. Human transferrin
4. Ovalbumin
5. Soybean trypsin inhibitor
2
3
5
4
40 Min.
Protein-Pak PW Series Columns

Waters also offers a line of 10 m polymer-based ion-exchangers prepacked
in steel or glass columns. The Protein-Pak 5PW columns are available as
DEAE and SP ion-exchangers. These columns can be used on HPLC and FPLC
systems in both analytical and preparative configurations.
Note: Additional custom column dimensions are available.

Description
Approximate Protein Binding Capacity per Prepacked Column*

Prepacked Column
5 x 50 mm
5 x 100 mm
10 x 100 mm
Q**
60 mg
130 mg
500 mg
Protein-Pak HR Packing
DEAE**
SP***
40 mg
150 mg
300 mg
40 mg
80 mg
300 mg
CM***
Polymeric Weak Anion-Exchanger

Protein-Pak DEAE 5PW Glass Column
Protein-Pak DEAE 5PW Steel Column
Polymeric Strong Cation-Exchanger
Protein-Pak SP 5PW Glass Column
Dimensions
Part No.
7.5 x 75 mm
8 x 75 mm
21.5 x 150 mm
7.5 x 75 mm
8 x 75 mm
WAT088044
WAT011783
WAT010640
WAT088043
WAT011784
25 mg
45 mg
180 mg
Properties of Protein-Pak HR* Columns

Protein-Pak
Protein-Pak Q HR **
Type of material
Polymer
Protein binding capacity
60 mg/mL
Ion-exchange capacity
200 eq/mL
Nominal pK
11.7
Typical protein recovery
>95%
Typical recovery of biological activity >90%
pH stability
2-12
www.waters.com
Protein-Pak DEAE HR **
Protein-Pak CM HR ***
SP HR ****
Polymer
40 mg/mL
250 eq/mL
9.0
>95%
>90%
2-12
Polymer
25 mg/mL
175 eq/mL
5.7
>95%
>90%
2-12
Polymer
40 mg/mL
225 eq/mL
2.2
>95%
>90%
2-12
For best resolution do not exceed 20% of the protein

binding capacity.
**
Bovine serum albumin in 20mM Tris/Cl pH 8.2

was used to measure protein binding capacity of
Protein-Pak Q and DEAE HR.
***
Cytochrome c in 25mM MES pH 5.0 was used to

measure protein binding capacity of Protein-Pak SP
and CM HR.
****
Same conditions as CM. Protein binding capacity

of Protein-Pak SP 40 HR is 20 mg/mL.
227

Advanced Purification (AP) Glass Columns
Waters Advanced Purification (AP) series of glass columns are constructed

of biocompatible glass and polymeric materials and can be easily used with
silica, polymer, or soft gel packings. To optimize flow and ensure uniform
sample distribution onto the packed bed, eac h column incorporates a
distributor. A replaceable filter protects the packing from large particulate
contaminants. Empty AP glass columns are available in a variety of sizes
and utilize the same design to ensure predictable methods transfer
among them. AP glass columns are compatible with both analytical and
preparative HPLC and FPLC systems.
Dimensions
Bed Volume
(mL)
Flow Rate
(mL/min)
Pressure Rating
(psi/MPa)
Part No.
5 x 50 mm
5 x 100 mm
10 x 100 mm
10 x 200 mm
10 x 300 mm
10 x 600 mm
20 x 100 mm
20 x 200 mm
20 x 300 mm
20 x 600 mm
50 x 100 mm
50 x 200 mm
50 x 300 mm
50 x 600 mm
0.8-1.2
1.8-2.2
5-8
13-16
21-24
45-48
22-31
53-62
85-94
179-188
137-196
333-392
530-589
1118-1177
0-4
0-4
0-4
0-4
0-4
0-4
4-16
4-16
4-16
4-16
16-100
16-100
16-100
16-100
1500 psi/10 MPa

1500 psi/10 MPa
1500 psi/10 MPa
1500 psi/10 MPa
1500 psi/10 MPa
1500 psi/10 MPa
1000 psi/6.8 MPa
1000 psi/6.8 MPa
1000 psi/6.8 MPa
1000 psi/6.8 MPa
500 psi/3.4 MPa
500 psi/3.4 MPa
500 psi/3.4 MPa
500 psi/3.4 MPa
WAT064-01
WAT064-02
WAT021901
WAT021902
WAT021903
WAT021906
WAT027501
WAT027502
WAT027503
WAT027506
WAT023331
WAT023332
WAT023333
WAT023336
Additional connectors and fitting are available.
Advanced Purification (AP) Glass Column Accessories and Spare Parts

Waters Advanced Purification (AP) glass columns feature non-metallic
construction and adjustable bed height with easy-to-use coarse and
fine adjustments. T he AP glass columns are available in a variety
of dimensions.
AP Minicolumn Accessories and Spare Parts
Dimensions
Part No.
Glass Tube
5 x 50 mm
5 x 100 mm
5 x 50 mm
5 x 100 mm
WAT038802
WAT038803
WAT038804
WAT038805
WAT038806
Filters, 10/pk
O-rings, 13/pk
(includes 10 inlet/outlet and 3 funnel)
Inlet Connector Assembly
Description
Dimensions
Part No.
Glass Tube
20 x 100 mm
20 x 200 mm
20 x 300 mm
20 x 100 mm
20 x 200 mm
20 x 300 mm
WAT019891
WAT019892
WAT019893
WAT027542
WAT027543
WAT027544
WAT027528
WAT027530
Plastic Shield
Description
Column Jacket
AP-2 Column Accessories and Spare Parts
WAT038807
O-rings, 5/pk
Filters, 2/pk
Replacement Tubing (Tefzel)
1/8 inch o.d. x 0.040 inch i.d. x 10 feet
(3.2 mm o.d. x 1.02 mm i.d. x 3 m)
Distributors/inserts, 5/pk
WAT023344
WAT027525
700004715
WAT038800
Description
Dimensions
Part No.
Description
Dimensions
Part No.
Glass tube
10 x 100 mm
10 x 200 mm
10 x 300 mm
10 x 600 mm
10 x 100 mm
10 x 200 mm
10 x 300 mm
10 x 600 mm
WAT021992
WAT022033
WAT022034
WAT022035
WAT021927
WAT021945
WAT021946
WAT021947
WAT021907
WAT021910
Glass Tube
50 x 100 mm
50 x 200 mm
50 x 300 mm
50 x 100 mm
50 x 200 mm
50 x 300 mm
50 x 600 mm
WAT019876
WAT019877
WAT019878
WAT023370
WAT023371
WAT023372
WAT023373
WAT023345
WAT023343
WAT023344
Plastic shield
O-rings, 5/pk
Filters, 10/pk
Replacement tubing (Tefzel)
1/16 inch o.d. x 0.009 inch i.d. x 10 feet
(1.6 mm o.d. x 0.23 mm i.d. x 3 m)
WAT021950
Plastic Shield
O-rings, 5/pk
Filter, 2/pk
Replacement Tubing (Tefzel)
1/8 inch o.d. x 0.040 inch i.d. x 10 feet)
(3.2 mm o.d. x 1.02 mm i.d. x 3 m)
WAT023349
WAT021904
Consult your appropriate Care and Use Manual for additional spare parts information at www.waters.com/chemcu
228
www.waters.com
Fittings and Connectors for Advanced Purification (AP) Glass Columns

Connection of an AP Minicolumn and an AP-1 Column to 1/8 o.d. Tubing
1
Description
1 Collet and nut assembly (3/8-24)
2 Ferrule 1/8 tube
3 Union 3/8-24 x Z fitting
Qty.
Part No.
10/pk
10/pk
5/pk
WAT005138
WAT005136
WAT005137
Connection of an AP-2 and an AP-5 Column to 1/8 o.d. Tubing

1

2 Ferrule 1/8 tube
3 Union 3/8-24 x 3/8-24
1 Compression screw and ferrule Z fitting, plastic

2 Union Z fitting to Z fitting, plastic
1/pk
1/pk
WAT082708
WAT082745
Connection of Pharmacia Fitting to 1/16 o.d. Tubing

1
Qty.
Part No.
10/pk
10/pk
1/pk
WAT005138
WAT005136
WAT082734
Connection of a Protein-Pak Steel Column to 1/16

and 1/8 o.d. Tubing
3
Description
Qty.
Part No.
1 Compression screw and ferrule Z fitting, plastic

2 Union, plastic
1/pk
1/pk
WAT082708
WAT021951
Connection of 1/8 or 1/16 Flanged Type Fitting

to 1/8 o.d. Tubing
Description
Collet and nut assembly (3/8-24)
Ferrule 1/8 tube
Union 3/8-24 x Z fitting
Compression screw and ferrule Z fitting, plastic
Compression screw Z fitting, steel
Ferrule 1/16 steel
www.waters.com
Part No.
Description
1
2
3
4
5
6
Qty.
Description
Qty.
Part No.
10/pk
10/pk
5/pk
1/pk
10/pk
10/pk
WAT005138
WAT005136
WAT005137
WAT082708
WAT005070
WAT005063
Description
2 Ferrule 1/8 tube
Qty.
Part No.
10/pk
10/pk
WAT005138
WAT005136
229

Accell Plus Ion-Exchange Packings
Solid-phase extraction for protein sample preparation
Waters Accell Plus ion-exchange packings are 40 m, 300 polymercoated, silica-based materials for both lab- and process-scale chromatography. Accell Plus, available as a QMA strong anion exchanger or
CM, a weak cation exchanger, is easy to pack and is excellent for the
purification of proteins, enzymes, and immunoglobulins. The rigid
silica-based packing material will withstand very high flow rates during
cleaning and re-equilibration cycles. Normal flow rates are used during
sample loading and elution to obtain the best possible resolution.
Accell Plus bulk material may be packed into our Advanced Purification
(AP) glass columns. Prepacked 0.7 mL Sep-Pak cartridges can be used for
rapid method screening or solid phase extraction applications.
To estimate packed bed volume for a known amount of Accell Plus:
Ion-Exchange Sample Preparation

with Sep-Pak Cartridges
To perform ion-exchange sample preparation with Sep-Pak cartridges, use
a gradient of pH or ionic strength with Accell Plus CM, Accell Plus QMA or
NH2 as a sorbent.
Condition the cartridge with six to ten hold-up volumes

of deionized water or weak buffer
Load the sample dissolved in a solution of deionized

water or buffer
Elute unwanted weakly bound components

with a weak buffer
Elute the first component of interest with a stronger

buffer (change the pH or ionic strength)
Elute other components of interest with progressively

stronger buffers
W hen you recover all of your components, discard

the used cartridge in an appropriate manner
Accell Plus used (g) x 2 = packed bed volume (mL).
Accell Plus PrepPak Cartridges*

(47 x 300 mm)
Economical, convenient preparative separations in the 500 mg to
10 g range. For a complete listing of Waters products for preparative
chromatography, go to www.waters.com.
Particle
Description
Particle
Size
Accell Plus CM *
40 m
PrepPak 1000 Module
Pore
Size
Part No.
300
General Elution Protocol for Ion-Exchange Chromatography on

Sep-Pak Cartridges (NH2, Accell Plus QMA, Accell Plus CM)
Basic Steps for SPE
WAT036545
WAT089592
Condition/
Equilibrate
Load Sample
(Black)
3
Step
Elute 1
Step
Elute 2
Step
Elute 3
* Requires PrepPak 1000 Module
Protein Binding Capacity* of Accell Plus

Accell Plus QMA**
200 mg BSA/g packing
Accell Plus CM***

175 mg Cytochrome c/g packing
For best resolution do not exceed 20% of the protein binding capacity.
**
Bovine serum albumin in 20 mM Tris/Cl pH 7.0 was used to measure protein binding capacity
of Accell Plus QMA.
***
Cytochrome c in 20 mM sodium phosphate pH 6.3 was used to measure protein binding

capacity of Accell Plus CM.
1. Condition and equilibrate the cartridge

(not required for normal-phase)
2. Load sample
3. Elute componentsincrease strength
of mobile phase in steps
Accell Plus Sep-Pak Cartridges
Accell Plus Ion-Exchange Bulk Packings

For all preparative isolations based on ionic interactions, particularly
proteins, enzymes, and immunoglobulins.
Particle
Description
Particle
Size
Pore
Size
Accell Plus QMA

Anion Exchanger
Accell Plus CM
Cation Exchanger
40 m
300
40 m
300
230
Qty.
Part No.
100 g
500 g
100 g
500 g
WAT010742
WAT010741
WAT010740
WAT010739
Sep-Pak Plus cartridges packed with Accell Plus ion exchangers provide a
rapid, economical means to clean up heavily contaminated samples that
would damage a high resolution column. They can also be used to rapidly
screen chromatographic conditions. These are also available in a variety
of configurations.
Description
Accell Plus CM
Accell Plus QMA
Ion-Exchange Type
Part No.
Weak Cation Exchanger

Strong Anion Exchanger
WAT020550
WAT020545
www.waters.com

BioSuite Size-Exclusion HPLC Columns (SEC)
determine separation efficiency. BioSuite 4 m particle size, UHR (UltraHigh Resolution) columns provide maximum separation efficiency, followed
by BioSuite HR (High Resolution) columns and BioSuite Standard SEC
columns. To maximize column life of analytical (i.e., 4.6 mm or 7.8 mm
i.d.) or preparative (i.e., 21.5 mm i.d.) SEC columns, use of BioSuite Guard
columns is recommended.
BioSuite Ultra High Resolution (UHR), High Resolution (HR), and Standard
size-Exclusion Column packings use a rigid yet wettable silica-based
media that is stable from pH 2.5-7.5. As indicated in the calibration curve
tables, the exclusion limits of BioSuite SEC packings are determined by
the particle and pore size of the silica-based material. Particle size of the
SEC packing media as well as column length are important parameters that
LC/MS Analysis of BSA Aggregation Using BioSuite 250, 5 m HR SEC Column

100
100
TIC
66420
%
66443
%
66548
62801
68030
mass
60000
80000
100000
120000
140000
132835
100
0
10
12
14 Min.
132991
LC/MS:
Waters Alliance HPLC with 2996 PDA and ZQ (top)

or Q-Tof Mass Detectors (bottom)
BSA (heat aggregated)
BioSuite 250, 5 m, HR SEC (7.8 x 300 mm)
10 L
50 mM Ammonium formate (pH 6.5)
1 mL/min
25 C
ESI (positive)
3.3
30
400 3000 m/z
Sample:
Column:
Injection:
Mobile Phase:
Flow Rate:
Temp:
MS Source:
MS Capillary (kV):
MS Cone (V):
Mass Range:
66417*
%
131011
66506
66555
65504
64540
60000
133095
133175
135141
67505
67648
70000
80000
90000
100000
110000
120000
130000
mass
140000
LC/MS Analysis of Protein Standards Using BioSuite 250, 5 m High Resolution (HR) SEC Column (LC/MS conditions as above)
4
100
6
3
1.
2.
3.
4.
5.
6.
T hyrogobulin
BSA Dimer
BSA
-Lactoglobulin
Myoglobin
Cytochrome C
669 KDa
136 KDa
66 KDa
36 KDa
16 KDa
12 KDa
2
1
10
12
14
16
18
20 Min.
BioSuite SEC Reference: SEC- MS Analysis of Aggregates in Protein Mixtures. Application Book Supplement
of LC/GC Europe. Sept. 2003. (Waters Literature Code Number: 720000743EN)
www.waters.com
231

Protein Calibration Curves for BioSuite Ultra-High Resolution (UHR) SEC Columns
1,000,000
Sample:
BioSuite 250,
4 m UHR SEC
Columns:
Eluent:
Flow rate:
Temperature:
Detection:
Molecular
MWWeight
100,000
10,000
BioSuite 125,
4 m UHR SEC
T hyroglobulin (MW 670,000 Da), Gamma globulin (MW 155,000 Da),

Bovine Serum Albumin (66,330Da), Beta Lactoglobulin (MW18,400 Da),
Lysozyme (14,300 Da), Cytochrome c (12,400 Da),Triglycine (189 Da)
BioSuite 250, 4 m UHR SEC (4.6 x 300 mm)
BioSuite 125, 4 m UHR SEC (4.6 x 300 mm)
0.15 M Sodium Phosphate, pH 6.8
0.35 mL/min
25 C
280 nm (220 nm for triglycine)
Column
Globular Protein
Mol. Wt. Range
Branched
Dextrans
Linear
PEG/PEO
BioSuite 125
BioSuite 250
5,000-150,000
10,000-500,000
1,000-30,000
2,000-70,000
500-15,000
1,000-35,000
1,000
Note: Operating flow rates for BioSuite Ultra-High Resolution (UHR) SEC columns (4.6 mm i.d.) are from
0.1 - 0.4 mL/min. Use of an HPLC system (e.g. Waters Alliance HPLC System) capable of operating at these
flows is essential for optimal UHR SEC column performance.
100
6
10
12 MIn.
Protein Calibration Curves for BioSuite High Resolution (HR) SEC Columns
Sample:
BioSuite 250,
5 m HR SEC
10 6
BioSuite 450,
8 m HR SEC
Eluent:
Flow rate:
Temperature:
Detection:
10 5
Molecular Weight
Columns:
10 4
Column
BioSuite 125,
5 m HR SEC
BioSuite 125
BioSuite 250
BioSuite 450
10 3
T hyroglobulin (MW 670,000 Da), IgG (MW 156,000 Da),

BSA (66,330Da), Ovalbumin (MW 43,000 Da), Peroxidase
(40,200 Da),Beta Lactoglobulin (MW18,400 Da), Myoglobin
(MW 16,900 Da), Ribonuclease A (MW 13,700 Da),
Cytochrome C (12,400 Da), Glycine tetramer (246 Da)
BioSuite 450, 8 m HR SEC (7.8 x 300 mm)
0.1 M Sodium Phosphate, pH 7.0 containing 0.3M Sodium Chloride
1.0 mL/min
25 C
220 nm
Globular Protein
Mol. Wt. Range
Branched
Dextrans
Linear
PEG/PEO
5,000-150,000
10,000-500,000
20,000-7,000,000
1,000-30,000
2,000-70,000
4,000-500,000
500-15,000
1,000-35,000
2,000-250,000
10 2
232
10
12 MIn.
www.waters.com

Protein Calibration Curves for BioSuite Standard SEC Columns
BioSuite 250,
10 m SEC
Sample:
10 6
Columns:
Eluent:
Flow rate:
Temperature:
Detection:
BioSuite 450,
Molecular Weight
10 5
13 m SEC
10 4
T hyroglobulin (MW 670,000 Da), IgG (MW 156,000 Da),

BSA (66,330Da), Ovalbumin (MW 43,000 Da), Peroxidase
(40,200 Da),Beta Lactoglobulin (MW18,400 Da), Myoglobin
(MW 16,900 Da), Ribonuclease A (MW 13,700 Da),
Cytochrome C (12,400 Da), Glycine tetramer (246 Da)
BioSuite 450, 13 m SEC (7.5 x 300 mm)
0.1M Sodium Phosphate, pH 7.0 containing 0.3M Sodium Chloride
1.0 mL/min
25 C
220 nm
Column
BioSuite 125
BioSuite 250
BioSuite 450
Globular Protein
Mol. Wt. Range
Branched
Dextrans
Linear
PEG/PEO
5,000-100,000
10,000-500,000
20,000-7,000,000
1,000-30,000
2,000-70,000
4,000-500,000
500-15,000
1,000-35,000
2,000-250,000
BioSuite 125,
10 m SEC
10 3
7.5
10 Min.
BioSuite 4.6 mm, 7.8 mm, and 7.5 mm i.d. Analytical

or 21.5 mm i.d. Preparative Columns are Available.
Globular
Column
Volume
Suggested Protein
Mass Load for Maximum
Protein Mol. Wt.
Range
Suggested Volume
Load for Maximum
Multicomponent
Resolution*
Multicomponent
Resolution*
Description
Matrix
Inner
Diameter
Length
BioSuite 125, 4 m UHR SEC

BioSuite 250, 4 m UHR SEC
BioSuite UHR Guard SEC
Silica
Silica
Silica
4.6 mm
4.6 mm
4.6 mm
300 mm
300 mm
35 mm
4.98 mL
4.98 mL
5,000-150,000
10,000 - 500,000
Less than 8 mg/mL

Less than 8 mg/mL
Less than 40 L
Less than 80 L
186002161
186002162
186002163
BioSuite 125, 5 m HR SEC

BioSuite HR Guard SEC
Silica
Silica
Silica
Silica
7.8 mm
7.8 mm
7.8 mm
6 mm
300 mm
300 mm
300 mm
40 mm
14.33 mL
14.33 mL
14.33 mL
5,000-150,000
10,000 - 500,000
20,000-7,000,000
Less than 8 mg/mL

Less than 8 mg/mL
Less than 8 mg/mL
Less than 200 L

Less than 200 L
Less than 200 L
186002164
186002165
186002166
186002167
BioSuite 125, 10 m SEC

BioSuite Guard SEC
BioSuite Guard SEC
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
7.5 mm
21.5 mm
7.5 mm
21.5 mm
7.5 mm
21.5 mm
7.5 mm
21.5 mm
300 mm
300 mm
300 mm
300 mm
300 mm
300 mm
75 mm
75 mm
13.25 mL
108.9 mL
13.25 mL
108.9 mL
13.25 mL
108.9 mL
5,000-150,000
5,000-100,000
10,000 - 500,000
10,000 - 500,000
20,000-7,000,000
20,000-7,000,000
Less than 8 mg/mL

Less than 8 mg/mL
Less than 8 mg/mL
Less than 8 mg/mL
Less than 8 mg/mL
Less than 8 mg/mL
Less than 200 L

Less than 1.6 mLs
Less than 200 L
Less than 1.6 mLs
Less than 200 L
Less than 1.6 mLs
186002168
186002169
186002170
186002171
186002172
186002173
186002174
186002175
Part No.
* Using a BSA protein standard in a 50 mM phosphate buffer containing salt (either 0.1 M NaCl or 0.1 M Na2SO4 ) eluent.
Useful protein mass loads will vary depending upon separation eluent, complexity of sample, and on the type of proteins contained in mixture.
In general, maximum component resolution is obtained by injecting the smallest possible volume of a dilute protein solution.
* Note: Operating flow rates for BioSuite Ultra-High Resolution (UHR) SEC columns (4.6 mm i.d.) are from 0.1-0.4 mL/min. Use of an HPLC system
(e.g. Waters Alliance HPLC System) capable of operating at these flows is essential for optimal UHR SEC column performance.
www.waters.com
233

Protein-Pak and Shodex Size-Exclusion HPLC Columns
Waters offers two families of packings for size-exclusion chromatography. Protein-Pak packings are based on a 10 m diol-bonded silica and
are available in a selection of pore sizes and column configurations.
In addition, Waters offers a series of Shodex 7 m high-resolution, gel
filtration packings.
The Protein-Pak size-exclusion columns can be expected to resolve proteins
that differ in molecular weight by a factor of two and to distinguish
proteins differing by as little as 15% in molecular weight. The degree of
resolution is more dependent on the sample mass and volume than the
interaction between the sample and the stationary phase. Ideally, there
should be no interaction between the stationary phase and the sample
molecules. Secondary interactions are most often ionic and can, therefore,
be reduced by increasing the ionic strength of the mobile phase. Typical,
salt concentrations range to 0.2-0.5M NaCl. It may also be useful in
some cases to consider adding 10-20% methanol to eliminate hydrophobic and other hydrogen-bonding interactions.
Protein-Pak Columns and Packings
Steel Column
Dimensions
Protein-Pak 60
7.8 x 300 mm
Protein-Pak 125
7.8 x 300 mm
Protein-Pak 300SW
7.5 x 300 mm
Protein-Pak 125 Sentry Guard Column
3.9 x 20 mm, 2/pkg (requires holder)
Glass Column
Protein-Pak 200SW
8 x 300 mm
Protein-Pak 300SW
8 x 300 mm
Inquire for additional offerings including prep.
Standard Protein Mix on KW-803 Column

Column:
Eluent:
Flow Rate:
Detection:
Protein KW-803
25 mM Sodium Phosphate pH 6.8
0.72 mL/min
280 nm
1. Blue Dextran
2. Ferritin
3. Aldolase
4. Bovine Serum Albumin
5. Ovalbumin
6. Chymotrypsinogen
7. Cytochrome c
8. Cytidine
7
6
3
4 5
25 Min.
This gel filtration separation of protein standards demonstrates the ability to separate proteins in a wide range of
molecular weights in minutes for high sensitivity analysis or protein isolation up to the milligram scale.
Shodex Size-Exclusion Columns

MW Range
Part No.
1,000-20,000
2,000-80,000
10,000-300,000
WAT085250
WAT084601
WAT080013
186000926
WAT046910
500-60,000
10,000-300,000
Column
Dimensions
Protein KW-802.5
8 x 300 mm
Protein KW-803
8 x 300 mm
Protein KW-804
8 x 300 mm
Protein-Pak 125 Sentry Guard Column
3.9 x 20 mm, 2/pkg (requires holder)
Particle Size
MW Range
Part No.
7 m
7 m
7 m
100-50,000
100-150,000
500-600,000
WAT035943
WAT035946
WAT036613
186000926
WAT046910
WAT011786
WAT011787
Ultrahydrogel HPLC Columns

Packed wit h hydroxylated polymet hacrylate-based gel, Waters
Ultra hydrogel SEC columns are ideal for the analysis of aqueoussoluble samples, suc h as oligomers; oligosacc harides; polysacc harides;
and cationic, anionic, and amphoteric polymers. Measuring 7.8 x 300 mm,
these high-resolution columns offer many advantages over conventional
aqueous SEC columns, such as:
Ultra hydrogel Columns (7.8 x 300 mm)

Column
Pore Size
103
A wide pH range (2-12)
Compatibility with high concentrations of organic solvents

(up to 20% organic, 50% organic if the mobile phase is
introduced by gradient)
Greater flexibility for the mobile phase
* DP = Degree of Polymerization, choice of column when working with glucose oligomers.
Minimal non-size-exclusion effects
5x
8 x 104
4 x 105
1 x 106
7 x 106
7 x 106
5 x 103
N/A
N/A
Part No.
Ultrahydrogel 120
Ultrahydrogel 250
Ultrahydrogel 500
Ultrahydrogel 1000
Ultrahydrogel 2000
Ultrahydrogel Linear
Ultrahydrogel DP*
Ultrahydrogel Guard Column DP*
234
120
250
500
1000
2000
Blend
120
N/A
N/A
Exclusion Limit
WAT011520
WAT011525
WAT011530
WAT011535
WAT011540
WAT011545
WAT011550
WAT011565
WAT011570
www.waters.com
Symmetry300 C4 HPLC Columns

Compared to our BEH300 C4 offerings, Symmetry300 C4 particles are
100% silica-based and are synthesized using ultrapure organic reagents
resulting in high purity material with very low silanol activity for outstanding peptide and protein separations and recoveries.
Protein: Symmetry300 C4 Versus Competitors
Mobile Phase A:
Mobile Phase B:
Flow Rate:
Gradient:
0.1% TFA in water

0.75 mL/min
Time
Profile
(min)
%A
%B
0.00
80
20
15.00 35
65
Injection Volume:
Temperature:
Detection:
Instrument:
Analytes:
20 L
40 C
UV @ 220 nm
Alliance 2790, 996 PDA
1. RNAse
2. BSA
3. B-Lactoglobulin
4. Ovalbumin
Competitive 300
C18 column
P = 90.09
4
2
10
12
300 pore for peptide and protein applications
Fully end-capped to minimize undersired secondary interactions
Alternative separation selectivity compared to

Waters BEH300 C4 hybrid material
QC tested with peptide samples to help ensure excellent

batch-to-batch consistency
Symmetry300 C4 Columns
Symmetry300
C4 column
P = 138.56
14 Min.
Inner
Description
Diameter
Particle
Length
Size
Part No.
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
Symmetry300 C4
1.0 mm
2.1 mm
2.1 mm
2.1 mm
4.6 mm
4.6 mm
4.6 mm
4.6 mm
2.1 mm
3.9 mm
4.6 mm
4.6 mm
4.6 mm
150 mm
50 mm
100 mm
150 mm
50 mm
75 mm
100 mm
150 mm
150 mm
150 mm
50 mm
150 mm
250 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
5 m
5 m
186000276
186000277
186000278
186000279
186000280
186000281
186000282
186000283
186000285
186000286
186000287
186000288
186000289
Protein-Pak Affinity Columns

T he Protein-Pak Affinity Epoxy-Activated packing consists of 40 m, 500
pore size particles having a hydrophilic bonding layer with a glycidoxypropyl functionality resulting in a seven atom spacer arm. T he epoxy-activated
surface can immobilize a wide range of ligands via a covalent linkage with
amino, hydroxyl or sulfhydryl groups using simple coupling procedures.
For method screening or small scale separation, choose the convenience of
prepacked microcolumns. Larger-scale separations are easily achieved by
packing bulk material in our Advanced Purification (AP) glass column.
To estimate packed bed volume for a known amount of Protein-Pak
Affinity Epoxy-Activated packing:
Protein-Pak Affinity Epoxy-Activated used

(g) x 2 = packed bed volume (mL).
Particle
Packing
Particle
Size
Protein-Pak Affinity
40 m
Epoxy-Activated Packing
Protein-Pak Affinity
Epoxy-Activated Microcolumn 40 m
(500 mg of material in a 3 cc syringe barrel)
Pore
Size
Purification of Carbonic Anhydrase

Ligand:
Column:
Buffer A:
Buffer B:
Gradient:
Detection:
Protein Load:
Sulfanilamide, 180 moles/g

AP-1 Glass Column, 10 x 100 mm
100 mM Tris sulfate with 200 mM sodium sulfate pH 8.7
200 mM potassium thiocyanate in 50 mM Tris sulfate pH 6.5
Initial
A
B
Flow
1 min 100% 0%
1 mL/min
2 min 100% 0%
2 mL/min
7 min
0%
100% 2 mL/min
17 min 100% 0%
2 mL/min
280 nm
24 mg of bovine hemolysate yielded 0.6 mg of carbonic anhydrase
with a 100% recovery of esterase activity.
1. Carbonic Anhydrase
Qty.
Part No.
500
25 g
100 g
WAT030653
WAT030654
500
10/box
WAT035955
30 Min.
Inquire for additional offerings.
www.waters.com
235

BioSuite pC18 and pPhenyl Reversed-Phase Chromatography (RPC) HPLC Columns
Reversed-phase chromatography (RPC) has become a widely accepted
tool for the separation of proteins, peptides, synthetic oligonucleotides, and other biomolecules. For many applications, Symmetry
and Symmetry300, Atlantis, T3, or XBridge BEH130 and BEH300
chemistries can be successfully used for the isolation and analyses of
these biocompounds. However for some applications, the large pore size
and high chemical stability of BioSuite pC18 and pPhenyl resin-based
packings may be preferred. BioSuite RPC column offerings include a
C18 (pC18) and a phenyl (pPhenyl) chemistry bonded to a pH stable,
methacrylic ester-based polymeric resin. The 500 pore size of the
pC18 base matrix accommodates proteins up to 2,500,000 Daltons
while the 1,000 pore size of the pPhenyl base matrix accommodates
proteins up to 5,000,000 Daltons.
The BioSuite pC18, 2.5 m, NP column contains a non-porous chemistry
that yields superior chromatographic resolution in less time compared
to chromatography performed on the porous, pC 18, 500, 7 m RPC
selection. Waters porous, pC18, 500, 7 m RPC column is available for
applications requiring greater binding capacity. The pC18 and pPhenyl
RPC chemistries are available in 21.5 x 150 mm columns for labscale isolations while a 2.0 x 75 mm column is well suited for narrowbore HPLC and LC/MS applications.
Reversed-phase Chromatography at Elevated pH on BioSuite pC18

RP Column Possible on Polymer Based Material
Hydrophobic Proteins are Well Resolved by Reversed-phase

Chromatography on BioSuite pPhenyl RP Column
BioSuite pPhenyl, 1000, 10 m RPC
Sample:
Ribonuclease (Peak 1), bovine Insulin (Peak 2bovine), Cytochrome-C

(Peak 3), Lysozyme (Peak 4), Transferrin (Peak 5), Bovine serum
albumin (Peak 6), Myoglobin (Peak 7), Ovalbumin (Peak 8)
BioSuite pPhenyl, 1000, 10 m RPC (4.6 x 75 mm)
HPLC grade water with 0.05% TFA
Acetonitrile with 0.05% TFA
1
2
1.0 mL/min
3
25 C
220 nm
Column:
Eluent: A:
Eluent B:
Flow rate:
Gradient:
Temperature:
Detection:
Column:
Eluent: A:
Eluent B:
Flow rate:
Gradient:
Temperature:
Detection:
Met-enkephalin (Peak 1), bradykinin (Peak 2), leu-enkephalin (Peak 3),

Neurotensin (Peak 4), bombesin (Peak 5), Angiotensin l (Peak 6),
Somatostatin (Peak 7), Insulin (Peak 8)
BioSuite pC18, 500, 7 m RPC (4.6 x 150 mm)
0.2 M NH4OH, pH 10.8
0.2 M NH4OH, pH 10.8 (20%) / Acetonitrile (80%)
1.0 mL/min
3
25 C
1
6
220 nm
6
8
30 Min.
LC/MS Analysis of a Reduced Monoclonal IgG1

Antibody on a BioSuite pPhenyl RPC Column
Sample:
Column:
Eluent A:
Eluent B:
Flow Rate:
Gradient:
Temperature:
Detection:
Reduced monoclonal IgG1 antibody

BioSuite pPhenyl, 1000 A, 10 m RPC (2.0 x 75 mm)
Water with 0.1% Formic Acid
Acetonitrile with 0.1% Formic Acid
0.2 ml/min
10% B for 6 min (waste), then 20-35% B in 30 min
80C
Waters LCT Premier ESI-TOFMS
LC
HC
TIC of
Reduced IgG1
antibody
7
15
Peak No.
1
2
3
4
5
6
7
8
9
10
BioSuite pC18, 500, 7 m RPC

Sample:
Identity
HC Clip1 (with glycan variants)
LC (1-2 S-S Intact)
LC
LC+1 Da
HC Clip 2 (w/o glycan variants)
HC Clip 3 (w/o glycan variants)
Antibody Half mer
HC (with glycan variants)
HC Fragment (w/o glycan variants)
HC Fragment-18 Da
(w/o glycan variants)
HC: Heavy Chain, LC: Light Chain

TIC: Total Ion Chromatogram
BioSuite pPhenyl,
1000 A, 10 m RPC
2
9
7
10
15
30 Min.
Note: The BioSuite pPhenyl, 1000 RPC columns have a higher ligand
density compared to the BioSuite Phenyl, 1000 HIC columns and
are not recommended for hydrophobic-interaction separations.
236
10
12
14
16
Description
18
20
22
24
Matrix Inner Diameter
26
28
30 Min.
Length
Part No.
BioSuite pC18, 2.5 m NP RPC

Polymer
Polymer
Polymer
Polymer
4.6 mm
2.0 mm
4.6 mm
21.5 mm
35 mm
150 mm
150 mm
150 mm
186002152
186002153
186002154
186002155

Polymer
Polymer
Polymer
2.0 mm
4.6 mm
21.5 mm
75 mm
75 mm
150 mm
186002156
186002157
186002158
www.waters.com
BioSuite Hydrophobic-Interaction Chromatography (HIC) HPLC Columns

The separation of proteins and peptides based upon hydrophobic characteristics is a powerful chromatographic technique. However, some proteins
denature at elevated organic solvent concentrations making reversed-phase
chromatography (RPC) difficult. BioSuite Phenyl HIC columns provide a
viable separation alternative to RPC. HIC is characterized by the adsorption
of compounds to a weakly hydrophobic surface at high salt concentrations,
followed by elution with a decreasing salt gradient. HIC combines the
non-denaturing characteristics of salt precipitation with the precision of
HPLC to yield excellent separation of biologically active material.
BioSuite Phenyl, 1000, 10 m HIC column media consists of a phenyl
group bonded to a methacrylic ester-based polymeric resin. The large
1000 pore size accommodates proteins up to 5,000,000 Daltons. A
21.5 mm i.d. x 150 mm column is also available for lab-scale isolations.
Note: T he BioSuite P henyl, 1000 HIC columns have a lower ligand
density compared to the BioSuite pPhenyl, 1000 RPC columns and are
not recommended for reversed-phase separations.
Description
BioSuite Phenyl 10 m HIC
BioSuite Phenyl 13 m HIC
Matrix
Inner
Diameter
Length
Part No.
Polymer
Polymer
7.5 mm
21.5 mm
75 mm
150 mm
186002159
186002160
Hydrophobic-Interaction Chromatography on BioSuite Phenyl HIC

Column is an Excellent Alternative to Reversed-phase Methods.
BioSuite Phenyl,
10 m HIC
Sample:
Cytochrome c (Peak 1),

Myoglobin (Peak 2), Ribonuclease
(Peak 3), Lysozyme (Peak 4),
Alpha-chymotrypsin (Peak 5), and
Alpha-Chymotrypsinogen A (Peak 6)
Column:
BioSuite Phenyl, 10 m HIC

(7.5 x 75 mm)
0.1 M Sodium Phosphate,
pH 7.0 containing
2 M Ammonium Sulfate
0.1 M Sodium Phosphate, pH 7.0
1.0 mL/min
Linear gradient from
0 to 100% B in 60 min.
25 C
280 nm
Eluent: A:
Eluent B:
Flow Rate:
Gradient:
Temperature:
Detection:
30
60 Min.
Protein HIC and Protein-Pak Phenyl HIC HPLC Columns

Waters Protein HIC and Protein-Pak Phenyl hydrophobic-interaction columns
are packed with rigid, 10 m polymethacrylate packing materials with
500 pore size to ensure rapid protein diffusion. A low density bonding
with phenyl groups results in a hydrophobic surface that allows for protein
purification with a high recovery of both mass and biological activity.
Protein Purification by Hydrophobic-Interaction Chromatography

Sample:
Column:
Buffer A:
Buffer B :
Gradient:
Flow Rate:
Detection:
Wheat Germ Extract

Protein HIC PH-814, 8 x 75 mm
1.7 M Ammonium sulfate in Buffer B
0.1M Sodium phosphate, pH 7.0
0-100% Buffer B in 15 minutes linear
1.1 mL/min
280 nm
HIC Packing Material Capacities

Protein HIC PH-814
Protein-Pak Phenyl 5PW
40 mg/mL *
25 mg/mL *
Lectin
* Ovalbumin in 1.8 M ammonium sulfate, 0.1 M sodium phosphate pH.
Hydrophobic-Interaction Columns
Description
Dimensions
Part No.
Protein HIC PH-814 Steel Column
8 x 75 mm
WAT035520
Inquire for additional offerings.
30 Min
Unique selectivity of hydrophobic interaction packings provides resolution

and recovery of biologically active proteins with little denaturation.
www.waters.com
237
aMINO aCId aNaLYSIS
Amino Acid Analysis

Overview
Amino Acid Analysis (AAA) is required for many applications. Amino acids
are the constituents of proteins and are intermediates in many metabolic
pathways. Bound amino acids are measured following hydrolysis of the
protein to determine the concentration of protein, to identify a protein, and
to detect structural variants. Amino acid composition is a critical component
of the nutritional value of foods and feeds. Free amino acids are measured
without hydrolysis using the same analytical tools used to monitor cell
culture and fermentation processes. Similar assays are applied in the food
industry to recognize the origin of the natural products based on the free
amino acids released by extraction. AAA is also used to assess the status
of metabolic pathways in clinical researc h laboratories. Eac h of these
applications requires specific sample-handling and some modification
to the method.
Sample Preparation
Samples for AAA can be divided into two groups. W here the protein in the
sample is the ultimate object of the analysis, the sample must be hydrolyzed to release the amino acids. In contrast, where the amino acids are
measured as metabolic indicators, no hydrolysis is required. However, the
amino acids may need to be separated from other sample constituents that
could bind amino acids or otherwise interfere with the analysis.
Purified proteins and peptides are hydrolyzed with acid at high temperature,
commonly 6N HCl at 100-105 C for 24 hours. Higher temperatures for
shorter times are sometimes used for specific purposes but with reduced
recovery and increased side reactions. Vapor-phase hydrolysis is useful for
sensitive analysis of small amounts of pure proteins.
T here are artifacts of hydrolysis. T he amine, asparagine, and glutamine,
are converted to the corresponding acids. Tryptophan is completely lost in
hydrolysis, and significant amounts of the sulfur-containing amino acids
are also destroyed. Some amino acids, e.g., threonine, serine, and tyrosine
may be oxidized while the bonds between hydrophobic residues are hydrolyzed slowly, leading to underestimates in both cases. Tryptophan may be
preserved with alkaline hydrolysis or with the use of methanesulfonic acid.
T he sulfur amino acids may be quantitatively oxidized with performic acid
to the very stable methionine sulfone and cysteic acid. Oxidation degrades
other amino acids, including tyrosine, serine, and threonine, so reduction
and alkylation of cysteine residues and carefully excluding oxygen from
the hydrolysis to preserve methionine is more useful. To correct for gradual
destruction or release, data from three hydrolysis times, for example, 24,
48, and 72 hours, are extrapolated to constant values.
238
Amino Acid Analysis
Food and feed samples for assessing nutritional content are much more
complex. Hydrolysis requires large volumes of 6N HCl to ensure both
dispersal of the sample and a sufficient excess of acid. T he sulfur-containing amino acids are particularly important in these assays because they
are growth-limiting. Performic acid oxidation is preferred for accurate
determination of methionine and cysteine in these complex samples.
T he most complete profile is obtained by analyzing the sample with and
without oxidation.
In cell culture and fermentations, free amino acids are supplied in the media
as nutrients. A larger group of amino acids, including especially glutamine
and tryptophan must be included in the measurement for assessing the
health of the cell culture and supporting a feeding schedule.
Analysis of Amino Acids

After hydrolysis or other sample preparation, the amino acids are ready
for analysis. T his is a c hallenging analytical problem because the set of
compounds covers a wide range of c hemical properties but with pairs of
components that are very similar. T hey also lack common chemical features
that can be used for convenient detection. Several suitable solutions have
been developed to address these concerns.
Amino acids were first separated by cation-exc hange c hromatography.
Decades of development and continual improvements have produced
methods that give complete resolution in less than 60 minutes. Ionexc hange methods are not compatible with direct detection. An added
post-column reaction system uses a colorimetric reagent like ninhydrin or
a fluorescent reactant like o-pthalaldehyde.
T he differences between the amino acids are more suitable for reversedphase c hromatography. Because most of the compounds are polar,
reversed-phase separations incorporate an ion-pairing reagent like a
perfluorinated acid. T hese separations may be coupled to an electrospray mass spectrometer for detection.
A more general approach to reversed-phase AAA is pre-column derivatization. T he derivatized amino acids retain better on the reversed-phase
column and can be more easily separated. Most common derivatization
reagents react with the amines. Some reagents react only with primary
amines, but the generally useful ones also react with secondary amines so
that proline is detected. In addition to improving chromatography, derivatization can make the amino acids readily detectable. T he detection may be
UV absorbance or fluorescence. Several reagents have been used, and two
of the most thoroughly developed and best understood are described below.
Both approaches to AAA yield satisfactory results. Ion exchange with postcolumn detection is the oldest and has the larger knowledge base about
unusual amino acids. Reversed-phase methods with pre-column derivatization are faster, more sensitive, and do not require specialized equipment.
www.waters.com
aMINO aCId aNaLYSIS
UPLC Amino Acid Analysis Solution
T he UPLC Amino Acid Analysis Solution

Over the years, Waters has provided three distinct products utilizing pre-column derivatization and reversed-phase HPLC: AutoTag,
PicoTag, and AccQTag. Today, Waters continues to lead the way by
bringing together its most ground-breaking and popular tec hnologies: ACQUIT Y UPLC and AccQTag Ultra, the newest addition to the
AccQTag family of products.
T he UPLC Amino Acid Analysis Solution is holistically designed and
optimized specifically for amino acid analyses. Derivatized amino acids
are separated using the ACQUIT Y UPLC System, enhancing resolution
to ensure accurate and precise qualitative and quantitative results. Just
as important, our solution provides performance-qualified methodologies whic h are designed to be rugged and reliable, assuring reproducible results day-to-day, instrument-to-instrument, lab-to-lab, around
the worldwith the expert support that scientists have come to expect
from Waters.
www.waters.com
T he UPLC Amino Acid Analysis Solution leverages Waters experience

in separation science, derivatization c hemistries, and information
management. It is a total application solution optimized for accurate,
reliable, and reproducible analysis of amino acids. Based on Waters
AccQTag Ultra c hemistry, combined with our award winning ACQUIT Y
UPLC separation tec hnology, users can feel confident with assured
performance in the areas of protein c haracterization, cell culture
monitoring, and nutritional analysis of foods and feeds.
T he UPLC Amino Acid Analysis Solution consists of:
Waters ACQUITY UPLC System and tunable UV detector (Optional

fluorescence and PDA detectors are also fully supported)
AccQTag Ultra
derivatization chemistries including column,

reagents, and eluents (all quality control tested)
Empower 2 pre-configured projects, methods, and report templates
Installation and application training and support included
Application-specific Performance Qualification
Connections INSIGHT Intelligent Services
Amino Acid Analysis
239
Amino Acid AnAlysis

Amino Acid Analysis Methods for the Analysis of
Hydrolysates and of Cell Culture Media
Chemistry of the AccQTag Derivatization Reaction

O
AMQ
Hydrolysates
Lle
Leu
Phe
Tyr
Met
Val
NVa
Deriv Peak
Cys
Pro
Ala
Thr
Glu
Asp
Arg
Gly
Ser
His
NH3
Lys
H
N
N
OH
O
O
O
AQC Reagent
1 Amino Acid:
t1/2<<1s
NH 2
OH
NH
Derivatized Amino Acids
2 Amino Acid:
t1/2<<1s
H
N
AMQ
Pro
Lle
Leu
Phe
Trp
Lys
N
O
O
HO
Cell Culture
Tyr
Met
Val
NVa
Deriv Peak
Cys
ABAA
Hy Lys 1
Hy Lys 2
Ala
GABA
Thr
Glu
Asp
Ser
Gln
Arg
Gly
AE
Tau
Orn
H2O
t1/2~15s
NH3
Hy Pro
His
Asn
NH
O
OH
H
N
N-Hydroxy
Succinimide
+ CO2
H
N
H
N
NH2
N
6-Aminoquinoline (AMQ)
bis-aminoquinoline urea (derivatization peak)
AccQTag Derivatization Reaction

1.50
3.00
4.50
6.00
7.50 Min.
The UPLC Amino Acid Analysis solution can be used for the analysis of protein hydrolysates or for
the larger set of amino acids found in cell culture media. The only differences in the methods are a
different dilution of the supplied eluent A concentrate and operation at a different temperature.
No method development or buffer reformulation is required.
AccQTag Ultra Chemistry
AccQTag Ultra Chemistry

Part No.
UPLC AAA Application Add-on Kit*

AccQTag Ultra Refill Package**
Refill Package Includes:

AccQTag Ultra Derivatization Kit, 250 Analyses

AccQTag Ultra Column. 2.1 x 100 mm

AccQTag Ultra Eluent A, Concentrate (950 mL)

AccQTag Ultra Eluent B (950 mL)

Sample Tubes, 4 x 72/pk

Amino Acid Standard, Hydrolysate, 10 x 1 mL ampules

Total Recovery Vials, 3 x 100 vial/pk
176001279
176001235
186003836
186003837
186003838
186003839
WAT007571
WAT088122
186000384C
* This kit is intended to enable existing ACQUITY UPLC systems for AAA applications.
The Add on Kit contains the AccQTag Ultra Chemistries and Column, Documentation
and additional hardware accessories needed for AAA applications.
** The Refill Kit is intended to recharge the AccQTag Ultra chemistries that are a part of
the Application Add on Kit. This kit should not be purchased as part of an initial system.
240
Amino Acid Analysis
6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
US Patent 5,296,599 and European
Patent EP 0 533 200 B1
Reacts rapidly with both primary and secondary amines
T he AccQTag Ultra chemistry products are a comprehensive, fully-tested

set of reagents, columns, and eluents optimized for use with the UPLC
Amino Acid Analysis Solution. T his chemistry is based on Waters widelyused and well-understood AccQTag derivatization method. Primary and
secondary amino acids are derivatized before separation with a single
reagent, AccQFluor, in a high-throughput batc h process resulting in
exceptionally stable derivatives. High resolution separations are achieved
using the pre-qualified AccQTag Ultra UPLC columns and mobile phases.
Derivatized amino acids are quantified to sub-pmol levels with single
wavelength UV detection.
Description
Utilizes AccQTag Ultra Reagent Powder
Excess reagent reacts more slowly with water to form a product
that can be separated chromatographically from the derivatized

amino acids
Requires no vacuum drying, sample prep, or extraction
Literature
References
HPLC and UPLC Amino
Acid Analysis Brochure,
Monitoring Cell Culture Media with the Waters

Amino Acid Analysis Solution,
UPLC Amino Acid

Analysis Solution Brochure,
UPLC Amino Acid Analysis Solution,

Amino Acid Analysis for Monitoring Cell

Culture Media and Protein Structure Poster,
Amino Acid Analysis for Monitoring
Protein Structure and for Measuring Protein
Concentration Poster,
ACQUITY UPLC for the Rapid

Analysis of Amino Acids in Wine,
Determination of Amino Acids in Beers Using
the UPLC Amino Acid Analysis Solution,
Application Solutions for Biopharmaceuticals,
Amino Acid Analysis of Pure Protein

Hydrolysates with Application of Waters UPLC
Amino Acid Analysis Application,
www.waters.com
Amino Acid AnAlysis
AccQTag Amino Acid Analysis Using HPLC

AccQTag Analysis of Hydrolysate Amino Acid
Sample:
Column:
Temperature:
Eluent A:
Eluent B:
Eluent C:
Flow Rate:
Gradient:
Detection:
AQC-Derivatized Amino Acid Standards

AccQTag Column, 3.9 x 150 mm
37 C
AccQTag Eluent A
Acetonitrile
HPLC grade water
1 mL/min
AccQTag Method
Fluorescence: lex = 250 nm, lem = 395 nm
19
17
18
14
15
13
T he HPLC AccQTag method utilizes pre-column derivatized reagents

that yield easily detected fluorescence adducts. The AccQFluor reagent,
6-aminoquinolyl-N-hydroxysuccinimidyl carbamate derivatizes primary and
secondary amines in a simple, single-step reaction to yield highly stable,
fluorescent adducts. We offer the AccQTag method as a system package
consisting of prepackaged reagents and extensive documentation.
T he AccQTag Chemistry Package contains the items you need for up to
250 analyses of protein and peptide hydrolysate amino acids.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
AMQ
Asp
Ser
Glu
Gly
His
NH3
Arg
Thr
Ala
Pro
Cys
Tyr
Val
Met
Lys
Ile
Leu
Phe
16
A. 50 pmol Standard
1
6
4 5
10
11
12
30 Min.
17
19
18
14
1
15
13
B. 1 pmol Standard
3
2
56
16
10
11
12
AccQFluor Reagent Kit (Five Bottles Each)

30 Min.
AccQFluor Borate Buffer
AccQFluor Reagent Powder
Application of the AccQTag Method to the analysis of hydrolysate amino acids is illustrated.
The high purity reagents provided in the AccQTag Chemistry Package enable high sensitivity
analysis by minimizing background amino acid content.
AccQFluor Reagent Diluent
* AQC-6-aminoquinolyl-N-hydroxysuccinimidyl, NHS-N-hydroxysuccinimidyl, AMQ-6-aminoquinoline
AccQTag Amino Acid Analysis Column

Separates the amino acid derivatives produced by the AccQFluor derivatization reaction. T he AccQTag column is a high-efficiency Column specifically certified for use with the AccQTag method. Care and use of the
column is described in the Waters AccQTag Amino Acid Analysis Column
Care and Use.
AccQTag Eluent A Concentrate
A premixed concentrated aqueous buffer.
Amino Acid Hydrolysate Standard
Ten 1 mL ampules of the Amino Acid Hydrolysate Standard. Each ampules
contains a 2.5 mM mixture of the 17 hydrolysate amino acids with the
exception of cystine (1.25 mM).
6 x 50 mm Sample Tubes
Used for preparing samples and standards.
AccQTag Chemistry Package Instruction Manual
Describes the AccQTag Amino Acid Analysis Method.
Injection Volume Guidelines

Sample
Estimated Sample Quantity*
Proteins
0.1 to 1.0 g 4 to 40 picomole

0.1 to 5.0 g 4 to 200 picomole
0.02 to 0.20 g 20 to 200 picomole
0.02 to 1.0 g 200 to 1000 picomole
50 picomole
Peptides
Standard
Injection Volume
10 to 20 L
5 to 10 L
10 to 20 L
5 to 10 L
5 L
*Based on protein average molecular weight=25,000, and peptide average molecular weight=1,000.
Column and Accessories
Dimensions/Qty.
AccQTag Chemistry Package for

up to 250 analyses. Includes:
AccQFluor Reagent 1
5 x 6 mL vials
AccQFluor Reagent 2A
5 x 3 mg vials
AccQFluor Reagent 2B
5 x 3 mL vials
AccQTag Column
3.9 x 150 mm
AccQTag Eluent A, Concentrate
2 x 1 liter
Sample tubes
4 x 72/pk
Amino Acid Standard, Hydrolysate
10 x 1 mL ampules
AccQTag User Guide
AccQFluor Reagent Kit*
Includes: AccQFluor Reagent 1, 5 x 6 mL vials, AccQFluor
Reagent 2A, 5 x 3 mg vials, AccQFluor Reagent 2B, 5 x 4 mL vials
AccQTag Column
3.9 x 150 mm
AccQTag Eluent A, Concentrate
1 x 1 liter
AccQTag Eluent B**
1 x 1 liter
Part No.
WAT052875
WAT088122
WAT052874
WAT052880
WAT052885
WAT052890
WAT052895
* The components of this kit are not available separately.

** For use with multi-pump gradient systems.
www.waters.com
Amino Acid Analysis
241
aMINO aCId aNaLYSIS
PicoTag HPLC Method
Lys
Leu
Reagent
Met SO2 (I.S.)
Ala
Pro
3 Nitro Tyr (I.S.)
Gln
Val
Plasma Amino Acid Profile Using the PicoTag Method
R
N = C = S + NH2
CH
S
COOH
NH
NH
CH
Phe
Orn
Ile
Cys
Met
1 Me His
NH3
Cit
Asn
His
Glu
PicoTag Derivatization Reaction
T hr
Ser
Arg
Tau
Trp
Tyr
Gly
A widely used technique for HPLC amino acid analysis is the Waters
PicoTag method. Based on an optimized system configuration, prepackaged reagents and extensive documentation, the PicoTag method provides
a turnkey, guaranteed approach to modern HPLC amino acid analysis.
Precolumn derivatization relies on the coupling reaction of the well known
Edman Degradation, the reaction of phenylisothiocyanate (PITC) with both
primary and secondary amino acids to form phenylthiocarbamyl (PTC) derivatives. The method is applicable to any sample including protein hydrolysates, physiologic fluids, feeds, foods, and pharmaceutical preparations.
COOH
66 Min.
The derivatization of amino acids with PITC is the first step of the wellknown Edman degradation reaction. The PTC-amino acid adducts are
stable and easily separated by reversed-phase HPLC. A single product is
formed for each amino acid. Most reaction by-products and all derivatization reagents are volatile, so they may be removed from the sample
by vacuum drying.
PicoTag Amino Acid Analysis of Protein Hydrolysates

Part No.
WAT007360
WAT088131
WAT088123
WAT088108
WAT088112
WAT088119
Phe
Cys
Ile
Leu
Dimensions/Qty.
PicoTag Chemistry Package (includes

column, reagent kit, eluents, diluent, manual,
and column heater inserts)
PicoTag Column
3.9 x 150 mm
Reagent Kit (contains PITC, TEA, and Standards)
PicoTag Eluent A
4 x 1 liter
PicoTag Eluent B
4 x 1 liter
PicoTag Diluent
100 mL bottle
PicoTag Amino Acid Analysis of Physiologic Amino Acids

Rgt
His
Ser
Gly
Arg
Asp
Glu
T hr
Ala
Met
Tyr
Pro
0.89
2.11
2.00
2.05
Val
Lys
A. Hydrolysate Standard
B. 16 Amino Acid Peptide, 40 pmol injected
T hr
Pro
Val
Lys
Courtesy of A.S. Feste, R.W. Drummond, and S.J. Dudrich, Nutritional Support Service,
St. Luke Episcopal Hospital, Houston, Texas.
Peptide Hydrolysate Amino Acid Analysis
Peptide
Composition
Asp
0.99
Glu
0.94
Gly
5.14
His
0.86
Reproducible and reliable plasma amino acid profiles are obtained in 66 minutes using Waters PicoTag Method.
In this analysis, 100 L plasma was diluted with an internal standard, deproteinized by centrifugal ultrafiltration,
and derivatized. The methionine sulfone (internal standard) peak represents 25 picomoles.
A
B
12 Min.
Free Amino Acid Chemistry Package (includes

column, reagent kit, eluents, diluent, manual,
column heater inserts, and sample tubes)
Free Amino Acid Analysis column
Reagent Kit (contains PITC, TEA and
Standards A/N and B)
PicoTag Eluent 1
PicoTag Eluent 2
PicoTag Diluent
Dimensions/Qty.
Part No.
WAT091681
3.9 x 300 mm
WAT010950
WAT010947
4 x 1 liter
4 x 1 liter
100 mL bottle
WAT010960
WAT010965
WAT088119
This 12 minute analysis using Waters PicoTag Amino Acid Analysis Method
provides identification and accurate quantitation of the amino acid composition.
242
Amino Acid Analysis
www.waters.com
aMINO aCId aNaLYSIS

MassTrak Amino Acid Analysis Solution Kit
T he MassTrak Amino Acid Analysis (AAA) Solution Kit forms an integral
part of an assured performance total system solution. Designed for use
with an ACQUITY UPLC system under Empower 2 software control and
configured with a TUV detector, the MassTrak AAA Solution Kit provides
a fully QC-tested column, pre-mixed eluents, vials and reagents for
the analysis of physiological amino acids in the researc h laboratory.
Standards are also provided for use with the application.
T he MassTrak AAA Solution Kit is intended for new installations of the
application and includes a system CD that delivers instrument, processing
and reporting methods as well as comprehensive user documentation.
Sample:
Upper: 25 pmole Physiological Amino Acid Standard

Lower: 100nL deproteinized plasma
MassTrak AAA, 2.1 x 150 mm
43 C
MassTrak Eluent A 1:10 Dilution
MassTrak Eluent B
0.4 mL/min
MassTrak Amino Acid Analysis Solution Gradient
(provided in the MassTrak Amino Acid Analysis Solution System Guide)
UV260
Column:
Temperature:
Eluent A:
Eluent B:
Flow Rate:
Gradient:
Detection:
T he MassTrak AAA Solution Kit includes:
AMQ
0.20
MassTrak AAA Reagent

- 6-aminoquinolyl-N- hydroxysuccinimdyl carbamate (AQC)
0.18
- Reacts rapidly with both primary and secondary amines

- Forms stable derivatives, permitting batch-wise processing
Trp
Phe
Ile
aIle
Leu
HCys
Nva
Met
Val
Tyr
Cys
GABA
AADA
Pro
BAIB
Hyl1
Hyl2
AABA
Ala
B-Ala
Thr
Glu
Cit
Derivatization Peak
Lys
Orn
0.10
Sar
AU
0.12
PSer
HyPro
NH3
His
Asn PEA
3MH
Tau
1MH
Ser
Gln
Carn
Arg
Ans Gly
EA
Asp
0.14
Cyst
0.16
- Prequalified components ensure reproducible high

resolution chromatographic separations
Pro
Trp
Phe
Leu
Ile
Tyr
Met
Glu
Cit
AABA
Thr
Orn
Val
Ala
Gly
Ser
Arg
HyPro
NH3
His
Asn
3MH
Tau
0.00
- Allows for quantification to 1 mol/L
Nva
Gln
0.04
0.02
Lys
Derivatization Peak
0.08
0.06
Quality-control tested columns, eluents, and reagents.
Full profile physiological amino acid standard, including glutamine,

allo-isoleucine, and norvaline (internal standard)
-0.02
4
10
12
14
16
18
20
22
24
26
28
30
32 min
The combination of Waters award-winning ACQUITY UPLC technology and market leading AccQTag
pre-column derivatization chemistry provides the robustness, reliability and ease of use required for
trouble-free operation in your research laboratory.
Description
Part No.
MassTrak Amino Acid Analysis Solution Application Kit*,

176001459
For Research Use Only
Includes:
MassTrak AAA Information Set providing methods for use with
the application and comprehensive system documentation.
150 mm Column Stabiliser
205000494
2 L Sample Loop
430001264
MassTrak AAA 2.1 x 150 mm Column
186004097
In-line Filter Kit
205000343
3 Total Recovery Vials with Screw Cap,
186000384C
non-preslit septa, 100 pack
MassTrak AAA Derivatization Kit
186004095
MassTrak AAA Standards Kit
186004099
MassTrak AAA Eluent A Concentrate
186004090
MassTrak AAA Eluent B
186004092
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
Description
Part No.
MassTrak Amino Acid Analysis Solution Kit**

For Research Use Only
Includes:
MassTrak AAA 2.1 x 150 mm Column
In-line Filter Kit
3 Total Recovery Vials with Screw Cap,
non-preslit septa, 100 pack
MassTrak AAA Derivatization Kit
MassTrak AAA Standards Kit
MassTrak AAA Eluent A Concentrate
MassTrak AAA Eluent B
176001498
186004097
205000343
186000384C
186004095
186004099
186004090
186004092
**For use after initial installation using MassTrak AAA Solution Application Kit 176001459.
Individual components of the MassTrak AAA Solution Kit are not available separately
* For use with new installations of the MassTrak AAA Solution. Individual components of the
MassTrak AAA Solution Application Kit are not available separately
www.waters.com
Amino Acid Analysis
243
OLIGONUCLEOTIdE SEPaRaTION TEChNOLOGY
Oligonucleotide Purification and Analysis

Oligonucleotides are employed in an ever-expanding host of applications,
including use as primers or hybridization probes, to adoption as therapeutic
agents. In brief, their synthesis is frequently performed via a series of stepwise reactions that result in the addition of specific nucleotides (protected
at their 5 hydroxyl end with a dimethoxytrityl group [DMT]) to the solid
phase support containing the growing chain. Variations in choice of incoming nucleotide monomers (both protected ribose and deoxyribose nucleotides), as well as modifications after synthesis, can yield a final product
containing atypical bases, sugars, or backbone composition. Some common
modifications include substitution of sulfur atoms or methyl groups for
oxygen on the phosphate backbone (creating phosphorothioates and methylphosphonates) or post-synthesis labeling to create molecular probes used
for various diagnostic investigations.
To keep pace with the ever increasing demand for high-quality oligonucleotides, automated synthesizers capable of producing 0.05 to
thousands of micromoles of product in a single synthesis exist. T hese
instruments and methods frequently possess high coupling-efficiency
capability wit h multi-strand synt hesis flexibility. Yet, even wit h
constant tec hnology advancements, the coupling efficiency in eac h
synthesis cycle is less than 100% resulting in contamination of the
target oligonucleotide product (e.g., 60 mers) with undesired impurities. T he total yield of full length product is a function of the length of
the desired sequence and the degree of synthesis coupling efficiency
as shown (right). Most failure products (typically labeled N-1, N-2...,
N-x) are shorter sequences generated by a premature coupling failure
of an incoming nucleotide monomer to the growing oligonucleotide
c hain. Some failures contain a missing nucleotide(s) in the middle
of sequence, rather than at the end. Mismatc hed failure sequences
having a greater molecular weight (often labeled N+x) than the desired
oligonucleotide product can also be produced from either incomplete
post-synthesis deprotection or from branc hing of the growing oligonucleotide c hain during synthesis.
Synthetic Oligonucleotide Length Compared to

T heoretical Yield at Various Coupling Efficiencies
100
90
80
70
% Overall Yield
Synthesis
60
99.5
50
40
99
.0
98
.5
98
.0
.5
30
97
20
97
.0
10
0
0
40
80
120
160
200
Length of Oligonucleotide
Isolation
A variety of methods exist for the lab-scale purification (25-500 nmole) of
oligonucleotides. T he advantages as well as disadvantages of techniques are
outlined in the table below.
Analysis
W hile polyacrylamide slab gel techniques can resolve long oligonucleotide
sequences, impurity detection of analyzed samples frequently involves
use of radio labeled nucleotides or gel staining techniques. T he advent of
capillary gel electrophoresis (CGE) with on-line detection helped overcome
some post-run detection issues. However, precise c hemical composition
determination of each separated oligonucleotide species cannot to determined using UV absorption detection. Furthermore, in some environments
the routine use of CGE for oligonucleotide analysis has been problematic due to method robustness and ruggedness concerns. HPLC, Waters
UltraPerformance LC Technology, and Mass Spectrometry have overcome
many challenges related to the separation, quantitation, and identification
of synthetic oligonucleotides. Rugged and robust separation and analysis
methods can frequently be performed in minutes rather than in hours. In
addition, quantitation with oligonucleotide composition confirmation is
possible using MS or hyphenated-MS methods.
Advantages
Disadvantages
Polyacrylamide Gel
Electrophoresis (PAGE)
Well-established and efficient method. It separates

long oligonucleotides (>50-60 mer).
Low mass-loading capacity. Gels are typically overloaded for purification and the resolution is compromised. PAGE does not separate N+x sequences. Manual band cutting. Excision is based on markers
without detailed knowledge of target oligo retention. Samples need to be extracted from the gel and
desalted, recovery of target oligonucleotides is low. Method is laborious; it is typically utilized only
when no other technique is suitable for the task.
Ion-Exchange Liquid
Chromatography (IEX-LC)
Trityl-off method. Separation of failure sequences is

due to the backbone charge.
IEX-LC is efficient only for relatively short oligos (<20-25 mers); longer oligos are poorly resolved.
Sample is contaminated with high concentration of salts; further desalting is required. IEX columns
packed with non-porous sorbent offer improved resolution, but suffer with low mass load capacity.
When loading exceeds 10-20 nmoles (for 4.6 mm i.d. columns), the resolution is compromised. IEX-LC
does not separate N+x sequences.
Trityl-on Liquid Chromatography

(Trytil-on LC; DMT-on LC)
Elegant, fast, and universal method for oligos of various length and
sequence. RP columns used with this method have sufficient mass
load capacity.
Does not adequately remove mismatch failure sequences (similarly as the target oligo they carry DMT
group). DMT group is labile, part of the product may be lost due to the spontaneous detritylation. DMT
residue and remaining acid have to removed after the detritylation.
Trityl-off Liquid Chromatography

(Trityl-off LC; DMT-off LC)
Effectively removes practically all types of failure products. Uses

volatile solvents; samples do not have to be further desalted.
Collected fractions are simply lyophilized and ready for use. RP
columns used with this method have sufficient mass load capacity.
Labeled and dually-labeled oligonucleotide probes can be also
purified. Method is suitable for LC-MS analysis (with MS compatible
ion-pairing buffers).
Method requires efficient columns packed with small particle size sorbent. Oligonucleotide retention
and resolution partially depends on the sequence. Method development for different oligonucleotide
sequence and length probes is necessary.
244
www.waters.com

Waters Oligonucleotide Separation Tec hnology (OST ) columns contain
second-generation hybrid silica BEH Tec hnology particles functionalized
with C18. T he separation of detritylated synthetic oligonucleotide samples
is based on t he well-establis hed met hod of ion-pair, reversed-phase
chromatography. T he availability of 1.7 m UPLC particles or 2.5 m HPLC
particles in various column dimensions provides flexibility to meet various
lab-scale isolation or analysis needs and delivers exceptional sample
resolution and superior column life. In addition, Waters manufacturing and
quality control testing procedures help ensure consistent batch-to-batch and
column-to-column performance regardless of application demands.
Separation efficiencies equivalent or better than

PAGE, CGE, or ion-exchange HPLC methods
Resolve failure sequences from detritylated full length products
Scaleable column offerings for lab-scale isolation needs
Exceptional column life for reduced cost per analysis
QC Tested with MassPREP
QC Tested with MassPREP OST

Standard to help ensure
performance consistency
Exceptional Resolution of
Oligonucleotide Mixtures
ACQUIT Y UPLC OST C18, 1.7 m (designed or use with an ACQUIT Y
UPLC System) and XBridge OST C18, 2.5 m columns are well suited for
the analysis of detritylated oligonucleotides using ion-pair, reversedphase c hromatography. As indicated (see figure on right), separations
are comparable to that obtained by capillary gel electrophoresis (CGE)
in terms of component resolution yet analyses times are significantly
decreased using Waters UPLC Tec hnology. T he ability to resolve large
oligonucleotide sequences (e.g., N from N-1) is possible due to the
enhanced resolving power obtained using sub-3 m, BEH Tec hnology
particles. In addition, quantitation with molecular weight c haracterization of the separated target oligonucleotide product from failure
sequences is possible using Waters OST columns with hyphenated-Mass
Spectrometry methods and MS friendly eluents.
Separation of Detritylated Oligodeoxythymidine Ladders by Capillary

Gel Electrophoresis (CGE) vs. Ion-Pair, Reversed-Phase Chromatography
System:
CGE Column:
Injection:
Running:
Temp:
Capillary Gel Electrophoresis System

PEG sieving matrix (BioCap 75 m x 27.5 (to detector) / 34.5 cm (total length)
45 injection at 5 kV
15 kV
30 C
10
15
UV 260 nm
12
LC system:
Column:
Mobile Phase:
Flow Rate:
Column Temp:
Gradient:
Detection:

ACQUITY OST C18, 1.7 m (2.1 x 50 mm)
A: 15 mM TEA, 400 mM HFIP, pH 7.9
B: 50% A, 50% MeOH
0.4 mL/min
60 C
40 to 48% B in 4 minutes
(20-24% MeOH)
260 nm
22 Min.
25
30
20
www.waters.com
30
35
4 Min.
245

Separation of a 15-60 mer Detritylated
Oligodeoxythymidine Ladder
LC System:

with PDA and QTof micro MS
ACQUITY UPLC OST C18,
1.7 m (2.1 x 50 mm)
A: 15 mM TEA - 400 mM HFIP,
B: Methanol
0.1 mL/min
Column:
Mobile Phase:
Flow Rate:
Impact of Different Ion-Pairing Agents on Varying

Oligonucleotide Sequence Separations
Column Temp.:
Gradient:
Detection:
60 C
19 to 26.5.5% B in 60 minutes
UV: 260 nm,
10 scans per second
MS: 1 scan per second,
0.1 sec interscan
9-11 %
ACN in 10 min
28-39 %
ACN in 10 min
UV 260 nm
15.5-23.5 %
MeoH in 10 min
23-37 %
ACN in 10 min
MS (TIC)
Improved Oligo separations can

be achieved using alternative
IP agents compared to use of
traditional TEAA
12-20 %
ACN in 10 min
45 Min.
UPLC/MS Analysis Of Interfering

RNA Oligonucleotides
10 Min.
Outstanding Column Life
Discovery of the RNA interference (RNAi) mechanism now broadly used

for silencing of target gene expression has prompted a need for the
analysis of small interfering RNAs (siRNA) molecules. To satisfy the
need for a robust, fast, and sensitive analysis of 20-25 nucleotides of
small interfering RNA (siRNA), a UPLC/MS method has been developed
utilizing UPLC OST columns and Synapt HDMS mass spectrometer.
T he acquisition of the accurate masses allowed for an assignment of the
peaks of 5-truncated oligomers (failed sequences generated during oligonucleotide synthesis), as well as some other impurities. T he mass of each
peak in the MS chromatogram was deconvoluted using MaxEnt 1 software.
T he tentative 5-end failure products are assigned in Figure 2. Nearly the
entire sequence of the parent oligonucleotide was elucidated. MS analysis
also revealed a presence of an extra uridine mononucleotide added to the
target 21-mer RNAi sequence.
Waters OST columns packed with BEH Tec hnology particles have shown
remarkable column longevity, under these demanding separation conditions, while maintaining outstanding separation performance. By comparison, significantly reduced column life results when traditional silica-based
columns are used under these same demanding separation conditions.
Separation of 5-25 mer Detritylated Oligodeoxythymidine Ladder
HPLC System:
Column:
Mobile Phase:
Column temp:
Gradient:
Flow Rate:
Detection:
Alliance Bio 2796 with PDA

XBridge OST C18, 2.5 m (2.1 x 50 mm)
A: 10% MeOH / 90%
(385mM HFIP + 14.3mM TEA)
B: 25% meOH / 75%
(385mM HFIP + 14.3mM TEA)
60 C
0 100% B in 30 min
(10-25 % MeOH).
1.0 mL/min
260 nm,
5 scans per second
Injection #4
Injection #1001
LC/MS Analysis of RNA (21 mer)

Synapt MS
2000 V
35 V
3V
200 C
120 C
50 L/ hr
600 L/ hr
LC System:
Column:
Column Temp.:
Sample Injected:
Flow Rate:
Mobile Phase A:
Mobile Phase B:
Gradient:
Detection:
TEA
20 nt
8.73 min
Waters ACQUITY UPLC

ACQUITY UPLC OST C18, 2.1 x 50 mm, 1.7 m
60 C
2.5 L (100 pmole of 21 nt RNAi)
0.2 mL/min
15 mM TEA. 400 mM HFIP
50% A, 50% methanol
20-40% B in 10 min.
RNAi
PDA, 260 nm UV
21-mer
TEA
TIC MS:
Capillary:
Sample Cone:
Extraction Cone:
Desolvation Temp.:
Source Temp.:
Cone Gas Flow:
U
5800
C U
680 0
m/z
U G
U
U
10 Min.
Description
ACQUITY UPLC OST C18 *
Custom ACQUITY UPLC OST C18 *
ACQUITY OST C18
Method Validation Kit**
XBridge OST C18
XBridge OST C18
XBridge OST C18
Custom XBridge OST C18
XBridge OST C18
Method Validation Kit**
Particle
Size
1.7 m
1.7 m
1.7 m
1.7 m
2.5 m
2.5 m
2.5 m
2.5 m
12
15
18
21
Pore
Size
Dimension
135 2.1 x 50 mm
135 2.1 x 100 mm
135 2.1 x 150 mm
24
27
30 Min.
Part No.
186003949
186003950
186003951
186005516
2.1x 100 mm
186004898
135 2.1 x 50 mm
135 4.6 x 50 mm
135 10 x 50 mm
186003952
186003953
186003954
186003955
4.6 x 50 mm
186004906
* For use on Waters ACQUITY UPLC Systems

**Three columns from three different batches of material.
246
www.waters.com

Scalable DNA and RNAi Separations
with Good Product Recovery
Purification of siRNA Duplex from Impurities

LC System:
Column:
XBridge OST C18 columns are the preferred offering for detritylated oligonucleotide purifications due to the availability of column sizes designed
to meet lab-scale isolation requirements. As indicated in the table below,
the c hoice of XBridge OST C 18 column dimension and operating flow
rate depends primarily on the scale of the synthesis reaction mixture.
For example, a 4.6 x 50 mm column containing XBridge OST C 18,
2.5 m material is an excellent selection when oligonucleotide mass
loads are less than or equal to 0.2 mol. Selection of the appropriate
column size for the amount of oligonucletide sample loaded is recommended to maximize component resolution and recovery of the target
product from non-desired failure sequences.
Column Temp:
Flow Rate:
Mobile Phase A:
Mobile Phase B:
Gradient:
Detection:
Target siRNA
duplex
85 nmol
RNA
Impurities
15 nmol
siRNA
Impurities
1.5 nmol
0.15 nmol
For researchers involved in gene silencing it is often necessary to work

with RNA of high purity. Crude synthetic oligonucleotides used for gene
knockout are typically purified. T he figure below illustrates a a lab-scale
purification of 21 mer RNA at various column loads. Using OST column
chemistry and a Alliance System, large quantities of crude single stranded
RNA can be successfully purified yielding material of high purity, ca. 95%,
with an estimated yield of 55% based on collected peak area to the total
peak area of the sample.
In addition, OST columns are well suited for analysis and purification of
siRNA. As shown in the figure to the right siRNA is well resolved from
single stranded RNA and truncated duplexes.
Waters Alliance Bio

XBridge OST BEH C18
4.6 x 50 mm, 2.5 m
20 C
1.0 mL/min
0.1 M TEAA, pH 7.0
20% ACN in A
25 - 75% B in 30.0 min
PDA, 260 nm
25 Min.
Dimensions
2.1 x 50 mm
4.6 x 50 mm
10 x 50 mm
19 x 50 mm*
30 x 50 mm*
50 x 50 mm*
*
Approx Mass Load**
mg***
Flow Rate
0.04 moles
0.20 moles
1.00 moles
4.00 moles
9.00 moles
25.00 moles
0.2 mg
1.0 mg
4.5 mg
16.0 mg
40.0 mg
110.0 mg
0.2 mL/min
1.0 mL/min
4.5 mL/min
16.0 mL/min
40.0 mL/min
110.0 mL/min
OST Custom Column
** Values are only approximates and vary depending on oligonucleotide length, base composition,
and heart-cutting fraction collection method used.
*** Estimated for average oligonucleotide MW and synthesis yield.
Purification of Single Stranded RNA

LC System:
Column:
Column Temp:
Flow Rate:
Mobile Phase A:
Mobile Phase B:
Gradient:
Detection:
Waters Alliance Bio

XBridge OST BEH C18, 4.6 x 50 mm, 2.5 m
60 C
1.0 mL/min
0.1M TEAA, pH 7.5
20% Acetonitrile in A
30 52.5% B in 10.0 min (0.15% ACN/min)
PDA, 260 nm
Literature
References
Purified Oligonucleotide
Purity > 95%
140 nmol
N-1
Purity 78%
*
0
84 nmol
97.8% UPLC purity (area)
UPLC/MS Analysis of Interfering RNA

Oligonucleotides Application Note,
Optimization of LCT Premier XE MS Settings

for Oligonucleotide Analysis Application Note,
UPLC Analysis of Phosphorothioate

Oligonucleotides: Method
Development Application Note,
UPLC Separation of DNA

Duplexes Application Note,
28 nmol
UPLC/UV-MS Analysis of Phosphorothioate

1.4 nmol
0
RNAi Duplex Analysis and

Purification Application Note,
18 Min.
Semi-Preparative Scale Single-Stranded RNA

Purification Application Note,
Real-Time Analysis of RNAi
Duplexes Application Note,
UPLC/UV-MS Analysis of
Application Note Oligonucleotides,
www.waters.com
UPLC/MS Separation of Oligonucleotides

in Less than Five Minutes: Method
Oligonucleotide Separation Technology:
Synthesis Challenges and HPLC Isolation
Options Application Note,
UPLC Separation of Oligonucleotides: Method
HPLC and UPLC Columns for the Analysis of
247

Columns for Large DNA/RNA Species
In general, molecular biology methods for manipulation of DNA rely on
restriction enzymes, polymerase-c hain reaction (PCR), and sequencing
techniques. Using these methods, genomic DNA is typically converted into
shorter double stranded (ds)DNA sequences, typically 100-1000 base
pairs (bp) in length. T he shorter dsDNA molecules are often analyzed or
isolated by methods suc h as slab gel or capillary electrophoresis. Use
of Waters ACQUITY BEH300 C18 reversed-phase or GenPak FAX anionexchange columns offer alternatives to more traditional electrophoretic
methods and are particularly well suited for various analytical and smallscale purification applications.
Description
Dimension
Particle Size
Part No.
2.1 x 50 mm
1.7 m
186003685
Separation of Duplex DNA Fragments: HaeIII and MspI

Restriction Enzyme Digests of pBR322 Plasmid
LC System:
Column:
Column Temp:
Flow Rate:
Mobile Phase A:
Mobile Phase B:
Gradient:
Detection:
Waters ACQUITY
ACQUITY UPLC PST BEH300 C18 , 2.1 x 50 mm, 1.7m
50 C
0.2 mL/min
0.1M TEAA, pH 7.0
20% ACN in A
57.5 84.5 % B in 20.0 min
PDA, 260 nm
20 Min.
Gen-Pak FAX Anion-Exchange Columns

Waters Gen-Pak FAX columns offer the highest resolution available in
anion-exchange HPLC of nucleic acids. T he Gen-Pak FAX column contains
a weak anion exchanger based on DEAE functionalized non-porous resin.
It contains 2.5 m particles and is well suited for analytical and micropreparative applications.
Chromatography of a PCR Amplification Mixture Generated
using 3 ng and 1 fg of HBV S-gene Template
Sample:
Column:
Eluent A:
Eluent B:
PCR amplification mixture using 3 ng HBV-S gene template

Gen-Pak FAX (4.6 x 100 mm)
25 mM Tris/Cl, 1 mM EDTA, pH 8.0
25 mM Tris/Cl, 1 mM ESTA, 1.0M NaCl, pH 8.0
dNTPs and Taq enzyme
Gradient:
Flow Rate:
Temperature:
Separation of DNA Restriction Fragments

Sample:
Column:
Eluent A:
Eluent B:
Gradient:
Flow rate:
Temperature:
Detection:
3.0 g of BstN1 digest of pBR322

Gen-Pak FAX, 4.6 x 100 mm column
25 mM Tris/Cl, 1 mM EDTA,
pH 8.0
25 mM Tris/Cl, 1 mM EDTA,
1.0 M NaCl, pH 8.0
25-75% B in 30 min, linear
0.75 mL/min
30 C
260 nm
40 - 75% B in 30 min., Linear

0.75 mL/min.
30 C
1. 13 bp
2. 121 bp
3. 383 bp
4. 929 bp
5. 1060 bp
6. 1857 bp
45
3
2
1
35 Min.
465 bp PCR product
Absorbance 260 nm
0.040
Nonspecific DNA sequences
Description
0.000
0
248
15
30 Min.
Gen-Pak FAX Column
Dimensions
Part No.
4.6 x 100 mm
WAT015490
www.waters.com
OligOnucleOtide SeparatiOn technOlOgy

MassPREP OST Standard
Contains a carefully defined mixture of synthesized
15 nt
oligodeoxythymidine fragments
20 nt
Useful in testing and confirming HPLC/UPLC, LC/MS,
25 nt
and column performance for oligo applications
30 nt
35 nt
Each QC tested and shipped with a certificate of analysis
T he pre-packaged MassPREP Oligonucleotide Separation Technology (OST)

Standard is designed for verification of HPLC/UPLC instrument and column
performance for analysis of synthetic oligonucleotides. Approximately
equimolar amounts of 15, 20, 25, 30, and 35 nucleo-tide (nt) long
oligodeoxythymidines are lyophilized and packaged in 1.5 mL LC vials.
T hese vials are vacuum-sealed in foil pouches to reduce degradation that
can occur by excessive exposure to light and air. Approximately 1 nmole of
each oligonucleotide is present in the vial.
Description
Qty.
Part No.
1/pk
186004135
10 Min.
Waters ACQUITY UPLC analysis of MassPREP OST Standard on an ACQUITY UPLC OST C18, 1.7 m column.
The main components are labeled. Small peaks eluting between labeled oligonucleotides are N-1, N-2, etc.
failure sequences generated during the oligonucleotide syntheses. The ACQUITY UPLC system is equipped
with 50 L standard mixer and PDA detector (260 nm).
Oasis Elution Plates

Effective Use of Oassis HLB for Oligonucleotide Desalting Prior to MALDI-TOF MS
Oligonucleotide Desalting by Solid-Phase Extraction

Removes salt prior
to MS analysis
Low elution volumes
High sensitivity
Sample concentrating
MALDI-TOF Spectrum of 16 mer Prior to Desalting
High throughput
900
800
Matrix Solution:
700
Mode:
Abundance
600
70 mg/mL 3-hydroxypicolinic acid

and 40 mM Ammonium Acetate
Negative Ion
500
400
300
200
100
0
4000
4500
5000
5500
M/Z
MALDI-TOF Spectrum of 16 mer After Desalting

using Oasis HLB Elution Plate
1800
Matrix Solution:
1600
Mode:
1400
70 mg/mL 3-hydroxypicolinic acid

and 0.04 M Ammonium Acetate
Negative Ion
1200
Abundance
Desalting of synthetic oligonucleotides is essential for MS analysis (QC,

genotyping applications and SNP analysis). Waters new Elution plate is
an excellent choice for high throughput analysis with minimal amount of
sample. T he Oasis Elution plate combines patented plate design, proven
Oasis c hemistries, and generic protocols enabling elution volumes
as low as 25 L. Now for the first time you can perform SPE cleanup and concentration of very small sample volumes. T he Oasis HLB
sample extraction products incorporate a patented copolymer made
from a balanced ratio of two monomers; the lipophilic divinylbenzene and
the hydrophilic N-vinylpyrolodone that is ideally suited for this application.
1000
800
600
400
Description
Part No.
200
0
4000
Oasis HLB Elution Plate (for oligonucleotides)
www.waters.com
186001828BA
4500
5000
5500
M/Z
249
GLYCaN aNaLYSIS
Glycan Separation Technology

Waters ACQUITY UPLC BEH Glycan column was designed for the HILICmode separation of 2-aminobenzamide (2-AB) labeled glycans. T his
column c hemistry and recommended UPLC instrument conditions are
capable of separating both neutral and c harged species using a binary
gradient. Retention of 2-AB- labeled oligosacc harides is based on the
hydrophilicity of the molecule. T he high resolving power of this column
is due in part to the small particle size, 1.7 m, of the fully porous
packing material. Chemical and mec hanical stability of the column are
the consequence of Waters bridged-ethyl hybrid (BEH) particle composition and ligand bindingTec hnology and help ensure consistent batc h-tobatc h performance.
Improved component resolution in less time compared

to existing HPLC-based methods
Optimized for use with ACQUIT Y UPLC system with

fluorescence detection
Based on Waters BEH particle and bonding tec hnology

for stable and reproducible labeled glycan separations
Quality-control tested with relevant labeled glycan standards

for consistent batc h-to-batc h reproducibility
ACQUITY UPLC BEH Glycan Column Separation of 2-AB Labeled Human IgG Glycans
UPLC Conditions
Sample:
Sample Volume:
Injection Mode:
Column:
Eluent A:
Eluent B:
Temperature:
Detection:
2-AB-labeled IgG derived glycans

10 pmol/L
1.5 L
Partial Loop
ACQUITY UPLC BEH Glycan, 2.1 x 150 mm
100 mM Ammonium Formate, pH 4.5
Acetonitrile
60 C
Fluorescence: ex = 330 nm, em = 420 nm
Gradient:
Instrument Conf.:
Time
Flow rate
(min)
(mL/min) % A
%B
Init
0.5
25
75
46.5
0.5
40
60
48
0.25
100
0
49
0.25
100
0
50
0.5
25
75
63
0.5
25
75
Mixer (Part No. 205000403).
Needle (Part No. 205000507).
No in-line filter. No VanGuard column.
1. 2AB
Curve
6
6
6
6
6
6
6,7. 2AB
2. 2AB
8. 2AB
13. 2AB
3. 2AB
9. 2AB
4. 2AB
12. 2AB
10. 2AB
5. 2AB
11. 2AB
N-Acetylglucosamine
Fucose
Manose
Sialic Acid
Galactose
10
The N-linked glycans from pooled human IgG contain high mannose as
well as neutral, and sialylated complex structures. The chromatogram
shows 35 minutes of a one hour analysis. This challenging sample
includes high mannose, bisecting GlcNAc and sialylated species.
13
3
4
7
5
8
9
10
250
15
Glycan Analysis
20
12
11
25
30
35 min
www.waters.com
GLYCaN aNaLYSIS
High Quality Separations of Varied Glycan Structures
glycan. Waters ACQUITY UPLC BEH Glycan column was specifically
designed for use on Waters ACQUITY UPLC System to give improved
component resolution compared to traditional LC techniques for more
certain identification and reliable quantitation.
Several complementary analytical techniques are routinely used to characterize, identify, and quantitate oligosaccharides isolated from glycoproteins.
Hydrophilic-interaction liquid chromatography (HILIC) is a well-recognized
and reliable technique that can effectively separate fluorescently-labeled
ACQUITY UPLC BEH Glycan Column Separation of 2AB-Labeled Dextran Ladder

UPLC Conditions
Sample:
Sample Volume:
Injection Mode:
Column:
Eluent A:
Eluent B:
Temperature:
Detection:
Gradient:
2-AB-labeled Dextran Ladder in Glucose Units

10 pmol/L
1.5 L
Partial Loop
100 mM Ammonium Formate, pH 4.5
Acetonitrile
60 C
Time
Flow rate
(min)
(mL/min) % A
%B
Init
0.5
25
75
46.5
0.5
50
50
48
0.25
100
0
49
0.25
100
0
50
0.5
25
75
63
0.5
25
75
Numbers on peaks are GU units,

not compounds on key (previous page).
1
6
2
10
11
12
13
14
15
16
17
18
19
20
Curve
6
6
6
6
6
6
10
15
20
25
30
35
40
21
22
45 min
The fast, high-resolution separation of a homopolymeric series of glucose oligomers illustrates the wide range of glycan sizes, extending beyond 22 glucose units that can be separated with the
ACQUITY UPLC BEH Glycan column. These labeled glycans can provide calibration reference points that are helpful in identifying more complex glycans based on relative retention times.
ACQUITY UPLC BEH Glycan Column Separation of 2AB-Labeled High Mannose Glycans from Ribonuclease b
UPLC Conditions
Sample:
Sample Volume:
Injection Mode:
Column:
Eluent A:
Eluent B:
Temperature:
Detection:
Gradient:
2-AB-labeled ribonuclease b N-glycans

100 pmol/L
1.5 L
Partial loop
100 mM ammonium formate, pH 4.5
Acetonitrile
60 C
Time
Flow rate
(min)
(mL/min) % A
%B
Init
0.5
25
75
46.5
0.5
40
60
48
0.25
100
0
49
0.25
100
0
63
0.5
25
75
1. Mannose 5
2AB
4. Mannose 8
2AB
2. Mannose 6
2AB
5. Mannose 9
2AB
Mannose 7
isomers
2AB
3a, 3b.
2*
Curve
6
6
6
6
6
4*
3*
5
10
15
20
25
30
35 min
A series of high mannose oligosaccharides was released from bovine ribonuclease b and labeled with 2-AB. The HILIC separation
performed on ACQUITY UPLC Glycan column shows both different numbers of mannose residues and isomeric structures.
ACQUITY UPLC BEH Glycan Columns

Column Description
ACQUITY UPLC BEH Glycan, 1.7 m, 2.1 x 50 mm
ACQUITY UPLC BEH Glycan, 1.7 m, VanGuard Pre-column
Method Validation Kit*
Part No.
186004740
186004741
186004742
186004739
Note: ACQUITY UPLC BEH Glycan, 1.7 m columns are designed for use with the
ACQUITY UPLC system. The benefits of the small particle packing in ACQUITY UPLC
BEH Glycan, 1.7 m columns are only realized with the low system volume and low
detector dispersion of an ACQUITY UPLC system.
186004907
*Three columns from three different batches of material.
www.waters.com
Glycan Analysis
251
Glycan analysis
MassPREP Glycoanalysis Kit
Sample Preparation from Glycoprotein

to Purified 2-AB Labeled Glycans
Glycoproteins
Optimize protein deglycosylation reactions with

RapiGest SF surfactant
Minimize sample manipulations
Effective desalting /sample clean-up method
Compatible with MALDI MS and other glycan analysis techniques
Assist in isolation of 2-AB labeled glycans
Denaturation with MassPREP RapiGest

DTT Reduction (if necessary),
Deglycolisation by PNGase F
Protein Removal
MassPREP HILIC Elution Plate
2-AB Labelling of Purified Glycans
Excess Labelling Agent Removal

Purified 2-AB Labeled Glycans
MALDI MS
T he MassPREP Glycoanalysis kit provides simple and robust sample preparation without compromising sample recovery. As shown (right), unlabeled
or 2-AB labeled glycans can be successully analyzed by MALDI-MS or by
LC with fluorescence detection following MassPREP Glycoanalysis Kit use.
MALDI MS Mass Profiling of Native and 2AB-Labeled IgG Glycans

N-Acetylglucosamine
Fucose
Manose
Galactose
A)
(M+Na)
m/ z
G0
1339.47
G0F
1485.53
G1F
1647.58
G2F
1809.63
G1F
10 0
G0 F
Glycosylation is one of the most important types of post-translational

modification (PTM) in eukaryotic proteins. Efficient sample deglycosylation
and sample preparation is a key requirement for successful and sensitive
glycan analysis. In addition, the preparation and purification 2-AB-labeled
glycans released from glycoproteins can also be an important step in their
successful analyses.
LC-FLR
Underivatized glycans after

protein removed by HILIC plate
G2F
G0
0
B)
Description
Dimensions/Qty.
MassPREP Glycoanalysis Kit

(includes, MassPREP HILIC Elution Plate,
RapiGest SF, and MassPREP MALDI Matrix DHB)
Part No.
2AB - G0F
10 0
2AB - G1F
186002817
2AB - G2 F
2AB - G0

RapiGest SF
96-well
1 mg vial
186002780
186001860
For more information see RapiGest SF Page 257
252
Glycan Analysis
Labeled glycans (+120.1 Da)

after excess labeling reagents
removed by HILIC plate
0
1200
1400
1600
1800
2000
2200
2400
2600
2800
m /z
MALDI-TOF MS spectra of: (A) 8 pmol of the unlabeled IgG1glycans after first HILIC cleanup prior
to labeling. (B) 3 pmol of the 2-AB-labeled IgG1 glycans after second HILIC cleanup.
Each spectrum shows well purified glycans with no inferences from impurities.
www.waters.com
MS aNd LC/MS CONSUMabLES FOR bIOMOLECULES

The use of mass spectrometry is increasing in laboratories involved with
proteomics and the characterization of biopharmaceuticals. Sample preparation, the testing of system performance with simple standards, the use high
quality reagents and matrices, and the LC process are critical to the quality
of results.
Only when all these elements are considered can the highest quality results
can be obtained. Waters has developed MS and LC/MS consumables specifically designed to ensure the best results from MS and LC/MS systems.
LC/MS
Proteomics
The MassPREP family of products includes conveniently packaged,

highly-purified standards, specialty plates, and kits and reagents.
Protein
Characterization/QC
RapiGest SF
RapiGest SF
MassPREP Protein Digestion Standards
MassPREP Digestion Standards Mixtures
MassPREP Peptide Reference Standards
MassPREP Peptide Reference Standards
MassPREP Phosphopeptide Standards
MassPREP Desalting Cartridges
NanoEase Traps, Capillary, and Nano Columns
NanoEase Traps, Capillary, and Nano Columns
MassPREP Phosphopeptide
Enrichment Kit
nanoACQUITY UPLC Traps, Capillary,

and Nano Column and 2D Kit
nanoACQUITY UPLC Traps, Capillary,

and Nano Column and 2D Kit
The NanoEase family contains high performance

capillary, nano-scale, and trapping columns.
nanoACQUITY UPLC columns consist of a complete line of columns

specifically designed for the nanoACQUITY UPLC system.
www.waters.com
253

RapiGest SF and MassPREP Oligonucleotide,
Protein, Peptide, and Protein Digest Standards
Prepackaged MassPREP synthetic oligonucleotide peptide and protein digest
standards eliminate the need to prepare, test, and store these materials
for use in LC or LC/MS applications. In addition, RapiGest SF Surfactant is
valuable in the enzymatic digestion of proteins for faster, higher quality,
and more reproducible sample preparation.
MassPREP Peptide Standards
* BioSuite C18 3 m, Peptide Analysis - A Column (2.1 x 150 mm). Eluent A: 0.02% TFA
in water, Eluent B: 0.016% TFA in acetonitrile. Gradient: 0-50% B in 30 minutes. Flow:
0.20 mL/min. Temp: 40 C.
** Monoisotopic molecular weight
254
MassPREP Standards and RapiGest
Enolase T37
Enolase T35
Melittin
Peptide sequence
RGDSPASSKP
DRVYIHP
RPPGFSPFR
DRVYIHPF
DRVYIHPFHL
DRVYIHPFHLLVYS
WLTGPQLADLYHSLMK
YPIVSIEDPFAEDDWEAWSHFFK
GIGAVLKVLTTGLPALISWIKRKRQQ
RASG-1
pKa
9.34
7.35
12.00
7.35
7.51
7.61
7.34
3.97
12.06
Allantoin
Molecular weight **
(g/mol)
158.0440
1000.4938
898.4661
1059.5613
1045.5345
1295.6775
1757.9253
1871.9604
2827.2806
2845.7381
AU
Component
Peak # name
1 Allantoin (V0 marker)
2 RASG-1
3 Angiotensin frag.1-7
4 Bradykinin
5 Angiotensin II.
6 Angiotensin I.
7 Renin substrate
8 Enolase T35
9 Enolase T37
10 Melittin
Renin substrate
0.5
Components contained in MassPREP Peptide Standard Mixture
Bradykinin
Angiotensin II
Angiotensin I
Baseline HPLC Resolution of Nine Peptides

Contained in MassPREP Standard Mixture
Angiotensin frag. 1-7
T he MassPREP peptide standard mixture contains a void volume (V O)

column marker and nine carefully selected peptides with a broad range of
polarities and isoelectric points. T he MassPREP standard is useful to test
UPLC and HPLC columns and systems dedicated to peptide separations.
0.0
0
10
20
30 Min.
Chromatography of MassPREP Peptide Standard Mixture obtained on a BioSuite C18,

3 m Peptide Analysis A Column (2.1 x 150 mm) performed on a Waters Alliance HPLC System.
Description
Qty./Pack
Part No.
1 vial
5 vials
186002337
186002338
Note: The peptide mixture contains approximately 1.5 g (~1 nmole) of each peptide
www.waters.com

T he MassPREP protein digestion sandards are prepared under strict quality
control and contain no undigested standard proteins, trypsin, or other
hydrophilic components. Test results from each batch of digestion standards
are provided on the included Certificate of Analysis report.
Description
Qty./Pack
Part No.
MassPREP Digestion Standard Kit

1 vial from each digestion
Standard (5 vials total) Kit Consists of:
MassPREP Phosphorylase b Digestion Standard
1 vial
MassPREP Bovine Hemoglobin Digestion Standard 1 vial
MassPREP ADH Digestion Standard
1 vial
MassPREP BSA Digestion Standard
1 vial
MassPREP Enolase Digestion Standard
1 vial
186002330
186002326
186002327
186002328
186002329
186002325
MassPREP Protein Standard Mix

T he MassPREP Protein Standard is a carefully chosen mixture of a variety
of proteins encompassing a wide range of properties. T his mixture contains
proteins that vary in isoelectric points, molecular weights and hydrophobicities. T hese characteristics provide the user with an attractive intact protein
validation mixture that can be used for a variety of applications.
Description
Qty./Pack
Part No.
1 vial
186004900
MassPREP Protein Standard
MassPREP Protein Standard Mix

Protein Sample
Molecular Weight, MW
Isoelectric Point, pI
Ribonulcease A, bovine pancrease
13.7 k
9.6
Cytochrome c, horse heart, 96%
12.4 k
10.25
Albumin, bovine serum, 96-99%
66.4k
5.8
Myoglobin, horse heart >90%
16.7 k
6.53
Enolase from bakers yeast (S. cereviasiae)
46.7 k
6.53
97 k
7.18
Phosphorylase b, rabbit muscle
T he MassPREP phosphopeptide standard-enolase contains four phosphopeptides based on tryptic yeast enolase peptides. T he MassPREP enolase digest
with phosphopeptide mix contains equimolar mixture of enolase digest and
four phoshopeptides.
Contains phosphoserince, phosphotyrosine,

and phosphothreonine peptides
Use to optimize phosphopeptide detection in

LC/MS, LC/UV, and MALDI-MS
MALDI MS
Can be used as control samples
T18 1P
MassPREP Phosphopeptide Standard - Enolase
Phosphorylated protein and peptides are difficultT43

to 1P
detect and characterize due to their
low
abundance
and
low
ionization
efficiency.
Using these
T19 1P
T43 2P
standards, scientists have greater control over sample preparation, with the
option to use pure peptides or to define phosphopeptides to unmodified
peptide ratios.
800
900
1000
1100
T18 1P
T43 2P
T19 1P
15
T19 1P
w/enolase
peptides
10
1300
1400
1500
m/z
1600
MassPREP Phosphopeptide Standard - Enolase
LC MS
10
1200
T18 1P
15
www.waters.com
20
Synthetic
Phosphopeptides
Sequence
Amino Acid
Residue
[M + H]+
[M + 2H]2+
Enolase T18 1P
NV PL(pY) K
126-131
813.3912
407.1995
Enolase T19 1P
HLADL (pS)K
132-138
863.4028
432.2053
Enolase T43 1P
VNQIG (pT)LSES IK
346-357
1368.6776
684.8428
Enolase T43 2P
VNQIG TL(pS)E(pS) IK
346-357
1448.6439
724.8259
T hese standards can be used on many Waters instrument systems, including the MALDI micro
MX, ACQUITY UPLC, Alliance , ZQ, Q-Tof, LCT Premier, and nanoACQUITY UPLC systems. Either
UV or MS detection is possible.
T43 1P
25
Each new phosphopeptide standard contains

the following four purified peptides:
30
35
40
45 Min.
MassPREP Enolase Digest with Phosphopeptides Mix
Description
T43 2P
MassPREP Phosphopeptide Standard Enolase

MassPREP Enolase Digest with Phosphopeptides Mix
MassPREP Phosphopeptide Sample Kit - Enolase
2 vials Kit Consists of:
MassPREP Phosphopeptide Standard
Enolase
1 nmol/vial, 1 vial
1 vial
20
T43 1P w/enolase peptides
25
30
35
40
45 Min.
Qty./Pack
Part No.
186003285
186003286
186003287
186003285
186002325
255
MS and LC/MS ConSuMabLeS for bioMoLeCuLeS

MassPREP Digestion Standard Mixtures
Used to ensure an analytical systems ability to provide both

qualitative protein analysis and relative protein quantification
Yields high confidence and coverage during expression profiling

of complex protein samples (by spiking the standards)
Designed as standards for the Waters Protein Expression System
MPDS Mix 1 and MPDS Mix 2 Separation Using ACQUITY UPLC BEH
C18, 1.7 m, 75 m x 100 mm nanoACQUITY UPLC Column
MassPREP Digestion Standards consist of two standard mixtures (MPDS

Mix 1 and MPDS Mix 2), which were prepared by individually digesting
Yeast Alcohol Dehydrogenase (ADH, SwissProt P00330), Rabbit Glycogen
Phosphorylase b (GPB, SwissProt P00489), Bovine Serum Albumin (BSA,
SwissProt P02769), and Yeast Enolase I (ENO, SwissProt P00924) with
sequencing grade trypsin, and producing mixtures in the indicated molar
ratios. The MPDS mixtures are purified, and do not contain undigested
target protein, trypsin, other hydrophilic components or salts.
MPDS Mix 2
MPDS Mix 1
MPDS Mix 1 and Mix 2 Contents

MPDS Mix 1
[Molar ratio, Amount (pmol)]
MPDS Mix 2
[Molar ratio, Amount (pmol)]
ADH
1.0, 50 pmol
1.0, 50 pmol
GPB
1.0, 50 pmol
0.5, 25 pmol
ENO
1.0, 50 pmol
2.0, 100 pmol
BSA
1.0, 50 pmol
8.0, 400 pmol
Protein
Sample:
MPDS Mix 1: Alcohol Dehydrogenase
25 fmol/L, PhosphorylaseB 25 fmol/
L; Enolase 25 fmol/L; Bovine Serum
Albumin 25 fmol/L MPDS Mix 2: Alcohol
Dehydrogenase 25 fmol/L; Phosphorylase
B 12.5 fmol/L; Enolase 50 fmol/L;
Bovine Serum Albumin 200 fmol/L
Column:
Mobile Phase A:
Mobile Phase B:
Gradient:
Injection Volume:
Detection:
Ionization:
Mode:
nanoACQUITY UPLC BEH C18,

1.7 m, 75 m x 100 mm
0.1% FA in H2O
0.1% FA in ACN
Linear Gradient from 3 to 40% B in 90 min.
2 L
MS on Q-Tof Premier (m/z 50-1990)
ESI
Positive
Description
Qty.
Part No.
MassPREP Digestion Standard Mix 1

MassPREP Digestion Standard Mix 2
1/pk
1/pk
186002865
186002866
Contains a carefully defined mixture of synthesized

oligodeoxythymidine fragments
15 nt
20 nt
Useful in testing and confirming HPLC/UPLC, LC/MS,

and column performance for oligo applications
25 nt
35 nt
Each QC tested and shipped with a certificate of analysis
T he pre-packaged MassPREP Oligonucleotide Separation Technology (OST)

Standard is designed for verification of HPLC/UPLC instrument and column
performance for analysis of synthetic oligonucleotides. Approximately
equimolar amounts of 15, 20, 25, 30, and 35 nucleotide (nt) long
oligodeoxythymidines are lyophilized and packaged in 1.5 mL LC vials.
T hese vials are vacuum-sealed in foil pouches to reduce degradation that
can occur by excessive exposure to light and air. Approximately 1 nmole of
each oligonucleotide is present in the vial.
Description
256
30 nt
Qty./Pack
Part No.
1 vial
186004135
10 Min.
Waters ACQUITY UPLC analysis of MassPREP OST Standard on an ACQUITY UPLC OST C181.7 m column.
The main components are labeled. Small peaks eluting between labeled oligonucleotides are N-1, N-2, etc.
failure sequences generated during the oligonucleotide syntheses. The ACQUITY UPLC system is equipped
with 50 L standard mixer and PDA detector (260 nm).
www.waters.com

RapiGest SF Protein Digestion Surfactant
Waters RapiGest SF* (surfactant) radically enhances protein enzymatic
digestions in terms of speed and percent recovery. RapiGest is a
patented anionic surfactant that accelerates the production of peptides
generated by proteases, suc h as trypsin, Asp-N, Glu-C, and Lys-C. Many
hydrophobic proteins are resistant to proteolysis because their cleavage
sites are inaccessible to endoproteases. RapiGest, a mild denaturant,
helps solubilize and unfold proteins making them more amenable to
cleavage without denaturing or inhibiting common proteolytic enzymes.
Improves solubility of hydrophobic proteins for

improved enzymatic digest
1 hour Proteolysis of Myoglobin Using Various Endoproteases

B) Control
A) 0.1% (w/v) RapiGest
Intact Myoglobin
Asp-N
Lys-C
Glu-C
Compatible with various enzymes and does not

cause protein modifications
10
15
20
25
30
10
15
min
Does not inhibit enzyme activity, unlike conventional denaturants
Helps reduce digestion time and amount of required protease
Improves the digestion of enzyme resistant proteins such as

membrane proteins
Acid labile surfactant, the degradation products from RapiGest do

not interfere with LC/MS or MALDI MS analyses
20
25
30
min
How RapiGest SF works
RapiGest SF
Cleavage Sites
Cleavage Sites
Use of RapiGest SF to Assist in Protein Deglycosylation

A) Control
Deconvoluted Mass
RapiGest shows significant advantage over other denaturants

since it is not disruptive to endoprotease activity.
B) 0.1% OG
Trypsin *
Solution
C) 0.1% RapiGest SF
800
1000
1200
1400
m/z
1600
1800
2000
42000
43000
44000
mass
45000
46000
LC/MS spectra of deglycosylated ovalbumin are shown. A) Ovalbumin was solubilzed without the use of denaturant, and was not deglycosylated. B) Ovalbumin was denatured using 0.1% n-octyl-b-glycopyranoside (OG) and
deglycosylated. C) Ovalbumin was denatrued in 0.1% RapiGest SF solution and deglycosylated. The MS scans
were deconvoluted to the MW of the protein. Complete deglycosylated was observed after 2 hours deglycosylation
for the RapiGest SF solubilized ovalbumin.
Trypsin
Activity (%) **
No additive
0.1% RapiGest
0.5% RapiGest
0.1% SDS
0.5% SDS
100
100
100
24
1
(CH2)3SO3-Na+
67
29
87
97
83
CH3(CH2)10
Low pH
CH3(CH2)10
Literature
References
www.waters.com
0.1% SDS/0.1% RapiGest

50% Methanol
50% Acetonitrile
1M Urea
2M Urea
O
O
A complete peptide mapping

of membrane proteins: a
novel surfactant aiding the
enzymatic digestion of bacteriorhodopsin, Yu YQ, Gilar M,
Gebler JC, Rapid Commun
Mass Spectrom. 2004; 18
(6):711-5.
Trypsin
Activity (%) **
* .5 g of trypsin in 50 mM ammonium bicarbonate, pH 7.9; 0.2 mM of BEAA

** Measured as delta BEAA absorbance @ 253 nm (slope within 5 min)
Enzyme-Friendly, Mass
Spectrometry-Compatible
Surfactant for In-Solution
Enzymatic Digestion of
Proteins, Yu YQ, Gilar M, Lee
PJ, Bouvier ES, Gebler JC,
Anal Chem. 2003 Nov 1;
75(21):6023-8.
Trypsin *
Solution
O
HO
(CH2)3SO3-Na+
OH
RapiGest hydrolyzes under acidic condition (half life time = 7.6 minutes at pH 2).
Therefore, it is compatible with LC/MS and MALDI MS analysis.
A rapid sample preparation method for mass

spectrometric characterization of N-linked glycans,
Ying Qing Yu, Martin Gilar,
Jennifer Kaska and John C.
Gebler, Rapid Commun Mass
Spectrom. 2005;
19: 2331-2336.
Description
Rapi Gest SF 1 mg vial
Rapi Gest SF 1 mg vial (5 pack)
Rapi Gest SF Custom
Part No.
186001860
186001861
186002123
186002122
186002118
257

MassPREP On-Line Desalting Devices
Effectively desalts proteins yielding improved LC/MS results
Fast on-line method for high throughput applications
Excellent protein recoveries and no detectable carryover
>100 injections from a single cartridge
Excellent Recovery with No Detectable Carryover

Competitors On-line Desalting Cartridge
Inject #4 = 10 L PBS Blank
Inject #3 = 10 L mAb
(1 g/L PBS)
Inject #2 = 10 L PBS Blank,
following a 5 g mAb injection
T he MassPREP on-line desalting column can effectively desalt proteins

prior to LC/MS analyses. Because non-volatile salts (e.g., NaCl) can
suppress ionization of intact proteins leading to poor detection sensitivity, it is important to remove or significantly minimize the introduction
of these compounds into the mass analyzer. T he reversed-phase, phenyl
material contained in MassP REP on-line column successfully traps
proteins, allowing the salts to be washed to waste prior to protein elution
into the mass spectrometer. With an optimized LC/MS method, cycle
times as low as 4 minutes (for intact antibody) and 10 minutes (for
reduced antibody) are achievable.
Description
Qty.
Part No.
MassPREP Micro Desalting Column

MassPREP On-line Desalting Cartridge (2.1 x 10 mm)*
UPLC Intact Mass Analysis Application Kit** (Includes MassPREP
Micro Desalting Column and ACQUITY Tubing Kit)
Sentry 2.1 x 10 mm Guard Cartridge Holder.*
Required for use of MassPREP On-line DesaltingCartridge
1/pk
2/pk
186004032
186002785
1/pk
176001519
1/pk
WAT097958
** See: UPLC Intact Mass Analysis Application Kit Manual (715001664)
Waters MassPREP On-Line Desalting Cartridge (2.1 x 10 mm)

Experimental
LC System: Alliance 2796 Separation Module
MS System: Q-Tof micro, ESI Positive
Eluent A: 0.1%Formic Acid (H2O) Eluent B: 0.1% Formic Acid (ACN)
Waters MassPREP On-line Desalting Cartridge
(1 g/L PBS)
Column-related carryover from previous protein sample injections can

compromise the integrity of collected LC/MS data. Compared to results obtained
on a competitive on-line desalting cartridge (top), excellent sample recovery is
obtained with Waters MassPREP on-line desalting cartridge (bottom).
Waters MassPREP Micro Desalting Column (2.1 x 5 mm)

Experimental
LC System:
MS System:
Ionization Mode:
Eluent A:
Eluent B:
Column temp:
Waters ACQUITY UPLC system

Waters LCT Premier ESI-TOF MS/Synapt HDMS
ESI Positive, V mode
0.1% Formic Acid (H2O)
0.1% Formic Acid (ACN)
80 C
Pre-run Blank
Max:2 Counts
0.5 g Intact IgG1
754 Counts
Post-run Blank
5 Counts

(1 g/5 L PBS)
Inject #98 = 5 L BSA

(1 g/5 L PBS)
Combined ESI-TOF mass spectra of an intact IgG1 antibody from a 4 minutes LC/MS analysis.
The results reveal no detectable carryover following a 0.5 g injection of the antibody.
MS
Light Chain
MS
Heavy Chain
TIC
(1.3 g load)
Over a series of 100 injections, satisfactory results were obtained for BSA and a mAb,
as shown for injections #97-100 on a MassPREP on-line desalting cartridge.
Reference Desalting of Proteins Using MassPREP On-line Desalting Cartridges Prior to
Mass Spectrometry. 2005 Waters Applications Note 720001077EN
258
Traps, Columns, and Cartridges
Total ion chromatogram (TIC) from UPLC/MS analysis of light and heavy chains
from a reduced IgG1 antibody. A 10 min LC/MS run largely resolved the earlier
eluting light chain from the later eluting glycosylated heavy chains.
www.waters.com

NanoEase Trap, Capillary, and Nano Columns
Waters NanoEase Trapping, Capillary, and Nano columns, contain carefullyselected, reversed-phase and ion-exchange chemistries for bioseparations.
T he column offerings are developed and intended for use on various traditional low pressure, capillary, and nano flow LC Systems.
NanoEase Trapping Columns

BSA Digest Using NanoEase Symmetry300 C18 Trap Columns
NanoEase trap direct connect design minimizes

band broadening
SCX offering for 2D applications
Column:
RP offerings for sample desalting or concentration
Trapping Columns:
Mobile Phase:
NanoEase Symmetry300 C18

180 m x 150 mm
NanoEase Symmetry300 C18 Trap
A: 98% H2O/2% ACN/0.1% FA
B: 98% ACN/2% H2O/0.1% FA
Time
%A
%B
0.00
95.0
5.0
30.00 50.0
50.0
31.00 20.0
80.0
41.00 20.0
80.0
3.6 L/min
1 L
20 C
Gradient Conditions:
Flow Rate:
Injection Volume:
Temperature:
MS Conditions:
Ion Source:
Source Temperature:
Desolvation
Temperature:
Instrument:
Symmetry300 C18, Atlantis dC18, and strong cation-exc hange (SCX)

NanoEase trap columns are robust devices that can effectively separate
complex samples or remove buffers and high concentrations of salts through
many injections. T he reversed-phase trap design minimizes peak band
broadening while the design of the SCX device provides increased binding
capacity. T hese devices generate low backpressure and yield good
recovery to the subfemtomole peptide level. In addition, NanoEase
trap column direct connect design allows for easy configuration and
replacement in a six or a ten-port valve for either one-dimensional or
two-dimensional LC/MS analyses.
10
12.5
15
17.5
20
Description
Symmetry300 C18 Trap Column 5/pack
Atlantis dC18 Trap Column 5/pack
SCX 300 Trap Column 5/pack
Symmetry C18 Trap Column 5/pack
0.18 i.d. Custom Trap Column 5/pack
0.50 i.d. Custom Trap Column 5/pack
22.5
25
Electrospray positive
80 C
150 C
Waters CapLC with
Q-tof micro technology
27.5
30 Min.
Particle Size
Dimension
Part No.
5 m
5 m
5 m
5 m
Custom
Custom
0.18 x 23.5 mm
0.18 x 23.5 mm
0.50 x 23.5 mm
0.18 x 23.5 mm
0.18 x 23.5 mm
0.50 x 23.5 mm
186002622
186002574
186002623
186002808
186002687
186002806
NanoEase Trap Column hardware obtained from Optimize Technologies Inc., Oregon City, OR, 97045-0009, USA
www.waters.com
259

NanoEase Nano and Capillary Columns
NanoEase columns contain high performance, small particle sorbents.
Exacting manufacturing and QC production procedures ensure consistent
column performance. Superior column longevity is ensured by a novel
frit tec hnology that minimizes bed disturbance throughout the life of
the column.
Superior chromatographic performance
Available in variety of Waters stationary phases
Robust and easy to handle
Can be used with any capillary LC system
NanoEase LC/MS Nano Column Reproducibility

500 fmol Enolase Digest
Column:
Gradient:
Flow Rate:
System:
NanoEase Symmetry C18,

3.5 m, 75 m x 100 mm
0.1% FA in water
0.1% FA, 2% water in acetonitrile
Eluent A:
Eluent B:
5-50% B in 60 min
400 nL/min
Waters CapLC operated in
split flow mode, Q-Tof micro
100
TOF MS ES+
TIC
9.78e4
Inj #4
100
%
Inj #3
TOF MS ES+
TIC
1.48e5
Inj #2
TOF MS ES+
TIC
1.71e5
Inj #1
TOF MS ES+
TIC
1.69e5
100
%
0
100
%
0
10
Description
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry300 C18
Atlantis dC18
Atlantis dC18
Symmetry C18
Symmetry C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Inner Diameter
Length
Particle Size
Part No.
75 m
75 m
75 m
75 m
75 m
75 m
75 m
75 m
75 m
100 m
100 m
100 m
100 m
100 m
100 m
150 m
150 m
150 m
150 m
150 m
150 m
150 m
150 m
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
150 mm
100 mm
150 mm
50 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3 m
3 m
3 m
3.5 m
3.5 m
3.5 m
3.5 m
3 m
3 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3 m
3 m
3 m
186002188
186002189
186002190
186002191
186002192
186002193
186002194
186002195
186002197
186002201
186002202
186002203
186002206
186002208
186002209
186002459
186002461
186002462
186002463
186002464
186002466
186002467
186002468
Description
Custom
Custom
Custom
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Symmetry300 C18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Atlantis dC18
XBridge BEH130 C18
Custom
20
30
40
50
60 Min.
Inner Diameter
Length
Particle Size
Part No.
75 m
100 m
150 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300 m
300m
Custom
Custom
Custom
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
100 mm
150 mm
50 mm
Custom
Custom
Custom
Custom
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
3.5 m
3.5 m
3.5 m
5 m
5 m
5 m
3 m
3 m
3 m
5 m
5 m
5 m
5 m
Custom
186002213
186002214
186002472
186002581
186002582
186002583
186002584
186002585
186002586
186002587
186002588
186002589
186002590
186002591
186002592
186002593
186002594
186002595
186002596
186002597
186002598
186003682
186002605
NOTE: Use of Waters 300 m Capillary Inlet Tubing (Part Number 430001471) and 300 m Capillary Outlet Tubing (Part Number
430001472) required for use with 300 m I.D. capillary columns. Waters Kit Part Number 700002757 contains both the 300 m
Capillary Inlet Tubing (Part Number 430001471) and the 300 m Capillary Outlet Tubing (Part Number 430001472).
For Peptide Separation Technology NanoEase and Capillary Columns, See Page 207
260
www.waters.com

nanoACQUITY UltraPerformance LC Columns
Novel frit technology and design for extended column life
Capable of operation up to 10,000 PSI
Easily interfaces with LC/MS/MS systems
Available with ACQUITY UPLC C18, 1.7 m BEH Technology particles
Separation of Enolose Digest

200 fmol enolase digest, 5% B - 60% B in 30 minutes, 250 nL/min flow rate
E041110mds04
23.42 24.98
TOF MS ES+
TIC
7.93e3
20.02 20.48
22.44
nanoACQUITY UPLC System
28.21
Peak widths: 3.6 to 4.8 sec

column backpressure ~ 3,300
17.59
16.41
19.18
27.62
32.36
14.73 15.63
25.54
15
20
29.46 29.85
25
30
35 Min.
The ability to operate at higher pressures enables the use of longer columns
(up to and including 250 mm in length) and further leverage the separation
power of 1.7 m particles. Thus, the nanoACQUITY UPLC chemistry
offering now includes the Peptide Separation Technology nanoACQUITY
UPLC columns, which incorporate the 1.7 m BEH130 material.
Increased Peak Capacity Separations

Waters nanoACQUITY Trapping, Capillary, and Nano columns are designed
specifically for use on Waters nanoACQUIT Y UPLC System. As with our
line of NanoEase columns, these offerings containing carefully selected
reversed-phase and ion-exchange chemistries for bioseparations.
3 m conventional column
Pressure 3,500 PSI
Peak Capacity 297
100 fmoles of a mixture of 5 MassPREP Digest Standards*

75 m i.d x 300 mm (custom length)
3-50% B 120 min
250 nL/min
1.7 m BEH
Pressure 9,500
Peak Capacity 458
Column i.d.
Flow Rate
300 m
4 L/min
150 m
1 L/min
75 m
250 nL/min
30
40
50
60
70
80
90 Min.
30
40
50
60
70
80
90 Min.
* Mixture includes:
MassPREP Phosphorylase b Digestion Standard
MassPREP Bovine Hemoglobin Digestion Standard
MassPREP ADH Digestion Standard
MassPREP BSH Digestion Standard
Part Number
186002325
186002326
186002327
186002328
186002329
Flow Rates and Column Internal Diameters for Capillary and Nanoflow. Capillary-scale separations
are done in a flow rate range of 1 l/min to 10 l/min on columns with internal diameters (i.d.)
ranging from 300 m to 100 m. Nanoflow is <1 l/min on columns with i.d. 75 m.
www.waters.com
261
MS and LC/MS ConSuMabLeS for bioMoLeCuLeS

nanoACQUITY UPLC Columns (10,000 psi)
Descript ion
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Symmetry C18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Atlantis dC18
Atlantis dC18
nanoACQUITY UPLC Trap
SCX (std fitting)
Particle
Size
Inner
Diameter
Length
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3.5 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
3 m
75 m
75 m
100 m
100 m
150 m
150 m
300 m
300 m
75 m
75 m
100 m
100 m
150 m
150 m
300 m
300 m
100 mm
150 mm
100 mm
150 mm
100 mm
150 mm
100 mm
150 mm
100 mm
150 mm
100 mm
150 mm
100 mm
150 mm
100 mm
150 mm
186003491
186003492
186003493
186003494
186003495
186003496
186003497
186003498
186003499
186003500
186003501
186003502
186003503
186003504
186003505
186003506
180 m
23 mm
186003507
Part No.
Descript ion
nanoACQUITY UPLC
Trap, Symmetry C18,
(std fitting)
nanoACQUITY UPLC
Trap Symmetry C18,
(v/v fitting)
Custom
Particle
Size
Inner
Diameter
Length
Part No.
5m
180 m
20 mm
186003514
5m
180m
20mm
186004630
186003513
Peptide Separation Technology Columns

BEH130 C18
BEH130 C18
BEH130 C18
BEH130 C18
BEH130 C18
BEH130 C18
BEH300 C4
BEH300 C4
BEH300 C4
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
1.7 m
75 m
75 m
75 m
75 m
100 m
150 m
75 m
100 m
150 m
100 mm
150 mm
200 mm
250 mm
100 mm
100 mm
100 mm
100 mm
100 mm
186003542
186003543
186003544
186003545
186003546
186003550
186004639
186004640
186004641
For use with nanoACQUITY UPLC systems rated to 10,000 psi only.Not for use with nanoACQUITY UPLC systems rated to 5,000 psi.
LC/MS Kits for the nanoACQUITY UPLC System with 2D Technology

Two-dimensional (2D) LC/MS Kits for the nanoACQUIT YUPLC System
with 2D technology provide high efficiency on-line 2D-LC/MS analysis for
complex proteomics samples, such as protein global digests. Two kit options
are available: nanoACQUITY UPLC On-line SCX/RP 2D Separations kit and
nanoACQUITY UPLC On-line RP / RP 2D Separations kit.
nanoACQUITY UPLC On-line SCX/ RP 2D Separations kit

T he first-dimension separation uses a strong cation-exchange nanoACQUITY
U P LC 180 m x 23 mm strong cation exc hange (SCX ) column in
combination with a nano-scale, reversed-phase trapping column (Symmetry
C18, 5um) followed by and a nanoACQUIT Y UPLC Bridged-Ethyl Hybrid
(BEH), C18, 1.7 um BEH analytical column to perform highly resolving
orthogonal separations.
Description
Part No.
nanoACQUITY UPLC on-line SCX/RP 2D-LC/MS kit

Kit consists of:
Assembly, 25 m, capillary, BSM (binary solvent manager)
to trap valve
Assembly, 40 m capillary, ASM (auxiliary solvent manager)
to injection valve
Assembly, capillary tubing, injection valve to trap valve
Assembly, capillary tubing, injection to trap valve
nanoACQUITY UPLC SCX column, 5 m, 180 m x 23 mm
nanoACQUITY UPLC trapping column, 5 m Symmetry C18,
180 m x 20 mm
nanoACQUITY UPLC analytical column, 1.7 m BEH C18,
75 m x 100 mm
MassPREP 5 Protein Digest Standard Kit
nanoACQUITY UPLC on-line 2D-LS/MS Users Guide
262
176001367
nanoACQUITY UPLC On-line RP/ RP 2D Separations kit

Waters supports an on-line separation protocol based upon a reversed-phase
separation at pH 10 followed by reversed-phase separation at pH 2 (RP/
RP) performed on our pH tolerant XBridge BEH, C18 particle and ligand
bonding technology (ref 1). T his orthogonal separation technology uses
highly resolving, sub-3 micron reversed-phase particles to exploit the ionic
and hydrophobic characteristics of peptides to increase peak capacity and
provide better protein identifications.
Description
430001575
Part No.
Replacement Items for nanoACQUITY UPLC On-line RP/RP 2D Separations kit

Assembly, Capillary Tubing with Frit 40 m x 16
1
430001571
1
430002140
1
430002153
1
430002155
2
430002183
nanoEase BEH130, C18, 5 um, 300 m x 50 mm
1
186003682
nanoACQUITY UPLC Trap, Symmetry C18,
(standard fitting), 5 m, 180 m x 20 mm
1
186003514
nanoACQUITY BEH130 C18, 1.7 m, 75 m x 100 mm
1
186003542
430001576
430001577
430001629
186003507
186003514
186003542
186002330
715001357
Qty.
Stapels, M and Fadgen, K (2009) A Reproducible Online 2D Reversed Phase High-Low pH Method
for Qualitative and Quantitative Proteomics Current Trends in Mass Spectrometry
www.waters.com

MassPREP Phosphopeptide Enrichment Kit
Phosphopeptide Analysis without (top) and with (bottom)
MassPREP Phosphopeptide Kit Enrichment
Column:
Trapping Mode:
Column:
Solvent A:
Solvent B:
Flow Rate:
Gradient:
Injection Volume:
Symmetry C18 nanoACQUITY Trapping Column, 5 m, 180 m x 20 mm.

5 l/min for 3 minutes (100% aqueous).
nanoACQUITY Atlantis dC18, 3 m, 75 m x 100 m
0.1% formic acid in 100% Milli Q water
0.1% formic acid in 100% acetonitrile
300 nl/min
2% -40% B, 1% B per minute
2 l
A) Control
T18_1P
T19_1P
Enolase peptides :
4 phosphopeptide stdandard
in 1:1 molar ratio
Selective enrichment of phosphopeptides from complex

proteomic samples
Highly efficient and reproducible affinity-based enrichment
Easy to use
Application in high-throughput analysis
T43_1P
T43_2P
B) After Enrichment
No Non-Specific
Binding
MassPREP Phosphopeptide Enrichment Kit, developed for selective enrichment of phosphopeptides from complex samples, includes a 96-well microscale, solid-phase extraction (SPE) plate packed with an affinity sorbent.
T he kit also includes a unique chemical (Enhancer) that can be added to
further improve the selectivity of phosphopeptides. In contrast to currently
popular immobilized metal affinity chromatography (IMAC) technology,
this isolation method does not require pre-loading the sorbent with metal
chelator. T his phosphopeptide enrichment protocol is simplified. T he robustness of this method is provided by the sorbent that has high native affinity
towards phosphopeptides and the Enhancer which yields superior selectivity
of the sorbent towards phosphopeptides.
20
30
Description
40
50 Min.
Units/Kit Amt/Unit
MassPREP Phosphopeptide Enrichment Kit

Kit Consists of:
MassPREP Phosphopeptide Enrichment Elution Plate 1
MassPREP Enolase/Phosphopeptide Standard
1
MassPREP Enhancer
10
MassPREP Enhancer
5
Part No.
186003864
1 plate
1 nmole
500 mg
500 mg
186003820
186003286
186003821
186003863
For more information see Phosphopeptide Standards on page 255
Waters Protein Expression System Kits

T he Protein Expression System consists of nanoACQUITY UPLC system,
Q-Tof Premier, MassLynx ProteinLynx Global Server bioinformatics
software, and Waters MassPREP chemistry consumables and kits.
MassPREP Chemistry Consumable Kits for the Protein Express System

include nanoACQUIT Y UPLC columns, MassP REP Protein Digestion
Standards Mix 1 and 2, MassPREP Enolase Digest Standard, and RapiGest
SF Surfactant. Both Microscale and a Nanoscale Kits are available.
Microscale Kit
Nanoscale Kit
Description
Microscale Kit
Kit Contains :
nanoACQUITY UPLC 300 m x 150 mm
Atlantis dC18 3 m Column
MPDS Mix 1
MPDS Mix 2
MPDS Enolase
RapiGest SF, 1 mg
www.waters.com
Qty.
Part No.
186003903
1
1
1
1
1
186003506
186002865
186002866
186002325
186001860
Description
Qty.
Nanoscale Kit
Kit Contains :
Atlantis dC18 3 m Column
Symmetry C18 5 m Trap Column
MPDS Mix 1
MPDS Mix 2
MPDS Enolase
RapiGest SF, 1 mg
Part No.
186003904
Kits
186003500
1
1
1
1
1
186003514
186002865
186002866
186002325
186001860
263
UPLC TEChNOLOGY FOR bIOMOLECULES

UPLC Technology for Biomolecules
Waters offers a collection of powerful tools for c haracterizing biopharmaceuticals. T hese solutions are designed specifically to provide more
accurate, reliable, and robust results, simplify your analyses and thereby
increase productivity. Our system solutions combine Waters market-leading
instrumentation, column chemistries, and software and informatics products,
that leverage the sensitivity and resolution of Ultra Performance LC
(UPLC) Tec hnology.
UPLC Technology was introduced by Waters as a novel chromatographic
technique that takes full advantage of sub-2 m column chemistries used
in a system optimized for pressures necessary to produce best-in-class
separations. By packing columns with very small particles, band-broadening
during separation is minimized. In addition, since distances are reduced in
small particles, separations can be optimized using higher linear velocities
(i.e., flow rates), while maintaining chromatographic equilibrium. T he result
of these chromatographic developments is separations with dramatically
increased resolution, sensitivity, and speed.
T he properties of these columns places a new level of demand on the
c hromatographic system, and Waters has holistically developed a family
of U PLC instruments, including t he binary-based ACQUIT Y U PLC,
nanoACQUITY UPLC, and PAT ROL UPLC systems and the quaternary-based
ACQUITY UPLC H-Class and ACQUITY UPLC H-Class Bio systems, to take
advantage of these chromatographic principles.
264
To gain the benefits of the small particles columns, the system must
generate exceptionally accurate and precise gradients at relatively high
operation pressures. T he sample manager must provide accurate, smallvolume injections with minimum cycle time. Furthermore, the detectors
must truly minimize band-broadening while providing high signal-tonoise ratios for peaks that are narrow in both time and volume.
Based on chromatographic principles, Waters synergistically developed the
columns and the instrumentation that combine to make UPLC technology a
realityand now a proven success in laboratories around the world. W hile
the first successful application of UPLC technology was in small molecule
pharmaceuticals, Waters dedication to developing impactful laboratory
innovations quickly saw the benefits of UPLC tec hnology applied to the
separation of biological and bioc hemically-significant macromolecules.
U PLC tec hnology brings a new level of separation and detection
performance to the analysis of biomolecules, whether from complex
peptide mixtures or from a potentially life saving monoclonal antibody
biotherapeutic. Today, Waters UPLC technology provides improvements
in the resolution, sensitivity, separation, and detection efficiency for
peptides,proteins, amino acids, synthetic DNA and RNA oligonucleotides,
and glycans t hat help scientists and c hromatographers ac hieve
desired objectives.
www.waters.com
UPLC TeChnoLogy for BiomoLeCULes

Peptide
Mapping
Amino Acid
Analysis
Waters UPLC Peptide Analysis Solution, including UPLC Peptide Separation

Technology columns, delivers maximum resolution and sensitivity. UPLC
chromatographic improvements have lead to greater confidence in protein
identification with improved determination of modified peptides, including
glycopeptides. In the comparison of separations using 3.5 m particles
and 1.7 m particles, peaks are clearly sharper, and more small peaks are
resolved using UPLC technology.
T he UPLC Amino Acid Analysis Solution is a total application solution

optimized for an accurate, reliable, and reproducible analysis of amino
acids. Combining Waters new AccQTag Ultra c hemistry and ACQUIT Y
UPLC system, performance is assured in the areas of protein characterization, cell culture monitoring, and the nutritional analysis of foods and
feeds. T he enhanced resolution and sensitivity of the separation ensures
that the analysis yields accurate and precise qualitative and quantitative
results. T his complete solution includes the instrument, derivitization and
separation chemistry, software, and comprehensive support for a true amino
acid analyzer solution.
Protein
Analysis
UPLC/MS tec hnology is available for rapid, efficient mass profiling of

protein samples, including intact antibodies and their associated variants.
Samples can be successfully desalted on-line using the UPLC Intact Mass
Analysis Kit, which ensures a high resolution spectrum with good signalto-noise. T he resulting total ion chromatograms (TICs), combined ESI-TOF
mass spectra, and MaxEnt1 deconvolution mass spectrum of the intact
IgG1 quickly confirm the identity of the molecule while providing a
measure of the degree of modification of the protein in this sample.
Reversed-phase separations of proteins are improved wit h U PLC
Protein Separation Tec hnology columns, used with ACQUIT Y UPLC.
T he BEH300 C 4 column gives good peak shape for wide range of
proteins with minimized carryover. T his column chemistry is available in
3.5 and 1.7 m particles for UPLC separations and can be effectively used
to analyze reduced and alkylated monoclonal antibodies.
Our new ACQUIT Y UPLC BEH200 SEC, part of our ACQUIT Y UPLC SEC
Solution, were specifically designed for the characterization of proteins
and their aggregates. In addition, our Protein-Pak Hi Res IEX column family
are well suited for use on ACQUITY UPLC Technology for the analysis of
protein charge variants such as lysine clips on monoclonal antibodies.
www.waters.com
Synthetic
Oligonucleotides
Analysis
T he UPLC Oligonucleotide Analysis Solution and UPLC Oligonculeotide

Separation Tec hnology (OST ) columns provide higher resolution in less
time of synthetic oligonucleotide target products from contaminating
failure sequences. T his Waters tec hnology leads to better resolution
and increased sample throughput compared to use of traditional
HPLC methods.
Glycan
Analysis
T he Waters UPLC Glycan Analysis Solution meets the needs of these

applications. T he ACQUIT Y UPLC BEH Glycan Separation Tec hnology
column is optimized for use wit h t he ACQUIT Y U PLC System wit h
fluorescence detection (FLR), separating the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives. W hen combined
for use in a biopharmaceutical or pharmaceutical laboratory to monitor
glycan profiles, the UPLC Glycan Separation Solution provides robust, highresolving, reproducible, and rapid methods that out-perform analyses
by HPLC.
265
INT RODUCING ACQUITY UPLC CSH AND XSELECT COLUMNS

POW ERED BY CSH TECHNOLOGY.
CSH Tec hnology empowers your UPLC and HPLC
systems to perform better than ever before. Sharp
peak shapes. High efficiency. High loading capacity.
Rapid equilibration after mobile-phase changes.
pH stability and improved batc h-to-batc h
reproducibility for ionized compounds. Improve
the performance of every LC instrument in
your lab get ACQUIT Y UPLC CSH and
XSelect HPLC columns today. Learn more
at www.waters.com/csh
2010 Waters Corporation. Waters, CSH, ACQUITY UPLC,

XSelect, UPLC and The Science of What
s Possible
are trademarks of Waters Corporation.
[ Size Exclusion Chromatography

GPC & GFC ]
Introduction ...........................................................................................................................................................................................268
Selecting a Column ..................................................................................................................................................................................268
Column Bank Optimization .....................................................................................................................................................................269
GPC Column Selection Guide ...................................................................................................................................................................270
GPC Solvent Selection Guide ...................................................................................................................................................................271
GPC Product and Ordering Information
Styragel HR High-Resolution Columns ...................................................................................................................................272
Styragel HT High-Temperature, High-Stability Columns .......................................................................................................273
Styragel HMW High Molecular Weight Columns ....................................................................................................................274
HSPgel Columns .......................................................................................................................................................................275
Shodex GPC Columns ................................................................................................................................................................276
Envirogel High-Resolution GPC Cleanup Columns .................................................................................................................277
Preparative GPC Columns .........................................................................................................................................................277
Aqueous SEC Columns
Selection Guide.........................................................................................................................................................................278
Ultrahydrogel Columns ............................................................................................................................................................279
Calibration Standards
Organic ......................................................................................................................................................................................280
Aqueous ....................................................................................................................................................................................280
267
SIzE ExCLUSION ChROMaTOGRaPhYGPC & GFC
Introduction
T he Gel Permeation Chromatography (GPC) technique, pioneered by J.C.

Moore of the Dow Chemical Company1 in the early 1960s, is a mode of
HPLC for separating molecules based upon their effective size in solution.
Gel Filtration of Water Soluble

Polymers and Proteins
Originally patented and licensed to Waters as a method for characterizing

polymers, GPC is also useful for separating small molecules from interfering matrices such as those found in foods, pharmaceutical preparations,
and natural products. In addition, GPC is often used as the first step in the
sequential analysis of complex, unknown, organic mixtures. In these analyses, the unknown mixture is separated by size in the GPC mode, purified
fractions are collected, and the fractions are subsequently separated based
on chemical differences by normal, or reversed-phase chromatography.
Water soluble polymers and proteins can be routinely separated by size in

solution. Size separations in aqueous solutions, often called gel filtration,
are commonly used as the first step in the analysis of a complex protein
mixture and as an important tool to characterize the molecular weights of
water soluble polymers such as polyacrylamide.
Small Molecules in Organic

or Aqueous Solution
Small molecules (less than 2,000 MW) can generally be separated if their
size in solution differs by approximately 10%. GPC can also easily detect
the presence of high molecular weight components in a sample matrix
indicating the need for sample cleanup prior to analysis of small molecules
by normal, or reversed-phase chromatography.
GPC of Organic Soluble Polymers

GPC is a practical, easy, and convenient tool for determining the complete
molecular weight distribution of a polymer. Any oligomers, monomers and
additives in a complex polymeric solution can also be separated as long as
there exist significant size differences among these components.
GPC is an officially recognized method in the plastics industry and has
been adopted by the American Society for Testing and Materials (ASTM,
Committee D-20) for determining the molecular weight distribution of
polymers.2
268
Size Exclusion ChromatographyGPC & GFC
Selecting a Column
For over 40 years, Waters has been the market leader in GPC analysis,
providing the highest quality GPC products and expert applications support.
To solve unique separation problems across a broad spectrum of applications, we manufacture a wide range of GPC columns and accessories. Our
series of Ultrastyragel and Styragel columns have been the standard
in GPC columns for the separation of organic-soluble samples. To solve
your specific problems in aqueous separations, you can select from our
Ultra hydrogel columns.
Waters offers calibration standards for organic and aqueous GPC/SEC.
Standards are available as individual molecular weights, or come in kits
containing a range of MW standards.
Waters Styragel Columns for

Polymer Characterization
Designed specifically for polymer c haracterization, the Styragel
columns are grouped into the HR series for low-to-mid molecular
weight samples, the HT series for high-temperature applications, and
the HMW series for ultra-high molecular weight samples. Specially
controlled styrene divinylbenzene formulations provide reproducible
performance in your GPC applications.
1
J.C. Moore, J. Polymer Science, A2, 835 (1964)
ASTM D3536-76, D3593-80 and D3016-78
www.waters.com

Waters Styragel HR Columns
Column Bank Optimization
T he Styragel HR ( high resolution) series of columns were specifically

developed for the analysis of low-to-mid molecular weight samples. T he
columns are packed with rigid 5 m styrene divinylbenzene particles and
deliver the maximum resolution and efficiency required for low
molecular weight analysis.
Using the proper column is essential to optimum performance. T he rule

for selecting the best column(s) for an analysis is straightforwardprovide
separation only for the molecules which you wish to separate. Never specify
a column with a higher exclusion limit than the exclusion limit required by
the largest molecules you wish to separate. W hen the measurement of
broad distributions is desired, mixed bed or extended range columns are
appropriate, thereby resulting in separation power that is constant at all
molecular weight sizes.
Waters Styragel HT Columns

T he Styragel HT ( high temperature) series of columns were specifically
developed for use in the mid-to-high molecular weight range. T he columns
are packed with rigid 10 m styrene divinylbenzene particles and can be
used at ambient or high temperature while still maintaining excellent
resolution. T he narrow particle size distribution results in a more stable
packed bed structure making Styragel HT columns extremely durable.
Waters Styragel HMW Columns

T he Styragel HM W ( high molecular weight) series of columns were
specifically developed for the analysis of shear sensitive, ultra-high
molecular weight polymers. Styragel HMW columns are packed with
rigid 20 micron styrene divinylbenzene particles and fitted with specially
designed high porosity frits which minimize polymer shear effects. T he
columns may be used at either ambient or elevated temperature and
exhibit excellent column lifetime.
Waters Styragel column offering is comprised of mixed-bed and narrow

molecular weight range columns. T he mixed -bed columns, designated
E for extended range, are ideal for use as scouting columns when the
molecular weight range of your sample is unknown or for the measurement
of samples with broad distributions. T he narrow molecular weight range
columns deliver greater pore volume and resolution in a more concentrated
molecular weight range and are a muc h more powerful tool for obtaining more precise molecular weight information.
Your choice of conventional 7.8 mm i.d. or solvent-efficient 4.6 mm

i.d. columns In addition to the conventional 7.8 mm columns, the three
Styragel series include 4.6 mm i.d. columns in both the single-pore and
mixed-bed columns. T hese Styragel narrow-bore columns can cut your
solvent consumption and disposal costs by as much as two thirds. W hen
used with low-dispersion GPC systems, our 4.6 mm columns match the
high-performance of our 7.8 mm columns.
We also carry a full range of high resolution aqueous GPC columns,
preparative GPC columns and calibration standards.
Styragel Guard Column
Individual pore-size columns deliver up to 2.5X the resolution of mixed-bed columns.
T he Styragel 4.6 mm i.d. x 30 mm guard column is designed to increase

the lifetime of your Styragel analytical column. T he guard column can be
used in series with any Waters conventional organic GPC column.
www.waters.com
269
GPC Column Selection Guide

Effective Molecular Weight Range
101
102
103
104
105
106
107
108
Ultra-High Molecular Weight Analysis

Waters Styragel HMW Series
20 m particle size
Ambient to 150 oC
Styragel HMW 7
Applications:
UHDPE
UH-Polystyrene
UH-Isoprene
UH-PMMA
Styragel HMW 6E
Styragel HMW 2
Mid-Range Molecular Weight

Waters Styragel HT Series
10 m particle size
Ambient to 150 oC
Styragel HT 6E
Styragel HT 6
Applications:
ABS
Acetyl
Acrylics
EVA
Nylon
PEEK
PET/PBT
Styragel HT 5
Styragel HT 4
Styragel HT 3
Styragel HT 2
Styragel HR 5E
Low Molecular Weight Analysis

Waters Styragel HR Series
5 m particle size
Ambient to 80 oC
Styragel HR 4E
Styragel HR 6
Applications:
Additives
Epoxy
Oligomers
Phenalics
Unsaturated Polyester
Urea/Formaldehyde
Polyethylene Glycol
Ethanolamines
Melamine Resin
Styragel HR 5
Styragel HR 4
Styragel HR 3
Styragel HR 2
Styragel HR 1
Styragel HR 0.5
Effective Molecular Weight Range
270
101
102
103
104
105
106
107
108
www.waters.com
GPC Solvent Selection Guide
www.waters.com
Polymer
GPC Solvent
Polyisobutylene
Toluene
Polybutylene
Chlorinated Rubber
Polybutadiene
Polyisoprene
Polydimethylsiloxane
Toluene/75%
Chlorinated Polyethylene
Polyethylene Ethylacrylate
Polyethylene Vinylacetone
Polyethylene Methacrylic acid
Polyphenyleneoxide
Poly-4-methylpentene(1)
Polyethylene
TCB/135 - 160 C
Ultra-high Molecular Weight

Polyethylene
Polypropylene
TCB/135 - 160 C
Polyetheretherketone
Polyetherketone
Phenol/TCB 1:1/145 C
Polycarbonate
Methyl Chloride
Polyglycolic acid
gamma-Butyl Lactone
Acrylonitrile Methylmethacrylate
Cellulose Acetate
Cellulose Acetate Butyrate
Cellulose Acetate Proprionate
Cellulose Nitrate
Cellulose Proprionate
Cellulose Triacetate
Diallyl Phthalate
Ethyl Cellulose
Epoxy
Polyester Alkyd
Polybutene(1)
Polybutadiene Styrene
Phenol Formaldehyde
Phenol Furfural
Polymethylmethacrylate
Polypropyleneglycol
Polystyrene
Polysulfone
Polyvinylacetate
Polyvinylbutyral
Polyvinylchloride
Polyvinylchloride Acetate
Polyvinyldienechloride
Polyvinylformal
Polystyrene Acrylonitrile
Polystyrene Alphamethylstyrene
Polyester T hermoset
Phenolics
Rosin Acids
Polyglycolic Acid
THF/40 C
Melamine Formaldehyde
Nylon (All types)
Polybutylene Terphthalate
Polyethylene Terphthalate
Hexafluoroisopropanol + 0.075 M
Sodium Trifluoroacetate/55 C
or
m-Cresol + 0.05 m LiBr/100 C
Poly Acrylonitrile
ABS (Acrylonitrile Butadiene Styrene)
ASA (Acrylic Styrene Acrylonitrile
ABA (Acrylonitrile Butdiene Acrylate)
Carboxymethyl Cellulose
ABS/Polycarbonate
Polybutadiene Acrylonitrile
Polyurethane
DMF + 0.05 m LiBr/85 C
Polyacetal
Polyoxymethylene
DMF + 0.05 m LiBr/145 C
Polyimide
Polyamide imide
Polyetherimide
Polyethersulfone
Polyvinyldienefluoride
N-Methyl Pyrrolidone
+ 0.05 m LiBr/100 C
Polyfuran Formaldehyde
Dimethylacetamide/60 C
Shipping Solvent
Waters Styragel Columns

shipped in Toluene

shipped in THF

shipped in DMF
271
Styragel HR High-Resolution Columns

Designed particularly for low molecular weight samples, the Waters
Styragel HR ( high resolution) columns are ideal for t he analysis of
oligomers, epoxies, and polymer additives where high resolution is critical.
Packed with rigid 5 m particles, these columns deliver unrivaled resolution
and efficiency in the low-to-mid molecular weight region.
Calibration Curves for the Waters Styragel HR Series

of High-Resolution Columns
Styragel HR
Conditions:
Sample:
Mobile Phase:
Flow Rate:
Styragel HR High Resolution Columns for Unrivaled

Resolution of Low Molecular Weight Samples
Column Bank:
Mobile Phase:
Temperature:
Flow Rate:
Sample:
Styragel HR 0.5, 1, 2, and 3

THF
35 C
1 mL/min
Polystyrene standards:
0.5K, 5.05K, 49.8K
Conventional Styragel HR Columns

7.8 x 300 mm
Column Bank:
Mobile Phase:
Temperature:
Flow Rate:
Sample:
Styragel HR 0.5, 1, 2, and 3

THF
35 C
0.35 mL/min
Polystyrene standard mix
0.5K, 5.05K, 49.8K
Solvent-Efficient Styragel HR Columns

4.6 x 300 mm
Polystyrene
THF
1 mL/min
Elution Volume (mL)

Molecular Weight
Styragel HR 0.5
Styragel HR 1
Styragel HR 2
Styragel HR 3
Styragel HR 4
Styragel HR 5
Styragel HR 6
Styragel HR 4E
Styragel HR 5E
20
25
30
35
40
45 Min
20
25
30
35
40
45 Min
The 4.6 x 300 mm solvent-efficient Styragel columns deliver the same

high resolution as our conventional 7.8 x 300 mm Styragel columns, with
the added advantage of reducing solvent consumption by two-thirds.
Styragel HR Columns (7.8 mm i.d. x 300 mm)

Effective Molecular
Column
Styragel HR 0.5
Styragel HR 1
Styragel HR 2
Styragel HR 3
Styragel HR 4
Styragel HR 4E
Styragel HR 5
Styragel HR 5E
Styragel HR 6
Part No.
Weight Range
Part No.
THF
Part No.
DMF
Toluene
0-1,000
100-5,000
500-20,000
500-30,000
5,000-600,000
50-100,000
50,000-4 x 106
2,000-4 x 106
200,000-1 x 107
WAT044231
WAT044234
WAT044237
WAT044222
WAT044225
WAT044240
WAT054460
WAT044228
WAT054468
WAT044232
WAT044235
WAT044238
WAT044223
WAT044226
WAT044241
WAT054466
WAT044229
WAT054474
WAT044230
WAT044233
WAT044236
WAT044221
WAT044224
WAT044239
WAT054464
WAT044227
WAT054470
WAT054405
WAT054415
WAT054410
Styragel Guard Column 4.6 x 30 mm
Styragel HR Columns (4.6 mm i.d. x 300 mm)

T he same high performance as our conventional Styragel HR columns* with
the added advantage of reducing your solvent consumption by two-thirds.
Effective Molecular
Column
Styragel HR 0.5
Styragel HR 1
Styragel HR 2
Styragel HR 3
Styragel HR 4
Styragel HR 4E
Styragel HR 5E
272
Part No.
Weight Range
Part No.
THF
Part No.
DMF
Toluene
0-1,000
100-5,000
500-20,000
500-30,000
5,000-600,000
50-100,000
2,000-4 x 106
WAT045835
WAT045850
WAT045865
WAT045880
WAT045895
WAT045805
WAT045820
WAT045840
WAT045855
WAT045870
WAT045885
WAT045900
WAT045810
WAT045825
WAT045830
WAT045845
WAT045860
WAT045875
WAT045890
WAT045800
WAT045815
www.waters.com
Styragel HT High-Temperature, High-Stability Columns
Styragel HT Columns Deliver Superior Performance

Even at High Temperatures
Columns:
Mobile Phase:
Temperature:
Sample:
Calibration Curves for the Waters Styragel HT

Series of High-Temperature Columns
Styragel HT
Conditions:
Sample:
Mobile Phase:
Flow Rate:
Polystyrene
THF
1 mL/min
Elution Volume (mL)
Molecular Weight
T he Waters Styragel HT ( high temperature) columns can be used with

aggressive solvents at high temperatures without sacrificing resolution or
column lifetime. Packed with rigid 10 m particles, they have a typical
plate count greater than 10,000 plates per column, these columns are
extremely durable due to a narrow particle size distribution that results in
a very stable column bed. Suitable for both ambient and high-temperature
analysis, the Styragel HT columns offer excellent resolution of polymers in
the mid-to-high molecular weight range.
Styragel HT 2
Styragel HT 5
Styragel HT 3
Styragel HT 6
Styragel HT 4
Styragel HT 6E
Styragel HT 6E
TCB
140 C
LDPE
Styragel HT Columns (7.8 mm i.d. x 300 mm)

Effective Molecular
Column
Styragel HT 2
Styragel HT 3
Styragel HT 4
Styragel HT 5
Styragel HT 6
Styragel HT 6E
Part No.
Weight Range
Part No.
THF
Part No.
DMF
Toluene
100-10,000
500-30,000
5,000-600,000
50,000-4 x 106
200,000-1 x 107
5,000 - 1 x 107
WAT054475
WAT044207
WAT044210
WAT044213
WAT044216
WAT044219
WAT054480
WAT044208
WAT044211
WAT044214
WAT044217
WAT044220
WAT054476
WAT044206
WAT044209
WAT044212
WAT044215
WAT044218
WAT054405
WAT054415
WAT054410
Styragel HT Columns (4.6 mm i.d. x 300 mm)

T he same high performance as our conventional Styragel HT columns with
Effective Molecular
Column
Styragel HT 3
Styragel HT 4
Styragel HT 5
Styragel HT 6
Styragel HT 6E
www.waters.com
Part No.
Weight Range
Part No.
THF
Part No.
DMF
Toluene
500-30,000
5,000-600,000
50,000-4 x 106
200,000-1 x 107
5,000-1 x 107
WAT045920
WAT045935
WAT045950
WAT045965
WAT045980
WAT045925
WAT045940
WAT045955
WAT045970
WAT045985
WAT045915
WAT045930
WAT045945
WAT045960
WAT045975
273
Size excluSion chromatographygpc & gFc
Styragel HMW High Molecular Weight Columns

T he Styragel HMW ( high molecular weight) columns were specifically
designed for analysis of ultra-high molecular weight polymers susceptible
to shearing. Combining high-porosity 10 m frits and 20 m particles,
the Styragel HMW columns minimize polymer shear effects. T hese stateof-the-art columns can be used at either ambient or elevated temperatures
and exhibit excellent column lifetime.
Calibration Curves for the Waters Styragel HMW
Series of High Molecular Weight Columns
Styragel HMW
Conditions:
Sample:
Mobile Phase:
Flow Rate:
Column Bank:
Column Dimensions:
Polystyrene Stds:
Flow Rate:
Temperature:
Solvent:
2 Styragel HMW 7 and

2 Styragel HMW 6E
7.8 x 300 mm
14.4M, 3.84M, 330K,
34.5K, 3.25K
1 mL/min
145 C
TCB
Polystyrene
THF
1 mL/min
Column Bank:
Elution Volume (mL)
Molecular Weight
Styragel HMW Columns are Optimized for Analysis of

Shear-sensitive, Ultra-high Molecular Weight Polymers
Column Dimensions:
Polystyrene Stds:
Styragel HMW 2
Styragel HMW 6E
Flow Rate:
Temperature:
Solvent:
Styragel HMW 7
2 Styragel HMW 7 and

2 Styragel HMW 6E
7.8 x 300 mm
10.2M, 1.075M,
95K, 13K, 1.68K
1 mL/min
145 C
TCB
Styragel HMW Columns (7.8 mm i.d. x 300 mm)

Effective Molecular
Column
Styragel HMW 2
Styragel HMW 7
Styragel HMW 6E
Part No.
Weight Range
Part No.
THF
Part No.
DMF
Toluene
100-10,000
500,000-1 x 108
5,000-1 x 107
WAT054488
WAT044201
WAT044204
WAT054494
WAT044202
WAT044205
WAT054490
WAT044200
WAT044203
WAT054405
WAT054415
WAT054410
Part No.
THF
Part No.
DMF
Toluene
WAT046805
WAT046820
WAT046810
WAT046825
WAT046800
WAT046815
Styragel HMW Columns (4.6 mm i.d. x 300 mm)

T he same high performance as our conventional Styragel HMW columns with
Effective Molecular
Column
Styragel HMW 7
Styragel HMW 6E
Part No.
Weight Range
108
500,000-1 x
5,000-1 x 107
*System dead volume must be minimized for maximum column performance.
274
www.waters.com
Size excluSion chromatographygpc & gFc
HSPgel Columns for High Speed GPC Analysis

Waters HSPgel Column offering for high-speed GPC analysis, provides for
accurate and precise molecular weight determination, increased sample
throughput, and greatly reduced solvent consumption and disposal. Waters
offers four series of high speed GPC columns.
HSPgel HR series is used for high resolution,

room temperature GPC
HSPgel RT series for routine room temperature GPC
HSPgel HT series for high temperature GPC
HSPgel AQ series for aqueous SEC
T he HSPgel RT series are designed for room temperature, routine work of

organic polymer GPC. T hese come packed in THF and can be converted
multiple times from THF to toluene, chloroform, methylene chloride, DMF,
DMSO etc.
T he HSPgel HT series are designed for room temperature to high temperature (180 C) organic GPC. T he columns come shipped in either THF or
ODCB. T he ODCB packed column should be used for direct conversion to
TCB. T hese columns can withstand multiple solvent switches.
T he HSPgel AQ series are designed for room temperature analysis of water
soluble polymers.
T he column dimension for all columns is 6.0 x 150 mm.

T he HSPgel HR series is designed for high resolution, room temperature,
organic polymer GPC. T hese columns come packed in THF and can be
converted once to toluene, methylene chloride, or chloroform.
Name
Solvent
Particle Size
MW Range
Part No.
Ultra-High Resolution GPC

HSPgel HR 1.0
HSPgel HR 2.0
HSPgel HR 2.5
HSPgel HR 3.0
HSPgel HR 4.0
HSPgel HR MB-L
HSPgel HR MB-M
THF
THF
THF
THF
THF
THF
THF
3 m
3 m
3 m
3 m
3 m
3 m
3,5 m
100-1,000
500-10,000
1,000-20,000
2,000-60,000
10,000-400,000
500-700,000
1,000-4,000,000
186001741
186001742
186001743
186001744
186001745
186001746
186001747
THF
THF
THF
THF
THF
THF
THF
THF
THF
THF
THF
3 m
3 m
3 m
3 m
3 m
3 m
5 m
3 m
3 m
3 m
3,5 m
100-1,000
500-10,000
1,000-20,000
2,000-60,000
10,000-400,000
25,000-4,000,000
50,000-10,000,000
100-10,000
500-400,000
1,000-4,000,000
5,000->10,000,000
186001749
186001750
186001751
186001752
186001753
186001754
186001755
186001757
186001758
186001759
186001760
Water
Water
Water
Water
Water
Water
4 m
4 m
6 m
7 m
9 m
9 m
500-2,000
1,000-60,000
10,000-400,000
50,000-4,000,000
100,000->10,000,000
500-10,000,000
186001785
186001786
186001787
186001788
186001789
186001790
Aqueous GPC
HSPgel AQ 2.5
HSPgel AQ 3.0
HSPgel AQ 4.0
HSPgel AQ 5.0
HSPgel AQ 6.0
HSPgel AQ MB-H
Solvent
Particle Size
MW Range
Part No.
THF
THF
THF
THF
THF
THF
THF
THF
THF
THF
THF
THF
ODCB
ODCB
ODCB
ODCB
ODCB
ODCB
ODCB
ODCB
ODCB
ODCB
ODCB
ODCB
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
5 m
100-1,000
500-10,000
1,000-20,000
2,000-60,000
10,000-400,000
25,000-4,000,000
50,000-10,000,000
100,000->15,000,000
100-1,000
500-400,000
1,000-4,000,000
5,000->10,000,000
100-1,000
500-10,000
1,000-20,000
2,000-60,000
10,000-400,000
25,000-4,000,000
50,000-10,000,000
100,000->15,000,000
100-1,000
500-400,000
1,000-4,000,000
5,000->10,000,000
186001761
186001762
186001763
186001764
186001765
186001766
186001767
186001768
186001769
186001770
186001771
186001772
186001773
186001774
186001775
186001776
186001777
186001778
186001779
186001780
186001781
186001782
186001783
186001784
High-Temperature GPC
Room-Temperature GPC
HSPgel RT 1.0
HSPgel RT 2.0
HSPgel RT 2.5
HSPgel RT 3.0
HSPgel RT 4.0
HSPgel RT 5.0
HSPgel RT 6.0
HSPgel RT MB-L
HSPgel RT MB-L/M
HSPgel RT MB-M
HSPgel RT MB-H
Name
HSPgel HT 1.0
HSPgel HT 2.0
HSPgel HT 2.5
HSPgel HT 3.0
HSPgel HT 4.0
HSPgel HT 5.0
HSPgel HT 6.0
HSPgel HT 7.0
HSPgel HT MB-L
HSPgel HT MB-L/M
HSPgel HT MB-M
HSPgel HT MB-H
HSPgel HT 1.0
HSPgel HT 2.0
HSPgel HT 2.5
HSPgel HT 3.0
HSPgel HT 4.0
HSPgel HT 5.0
HSPgel HT 6.0
HSPgel HT 7.0
HSPgel HT MB-L
HSPgel HT MB-L/M
HSPgel HT MB-M
HSPgel HT MB-H
All columns are 6.0 x 150 mm
* MW ranges for HR and RT are based on polystyrene chain lengths, and

based on polyethylene oxide chain lengths for the AQ series.
** Exclusion limits for AQ series extrapolated from highest MW PEO standard, (~900,000).
www.waters.com
HR
High Resolution
MB
Mixed Bed
L/M
Low/Medium MW Range
RT
Room Temperature
Low MW Range
High MW Range
AQ
Aqueous
Medium MW Range
275
Shodex GPC Columns

High efficiency columns for GPC analysis. Featuring a very reproducible
styrene divinylbenzyne technology, Shodex GPC columns have more than
twenty years of history and have been used by customers all over the world.
Shodex GPC Columns

Type
K-800 Series (8 x 300 mm)
KF-800 (THF)
T hese are ultra-high efficiency columns designed for high resolution

performance. T hey are available in THF, DMF or c hloroform.
KF-801
KF-802
KF-802.5
KF-803
KF-804
KF-805
KF-807
KF-806M (linear)
KF-G pre-column (4.6 x 10 mm)
HFIP-800 Series (8 x 300 mm)

T hese columns have the same high efficiency as the K series columns
but are available in HFIP.
Polystyrene Exclusion Limit
Part No.
1.5 x 103
5 x 103
2 x 104
7 x 104
4 x 105
4 x 106
(2 x 108)
(4 x 107)
WAT030697
WAT030698
WAT030699
WAT034100
WAT034101
WAT034102
WAT034104
WAT034105
WAT034106
2 x 104
7 x 104
4 x 105
4 x 106
WAT034109
WAT034110
WAT034111
WAT034112
WAT035524
2.5 x 103
5 x 103
2 x 104
7 x 104
4 x 105
(4 x 107)
(2 x 108)
(4 x 107)
WAT034116
WAT034117
WAT034118
WAT034119
WAT034120
WAT034122
WAT034123
WAT034124
WAT034125
K-800 (Chloroform)
HFIP-800 Series (8 mm i.d. x 300 mm)

Type
Polystyrene Exclusion Limit
HFIP-803
HFIP-806M (linear)
HFP-LG precolumn (8 x 50 mm)
104
7x
(4 x 107)
Part No.
WAT035605
WAT035611
WAT035612
K-802.5
K-803
K-804
K-805
K-G precolumn (4.6 x 10 mm)
KD-800 (DMF)
KD-801
KD-802
KD-802.5
KD-803
KD-804
KD-806
KD-807
KD-806M (linear)
KD-G prec-olumn (4.6 x 10 mm)
276
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Envirogel High-Resolution GPC Cleanup Columns

T he Envirogel high-efficiency GPC cleanup columns are specifically
designed to remove low volatility, high molecular weight interferences, such
as lipids and natural resins, from environmental samples as specified in
EPA Method 3640A*. In the past, the cleanup procedure for environmental
samples was performed on low-efficiency GPC columns based on packing
particle diameters of 37-75 m (200 to 400 mesh) Bio-Beads S-X
resins. T he high-efficiency Envirogel GPC Cleanup columns increase the
speed of this process while simultaneously reducing solvent consumption.
Column Optimization
Column:
Sample:
Solvent:
Flow Rate:
Detection:
Two Envirogel GPC columns

19 x 300 mm and 19 x 150 mm
2000 L
Methylene Chloride
5 mL/min
UV at 254 nm, 1.5 AUFS
1.
2.
3.
4.
5.
Corn oil, 62 5 mg/mL

Bis(2-Ethylhexyl) Phthalate 2.5 mg/mL
Methoxychlor, 0.5 mg/mL
Perylene, 0.05 mg/mL
Sulfur, 0.2 mg/mL
Envirogel GPC Cleanup Columns

Packed in Methylene Chloride
Column
Envirogel GPC Cleanup
Envirogel GPC Guard
Dimensions
Part No.
19 x 150 mm
19 x 300 mm
4.6 x 30 mm
WAT036555
WAT036554
186001913
* EPA Method 3640A requires both columns.

For optimum capacity and resolution, a 150 mm column is used in series with the 300 mm column.
The use of both the 150 mm column and the 300 mm column provides maximum loading capacity while
the 300 mm column provides maximum throughput and reduction in solvent consumption when used alone.
Envirogel GPC Cleanup Columns

Packed in Cyclohexane/Ethyl Acetate
Column
Envirogel GPC Guard
Dimensions
Part No.
19 x 150 mm
19 x 300 mm
4.6 x 30 mm
186001915
186001916
186001914
* EPA Method 3640A requires both columns.
Preparative GPC Columns

Ultrastyragel Columns (19 mm i.d. x 300 mm)
For high resolution preparative applications, these columns are available
in toluene or THF.
Pore
Size
Effective Molecular
Weight Range
Flow Rate
mL/Min
Part No.
Toluene
Part No.
THF
50-1,500
100-10,000
200-30,000
5,000-600,000
50,000-4M
200,000-10M
2,000-4M
4-10
4-10
4-10
4-10
4-10
4-10
4-10
WAT025866
WAT025867
WAT025868
WAT025869
WAT025870
WAT025871
WAT025872
WAT025859
WAT025860
WAT025861
WAT025862
WAT025863
WAT025864
WAT025865
100
500
103
104
105
106
Linear
[Effective linear functional range: 2K-4M]
www.waters.com
277
Aqueous SEC Column Selection Guide
Eluent SelectionAqueous SEC with Ultrahydrogel Columns

Class
Polymer
Eluent
Nonionic
Polyethylene Oxide
Polyethylene Glycol
Polysaccharides
Pullulans
Dextrans
Cellulosics (H2O Soluble)
Polyvinyl Alcohol
Polyacrylamide
0.10M NaNO3
Nonionic Hydrophobic
Polyvinyl Pyrrolidone
80/20, 0.10M NaNO3/CH3CN
Anionic
Polyacrylic Acid (Na)

Polyalginic Acid (Na)
Hyaluronic Acid
Carrageenan
0.10M NaNO3
Anionic Hydrophobic
Polystyrene Sulfonate (Na) Lignin, Sulfonated
80/20, 0.10M NaNO3/CH3CN
Cationic
DEAE Dextran
Polyvinylamine
Polyepiamine
N-Acetylglucosamine
0.80M NaNO3
Cationic Hydrophobic
Amphoteric
278
0.10M TEA
0.10M TEA/1% HOAc
Polyethyleneimine
Poly (n-Methyl-2-Vinyl Pyridinium)
Lysozyme
Chitosan
Polylysine
Peptides
0.5M CH3COOH/0.3M Na2S04

0.50M HOAc/0.30M Na2SO4
5% NaH2PO4 with 3% CH3CN(pH 4.0)
0.10% TFA/40% CH3CN
Collagen/Gelatin
80/20, 0.10M NaNO3/CH3CN
0.50M NaOAc/0.50M HOAc
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Ultrahydrogel Columns
Packed with hydroxylated polymethacrylate-based gel, Waters Ultrahydrogel
SEC columns are ideal for the analysis of aqueous-soluble samples, such
as oligomers; oligosaccharides; polysaccharides; and cationic, anionic, and
amphoteric polymers. Measuring 7.8 x 300 mm, these high-resolution
columns offer many advantages over conventional aqueous SEC columns,
suc h as:
A wide pH range (2-12)
Compatibility with high concentrations of organic solvents

(up to 20% organic, 50% organic if the mobile phase is
introduced by gradient)
Greater flexibility for the mobile phase
Minimal non-size-exclusion effects
Ultra hydrogel Columns Calibration Curves

Column dimensions:
Sample:
Mobile phase:
Flow rate:
7.8 mm i.d. x 300 mm

PEG and PEO standards
Distilled water
1 mL/min
Gelatin Sample
Column:
Temperature:
Eluent:
Flow rate:
Detection:
Waters Ultrahydrogel 250,

500 and 1000
80 C
H2O, pH 7, phosphate buffer
1 mL/min
Waters 410 Differential
Refractometer
Ultra hydrogel Columns (7.8 x 300 mm)

Column
Pore Size
Exclusion Limit
Part No.
Ultrahydrogel 120
Ultrahydrogel 250
Ultrahydrogel 500
Ultrahydrogel 1000
Ultrahydrogel 2000
Ultrahydrogel Linear
Ultrahydrogel DP*
Ultrahydrogel Guard Column DP*
120
250
500
1000
2000
Blend
120
N/A
N/A
5 x 103
8 x 104
4 x 105
1 x 106
7 x 106
7 x 106
5 x 103
N/A
N/A
WAT011520
WAT011525
WAT011530
WAT011535
WAT011540
WAT011545
WAT011550
WAT011565
WAT011570
* DP = Degree of Polymerization, choice of column when working with glucose oligomers.
www.waters.com
279
Organic Calibration Standards
ReadyCal Polystyrene Standards
Organic Standards Kits

Qty./
MW
Description
Polystyrene Kit
Low-Mid MW*
102/10
102/10
Part No.
102/10
4x
g, 5.3 x
g, 9.5 x
g,
2.8 x 103/5 g, 6.4 x 103/5 g, 1 x 104/5 g,
1.7 x 104/5 g, 4.3 x 104/5 g,
1.1 x 105/5 g, 1.8 x 105/5 g
Polystyrene Kit
4.3 x 105/5 g, 7.8 x 105/5 g, 1.3 x 106/1 g,
Mid-High MW
2.8 x 106/1 g, 3.6 x 106/1 g, 4.3 x 106/1 g,
5.2 x 106/1 g, 6.2 x 106/1 g,
8.4 x 106/1 g, 2 x 107/1 g
Polystyrene Kit
5.8 x 102, 9.5 x 102, 1.2 x 103, 1.8 x 103,
SL-105
2.47 x 103, 3.77 x 103, 5.1 x 103,
7.6 x 103, 1.25 x 104, 1.7 x 104
Polystyrene Kit
1.2 x 103, 3.25 x 103, 1.02 x 104, 2.8 x 104,
SM-105
6.8 x 104, 1.95 x 105, 4.9 x 105, 1.08 x 106,
1.75 x 106, 2.75 x 106
Polystyrene Kit
4.5 x 105, 1.27 x 106, 2.3 x 106, 3.26 x 106,
SH-75
4.34 x 106, 8 x 106, 1.5 x 107
2.4 x 103, 9.5 x 103, 3.1 x 104,
Mid MW Kit
5.2 x 104, 1 x 105, 1.7 x 105, 2.7 x 105,
4.91 x 105, 7.3 x 105, 1 x 106
1 x 103, 1.7 x 103, 2.5 x 103,
Low MW Kit
3.5 x 103, 5 x 103, 7 x 103, 1 x 104,
1.3 x 104, 2 x 104, 3 x 104
Polybutadiene Kit
1 x 103, 3 x 103, 7 x 103, 1 x 104, 3 x 104,
7 x 104, 1 x 105, 3 x 105, 7 x 105, 1.1 x 106
Polyisoprene Kit
1 x 103, 3 x 103, 1 x 104, 3 x 104, 7 x 104,
1 x 105, 3 x 105, 5 x 105, 1 x 106, 3 x 106
WAT011588
WAT011610
500 mg/each
WAT034208
500 mg/each
WAT034209
500 mg/each
WAT034210
500 mg/each
WAT035706
500 mg/each
WAT035707
500 mg/each
WAT035709
500 mg/each
WAT035708
* Approximate molecular weights
Polystyrene (PS) Organic Standards (Individual Standard)

Approximate Molecular Weight Range
LS*
GPC
2.8 x 103
6.2 x 103
1.03 x 104
1.67 x 104
4.39 x 104
1.07 x 105
1.86 x 105
4.22 x 105
7.75 x 105
1.26 x 106
2.86 x 106
3.84 x 106
4.48 x 106
5.48 x 106
6.77 x 106
8.42 x 106
2.0 x 107
4 x 102
5.3 x 102
9.5 x 102
2.8 x 103
6.4 x 103
1.01 x 104
1.73 x 104
4.30 x 104
1.06 x 105
1.84 x 105
4.27 x 105
7.91 x 105
1.30 x 106
2.80 x 106
3.61 x 106
4.27 x 106
5.20 x 106
6.20 x 106
T hirty autosampler vials which contain 4 polystyrene standards per vial.

T here are three separate molecular weight ranges in each kit, 10 units of
each of the three molecular weight standards. Just add solvent to the vial,
let stand for two hours, shake gently and load into your autosampler for
analysis. Each kit comes with detailed instructions for proper usage.
Type
ReadyCal, 4 mL
Autosampler Vial
ReadyCal, 2 mL
Autosampler Vial
Approximate
Standards
Molecular Weight
Part No.
12
4 x 102 x 2 x 106
WAT058930
12
4 x 102 x 2 x 106
WAT058931
Aqueous Calibration Standards

Aqueous Standards Kits
Qty./
MW
Description
Part No.
* 5.8 x 103, 1.22 x 104, 2.37 x 104, 1 x 105,

1.86 x 105, 3.8 x 105, 8.53 x 105
Dextran Kit
5 x 103, 1.2 x 104, 2.4 x 104, 4.8 x 104,
1.48 x 105, 2.73 x 105, 4.1 x 105, 7.5 x 105
Polyethyleneoxide
2.4 x 4 x 104, 8 x 104, 1.6 x 105,
(PEO) Kit
3.4 x 105, 5.7 x 105, 8.5 x 105
Polyethylene Glycol
1 x 102, 2 x 102, 4 x 102, 6 x 102,
(PEG) Kit
1 x 103, 1.5 x 103, 4.3 x 103,
7 x 103, 1.3 x 104, 2.2 x 104
Polyacrylic Acid Kit
1 x 103, 3 x 103, 7 x 103, 1.5 x 104,
3 x 104, 7 x 104, 1 x 105,
3 x 105, 7 x 105, 1 x 106
Pullulan Kit
200 mg /each
WAT034207
500 mg/each
WAT054392
500 mg/each
WAT011572
1 gram/each
WAT035711
250 mg/each
WAT035714
* Approximate molecular weights

Qty.
Part No.
10 g
10 g
10 g
5g
5g
5g
5g
5g
5g
5g
5g
5g
1g
1g
1g
1g
1g
1g
1g
1g
WAT011590
WAT011592
WAT011594
WAT011596
WAT011598
WAT011600
WAT011602
WAT011604
WAT011606
WAT011608
WAT011612
WAT011614
WAT011616
WAT011618
WAT011620
WAT011622
WAT011624
WAT011626
WAT011628
WAT011630
Polyethyleneoxide (PEO) Aqueous Standards

Approximate Molecular Weight Range
LS*
GPC
Qty.
Part No.
2.5 x 104
4.0 x 104
7.3 x 104
1.5 x 105
2.8 x 105
6.6 x 105
8.5 x 105
0.5 g
0.5 g
0.5 g
0.5 g
0.5 g
0.5 g
0.5 g
WAT011574
WAT011576
WAT011578
WAT011580
WAT011582
WAT011584
WAT011586
2.4 x 104
4.0 x 104
7.9 x 104
1.6 x 105
3.4 x 105
5.7 x 105
8.5 x 105
* Light scattering
* Light scattering
280
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and T he Science of W hats possible are trademarks of Waters Corporation.
[ Waters Quality Parts and Supplies ]

Introduction. .............................................................................284
HPLC Pumps
ACQUITY UPLC System
515 HPLC Pump .....................................................296
ACQUITY UPLC Performance Maintenance Kits
1515 HPLC Pump ..................................................296
ACQUITY UPLC Detector PM Kits...........................285
1525 HPLC Pump ..................................................297
ACQUITY UPLC Binary Solvent Manager PM Kits 285

ACQUITY UPLC Sample Manager PM Kits.............285
1525 micro HPLC Pump ........................................297

Gradient Modules
ACQUITY UPLC Spare Parts & Tubing
2545/2525 Binary Gradient Module ..................298
Column Manager & Column Heater/Cooler .........285
2535 Quaternary Gradient Module .....................298
Binary Solvent Manager........................................285
Sample Manager ....................................................286

Column Heater .......................................................286

Fluid Handling Units
TUV Detector ..........................................................286
600E Fluid Handling Unit ....................................300
2996 Detector .......................................................286
626 LC Fluid Handling Units................................300
PDA Detector ..........................................................286
PrepLC 2000 Pump ................................................301
ELS Detector ...........................................................286

FLR Detector ...........................................................287
Reagent Manager ...................................................301

Injectors/Fraction Collectors
eLambda Detector ..................................................287
717plus Autosampler ...........................................302
Sample Organizer ..................................................287
2707 Autosampler................................................302
New ACQUITY UPLC H-Class System

Quaternary Solvent Manager ................................288
2757 Sample Manager/Fraction Collector ..........303
Sample Manager FTN .............................................288
2767 Sample Manager .........................................304
Column Heater Active ...........................................288
Waters Fraction Collector III ..................................304
UPLC Open Architecture System .............................................289
Manual Injectors.....................................................305
ACQUITY UPLC Local Console Controller Kit .........................289
2777/2777C Sample Manager ...........................305

nanoACQUITY UPLC PM Kits ...........................................290
nanoACQUITY UPLC Spare Parts & Tubing
High Pressure BSM/ASM ........................................290
High Pressure Sample Manager ............................291
TUV Detector ..........................................................291
with 2D Technology ...............................................291
ACQUITY UPLC FlexCarts .........................................................291
Separations Modules
2690/2695 Separations Module.........................292
2690D/2695D Separations Module ....................292
2796 Separations Module ....................................293
2790/2795 Separations Module.........................293
Important Information about Selecting Components
for Waters Separations Modules and Pumps ..........................294
Common Parts for Alliance Systems ......................................295
Common Parts for HPLC Pumps ......................................295
282
2747 Autosampler................................................303
Detectors
2487/2488 Dual-Wavelength
Absorbance Detector .............................................306
2489 UV/Visible Dual-Wavelength
Absorbance Detector .............................................306
2996 Photodiode Array Detector .........................307
2998 Photodiode Array Detector .........................307
2414 Refractive Index Detector ...........................309
2424/2420 Evaporative Light
Scattering (ELS) Detector.......................................309
2465 Electrochemical Detector ............................309
2475 Multi-Wavelength
Fluorescence Detector ............................................310
432 Conductivity Detector ...................................310
Other Systems
Alliance GPC/V 2000 System ..............................310
Mass Spectrometers
Quadrupole Time of Flight (Q-Tof) Mass Spectrometers
Q-Tof II ....................................................................311
Q-Tof Ultima API ....................................................311
Q-Tof micro.............................................................311
Q-Tof Premier .........................................................311
Q-Tof Premier MALDI .............................................311
SYNAPT MS/HDMS .................................................311
SYNAPT G2.............................................................311
Xevo QTof ...............................................................312
Xevo G2 QTof .........................................................312
Tandem Quadrupole Mass Spectrometers
Quattro Ultima .......................................................313
Quattro Ultima Pt ..................................................313
Quattro micro .........................................................313
Quattro micro GC ...................................................313
Quattro Premier/Premier XE ..................................314
TQ Detector.............................................................314
Xevo TQ ..................................................................315
Xevo TQ-S ...............................................................315
Single Quadrupole Mass Spectrometers
EMD 1000/ZQ........................................................316
3100 Mass Detector..............................................316
SQ Detector ............................................................316
Time of Flight (TOF) Mass Spectrometers
MALDI micro MX.....................................................317
LCT...........................................................................317
LCT Premier/Premier XE .........................................317
GCT ..........................................................................318
GCT Premier .........................................................318
Magnetic Sector Mass Spectrometer
AutoSpec/AutoSpec Premier .................................318
Waters Global Services ............................................................319
General HPLC Parts
Column and Cartridge Fittings
and Accessories ...............................................................320
SLIPFREE Connectors........................................................321
PEEK Tubing and Fittings ................................................322
PTFE/ETFE Tubing .............................................................323
Stainless Steel Tubing ....................................................323
Compression Screws and Ferrules ..................................323
Filters ...............................................................................323
283
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Waters knows how to run HPLC and LC/MS laboratories at peak
performance. Our instruments, software, c hemistries, and services
provide you with the tools you need to be successful.
Only Waters designed and manufactured Quality Parts have been tested
and certified for consistent optimal performance of your Waters systems.
By using Waters Quality Parts, you can be confident of consistent
instrument operation, accurate and reproducible results, and continued
system compliance.
Performance Maintenance Kits

All the parts you need, in one box
Waters Performance Maintenance Kits (PM Kits) are exclusively designed
to meet the needs of Waters instruments. Our PM and parts kits offer
you a cost-effective way to ensure t hat your Waters instrument is
properly maintained.
Each kit contains the Waters Quality Parts necessary to keep your instrument operating at peak performance. A detailed maintenance protocol,
including calibration and diagnostic tests, is included with eac h kit to
ensure proper installation and functionality. Youll also find an instrument
sticker that you can use to document when maintenance was performed,
and to help you remember when it will be required again.
To Find Parts:
Use Our Waters Quality Parts Locator
Visit Waters online at www.waters.com/parts to use the Waters Quality Parts
Locator, which allows you to locate, view, and order instrument components.
T he Waters Quality Parts Locator offers detailed information and schematics
for parts listed in this catalog. Simply select the appropriate instrument and
click on its image to drill down to identify the part youre looking for. Youll
also find troubleshooting information from our technical experts, to help
you determine if a part should be repaired or replaced.
The online Waters Quality Parts Locator is an easy way to

click through an instrument to find the parts you need.
To ensure the maximum performance and longevity of your instrument,

schedule a Performance Maintenance visit every 12 months.
Literature
References
Waters Global Services: W hy Choose Waters Services Brochure
284
Waters Quality Parts and Supplies
Ordering is Easy, Online or by Phone

Prices for Performance Maintenance Kits and Waters Quality Parts are
available in your currency by contacting your local Waters sales office. For
countries other than the U.S., please refer to our worldwide sales office list
on the back cover of this catalog for contact information. Local-currency
prices are also available to registered users at waters.com.
Online:
Go to www.waters.com/order
Telephone:
1-800-252-4752 (U.S. and Canada)
www.waters.com
Waters Quality Parts and suPPlies

ACQUITY UPLC System
ACQUITY UPLC Sample Manager Performance Maintenance Kit
Description
Part No.
ACQUITY UPLC Sample Manager Performance Maintenance Kit
201000174
PM Kit includes: Syringe, Needle, and Filters.
ACQUITY UPLC Column Manager and Column Heater/

Cooler Spare Parts & Tubings
Description
ACQUITY UPLC Detector Performance Maintenance Kits

Description
Part No.
ACQUITY UPLC 2996 PDA Detector Performance Maintenance Kit

(Through Serial Number: K05UPD699N)
ACQUITY UPLC TUV/PDA /eLambda Detector PM Kit
ACQUITY UPLC TUV Detector PM Kit (through SN: K05UPT699N)
ACQUITY UPLC ELS Detector PM Kit
ACQUITY UPLC FLR Detector PM Kit
WAT052586
201000186
WAS081142
201000159
201000193
ACQUITY UPLC Binary Solvent Manager Performance Maintenance Kit

Description
ACQUITY
Part No.
i2Valve
BSM Performance Maintenance Kit
201000197
PM Kit includes: Plungers, Check valves, Seals and Filters.
Description
ACQUITY UPLC Binary Solvent Manager Performance Maintenance Kit
Qualification/Calibration Kits
Designed to be used with Waters
Qualification Workbooks or Calibration
procedures. T hese specially-prepared
solutions come with a Material Safety
Data Sheet and a Certificate of Analysis
to assist you in successfully completing
your own qualification tasks.
For a complete product listing and pricing,
please visit Waters NEW Order Center
www.waters.com
205000291
205000365
205000478
410001905
700001012
700003114
700003139
700003147
700003151
700003156
700003164
700003167
700003168
700003169
700003170
ACQUITY UPLC Binary Solvent Manager Spare Parts & Tubings

Description
Parts and accessories

Assembly, acv actuator
Primary inlet cv filter kit, 2/pk
Gloves, nitrile, cr100, sterile, size 7
Gloves, nitrile, cr100, sterile, size 9
Assembly, check valve, double ball and seat
Assembly, tube, transducer to check valve
Assembly, tube, ssv to active check valve
Fuse, 0.5 A slow blow
Part No.
Column Stabilizer Kit, 50/100mm Columns

Column Stabilizer Kit, 150mm Columns
Column Support Clips, 10/pk
1-piece Fitting, 10-32, 10/pk
PEEK Tubing Cutter
Ferrule, PEEK, 1/16, HPFT, 10/pk
Fingertight Reusable Fittings Kit
Door Seal Gasket
Snap-in Clip, 1/16 Tubing
Column Retainer Rod, 2/pk
Exit Drip Tray
I-Button Cord Clip
Collet, Reusable, HPFT, 10/pk
Compression Screw, Reusable, HPFT, Gold, 10/pk
Collet & Compression Screw Multi-Tool
700003557
700002912
700002964
700002965
700002968
430001773
430001443
WAT042091
Part No.
201000173
Part No.
ACQUITY Binary Solvent Manager PM Kit

100 L Filter Mixer
High Sensitivity Filter Mixer, 425 L
Tube Assembly, Solvent Inlet SDS
Degasser Port B2 to SSV B Tube Assembly
Tube Assembly, Transducer to Check Valve
Vent Valve P5 to Tee/Filter Tube Assembly
Tube Assembly, System Outlet SDS
Transducer Assembly, Head Mounted, 15K psi
15K psi Head
UPLC Primary Check Valve Assembly, 2/pk
Seal Wash Housing
Wash Seal, 2/pk
Head Plunger Seal
Plunger, 2/pk
Support Plate, Thickened, VHP Head
Solenoid Valve, Solvent Select
Fuse, 5A, 250V, 5 x 20 mm, SLO BLO, 5/pk
1/4in. Short compression screw (Gold-Plated)
Ferrule Set, 1/16 in ID, Two-piece, 10/pk
Union, .020 ID
Screw, Compression, Long, GOLD, 10/pk
Vent Valve/Trap Valve Catridge
Solvent Bottle Filter, Stainless Steel, 7/pk
Solvent Bottle Filter, Stainless Steel, 1/pk
50 L High Pressure Filter Mixer
Primary Inlet Check Valve Filter Kit, 2/pk
ACQUITY Accum. Check Valve, 2/pk
Solvent Filter
Compression Screw, 1/8, PEEK, 5/pk
Tefzel Ferrules, 1/8i n., yellow, 5/pk
O-Ring, Teflon
201000173
205000404
205000403
430001020
430001113
430001121
430001207
430001486
700002594
700002595
700002596
700002597
700002598
700002599
700002600
700002601
700002603
700002604
700002634
700002635
700002636
700002645
700002660
700003616
700003615
700002911
700002912
700002968
WAT025531
WAT037372
WAT037373
WAT076152
285
ACQUITY UPLC Sample Manager Spare Parts & Tubings

Description
ACQUITY UPLC 2996 Detector Spare Parts & Tubings

Part No.
ACQUITY Sample Manager PM Kit

ACQUITY UPLC Column In-Line Filter Kit
Needle Stainless Steel, 30 L
Needle, 15 L Stainless Steel
Needle, Stainless Steel Tip, 30 L
Needle, Stainless Steel Tip, 15 L
Tube Holder, 24-well, 1.5mL Tubes
Tube Holder, 48-well, 0.65 mL tubes
Vial Holder, 24-well, 4 mL vial
Vial Holder, 48-well,2 mL vial
250L Sample Syringe
Sample Needle Fitting Kit
Tube Assembly, Inject Outlet, (UPLC fittings both ends)
Needle Guide Tube
Tube Assembly, Inject Out, (UPLC fitting at injector& HPLC fitting at Col. Inlet)
Sample Loop, 2 L
Sample Loop, 5 L
Sample Loop, 20 L, std.
Sample Loop, 50 L
Sample Loop, 10 L
2.5 mL Wash Syringe
100 L Sample Syringe
Needle Seal O-ring, 002 Kalrez
Needle Assembly, PEEK
ACQUITY Injector POD/Cartridge
0.2 m SS Column In-Line Replacement Frits, 5/pk
201000174
205000343
205000362
205000363
205000369
205000370
405003740
405003741
405003742
405003743
410001347
410001659
430001084
430001086
430001221
430001264
430001311
430001320
430001325
430001326
700002569
700002570
700002572
700002644
700002765
700002775
ACQUITY UPLC Column Heater Module Spare Parts & Tubings

Description
Part No.
Flow Cell, Std., ACQUITY 2996 (For 2996 S/N K05UPD699 or lower)
Flow Cell, Low Flow., ACQUITY 2996 (For 2996 S/N K05UPD699N or lower)
Connector Plug, 10-position
Tube Assembly, Low Flow, 2996 Inlet
Tube Assembly, Std. Flow, 2996 Inlet
Capillary Tubing Cutter
PEEK Tubing Cutter
Backpressure Regulator
ACQUITY 2996 PM Kit ( For 2996 w/ S/N K05UPD699N or lower)
FUSE, 3.15A ( 250V, 5 x 20 mm), 2/pk
I/O Terminal Plug, 10-pin
Power Cord, 110V
Fuse 2.0A, 250V, Fast-Blo, 5 x 20 mm
Lamp Mounting Screw, M4 x 10
205015002
205015003
323000247
430001783
430001784
605000101
700001012
700002676
WAT052586
700001800
WAT057112
WAT097194
WAT163-14
WAT411410
ACQUITY UPLC PDA Detector Spare Parts & Tubings

Description
Part No.
ACQUITY TUV/PDA PM Kit (For PDA w S/N K05UPD700N or higher)

ACQUITY UPLC ID Cell PDA, Analytical Flow Cell
ACQUITY UPLC ID Cell PDA, High Sensitivity
Assembly, Tube Inlet .004 ID LT PEEK Nut PDA
Ethernet Patch Cord, 5ft.
PEEK Tubing Cutter
Power Cord, 110V
201000186
205015017
205015019
323000247
430001784
441000456
605000101
700001012
700002676
WAT097194
Part No.
Column Support Clips, Column Heater, 10/pk

Column Stabilizer Kit, 50/100 mm Columns
Column Stabilizer Kit, 150 mm columns
Column Heater Thermal Gasket
Front Cover, Column Heater
Ferrule, PEEK, 1/16, HPFT, 10/pk
Collet, Reusable, HPFT, 2/pk
Comp. Screw, Reusable, HPFT, Gold, 2/pk
205000263
205000291
205000365
425000536
700002587
700003114
700003115
700003116
ACQUITY UPLC TUV Detector Spare Parts & Tubings

Description
Part No.
ACQUITY UPLC TUV/PDA PM Kit (TUV w/ S/N K05UPT700N or higher)

Flowcell, Std., ACQUITY TUV (for TUV w/ S/N K05UPT699N or lower)
Flowcell, Low Flow, ACQUITY TUV (for TUV w/ S/N K05UPT699N or lower)
ACQUITY UPLC ID Cell TUV, Analytical
Flowcell, High Sensitivity, 2.4 L Vol. (TUV w/ S/N K05UPT700N or higher)
Tube Assembly, Low Flow, TUV inlet
Tube Assembly, STd. Flow, TUV inlet
PEEK Tubing Cutter
Fuse, 3.15A ( 250V, 5 x 20 mm), 2/pk
2487/2488/ACQUITY TUV PM Kit (for ACQUITY TUV through S/N K05UPT699N)
Fuse 3.15A, 250V, 5 x 20 mm, 2/pk
Power Cord, 110V
286
Description
201000186
205015000
205015001
205015016
205015009
323000247
430001749
430001748
441000456
605000101
700001012
700001800
700002676
WAS081142
700001800
WAT097194
ACQUITY UPLC ELS Detector Spare Parts & Tubings

Description
Part No.
ACQUITY ELSD/ELSD Performance Maintenance Kit

Nebulizer
Packing Ring, Nebulizer
PEEK Tubing, 6 in. X .004 in. ID
PEEK Tubing, 14 in. X .004 in. ID
Ethernet Cross-over Cable, 3ft.
Bottle/Vapor Trap, 1000 mL
Siphon Drain Tube
Event In/Out Cable Assembly
Analog Out Cable Assembly
Fuse 5.0A, 250V, 5 x 20 mm, Fast-Acting
201000159
205000342
323000247
425000326
430001562
430001565
440000145
441000456
700002682
700002801
WAT020321
WAT057235
WAT163-18
Literature
References
Waters Educational Services Course Catalog,
www.waters.com/courses
Connections INSIGHT - Remote Services

Frequently Asked Questions
www.waters.com
ACQUITY UPLC FLR Detector Spare Parts & Tubings

Description
ACQUITY FLR Flow Cell Assembly
Fuse Drawer
Fuse 3.15A, 250V
10 position I/O Connector
Connector Shell Cover
Union, Internal Reducer
Fluorescence System PQ Solution
Analog out Cable Assembly
Power Cord, 110V
External ethernet cable
Part No.
700003711
WAT055426
700001800
323000247
323000446
410002096
700002676
700003694
WAT057235
WAT097194
441000372
ACQUITY UPLC eLambda Detector Spare Parts & Tubings

Description
ACQUITY UPLC ID Cell PDA, Analytical FC
ACQUITY UPLC ID Cell PDA, High Sensitivity
Ethernet Patch Cord, shielded, 5 ft.
Event Cable, 6 ft
Backpressure Regulator, 250 psi
Main Fuse, 3.15A, 250V, 5/pk
Power Cord, 110V
Cable Assembly, Shield Output
inlet tube assembly
leak sensor
Tube Assembly, low flow, PDA inlet
Part No.
205015017
205015019
323000247
441000456
441000373
700002676
700001800
WAT097194
WAT057235
430001784
205000505
430001783
NEW Waters High-Brightness Lamp

with Intelligent Technology
T he Waters High Brightness (HB) Lamp consists of characteristics
that go beyond what the competition can offer. T he HB lamp is
equipped with intelligent tec hnology when installed.
T his intelligent tec hnology allows for the electronic recording of
pertinent component information viewable within the Empower
Software database and is available for inclusion in a comprehensive
status report. It holds lamp changes and updates in a lamp history
file, including serial number, usage hours, and ignitions, so if the
lamp is transferred between units, the data travels with it.
No more guess work as to how long the lamp has been running or
the need to manually record the lamp c hanges its automatic.
Providing better traceability and accurate lamp lifetime recording
to our customers are just a few of the enhancements we have added
to this lamp.
T he High-Brightness Lamp with Intelligent Technology is currently
available for our latest ACQUITY UPLC PDA and TUV detectors.
ACQUITY UPLC Sample Organizer Spare Parts

Description
ACQUITY Sample Organizer PM Kit
Door Window Shade
Vial Holder, 2-mL vial (48-well)
Vial Holder, 1.5mL tubes (24-well)
Vial Holder, 0.65mL tubes (48-well)
Vial Holder, 4 mL vial (24-well)
Part No.
201000208
700003794
405003743
405003740
405003741
405003742

Designed to provide new, existing and potential UPLC users the ability to
understand how UPLC technology works, how to be successful with it, and
how it can provide impactful results within organizations (56 pages).
Quest for Ultra Performance

in Liquid Chromatography
Presents a brief review of the origin and history of LC, showing how
early the concepts of ultra performance were recognized and how
many decades it took to reduce them to practice (58 pages).
Description
www.waters.com
Description
Part No.
Performance Plus HB Deuterium Lamp Assembly
700005269

T his technology primer is designed to provide the reader with the
chromatography by understanding how the technique works, the
parameters that impact retention and selectivity, as well as the practical considerations necessary to successfully implement HILIC within a
chromatographic strategy.
Part No.
715002099
715002098
715002531
287

NEW ACQUITY UPLC H-Class System
Quaternary Solvent Manager Spare Parts & Tubing

Description
Sample Manager FTN Spare Parts & Tubing

Part No.
ACQUITY H-Class Quaternary Solvent Manager Performance Maintenance Kit

Bottle Tray
Seal Wash Waste Fittings
Plunger Removal Tool
Inlet manifold
Screw, Metric Skt Cap M5 x 40, 2/pk
Tube Assembly, Degasser to GPV
Tube Assembly, Transducer to Vent Valve
Tube Assembly, Vent Valve P4 to Waste
Tube Assembly, Vent Valve P2 to Outlet Filter
Tube Assembly, Transducer to Check Valve
Tube Assembly, GPV-D to Mixer Manifold
Tube Assembly, GPV-C to Mixer Manifold
Tube Assembly, GPV-A to Mixer Manifold
Tube Assembly, GPV-B to Mixer Manifold
Tube Assembly, Mixer Manifold to I2V
Outlet Housing Cartridge, Stainless Steel
Transducer
Pump Head
Seal Wash Housing
Seal Wash Housing Seal
Head Plunger Seal
Plunger
Head Support Plate
Ferrule Set (.062 2-piece), 10/pk
Vent Valve Cartridge Assembly
Screw, Metric Skt Cap M3 x 16, 4/pk
I2V Valve
Check Valve, Double Ball and Seat
I2V Valve Cartridge, 1/pk
Filter, Air, Door
Filter for GPV, 4/pk
Assy, Mixer, 100 L, QSM
O-ring, Teflon, Pump Head
Seal Wash Pump Solenoid
201000233
289002414
405003015
405007627
405010508
410001296
430002208
430002316
430002317
430002319
430002357
430002387
430002388
430002389
430002390
430002400
700001530
700002594
700002595
700002597
700002598
700002599
700002600
700002601
700002635
700002660
700004023
700005162
700005164
700005165
700005167
700005173
700005119
WAT076152
WAT270926
Description
ACQUITY H-Class Sample Manager- Flow Through Needle
Performance Maintenance Kit
Sample Needle Kit, 15 L
Inline Waste Valve, 1/4-28 thread
Seal Extension Tube
Injector Valve Cartridge, SM-FTN
Sample Needle Guide
Needle Seat
Syringe 100 L, HP
Part No.
201000234
700005215
410003180
700005234
700005236
405008854
405011492
700002570
Column Heater Active Spare Parts & Tubing

Description
Door Latch with Pins
Clip Retainer
Active Preheater Assembly
Column Support Clips, 10/pk
Screw, Panel, M4 x 16, Blue
Extension Arm Kit, Optional
Drip Tray, CH-A
Tygon Tubing (0.375 OD x 0.250 ID)
External Cable, Right Angle
I-Button, CH-A
Part No.
700005248
415001544
205000730
205000478
410003295
205000726
415001608
700001796
441001040
700005251
Description
ACQUITY H-Class Local Console Controller*
ACQUITY H-Class LCC Bracket*
Part No.
725000464
205000650
*Note: Both part numbers are required to properly install device to ACQUITY H-Class.
288
www.waters.com

UPLC Open Architecture System
UPLC Open Architecture System Spare Parts & Tubing
Description
Part No.
Open Archtecture UPLC Performance Maintenance Kit

10 L Sample Loop
25 L Syringe
5 L Sample Loop
Column Manager Tubing Assembly
Column Stabilizer Tubing Assembly
Injection Valve Adapter
Injection Valve Pod
MS Inlet Tubing Assembly
O-Ring, Injection Valve Drive
Syringe Kit, 25 L
System Outlet Tubing
Valve Drive
Waste Check Valve Kit
Ferrule Set, (062), 2-piece
Gold Compression Screw, Long, 10/pk
Gold Compression Screw, Short, 10/pk
Open Archtecture UPLC PM Kit
201000198
430001326
700002705
430001311
430002015
205000585
700004145
700004144
430001229
700004147
205000275
430001017
700002455
700004057
700002635
700002645
700002634
201000198
Literature
References
Connections INSIGHT Case Study: Reducing Instrument DownTime in an Analytical Laboratory
Literature Reference: 720001602EN
www.waters.com/insight
ACQUITY UPLC Local Console Controller Kit

Instant, convenient access to your ACQUITY UPLC System
ACQUITY UPLC Local Console Controller (LCC) lets you prepare systems for
operation, define initial conditions, and run diagnostic tests via a PDA-like
handheld device.

Simply prepare ACQUITY UPLC Systems for operation
Rapidly set up initial conditions
Easily view system status when

acquiring data
Quickly run diagnostic tests next

to the ACQUITY UPLC System
To learn more, go to www.waters.com/lcc
Description
Part No.
ACQUITY Local Console Controller Kit*

ACQUITY UPLC Local Console Controller Y-Cable & Power Supply Kit*
725000464
205000590
*Note: Part Numbers 725000464, 205000590 must be ordered to properly

install the device to the classic ACQUITY UPLC.
www.waters.com
289

nanoACQUITY High Pressure BSM/ASM Spares & Tubings
Description
nanoACQUITY UPLC Performance Maintenance Kits

Description
Part No.
nanoACQUITY UPLC TUV Detector Performance Maintenance Kit

nanoACQUITY UPLC Sample Manager Performance Maintenance Kit
ACQUITY UPLC TUV Detector Performance Maintenance Kit
(Systems with S/N K05UPT700N and above)
WAS081142
201000182
201000186
PM Kit includes: Syringe, Needle, and Filters.

nanoACQUITY UPLC Binary and Auxiliary Solvent
Manager Performance Maintenance Kit
201000181

ACQUITY UPLC Bottle Accessory Kit
205000589
ACQUITY UPLC Bottle Accessory Kit
Part No.
nanoACQUITY Solvent Manager PM Kit

Assembly, Bottle Tray
1/2 m Filter Assembly
Solvent Filter Assembly, 5 m
Degasser Port B2 to SSV B Tube Assembly.
Degasser Port B1 to SSV B Tube Assembly.
Degasser Port A1 to SSV A Tube Assembly.
Degasser Port A2 to SSV A tube Assembly.
Tube Assembly., Pumphead to Transducer
Tube Assembly., Transducer to Check Valve
Tube, Vent Valve port 1 to Waste
Tube, Vent Valve port 4 to Waste
Tube, Vent Valve Port 5 to Filter
Solvent Inlet Tube (Aux. Pump Only)
Tube Set, A2 & B2 Inlet Lines
Tube, Acc. A Transducer to Vent Valve P3
Tube, Acc. B Transducer to Vent Valve P6
Tube, Filter to Flow Sensor Inlet A
Tube, Filter to Flow Sensor Inlet B
Tube, ASM to MS, 25 m x 60in.
Fuse Drawer
Transducer Assembly., Head Mounted, 15K psi
15K Pump Head
UPLC Primary Check Valve Assembly., 2/pk
Wash Seal, 2/pk
Head Plunger Seal
I/O Connector Plug, 12 pin
Plunger, 2/pk
Support Plate, thickened
Solenoid Valve, Solvent Select
Fuse, 5A, 250V, 5 x 20 mm, SLO BLO, 5/pk
Ferrule Set, 1/16 ID, Two-piece, 10/pk
Screw, Comp., 10-32, SS Gold plated, 10/pk
Vent Valve/Trap Valve Cartridge
1/2 m Filter Insert Assembly
Tube, Vent Valve port 2 to Filter
Tube, ASM to MS, 25 m x 60 in.
Solvent Inlet System Tube Set
Check Valve, Double Ball and Seat, 2/pk
O-Ring, Teflon
201000181
289002414
289002111
289002172
430001113
430001114
430001115
430001116
430001120
430001121
430001209
430001210
430001346
430001389
430001436
430001511
430001512
430001534
430001535
430001568
430001569
430001572
700001502
700002594
700002595
700002596
700002598
700002599
WAT270868
700002600
700002601
700002603
700002604
700002635
700002645
700002660
700002696
700002702
700002712
700002713
700002968
WAT076152
WAT270868
For ACQUITY UPLC Columns and Accessories see Page 87
To ensure the maximum performance and longevity of your ACQUITY UPLC System, it is critical
that you use Waters Quality Parts for maintenance. For more information on parts and accessories
for your ACQUITY & nanoACQUITY UPLC Systems, please visit www.waters.com/parts
290
www.waters.com
nanoACQUITY High Pressure Sample Manager Spares & Tubings

Description
nanoACQUITY Sample Manager PM Kit
Shuttle Tray Kit
Kit, Tubing 2D/2 Pmp. Trap, nanoACQUITY
Union, Zero Dead Volume
nano-Tee without gauge pin
nano Trap Column Holder
Tube Holder, 24-Well, 1.5 mL Tubes
Tube Holder, 48-well, 0.65 mL Tubes
Vial Holder, 24-Well, 4 mL vial
Vial Holder, 48-Well, 2 mL vial
Fitting, Plug, PEEK, Knurled
Fitting, PEEK, Knurled one-piece
50 L Sample Syringe
2 L Sample Loop
5 L Sample Loop
20 L Sample Loop
50 L Sample Loop
10 L Sample Loop
Assembly, Cap. Tube with Frit 25 X 18 in.
Capillary Tubing w/frit, 25 m x 10 in.
Capillary Tubing w/frit, 40 m x 16 in.
25 m Capillary, BSM to Trap Valve
40 m Capillary, ASM to Inject Valve
Cap. Tubing Assembly., Inj. Valve to column
Cap. Tubing Assembly., Inj. Valve to Trap valve
3/16 in. Open End Wrench
2.5 mL Wash Syringe
100 L Sample Syringe
Needle Seal O-ring, 002 Kalrez
Fuse, 0.25A, 250V
Fuse, 10A, 5 mm x 20 mm, Slo Blo
1/4 in. Short Comp. Screw (Gold-plated)
Sample Needle, PEEK, 15 L
Capillary Tube, 300 m Column Inlet
Capillary Tube, 300 m Column outlet
300 m Column Inlet/Outlet Tubing Kit
Injector Valve POD/Cartridge
Column Heater
Part No.
201000182
205000542
205000398
289000439
289002576
289002802
405003740
405003741
405003742
405003743
405005067
405005068
410001348
430001264
430001311
430001320
430001325
430001326
430002242
430001570
430001571
430001575
430001576
430001577
430001629
700000610
700002569
700002570
700002572
700002576
700002577
700002634
700002708
700002754
700002755
700002757
700002907
700002908
nanoACQUITY UPLC System with 2D Technology Spare Parts & Tubings

Description
Part No.
Assembly, Waste Tube, HTM, Peek

Union, Nano, 6-40
Tee, Nano M-M-V Detail
Cap Tubing, BSM-mmv Tee, M-M 25 x 24 in.
Cap Tubing, inj vlv-trp vlv, V-V 40 x 13 in.
Holder, MVM nano Tee
Assembly, Cap Tubing, 1st D Col. in/out 10 in.
Assembly, Cap Tubing, Trap vlv-MMV tee 12 in.
Assembly, cap tubing, BSM2-inj vlv, 22 in.
Assembly, Cap Tubing, BSM-Trap vlv, 30 in.
Assembly, POD, trap valve, 3 trace
Assembly, Cap. Tube with Frit 25 u x 18 in.
nanoACQUITY Trap Sym C18 5 m 180 m x 20 v/v
Nano-Tee , M-M-M Detail
Pin Plug, 1/16 in., High Press.
Cap. Tubing Assembly, Injection Valve to Trap Valve
Cap. Tubing with Frit, 40 m x 16 in.
Tubing Assembly, Solvent Select Valve to Inline Filter
Mixer Assembly, 1.0 x 50 mm, Zirc. Bead
Tubing, Capillary, 300 m Col. Outlet
Tubing, Capillary with Frit, 75 m x 10 in.
Tubing, Capillary with Frit, 75 m x 8 in.
Thumb Screw, Stainless Steel, M5 x 11 mm Large
Ethernet Switch Box, 8-port
Mixer Kit for 1 mm column
430001456
289004407
289004442
430002140
430001629
700004599
430002183
430002153
430002155
430001575
700004601
430002242
186004630
289002576
700002747
430001577
430001571
430001470
289003345
430001848
430001837
430001835
410001697
725000455
205000540
ACQUITY UPLC FlexCarts

Make your system mobile
T he ACQUIT Y UPLC FlexCart wheeled cart is a complete system
platform that facilitates convenient installation of the ACQUITY UPLC
and makes it easier to position the system close to the inlet of the
mass spectrometer. T he FlexCart provides electrical outlets, supports
computer monitor and keypad, and manages fluid waste container. The
FlexCart is available for both ACQUITY UPLC and nanoACQUITY Systems.
nanoACQUITY TUV Detector Spares & Tubings

Description
ACQUITY UPLC TUV PM Kit (TUV w/ S/N K05UPT700N and above)
TUV Flow Cell, 10 nL (TUV w/ S/N through K05UPT699N)
TUV Flow Cell, 10 nL (TUV w/ S/N K05UPT700N and above)
Connector Plug, 10 position
Fuse, 3.15A (250V, 5 x 20mm), 2/pk
UPLC TUV Detector PM Kit (TUV w/ S/N through K05UPT699N)
Fuse Holder
Fuse, 3.15A, 250V, 2/pk
Part No.
201000186
205000159
205015013
323000247
700001800
700002676
WAS081142
WAT055426
700001800
ACQUITY UPLC FlexCart

Part No. 205015015
Description
Part No.
ACQUITY UPLC FlexCart

nanoACQUITY UPLC Flex Cart
www.waters.com
205015015
205016040
291

Separations Modules
2690/2695 Separations Module
Description
Part No.
2690/2695 Separations Module Performance Maintenance Kit
WAT270944
PM Kit includes: Plungers, Check valves, Seals, Filters, Needle,

Syringe (250 L), and Injector Rebuild Kit.
Parts & Accessories
HPMV tool kit
Solvent bottle caps, 4 L (4/pk)
Bottle caps, 1 L (4/pk)
Plunger seals replacement kit, black (2/pk)
Replacement plunger
Syringe, 2500 L
Syringe, 25 L
High-pressure motor valve rebuild kit
WAT045427
WAT062341
WAT062479
WAT271066
WAT271067
WAT077342
WAT077343
WAT045424
Replacement Plunger WAT271067
2690/2695 Performance Maintenance Kit
2690D/2695D Separations Module*
Description
Part No.
2690D/2695D Separations Module 8 Needle Performance Maintenance Kit
700000201
PM Kit includes: Filters and Syringe (2.5 mL), and Tubing Kit.
Parts & Accessories
HPMV tool kit
Solvent bottle caps, 4 L (4/pk)
Bottle caps, 1 L (4/pk)
Plunger seals replacement kit, black (2/pk)
Replacement plunger
Syringe, 2500 L
Syringe, 25 L
High-pressure motor valve rebuild kit
WAT045427
WAT062341
WAT062479
WAT271066
WAT271067
WAT077342
WAT077343
WAT045424
* Note: For proper maintenance of the 2690D/2695D, please make sure to order PM Kit
700000201 and PM Kit WAT270944.
2690D/2695D Separations Module 8 Needle

292
www.waters.com
2796 Separations Module
Description
Part No.
2796 Separations Module Performance Maintenance Kit
201000169

Parts & Accessories
Sample Loop, 5 L, PEEK
Sample Loop, 2 mL, PEEK
430000781
430000762
430000782
430000783
430000784
430000785
2790/2795 Separations Module
Description
Part No.
2790/2795 Separations Module Performance Maintenance Kit
201000107

Parts & Accessories
Syringe, 25 L
Syringe, 250 L
Syringe, 2500 L
WAT077343
WAT073109
WAT077342
Syringe
Literature
References
Waters Educational
Services Course Catalog,
www.waters.com
Total Assurance Warranty Brochure,

293
Important Information about Selecting Components

for Waters Separations Modules and Pumps
Waters Separations Modules and Pumps are assembled using the highest
quality components available. T he parts for Performance Maintenance that
have been recommended are intended to optimize the system for the widest
variety of applications.
A number of options are available for applications that may require
alternative technology to meet different performance expectations. In some
cases, these options may provide better performance for a particular
application and operating environment. T he following may be used as a
guide to selecting alternative components.
T his information is not intended as a hard-and-fast set of rules, but as
recommendations that may be more effective depending upon your specific
application requirements. Some experimentation may be required to find
the best configuration for your application.
Sapphire plungers
One of the most important components is the sapphire plunger. It must be
clean and smooth if any plunger seal is expected to work for any length
of time. Waters recommends in our Performance Maintenance strategy that
you change them once each year.
Waters offers two plungers. T he standard plunger is manufactured using
the traditional processes in place by Waters and other instrument vendors.
Waters also offers the PerformancePLUS Sapphire Plunger, whic h has
the crystalline structure of the plunger oriented lengthwise, rather than
randomly. T his results in a harder surface around the circumference
of the plunger, extending its expected lifetime. T he condition of the
sapphire plungers is critical. PerformancePLUS plungers with the oriented
crystal structure will provide the best sealing surface and durability.
Plunger seals
Plunger seal material is another critical variable. Plunger seals are available in several materials that can be used for improved performance in
specific applications. Plunger seal life should typically be six months to
one year; you may try the alternative seals if the lifetime is lower.
Plunger seal wash

Seal-wash solvent lubricates the plungers and flushes away any solvent or
dried salts forced past the plunger seal from the high-pressure side of the
piston chamber. T his wash cycle extends the life of the seals. Position the
plunger seal-wash reservoir in a visible location above the solvent management system, and refill the reservoir as necessary with a solvent that is
suited to your application.
294
For reversed-phase HPLC applications, use an aqueous plunger seal-wash

solution with enough organic content to inhibit bacterial growth. For
example, use an 80 percent water with 20 percent methanol or acetonitrile,
depending on your application. For all GPC (normal phase) separations, use
a 50/50 mix of methanol and water for your seal-wash solution.
Note: Please ensure that you use separate solutions and containers for the
plunger-seal wash and the sample management system or autosampler
needle wash. Because the functions of these solutions differ, the use of
one solution for both functions may compromise the effectiveness of either
needle washing or plunger-seal washing. Change plunger seal wash seals
whenever the main plunger seals are changed.
Check Valves
Check valve failures can be a common cause of reproducibility problems.
T here are a number of check valve failure modes, including sticking (failure
to open or close) and intermittent leaks, which results in variable retention
times and pressure fluctuations. Intermittent leaks are often caused by
particulate matter that sticks to the c heck valve ball. T his material can
come from the mobile phase, shedded seal material, dirty glassware, or
poor laboratory cleanliness.
Waters offers optional configurations of check valve cartridges. The standard
check valve cartridge contains a synthetic ruby ball and sapphire seat.
A second option for separations modules is the PerformancePLUS Check
Valve Cartridge*. (Standard for 500 & 600 Series pumps.) T his cartridge
has a larger ball and seat (3.175 mm) and internal volume. T he ball and
seat are manufactured from ruby and sapphire. T his cartridge has been
effective in solving sticking problems and provides excellent sealing
c haracteristics. T he larger orifices in this cartridge can provide an
advantage at higher flow rates.
Another optional check valve cartridge is available with a ceramic ball and
seat which can be helpful in applications where the ruby/sapphire ball and
seat-based check valves still exhibit problems with sticking.
Check valves should perform within specification for a year or more for
most customers. T his can be greatly affected by laboratory practices,
solvent preparation, c hoice of plunger seal material, and the mobile
phase required for the application. Check valve sticking can be reduced or
eliminated by experimenting with different ball and seat sizes and materials to see which provides optimal performance for the particular application
and operating environment.
* Requires PerformancePLUS Separations Module Check Valve Housing,

part number 700002332 (2/pkg).
www.waters.com

Common Parts for
Alliance Systems
HPLC Pumps
Common Parts for HPLC Pumps
Common Parts for HPLC Pumps
2690/D
2695/D
2790
2795
2796
WAT271067
WAT270959
700001326
WAT270938
WAT271066
WAT270939
WAT270940
Common Parts for Alliance Systems

Plunger Oriented (Optional)
Assembly, Plunger (Standard)
Head, Plunger Seal kit (clear)
Head, Plunger Seals Repl. Kit
(Std-Yellow)
Head, GFP Plunger Seal Kit
(Optional Black)
Head, Face Seals
Replacement Kit (4)
Wash Tubes Seals
Replacement Kit, S/W
Seal Wash Face Seal
Replacement Kit
Seal Wash Plunger Seal,
Replacement Kit
Check Valve Cartridge
Replacement Kit (2 cart.)
PerformancePLUS Check
Valve cartridge
Check Valve cartridge
Ceramic Check Valve
Valve Housing
Inline Filter
Filter Insert
Assembly, GPV
Nut, Head
Degasser Chamber
25 L Syringe
250 L Syringe
500 L Syringe
1000 L Syringe
2500 L Syringe
PerformancePLUS Needle
HPMV Rebuild Kit
HPMV & Seal Tool Kit
Assembly, Seal Pack
Replacement Kit w/Needle
Seal Pack Rebuild Kit w/Needle
Kit, Carousel Set, 5/pk
Inject Port
Seat, Inject Port
Seal, Inject Port Washer
2790 Needle
Syringe, 100 L
www.waters.com
WAT271017
WAT271018
WAT270941
700002399
700002761
700002333
700002332
WAT035190
WAT088084
WAT270927
WAT270964
700001218
WAT077343
WAT073109
700000565
700000611
WAT077342
700001247
WAT045424
WAT045427
700002791
WAT271019
WAT270328
700000383
700000384
700000385
700000389
700000564
1525 micro Seal Kit

Active seal wash kit 1525
Active seal wash kit 1525 EF
Active seal wash kit 1525 micro
AQ Seal repl. 2pk
AQ Seal repl. 4pk
Plunger & wash seal kit (2) 626
Plunger Seal Black 1/pk
Plunger Seal UP30 1/pk
Plunger Seal, TAN (Rulon)
Pump Seal Kit w/Spring
Seal Clear-100
Seal Plunger (Ext. Flow)
Seal, Clear-100 4/pk
Seal, Kit black, replace
Seals Aqueous, Buffer, 2/pk
Seals, Aqueous, Buffer, 4/pk
1525, Check Valve, 2/pk
B & S Check Valve Kit
Extended Flow Update Kit
Housing Outlet
Inlet Check Valve
Inlet Check Valve Assembly
Inlet Check Valve Housing
Inlet Housing
Inlet Rebuild Kit
Outlet Check Valve
Outlet Check Valve
Outlet Check Valve Housing
Outlet Check Valve Housing
Outlet Check Valve Rebuild Kit
PerformancePLUS Cartridge
w/Housing (2)
PerformancePLUS Ceramic
Valve Cartridge
Plunger Wash Kit (225 L)
Assembly, Plunger
Assembly, Plunger Sapphire 1/8
Oriented Plunger, 510
Plunger
Plunger w/Indicator Rod
Retaining Ring
Assembly, Solvent Filter
Assembly Difusser
Reference Valve 600/510
Kit Ref. Vlv Rebld Pmp
Syringe, 10 mL Safety
Head Support Bushing
Ind. Rod Kit
515
205000202
205000251
205000252
205000250
WAT025296
WAT025297
WAT031790
WAT026644
700002282
WAT025384
WAT069581
WAT022934
WAT026644
WAT022946
WAT026613
WAT025296
WAT025297
700002275
WAT088223
WAT024120
WAT094003
WAT025212
WAT060307
WAT033679
WAT030541
WAT060308
WAT025203
WAT060495
WAT025028
WAT025216
WAT025207
WAT030543
WAT026014
600
1515
1525
1525
micro
Ext
Flow
700000253
700002399
700000254
WAT030852
WAS207069
WAT025656
WAT069511
WAT060304
WAT031788
WAT025360
WAT025531
WAT007272
WAT026592
WAT025746
WAT027629
WAT060305
WAT069583
626
295

HPLC Pumps
515 HPLC Pump
Description
Part No.
515 HPLC Pump Performance Maintenance Kit
WAT052587

Parts & Accessories
PerformancePLUS cartridge check valves with housing
Clear-100( plunger seals (4/pk)
Plunger insertion tool
Snap ring pliers
Aqueous seal kit, 100 L (4/pk)
Priming syringe needle
Safety syringe, 10 mL
PerformancePLUS check valve cartridges (2/pk)
700000253
WAT022946
WAT011042
WAT025263
WAT025297
WAT025559
WAT027629
700000254
Clear-100 Plunger Seals, 4/pk

WAT022946
515 Performance Maintenance Kit
1515 Series HPLC Pump
Description
Part No.
1515 Series HPLC Pumps Performance Maintenance Kit
201000113

Parts & Accessories
PerformancePLUS check valve cartridges
Clear-100 plunger seals (4/pk)
Solvent filter

WAT022946
700000254
WAT022946
WAT025531
Solvent Filter
WAT025531
296
www.waters.com
1525 Series HPLC Pump
Description
Part No.
1525 Series HPLC Pumps Performance Maintenance Kit
201000114

Parts & Accessories
PerformancePLUS check valve cartridges
Solvent filter
1525 to 1525 EF Conversion Kit

WAT022946
700000254
WAT022946
WAT025531
205000324
Solvent Filter
WAT025531
1525 micro HPLC Pump
Description
Part No.
1525 micro HPLC Pump Performance Maintenance Kit
201000161
1525 micro Performance

Maintenance Kit
Waters Critical Clean: One of the advantages of using Waters Parts

Waters Critical Clean components and assemblies that come into direct contact with your sample analysis are treated with
additional care so that they are free from organic, inorganic, and particulate contaminants. T his special care includes:
Stringent cleaning processes after manufacture
Finished components handled within clean room environments
Packaging in protective materials
www.waters.com
297

Gradient Modules
2545/2525 Binary Gradient Module
Description
Part No.
2545/2525 Binary Gradient Module Performance Maintenance Kit
201000130

Parts & Accessories
Check valve cartridge kit (4/pk)
Plunger seal kit (4/pk)
700001493
700001494
2535 Quaternary Gradient Module
Description
Part No.
2535 QGM Performance Maintenance Kit
201000209

Parts & Accessories
Assembly, Leak Sensor
Plunger C-Clip
Tubing, Mixer to Vent Valve
Assembly Tube, Right Transducer Outlet
Housing, Inlet Check Valve
Block, Stop valve positioning
Drip Tray, Leak Sensor
Inlet Manifold Block
Bracket Mixer Inlet, Manifold Z
Manifold, Outlet Check Valve, 25X5
Pump Head Assembly
Pump Head Support Plate
Solvent Filters, 4/pk
Helium Connection Kit
Assembly, Solvent Filters (Sparge and Solvent)
205000505
410000570
430002032
430002121
700001529
700001530
700004425
700004430
700004431
700004439
700004445
700004454
700004613
700005083
WAT023486
WAT025531
Literature
References
Waters Global Services: W hy Choose Waters Services Brochure
298
www.waters.com
Description
part no.
2545Q Performance Maintenance Kit
201000199

Parts & Accessories
Leak sensor
Plunger c-clip
Tubing, mixer to vent valve
Assembly, tube, right transducer outlet
Inlet check valve housing
Assembly, cartridge housing outlet
Block, stop, valve positioning
Drip tray, 2545Q, ls ready
Manifold, inlet
Assembly, mixer, 2545Q
Manifold, outlet ck valve, 2545Q
Pump head, .280 Id cv back
Assembly, tubing solvent inlet
Pump head support plate
Helium connection kit
Solvent Reservoir Filter
2545Q Performance Maintenance Kit
205000505
410000570
430002032
430002121
700001529
700001530
700004425
700004430
700004431
700004436
700004445
700004454
700004607
700004613
WAT023486
WAT025531
Description
Part No.
2555 QGM Performance Maintenance Kit
201000210

Parts & Accessories
www.waters.com
Assembly, Leak Sensor

Plate, Head Support
Pump Head .395
Tubing, Mixer to Vent Valve
Assembly Tube, Right Transducer Outlet
Assembly, Cartridge Housing Outlet
Outlet Pump Filter Assembly
Block, Stop, Valve Positioning
Drip Tray, LS Ready, 25 x 5
Inlet Manifold Block
Bracket, Mixer Inlet, Manifold Z
Manifold, Outlet Check Valve, 25 x 5
Assembly, Tube 1/4 Solvent Inlet (from Solvent Bottle)
Assembly Mixer
Helium Connection Kit
Assembly, Solvent Filter
Manifold, Tee 2555Q
205000505
405006414
405008440
430002032
430002121
700001529
700001530
700001836
700004425
700004430
700004431
700004439
700004445
700004607
700005084
WAT023486
WAT025531
WAT070122
299

600E Fluid Handling Unit
Description
Part No.
600E Fluid Handling Unit Performance Maintenance Kit
WAT052675

Parts & Accessories
PerformancePLUS cartridge check valves with housing
Fuse, 4A, 125V (5/pk)
Fuse, 0.5A (5/pk)
Fuse, 1.5A, 250V (5/pk)
Fuse, 1.25A, 250V (5/pk)
Fuse, 0.8A Slo-Blo (5/pk)
Fuse, 0.25A Slo-Blo for wash pump (5/pk)
700000253
WAT022946
WAT055631
WAT022628
WAT055632
WAT055633
WAT055629
WAT042200

WAT022946
600E Performance Maintenance Kit
626 LC Fluid Handling Unit
Description
Part No.
626 LC Fluid Handling Unit Performance Maintenance Kit
WAT052673

Parts & Accessories
Safety syringe, 10 mL
Fuse, 4A, 125V (5/pk)
Fuse, 5A
Fuse, 0.5A
Fuse, 1.5A, 250V (5/pk)
Fuse, 1.25A, 250V (5/pk)
Fuse, 0.8A Slo-Blo (5/pk)
Fuse, 0.25A Slo-Blo for wash pump
WAT027629
WAT055631
WAT163-18
WAT022628
WAT055632
WAT055633
WAT055629
WAT042200
626 LC Performance Maintenance Kit
300
www.waters.com
PrepLC 2000 Pump
Description
Part No.
PrepLC 2000 Pump Performance Maintenance Kit
WAT052671

Parts & Accessories
Fuse, 4A Slo-Blo
Fuse, 1A, 250V
Fuse, 3A, 250V
Fuse, 0.5A
Plunger seal
Outlet check valve
Inlet check valve
Fuse, 4A
Fuse, 2.5A
PrepLC 2000 Performance Maintenance Kit
WAS037497
WAT022615
WAT022626
WAT022628
WAT052119
WAT052220
WAT052230
WAT071406
WAT072919
Reagent Manager
Description
Part No.
Reagent Manager Performance Maintenance Kit
201000102

Parts & Accessories
EZ grip nut (3 pack)
EZ grip ferrule (3 pack)
700000146
700000145
Waters Quality Parts and Performance Maintenance Kits

Waters Quality Parts and Performance Maintenance Kits are designed and
manufactured under stringent Waters Critical Clean processes and the
same strict regulatory standards (ISO 9001:2000 and cGMP) as your highperformance Waters systems to ensure:
Continued system compliance
Maximum lifetime and reliability of both the spare part

and your instrument
Contamination free environment
Accurate and reproducible results
Use of Waters Performance Maintenance Kits offer a single, convenient

source for all the Waters Quality Parts you need to maintain your instrument
at peak performance over a 12-24 month period.
Order Waters Quality Parts and Performance Maintenance Kits online using
our easy-to-use spare parts catalog or interactive graphical interface. Go to
www.waters.com/parts.
www.waters.com
For more information, contact your local Waters representative.

In the U.S. and Canada, call 1.800.252.4742
301

Injectors/Fraction Collectors
717plus Autosampler
Description
Part No.
717plus Autosampler Performance Maintenance Kit
WAT052669
PM Kit includes: Syringe (250 L), Needle and Filter.

Parts & Accessories
HP valve rebuild kit
Syringe, 25 L
Syringe, 250 L
Syringe, 2500 L
48-vial carousel
96-vial carousel
WAT045424
WAT077343
WAT073109
WAT077342
WAT078723
WAT078727
2707 Autosampler
Description
Part No.
Parts & Accessories

Stainless steel sample loop, 20 L
Bio-compatible sample loop, 100 L
Wash bottle, glass, 250 mL
Air needles, 50 mm, yellow
Air needles, 56 mm, red
Air needles, 68 mm, blue
Air needles, 74 mm, green
Air needles, 80 mm, black
Preparative sample loop
700000680
700000683
700000684
700000685
700004063
700003872
700003921
700003922
700003923
700003924
700003925
700003928
700004086
700004088
700004089
700004090
Description
Part No.
201000196
PM Kit includes: Syringe (500 L), Needle and Rotor Seal.
302
www.waters.com
2747 Autosampler
Description
Part No.
2747 Autosampler Performance Maintenance Kit
201000132
PM Kit includes: Syringe (1.0 L), Probe, and Lubrication Kit.
2757 Sample Manager/Fraction Collector
Description
Part No.
96-well Microtiter Plate Holder Kit

4 mL Vial Holder Plate Kit
2 mL Vial Holder Plate Kit
13 mm Tube Holder Kit
Open Access 2 mL Vial Holder Kit
Open Access 4 mL Vial Holder Kit
Fraction Collector 18 mm Rack Kit
Deepwell Plate fraction collector rack kit
Flex ribbon cable
205000105
205000106
205000107
205000108
205000109
205000112
205000115
205000116
205000117
205000118
205000119
205000134
700001397
Increase Waters LC and MS

System performance with
cost-effective upgrades
Expand the analytical capabilities of your current LC,
UPLC, or MS system with a cost-effective upgrade.
For more information about Waters wide range of
instrument field upgrades and accessories, go to,
www.waters.com/upgrades
www.waters.com
303
2767 Sample Manager
Description
Part No.
2767 Autosampler Performance Maintenance Kit
201000195
PM Kit includes: Probe, Injector Port, and Rotors.

Syringes to Complement the 2767 Autosampler Performance Maintenance Kit
10 mL reagent syringe
500 mL Reagent Syringe
1.0 mL Reagent Syringe
700000471
WAT272080
WAT272617
WAT272620
WAT272623
Waters Fraction Collector III
Description
Tabletop rack (for use with prep funnel rack)
Prep funnel (32 position, 2 each set)
Prep Funnel Rack (holds up to 4 prep funnels)
4-Microtiter Plate Rack
Multi-purpose rack
Carousel Rack, (2 x 2690 Carousels)
Eppendorf Tube Collection Rack
17mm OD Vial Collection Rack
28mm OD Vial Collection Rack
Standard Test Tube Rack, 120 positions
Prep funnel, 30 Position
Tygon Tubing, 6.35 mm ID x 9.52 mm OD x 5 m,
2/pkg (use with prep funnel rack)
Test Tube Rack
Teflon Tubing 8 mm ID x 50 ft (for use with prep funnel rack)
304
Part No.
289000440
725000106
725000107
725000110
725000113
725000144
725000145
725000146
725000147
725000152
WAT037046
WAT037047
WAT037072
WAT037090
www.waters.com
Manual Injectors
Description
Part No.
3725 High Pressure Manual Valve Performance Maintenance Kit

7010 Analytical Injector Performance Maintenance Kit
7125 Sample Injector Performance Maintenance Kit
7725 Analytical Injector Performance Maintenance Kit
8125 Micro-Scale Injector Performance Maintenance Kit
9125 PEEK Valve Performance Maintenance Kit
7750E Stainless Steel Switching Platform Performance Maintenance Kit
7750E-075 Motorized Sample Injector Performance Maintenance Kit
7725 Analytical Injector Performance

Maintenance Kit
201000119
201000116
201000117
201000118
201000119
201000120
201000121
201000122
201000125
2777/2777C Sample Manager
Description
Part No.
2777/2777C Sample Manager Performance Maintenance Kit
201000162
PM Kit includes: Tension Cords and Lubrication Kit.
2777
Rotors list associated with the Valco Valves:

Rotor 10 port 2 pos 0.4 mm Cheminert Valve
Rotor 6 port 2 pos 0.4 mm LC Inj Valve
Rotor 6 port 2 pos 0.4 mm ChemInert
Rotor 10 port 2 pos 0.4 mm LC Inj Valve
Rotor 4 port 2 pos 0.5 L internal loop LC Inj Valve
700001230
700002210
700002292
700002293
700002297
700002298
700002439
Note: These rotors are not included in the PM Kit; must be ordered separately based
on the type of valve.
2777C
Literature
References
Waters Educational
Services Course Catalog,
www.waters.com
Waters Total Assurance

Warranty Solution Sheet,
305

Detectors
2487/2488 Dual-Wavelength Absorbance Detector
Description
Part No.
2487/2488 Absorbance Detector Performance Maintenance Kit

(includes PerformancePLUS deuterium lamp)
WAS081142
Parts & Accessories

Fuse, lamp power supply, 2A micro (F1 & F2 on CPU)
Flow cell rebuild kit, 10 mm
2487 Microbore Taper slit flow cell
2487 High pressure Taper slit flow cell
2487 Semi-prep Taper slit flow cell
2487 Inert Taper slit flow cell
2487 Autopurification flow cell
2487 Variable Pathlength flow cell
2488 Standard flow cell
2488 Microbore flow cell
2488 Multi UV Prep flow cell
2488 Multi UV Prep/Analytical flow cell
WAS290423
WAS081346
WAT081159
WAT081321
WAT081158
WAT081157
289000614
700000923
700001786
205000321
205000322
205000323
2489 UV/Visible Dual-Wavelength Absorbance Detector
Description
Part No.

(Includes PerformancePLUS High Brightness Lamp)
201000186
Parts & Accessories
306
Autopure flowcell
Dual path flowcell
I/O connector, 10 pin
Assembly, cable, ethernet, 10 ft straight through
Ethernet 10 ft crossover cable
MS flow cell rebuild kit
MS gasket kit, 10/pk
Analytical flowcell
Cuvette holder assembly
10 mm flowcell rebuild kit
3 mm flowcell rebuild kit
Gasket kit, 10/pk
10 mm cell, linearity solutions
Wavelength accuracy solutions
PQ test mix for absorbance detector
Two cuvette kit (empty)
Fuse, 3.25A, 250V 5 x 20 mm, fast-acting (5/pk)
Analog out cable assembly
Inert taper slit flow cell
Semi prep flowcell
Microbore flowcell
High pressure flowcell
Power Cord, 110V
289000614
289000935
323000247
441000372
700003423
700000168
700000169
WAS081140
WAS081333
WAS081346
WAS081347
WAS081348
WAT042881
WAT042885
WAT042887
700004155
700001800
WAT057235
WAT081157
WAT081158
WAT081159
WAT081321
WAT097194
www.waters.com
2996 Photodiode Array Detector (PDA)
Description
Part No.
2996 Photodiode Array Detector Performance Maintenance Kit

(includes PerformancePLUS deuterium lamp)
WAT052586
Parts & Accessories

Fuse, 4A
Analytical high pressure cell assembly, 10 mm
Analytical cell assembly, 10 mm
Inert titanium cell assembly, 10 mm
Microbore cell assembly, 3 mm
Semi-prep cell assembly, 3 mm
100 m aperture (slit) assembly
WAT057337
WAT057460
WAT057919
WAT057461
WAT057462
WAT057463
WAT057921
WAT057922
WAT057920
2996 Performance
Maintenance Kit
2998 Photodiode Array Detector (PDA)
Description
Part No.

(Includes PerformancePLUS High Brightness Lamp)
201000186
Parts & Accessories

2998 Analytical flow cell kit
2998 microbore flow cell kit
2998 semi-preparative flow cell kit
2998 autopurification flow cell kit
Fuse, 3.15A, 250V, 5 x 20 mm fast acting (2/pk)
2998 PM kit
Connector plug, 10-position
Connector shell cover
Ethernet patch cord, shielded, 10 ft.
Event cable, 6 ft.
Crossover cable, 5E, 10ft., ethernet
Tubing, 3/8 in. OD x 1/4 in. ID, tygon
Cable, assembly, shield, analog output signal cable
PEEK comression fitting
Knob, compression fitting
Convoluted tubing
205000399
205000400
205000401
205000402
700001800
201000186
323000247
323000446
441000372
441000373
700003423
700001796
WAT057235
WAT021815
WAT021816
430001556
2998 Performance
Maintenance Kit
www.waters.com
307
T he Only Long-lasting, High Energy Lamp

Designed Specifically for Waters Detectors
Now Available at Significant Savings!
Precision alignment for
maximum performance
Optimized electronic
features to ensure
reliable ignition and
longer lifetime
Independent certification
for detector safety

Dont put the integrity of your system and data at risk by using alternative lamps. Invest in a
Waters detector lamp to achieve greater performance and higher confidence in your results.
Significant discounts on selected Waters detector lamps
Save 20% when you buy one kit

25% when you buy two kits
30% when you buy three kits
Waters Detector Lamps Performance Maintenance Kits
Part No.
2487 TUV Detector Performance Maintenance Kit
WAS081142
2996 PDA Detector Performance Maintenance Kit
WAT052586
2475 Fluorescence Detector Performance Maintenance Kit
201000131
ACQUITY eLambda/PDA/TUV 2489/2998

201000186
ACQUITY UPLC ELS Detector Performance Maintenance Kit
201000159
To order, contact your local Waters Representative.

In the US/Canada, call 1-800-252-4752.
For online orders, visit www.waters.com/shop
Be sure to mention this promotion code:
Phone/Fax orders: ZP46 Online orders: lamp
To learn what makes Waters lamps superior, go to www.waters.com/lamps
T his offer cannot be combined with any other discounts or promotions. Not available to distributors or companies that resell spare parts (3rd party vendors).
Special promotion available only for the products listed above. Limited time offer.
2414 Refractive Index Detector
Description
Part No.
Pressure Relief Valve

Sample Inlet Tubing Assembly
Tubing cutter for 1/16 in. Stainless steel tubing
Tubing Cutter, Spare Blades, 3/pk
Compression Screws and Ferrules Kit, 5 each
Stainless Steel Tubing, 0.062in. x .040 in. ID x 10 ft.
Stainless Steel Tubing, 0.062in. x .009 in. ID x 10 ft.
Analog Signal Cable
IEEE-488 Cable 6ft (2 meter)
Power Cord, 110V
700001183
700001710
WAT022384
WAT022385
WAT025604
WAT026805
WAT026973
WAT057235
WAT087141
WAT097194
WAT270868
2424/2420 Evaporative Light Scattering (ELS) Detector
Description
Part No.
2424/ 2420 ELS Detector Performance Maintenance Kit

(includes PerformancePLUS lamp cartridge assembly)
201000159
Parts & Accessories

Drip Tray
Vapor Trap (10 mm OD Bottle Trap)
415000415
700000574
2465 Electrochemical Detector

Description
Description
Flowcell Kit: 0.7 mm GC Working Electrode, Salt Bridge Reference Electrode
Flowcell Kit: 2 mm GC Working Electrode, Salt Bridge Reference Electrode
Flowcell Kit: 2 mm GC Working Electrode, ISAAC Reference Electrode
Flowcell Kit: 3 mm Pt Working Electrode, ISAAC Reference Electrode
Flowcell Kit: 3 mm AU Working Electrode, HyREF Reference Electrode
Flowcell Kit: 3 mm Ag Working Electrode, HyREF Reference Electrode
Fuse, 5 x 20, 2.5A, T 250V
RS-232 Cable
Dummy FlowCell
External I/O Cable
www.waters.com
Part No.
205004100
205004115
205004215
205004220
205004325
205004330
700001004
700001942
700001943
700001948
Part No.
ISAAC Solution, 10 mL (hazardous material)

Fingertight Flowcell Fitting
Spacer, 120 m
Spacer, 25 m
Spacer, 50 m
Polishing Disk, Work Electrode
Diamond Slurry, 1 m, 10 mL
Swivel, 2465 Salt Bridge Reference Electrode
Body, 2465 Salt Bridge Reference Electrode
Salt Bridge Ag/AgCl Reference Electrode
KCl Solution for Salt Bridge Ref (50 mL)
Working Electrode Block, 2 mm GC
Working Electrode Block, 3 mm, Platinum
Working Electrode Block, 3 mm, Gold
Woking Electrode Block, 2 mm, Silver
Salt Bridge Inlet Block
Hy Ref Inlet Block
Cell Cable
Hex Key for Flow Cell Assembly
2465 Integrator Cable
2465 ISAAC Inlet Block
Polishing Disk, Reference Electrode
Capillary Conn Kit, for Micro Cell
700001949
700001950
700001951
700001952
700001953
700001954
700001955
700001956
700001957
700001958
700001959
700001960
700001961
700001962
700001963
700001964
700001965
700001968
700001985
700001994
700002003
700002069
700002103
309
2475 Multi-Wavelength Fluorescence Detector
Description
Part No.
2475 Multi-Wavelength Fluorescence Detector Performance Maintenance Kit

(includes xenon lamp assembly)
201000131
Parts & Accessories

Fuse, 4A SMD with holder (2/pk)
Flow cell
700001840
700001618
432 Conductivity Detector
Description
Part No.
Compression Screws and Ferrules, 5/pk

432 Flow Cell
Power Cord, 110V
Union
Power Cord, 220V
WAT025604
WAT043069
WAT097194
WAT097332
WAT097410
Other Systems
Alliance GPC/V 2000 System
Description
Part No.
Alliance GPC/V 2000 System Performance Maintenance Kit
201000111
PM Kit includes: Plungers, Check valves, Seals, Filters, Needle, and Syringe (5 mL).
Parts & Accessories
Vial holder assembly, 10 mL (6/pk)
Filter vial assembly (6/pk)
Filter vial plunger assembly (6/pk)
GPC 2000 carousel handle assembly
GPC 2000 20 mm cap with septa (100/pk)
Alliance GPC/V 2000 Performance

Maintenance Kit
310
600000227
600000228
600000229
700000850
600000138
Carousel 24 position
700002406
www.waters.com

Mass Spectrometers
Quadrupole Time-of-Flight (Q-Tof) Mass Spectrometers
Description
Part No.
Q-Tof Performance Maintenance Kit (for Q-Tof II, Q-Tof API US)
Q-Tof Ultima API Performance Maintenance Kit
Q-Tof Micro Performance Maintenance Kit
Q-Tof Premier Performance Maintenance Kit
Q-Tof Premier MALDI Performance Maintenance Kit
201000219
201000224
201000219
201000187
201000187
PM Kit Contains:
Source components
ESI probe components
Vacuum pump components
SYNAPT MS/HDMS Mass Spectrometers

Description
Part No.
SYNAPT MS/HDMS Performance Maintenance Kit
201000187
PM Kit Contains:
Source components
SYNAPT G2 Mass Spectrometers

Description
Part No.
SYNAPT G2 LS Performance Maintenance Kit

SYNAPT G2 NLS Performance Maintenance Kit
201000229
201000230
PM Kit Contains:
Source components
www.waters.com
311
Xevo QTof Mass Spectrometers

Description
Part No.
Xevo QTof LS (Rotary) Performance Maintenance Kit

Xevo QTof LS (Scroll) Performance Maintenance Kit
Xevo QTof NLS (Rotary) Performance Maintenance Kit
Xevo QTof NLS (Scroll) Performance Maintenance Kit
201000203
201000204
201000205
201000206
PM Kit Contains:
Source components
Xevo G2 QTof Mass Spectrometers

Description
Part No.
Xevo G2 QTof LS (Rotary) Performance Maintenance Kit

Xevo G2 QTof LS (Scroll) Performance Maintenance Kit
Xevo G2 QTof NLS (Rotary) Performance Maintenance Kit
Xevo G2 QTof NLS (Scroll) Performance Maintenance Kit
201000238
201000239
201000240
201000241
PM Kit Contains:
Source components
Waters Critical Clean: One of the advantages of using Waters Parts

Waters Critical Clean components and assemblies that come into direct contact with your sample analysis are treated with
additional care so that they are free from organic, inorganic, and particulate contaminants. T his special care includes:
312
Stringent cleaning processes after manufacture
Finished components handled within clean room environments
Packaging in protective materials
www.waters.com

Tandem Quadrupole Mass Spectrometers
Quattro Ultima Mass Spectrometer
Description
Part No.
Quattro Ultima Mass Spectrometer Performance Maintenance Kit
176002055
PM Kit Contains:
Source components
Related Parts and Accessories not included in PM Kit:
APCI probe parts kit
IonSABRE spare parts kit
MUX 4/5 PM Kit
700000338
M956335DC2
201000149
Quattro Ultima Pt Mass Spectrometer

Description
Part No.
Quattro Ultima Pt
Mass Spectrometer Performance Maintenance Kit
176002055
PM Kit Contains:
Source components
MUX 4/5 Kit
700000338
M956335DC2
201000149
Quattro micro Mass Spectrometer

Description
Part No.
Quattro micro Mass Spectrometer Performance Maintenance Kit
176002050
PM Kit Contains:
Source components
MUX 4/5 parts kit
201000149
Quattro micro GC Mass Spectrometer

Description
Part No.
Quattro micro GC Mass Spectrometer Performance Maintenance Kit
201000170
PM Kit Contains:
Source components
www.waters.com
313
Quattro Premier / Premier XE Mass Spectrometer

Description
Part No.
Quattro Premier (Rotary) Mass Spectrometer Performance Maintenance Kit

Quattro Premier (Scroll) Mass Spectrometer Performance Maintenance Kit
176002053
176002054
PM Kit Contains:
Source components
Related Parts and Accessories:
MUX 4/5 parts kit
M956335DC2
201000149
700000338
TQ Detector
Description
Part No.
TQD (Rotary)Performance Maintenance Kit with Chemistry

TQD (Oil Free)Performance Maintenance Kit with Chemistry
176002064
176002135
PM Kit Contains:
Source components
Atmospheric Solids Analysis Probe (ASAP)

Extend sample analysis capabilities and instrument utilization of
your Waters SQD/3100 and TQD instruments by upgrading with the
Atmospheric Solids Analysis Probe.
With the Atmospheric Solids Analysis Probe (ASAP) you can now perform
rapid direct analyses of volatile and semi volatile solid and liquid samples
with SQD/3100/TQD instruments, thereby improving your laboratorys
flexibility and analytical capabilities.
ASA P enables new analyses to be carried out on your existing
instrumentation, providing increased asset utilization and optimum
productivity for only a small capital outlay.
Description
High sensitivity analysis of low polarity or non-polar

compounds unable to be ionized by ESI, APcI or APPI
Direct analysis of complex mixtures no need for sample

preparation or chromatographic separation
Good alternative to EI/CI solids probe analyses, without

the need for a vacuum lock
Part No.
SQD/TQD/3100 - ASAP Accessory
314
T he ASAP technique offers:
176002049
www.waters.com
Xevo TQ
Description
Part No.
Xevo TQ (Rotary) Performance Maintenance Kit with Chemistry

Xevo TQ (Scroll) Performance Maintenance Kit with Chemistry
176002058
176002059
Related Parts
APCI Probe Service Kit
Outer Appi Source Service Kit
700004673
700004730
Xevo TQ-S
Description
Part No.
Xevo TQ-S (Rotary) Performance Maintenance Kit with Chemistry

Xevo TQ-S (Scroll) Performance Maintenance Kit with Chemistry
201000236
201000235
PM Kit Contains:
Source components
Increase Waters LC and MS

System performance with
cost-effective upgrades
Expand the analytical capabilities of your current LC,
UPLC, or MS system with a cost-effective upgrade.
For more information about Waters wide range of
instrument field upgrades and accessories, go to,
www.waters.com/upgrades
www.waters.com
315
Single Quadrupole Mass Spectrometers

EMD 1000/ZQ Mass Spectrometer
Description
Part No.
EMD 1000/ZQ Mass Spectrometer Performance Maintenance Kit
176002050
PM Kit Includes:
Source components
APCI probe spare parts kit
700000338
EMD 1000
ZQ Mass Spectrometer
3100 Mass Detector

Description
Part No.
3100 (Rotary) Performance Maintenance Kit with Chemistry

3100 (Oil-free) Performance Maintenance Kit with Chemistry
176002064
176002135
PM Kit Includes:
Source components
3100 Mass Detector

SQ Mass Detector
Description
Part No.
SQD (Rotary) Performance Maintenance Kit with Chemistry

SQD (Oil-free) Performance Maintenance Kit with Chemistry
176002064
176002135
PM Kit Includes:
Source components
SQD/TQD/3100 - ASAP Accessory
176002049
SQ Mass Detector
316
www.waters.com
Time-of-Flight (TOF) Mass Spectrometers

MALDI micro MX Mass Spectrometer
Description
Part No.
MALDI micro MX Mass Spectrometer Performance Maintenance Kit
201000178
PM Kit Includes:
Source Components
Vacuum Pump Components
LCT Mass Spectrometer

Description
Part No.
LCT Performance Maintenance Kit with Chemistry

LCT MK II Performance Maintenance Kit with Chemistry
176002062
176002063
PM Kit Includes:
Source components
LockSpray source parts kit
MUX 4/5 parts kit
700000338
M956335DC2
201000144
201000149
LCT Premier/ LCT Premier XE Mass Spectrometer

Description
Part No.
LCT P/XE (Rotary) Performance Maintenance Kit with Chemistry

LCT P/XE (Scroll) Performance Maintenance Kit with Chemistry
176002060
176002061
PM Kit Includes:
Source components
Reference probe components
MUX 4/5 parts kit
700000338
M956335DC2
201000149
Literature
References
Edwards XDS Dry Vacuum Scroll Pump Conversion for
LCT Premier, LCT Premier XE Mass Spectrometers,
visit www.waters.com/upgrades
www.waters.com
317
GCT Mass Spectrometer

Description
Part No.
GCT Mass Spectrometer Performance Maintenance Kit
201000147
PM Kit Includes:
Source components
Related Parts and Accessories not included in PM Kit
Field ionization/field desorption source PM Kit for GCT
201000146
GCT Premier Mass Spectrometer

Description
Part No.
GCT Premier Mass Spectrometer Performance Maintenance Kit
201000188
PM Kit Includes:
Source components
Related Parts and Accessories not included in PM Kit
Field ionization/field desorption source PM Kit for GCT Premier
201000189
Magnetic Sector Mass Spectrometer

AutoSpec/AutoSpec Premier Mass Spectrometer
Description
Part No.
AutoSpec Premier Mass Spectrometer Performance Maintenance Kit
201000145
PM Kit Includes:
Source components
Electron impact (EI) source for Autospec
Chemical Ionization (CI) source for Autospec
Alternate CI/EI (ACE) source for Autospec
Field desorption (FD) source for Autospec
LSIMS/CS Gun source for Autospec
318
201000152
201000151
201000153
201000155
201000150
www.waters.com
[ bE aSSUREd ]
Choose Waters Global Services
Advance the Business of Science
with Empower Driven Services
Optimize laboratory resources through continuous improvement and
life cycle asset management. Empower Driven Services integrates
instrument services and the business analytics managed by Empower
Software to uncover efficiencies and cost savings. Learn more at
www.waters.com/empowerdriven.
Protect Your System Investment

Only the worldwide Waters service team has the knowledge, experience, and
technical and application support to ensure that your mission-critical system
operates with optimum productivity and lowest total cost-of-ownership.
Learn more at www.waters.com/plans.
Enhance Performance; Extend System Lifetime

Waters offers a range of instrument upgrades that give you access to the
latest tec hnologies to improve instrument control and data acquisition
functions, increase productivity, and extend system lifetime. Learn more at
www.waters.com/upgrades.
Keep Your Laboratory Skills up to Date

From instrumentation operation and maintenance, to applications and information management solutions, whether you are a new or an experienced
user, Waters has a course for you. To learn about courses or request a FREE
copy of the 2009-2010 course catalog, go to www.waters.com/training.
Increase Laboratory Productivity

Connections INSIGHT is an enabling technology that monitors the health
of your instruments and provides real-time collaboration with Waters worldwide team of technical experts for maximum system uptime. Learn more at
www.waters.com/insight.
One-Click, Online Access to

Waters Service Teams
Requesting phone support, onsite service, and training for your Waters
instruments and software has never been easier with iRequest Online
Service Request System. Learn more at www.waters.com/irequest.
For Trouble-Free System Operation,

Use Waters Quality Parts
Waters designs and manufactures Waters Quality Parts to the same strict
regulatory standards as your high-performance Waters systems to ensure
continued system compliance, maximum lifetime and reliability, and
accurate and reproducible results. Learn more at www.waters.com/parts.
Optimize Qualification Efficiency.

Ensure Full Compliance.
Using automated compliance tools as well as conventional qualification
workbooks and testing materials, trained and certified Waters Compliance
Service Specialists use proven procedures and tec hnologies to help you
integrate or maintain LC or MS systems and software into your regulated
laboratory. Learn more at www.waters.com/compliance.

General HPLC Parts
Column and Cartridge Fittings and Accessories
ACQUITY Column Replacement Parts
ACQUITY UPLC Column In-Line Filter Unit
ACQUITY UPLC Column Replacement Parts
Description
Part No.
In-line filter holder and six 0.2 m

stainless steel replacement filters
205000343
700002775
Description
Part No.
Three 0.2 m Inlet/Outlet Frits for 2.1 mm i.d. UPLC Columns

Three 0.2 m Inlet/Outlet Frits for 1.0 mm i.d. UPLC Columns
One Inlet End Nut for 2.1 mm i.d. UPLC Column
One Outlet End Nut for 2.1 mm i.d. UPLC Column
One Inlet End Nut for 1.0 mm i.d. UPLC Column
One Outlet End Nut for 1.0 mm i.d. UPLC Column
700003776
700003775
700003779
700003780
700003777
700003778
Waters Column and Cartridge Replacement Parts
Parker Style Cartridge Fittings and Accessories
End Connector Kit

(endfittings for cartridge columns)
T hese endfittings and accessories can be used with 4.6 mm, 4.0 mm, or
3.0 mm i.d. cartridges.
1
Description
Part No.
End connector kit

(contains 1 pair of endfittings, c-clips and coupling)
Replacement o-ring 2/pk
Replacement c-clip 1/pk
WAT037525
WAT023401
WAT037560
Replacement Filter Assemblies

for Columns
Column i.d.
Porosity
Part No.
1 mm
1 mm
2.1 mm
2.1 mm
3.0, 3.9, 4.6 mm
3.0, 3.9, 4.6 mm
7.8 mm
7.8 mm
19 mm
30 mm
2 m
0.5 m
2 m
0.5 m
2 m
0.5 m
2 m
5 m
2 m
2 m
600000175
600000176
600000177
600000178
600000179
600000180
600000181
600000182
600000183
600000184
320
Description
Qty.
Part No.
Removable Column Endfitting

Frit Assembly (2 m)
Frit Assembly (0.5 m)
Column Coupler
Long Tail Endfitting
Extended Endfitting for use with 10 mm Integral Guard
Nylon Column Plugs for Storage of Complete Column
Nylon Column Caps for Storage of Replacement
Cartridge Column
Inline 10 mm Guard Cartridge Holder Kit
for use with above items
2/pk
5/pk
5/pk
2/pk
2/pk
1/pk
1/pk
PSS614100
PSS614103
PSS614104
PSS614102
PSS614101
PSS614108
WAT015674
10/pk
PSS614113
PSS830008
30 mm Stand Alone Guard/Column (endfittings not included)

Extended endfitting for use with 10 mm Integral Guard: PSS614108
3 10 mm Integral Guard Column
4 Column Coupler : PSS614102
2
www.waters.com
SLIPFREE Connectors
New Generation HPLC Column Connector
SLIPFREE Fittings
Guarantees a void-free connection because it pushes the tubing

into the end-fitting; T his design must come installed on the tubing.
Fingertight to 10,000 psiNever need wrenches.
Readjusts to all column end-fittings; Compatible with all

commercially available end-fittings that we have tried.
Stainless stell tread for good stability and no particle generation.
Unique design separates tube holding function from sealing function.
Part No.
Single SLIPFREE, 6 cm long, 0.005 in. i.d.

Double SLIPFREE, 6 cm long, 0.005 in. i.d.
PSL618000
PSL618004
PSL618006
PSL618008
PSL618010
PSL618001
PSL618003
PSL618005
PSL618007
PSL618009
PSL618011
T he SLIPFREE connector comes standard with a replaceable Vespel ferrule.
Single SLIPFREE
0.010 inch i.d. is recommended for routine work.

0.005 inch i.d. is recommended for column connection to short
4.6 mm i.d. and for small-bore or microbore connections.
Double SLIPFREE
0.020 inch i.d. is recommended for prep or semi-prep connections,

or for connections ahead of the injector.
How to Use a SLIPFREE Connector

Place a SLIPFREE connector at any location in the HPLC system where
fittings must be frequently made or broke. Choose the single for installation to the injector or at any other fitting with conventional nuts and
ferrules that would seldom need to be removed. Choose the double
SLIPFREE connector for column coupling or places where both ends
must be loosened frequently.
Guard Column
or Filter
HPLC Column
Injector
Detector
Single SLIPFREE (Length as needed)
Double SLIPFREE (60 mm Length)
Single SLIPFREE (Length as needed)
A single SLIPFREE Connector has the SLIPFREE design

only on one end. T his end should be placed where
connections and disconnections are going to be made
frequently, such as the end-fitting of a column or detector. In this example, the other end-fitting is seated into
the injector using a stainless steel nut and ferrule that
is compatible with the brand of injector.
A Double SLIPFREE Connector has a SLIPFREE design

on both ends. It should be placed where both connections and disconnections are going to be made
frequently, such as between a column and guard
column. Very short (6 cm), small i.d. connectors are
available to minimize extra dead-volume. Because
SLIPFREE Connectors fit any column manufacturers
end-fitting, the two items being connected do not
have to be from the same manufacturer.
A Single SLIPFREE Connector has the SLIPFREE design

only on one end. T his end should be placed where
connections and disconnections are going to be made
frequently, suc h as the end-fitting of a column or
detector. In this example, the other end of the tubing
is seated into the detector using a stainless steel nut
and ferrule (one that is compatible with the brand of
detector. If there is not a convenient way to attach it to
the detector, a union can be used.
www.waters.com
321

PEEK Tubing & Fittings
PEEK One-Piece Fingertight Fitting, 1/16 in., 10-32 T hread
T his high performance fingertight is recommended for the most demanding applications and will resist pressures up to 6000 psi (420 Bar). T he
diameter of the head of the nuts has been increased to facilitate tightening
without toolsit is still a genuine fingertight. Nut and ferrule are made
from a single piece of PEEK.
PEEK Fingertight One-Piece Fitting
PEEK Fingertight One-Piece Fitting
0.500 in.
0.826 in.
PSL613315
Part No.
PSL613315
PEEK Two-Piece Fingertight Fittings, 1/16 in., 10-32 T hread

T hese fittings allow connections to be made by hand with a pressure rating
of 4000 psi (280 Bar). T he inexpensive PEEK ferrules will last for at
least 50 connections before it has to be replaced. T he nuts may be reused
again and again. T he PEEK ferrule is highly chemically inert and may be
used with any mobile phase. T his fitting provides a budget alternative for
HPLC plumbingwhich will fit virtually all existing HPLC fittings including Swagelok, Parker, Rheodyne, Beckman, Valco, Waters, etcall having
10-32 female threads.
0.375 in.
0.698 in.
PSL613322
0.125 in.
0.235 in.
PSL613316
PEEK Fingertight Two-Piece Fittings

PEEK Fingertight Two-Piece Nut
PEEK Single Ferrule
Part No.
PSL613322
PSL613316
PEEK Fittings with Double-Ferrules, 1/16 in., 10-32 T hread

T hese fittings use a DOUBLE-FERRULE made of PEEK which grips the tubing
in two places for unmatc hed reliability. T hese ferrules actually provide
twice the holding power of single-ferrule fittings, and are ideal for use with
PEEK and Tefzel tubing, which will often slip when used with single-ferrule
fittings. W hen used with stainless steel or titanium tubing, Double-Ferrule
Fittings grip tighter for a more reliable connection at high pressure.
0.125 in.
0.275 in.
PSL613302
1/4 in.
0.605 in.
We offer both fingertight and hex-head nuts for use with Double-Ferrules.
T he fingertight version may be hand-tightened for operation at up to
6,000 psi. Use the hex-head version for connections that are difficult to
reach or closely spaced.
T hese fittings fit virtually any female 1/16 in. fittingParker, Swagelok,
Waters, Valco, Rheodyne, Upchurch, etcall having 10-32 threads.
PSL613324
0.475 in.
0.700 in.
PSL613301
PEEK Fittings With Double-Ferrules

PEEK Double-Ferrule
PEEK Hex-Head Nut
PEEK Fingertight Nut
Stainless Steel Fingertight Nut
322
Part No.
PSL613302
PSL613324
PSL613301
PSL613325
1/2 in.
0.525 in.
PSL613325
www.waters.com
PTFE/ETFE Tubing and Fittings
Compression Screws & Ferrules
o.d. Inches (mm)
i.d. Inches (mm)
Length/Material
Part No.
0.125 (3.2)
0.149 (3.8)
0.250 (6.3)
0.080 (2.0)
0.178 (4.52)
0.149 (3.8)
0.125 (3.2)
0.125 (3.2)
0.125 (3.2)
0.062 (1.57)
0.062 (1.57)
0.062 (1.57)
0.119 (30.0)
0.190 (4.8)
0.058 (1.5)
0.148 (3.76)
0.119 (30.0)
0.020 (0.508)
0.009 (0.228)
0.040 (1.0)
0.009 (0.228)
0.040 (1.0)
25 ft. (7.6 m), PTFE

25 ft. (7.6 m), PTFE
10 ft. (3 m), PTFE
25 ft. (7.6 m), PTFE
25 ft. (7.6 m), PTFE
20 ft. (6 m), PTFE
10 ft. (3 m), PTFE
10 ft. (3 m), PTFE
10 ft. (3 m) , PTFE
36 in. (1 m), ETFE
36 in. (1 m), PTFE
WAT026808
WAT026809
WAT026810
WAT026974
WAT051041
WAT051052
WAT088430
WAT088431
WAT088432
WAT088561
WAT088563
PTFE Adapter, 0.125 (3.2) to 0.065 (1.6) 5/pk
WAT005137
Stainless Steel Tubing and Fittings
Description
Part No.
Ferrule, 01, Stainless Steel, 10/pk

Compression Screw, 0.0625 in., 10/pk
Compression Fitting Plug, Stainless Steel, 5/pk
Rheodyne Ferrule, 10/pk
Ferrule, Stainless Steel
Ferrule, 1/16 in. o.d., PEEK
Compression Screw, Stainless Steel
Compression Fitting Plug, Stainless Steel
Compression Screws & Ferrules, 0.166 in., 5/pk
Compression Screws, 0.125 in., PEEK, 2/pk
Compression Screw, Long, 1/16 in.
Compression Screw, Short, PEEK 1/16 in.
Extra Long Compression Screw, Stainless Steel, 10/pk
Finger Tight Poly Knob used with compression screws plus PEEK ferrules
Tee, 0.0625 in. Compression Screw, Stainless Steel
Tubing Cap, Hex Stainless Steel
Union, 0.0625 in. Stainless Steel
WAT005063
WAT005070
WAT005079
WAT007020
WAT022330
WAT021817
WAT025313
WAT025566
WAT025604
WAT046-12
WAT021812
WAT021815
WAT060051
WAT021816
WAT075215
WAT084078
WAT097332
PEEK Tubing and Fittings
o.d. Inches (mm)
i.d. Inches (mm)
Length/Material
Part No.
0.0625 (1.6)
0.0625 (1.6)
0.0625 (1.6)
0.0625 (1.6)
0.125 (3.2)
0.125 (3.2)
0.0625 (1.6)
0.005 (0.127)
0.020 (0.508)
0.030 (0.762)
0.040 (1.020)
0.062 (1.57)
0.093 (2.36)
0.009 (0.228)
10 ft. (3 m), SS
10 ft. (3 m), SS
10 ft. (3 m), SS
10 ft. (3 m), SS
10 ft. (3 m), SS
10 ft. (3 m), SS
10 ft. (3 m), SS
WAT241039
WAT026804
430000366
WAT026805
WAT026806
WAT026807
WAT026973
0.0625 inch o.d. Stainless Steel Tubing Cutter with 3 Blades

Replacement Blades for WAT022384, 3/pk
o.d. Inches (mm)
i.d. Inches (mm)
Length/Material
Part No.
0.0625 (1.6)
0.0625 (1.6)
0.0625 (1.6)
0.0625 (1.6)
0.005 (0.127)
0.010 (0.254)
0.015 (0.381)
0.020 (0.508)
5 ft. (1.5 m), PEEK

5 ft. (1.5 m), PEEK
5 ft. (1.5 m), PEEK
5 ft. (1.5 m), PEEK
WAT022995
WAT022996
WAT022997
WAT022998
PEEK Tubing Cutter

PEEK Tubing and Fitting Kit
PEEK Union, 0.0625 in.
WAT031795
WAT022999
WAT026-04
WAT022384
WAT022385
Filters
Last Drop Mobile Phase Filters
T he Last Drop Mobile Phase Filter utilizes a flat filter element whic h
sits parallel to the bottom of the reservoir. T his design allows the filter
to draw all but the last 2% of the mobile phase from the reservoir
without drawing air into the system. Last Drop Filters are available with
316L Stainless Steel or PAT (PEEK alloyed with Teflon) filter elements
in inert Teflon housings. T he top of the housing has a PEEK tripod which
fits into 1.5, 2.2 or 3.5 mm id pump inlet lines.
Last Drop Mobile Phase Filter

with 2 m Stainless Steel Filter
with 10 m Stainless Steel Filter
with 2 m PAT Filter
with 5 m PAT Filter
www.waters.com
Part No.
PSL901290
PSL901291
PSL901292
PSL901294
Universal PEEK
Tubing Adapter
Teflon Filter Body
Stainless Steel
or PAT
Filter Element
323
PEEK Unions, Tees, and Crosses

T he PEEK union allows you to connect two pieces of 1/16 in. tubing quickly
and reliably and can be used up to 6000 psi. Because this is a PEEK union,
it is absolutely inert and biocompatible. PEEK Tees and Crosses may be
used up to 10,000 psi.
Connects any 1/16 in. Tubing PEEK,

SS, Ti, Tefzel)
Low Dead Volume
10-32 T hread
Description
Part No.
PEEK Union with 2 PEEK Fingertight Nuts and Double Ferrules 1/16 in.
PEEK Union without Nuts and Ferrules 1/16 in.
PEEK TEE with One Piece Fingertight Fitting
PEEK CROSS with One Piece Fingertight Fitting
PEEK TEE Without Fittings
PEEK CROSS Without Fittings
PEEK One Piece Fingertight Fitting
PSL613312
PSL613313
PSL613317
PSL613319
PSL613318
PSL613320
PSL613315
PEEK union
PEEK Starter Kit

By replacing stainless steel parts, such as tubings, fittings, ferrules, mobile
phase filters, in-line filters, etc. you are creating a biocompatible and metalfree environment for your samples and mobile phase. The PEEK Starter Kit is
a collection of items which we believe is a good start for everybody working
in the field of Biochromatography. Each kit is supplied in a sturdy plastic
case. T here is an approximate saving of 25% in buying this kit compared to
the individual components.
Description
Part No.
PEEK Starter Kit
PSL613321
Contains the following :

PEEK Fingertight One-Piece (6), PEEK Handtight Nut (4), PEEK Hex-Head Nut (4), PEEK
Double Ferrules (20), PEEK Tubing 1/16 in. x 0.25 mm (1 x 3 m), PEEK Tubing 1/16
in. x 0.50 mm (1 x 3 m), PEEK Union (1), Elbow 90 Degrees (2), Elbow 180 Degrees
(2), Guillotine Cutter (1), PAT Mobile Phase Filter - Last Drop (1)
Handilok CTFE Fittings

Handilok fittings can replace conventional compression fittings used with
1/16 in. tubing without the need for tools and are compatible with all
internal fittings having a 10-32 thread. T hey meet rigid high pressure
requirements and will stand pressures in excess of 4000 psi.
Handilok Fittings
Qty.
Part No.
1/16 in. fitting

1/16 in. fitting
1/pk
10/pk
PSL618021
PSL618022
324
Handilok CTFE Fitting

Part No.: PSL618021
www.waters.com
PEEK Biocompatible Mobile Phase Filter

T he mobile phase filter protects your HPLC pumping system against small
particles in the mobile phase. Many macromolecules are fairly labile
and require not only biocompatible c hromatographs, but also mobile
phase filters which are totally inert. T hese filters are designed from inert
polymeric components, which effectively eliminate metal from the fluid
path. With a porosity of 5 m all fittings, including the inlet tube, are
manufactured in totally inert PEEK.
Description
Part No.
Biocompatible Mobile Phase Filter
PSL901282
Solvent Inlet Filters

It is good practice to always filter your solvents to prevent any pump
damage. T hese 10 m filters provide the pump protection required.
T heir large surface area means long life without pump cavitation.
Description
Filters should be changed periodically depending on usage and mobile

phase. Replacing the filter is easy; no tools are needed. T he unique
Plastictight male nut is screwed into the filter and tightened by hand.
Finger tightening is all that is necessary; the Plastictight fitting holds
without flanging.
Solvent Inlet Filter Kit - Contains one 3 in. length of Teflon Tubing,
1 Plastictight Fitting, 5 Replacement 10 m Filters
Plastictight Fitting with Teflon Tubing
1/16 in. i.d. x 1/8 in. o.d. x 3 ft.
Replacement Filter 10 m 5/pk
Part No.
Solvent Inlet Filter Kits

PSL613600
PSL613602
PSL613604
Solvent Inlet Filters for General Use

Solvent Inlet Filter 10 m with 1/16 in. o.d. stem for 1/8 in. o.d. tubing
Solvent Inlet Filter 10 m with flangeless fittings for 1/8 in. o.d. tubing
PSL613570
PSL613578
Solvent Inlet Filters for Preparative HPLC

Part No.
PSL613578
Solvent Inlet Filter 10 m with flangeless fittings for 1/8 in. o.d. tubing
Part No.
PSL613609
PSL613607
PSL613608
Solvent Inlet Filters for Waters HPLC Systems

PSL613609
Bottom-of-the-Bottle Solvent Filters

T his filter has been designed after the original Bottom-of-the-Bottle
replaceable filters. T his unique filter has a stainless steel stem on top to
accommodate 1/16 in. i.d. tubings. T he lower stem which goes directly
into the 10 m filter reac hes to within 0.06 in. of the Bottom-of-theBottle filters. T he Bottom-of-the-Bottle Solvent Filter has a 10 m filter
that can easily handle flow rates up to 10 mL/min.
Bottom-of-the-Bottle Solvent Filter

Stainless Steel Filter Assembly
www.waters.com
Stainless
Steel Stem
Part No.
PSL613457
325

Notes
326
www.waters.com
[ Indices ]
Alphabetical Index....................................................................................................................................................................................328
Part Number Index ....................................................................................................................................................................................336
327
aLPhabETICaL INdEx
Carbamate Analysis Column............................................................................171
Accell Plus ..................................................................................................................230

96-well Plates .................................................................................................... 28
Ion-echange Bulk Packings ..............................................................................230
PrepPak Cartridges ...........................................................................................230
Sep-Pak Cartridges ...................................................................................... 39, 48
Carbohydrate Amide Columns ..................................................................94, 126

Carbohydrate Analysis Column .......................................................................170
Column Selection Guide ...................................................................................109
Delta-Pak Columns............................................................................................159
Fatty Acid Analysis ..........................................................................................171
AccQTag Ultra UPLC Method for Amino Acid Analysis .........................................240
Fermentation Analysis, Organic Acids, Alcohols, and Carbohydrates ..........173
AccQTag HPLC Method for Amino Acid Analysis...................................................241
Free Fatty Acid HP Analysis Column ...............................................................171

Gas-Chromatography Packings ........................................................................176
ACQUITY UPLC
ACQUITY CSH Columns ..................................................................................... 91
Guard Products ..................................................................................................163
ACQUITY Bisphenol A Column and Method Kits .............................................. 52
Guard-Pak Holder..............................................................................................165
ACQUITY Column Listings.................................................................................. 88
Guard-Pak Inserts ..............................................................................................165
ACQUITY PFC Analysis Kit................................................................................. 52
Guide to Guard Column Selection and Use .....................................................163
ACQUITY PFC Column Kit .................................................................................. 52
High Performance Carbohydrate Cartridge Column .......................................170
ACQUITY UPLC BEH200 SEC Columns ..........................................................217
High Strength Silica (HSS) HPLC Columns......................................................138
ACQUITY UPLC BEH Columns ........................................................................... 93
HPLC Columns T heory, Technology, and Practice Book..................................117
ACQUITY UPLC BEH Glycan Columns .............................................................250
IC-Pak Anion-analysis Columns .......................................................................174
ACQUITY UPLC Column Ordering Information ................................................. 99
IC-Pak Cation-analysis Columns ......................................................................175
ACQUITY UPLC High Strength Silica (HSS) Columns ....................................... 95

ACQUITY UPLC Selectivity Choices................................................................... 95
Heat Seal for ACQUITY UPLC Systems ...........................................................105
Introduction......................................................................................................... 90
nanoACQUITY UPLC Column Ordering Information.......................................262
Plates for ACQUITY UPLC Systems .................................................................104
Screw Cap 12 x 32 mm Vials for ACQUITY UPLC Systems ...................64, 103
Settings for ACQUITY UPLC Vials ..................................................................... 65
Ion Analysis ......................................................................................................174

Ion-exclusion Columns .....................................................................................175
Lanthanide Analysis .........................................................................................175
Nova-Pak Columns ............................................................................................155
Nova-Pak Column for Triglycerides and Cholesterol Analysis .......................173
PAH Columns ....................................................................................................172
Paired-ion Chromatography (PIC Reagents) ....................................................166
UPLC Column Kits............................................................................................... 97
Pesticide Analysis ............................................................................................171
VanGuard Pre-columns ....................................................................................... 96
Physical Characteristics of HPLC and UPLC Packings.....................................116
Waters ACQUITY UPLC System Sample Manager ..........................................104

ACQUITY UPLC System PM Kits
Porapak Porous Polymer Packings for GC ........................................................176

Porasil/Porasil Columns .................................................................................160
2996 PDA Detector .........................................................................................285
Resolve Columns...............................................................................................161
ACQUITY I2V BSM PM Kit ................................................................................285
Retention Time Table for Sugars and Carbohydrates......................................169
Binary Solvent Manager ...................................................................................285
SAM Reagents...................................................................................................170
ELS Detector......................................................................................................285
Sentry Guard Columns Product List ..................................................................164
FLR Detector .....................................................................................................285
Sentry Guard Holders Product List ...................................................................165
Sample Manager...............................................................................................285
Shodex RSpak Polymer Reversed-Phase Columns ..........................................162
Spare Parts & Tubings ......................................................................................285
Shodex SC-Series Columns for Carbohydrate Analysis ..................................170
TUV Detector ....................................................................................................285

TUV/PDA/eLambda Detector............................................................................285
ACQUITY H-Class System ..........................................................................................288
ACQUITY UPLC Local Console Controller Kit ............................................................289
ACQUITY UPLC FlexCart ............................................................................................291
Amino Acid Analysis
AccQ-Tag Ultra for UPLC..................................................................................239
AccQ-Tag for HPLC ...........................................................................................241
Shodex KS-Series Columns for Carbohydrate Analysis..................................170

Silica-Pak Cartridges for Carbohydrate Analysis ............................................170
Sugar and Carbohydrate Analysis ...................................................................173
Sugar-Pak 1 Columns .......................................................................................170
SunFire Columns ...............................................................................................129
Symmetry Columns ..........................................................................................144
Symmetry300 Columns...................................................................................149
Amide Columns ...................................................................................................94, 126
SymmetryShield Columns ................................................................................147
Transition Metal Analysis.................................................................................175
Atlantis Columns ..............................................................................................132
Triglycerides and Cholesterol Analysis ...........................................................173
Atlantis NanoEase Capillary Columns.............................................................260
Typical Applications for Sugar and Carbohydrate Columns ...........................168
Atlantis NanoEase Nano Columns ...................................................................260
Ultrahydrogel Columns ....................................................................................170
Atlantis NanoEase Trapping Columns .............................................................259
Styragel Column for Cholesterol Analysis ......................................................173
Bondapak/Bondapak Columns ......................................................................158
USP L Column Listing ...................................................................................113
328
Alphabetical Index
www.waters.com
aLPhabETICaL INdEx
Waters Spherisorb Columns .............................................................................152
Carbohydrate Analysis Columns................................................................................170
Waters Spherisorb Guard Cartridges................................................................154
Cartridge Fittings and Accessories ............................................................................320
XBridge Columns ..............................................................................................122
Certified Sep-Pak Solid-Phase Extraction Cartridges .................................................. 48
XSelect Columns...............................................................................................118
Collection Plates (96 and 384-Well) .......................................................................... 69
XTerra Columns.................................................................................................140
Column Fittings and Accessories ...............................................................................320
Aqueous Calibration Standards and Kits...................................................................280
Compression Screws and Ferrules .............................................................................323
Aqueous SEC Column Selection Guide ......................................................................278

Aqueous SEC Eluent Selection Guide ........................................................................278
Atlantis
Delta-Pak
HILIC Silica ........................................................................................................136
Bulk Packings....................................................................................................192
HSS T3 ..............................................................................................................135
Columns and Packings......................................................................................159
Introduction.......................................................................................................132
nanoACQUITY Ultra Performance LC Columns ...............................................261
HPI (High Pressure Inert) Analytical Columns (PEEK) ....................................159
NanoEase Nano and Capillary Columns ..........................................................260
Preparative Columns.........................................................................................159
NanoEase Trapping Columns ............................................................................259
Prep-Pak Cartridges ..........................................................................................194
Preparative OBD Columns ................................................................................177
Radial Compression Products ...........................................................................194
T3 Bonding .......................................................................................................133
Sentry Guard Columns......................................................................................163

Detectors
432 Conductivity Detector ..............................................................................310
Bioseparations and Analyses ............................................................................200-265
2414 Refractive Index Detector ......................................................................309
Amino Acid Analysis .......................................................................................238
2424/2420 ELS Detector ...............................................................................309
Glycan Separation Technology ........................................................................250
2465 Electrochemical Detector ......................................................................309
LC and MS Standards........................................................................................255
2475 Multi-Wavelength
Fluorescence Detector ......................................................................................310
MS and LC/MS Consumables for Biomolecules ...............................................253

Oligonucleotide Separation Technology..........................................................244
2487/2488 Absorbance Detector .................................................................306
Peptide Isolations and Analyses ......................................................................202
2489 Absorbance Detector ............................................................................306
Protein Isolations and Analyses ......................................................................215

BioSuite
2996 Photodiode Array Detector ...................................................................307

Dextran Aqueous Standard Kit ...................................................................................280
Cation-exchange Columns................................................................................213
DisQuE Dispersive Sample Preparation Kits ................................................................ 54
HPLC Columns for Protein and Peptide Separations .......................................212
DNA/RNA/Oligonucleotides .............................................................................246-248
Hydrophobic-interaction Columns (HIC) ..........................................................237

Peptide Analysis Columns ...............................................................................212
Reversed-Phase Chromatography Columns.....................................................236
End Connector Kit (for Waters Style Cartridge Columns) ..........................................320
Size Exclusion Chromatography (SEC) Columns .............................................231
Envirogel High-Resolution GPC Cleanup Columns....................................................277
Bondapak/Bondapak
Environmental Solution Kits......................................................................................... 52
Analytical Columns ..........................................................................................158
EPA Method 1694 Analysis Kit................................................................................... 52
Bulk Packings....................................................................................................191
Explosives Analysis ...................................................................................................... 47
Extraction Manifolds ..................................................................................................... 38
Extraction Manifold............................................................................................. 38
Preparative Packing Characteristics.................................................................179
Extraction Manifold Accessories and Spare Parts ............................................. 38
Oasis Manifold for Extraction Cartridges .......................................................... 38
Radial Compression Columns...........................................................................194
Oasis Manifold for Extraction Plates ................................................................. 38

Bottom-of-the-Bottle Solvent Filters..........................................................................325
F
Fast Fruit Juice Analysis Column...............................................................................173
Fermentation Analysis, Organic Acids, Alcohols,
Capillary Columns
and Carbohydrates......................................................................................................174
Atlantis .............................................................................................................260
Ferrules .......................................................................................................................322
Symmetry300 ..................................................................................................260
Filter Assemblies for Columns ...................................................................................320
Symmetry .........................................................................................................260
Filtration.................................................................................................................. 80-84
XTerra ................................................................................................................260
Membrane Choices Chart.................................................................................... 82
Carbamate Analysis Kit for Food Testing .................................................................... 52
Pall Life Sciences Laboratory Sample and

Solvent Filtration Products ................................................................................. 81
Carbamate Analysis Column for Pesticides ..............................................................171
www.waters.com
Alphabetical Index
329
aLPhabETICaL INdEx
Pall Life Sciences Part Number Cross Reference ............................................... 83
Pall Life Sciences Solvent Filtration Product List ............................................... 83
Pall Life Sciences Syringe Filters Product List ................................................... 84
Sirocco Protein Precipitation Filtration Plate..................................................... 80
H
Handilok CTFE Fittings ...............................................................................................324
Heat Sealer for 96-Well Collection Plates .................................................................. 67
High Performance Carbohydrate Cartridge Column .................................................170
Fittings and Tubings....................................................................................................322
HILIC ...........................................................................................................................136
HPLC Parts and Accessories
600E Fluid Handling Unit............................................................................... 300
Compression Screws and Ferrules ...................................................................323
626 LC Fluid Handling Units .......................................................................... 300
Mobile Phase Filters..........................................................................................323
PrepLC 2000 Pump ......................................................................................... 301
PEEK Fittings .....................................................................................................322
Reagent Manager ............................................................................................ 301
PEEK Tubing ......................................................................................................323

PEEK Unions, Tees, and Crosses.......................................................................324
Food Solution Kits......................................................................................................... 52
PTFE/ETFE Tubing .............................................................................................323
Stainless Steel Tubing ......................................................................................323
Gas-Chromatography Packings ..................................................................................176

Gelman/Pall Life Sciences Filtration Products ............................................................. 81
Gel-Permeation Chromatography .....................................................................268-280
Aqueous SEC Column Selection Guide ............................................................278
Aqueous SEC Eluent Selection Guide ..............................................................278
SLIPFREE Connectors .......................................................................................321

HSPGel Columns .........................................................................................................275
HSS ACQUITY Columns................................................................................................ 95
HSS HPLC Columns ....................................................................................................138
HSS T3 Columns .........................................................................................................135
Calibration Standards and Kits ........................................................................280
Envirogel High Resolution GPC Cleanup Columns ..........................................277
IC-Pak
GPC Column Selection Guide ...........................................................................270
Anion Analysis Columns ..................................................................................174
GPC Solvent Selection Guide ...........................................................................271
Cation Analysis Columns .................................................................................175
HSPgel Columns for High Speed GPC Analysis ..............................................275
Ion Exclusion Columns......................................................................................175
Introduction.......................................................................................................268
Injectors /Fraction Collectors

717plus Autosampler......................................................................................302
Preparative GPC Columns .................................................................................277
2707 Autosampler ..........................................................................................302
Shodex GPC Columns .......................................................................................276
2747 Autosampler ..........................................................................................303
Styragel HR High-Resolution Columns............................................................272
2757 Sample Manager/Fraction Collector .....................................................303
Styragel HT High-Temperature High-Stability Columns .................................273
2767 Autosampler ..........................................................................................304
Styragel HMW High Molecular Weight Columns.............................................274
2777/2777C Sample Manager ......................................................................305
Ultrahydrogel Columns for Aqueous SEC .......................................................279
Manual Injector Rebuild Kits ............................................................................305
Ultrastyragel High Resolution Columns ..........................................................277
Waters Fraction Collector III .............................................................................304
Gen-Pak FAX Anion-Exchange Column ......................................................................248
Ion Analysis ................................................................................................................174
Global Services ...........................................................................................................319
Ion-Exclusion Columns ...............................................................................................175
Glycan Separation Technology
Ion-Exchange Chromatography (for Protein Isolation) ..............................................223
Glycan Purification and Analysis ....................................................................250

ACQUITY UPLC BEH Glycan Columns .............................................................250
MassPREP Glycoanalysis Kits ..........................................................................252
Gradient Modules
L
Lanthanide Analysis ...................................................................................................175
Last Drop Mobile Phase Filters ...................................................................................323
2535 Quarternary Gradient Module ...............................................................298
2545 Quaternary Gradient Module ................................................................299
Manifolds
2545/2525 Binary Gradient Module .............................................................298
Extraction Manifold...................................................................................... 38, 50

Extraction Manifold Accessories and Spare Parts ...................................... 38, 50
Guard Products
Waters Spherisorb Guard Columns,
Oasis Manifold for Extraction Cartridges .......................................................... 38
Fittings, and Accessories .................................................................................154
Oasis Manifold for Extraction Plates ................................................................. 38
Guard-Pak Holder..............................................................................................165
Guide to Guard Column Selection and Use .....................................................163
Sentry Guard Columns Product List ..................................................................164
Sentry Guard Holders Product List ...................................................................163
330
Alphabetical Index
MassPREP
MassPREP Consumables for MS Analysis ..............................................253-256
MassPREP Digestion Standard Mixtures..........................................................256
MassPREP Glycoanalysis Kit............................................................................252
MassPREP MS and LC/MS
Consumables for Biomolecules ........................................................................253
www.waters.com
aLPhabETICaL INdEx
MassPREP On-Line Desalting Cartridges.........................................................258
Sample Manager PM Kit ...................................................................................290
MassPREP OST Standard..................................................................................256
TUV Detector PM Kit .........................................................................................290
MassPREP Peptide Standards ..........................................................................254
TUV Detector Spares & Tubings .......................................................................291
MassPREP Phosphopeptide Enrichment Kit.....................................................263
NanoEase
MassPREP Phosphopeptide Standards ............................................................255
Nano and Capillary Columns ...........................................................................260
MassPREP Protein Digestion Standards ..........................................................255

MassPREP Protein Standard Mix ......................................................................255
Trapping Columns .............................................................................................259

Nova-Pak
Mass Spectrometers
3100 Mass Detector ........................................................................................316
Column for Triglycerides and Cholesterol Analysis .......................................173
AutoSpec/AutoSpec Premier ........................................................................... 318
EMD 1000/ZQ ................................................................................................. 316
Prep Nova-Pak HR Columns .................................................................... 157, 191
GCT ................................................................................................................... 318
GCT Premier ..................................................................................................... 318
LCT .................................................................................................................... 317

LCT Premier/Premier XE .................................................................................. 317
Magnetic Sector Mass Spectrometer ...............................................................318
Oasis
MALDI micro MX .............................................................................................. 317
2x4 Method ........................................................................................................ 24
Q-Tof II .............................................................................................................. 311
Accessories for Extraction Cartridges................................................................ 38
Q-Tof micro....................................................................................................... 311
Accessories for Extraction Plate Manifold......................................................... 38
Q-Tof Premier ................................................................................................... 311
Elution Plates.................................................................................................... 26
Q-Tof Premier MALDI ....................................................................................... 311
Elution Plate Extraction Protocols.................................................................... 29
Q-Tof Ultima API ............................................................................................. 311
Glass Cartridges ................................................................................................. 33
Quadrupole Time of Flight (Q-Tof) Mass Spectrometers .................................311
HLB General Product Information ...................................................................... 23
Quattro micro ................................................................................................... 313
Manifold for Extraction Cartridges and Plates .................................................. 38
Quattro micro GC ............................................................................................. 313
MAX General Product Information ..................................................................... 19
Quattro Premier/Premier XE............................................................................ 314
MAX Method for Extraction of Acidic Drugs ..................................................... 19
Quattro Ultima ................................................................................................. 313
MCX General Product Information ..................................................................... 18
Quattro Ultima Pt ............................................................................................ 313
MCX Method for Extraction of Basic Compounds .............................................. 18
Single Quadrupole Mass Spectrometers ..........................................................316
Method Development Kits ........................................................................... 20, 37
SQ Mass Detector .............................................................................................316
On-Line Extraction Columns for LC/MS or LC/MS/MS....................................... 32
SYNAPT G2 Mass Spectrometers ....................................................................311
Ordering Information ................................................................................... 36-38
SYNAPT MS/HDMS Mass Spectrometers ........................................................311
Product Selection Guide ............................................................................... 34-35
Tandem Quadrupole Mass Spectrometers........................................................313
Products for Automation and Liquid Handling .................................................. 31
Time of Flight (TOF) Mass Spectrometers .......................................................317
Prospekt 2/Symbiosis Cartridges....................................................................... 33
TQ Detector ...................................................................................................... 314
Sample Extraction Products Introduction........................................................... 12
Xevo G2 QTof Mass Spectrometers ................................................................. 312
Waters Positive Pressure-96 Processor ...................................................... 38, 51
Xevo QTof Mass Spectrometers ........................................................................312
WAX General Product Information ..................................................................... 22
Xevo TQ............................................................................................................ 315
WAX Generic Method for Strong Acidic Compounds ........................................ 22
Xevo TQ-S ........................................................................................................ 315
WCX General Product Information ..................................................................... 21
MassT RAK Amino Acid Analysis Solutions...............................................................243
WCX Generic Method for Strong Basic Compounds .......................................... 21
Maximum Recovery Vials .................................................................64, 70, 71, 74, 75
OBD Preparative Column Technology ........................................................................177
Method Transfer Kits..................................................................................................... 98
Oligonucleotide Isolation and Analyses ..........................................................244-249
Mobile Phase Filtration Supplies ................................................................................323
Organic Calibration Standards and Kits for GPC.......................................................280
MS and LC/MS Consumables......................................................................................229
Ostro Sample Preparation Products ............................................................................. 53
Ozone Scrubber Cartridge............................................................................................ 46
nanoACQUITY UPLC
Binary & Auxiliary Solvent Manager PM Kit ...................................................290
PAH Columns ..............................................................................................................172
High Pressure BSM/ASM Spares & Tubings .....................................................290
Paired-Ion Chromatography (PIC Reagents)...............................................................166
High Pressure SM Spares & Tubings ................................................................291
Pall Life Sciences Filtration Products ........................................................................... 81
nanoACQUITY UPLC 2D Kit .............................................................................262
Parker Style Cartridge Fittings and Accessories .......................................................320
nanoACQUITY UPLC 2D Spares & Tubing.......................................................291
PEEK
nanoACQUITY UPLC Columns .........................................................................261
www.waters.com
PEEK Fittings and Tubing ......................................................................... 322-323
Alphabetical Index
331
aLPhabETICaL INdEx
PEEK Unions, Tees, and Crosses.......................................................................324
PrepLC
PEEK Starter Kit ................................................................................................324
Assemblies and Modules..................................................................................193
PEEK Biocompatible Mobile Phase Filter .........................................................325
Accessories and Spare Parts ............................................................................195
Peptide Isolations and Analyses .......................................................................202-214
25 mm and 40 mm Column Segments
Pesticide Analysis ......................................................................................................171

PFC ACQUITY UPLC Column Kit .................................................................................. 52
and Guards (All Chemistries) ............................................................................194

Prep Nova-Pak HR
PFC ACQUITY Analysis Kit........................................................................................... 52
Physical Characteristics of HPLC Packings ................................................................116
PIC Reagents ...............................................................................................................166
PrepPak 1000 Module ...............................................................................................194
PicoTag HPLC Method for Amino Acid Analysis .....................................................242
PrepPak Cartridges .....................................................................................................194
Polyacrylic Acid Aqueous Standard Kit for GPC .......................................................280
Protein Expression System Kits ..................................................................................263
Polybutadiene Organic Standard Kit for GPC ............................................................280
Protein Precipitation Plates (Sirocco) ........................................................................... 80
Polyethylene Glycol (PEG) Aqueous Standard Kit for GPC.......................................280
Protein Isolations and Analyses .......................................................................215-237
Polyethyleneoxide (PEO) Aqueous Standards and Kits for GPC...............................280
Protein-Pak
Polyisoprene Organic Standard Kit for GPC ..............................................................280
Hi-Res Ion-Exchange (IEX) Columns ................................................................223
Polymethylmethacrylate Organic Standard Kits .......................................................280
SEC Columns .....................................................................................................234
Polystyrene (PS) Organic Standards and Kits for GPC .............................................280

PoraPak
HIC Columns......................................................................................................237
PTFE/ETFE Tubing and Fittings ..................................................................................323
Gas Chromatography Packings ........................................................................176
Pullulan Aqueous Standard Kit for GPC ....................................................................280
Porapak RDX Sep-Pak Extraction Cartridge ....................................................... 47
Pumps
PoraPak Rxn Cartridges for Post Synthesis Cleanup ......................................... 56
Common Parts for HPLC Pumps .......................................................................295
Porasil/Porasil
515 HPLC Pump ...............................................................................................296
Bulk Packing .....................................................................................................192
1515 HPLC Pump ............................................................................................296
1525 HPLC Pump ............................................................................................297
1525 micro HPLC Pump ..................................................................................297
Purification and Isolation Cartridge Holders................................184, 186, 188, 190

Preparative Chromatography ............................................................................177-195
Quality Parts and Supplies ................................................................................284-326
Atlantis Preparative OBD Columns..................................................................187
QsertVial Vials ....................................................................................................... 74, 75
Bondapak/Porasil Preparative Columns ..........................................................191
QuEChERS ..................................................................................................................... 54
Delta-Pak Preparative Columns ........................................................................192

How to Scale Up a Separation .........................................................................178
Introduction.......................................................................................................177
Radial Compression Technology
Normal Phase Bulk Packings............................................................................179
Accessories and Spare Parts ............................................................................195
Prep Calculator .................................................................................................180
Prep Nova-Pak HR Preparative Columns ..........................................................191
Radial Compression Modules ...........................................................................193
Preparative Bulk for Lab to Process Scale Purifications .................................192
Radial Compression Column Segments ...........................................................193
RapiGest SF .................................................................................................................254
Preparative OBD Column Technology ..............................................................180
ReadyCal Polystyrene Standards for GPC .................................................................280
Preparative Packing Characteristics.................................................................179
Resolve
Purification and Isolation Cartridge Holders...................................................184
Radial Compression Modules ...........................................................................193
Reversed-Phase Bulk Packings .........................................................................192
Selecting the Column Packing .........................................................................179
Spherisorb 10 m Semipreparative Columns .................................................191
Retention Time Table for Sugars and Carbohydrates................................................169
SunFire Preparative OBD Columns...................................................................185

Symmetry300 Columns for Purification and Isolation ..................................190
SymmetryPrep Columns for Purification and Isolation ..................................190
SAM 1 Reagent for Carbohydrate Analysis ..............................................................170
SymmetryShield Columns for Purification and Isolation................................190
Sample Preparation
XBridge Preparative OBD Columns .................................................................183
Oasis Solid-Phase Extraction (SPE) Products .............................................. 16-38
XSelect Preparative OBD Columns ..................................................................181
Ostro Sample Preparation Products ................................................................... 53
XTerra Preparative OBD Columns ....................................................................189
Sep-Pak Solid-Phase Extraction (SPE) Products .......................................... 39-51
332
Alphabetical Index
www.waters.com
aLPhabETICaL INdEx
HFIP-800 Series Columns for GPC Analysis ..................................................276
15 x 45 mm Vials for Compatible Systems ..................................................... 76
K-800 Series Columns for GPC Analysis ........................................................276
Choosing the Right Vial and Septa for Your Application.................................. 62
KC-811 Columns for Organic Acids................................................................173
GPC 2000 Vials ................................................................................................. 76
KD-800 Series Columns for GPC Analysis .....................................................276
Heat Seal for ACQUITY UPLC Systems ............................................................. 67
KF-800 Series Columns for GPC Analysis ......................................................276
Holder for 12 x 32 mm Vials ..................................................................... 71, 75
KS-Series Columns for Carbohydrate Analysis ...............................................170
Plates for ACQUITY UPLC Systems ................................................................... 66
Protein KW-Series Columns for Gel Filtration .................................................234
Plates for Alliance HT Systems.......................................................................... 69
RSpak Polymer Reversed-Phase Columns ............................................. 162, 164
Screw Cap 12 x 32 mm Vials for ACQUITY UPLC Systems ............................ 64
SC-Series Columns for Carbohydrate Analysis ...............................................170
Screw Cap 12 x 32 mm Vials for Alliance Systems ........................................ 70
SH-1011 Columns for Organic Acids.............................................................173
Screw Cap 12 x 32 mm Vials for Compatible Systems ................................... 74
SP-Series Columns for Carbohydrate Analysis ...............................................170
Settings for the Alliance 2690 and 2695 Vials

and Low Volume Inserts (LVI) ............................................................................. 77
Silica-Pak Cartridges for Carbohydrate Analysis ......................................................170
Snap and Crimp Cap 12 x 32 mm (9 mm Cap)

Vials for Compatible Systems ............................................................................ 75
SLIPFREE Connectors .................................................................................................321
Sirocco Protein Precipitation Filtration Plate............................................................... 80
Snap Cap 12 x 32 mm Vials for Alliance Systems .......................................... 71
Solvent Filtration Apparatus ........................................................................................ 83
Vial Descriptions................................................................................................. 78
Solvent Filtration Membranes ...................................................................................... 83
Vial Selection Guide ........................................................................................... 63
Solvent Inlet Filters .....................................................................................................325
Vials for Waters 717 Autosampler ................................................................... 72
Spherisorb Columns and Accessories........................................................................152
Vials Selector Tool .............................................................................................. 64
Stainless Steel Tubing and Fittings ............................................................................323
Vials Troubleshooting Guide .............................................................................. 79
Styragel
Waters Autosampler Vials.................................................................................. 62

SEC Columns

Introduction.......................................................................................................268
ACQUITY UPLC BEH200 SEC Columns ..........................................................220
HMW High Molecular Weight Columns............................................................274
Protein-Pak and Shodex SEC Columns .............................................................234
HR High-Resolution Columns...........................................................................272
Sentry
HT High-Temperature, High-Stability Columns ...............................................273
Guard Columns Product List..............................................................................164
Solvent Selection Guide ...................................................................................271
Guard Holders Product List ...............................................................................163
Ultrastyragel GPC Columns..............................................................................277
Sep-Pak
96-Well Plates.................................................................................................... 42
Cartridge Accessories......................................................................................... 51
Cartridge Connections Kit .................................................................................. 51
Cartridge Selection Guide .................................................................................. 42
Certified Sep-Pak Solid-Phase Extraction Cartridges .................................. 48-49
Chemistry Selection Chart............................................................................ 42-43
Chemistry Selection Guide ................................................................................. 40
DNPH Silica Cartridges ....................................................................................... 45
Dry SPE Cartridges ............................................................................................. 47
Ozone Scrubber Cartridge.................................................................................. 46
Specialty Chemistries ......................................................................................... 44
Vac RC SPE Cartridges Product List .............................................................. 38-39
Vac SPE Cartridges Product List ................................................................... 38-39
Waters Positive Pressure-96 Processor ...................................................... 38, 51
X PoSure Aldehyde Sampler Cartridges............................................................. 46
Separations Modules
Sugar and Carbohydrate Analysis .............................................................................167

Sugar-Pak 1 Column...................................................................................................170
SunFire Columns
Introduction.......................................................................................................129
Symbiosis Cartridges.................................................................................................... 33
Symmetry
Introduction.......................................................................................................144
Symmetry300
Introduction.......................................................................................................149
2690/2695 Separations Module ...................................................................292
2690D/2695D Separations Module...............................................................292
2790/2795 Separations Module ...................................................................293

2796 Separations Module ...............................................................................293
Supercritical Fluid Chromatography (SFC)
Viridis SFC Columns .........................................................................................196
Shodex
A Guide to Shodex Sugar Columns..................................................................163
www.waters.com
SymmetryPrep
Columns for Purification and Isolation ............................................................190
SymmetryShield
Introduction.......................................................................................................147
Syringe Filters............................................................................................................... 84
Alphabetical Index
333
aLPhabETICaL INdEx
Screw Cap 12 x 32 mm Vials for ACQUITY UPLC Systems ............................ 64

Screw Cap 12 x 32 mm Vials for Alliance Systems ........................................ 70
T3 Columns .................................................................................................................133
Screw Cap 12 x 32 mm Vials for Compatible Systems ................................... 74
T herapeutic Peptide Method Development Kit ..........................................................209
Settings for the Alliance 2690 and 2695 Vials

and Low Volume Inserts (LVI) ............................................................................. 77
Total Recovery Vials and Accessories............................................................ 70, 71, 72

Transition Metal Analysis...........................................................................................175
Snap and Crimp Cap 12 x 32 mm (9 mm Cap)

Vials for Compatible Systems ............................................................................ 75
Trapping Columns
Snap Cap 12 x 32 mm Vials for Alliance Systems .......................................... 71
Atlantis .............................................................................................................259
Symmetry .........................................................................................................259
Vial Descriptions................................................................................................. 78
Symmetry300 ..................................................................................................259
Vial Selection Guide ........................................................................................... 63
SCX ....................................................................................................................259
Vials for Waters 2707 Autosampler................................................................. 73
Triglycerides and Cholesterol Analysis .....................................................................173
Vials for Waters 717 Autosampler ................................................................... 72
Typical Applications for Sugar and Carbohydrate Columns .....................................168
Vials Selector Tool .............................................................................................. 64

Vials Troubleshooting Guide .............................................................................. 79
Tubings and Fittings....................................................................................................323
Waters Autosampler Vials.................................................................................. 62

Viridis SFC Columns ...................................................................................................196
Ultrahydrogel
Aqueous SEC Columns .....................................................................................279
For Sugar and Carbohydrate Analysis .............................................................167
Ultrastyragel
For Cholesterol Analysis ..................................................................................173
UPLC
ACQUITY CSH Columns ..................................................................................... 91
ACQUITY UPLC BEH HILIC Columns .................................................................. 93
ACQUITY UPLC BEH Reversed-Phase Columns ................................................ 93
ACQUITY UPLC Column Ordering Information ................................................. 99
ACQUITY UPLC HSS Columns............................................................................ 95
Heat Seal for ACQUITY UPLC Systems ....................................................67, 105
Introduction......................................................................................................... 90
nanoACQUITY UPLC 2D Kit .............................................................................262
nanoACQUITY UPLC Column Ordering Information.......................................262
Plates for ACQUITY UPLC Systems ..........................................................66, 104
Screw Cap 12 x 32 mm Vials for ACQUITY UPLC Systems ...................64, 103
Settings for ACQUITY UPLC Vials ..................................................................... 65
Vanguard Pre-Columns ..............................................................................96, 102
Waters ACQUITY UPLC System Sample Manager ...................................65, 104
USP L Column Listing .............................................................................................113
W
Waters Global Services...............................................................................................319
Waters Positive Pressure-96 Processor ................................................................ 38, 51
Introduction.......................................................................................................284
ACQUITY UPLC System PM Kits
2996 PDA Detector ...............................................................................285
ACQUITY I2V BSM PM Kit ......................................................................285
Binary Solvent Manager .........................................................................285
ELS Detector............................................................................................285
FLR Detector ...........................................................................................285
Sample Manager.....................................................................................285
Spare Parts & Tubings ............................................................................285
TUV Detector ..........................................................................................285
TUV/PDA/eLambda Detector..................................................................285
ACQUITY UPLC Local Console Controller Kit ..................................................289
Binary & Auxiliary Solvent Manager PM Kit .........................................290
High Pressure BSM/ASM Spares & Tubings ...........................................291
High Pressure SM Spares & Tubings ......................................................291
nanoACQUITY UPLC 2D Spares & Tubing.............................................291
nanoACQUITY UPLC Columns ...............................................................261
Sample Manager PM Kit .........................................................................290
TUV Detector PM Kit ...............................................................................290
TUV Detector Spares & Tubings.............................................................291
Vacuum Manifolds ................................................................................................. 38, 50

Vacuum Pumps .............................................................................................................. 50
Vanguard Pre-Columns ........................................................................................96, 102
Vials and Accessories
15 x 45 mm Vials for Compatible Systems ..................................................... 76
Choosing the Right Vial and Septa for Your Application.................................. 62
ACQUITY UPLC FlexCart ..................................................................................291

Separations Modules
2690/2695 Separations Module .........................................................292
2690D/2695D Separations Module.....................................................292
2790/2795 Separations Module .........................................................293
2796 Separations Module .....................................................................293
GPC 2000 Vials ................................................................................................. 76
Important Information about Component

Selection for Pumps and Separations Modules ...............................................294
Heat Seal for ACQUITY UPLC Systems ............................................................. 67
HPLC Pumps
Holder for 12 x 32 mm Vials ..................................................................... 71, 75
Common Parts for HPLC Pumps .............................................................295
Plates for ACQUITY UPLC Systems ................................................................... 66
515 HPLC Pump .....................................................................................296
Plates for Alliance HT Systems.......................................................................... 69
1515 HPLC Pump ..................................................................................296
334
Alphabetical Index
www.waters.com
aLPhabETICaL INdEx
1525 HPLC Pump ..................................................................................297
General HPLC Parts
1525 micro HPLC Pump ........................................................................297
Column and Cartridge Fittings and Accessories ...................................320
Gradient Modules
Compression Screws and Ferrules .........................................................323
2535 Quarternary Gradient Module..298
PEEK Tubing and Fittings ........................................................................322
2545 Quaternary Gradient Module ......................................................299
PTFE/ETFE Tubing ...................................................................................323
2545/2525 Binary Gradient Module ...................................................298
SLIPFREE Connectors .............................................................................321
Solvent Filters .........................................................................................325
600E Fluid Handling Unit .....................................................................300
Stainless Steel Tubing ............................................................................323
626 LC Fluid Handling Units .................................................................300
Waters Spherisorb Columns and Accessories ..................................................152-154
PrepLC 2000 Pump ................................................................................301
Reagent Manager ...................................................................................301

Injectors /Fraction Collectors
2707 Autosampler ................................................................................302
XBridge
2747 Autosampler ................................................................................303
BEH Technology ................................................................................................122
2757 Sample Manager/Fraction Collector ...........................................303
Column Characteristics ....................................................................................127
2767 Autosampler ................................................................................303
Introduction.......................................................................................................122
2777/2777C Sample Manager ............................................................305
Method Development .......................................................................................124
717plus Autosampler............................................................................302
Manual Injector Rebuild Kits ..................................................................305
Prep OBD Columns .................................................................................. 183-184
Waters Fraction Collector III ...................................................................304
X PoSure Aldehyde Sampler Cartridges....................................................................... 46
Detectors
2414 Refractive Index Detector ............................................................309
XSelect
2424/2420 ELS Detector .....................................................................309
Column Characteristics ....................................................................................118
2465 Electrochemical Detector ............................................................309
CSH Technology .................................................................................................. 91
2475 Multi-Wavelength Fluorescence Detector ...................................310
Introduction.......................................................................................................118
2487/2488 Absorbance Detector .......................................................306
Method Development/Method Transferability .................................................120
2489 Absorbance Detector ..................................................................306

2996 Photodiode Array Detector .........................................................307
432 Conductivity Detector ....................................................................310
Other Systems
Alliance GPC/V 2000 System ...............................................................310

XTerra
Introduction.......................................................................................................140
Oligonucleotide Purification Columns .............................................................244
www.waters.com
Alphabetical Index
335
PaRT NUMbER INdEx

176000861................................ 99
176001519.............................. 258
176001882.............................. 101
176002140................................ 99
186000150.............................. 146
176000862................................ 99
176001556...................... 100, 211
176001883.............................. 101
176002141................................ 99
186000151.............................. 146
176000863................................ 99
176001557...................... 100, 211
176001884.............................. 101
176002142................................ 99
186000152.............................. 146
176000864................................ 99
176001558...................... 100, 211
176001885.............................. 101
176002143................................ 99
186000153.............................. 146
176000874................................ 99
176001559.............................. 100
176001886.............................. 101
176002144................................ 99
186000166.............................. 148
176000875................................ 99
176001560...................... 100, 211
176001887.............................. 101
176002145................................ 99
186000167.............................. 148
176000876................................ 99
176001561...................... 100, 211
176001888.............................. 101
176002146................................ 99
186000168.............................. 148
176000877................................ 99
176001562...................... 100, 211
176001889.............................. 101
176002148................................ 99
186000169...................... 148, 164
176000882................................ 99
176001603.............................. 101
176001890.............................. 101
176002149................................ 99
186000170.............................. 148
176000883................................ 99
176001604.............................. 101
176001903................................ 55
176002150................................ 99
186000171.............................. 148
176000884................................ 99
176001605.............................. 101
176001906.............................. 100
176002151................................ 99
186000172.............................. 148
176000885................................ 99
176001606.............................. 101
176001907.............................. 100
176002152................................ 99
186000173.............................. 148
176000905................................ 99
176001607.............................. 101
176001908.............................. 100
176002153................................ 99
186000174.............................. 148
176000906................................ 99
176001608.............................. 101
176001909.............................. 100
176002154................................ 99
186000175.............................. 148
176000907................................ 99
176001609.............................. 101
176001910.............................. 100
176002155................................ 99
186000176.............................. 148
176000908................................ 99
176001610.............................. 101
176001911.............................. 100
176002156................................ 99
186000177.............................. 148
176001042.............................. 101
176001634................................ 52
176001912.............................. 100
176002157................................ 99
186000178.............................. 148
176001043.............................. 101
176001676................................ 55
176001913.............................. 100
176002158................................ 99
186000179.............................. 148
176001044................................ 99
176001692................................ 52
176001914.............................. 100
176002159................................ 99
186000180.............................. 148
176001045................................ 99
176001740................................ 52
176001915.............................. 100
176002160................................ 99
186000181.............................. 148
176001046................................ 99
176001744................................ 52
176001916.............................. 100
176002161................................ 99
186000182.............................. 148
176001047................................ 99
176001794................................ 99
176001955................................ 52
176002162................................ 99
186000183.............................. 148
176001048................................ 99
176001795................................ 99
176001959.............................. 101
176002163................................ 99
186000185...................... 150, 214
176001049................................ 99
176001796................................ 99
176001960.............................. 101
176002164................................ 99
186000187...................... 150, 214
176001050................................ 99
176001797................................ 99
176001961.............................. 101
176002165................................ 99
186000188...................... 150, 214
176001051................................ 99
176001798................................ 99
176001962.............................. 101
176002166................................ 99
186000189...................... 150, 214
176001089.............................. 100
176001799................................ 99
176001963.............................. 101
176002167................................ 99
186000190...................... 150, 214
176001090.............................. 100
176001800................................ 99
176001964.............................. 101
176002168................................ 99
186000192.............................. 150
176001091.............................. 100
176001801................................ 99
176001965.............................. 101
176002169................................ 99
186000194.............................. 150
176001092.............................. 100
176001802................................ 99
176001966.............................. 101
186000100.............................. 148
186000195.............................. 150
176001093.............................. 100
176001803................................ 99
176002049...................... 314, 316
186000101.............................. 148
186000197...................... 150, 214
176001094.............................. 100
176001804................................ 99
176002050............................ , 313
186000102.............................. 148
186000198...................... 150, 164
176001121...................... 100, 211
176001805................................ 99
176002053.............................. 314
186000103.............................. 148
186000199.............................. 150
176001122...................... 100, 211
176001806................................ 99
176002054.............................. 314
186000104.............................. 148
186000200...................... 150, 214
176001123...................... 100, 211
176001807................................ 99
176002055.............................. 313
186000105.............................. 148
186000201...................... 150, 214
176001124...................... 100, 211
176001808................................ 99
176002058.............................. 315
186000106.............................. 148
186000206.............................. 146
176001125...................... 100, 211
176001809................................ 99
176002059.............................. 315
186000107...................... 148, 164
186000207.............................. 146
176001126...................... 100, 211
176001810.............................. 100
176002060.............................. 317
186000108.............................. 148
186000208.............................. 190
176001127...................... 100, 211
176001811.............................. 100
176002061.............................. 317
186000109.............................. 148
186000209.............................. 190
176001129...................... 100, 211
176001812.............................. 100
176002062.............................. 317
186000110.............................. 148
186000210.............................. 190
176001130...................... 100, 211
176001813.............................. 100
176002063.............................. 317
186000111.............................. 148
186000211.............................. 190
176001131...................... 100, 211
176001814...................... 100, 211
176002064...................... 314, 316
186000112.............................. 148
186000212.............................. 146
176001132...................... 100, 211
176001815...................... 100, 211
176002123.............................. 101
186000113.............................. 148
186000213.............................. 146
176001133...................... 100, 211
176001816...................... 100, 211
176002124.............................. 101
186000114.............................. 148
186000214.............................. 190
176001235.............................. 240
176001817.............................. 100
176002125.............................. 101
186000115.......................... 35, 36
186000215.............................. 190
176001279.............................. 240
176001818...................... 100, 211
176002126.............................. 101
186000116.......................... 35, 36
186000216.............................. 190
176001304................................ 99
176001819...................... 100, 211
176002127.............................. 101
186000117.......................... 35, 36
186000217.............................. 148
176001309................................ 99
176001820...................... 100, 211
176002128.............................. 101
186000118.......................... 35, 36
186000218.............................. 148
176001310................................ 99
176001821.............................. 100
176002129.............................. 101
186000128.......................... 34, 36
186000223.............................. 148
176001311................................ 99
176001822...................... 100, 211
176002130.............................. 101
186000132.......................... 35, 36
186000224.............................. 148
176001367.............................. 262
176001823...................... 100, 211
176002135...................... 314, 316
186000145.............................. 148
186000229.............................. 190
176001375.............................. 100
176001824...................... 100, 211
176002136................................ 99
186000146.............................. 148
186000234................................ 71
176001398.............................. 100
176001835........................ 37, 209
176002137................................ 99
186000147.............................. 148
186000235.............................. 190
176001459.............................. 243
176001836........................ 37, 209
176002138................................ 99
186000148.............................. 146
186000236.............................. 190
176001498.............................. 243
176001881.............................. 101
176002139................................ 99
186000149.............................. 146
186000237.............................. 190
336
Part Number Index
www.waters.com
PaRT NUMbER INdEx

186000327DV ............... 64, 68, 70,
.................................... 73, 74, 103
186000420.............................. 142
186000477.............................. 142
186000532.............................. 142
186000248.......................... 34, 36
186000421.............................. 142
186000478.............................. 142
186000533.............................. 142
186000250.......................... 34, 36
186000328.......................... 71, 75
186000422.............................. 142
186000479.............................. 142
186000534.............................. 142
186000349............ 66, 69, 73, 104
186000423.............................. 142
186000535.............................. 142
186000366.......................... 34, 37
186000480.............................. 142
186000424.............................. 142
186000481.............................. 142
186000536.............................. 142
186000425.............................. 142
186000482.............................. 142
186000537.............................. 142
186000426.............................. 142
186000483.............................. 142
186000538.............................. 141
186000427.............................. 142
186000484.............................. 142
186000539.............................. 141
186000428.............................. 142
186000485.............................. 142
186000540.............................. 141
186000429.............................. 142
186000486.............................. 142
186000541.............................. 141
186000430.............................. 142
186000487.............................. 142
186000542.............................. 141
186000431.............................. 142
186000488.............................. 142
186000543.............................. 141
186000432.............................. 142
186000489.............................. 142
186000544.............................. 141
186000433.............................. 142
186000490.............................. 142
186000545.............................. 141
186000434.............................. 142
186000491.............................. 142
186000546.............................. 141
186000435.............................. 142
186000492.............................. 142
186000547.............................. 141
186000436.............................. 142
186000493.............................. 142
186000548.............................. 141
186000437.............................. 142
186000494.............................. 142
186000549.............................. 141
186000438.............................. 142
186000495.............................. 142
186000550.............................. 141
186000439.............................. 142
186000496.............................. 142
186000551.............................. 141
186000440.............................. 142
186000497.............................. 142
186000552.............................. 141
186000441.............................. 142
186000498.............................. 141
186000553.............................. 141
186000442.............................. 142
186000499.............................. 141
186000554.............................. 142
186000443.............................. 142
186000500.............................. 141
186000555.............................. 142
186000446.............................. 141
186000501.............................. 141
186000556.............................. 142
186000447.............................. 141
186000502.............................. 141
186000557.............................. 142
186000448.............................. 141
186000503.............................. 141
186000558.............................. 142
186000449.............................. 141
186000504.............................. 141
186000559.............................. 142
186000238.............................. 190
186000252.......................... 34, 36
186000253.......................... 34, 36
186000254.......................... 34, 36
186000255.......................... 35, 36
186000256.......................... 35, 36
186000259.......................... 34, 36
186000261.......................... 35, 36
186000262........................ , 36, 38
186000268.............................. 146
186000269.............................. 146
186000270.............................. 146
186000271.............................. 146
186000272C .................. 70, 73, 74
186000272DV ...................... 70, 74
186000273.......................... 70, 74
186000273DV ...................... 70, 74
186000274.......................... 70, 74
186000275...................... 150, 164
186000276.............. 150, 214, 235
186000277.............. 150, 214, 235
186000278.............. 150, 214, 235
186000279.............. 150, 214, 235
186000367.......................... 34, 37
186000368.......................... 34, 37
186000369.......................... 35, 37
186000370.......................... 35, 37
186000371.......................... 35, 37
186000372.......................... 35, 37
186000373.......................... 34, 37
186000375.......................... 34, 37
186000378.......................... 35, 37
186000380.......................... 35, 36
186000381.......................... 35, 36
186000382.......................... 35, 36
186000383.......................... 34, 36
186000384C .............. 240, 243, 70
186000384DV ............................ 70
186000385C ........................ 68, 70
186000385DV ...................... 68, 70
186000386.............................. 141
186000387.............................. 141
186000388.............................. 141
186000389.............................. 141
186000280.............. 150, 214, 235
186000390.............................. 141
186000281.............. 150, 214, 235
186000391.............................. 141
186000282.............. 150, 214, 235
186000392.............................. 141
186000283.............. 150, 214, 235
186000393.............................. 141
186000284...................... 150, 164
186000394.............................. 141
186000285.............. 150, 214, 235
186000395.............................. 141
186000286.............. 150, 214, 235
186000396.............................. 141
186000287.............. 150, 214, 235
186000397.............................. 141
186000288.............. 150, 214, 235
186000398.............................. 141
186000289.............. 150, 214, 235
186000399.............................. 141
186000290.............................. 150
186000400.............................. 141
186000291.............................. 150
186000401.............................. 141
186000292.............................. 150
186000402.............................. 141
186000293.............................. 150
186000403.............................. 141
186000294.............................. 150
186000450.............................. 141
186000505.............................. 141
186000560.............................. 142
186000451.............................. 141
186000506.............................. 141
186000561.............................. 142
186000452.............................. 141
186000507.............................. 141
186000562.............................. 142
186000453.............................. 141
186000508.............................. 141
186000563.............................. 142
186000454.............................. 141
186000509.............................. 141
186000564.............................. 142
186000455.............................. 141
186000510.............................. 142
186000565.............................. 142
186000456.............................. 141
186000511.............................. 142
186000566.............................. 142
186000457.............................. 141
186000512.............................. 142
186000567.............................. 142
186000458.............................. 141
186000513.............................. 142
186000568.............................. 142
186000459.............................. 141
186000514.............................. 142
186000569.............................. 142
186000460.............................. 141
186000515.............................. 142
186000570.............................. 142
186000404.............................. 141
186000461.............................. 141
186000516.............................. 142
186000571.............................. 142
186000295.............................. 150
186000405.............................. 141
186000462.............................. 142
186000517.............................. 142
186000572.............................. 142
186000302................................ 71
186000406.............................. 141
186000463.............................. 142
186000518.............................. 142
186000573.............................. 142
186000302DV ............................ 71
186000407.............................. 141
186000464.............................. 142
186000519.............................. 142
186000574.............................. 142
186000303.......................... 71, 75
186000408.............................. 141
186000465.............................. 142
186000520.............................. 142
186000575.............................. 142
186000409.............................. 141
186000466.............................. 142
186000521.............................. 142
186000576.............................. 142
186000577.............................. 142
186000304.......................... 71, 75
186000305.......................... 70, 74
186000410.............................. 141
186000467.............................. 142
186000522.............................. 142
186000307C ................. 64, 68, 70,

.................................... 73, 74, 103
186000411.............................. 141
186000468.............................. 142
186000523.............................. 142
186000578.............................. 142
186000412.............................. 142
186000469.............................. 142
186000524.............................. 142
186000579.............................. 142
186000307DV ............... 64, 68, 70,

.................................... 73, 74, 103
186000413.............................. 142
186000470.............................. 142
186000525.............................. 142
186000580.............................. 142
186000414.............................. 142
186000471.............................. 142
186000526.............................. 142
186000581.............................. 142
186000309.......................... 34, 36
186000415.............................. 142
186000472.............................. 142
186000527.............................. 142
186000582.............................. 142
186000326C .................. 70, 73, 74
186000416.............................. 142
186000473.............................. 142
186000528.............................. 142
186000583.............................. 142
186000326DV ...................... 70, 74
186000417.............................. 142
186000474.............................. 142
186000529.............................. 142
186000584.............................. 142
186000327C ................. 64, 68, 70,

.................................... 73, 74, 103
186000418.............................. 142
186000475.............................. 142
186000530.............................. 142
186000585.............................. 142
186000419.............................. 142
186000476.............................. 142
186000531.............................. 142
186000588.............................. 142
www.waters.com
Part Number Index
337
PaRT NUMbER INdEx

186000589.............................. 142
186000695.............................. 146
186001168.............................. 189
186000696.............................. 146
186000847DV ............... 64, 68, 70,

.................................... 73, 74, 103
186001033.............................. 189
186000590.............................. 142
186001034.............................. 189
186001169.............................. 189
186000591.............................. 142
186000697.............................. 146
186000848.......................... 70, 74
186001035.............................. 189
186001170.............................. 189
186000592.............................. 141
186000698.............................. 146
186000848DV ...................... 70, 74
186001036.............................. 189
186001171.............................. 189
186000593.............................. 141
186000699.............................. 148
186000855............ 66, 69, 73, 104
186001037.............................. 189
186001172.............................. 189
186000594.............................. 141
186000700.............................. 148
186001104.............................. 189
186001173.............................. 189
186001105.............................. 189
186001174.............................. 189
186001106.............................. 189
186001175.............................. 189
186001107.............................. 189
186001177.............................. 142
186001124................................ 75
186001178.............................. 142
186001125................................ 75
186001179.............................. 141
186001126C .............................. 74
186001180.............................. 141
186001126DV ............................ 74
186001181.............................. 141
186000595.............................. 141
186000701.............................. 148
186000596.............................. 142
186000702.............................. 148
186000597.............................. 142
186000703.............................. 148
186000598.............................. 142
186000704.............................. 148
186000599.............................. 142
186000706................................ 36
186000600.............................. 142
186000708...................... 186, 190
186000601.............................. 142
186000602...................... 142, 143
186000709............. 184, 186, 188,

................................ 190, 208, 211
186000603.............................. 142
186000711.............................. 190
186000632...................... 141, 164
186000712.............................. 190
186000633...................... 141, 164
186000713.............................. 190
186000634...................... 141, 164
186000714.............................. 190
186000635...................... 141, 164
186000715.............................. 190
186000636...................... 141, 164
186000716.............................. 190
186000637...................... 141, 164
186000717.............................. 190
186000638...................... 141, 164
186000718.............................. 190
186000639...................... 141, 164
186000776.......................... 35, 36
186000640...................... 142, 164
186000777.......................... 35, 36
186000641...................... 142, 164
186000778.......................... 35, 36
186000642...................... 142, 164
186000782.......................... 34, 36
186000643...................... 142, 164
186000815.............................. 142
186000644...................... 142, 164
186000816.............................. 142
186000645...................... 142, 164
186000817.............................. 142
186000646...................... 142, 164
186000818.............................. 142
186000647...................... 142, 164
186000826.............................. 143
186000648...................... 141, 164
186000827.............................. 143
186000649...................... 141, 164
186000828.............................. 143
186000650...................... 141, 164
186000829.............................. 143
186000651...................... 141, 164
186000830.............................. 143
186000652...................... 141, 164
186000831.............................. 143
186000653...................... 141, 164
186000832.............................. 143
186000654...................... 141, 164
186000833.............................. 143
186000655...................... 141, 164
186000834.............................. 143
186000656...................... 142, 164
186000835.............................. 143
186000657...................... 142, 164
186000836.............................. 143
186000658...................... 142, 164

186000659...................... 142, 164
186000660...................... 142, 164
186000661...................... 142, 164
186000837C .............................. 72
186000837DV ............................ 72
186000838C ........................ 72, 76
186000838DV ...................... 72, 76
186000856............ 66, 69, 73, 104

186000857............ 66, 69, 73, 104
186000861.............................. 143
186000862.............................. 143
186000863.............................. 143
186000865.......................... 35, 37
186000878.............................. 142
186000879.............................. 142
186000900.............................. 141
186001127................................ 75
186001183.............................. 142
186001128C .............................. 74
186001184.............................. 142
186001128DV ............................ 74
186001185.............................. 141
186001130C .............................. 74
186001186.............................. 141
186001130DV ............................ 74
186001187.............................. 141
186001131C .............................. 74
186001188.............................. 141
186001131DV ............................ 74
186001189.............................. 141
186001133C ........................ 72, 76
186001190.............................. 142
186001133DV ...................... 72, 76
186001191.............................. 142
186001134C ........................ 72, 76
186001192.............................. 141
186001134DV ...................... 72, 76
186001193.............................. 141
186001135.......................... 72, 76
186001194.............................. 142
186001135DV ............................ 76
186001195.............................. 142
186001139.............................. 142
186001196................................ 36
186001140.............................. 142
186001203.............................. 142
186001141.............................. 142
186001204.............................. 142
186001142.............................. 142
186001205.......................... 34, 37
186001143.............................. 142
186001216................................ 35
186001144.............................. 142
186001256.......................... 34, 37
186001145.............................. 142
186001259.............................. 172
186001146.............................. 142
186001260.............................. 172
186001147.............................. 142
186001261.............................. 172
186001148.............................. 142
186001262.............................. 172
186001149.............................. 142
186001263.............................. 172
186001150.............................. 142
186001264.............................. 172
186001151.............................. 142
186001265.............................. 172
186001152.............................. 189
186001279.............................. 137
186001018.............................. 189
186001153.............................. 189
186001281.............................. 137
186001019.............................. 189
186001154.............................. 189
186001283.............................. 137
186001020.............................. 189
186001155.............................. 189
186001285.............................. 137
186001021.............................. 189
186001156.............................. 189
186001287.............................. 137
186001022.............................. 189
186001157.............................. 189
186001289.............................. 137
186001291.............................. 137
186000925.............................. 158
186000926...................... 213, 234
186000965.......................... 72, 76
186000979.............................. 141
186000982.............................. 143
186000984.......................... 71, 75
186000984DV ...................... 71, 75
186000988................................ 36
186000989................................ 70
186000998.............................. 148
186000999.......................... 72, 76
186001001.............................. 189
186001002.............................. 189
186001004.............................. 189
186001005.............................. 189
186001006.............................. 189
186001007.............................. 189
186001008.............................. 189
186001009.............................. 189
186001010.............................. 189
186001011.............................. 189
186001012.............................. 189
186001013.............................. 189
186001014.............................. 189
186001015.............................. 189
186001016.............................. 189
186001017.............................. 189
186000662...................... 142, 164
186000839C ........................ 72, 76
186001023.............................. 189
186001158.............................. 189
186000663...................... 142, 164
186000839DV ...................... 72, 76
186001024.............................. 189
186001159.............................. 189
186001293.............................. 137
186000678.......................... 34, 36
186000840.......................... 72, 76
186001025.............................. 189
186001160.............................. 189
186001295.............................. 137
186000679.......................... 34, 36
186000840DV ............................ 76
186001026.............................. 189
186001161.............................. 189
186001297.............................. 137
186000683.......................... 35, 36
186000841.......................... 72, 76
186001027.............................. 189
186001162.............................. 189
186001299.............................. 137
186000690.............................. 146
186000842.......................... 72, 76
186001028.............................. 189
186001163.............................. 189
186001301.............................. 137
186000691.............................. 146
186000846C .................. 70, 73, 74
186001029.............................. 189
186001164.............................. 189
186001303.............................. 137
186000692.............................. 148
186000846DV ...................... 70, 74
186001030.............................. 189
186001165.............................. 189
186001305.............................. 137
186000693.............................. 148
186000847C ................. 64, 68, 70,

.................................... 73, 74, 103
186001031.............................. 189
186001166.............................. 189
186001307.............................. 137
186001032.............................. 189
186001167.............................. 189
186001309.............................. 137
186000694.............................. 148
338
Part Number Index
www.waters.com
PaRT NUMbER INdEx

186001311.............................. 137
186001480.............................. 189
186001829.................. 209, 34, 37
186001922.............................. 141
186002014.............................. 137
186001313...................... 137, 164
186001481.............................. 189
186001830BA ...................... 34, 36
186001923.............................. 141
186002015.............................. 137
186001315...................... 137, 164
186001482.............................. 189
186001831........................ 209, 38
186001924.............................. 141
186002016.............................. 137
186001317.............................. 137
186001728................................ 72
186001835.............................. 190
186001925.............................. 141
186002017.............................. 137
186001319.............................. 137
186001729.......................... 72, 76
186001836.............................. 190
186001926.............................. 141
186002018.............................. 137
186001321...................... 137, 164
186001741.............................. 275
186001837.............................. 190
186001927.............................. 142
186002019.............................. 137
186001323...................... 137, 164
186001742.............................. 275
186001838.............................. 190
186001930.............................. 189
186002020.............................. 137
186001325.............................. 137
186001743.............................. 275
186001839.............................. 190
186001931.............................. 189
186002021...................... 137, 164
186001327.............................. 137
186001744.............................. 275
186001840.............................. 190
186001932.............................. 189
186002022...................... 137, 164
186001329.............................. 137
186001745.............................. 275
186001841.............................. 190
186001933.............................. 189
186002023...................... 137, 164
186001331.............................. 137
186001746.............................. 275
186001842.............................. 190
186001934.............................. 189
186002024...................... 137, 164
186001333.............................. 137
186001747.............................. 275
186001843.............................. 190
186001935.............................. 189
186002025.............................. 137
186001335.............................. 137
186001749.............................. 275
186001844.............................. 190
186001936.............................. 189
186002026.............................. 137
186001337.............................. 137
186001750.............................. 275
186001845.............................. 190
186001937.............................. 189
186002027.............................. 137
186001340.............................. 137
186001751.............................. 275
186001846.............................. 190
186001938.............................. 189
186002028.............................. 137
186001342.............................. 137
186001752.............................. 275
186001847...................... 190, 214
186001939.............................. 189
186002029.............................. 137
186001344.............................. 137
186001753.............................. 275
186001848...................... 190, 214
186001940.............................. 189
186002030.............................. 137
186001346.............................. 137
186001754.............................. 275
186001849...................... 190, 214
186001941.............................. 189
186002031.............................. 137
186001361.............................. 188
186001755.............................. 275
186001850.............................. 190
186001942.............................. 189
186002032.............................. 137
186001363.............................. 188
186001757.............................. 275
186001855................................ 35
186001943.............................. 189
186002033.............................. 137
186001365.............................. 188
186001758.............................. 275
186001860.............. 252, 257, 263
186001944.............................. 189
186002034................................ 36
186001367.............................. 188
186001759.............................. 275
186001861.............................. 257
186001945.............................. 189
186002035................................ 36
186001369.............................. 188
186001760.............................. 275
186001879.......................... 34, 36
186001972.............................. 142
186002036................................ 36
186001371.............................. 188
186001761.............................. 275
186001880.......................... 34, 36
186001973.............................. 142
186002037................................ 36
186001373.............................. 188
186001762.............................. 275
186001881.......................... 34, 36
186001974.............................. 142
186002038................................ 36
186001375.............................. 188
186001763.............................. 275
186001882.......................... 34, 36
186001975.............................. 142
186002039................................ 36
186001377...................... 137, 164
186001764.............................. 275
186001883.......................... 34, 37
186001976.............................. 142
186002040................................ 36
186001379...................... 137, 164
186001765.............................. 275
186001884.......................... 34, 37
186001977.............................. 142
186002041................................ 36
186001381...................... 137, 164
186001766.............................. 275
186001888................................ 34
186001979.............................. 141
186002042................................ 36
186001383...................... 137, 164
186001767.............................. 275
186001889.............................. 142
186001980.............................. 141
186002043................................ 36
186001385.............................. 137
186001768.............................. 275
186001890.............................. 142
186001982.............................. 141
186002044................................ 36
186001387.............................. 137
186001769.............................. 275
186001891.............................. 142
186001983.............................. 141
186002045................................ 36
186001389.............................. 137
186001770.............................. 275
186001892.............................. 142
186001984.............................. 142
186002046................................ 36
186001391.............................. 137
186001771.............................. 275
186001893.............................. 142
186001985.............................. 142
186002047................................ 36
186001393.............................. 137
186001772.............................. 275
186001894.............................. 142
186001986.............................. 142
186002048................................ 36
186001395.............................. 137
186001773.............................. 275
186001897.............................. 142
186001987.............................. 142
186002049................................ 36
186001413................................ 36
186001774.............................. 275
186001898.............................. 142
186001988.............................. 142
186002051................................ 36
186001414................................ 36
186001775.............................. 275
186001899.............................. 142
186001989.............................. 142
186002052................................ 37
186001420.......................... 73, 76
186001776.............................. 275
186001900.............................. 142
186001990.............................. 142
186002053................................ 37
186001421................................ 76
186001777.............................. 275
186001901.............................. 142
186001991.............................. 142
186002054................................ 37
186001422................................ 76
186001778.............................. 275
186001902.............................. 142
186001992.............................. 142
186002055................................ 37
186001434.............................. 142
186001779.............................. 275
186001906.............................. 141
186001993.............................. 142
186002057................................ 37
186001436............ 66, 69, 73, 104
186001780.............................. 275
186001907.............................. 141
186001994.............................. 142
186002058.............................. 137
186001437............ 66, 69, 73, 104
186001781.............................. 275
186001908.............................. 141
186001995.............................. 142
186002059.............................. 137
186001438............ 66, 69, 73, 104
186001782.............................. 275
186001909.............................. 142
186002003.............................. 137
186002060.............................. 137
186001470.............................. 142
186001783.............................. 275
186001910.............................. 142
186002004.............................. 137
186002061.............................. 137
186001471.............................. 142
186001784.............................. 275
186001911.............................. 142
186002005...................... 137, 164
186002062.............................. 137
186001472.............................. 142
186001785.............................. 275
186001912.............................. 142
186002006...................... 137, 164
186002063.............................. 137
186001473.............................. 142
186001786.............................. 275
186001913.............................. 277
186002007.............................. 137
186002064.............................. 137
186001474.............................. 142
186001787.............................. 275
186001914.............................. 277
186002008.............................. 137
186002065.............................. 137
186001475.............................. 189
186001788.............................. 275
186001915.............................. 277
186002009.............................. 137
186002066.............................. 146
186001476.............................. 189
186001789.............................. 275
186001916.............................. 277
186002010.............................. 137
186002067.............................. 146
186001477.............................. 189
186001790.............................. 275
186001917................................ 42
186002011.............................. 137
186002068.............................. 148
186001478.............................. 189
186001792................................ 36
186001920.............................. 142
186002012.............................. 137
186002069.............................. 148
186001479.............................. 189
186001828BA .............. 34, 36, 249
186001921.............................. 141
186002013.............................. 137
186002070.............................. 146
www.waters.com
Part Number Index
339
PaRT NUMbER INdEx

186002071.............................. 146
186002171.............................. 233
186002258.............................. 189
186002381.............................. 189
186002498.......................... 35, 37
186002072.............................. 148
186002172.............................. 233
186002259.............................. 189
186002382.............................. 189
186002499.................. 209, 34, 37
186002073.............................. 148
186002173.............................. 233
186002260.............................. 189
186002387.............................. 189
186002500.......................... 34, 37
186002074.............................. 146
186002174.............................. 233
186002261.............................. 189
186002388.............................. 189
186002501.......................... 34, 37
186002075.............................. 146
186002175.............................. 233
186002262.............................. 189
186002389.............................. 189
186002502.......................... 34, 37
186002076.............................. 148
186002176.............................. 226
186002263.............................. 189
186002390.............................. 189
186002503.......................... 34, 37
186002077.............................. 148
186002177.............................. 226
186002264.............................. 189
186002417.............................. 188
186002504.......................... 34, 37
186002078.............................. 146
186002178.............................. 226
186002265.............................. 189
186002418.............................. 188
186002505................................ 37
186002079.............................. 146
186002179.............................. 226
186002266.............................. 189
186002425.............................. 212
186002507................................ 37
186002080.............................. 148
186002180.............................. 226
186002267.............................. 189
186002426.............................. 212
186002508................................ 37
186002081.............................. 148
186002181.............................. 226
186002268.............................. 189
186002427.............................. 212
186002509................................ 37
186002082.............................. 146
186002182...................... 213, 226
186002269.............................. 189
186002428.............................. 212
186002520.......................... 72, 76
186002083.............................. 146
186002183...................... 213, 226
186002270.............................. 189
186002429.............................. 212
186002526.............................. 131
186002084.............................. 148
186002184...................... 213, 226
186002271.............................. 189
186002430.............................. 212
186002528.............................. 131
186002085.............................. 148
186002185...................... 213, 226
186002272.............................. 189
186002431.............................. 212
186002529.............................. 131
186002086.............................. 146
186002186.............................. 226
186002273.............................. 189
186002432.............................. 212
186002187.............................. 226
186002274.............................. 189
186002433.............................. 212
186002530...................... 131, 164
186002087.............................. 146
186002088.............................. 148
186002188...................... 151, 260
186002275.............................. 189
186002089.............................. 148
186002189...................... 151, 260
186002276.............................. 189
186002090.............................. 146
186002190...................... 151, 260
186002277.............................. 189
186002091.............................. 146
186002191...................... 151, 260
186002278.............................. 189
186002092.............................. 148
186002192...................... 151, 260
186002279.............................. 189
186002093.............................. 148
186002193...................... 151, 260
186002280.............................. 189
186002094.............................. 146
186002194.............................. 260
186002281.............................. 189
186002095.............................. 146
186002195.............................. 260
186002282.............................. 189
186002096.............................. 148
186002197.............................. 260
186002298.............................. 188
186002097.............................. 148
186002198.............................. 141
186002299.............................. 188
186002098................................ 36
186002199.............................. 141
186002300.............................. 188
186002099................................ 37
186002200.............................. 141
186002311.............................. 137
186002118.............................. 257
186002201...................... 151, 260
186002312.............................. 137
186002122.............................. 257
186002202...................... 151, 260
186002313.............................. 137
186002123.............................. 257
186002203...................... 151, 260
186002314.............................. 137
186002127.......................... 70, 74
186002206...................... 151, 260
186002315.............................. 137
186002128.......................... 70, 74
186002208.............................. 260
186002316.............................. 137
186002129.......................... 70, 74
186002209.............................. 260
186002318................................ 43
186002130.......................... 70, 74
186002210.............................. 141
186002319................................ 42
186002152.............................. 236
186002211.............................. 141
186002320................................ 42
186002153.............................. 236
186002212.............................. 141
186002321................................ 42
186002154.............................. 236
186002213.............................. 260
186002325...................... 255, 263
186002155.............................. 236
186002214.............................. 260
186002326.............................. 255
186002156.............................. 236
186002218.............................. 189
186002327.............................. 255
186002157.............................. 236
186002219.............................. 189
186002328.............................. 255
186002158.............................. 236
186002220.............................. 189
186002329.............................. 255
186002159.............................. 237
186002221.............................. 189
186002330...................... 255, 262
186002160.............................. 237
186002222.............................. 189
186002337.............................. 254
186002483................... 66, 69, 73,

.......................... 209, 73, 104, 104
186002161.............................. 233
186002223.............................. 189
186002338.............................. 254
186002484............ 66, 69, 73, 104
186002560.............................. 131
186002162.............................. 233
186002224.............................. 189
186002344................................ 99
186002489.......................... 34, 37
186002561.............................. 186
186002163.............................. 233
186002225.............................. 189
186002346................................ 99
186002490.......................... 34, 37
186002562.............................. 186
186002164.............................. 233
186002234.............................. 143
186002347................................ 99
186002491.......................... 34, 37
186002563.............................. 186
186002165.............................. 233
186002235.............................. 143
186002349................................ 99
186002492.......................... 34, 37
186002564.............................. 186
186002166.............................. 233
186002236.............................. 143
186002350................................ 99
186002493.......................... 35, 37
186002565.............................. 186
186002167.............................. 233
186002254.............................. 189
186002352................................ 99
186002494.......................... 34, 37
186002566.............................. 186
186002168.............................. 233
186002255.............................. 189
186002353................................ 99
186002495.......................... 34, 37
186002567.............................. 186
186002169.............................. 233
186002256.............................. 189
186002379.............................. 189
186002496.......................... 34, 37
186002568.............................. 186
186002170.............................. 233
186002257.............................. 189
186002380.............................. 189
186002497.......................... 34, 37
186002569.............................. 186
340
Part Number Index
186002434.............................. 212
186002435.............................. 212
186002436.............................. 212
186002437.............................. 212
186002438.............................. 212
186002439.............................. 212
186002440.............................. 212
186002448........................ 80, 105
186002452.............................. 188
186002453.............................. 188
186002454.............................. 188
186002455.............................. 188
186002456.............................. , 70
186002457.............................. , 70
186002459...................... 151, 260
186002461...................... 151, 260
186002462...................... 151, 260
186002463...................... 151, 260
186002464...................... 151, 260
186002466.............................. 260
186002467.............................. 260
186002468.............................. 260
186002469.............................. 141
186002470.............................. 141
186002471.............................. 141
186002472.............................. 260
186002481.............................. 209
186002531.............................. 131
186002532.............................. 131
186002533.............................. 131
186002534.............................. 131
186002535.............................. 131
186002536...................... 131, 164
186002537.............................. 131
186002538.............................. 131
186002539.............................. 131
186002540.............................. 131
186002541.............................. 131
186002542.............................. 131
186002543.............................. 131
186002544.............................. 131
186002545.............................. 131
186002546.............................. 131
186002547.............................. 131
186002548.............................. 131
186002549.............................. 131
186002550.............................. 131
186002551.............................. 131
186002552.............................. 131
186002553.............................. 131
186002554.............................. 131
186002555.............................. 131
186002556.............................. 131
186002557.............................. 131
186002558.............................. 131
186002559.............................. 131
www.waters.com
PaRT NUMbER INdEx

186002570.............................. 186
186002649.......................... 71, 75
186002727...................... 131, 164
186002806.............................. 259
186002995.............................. 184
186002571.............................. 186
186002650.......................... 71, 75
186002728.............................. 131
186002808.............................. 259
186002996.............................. 184
186002572.............................. 186
186002663.............................. 186
186002729.............................. 131
186002817.............................. 252
186002997.............................. 184
186002574.............................. 259
186002664.............................. 186
186002730.............................. 131
186002843.............................. 189
186002999.............................. 128
186002581...................... 151, 260
186002665.............................. 186
186002731.............................. 131
186002844.............................. 189
186003002.............................. 128
186002582...................... 151, 260
186002666.............................. 186
186002732.............................. 131
186002845.............................. 189
186003003.............................. 128
186002583...................... 151, 260
186002667.............................. 186
186002733...................... 131, 164
186002846.............................. 189
186003004.............................. 128
186002584...................... 151, 260
186002668.............................. 186
186002734.............................. 131
186002847.............................. 189
186003005.............................. 128
186002585...................... 151, 260
186002669.............................. 186
186002735.............................. 131
186002848.............................. 189
186003006.............................. 128
186002586...................... 151, 260
186002670.............................. 186
186002736.............................. 131
186002849.............................. 189
186003007.............................. 128
186002587...................... 151, 260
186002671.............................. 186
186002737.............................. 131
186002850.............................. 189
186003008.............................. 128
186002588...................... 151, 260
186002672.............................. 186
186002738.............................. 131
186002851................................ 99
186003009.............................. 128
186002589...................... 151, 260
186002673.............................. 186
186002739.............................. 131
186002852................................ 99
186003010.............................. 128
186002590...................... 151, 260
186002674.............................. 131
186002740.............................. 131
186002853................................ 99
186003011.............................. 128
186002591...................... 151, 260
186002675.............................. 131
186002741.............................. 131
186002854................................ 99
186003012.............................. 128
186002592...................... 151, 260
186002676.............................. 131
186002742.............................. 131
186002865...................... 256, 263
186003013.............................. 128
186002677.............................. 131
186002743.............................. 131
186002866...................... 256, 263
186003014.............................. 128
186002678.............................. 131
186002744.............................. 131
186002867.............................. 186
186003015.............................. 128
186002679.............................. 131
186002745.............................. 131
186002868.............................. 186
186003016.............................. 128
186002680.............................. 131
186002746.............................. 186
186002869.............................. 186
186003017.............................. 128
186002681...................... 131, 164
186002747.............................. 186
186002870.............................. 186
186003019.............................. 127
186002682...................... 131, 164
186002748.............................. 186
186002871.............................. 186
186003020.............................. 127
186002683...................... 131, 164
186002749.............................. 186
186002872.............................. 186
186003021.............................. 127
186002684...................... 131, 164
186002750.............................. 186
186002875................................ 99
186003022.............................. 127
186002685.............................. 131
186002751.............................. 186
186002876................................ 99
186003023.............................. 127
186002686.............................. 131
186002752.............................. 186
186002877................................ 99
186003024.............................. 127
186002687.............................. 259
186002753.............................. 186
186002878................................ 99
186003025.............................. 127
186002697.............................. 131
186002754.............................. 186
186002882................................ 99
186003026.............................. 127
186002698.............................. 131
186002755.............................. 186
186002883................................ 99
186003027.............................. 127
186002699.............................. 131
186002756.............................. 186
186002884................................ 99
186003028.............................. 127
186002700.............................. 131
186002757.............................. 186
186002885................................ 99
186003029.............................. 128
186002701.............................. 131
186002758.............................. 186
186002892................................ 37
186003030.............................. 128
186002702.............................. 131
186002759.............................. 186
186002893................................ 37
186003031.............................. 128
186002705.............................. 131
186002760.............................. 186
186002972.............................. 184
186003032.............................. 128
186002706.............................. 131
186002761.............................. 186
186002973.............................. 184
186003033.............................. 128
186002707.............................. 131
186002762.............................. 186
186002974.............................. 184
186003034.............................. 128
186002708...................... 131, 164
186002763.............................. 186
186002975.............................. 184
186003035.............................. 127
186002709.............................. 131
186002764.............................. 186
186002977.............................. 184
186003036.............................. 127
186002710.............................. 131
186002765.............................. 186
186002978.............................. 184
186003037.............................. 127
186002711.............................. 131
186002766.............................. 186
186002979.............................. 184
186003038.............................. 127
186002712.............................. 131
186002767.............................. 186
186002980.............................. 184
186003039.............................. 127
186002713...................... 131, 164
186002768.............................. 186
186002981.............................. 184
186003040.............................. 127
186002714.............................. 131
186002780.............................. 252
186002982.............................. 184
186003041.............................. 127
186002715.............................. 131
186002785.............................. 258
186002983.............................. 184
186003042.............................. 128
186002716.............................. 131
186002786.................. 67, 69, 105
186002984.............................. 184
186003043.............................. 128
186002633................................ 69
186002717.............................. 131
186002787.................. 67, 69, 105
186002985.............................. 184
186003044.............................. 128
186002639................... 64, 68, 70,

.................................... 73, 74, 103
186002718...................... 131, 164
186002788............ 66, 69, 73, 104
186002986.............................. 184
186003045.............................. 128
186002719.............................. 131
186002789............ 66, 69, 73, 104
186002987.............................. 184
186003046.............................. 127
186002640.................... 70, 73, 74
186002720.............................. 131
186002795.............................. 186
186002988.............................. 184
186003047.............................. 127
186002641.......................... 71, 75
186002721.............................. 131
186002797.............................. 186
186002989.............................. 184
186003048.............................. 127
186002642.......................... 71, 75
186002722...................... 131, 164
186002800.............................. 188
186002990.............................. 184
186003049.............................. 127
186002645.......................... 71, 75
186002723.............................. 131
186002801.............................. 188
186002991.............................. 184
186003050.............................. 127
186002646.......................... 71, 75
186002724.............................. 131
186002802.......................... 70, 74
186002992.............................. 184
186003051.............................. 127
186002647.......................... 71, 75
186002725.............................. 131
186002803................................ 74
186002993.............................. 184
186003052.............................. 127
186002648.......................... 71, 75
186002726.............................. 131
186002804................................ 74
186002994.............................. 184
186003053.............................. 128
186002593.............................. 260
186002594.............................. 260
186002595.............................. 260
186002596.............................. 260
186002597.............................. 260
186002598.............................. 260
186002599.............................. 141
186002600.............................. 141
186002601.............................. 141
186002602.............................. 141
186002603.............................. 141
186002604.............................. 141
186002605.............................. 260
186002608.............................. 146
186002609.............................. 146
186002611.............................. 148
186002612.............................. 146
186002613.............................. 146
186002614.............................. 148
186002615.............................. 148
186002616.............................. 146
186002617.............................. 146
186002618.............................. 148
186002619.............................. 148
186002622.............................. 259
186002623.............................. 259
186002626.......................... 70, 74
186002628.......................... 71, 75
186002629C ........................ 72, 76
186002630C ........................ 72, 76
www.waters.com
Part Number Index
341
PaRT NUMbER INdEx

186003054.............................. 128
186003124.............................. 127
186003188.............................. 128
186003326.............................. 127
186003395...................... 131, 164
186003055.............................. 128
186003126.............................. 127
186003189.............................. 128
186003327.............................. 127
186003396...................... 131, 164
186003056...................... 127, 164
186003127.............................. 127
186003190.............................. 128
186003328.............................. 127
186003397.............................. 131
186003057...................... 127, 164
186003128.............................. 127
186003191.............................. 128
186003329.............................. 127
186003398.............................. 131
186003058...................... 127, 164
186003129.............................. 128
186003192.............................. 128
186003330.............................. 128
186003399.............................. 131
186003059...................... 127, 164
186003130.............................. 128
186003193.............................. 128
186003331.............................. 128
186003400.............................. 131
186003060...................... 127, 164
186003131.............................. 128
186003194.............................. 128
186003332.............................. 128
186003401.............................. 131
186003061...................... 128, 164
186003132.............................. 128
186003195.............................. 128
186003333.............................. 128
186003402.............................. 131
186003062...................... 128, 164
186003133.............................. 128
186003201.............................. 127
186003334.............................. 128
186003403.............................. 131
186003063...................... 128, 164
186003134.............................. 128
186003249.......................... 35, 37
186003335.............................. 128
186003404.............................. 131
186003064...................... 128, 164
186003135.............................. 128
186003254.............................. 131
186003336.............................. 128
186003405...................... 131, 164
186003065...................... 127, 164
186003136.............................. 127
186003255.............................. 184
186003337.............................. 128
186003406...................... 131, 164
186003066...................... 127, 164
186003139.............................. 127
186003256.............................. 184
186003338.............................. 128
186003407.............................. 131
186003067...................... 127, 164
186003140.............................. 127
186003257.............................. 184
186003339.............................. 128
186003408.............................. 131
186003068...................... 127, 164
186003141.............................. 127
186003258.............................. 184
186003340.............................. 128
186003409.............................. 131
186003069...................... 127, 164
186003142.............................. 127
186003259.............................. 184
186003341.............................. 128
186003410.............................. 131
186003070...................... 128, 164
186003144.............................. 127
186003260.............................. 184
186003342.............................. 128
186003411.............................. 131
186003071...................... 128, 164
186003145.............................. 127
186003262.............................. 184
186003343.............................. 128
186003412.............................. 131
186003072...................... 128, 164
186003146.............................. 127
186003264.............................. 184
186003344.............................. 128
186003413...................... 131, 164
186003073...................... 128, 164
186003148.............................. 127
186003265.............................. 184
186003345.............................. 128
186003414...................... 131, 164
186003074...................... 127, 164
186003149.............................. 127
186003266.............................. 184
186003346.............................. 128
186003415.............................. 131
186003075...................... 127, 164
186003150.............................. 127
186003267.............................. 184
186003347.............................. 128
186003416.............................. 131
186003076...................... 127, 164
186003151.............................. 127
186003269.............................. 184
186003348.............................. 128
186003417.............................. 131
186003077...................... 127, 164
186003152.............................. 127
186003271.............................. 184
186003349.............................. 128
186003418.............................. 131
186003078...................... 127, 164
186003153.............................. 127
186003272.............................. 184
186003350.............................. 128
186003419.............................. 131
186003079...................... 128, 164
186003154.............................. 128
186003273.............................. 184
186003351.............................. 128
186003420.............................. 131
186003080...................... 128, 164
186003155.............................. 128
186003274.............................. 184
186003352.............................. 128
186003425.............................. 186
186003081...................... 128, 164
186003156.............................. 128
186003276.............................. 184
186003353.............................. 128
186003426.............................. 186
186003082...................... 128, 164
186003157.............................. 128
186003277.............................. 184
186003354.............................. 184
186003427.............................. 186
186003083.............................. 184
186003158.............................. 128
186003278.............................. 184
186003355.............................. 184
186003428.............................. 186
186003084.............................. 127
186003159.............................. 128
186003279.............................. 184
186003356.............................. 184
186003429.............................. 186
186003085.............................. 127
186003160.............................. 128
186003284.............................. 184
186003357.............................. 184
186003431.............................. 186
186003087.............................. 127
186003161.............................. 128
186003285.............................. 255
186003358.............................. 184
186003432.............................. 186
186003088.............................. 127
186003162.............................. 128
186003286...................... 255, 263
186003359...................... 127, 164
186003433.............................. 186
186003089.............................. 127
186003163.............................. 128
186003287.............................. 255
186003360...................... 127, 164
186003434.............................. 186
186003090.............................. 127
186003164.............................. 127
186003306.............................. 127
186003361...................... 127, 164
186003435.............................. 186
186003091.............................. 127
186003167.............................. 127
186003307.............................. 127
186003362...................... 127, 164
186003436.............................. 186
186003092.............................. 127
186003168.............................. 127
186003308.............................. 127
186003363...................... 127, 164
186003437.............................. 186
186003096.............................. 127
186003169.............................. 127
186003309.............................. 127
186003364...................... 128, 164
186003438.............................. 186
186003099.............................. 127
186003170.............................. 127
186003310.............................. 127
186003365.......................... 35, 36
186003439.............................. 186
186003101.............................. 127
186003172.............................. 127
186003311.............................. 127
186003366...................... 128, 164
186003440.............................. 186
186003107.............................. 128
186003173.............................. 127
186003312.............................. 127
186003367...................... 128, 164
186003441.............................. 186
186003108.............................. 128
186003174.............................. 127
186003313.............................. 127
186003368...................... 128, 164
186003442.............................. 186
186003109.............................. 128
186003175.............................. 127
186003314.............................. 127
186003369................................ 44
186003443.............................. 186
186003110.............................. 128
186003177.............................. 127
186003315.............................. 127
186003373................................ 99
186003444.............................. 186
186003111.............................. 128
186003178.............................. 127
186003316.............................. 127
186003374................................ 99
186003445.............................. 186
186003112.............................. 128
186003179.............................. 127
186003317.............................. 127
186003375................................ 99
186003446.............................. 186
186003113.............................. 128
186003180.............................. 127
186003318.............................. 127
186003376................................ 99
186003447.............................. 186
186003114.............................. 128
186003181.............................. 127
186003319.............................. 127
186003377................................ 99
186003448.............................. 186
186003115.............................. 128
186003182.............................. 127
186003320.............................. 127
186003378................................ 99
186003449.............................. 186
186003116.............................. 128
186003183.............................. 128
186003321.............................. 127
186003379.......................... 35, 36
186003450.............................. 186
186003117.............................. 128
186003184.............................. 128
186003322.............................. 127
186003390.............................. 186
186003451.............................. 186
186003118.............................. 127
186003185.............................. 128
186003323.............................. 127
186003391.............................. 186
186003452.............................. 186
186003121.............................. 127
186003186.............................. 128
186003324.............................. 127
186003392.............................. 131
186003453.............................. 186
186003122.............................. 127
186003187.............................. 128
186003325.............................. 127
186003394.............................. 131
186003454.............................. 186
342
Part Number Index
www.waters.com
PaRT NUMbER INdEx

186003455................................ 70
186003556.............................. 207
186003618.............................. 208
186003677.............................. 208
186003741.............................. 137
186003457.............................. 100
186003560.............................. 207
186003619.............................. 208
186003682.............. 260, 261, 207
186003742.............................. 137
186003458.............................. 100
186003561.............................. 207
186003620.............................. 208
186003685.............. 248, 209, 207
186003743.............................. 137
186003459.............................. 100
186003562.............................. 207
186003621.............................. 208
186003686.............................. 207
186003744.............................. 137
186003460.............................. 100
186003563.............................. 207
186003622.............................. 208
186003687.............................. 207
186003745.............................. 137
186003461.............................. 100
186003564.............................. 207
186003623.............................. 208
186003691...................... 188, 211
186003746.............................. 137
186003462.............................. 100
186003565.............................. 207
186003624.............................. 208
186003692...................... 188, 211
186003747.............................. 137
186003463.......................... 35, 37
186003566.............................. 207
186003625.............................. 208
186003693...................... 188, 211
186003748.............................. 137
186003467.............................. 186
186003567.............................. 207
186003626.............................. 208
186003694...................... 188, 211
186003751.............................. 137
186003468.............................. 186
186003568.............................. 207
186003627.............................. 208
186003695...................... 188, 211
186003754.............................. 137
186003491.............................. 262
186003569.............................. 207
186003628.............................. 208
186003696...................... 188, 211
186003755.............................. 137
186003492.............................. 262
186003570.............................. 207
186003629.............................. 208
186003697...................... 188, 211
186003756...................... 137, 164
186003493.............................. 262
186003571.............................. 207
186003630.............................. 208
186003698...................... 188, 211
186003757...................... 137, 164
186003494.............................. 262
186003572.............................. 207
186003631.............................. 208
186003699...................... 188, 211
186003758...................... 137, 164
186003495.............................. 262
186003573.............................. 207
186003632.............................. 207
186003700...................... 188, 211
186003759...................... 137, 164
186003496.............................. 262
186003574.............................. 207
186003633.............................. 207
186003701...................... 188, 211
186003760...................... 137, 164
186003497.............................. 262
186003575.............................. 207
186003634.............................. 207
186003702...................... 188, 211
186003761...................... 137, 164
186003498.............................. 262
186003576.............................. 207
186003635.............................. 207
186003703...................... 188, 211
186003766.............................. 128
186003499.............................. 262
186003577.............................. 207
186003636.............................. 207
186003704...................... 188, 211
186003767.............................. 128
186003500...................... 262, 263
186003578.............................. 207
186003637.............................. 207
186003705...................... 188, 211
186003768.............................. 128
186003501.............................. 262
186003579.............................. 207
186003638.............................. 207
186003706...................... 188, 211
186003769.............................. 128
186003502.............................. 262
186003580.............................. 207
186003639.............................. 207
186003707...................... 188, 211
186003770.............................. 128
186003503.............................. 262
186003581.............................. 207
186003640.............................. 207
186003708...................... 188, 211
186003771.............................. 128
186003504.............................. 262
186003582.............................. 208
186003641.............................. 207
186003709...................... 188, 211
186003772.............................. 128
186003505.............................. 262
186003583.............................. 208
186003642.............................. 207
186003710...................... 188, 211
186003773.............................. 128
186003506...................... 262, 263
186003584.............................. 208
186003643.............................. 207
186003711...................... 188, 211
186003774.............................. 128
186003507.............................. 262
186003585.............................. 208
186003648.............................. 208
186003712...................... 188, 211
186003775.............................. 128
186003513.............................. 262
186003586.............................. 208
186003649.............................. 208
186003713...................... 137, 211
186003776.............................. 128
186003514.................... , 262, 263
186003587.............................. 208
186003650.............................. 208
186003714...................... 137, 211
186003777.............................. 128
186003516.......................... 35, 36
186003588.............................. 207
186003651.............................. 208
186003715.............................. 137
186003778.............................. 128
186003517.......................... 35, 37
186003589.............................. 207
186003652.............................. 208
186003716.............................. 137
186003779.............................. 128
186003518.......................... 35, 37
186003590.............................. 207
186003653.............................. 208
186003717...................... 137, 211
186003780.............................. 128
186003519.......................... 35, 37
186003591.............................. 207
186003654.............................. 208
186003718...................... 137, 211
186003781.............................. 128
186003522........................ 209, 38
186003592.............................. 207
186003655.............................. 208
186003719...................... 137, 211
186003782.............................. 128
186003524........................ 83, 105
186003593.............................. 207
186003656.............................. 208
186003720.............................. 137
186003783.............................. 128
186003529...................... 100, 211
186003594.............................. 207
186003657.............................. 208
186003721...................... 137, 211
186003784.............................. 128
186003530...................... 100, 211
186003595.............................. 207
186003658.............................. 208
186003722...................... 137, 211
186003785.............................. 128
186003531...................... 100, 211
186003596.............................. 207
186003659.............................. 208
186003723...................... 137, 211
186003786.............................. 128
186003532...................... 100, 211
186003597.............................. 207
186003660.............................. 208
186003724.............................. 137
186003787.............................. 128
186003533...................... 100, 211
186003598.............................. 207
186003661.............................. 208
186003725.............................. 137
186003788.............................. 128
186003534...................... 100, 211
186003599.............................. 207
186003662.............................. 208
186003726.............................. 137
186003789.............................. 128
186003535...................... 100, 211
186003604.............................. 207
186003663.............................. 208
186003727.............................. 137
186003790.............................. 128
186003536...................... 100, 211
186003605.............................. 207
186003664.............................. 208
186003728.............................. 137
186003791.............................. 128
186003537...................... 100, 211
186003606.............................. 207
186003665.............................. 208
186003729.............................. 137
186003792.............................. 128
186003538...................... 100, 211
186003607...................... 207, 209
186003666.............................. 208
186003730...................... 137, 211
186003793.............................. 128
186003539...................... 100, 211
186003608.............................. 207
186003667.............................. 208
186003731...................... 137, 211
186003794.............................. 128
186003540...................... 100, 211
186003609.............................. 207
186003668.............................. 208
186003732.............................. 137
186003795.............................. 128
186003542...................... 262, 207
186003610.............................. 207
186003669.............................. 208
186003733.............................. 137
186003796.............................. 128
186003543...................... 207, 262
186003611.............................. 207
186003670.............................. 208
186003734...................... 137, 211
186003797.............................. 128
186003544...................... 207, 262
186003612.............................. 207
186003671.............................. 208
186003735...................... 137, 211
186003798.............................. 128
186003545...................... 207, 262
186003613.............................. 207
186003672.............................. 208
186003736...................... 137, 211
186003799.............................. 128
186003546...................... 207, 262
186003614.............................. 207
186003673.............................. 208
186003737.............................. 137
186003800.............................. 128
186003550...................... 207, 262
186003615.............................. 207
186003674.............................. 208
186003738...................... 137, 211
186003801.............................. 128
186003554.............................. 207
186003616.............................. 208
186003675.............................. 208
186003739...................... 137, 211
186003802.............................. 128
186003555.............................. 207
186003617.............................. 208
186003676.............................. 208
186003740...................... 137, 211
186003803.............................. 128
www.waters.com
Part Number Index
343
PaRT NUMbER INdEx

186003804.............................. 128
186003933.............................. 184
186004001.............................. 184
186004107.............................. 184
186004464.............................. 208
186003805.............................. 128
186003934.............................. 184
186004002.............................. 184
186004108................................ 76
186004465.............................. 208
186003806.............................. 128
186003935.............................. 184
186004003.............................. 184
186004109................................ 76
186004466.............................. 208
186003807.............................. 128
186003936.............................. 184
186004004.............................. 184
186004111................................ 76
186004467.............................. 208
186003808.............................. 128
186003937.............................. 184
186004005.............................. 184
186004112.................. 64, 70, 103
186004468.............................. 208
186003809.............................. 128
186003938.............................. 184
186004006.............................. 184
186004114...................... 100, 211
186004469.............................. 208
186003810.............................. 207
186003939.............................. 184
186004007.............................. 184
186004115...................... 100, 211
186004470.............................. 208
186003811.............................. 207
186003940.............................. 184
186004008.............................. 184
186004116...................... 100, 211
186004471.............................. 208
186003812.............................. 207
186003941.............................. 186
186004009.............................. 184
186004117.............................. 100
186004475.......................... 35, 37
186003813.............................. 207
186003943.............................. 128
186004010.............................. 184
186004118...................... 100, 211
186004487.......................... 71, 75
186003814.............................. 207
186003944.............................. 100
186004011.............................. 184
186004119...................... 100, 211
186004495.............................. 222
186003815.............................. 207
186003945.............................. 208
186004012.............................. 184
186004120...................... 100, 211
186004496.............................. 222
186003820.............................. 263
186003946.............................. 208
186004013.............................. 184
186004132C .......... 64, 68, 70, 103
186004497.............................. 222
186003821.............................. 263
186003949.............................. 246
186004014.............................. 184
186004133C ................ 64, 70, 103
186004498.............................. 222
186003836.............................. 240
186003950.............................. 246
186004015.............................. 184
186004134.......................... 34, 36
186004499.............................. 222
186003837.............................. 240
186003951.............................. 246
186004016.............................. 184
186004135...................... 256, 249
186004500.............................. 222
186003952.............................. 246
186004021.............................. 184
186004136.............................. 102
186004501.............................. 222
186003953.............................. 246
186004022.............................. 184
186004137.............................. 102
186004502.............................. 222
186003954.............................. 246
186004023.............................. 184
186004138.............................. 102
186004503.............................. 222
186003955.............................. 246
186004024.............................. 184
186004167C .............................. 70
186004504.............................. 222
186003956.............................. 188
186004025.............................. 184
186004168C ................ 64, 70, 103
186004505.............................. 222
186003957.............................. 188
186004026...................... 188, 211
186004169.......................... 70, 74
186004506.............................. 222
186003958.............................. 188
186004027.............................. 186
186004429.............................. 127
186004536................................ 43
186003959.............................. 188
186004028.............................. 186
186004430.............................. 127
186004537................................ 43
186003960.............................. 188
186004029.............................. 186
186004431.............................. 127
186004538................................ 42
186003961.............................. 188
186004030.............................. 188
186004432.............................. 127
186004541................................ 61
186003962.............................. 188
186004031.......................... 72, 76
186004433.............................. 127
186004542................................ 61
186003963.............................. 128
186004032.............................. 258
186004434.............................. 127
186004543................................ 61
186003964.............................. 128
186004044.............................. 102
186004435.............................. 127
186004544................................ 61
186003965.............................. 128
186004045.............................. 102
186004436.............................. 127
186004545................................ 61
186003966................................ 42
186004046.............................. 102
186004437.............................. 128
186004546................................ 61
186003969.............................. 186
186004047.............................. 102
186004438.............................. 128
186004547................................ 61
186003970.............................. 186
186004048.............................. 102
186004439.............................. 128
186004548................................ 61
186003971.............................. 186
186004049.............................. 102
186004440.............................. 128
186004560................................ 42
186003972.............................. 186
186004050.............................. 102
186004441.............................. 128
186004561................................ 42
186003975...................... 102, 207
186004051................................ 44
186004442.............................. 128
186004562................................ 42
186003976.............................. 102
186004052.............................. 102
186004443.............................. 128
186004563................................ 43
186003977.............................. 102
186004053.............................. 102
186004444.............................. 128
186004564................................ 43
186003978.............................. 102
186004054.............................. 102
186004445.............................. 128
186004565................................ 43
186003979.............................. 102
186004055.............................. 102
186004446.............................. 128
186004566.......................... 43, 84
186003899.............................. 184
186003980.............................. 102
186004056.............................. 102
186004447.............................. 128
186004567.......................... 42, 84
186003900.............................. 184
186003981.............................. 102
186004057.............................. 102
186004448.............................. 128
186004568................................ 43
186003901.............................. 184
186003987.............................. 100
186004058.............................. 102
186004449.............................. 128
186004569................................ 61
186003902.............................. 184
186003988.............................. 184
186004080...................... 188, 211
186004450.............................. 128
186004570................................ 61
186003903.............................. 263
186003989.............................. 184
186004081...................... 188, 211
186004451.............................. 128
186004571................................ 55
186003904.............................. 263
186003990.............................. 184
186004082...................... 188, 211
186004452.............................. 128
186004572................................ 55
186003908................................ 36
186003991.............................. 184
186004083...................... 188, 211
186004453.............................. 128
186004578................................ 42
186003909................................ 99
186003992.............................. 184
186004084...................... 188, 211
186004454.............................. 128
186004590................................ 44
186003910................................ 99
186003993.............................. 184
186004085...................... 188, 211
186004455.............................. 127
186004602................................ 49
186003911................................ 99
186003994.............................. 184
186004086...................... 188, 211
186004456.............................. 127
186004603................................ 49
186003915.......................... 34, 37
186003995.............................. 184
186004090.............................. 243
186004457.............................. 127
186004604................................ 49
186003925................................ 36
186003996.............................. 184
186004092.............................. 243
186004458.............................. 127
186004605................................ 49
186003928.............................. 186
186003997.............................. 184
186004095.............................. 243
186004459.............................. 127
186004606................................ 49
186003929.............................. 184
186003998.............................. 184
186004097.............................. 243
186004460.............................. 127
186004607................................ 49
186003930.............................. 184
186003999.............................. 184
186004099.............................. 243
186004461.............................. 127
186004608................................ 49
186003838.............................. 240
186003839.............................. 240
186003840.............................. 186
186003841.............................. 186
186003849................................ 36
186003852.......................... 34, 36
186003853.............................. 186
186003854.............................. 186
186003855.............................. 186
186003863.............................. 263
186003864.............................. 263
186003873........................ 80, 105
186003885C .................. 70, 73, 74
186003886C ................. 64, 68, 70,
.................................... 73, 74, 103
186003889.............................. 184
186003890.............................. 184
186003891.............................. 184
186003892.............................. 184
186003893.............................. 184
186003894.............................. 184
186003895.............................. 184
186003896.............................. 184
186003897.............................. 184
186003898.............................. 184
344
Part Number Index
www.waters.com
PaRT NUMbER INdEx

186004609................................ 49
186004690................................ 99
186004757.............................. 139
186004830................................ 55
186004927.............................. 197
186004610................................ 49
186004691.............................. 102
186004758.............................. 139
186004831................................ 55
186004928.............................. 197
186004611................................ 49
186004692.............................. 102
186004759.............................. 139
186004832................................ 55
186004929.............................. 224
186004612................................ 49
186004693.............................. 102
186004760.............................. 139
186004833................................ 55
186004930.............................. 224
186004613................................ 49
186004694.............................. 102
186004761.............................. 139
186004834................................ 55
186004931.............................. 224
186004614................................ 49
186004695.............................. 102
186004762.............................. 139
186004835................................ 55
186004932................................ 52
186004615................................ 49
186004696.............................. 102
186004763.............................. 139
186004837................................ 55
186004935.............................. 197
186004616................................ 49
186004697.............................. 102
186004764.............................. 139
186004839.............................. 100
186004936.............................. 197
186004617................................ 49
186004698.............................. 102
186004765.............................. 139
186004848.............................. 100
186004937.............................. 197
186004618................................ 49
186004699.............................. 102
186004766.............................. 139
186004849.............................. 100
186004938.............................. 197
186004619................................ 49
186004700.............................. 102
186004767.............................. 139
186004850.............................. 100
186004939.............................. 197
186004620................................ 49
186004701.............................. 102
186004768.............................. 139
186004852.............................. 139
186004940.............................. 197
186004621................................ 49
186004702.............................. 102
186004769.............................. 139
186004857.............................. 127
186004941.............................. 197
186004623.............................. 222
186004703.............................. 102
186004770.............................. 139
186004858.............................. 127
186004942.............................. 197
186004629.............................. 207
186004704.............................. 102
186004771.............................. 139
186004859.............................. 127
186004943.............................. 197
186004630.............................. 262
186004705.............................. 102
186004772.............................. 139
186004860.............................. 127
186004944.............................. 197
186004632................................ 73
186004709.............................. 102
186004773.............................. 139
186004861.............................. 127
186004945.............................. 197
186004639.............................. 262
186004711.............................. 184
186004774.............................. 139
186004862.............................. 127
186004946.............................. 197
186004640.............................. 262
186004712.............................. 188
186004775.............................. 139
186004863.............................. 127
186004947.............................. 197
186004641.............................. 262
186004713.............................. 209
186004776.............................. 139
186004864.............................. 127
186004948.............................. 197
186004646.......................... 35, 37
186004719................................ 52
186004777.............................. 139
186004865.............................. 128
186004949.............................. 197
186004647.......................... 35, 37
186004720.............................. 184
186004778.............................. 139
186004866.............................. 128
186004950.............................. 197
186004648.......................... 34, 36
186004721.............................. 184
186004779.............................. 139
186004867.............................. 128
186004951.............................. 197
186004649.......................... 34, 37
186004722.............................. 184
186004780.............................. 139
186004868.............................. 128
186004952.............................. 197
186004650.......................... 34, 37
186004723.............................. 184
186004781.............................. 139
186004869.............................. 128
186004953.............................. 197
186004651.......................... 34, 37
186004724.............................. 184
186004782.............................. 139
186004870.............................. 128
186004954.............................. 197
186004659................................ 99
186004725.............................. 184
186004783.............................. 139
186004871.............................. 127
186004955.............................. 197
186004660................................ 99
186004726.............................. 184
186004784.............................. 139
186004896.............................. 207
186004958.............................. 102
186004661................................ 99
186004727.............................. 184
186004785.............................. 139
186004897.............................. 207
186004959.............................. 102
186004663................................ 99
186004728.............................. 184
186004786.............................. 139
186004898.............................. 246
186004960.............................. 102
186004664................................ 99
186004729.............................. 184
186004787.............................. 139
186004899.............................. 222
186004961.............................. 102
186004665................................ 99
186004730.............................. 184
186004788.............................. 139
186004900.............. 221, 222, 255
186004962.............................. 102
186004666................................ 99
186004731.............................. 184
186004789.............................. 139
186004904.............................. 207
186004963.............................. 102
186004667................................ 99
186004732.............................. 184
186004790.............................. 139
186004905.............................. 207
186004964.............................. 102
186004668................................ 99
186004733.............................. 184
186004791.............................. 139
186004906.............................. 246
186004965.............................. 102
186004669................................ 99
186004734.............................. 184
186004792.............................. 139
186004907.............................. 251
186004966.............................. 102
186004670................................ 99
186004735.............................. 184
186004793.............................. 139
186004908.............................. 197
186004967.............................. 102
186004671................................ 99
186004739.............................. 251
186004794.............................. 139
186004909.............................. 197
186004968.............................. 102
186004672................................ 99
186004740.............................. 251
186004795.............................. 139
186004910.............................. 197
186004969.............................. 102
186004673................................ 99
186004741.............................. 251
186004796.............................. 139
186004911.............................. 197
186004970.............................. 102
186004674................................ 99
186004742.............................. 251
186004797.............................. 139
186004912.............................. 197
186004971.............................. 102
186004675.............................. 100
186004743.............................. 139
186004798.............................. 139
186004913.............................. 197
186004972.............................. 102
186004676.............................. 100
186004744.............................. 139
186004799.............................. 102
186004914.............................. 197
186004973.............................. 102
186004677.............................. 100
186004745.............................. 139
186004800.............................. 100
186004915.............................. 197
186004974.............................. 102
186004678.............................. 100
186004746.............................. 139
186004801.............................. 100
186004916.............................. 197
186004975.............................. 102
186004679...................... 100, 211
186004747.............................. 139
186004802.............................. 100
186004917.............................. 197
186004976.............................. 102
186004680...................... 100, 211
186004748.............................. 139
186004803.............................. 100
186004918.............................. 197
186004977.............................. 102
186004681...................... 100, 211
186004749.............................. 139
186004804.............................. 100
186004919.............................. 197
186005219.......................... 70, 74
186004682.............................. 100
186004750.............................. 139
186004805.............................. 100
186004920.............................. 197
186005220.......................... 70, 74
186004683...................... 100, 211
186004751.............................. 139
186004806.............................. 100
186004921.............................. 197
186005221............ 64, 70, 74, 103
186004684...................... 100, 211
186004752.............................. 139
186004807.............................. 102
186004922.............................. 197
186005222.......................... 71, 75
186004685...................... 100, 211
186004753.............................. 139
186004808.............................. 102
186004923.............................. 197
186005223.......................... 71, 75
186004686.............................. 100
186004754.............................. 139
186004809.............................. 102
186004924.............................. 197
186005224.......................... 71, 75
186004687...................... 100, 211
186004755.............................. 139
186004810.............................. 102
186004925.............................. 197
186005225.............................. 220
186004689...................... 100, 211
186004756.............................. 139
186004826...................... 100, 211
186004926.............................. 197
186005226.............................. 220
www.waters.com
Part Number Index
345
PaRT NUMbER INdEx

186005234.................... 38, 50, 51
186005303.............................. 102
186005358.............................. 102
186005413.............................. 102
186005510.............................. 182
186005249.............................. 121
186005304.............................. 121
186005359.............................. 121
186005414.............................. 182
186005511.............................. 182
186005250.............................. 121
186005305.............................. 121
186005360.............................. 121
186005415.............................. 182
186005516.............................. 246
186005251.............................. 121
186005306.............................. 121
186005361.............................. 121
186005416.............................. 182
186005518........................ 53, 105
186005252.............................. 121
186005307.............................. 121
186005362.............................. 121
186005417.............................. 182
186005520.............................. 182
186005253.............................. 121
186005308.............................. 121
186005363.............................. 121
186005418.............................. 182
186005521.......................... 38, 51
186005254.............................. 121
186005309.............................. 121
186005364.............................. 121
186005420.............................. 182
186005522.......................... 38, 51
186005255.............................. 121
186005310.............................. 121
186005365.............................. 121
186005421.............................. 182
186005523.......................... 38, 51
186005256.............................. 121
186005311.............................. 121
186005366.............................. 121
186005422.............................. 182
186005524.......................... 38, 51
186005257.............................. 121
186005312.............................. 121
186005367.............................. 121
186005423.............................. 182
186005529.............................. 102
186005258.............................. 121
186005313.............................. 121
186005368.............................. 121
186005424.............................. 182
186005530.............................. 102
186005259.............................. 121
186005314.............................. 121
186005369.............................. 121
186005425.............................. 182
186005531.............................. 102
186005260.............................. 121
186005315.............................. 121
186005370.............................. 121
186005426.............................. 182
186005533.............................. 102
186005261.............................. 121
186005316.............................. 121
186005371.............................. 121
186005427.............................. 182
186005534.............................. 102
186005262.............................. 121
186005317.............................. 121
186005372.............................. 121
186005428.............................. 182
186005535.............................. 102
186005263.............................. 121
186005318.............................. 121
186005373.............................. 121
186005429.............................. 182
186005536.............................. 102
186005264.............................. 121
186005319.............................. 121
186005374.............................. 121
186005430.............................. 182
186005537.............................. 102
186005265.............................. 121
186005320.............................. 121
186005375.............................. 121
186005431.............................. 182
186005538.............................. 121
186005266.............................. 121
186005321.............................. 121
186005376.............................. 121
186005433.............................. 182
186005539.............................. 121
186005267.............................. 121
186005322.............................. 121
186005377.............................. 121
186005434.............................. 182
186005540.............................. 121
186005268.............................. 121
186005323.............................. 121
186005378.............................. 121
186005435.............................. 182
186005541.............................. 121
186005269.............................. 121
186005324.............................. 121
186005379.............................. 121
186005436.............................. 182
186005542.............................. 121
186005270.............................. 121
186005325.............................. 121
186005380.............................. 121
186005437.............................. 182
186005543.............................. 121
186005271.............................. 121
186005326.............................. 121
186005381.............................. 121
186005438.............................. 182
186005544.............................. 121
186005272.............................. 121
186005327.............................. 121
186005382.............................. 121
186005439.............................. 182
186005545.............................. 121
186005273.............................. 121
186005328.............................. 121
186005383.............................. 121
186005440.............................. 182
186005546.............................. 121
186005274.............................. 121
186005329.............................. 121
186005384.............................. 121
186005441.............................. 182
186005547.............................. 121
186005275.............................. 121
186005330.............................. 121
186005385.............................. 121
186005442.............................. 182
186005548.............................. 121
186005276.............................. 121
186005331.............................. 121
186005386.............................. 121
186005443.............................. 182
186005549.............................. 121
186005277.............................. 121
186005332.............................. 121
186005387.............................. 121
186005444.............................. 182
186005550.............................. 121
186005278.............................. 121
186005333.............................. 121
186005388.............................. 121
186005446.............................. 182
186005551.............................. 121
186005279.............................. 121
186005334.............................. 121
186005389.............................. 121
186005447.............................. 182
186005552.............................. 121
186005280.............................. 121
186005335.............................. 121
186005390.............................. 121
186005448.............................. 182
186005553.............................. 121
186005281.............................. 121
186005336.............................. 121
186005391.............................. 121
186005450.............................. 182
186005554.............................. 121
186005282.............................. 121
186005337.............................. 121
186005392.............................. 121
186005451.............................. 182
186005555.............................. 121
186005283.............................. 121
186005338.............................. 121
186005393.............................. 121
186005452.............................. 182
186005556.............................. 121
186005284.............................. 121
186005339.............................. 121
186005394.............................. 121
186005491.............................. 182
186005557.............................. 121
186005285.............................. 121
186005340.............................. 121
186005395.............................. 121
186005492.............................. 182
186005558.............................. 121
186005286.............................. 121
186005341.............................. 121
186005396.............................. 121
186005493.............................. 182
186005559.............................. 121
186005287.............................. 121
186005342.............................. 121
186005397.............................. 121
186005494.............................. 182
186005560.............................. 121
186005288.............................. 121
186005343.............................. 121
186005398.............................. 121
186005495.............................. 182
186005561.............................. 121
186005289.............................. 121
186005344.............................. 121
186005399.............................. 121
186005496.............................. 182
186005562.............................. 121
186005290.............................. 121
186005345.............................. 121
186005400.............................. 121
186005497.............................. 182
186005563.............................. 121
186005291.............................. 121
186005346.............................. 121
186005401.............................. 121
186005498.............................. 182
186005564.............................. 121
186005292................................ 99
186005347................................ 99
186005402................................ 99
186005499.............................. 182
186005565.............................. 121
186005293................................ 99
186005348................................ 99
186005403................................ 99
186005500.............................. 182
186005566.............................. 121
186005294................................ 99
186005349................................ 99
186005404................................ 99
186005501.............................. 182
186005567.............................. 121
186005295................................ 99
186005350................................ 99
186005405................................ 99
186005502.............................. 182
186005568.............................. 121
186005296................................ 99
186005351................................ 99
186005406................................ 99
186005503.............................. 182
186005569.............................. 121
186005297................................ 99
186005352................................ 99
186005407................................ 99
186005504.............................. 182
186005570.............................. 121
186005298................................ 99
186005353................................ 99
186005408................................ 99
186005505.............................. 182
186005571.............................. 102
186005299................................ 99
186005354................................ 99
186005409................................ 99
186005506.............................. 182
186005572.............................. 102
186005300................................ 99
186005355................................ 99
186005410................................ 99
186005507.............................. 182
186005573.............................. 102
186005301................................ 99
186005356................................ 99
186005411................................ 99
186005508.............................. 182
186005574.............................. 102
186005302................................ 99
186005357................................ 99
186005412................................ 99
186005509.............................. 182
186005575.............................. 102
346
Part Number Index
www.waters.com
PaRT NUMbER INdEx

186005576.............................. 102
201000144.............................. 317
205000115.............................. 303
205015015.............................. 291
430000782.............................. 293
186005577.............................. 102
201000145.............................. 318
205000116.............................. 303
205015016.............................. 286
430000783.............................. 293
186005578.............................. 102
201000146.............................. 318
205000117.............................. 303
205015017...................... 286, 287
430000784.............................. 293
186005579.............................. 102
201000147.............................. 318
205000118.............................. 303
205015019...................... 286, 287
430000785.............................. 293
186005580.............................. 102
201000149.............. 313, 314, 317
205000119.............................. 303
205016040.............................. 291
430001017.............................. 289
186005581.............................. 102
201000150.............................. 318
205000134.............................. 303
289000439.............................. 291
430001020.............................. 285
186005582.............................. 102
201000151.............................. 318
205000159.............................. 291
289000440.............................. 304
430001084.............................. 286
186005586.......................... 38, 51
201000152.............................. 318
205000202.............................. 295
289000614.............................. 306
430001086.............................. 286
186005587.............. 36, 38, 47, 50
201000153.............................. 318
205000250.............................. 295
430001113...................... 285, 290
186005589.............................. 222
201000155.............................. 318
205000251.............................. 295
289000779............. 184, 186, 188,

................................ 190, 208, 211
186005590.............................. 222
201000159...... 285, 286, 308, 309
205000252.............................. 295
289000935.............................. 306
201000161.............................. 297
205000263.............................. 286
289001680.............................. 290
430001115.............................. 290
186005591.............................. 222
186005592.............................. 207
201000162.............................. 305
205000275.............................. 289
289002111.............................. 290
186005593.............................. 207
201000169.............................. 293
205000291...................... 285, 286
289002172.............................. 290
186005594.............................. 207
201000170.............................. 313
205000321.............................. 306
289002414...................... 288, 290
201000173.............................. 285
205000322.............................. 306
289002576.............................. 291
201000174...................... 285, 286
205000323.............................. 306
289002802.............................. 291
201000178.............................. 317
205000324.............................. 297
289003345.............................. 291
201000181.............................. 290
205000342.............................. 286
289004407.............................. 291
201000182...................... 290, 291
205000343..................... 243, 102,

........................................ 243, 207
289004442.............................. 291
186005604................................ 99
186005605................................ 99
186005606................................ 99
186005607................................ 99
186005608.............................. 100
186005609................................ 99
186005610................................ 99
186005611................................ 99
186005612................................ 99
186005613.............................. 100
186005614.............................. 100
186005615.............................. 100
186005616.............................. 100
186005617.............................. 100
186005618.............................. 100
186005619.............................. 100
186005620................................ 99
186005621................................ 99
186005622................................ 99
186005623................................ 99
186005624................................ 99
186005625................................ 99
186005657.............................. 100
186005658.............................. 100
201000102.............................. 301
430001114.............................. 290
430001116.............................. 290
430001120.............................. 290
430001121...................... 285, 290
430001207.............................. 285
430001209.............................. 290
430001210.............................. 290
430001221.............................. 286
430001229.............................. 289
430001264.............. 243, 286, 291
205000362.............................. 286
323000247............. 286, 287, 291,

........................................ 306, 307
201000187.............................. 311
205000363.............................. 286
323000446...................... 287, 307
201000188.............................. 318
205000365...................... 285, 286
405000934................................ 36
201000189.............................. 318
205000369.............................. 286
405003015.............................. 288
201000193.............................. 285
205000370.............................. 286
405003740.............. 286, 287, 291
201000195.............................. 304
205000398.............................. 291
405003741.............. 286, 287, 291
201000196.............................. 302
205000399.............................. 307
405003742.............. 286, 287, 291
201000197.............................. 285
205000400.............................. 307
405003743................ 64, 286, 103
201000198.............................. 289
205000401.............................. 307
405005067.............................. 291
201000199.............................. 299
205000402.............................. 307
405005068.............................. 291
201000203.............................. 312
205000403.............................. 285
405006414.............................. 299
201000204.............................. 312
205000404.............................. 285
405006528.......................... 38, 51
201000205.............................. 312
205000478...................... 285, 288
405007627.............................. 288
201000206.............................. 312
205000494.............................. 243
405008440.............................. 299
201000208.............................. 287
205000505.............. 287, 298, 299
405008854.............................. 288
201000209.............................. 298
205000540.............................. 291
405010508.............................. 288
201000210.............................. 299
205000542.............................. 291
405011492.............................. 288
201000219.............................. 311
205000585.............................. 289
410000570...................... 298, 299
430001565.............................. 286
201000224.............................. 311
205000589.............................. 290
410001296.............................. 288
430001568.............................. 290
201000186............. 285, 290, 306,

........................................ 307, 308
430001311.............. 286, 289, 291

430001320...................... 286, 291
430001325...................... 286, 291
430001326.............. 286, 289, 291
430001346.............................. 290
430001389.............................. 290
430001436.............................. 290
430001443.............................. 285
430001456.............................. 291
430001470.............................. 291
430001486.............................. 285
430001511.............................. 290
430001512.............................. 290
430001534.............................. 290
430001535.............................. 290
430001556.............................. 307
430001562.............................. 286
201000229.............................. 311
205000590.............................. 289
410001347.............................. 286
430001569.............................. 290
201000107.............................. 293
201000230.............................. 311
205000650.............................. 288
410001348.............................. 291
430001570.............................. 291
201000111.............................. 310
201000233.............................. 288
205000726.............................. 288
410001659.............................. 286
430001571...................... 262, 291
201000113.............................. 296
201000234.............................. 288
205000730.............................. 288
410001697.............................. 291
430001572.............................. 290
201000114.............................. 297
201000235.............................. 315
205004100.............................. 309
410001905.............................. 285
430001575...................... 262, 291
201000116.............................. 305
201000236.............................. 315
205004115.............................. 309
410002096.............................. 287
430001576.............................. 262
201000117.............................. 305
201000238.............................. 312
205004215.............................. 309
410003180.............................. 288
430001577...................... 262, 291
201000118.............................. 305
201000239.............................. 312
205004220.............................. 309
410003295.............................. 288
430001629...................... 262, 291
201000119.............................. 305
201000240.............................. 312
205004325.............................. 309
415000415.............................. 309
430001748.............................. 286
201000120.............................. 305
201000241.............................. 312
205004330.............................. 309
415001544.............................. 288
430001749.............................. 286
201000121.............................. 305
205000105.............................. 303
205015000.............................. 286
415001608.............................. 288
430001773.............................. 285
201000122.............................. 305
205000106.............................. 303
205015001.............................. 286
425000326.............................. 286
430001783...................... 286, 287
201000125.............................. 305
205000107.............................. 303
205015002.............................. 286
425000536.............................. 286
430001784...................... 286, 287
201000130.............................. 298
205000108.............................. 303
205015003.............................. 286
430000366.............................. 323
430001835.............................. 291
201000131...................... 308, 310
205000109.............................. 303
205015009.............................. 286
430000762.............................. 293
430001837.............................. 291
201000132.............................. 303
205000112.............................. 303
205015013.............................. 291
430000781.............................. 293
430001848.............................. 291
www.waters.com
Part Number Index
347
PaRT NUMbER INdEx

430002015.............................. 289
700000146.............................. 301
700001951.............................. 309
700002676.............. 286, 287, 291
700003928.............................. 302
430002032...................... 298, 299
700000168.............................. 306
700001952.............................. 309
700002682.............................. 286
700004023.............................. 288
430002121...................... 298, 299
700000169.............................. 306
700001953.............................. 309
700002696.............................. 290
700004057.............................. 289
430002140...................... 262, 291
700000201.............................. 292
700001954.............................. 309
700002702.............................. 290
700004063.............................. 302
430002153...................... 262, 291
700000253.............. 295, 296, 300
700001955.............................. 309
700002705.............................. 289
700004086.............................. 302
430002155...................... 262, 291
700000254.............. 295, 296, 297
700001956.............................. 309
700002708.............................. 291
700004088.............................. 302
430002183...................... 262, 291
700001957.............................. 309
700002712.............................. 290
700004089.............................. 302
430002208.............................. 288
700000338..................... 313, 314,

........................................ 316, 317
700001958.............................. 309
700002713.............................. 290
700004090.............................. 302
430002242.............................. 291
700000383.............................. 295
700001959.............................. 309
700002747.............................. 291
700004144.............................. 289
430002316.............................. 288
700000384.............................. 295
700001960.............................. 309
700002754.............................. 291
700004145.............................. 289
430002317.............................. 288
700000385.............................. 295
700001961.............................. 309
700002755.............................. 291
700004147.............................. 289
430002319.............................. 288
700000389.............................. 295
700001962.............................. 309
700002757.............................. 291
700004155.............................. 306
430002357.............................. 288
700000471.............................. 304
700001963.............................. 309
700002761.............................. 295
700004425...................... 298, 299
430002387.............................. 288
700000564.............................. 295
700001964.............................. 309
700002765.............................. 286
700004430...................... 298, 299
430002388.............................. 288
700000565.............................. 295
700001965.............................. 309
700002775...................... 207, 102
700004431...................... 298, 299
430002389.............................. 288
700000574.............................. 309
700001968.............................. 309
700002791.............................. 295
700004436.............................. 299
430002390.............................. 288
700000610.............................. 291
700001985.............................. 309
700002801.............................. 286
700004439...................... 298, 299
430002400.............................. 288
700000611.............................. 295
700001994.............................. 309
700002907.............................. 291
700004445...................... 298, 299
440000145.............................. 286
700000680.............................. 302
700002003.............................. 309
700002908.............................. 291
700004454...................... 298, 299
441000372.............. 287, 306, 307
700000683.............................. 302
700002069.............................. 309
700002911.............................. 285
700004599.............................. 291
441000373...................... 287, 307
700000684.............................. 302
700002103.............................. 309
700002912.............................. 285
700004601.............................. 291
441000456...................... 286, 287
700000685.............................. 302
700002964.............................. 285
700004607.............................. 299
441001040.............................. 288
700000850.............................. 310
700002210.............................. 305
700002965.............................. 285
700004613...................... 298, 299
600000138.............................. 310
700000923.............................. 306
700002275.............................. 295
700002968...................... 285, 290
700004673.............................. 315
600000162................................ 76
700001004.............................. 309
700002282.............................. 295
700003114...................... 285, 286
700004715.............................. 228
600000175.............................. 320
700001012...................... 285, 286
700002292.............................. 305
700003115.............................. 286
700004730.............................. 315
600000176.............................. 320
700001019............. 184, 186, 188,

................................ 190, 208, 211
700002293.............................. 305
700002297.............................. 305
700003116.............................. 286
700004790.............................. 102
700002298.............................. 305
700003139.............................. 285
700004791.............................. 102
700002332.............................. 295
700003147.............................. 285
700004792.............................. 102
700002333.............................. 295
700003151.............................. 285
700005083.............................. 298
700002399.............................. 295
700003156.............................. 285
700005084.............................. 299
700002439.............................. 305
700003164.............................. 285
700005119.............................. 288
700002455.............................. 289
700003167.............................. 285
700005162.............................. 288
700002569...................... 286, 291
700003168.............................. 285
700005164.............................. 288
700002570.............. 286, 288, 291
700003169.............................. 285
700005165.............................. 288
700002572...................... 286, 291
700003170.............................. 285
700005167.............................. 288
700002576.............................. 291
700003423...................... 306, 307
700005173.............................. 288
700001493.............................. 298
700002577.............................. 291
700003557.............................. 285
700005215.............................. 288
700001494.............................. 298
700002587.............................. 286
700003615.............................. 285
700005234.............................. 288
700001502.............................. 290
700002594.............. 285, 288, 290
700003616.............................. 285
700005236.............................. 288
700001529...................... 298, 299
700002595.............. 285, 288, 290
700003694.............................. 287
700005248.............................. 288
600000670CV ............... 64, 68, 70,

.................................... 73, 74, 103
700001530.............. 288, 298, 299
700002596...................... 285, 290
700003711.............................. 287
700005251.............................. 288
700001618.............................. 310
700002597...................... 285, 288
700003775.............................. 102
700005269.............................. 287
600000671CV ...................... 68, 70
700001710.............................. 309
700002598.............. 285, 288, 290
700003776.............................. 102
715001357.............................. 262
600000749CV ...................... 70, 74
700001786.............................. 306
700002599.............. 285, 288, 290
700003777.............................. 102
715001531.......................... 6, 161
600000750CV ............................ 70
700001796...................... 307, 308
700002600.............. 285, 288, 290
700003778.............................. 102
715001940.................................. 6
600000751CV ...................... 70, 74
700002601.............. 285, 288, 290
700003779.............................. 102
715002098.......................... 6, 100
600000752CV ...................... 70, 74
700001800............. 286, 287, 291,

........................................ 306, 307
700002603...................... 285, 290
700003780.............................. 102
715002099.......................... 6, 100
600000754CV ...................... 70, 74
700001836.............................. 299
700002604...................... 285, 290
700003794.............................. 287
715002531.......................... 100, 6
600000755CV ............... 64, 68, 70,

.................................... 73, 74, 103
700001840.............................. 310
700002634.............. 285, 289, 291
700003872.............................. 302
725000106.............................. 304
700001942.............................. 309
700002635...... 285, 288, 289, 290
700003921.............................. 302
725000107.............................. 304
600001043.............................. 209
700001943.............................. 309
700002636.............................. 285
700003922.............................. 302
725000110.............................. 304
605000101.............................. 286
700001948.............................. 309
700002644.............................. 286
700003923.............................. 302
725000113.............................. 304
700000117.............................. 165
700001949.............................. 309
700002645.............. 285, 289, 290
700003924.............................. 302
725000144.............................. 304
700000145.............................. 301
700001950.............................. 309
700002660.............. 285, 288, 290
700003925.............................. 302
725000145.............................. 304
600000177.............................. 320
600000178.............................. 320
600000179.............................. 320
600000180.............................. 320
600000181.............................. 320
600000182.............................. 320
600000183.............................. 320
600000184.............................. 320
600000227.............................. 310
600000228.............................. 310
600000229.............................. 310
600000668CV ............... 64, 68, 70,
.................................... 73, 74, 103
600000669CV ............... 64, 68, 70,
.................................... 73, 74, 103
348
700001020............. 184, 186, 188,

................................ 190, 208, 211
700001183.............................. 309
700001218.............................. 295
700001230.............................. 305
700001247.............................. 295
700001326.............................. 295
700001397.............................. 303
700001436............. 184, 186, 188,
................................ 190, 208, 211
Part Number Index
www.waters.com
PaRT NUMbER INdEx

725000146.............................. 304
PSS614102 ...................... 154, 320
PSS830815 .............................. 153
PSS832011 .............................. 153
PSS832911 .............................. 153
725000147.............................. 304
PSS614103 ...................... 154, 320
PSS830885 .............................. 153
PSS832012 .............................. 153
PSS832912 .............................. 153
725000152.............................. 304
PSS614104 ...................... 154, 320
PSS830895 .............................. 153
PSS832013 .............................. 153
PSS832913 .............................. 153
725000417.................. 209, 38, 38
PSS614108 ...................... 154, 320
PSS830908 .............................. 153
PSS832022 .............................. 154
PSS833013 .............................. 152
725000418.................. 209, 38, 38
PSS614113 ...................... 154, 320
PSS830909 .............................. 153
PSS832023 .............................. 154
PSS833015 .............................. 152
725000455.............................. 291
PSS830008 ...................... 154, 320
PSS830911 .............................. 153
PSS832032 .............................. 154
PSS833085 ...................... 153, 191
725000464...................... 288, 289
PSS830051 .............................. 154
PSS830912 .............................. 153
PSS832033 .............................. 154
PSS833095 ...................... 153, 191
JJAN20131 ................................. 44
PSS830053 .............................. 154
PSS830913 .............................. 153
PSS832111 .............................. 153
PSS833111 .............................. 153
JJAN20229 ................................. 44
PSS830055 .............................. 154
PSS830915 .............................. 153
PSS832112 .............................. 153
PSS833112 .............................. 153
M956335DC2 ........... 313, 314, 317
PSS830057 .............................. 154
PSS830922 .............................. 154
PSS832113 .............................. 153
PSS833113 .............................. 153
PSL404219........................... 71, 75
PSS830059 .............................. 154
PSS830923 .............................. 154
PSS832122 .............................. 154
PSS833213 .............................. 152
PSL404231........................... 71, 75
PSS830065 .............................. 153
PSS830925 .............................. 154
PSS832123 .............................. 154
PSS833215 .............................. 152
PSL613301............................... 322
PSS830068 .............................. 153
PSS830932 .............................. 154
PSS832132 .............................. 154
PSS833285 ...................... 153, 191
PSL613302............................... 322
PSS830073 .............................. 154
PSS830985 .............................. 153
PSS832133 .............................. 154
PSS833295 ...................... 153, 191
PSL613312............................... 324
PSS830074 .............................. 154
PSS830995 .............................. 153
PSS832211 .............................. 153
PSS833411 .............................. 153
PSL613313............................... 324
PSS830075 .............................. 154
PSS831011 .............................. 153
PSS832212 .............................. 153
PSS833412 .............................. 153
PSL613315....................... 322, 324
PSS830076 .............................. 154
PSS831012 .............................. 153
PSS832213 .............................. 153
PSS833413 .............................. 153
PSL613316............................... 322
PSS830077 .............................. 154
PSS831013 .............................. 153
PSS832222 .............................. 154
PSS833422 .............................. 154
PSL613317............................... 324
PSS830078 .............................. 154
PSS831015 .............................. 153
PSS832223 .............................. 154
PSS833423 .............................. 154
PSL613318............................... 324
PSS830079 .............................. 154
PSS831032 .............................. 154
PSS832232 .............................. 154
PSS833433 .............................. 154
PSL613319............................... 324
PSS830111 .............................. 153
PSS831085 .............................. 153
PSS832233 .............................. 154
PSS833512 .............................. 152
PSL613320............................... 324
PSS830112 .............................. 153
PSS831095 .............................. 153
PSS832311 .............................. 153
PSS833513 .............................. 152
PSL613321............................... 324
PSS830113 .............................. 153
PSS831111 .............................. 153
PSS832312 .............................. 153
PSS833515 .............................. 152
PSL613322............................... 322
PSS830115 .............................. 153
PSS831112 .............................. 153
PSS832313 .............................. 153
PSS833585 ...................... 153, 191
PSL613324............................... 322
PSS830122 .............................. 154
PSS831113 .............................. 153
PSS832322 .............................. 154
PSS833595 ...................... 153, 191
PSL613325............................... 322
PSS830123 .............................. 154
PSS831115 .............................. 153
PSS832323 .............................. 154
PSS833612 .............................. 152
PSL613457............................... 325
PSS830125 .............................. 154
PSS831122 .............................. 154
PSS832333 .............................. 154
PSS833613 .............................. 152
PSL613570............................... 325
PSS830135 .............................. 154
PSS831123 .............................. 154
PSS832411 .............................. 153
PSS833615 .............................. 152
PSL613578............................... 325
PSS830185 .............................. 153
PSS831125 .............................. 154
PSS832412 .............................. 153
PSS833685 ...................... 153, 191
PSL613600............................... 325
PSS830195 .............................. 153
PSS831132 .............................. 154
PSS832413 .............................. 153
PSS833695 ...................... 153, 191
PSL613602............................... 325
PSS830212 .............................. 152
PSS831135 .............................. 154
PSS832422 .............................. 154
PSS833711 .............................. 153
PSL613604............................... 325
PSS830213 .............................. 152
PSS831185 .............................. 153
PSS832423 .............................. 154
PSS833712 .............................. 153
PSL613607............................... 325
PSS830214 .............................. 152
PSS831195 .............................. 153
PSS832432 .............................. 154
PSS833713 .............................. 153
PSL613608............................... 325
PSS830215 .............................. 152
PSS831811 .............................. 153
PSS832433 .............................. 154
PSS833813 .............................. 152
PSL613609............................... 325
PSS830285 ...................... 153, 191
PSS831812 .............................. 153
PSS832513 .............................. 152
PSS833815 .............................. 152
PSL618000............................... 321
PSS830295 ...................... 153, 191
PSS831813 .............................. 153
PSS832514 .............................. 152
PSS833885 ...................... 153, 191
PSL618001............................... 321
PSS830611 .............................. 153
PSS831815 .............................. 153
PSS832515 .............................. 152
PSS833895 ...................... 153, 191
PSL618003............................... 321
PSS830612 .............................. 153
PSS831822 .............................. 154
PSS832585 ...................... 153, 191
PSS833912 .............................. 152
PSL618004............................... 321
PSS830613 .............................. 153
PSS831823 .............................. 154
PSS832595 ...................... 153, 191
PSS833913 .............................. 152
PSL618005............................... 321
PSS830615 .............................. 153
PSS831825 .............................. 154
PSS832611 .............................. 153
PSS833915 .............................. 152
PSL618006............................... 321
PSS830622 .............................. 154
PSS831832 .............................. 154
PSS832612 .............................. 153
PSS833985 ...................... 153, 191
PSL618007............................... 321
PSS830623 .............................. 154
PSS831885 .............................. 153
PSS832613 .............................. 153
PSS833995 ...................... 153, 191
PSL618008............................... 321
PSS830625 .............................. 154
PSS831895 .............................. 153
PSS832615 .............................. 153
PSS837511 .............................. 153
PSL618009............................... 321
PSS830632 .............................. 154
PSS831911 .............................. 153
PSS832685 .............................. 153
PSS837512 .............................. 153
PSL618010............................... 321
PSS830635 .............................. 154
PSS831912 .............................. 153
PSS832695 .............................. 153
PSS837513 .............................. 153
PSL618011............................... 321
PSS830685 .............................. 153
PSS831913 .............................. 153
PSS832711 .............................. 153
PSS837515 .............................. 153
PSL618021............................... 324
PSS830695 .............................. 153
PSS831915 .............................. 153
PSS832712 .............................. 153
PSS837585 .............................. 153
PSL618022............................... 324
PSS830712 .............................. 152
PSS831922 .............................. 154
PSS832713 .............................. 153
PSS837595 .............................. 153
PSL901282............................... 325
PSS830713 .............................. 152
PSS831923 .............................. 154
PSS832715 .............................. 153
PSS837613 .............................. 152
PSL901290............................... 323
PSS830714 .............................. 152
PSS831925 .............................. 154
PSS832785 .............................. 153
PSS837615 .............................. 152
PSL901291............................... 323
PSS830715 .............................. 152
PSS831932 .............................. 154
PSS832795 .............................. 153
PSS837685 ...................... 153, 191
PSL901292............................... 323
PSS830785 ...................... 153, 191
PSS831933 .............................. 154
PSS832813 .............................. 152
PSS837695 ...................... 153, 191
PSL901294............................... 323
PSS830795 ...................... 153, 191
PSS831935 .............................. 154
PSS832815 .............................. 152
PSS837812 .............................. 153
PSS614100 ...................... 154, 320
PSS830812 .............................. 153
PSS831985 .............................. 153
PSS832885 ...................... 153, 191
PSS837813 .............................. 153
PSS614101 ...................... 154, 320
PSS830813 .............................. 153
PSS831995 .............................. 153
PSS832895 ...................... 153, 191
PSS837815 .............................. 153
www.waters.com
Part Number Index
349
PaRT NUMbER INdEx

PSS838521 .............................. 153
PSS839541 .............................. 153
PSS845300 .............................. 153
WAT005138 .............................. 229
WAT011545 ...................... 170, 234
PSS838522 .............................. 153
PSS839542 .............................. 153
PSS845301 .............................. 153
WAT005139 .......................... 36, 38
WAT011550 ...................... 170, 234
PSS838523 .............................. 153
PSS839543 .............................. 153
PSS845304 .............................. 153
WAT005147 .............................. 195
WAT011565 ...................... 170, 234
PSS838524 .............................. 153
PSS839546 .............................. 153
PSS845305 .............................. 153
WAT007020 .............................. 323
WAT011570 ...................... 170, 234
PSS838525 .............................. 153
PSS839547 .............................. 153
PSS845308 .............................. 153
WAT007272 .............................. 295
WAT011572 .............................. 280
PSS838526 .............................. 153
PSS839548 .............................. 153
PSS845309 .............................. 153
WAT007354 .............................. 175
WAT011574 .............................. 280
PSS838529 .............................. 153
PSS839551 .............................. 153
PSS845539 .............................. 153
WAT007355 .............................. 174
WAT011576 .............................. 280
PSS838530 .............................. 153
PSS839552 .............................. 153
PSS845540 .............................. 153
WAT007356 .............................. 175
WAT011578 .............................. 280
PSS838531 .............................. 153
PSS839553 .............................. 153
PSS845541 .............................. 153
WAT007357 .............................. 174
WAT011580 .............................. 280
PSS838533 .............................. 153
PSS839556 .............................. 153
PSS845542 .............................. 153
WAT007358 .............................. 174
WAT011582 .............................. 280
PSS838534 .............................. 153
PSS839557 .............................. 153
PSS845543 .............................. 153
WAT007360 .............................. 242
WAT011584 .............................. 280
PSS838535 .............................. 153
PSS839558 .............................. 153
PSS845549 .............................. 153
WAT007571 .............................. 240
WAT011586 .............................. 280
PSS839451 .............................. 154
PSS839561 .............................. 153
PSS845550 .............................. 153
WAT010001 .............................. 192
WAT011588 .............................. 280
PSS839458 .............................. 154
PSS839562 .............................. 153
PSS845551 .............................. 153
WAT011590 .............................. 280
PSS839465 .............................. 154
PSS839563 .............................. 153
PSS845552 .............................. 153
WAT010004 .............................. 192

WAT010025 .............................. 156
WAT011592 .............................. 280
PSS839470 .............................. 154
PSS839566 .............................. 153
WAT010223 .............................. 193
WAT011594 .............................. 280
PSS839471 .............................. 154
PSS839567 .............................. 153
WAT010224 .............................. 193
WAT011596 .............................. 280
PSS839472 .............................. 154
PSS839568 .............................. 153
WAT010270 .............................. 156
WAT011598 .............................. 280
PSS839473 .............................. 154
PSS839570 .............................. 153
WAT010290 ...................... 173, 175
WAT011600 .............................. 280
PSS839474 .............................. 154
PSS839571 .............................. 153
WAT010295 .............................. 175
WAT011602 .............................. 280
PSS839475 .............................. 154
PSS839572 .............................. 153
WAT010551 .............................. 174
WAT011604 .............................. 280
PSS839476 .............................. 154
PSS839573 .............................. 153
WAT010565 .............................. 175
WAT011606 .............................. 280
PSS839477 .............................. 154
PSS839575 .............................. 153
WAT010570 .............................. 173
WAT011608 .............................. 280
PSS839478 .............................. 154
PSS839576 .............................. 153
WAT010571 .............................. 173
WAT011610 .............................. 280
PSS839502 .............................. 153
PSS839577 .............................. 153
WAT010639 .............................. 173
WAT011612 .............................. 280
PSS839503 .............................. 153
PSS839578 .............................. 153
WAT010640 .............................. 227
WAT011614 .............................. 280
PSS839505 .............................. 153
PSS839581 .............................. 153
WAT010656 .............................. 156
WAT011616 .............................. 280
PSS839506 .............................. 153
PSS839582 .............................. 153
WAT010657 .............................. 193
WAT011618 .............................. 280
PSS839507 .............................. 153
PSS839583 .............................. 153
WAT010658 .............................. 193
WAT011620 .............................. 280
PSS839508 .............................. 153
PSS839587 .............................. 153
WAT010739 .............................. 230
WAT011622 .............................. 280
PSS839510 .............................. 153
PSS839588 .............................. 153
WAT010740 .............................. 230
WAT011624 .............................. 280
PSS839511 .............................. 153
PSS839591 .............................. 153
WAT010741 .............................. 230
WAT011626 .............................. 280
PSS839512 .............................. 153
PSS839592 .............................. 153
WAT010742 .............................. 230
WAT011628 .............................. 280
PSS839513 .............................. 153
PSS839593 .............................. 153
WAT010823 ................................ 42
WAT011630 .............................. 280
PSS839515 .............................. 153
PSS839613 .............................. 153
WAT010830 ................................ 42
WAT011633 .............................. 194
PSS839516 .............................. 153
PSS839615 .............................. 153
WAT010835 ................................ 42
WAT011669 .............................. 194
PSS839517 .............................. 153
PSS839643 .............................. 153
WAT010873 .............................. 170
WAT011670 .............................. 156
PSS839518 .............................. 153
PSS839645 .............................. 153
WAT010910 ................................ 42
WAT011675 .............................. 156
PSS839520 .............................. 153
PSS839651 .............................. 153
WAT010932 .............................. 170
WAT011680 .............................. 156
PSS839521 .............................. 153
PSS839652 .............................. 153
WAT010935 .............................. 170
WAT011690 .............................. 171
PSS839522 .............................. 153
PSS839653 .............................. 153
WAT011695 .............................. 156
PSS839523 .............................. 153
PSS839655 .............................. 153
WAS207069 ............................. 295
WAT010947 .............................. 242
WAS290423 ............................. 306
WAT010950 .............................. 242
WAT011740 .............................. 161
PSS839525 .............................. 153
PSS839852 .............................. 153
WAT026-04 ............................... 323
WAT010960 .............................. 242
WAT011783 .............................. 227
PSS839526 .............................. 153
PSS839853 .............................. 153
WAT046-12 ............................... 323
WAT010965 .............................. 242
WAT011784 .............................. 227
PSS839527 .............................. 153
PSS845277 .............................. 153
WAT064-01 ............................... 228
WAT010986 .............................. 193
WAT011786 .............................. 234
PSS839528 .............................. 153
PSS845280 .............................. 153
WAT064-02 ............................... 228
WAT010987 .............................. 193
WAT011787 .............................. 234
PSS839530 .............................. 153
PSS845281 .............................. 153
WAT163-14 ............................... 286
WAT010988 .............................. 194
WAT011793 .............................. 159
PSS839531 .............................. 153
PSS845284 .............................. 153
WAT163-18 ....................... 286, 300
WAT011042 .............................. 296
WAT011794 .............................. 159
PSS839532 .............................. 153
PSS845285 .............................. 153
WAT005063 ............................ , 229
WAT011390 .............. 36, 38, 50, 51
WAT011795 ...................... 159, 175
PSS839533 .............................. 153
PSS845288 .............................. 153
WAT005070 ............................ , 229
WAT011400 ................................ 51
WAT011796 .............................. 159
PSS839535 .............................. 153
PSS845289 .............................. 153
WAT005079 .............................. 323
WAT011520 ...................... 170, 234
WAT011797 ...................... 159, 192
PSS839536 .............................. 153
PSS845292 .............................. 153
WAT005129 .............................. 195
WAT011525 ...................... 170, 234
WAT011798 ...................... 159, 192
PSS839537 .............................. 153
PSS845293 .............................. 153
WAT005130 .............................. 195
WAT011530 ...................... 170, 234
WAT011799 ...................... 159, 192
PSS839538 .............................. 153
PSS845296 .............................. 153
WAT005136 .............................. 229
WAT011535 ...................... 170, 234
WAT011800 ...................... 159, 192
PSS839540 .............................. 153
PSS845297 .............................. 153
WAT005137 .............. 195, 229, 229
WAT011540 ...................... 170, 234
WAT011801 ...................... 159, 192
350
Part Number Index
PSS845553 .............................. 153

PSS845554 .............................. 153
PSS845555 .............................. 153
PSS845556 .............................. 153
PSS845557 .............................. 153
PSS845558 .............................. 153
PSS845559 .............................. 153
PSS845560 .............................. 153
PSS845561 .............................. 153
PSS845562 .............................. 153
PSS845563 .............................. 153
PSS845564 .............................. 153
PSS845565 .............................. 153
PSS845566 .............................. 153
PSS845567 .............................. 153
PSS845568 .............................. 153
PSS845569 .............................. 153
PSS845570 .............................. 153
WAS037497 ............................. 301
WAS081140 ............................. 306
WAS081142 ............ 285, 286, 290,
................................ 306, 307, 308
WAS081333 ............................. 306
WAS081346 ............................. 306
WAS081347 ............................. 306
WAS081348 ............................. 306
www.waters.com
PaRT NUMbER INdEx

WAT011802 ...................... 159, 192
WAT020723 .............................. 192
WAT022384 ...................... 309, 323
WAT023620 ................................ 42
WAT025865 .............................. 277
WAT011803 ...................... 159, 192
WAT020731 .............................. 192
WAT022385 ...................... 309, 323
WAT023625 ................................ 42
WAT025866 .............................. 277
WAT011804 ...................... 159, 192
WAT020732 .............................. 192
WAT022400 .............................. 195
WAT023635 ................................ 42
WAT025867 .............................. 277
WAT011805 ...................... 159, 192
WAT020733 .............................. 192
WAT022440 .............................. 193
WAT023650 .............................. 159
WAT025868 .............................. 277
WAT011807 ...................... 159, 192
WAT020739 .............................. 192
WAT022441 .............................. 193
WAT023655 .............................. 156
WAT025869 .............................. 277
WAT011808 ...................... 159, 192
WAT020740 .............................. 192
WAT022453 .............................. 195
WAT024120 .............................. 295
WAT025870 .............................. 277
WAT011809 ...................... 159, 192
WAT020741 .............................. 192
WAT022454 .............................. 195
WAT025871 .............................. 277
WAT011810 ...................... 159, 192
WAT020770 .............................. 175
WAT022476 ................................ 72
WAT024310 ................... 47, 38, 50,

............................................ 51, 38
WAT011812 ...................... 159, 192
WAT020790 ...................... 157, 165
WAT022479 ................................ 72
WAT025028 .............................. 295
WAT020795 ...................... 157, 165
WAT022615 .............................. 301
WAT025050 .......................... 72, 76
WAT025873 .............................. 194
WAT011813 ...................... 159, 192

WAT011814 ...................... 159, 192
WAT020800 ...................... 157, 165
WAT022626 .............................. 301
WAT025051 .......................... 72, 76
WAT011815 ...................... 159, 192
WAT020805 ................................ 43
WAT022628 ...................... 300, 301
WAT025053C .............................. 72
WAT015199 .......................... 72, 76
WAT020810 ................................ 43
WAT022681 .............................. 163
WAT025053DV ............................ 72
WAT015199DV ............................ 76
WAT020815 ................................ 43
WAT022861 .......................... 72, 76
WAT025054C .............................. 72
WAT015207 .............................. 173
WAT020820 ................................ 43
WAT022934 .............................. 295
WAT025054DV ............................ 72
WAT015209 .............................. 170
WAT020825 ................................ 43
WAT025203 .............................. 295
WAT015220 ...................... 157, 165
WAT020830 ................................ 43
WAT022946 ..................... 295, 296,

........................................ 297, 300
WAT015401 .............................. 194
WAT020835 ................................ 43
WAT022995 .............................. 323
WAT025212 .............................. 295
WAT015490 .............................. 248
WAT020840 ................................ 43
WAT022996 .............................. 323
WAT025216 .............................. 295
WAT015674 ...................... 154, 320
WAT020845 ................................ 43
WAT022997 .............................. 323
WAT025263 .............................. 296
WAT015797 .............................. 195
WAT020850 ................................ 43
WAT022998 .............................. 323
WAT025296 .............................. 295
WAT015814 ...................... 193, 195
WAT020855 ................................ 43
WAT022999 ...................... 159, 323
WAT025297 ...................... 295, 296
WAT015831 .............................. 195
WAT021812 .............................. 323
WAT023331 .............................. 228
WAT025313 .............................. 323
WAT015833 .............................. 195
WAT021815 ...................... 307, 308
WAT023332 .............................. 228
WAT025360 .............................. 295
WAT015834 .............................. 195
WAT021816 ...................... 307, 308
WAT023333 .............................. 228
WAT025384 .............................. 295
WAT015835 .............................. 195
WAT021817 .............................. 323
WAT023336 .............................. 228
WAT015848 .............................. 195
WAT021901 .............................. 228
WAT023343 .............................. 228
WAT025531 ............. 285, 295, 296,

................................ 297, 298, 299
WAT015854 .............................. 195
WAT021902 .............................. 228
WAT023344 ........................ 47, 228
WAT025559 .............................. 296
WAT015859 .............................. 195
WAT021903 .............................. 228
WAT023345 .............................. 228
WAT025566 .............................. 323
WAT015860 .............................. 195
WAT021904 .............................. 228
WAT023349 .............................. 228
WAT019311 ...................... 193, 195
WAT021906 .............................. 228
WAT023370 .............................. 228
WAT025604 ..................... 195, 309,

........................................ 310, 311
WAT019876 .............................. 228
WAT021907 .............................. 228
WAT023371 .............................. 228
WAT025656 .............................. 295
WAT026973 ...................... 309, 323
WAT019877 .............................. 228
WAT021908 .............................. 195
WAT023372 .............................. 228
WAT025746 .............................. 295
WAT026974 .............................. 323
WAT019878 .............................. 228
WAT021910 .............................. 228
WAT023373 .............................. 228
WAT025820 ...................... 157, 191
WAT027047 .............................. 176
WAT019891 .............................. 228
WAT021927 .............................. 228
WAT023401 .............................. 320
WAT025821 ...................... 157, 191
WAT027048 .............................. 176
WAT019892 .............................. 228
WAT021945 .............................. 228
WAT023486 ...................... 298, 299
WAT025822 ...................... 157, 191
WAT027049 .............................. 176
WAT021946 .............................. 228
WAT023501 ................................ 42
WAT025823 ...................... 157, 191
WAT019893 .............................. 228
WAT027053 .............................. 176
WAT021947 .............................. 228
WAT023507 ................................ 42
WAT025828 ...................... 191, 158
WAT020321 .............................. 286
WAT027054 .............................. 176
WAT021950 .............................. 228
WAT023513 ................................ 42
WAT025829 ...................... 160, 191
WAT020500 ................................ 42
WAT027055 .............................. 176
WAT021951 .............................. 229
WAT023519 ................................ 42
WAT025832 ...................... 191, 158
WAT020505 ................................ 42
WAT027059 .............................. 176
WAT021992 .............................. 228
WAT023525 ................................ 42
WAT025834 ...................... 191, 158
WAT020510 ................................ 42
WAT025835 ...................... 160, 191
WAT027060 .............................. 176
WAT022033 .............................. 228
WAT023531 ................................ 42
WAT020515 ................................ 42
WAT023537 ................................ 42
WAT025841 .............................. 194
WAT027061 .............................. 176
WAT020520 ................................ 42
WAT022034 .............................. 228
WAT023543 ................................ 42
WAT025843 .............................. 193
WAT027065 .............................. 176
WAT020525 ................................ 42
WAT022035 .............................. 228
WAT023549 ................................ 42
WAT025844 .............................. 193
WAT027066 .............................. 176
WAT020530 ................................ 42
WAT022180 ...................... 193, 195
WAT023555 ................................ 42
WAT025845 .............................. 194
WAT027067 .............................. 176
WAT020535 ................................ 42
WAT022276 .............................. 195
WAT023561 ................................ 42
WAT025846 .............................. 194
WAT027071 .............................. 176
WAT020540 ................................ 42
WAT022280 .............................. 195
WAT023575 ................................ 42
WAT025848 .............................. 194
WAT027072 .............................. 176
WAT020545 ........................ 42, 230
WAT022281 .............................. 195
WAT023580 ................................ 42
WAT025852 .............................. 194
WAT027073 .............................. 176
WAT020550 ........................ 42, 230
WAT022282 .............................. 195
WAT023585 ................................ 42
WAT025853 .............................. 194
WAT027077 .............................. 176
WAT020587 .............................. 192
WAT022283 .............................. 195
WAT023590 ................................ 42
WAT025859 .............................. 277
WAT027078 .............................. 176
WAT020588 .............................. 192
WAT022297 .............................. 195
WAT023595 ................................ 42
WAT025860 .............................. 277
WAT027079 .............................. 176
WAT020594 .............................. 192
WAT022298 .............................. 195
WAT023600 ................................ 42
WAT025861 .............................. 277
WAT027083 .............................. 176
WAT020595 .............................. 192
WAT022299 .............................. 195
WAT023605 ................................ 42
WAT025862 .............................. 277
WAT027084 .............................. 176
WAT020721 .............................. 192
WAT022330 .............................. 323
WAT023610 ................................ 42
WAT025863 .............................. 277
WAT027085 .............................. 176
WAT020722 .............................. 192
WAT022365 .............................. 193
WAT023615 ................................ 42
WAT025864 .............................. 277
WAT027089 .............................. 176
www.waters.com
WAT025207 .............................. 295
WAT025872 .............................. 277

WAT025874 ...................... 160, 191
WAT025875 ...................... 158, 191
WAT025876 .............................. 194
WAT025877 .............................. 194
WAT025878 .............................. 194
WAT026014 .............................. 295
WAT026592 .............................. 295
WAT026613 .............................. 295
WAT026644 .............................. 295
WAT026745 ...................... 158, 165
WAT026750 ...................... 158, 165
WAT026755 ...................... 161, 165
WAT026760 ...................... 158, 165
WAT026765 .............................. 174
WAT026770 .............................. 174
WAT026804 .............................. 323
WAT026805 ...................... 309, 323
WAT026806 .............................. 323
WAT026807 .............................. 323
WAT026808 .............................. 323
WAT026809 .............................. 323
WAT026810 .............................. 323
Part Number Index
351
PaRT NUMbER INdEx

WAT027090 .............................. 176
WAT034122 .............................. 276
WAT036555 .............................. 277
WAT037830 .............................. 194
WAT039785 .............................. 227
WAT027091 .............................. 176
WAT034123 .............................. 276
WAT036561 .............................. 194
WAT037833 .............................. 194
WAT039786 .............................. 227
WAT027198 ...................... 158, 191
WAT034124 .............................. 276
WAT036570 .............................. 175
WAT037836 .............................. 194
WAT039787 .............................. 227
WAT027324 ...................... 158, 191
WAT034125 .............................. 276
WAT036613 .............................. 234
WAT037839 .............................. 194
WAT039790 .............................. 227
WAT027477 ...................... 160, 191
WAT034207 .............................. 280
WAT036770 ................................ 42
WAT037842 .............................. 194
WAT039791 .............................. 227
WAT027501 .............................. 228
WAT034208 .............................. 280
WAT036775 ................................ 42
WAT037845 .............................. 194
WAT042091 .............................. 285
WAT027502 .............................. 228
WAT034209 .............................. 280
WAT036780 ................................ 43
WAT037851 .............................. 194
WAT042200 .............................. 300
WAT027503 .............................. 228
WAT034210 .............................. 280
WAT036785 ................................ 42
WAT037854 .............................. 193
WAT042881 .............................. 306
WAT027506 .............................. 228
WAT034220 .............................. 162
WAT036790 ................................ 43
WAT037857 .............................. 193
WAT042885 .............................. 306
WAT027509 .............................. 195
WAT034221 .............................. 162
WAT036795 ................................ 43
WAT038216 .............................. 117
WAT042887 .............................. 306
WAT027518 .............................. 195
WAT034223 .............................. 162
WAT036800 ................................ 42
WAT038500 ...................... 157, 191
WAT043069 .............................. 310
WAT027519 .............................. 195
WAT034224 .............................. 162
WAT036805 ................................ 42
WAT038501 ...................... 157, 191
WAT043345 ................................ 43
WAT027525 .............................. 228
WAT034225 .............................. 162
WAT036810 ................................ 42
WAT038502 .............................. 194
WAT043350 ................................ 43
WAT027528 .............................. 228
WAT034227 .............................. 162
WAT036815 ................................ 43
WAT043355 ................................ 43
WAT027530 .............................. 228
WAT034236 .............................. 173
WAT036820 ................................ 42
WAT038503 .............................. 194

WAT038504 .............................. 194
WAT043360 ................................ 43
WAT027542 .............................. 228
WAT034238 .............................. 170
WAT038505 .............................. 194
WAT043365 ................................ 43
WAT027543 .............................. 228
WAT034243 .............................. 173
WAT038506 .............................. 194
WAT043370 ................................ 43
WAT027544 .............................. 228
WAT034244 .............................. 170
WAT038507 .............................. 194
WAT043375 ................................ 43
WAT027577 .............................. 193
WAT034245 .............................. 170
WAT038508 .............................. 194
WAT043380 ................................ 43
WAT027578 .............................. 193
WAT034276 .............................. 170
WAT038509 .............................. 194
WAT043385 ................................ 43
WAT027629 .............. 295, 296, 300
WAT034277 .............................. 170
WAT038510 .............................. 193
WAT043390 ................................ 43
WAT030541 .............................. 295
WAT034279 .............................. 170
WAT038511 .............................. 193
WAT043395 ................................ 43
WAT030543 .............................. 295
WAT034282 .............................. 170
WAT038512 .............................. 194
WAT043400 ................................ 43
WAT030632 .............................. 192
WAT034298 .............................. 173
WAT038514 .............................. 194
WAT043405 ................................ 43
WAT030633 .............................. 192
WAT035190 .............................. 295
WAT038516 .............................. 194
WAT043410 ................................ 42
WAT030634 .............................. 192
WAT035501 .............................. 173
WAT038518 .............................. 194
WAT043415 ................................ 42
WAT030653 .............................. 235
WAT035520 .............................. 237
WAT038520 .............................. 194
WAT043425 ................................ 43
WAT030654 .............................. 235
WAT035524 .............................. 276
WAT038522 .............................. 194
WAT043430 ................................ 43
WAT030697 .............................. 276
WAT035571 .............................. 159
WAT038524 .............................. 194
WAT043435 ................................ 43
WAT030698 .............................. 276
WAT035577 .............................. 171
WAT038526 .............................. 194
WAT043460 ................................ 42
WAT030699 .............................. 276
WAT035605 .............................. 276
WAT038528 .............................. 193
WAT043475 ................................ 42
WAT030852 .............................. 295
WAT035611 .............................. 276
WAT038530 .............................. 193
WAT043480 ................................ 42
WAT031788 .............................. 295
WAT035612 .............................. 276
WAT038564 .............................. 227
WAT043485 ................................ 43
WAT031790 .............................. 295
WAT035650 .............................. 227
WAT038567 .............................. 227
WAT044200 .............................. 274
WAT031795 .............................. 323
WAT035655 .............................. 227
WAT038570 .............................. 194
WAT044201 .............................. 274
WAT032472 .............................. 165
WAT035672 ........................ 55, 192
WAT038571 .............................. 194
WAT044202 .............................. 274
WAT033679 .............................. 295
WAT035674 .............................. 192
WAT038575 .............................. 194
WAT044203 .............................. 274
WAT033994 .............................. 193
WAT035676 .............................. 192
WAT038800 .............................. 228
WAT044204 .............................. 274
WAT033996 .............................. 195
WAT035706 .............................. 280
WAT038802 .............................. 228
WAT044205 .............................. 274
WAT033997 .............................. 195
WAT035707 .............................. 280
WAT038803 .............................. 228
WAT044206 .............................. 273
WAT034100 .............................. 276
WAT035708 .............................. 280
WAT038804 .............................. 228
WAT044207 .............................. 273
WAT034101 .............................. 276
WAT035709 .............................. 280
WAT038805 .............................. 228
WAT044208 .............................. 273
WAT034102 .............................. 276
WAT035711 .............................. 280
WAT037560 .............................. 320
WAT038806 .............................. 228
WAT044209 .............................. 273
WAT034104 .............................. 276
WAT035714 .............................. 280
WAT037663 .............................. 227
WAT038807 .............................. 228
WAT044210 .............................. 273
WAT034105 .............................. 276
WAT035876 .............................. 156
WAT037672 .............................. 194
WAT038846 ...................... 193, 195
WAT044211 .............................. 273
WAT034106 .............................. 276
WAT035877 .............................. 156
WAT037676 .............................. 194
WAT038849 ...................... 193, 195
WAT044212 .............................. 273
WAT034109 .............................. 276
WAT035880 ...................... 157, 165
WAT037680 .............................. 194
WAT038851 .............................. 195
WAT044213 .............................. 273
WAT034110 .............................. 276
WAT035884 .............................. 193
WAT037684 .............................. 194
WAT039550 .......................... 44, 45
WAT044214 .............................. 273
WAT034111 .............................. 276
WAT035943 .............................. 234
WAT037688 .............................. 194
WAT039570 .............................. 227
WAT044215 .............................. 273
WAT034112 .............................. 276
WAT035946 .............................. 234
WAT037692 .............................. 194
WAT039575 .............................. 227
WAT044216 .............................. 273
WAT034116 .............................. 276
WAT035955 .............................. 235
WAT037696 .............................. 194
WAT039625 .............................. 227
WAT044217 .............................. 273
WAT034117 .............................. 276
WAT035970 .............................. 227
WAT037700 .............................. 194
WAT039630 .............................. 227
WAT044218 .............................. 273
WAT034118 .............................. 276
WAT035980 .............................. 227
WAT037704 .............................. 193
WAT039780 .............................. 227
WAT044219 .............................. 273
WAT034119 .............................. 276
WAT036545 .............................. 230
WAT037708 .............................. 193
WAT039782 .............................. 227
WAT044220 .............................. 273
WAT034120 .............................. 276
WAT036554 .............................. 277
WAT037712 .............................. 194
WAT039783 .............................. 227
WAT044221 .............................. 272
352
Part Number Index
WAT036865 ...................... 160, 165

WAT036870 ...................... 160, 165
WAT036875 ...................... 160, 165
WAT036905 ................................ 43
WAT036910 ................................ 43
WAT036915 ................................ 43
WAT036920 ................................ 43
WAT036925 ................................ 43
WAT036930 ................................ 43
WAT036935 ................................ 42
WAT036940 ................................ 42
WAT036945 ................................ 43
WAT036950 ................................ 43
WAT036954 .............................. 170
WAT036970 .............................. 156
WAT036975 .............................. 156
WAT036980 .............................. 156
WAT036985 .............................. 156
WAT037046 .............................. 304
WAT037047 .............................. 304
WAT037072 .............................. 304
WAT037090 .............................. 304
WAT037372 .............................. 285
WAT037373 .............................. 285
WAT037500 .......................... 44, 45
WAT037520 .............................. 156
WAT037525 ..................... 156, 158,
........................................ 165, 170
www.waters.com
PaRT NUMbER INdEx

WAT044222 .............................. 272
WAT045890 .............................. 272
WAT051810 ................................ 42
WAT054260 ........ 47, 38, 50, 51, 36
WAT054615 ................................ 43
WAT044223 .............................. 272
WAT045895 .............................. 272
WAT051820 ................................ 42
WAT054265 .......................... 44, 47
WAT054620 ................................ 43
WAT044224 .............................. 272
WAT045900 .............................. 272
WAT051900 ................................ 42
WAT054270 .............................. 146
WAT054625 ................................ 43
WAT044225 .............................. 272
WAT045905 .............................. 146
WAT051910 ................................ 42
WAT054275 .............................. 146
WAT054630 ................................ 43
WAT044226 .............................. 272
WAT045915 .............................. 273
WAT051960 ................................ 42
WAT054392 .............................. 280
WAT054635 ................................ 43
WAT044227 .............................. 272
WAT045920 .............................. 273
WAT052119 .............................. 301
WAT054405 .............. 272, 273, 274
WAT054640 ................................ 43
WAT044228 .............................. 272
WAT045925 .............................. 273
WAT052220 .............................. 301
WAT054410 .............. 272, 273, 274
WAT054645 ................................ 43
WAT044229 .............................. 272
WAT045930 .............................. 273
WAT052230 .............................. 301
WAT054415 .............. 272, 273, 274
WAT054650 ................................ 43
WAT044230 .............................. 272
WAT045935 .............................. 273
WAT052586 ...... 285, 286, 307, 308
WAT054420 .......................... 44, 46
WAT054655 ................................ 43
WAT044231 .............................. 272
WAT045940 .............................. 273
WAT052587 .............................. 296
WAT054434 .............................. 146
WAT054660 ................................ 43
WAT044232 .............................. 272
WAT045945 .............................. 273
WAT052669 .............................. 302
WAT054435 .............................. 146
WAT054665 ................................ 43
WAT044233 .............................. 272
WAT045950 .............................. 273
WAT052671 .............................. 301
WAT054438 .............................. 146
WAT054670 ................................ 43
WAT044234 .............................. 272
WAT045955 .............................. 273
WAT052673 .............................. 300
WAT054440 .............................. 146
WAT054675 ................................ 43
WAT044235 .............................. 272
WAT045960 .............................. 273
WAT052675 .............................. 300
WAT054442 .............................. 146
WAT054680 ................................ 43
WAT044236 .............................. 272
WAT045965 .............................. 273
WAT052705 ................................ 43
WAT054444 .............................. 146
WAT054685 ................................ 43
WAT044237 .............................. 272
WAT045970 .............................. 273
WAT052710 ................................ 42
WAT044238 .............................. 272
WAT054446 .............................. 146
WAT045975 .............................. 273
WAT044239 .............................. 272
WAT052715 ................................ 43
WAT054448 .............................. 146
WAT045980 .............................. 273
WAT044240 .............................. 272
WAT052720 ................................ 42
WAT054450 .............................. 146
WAT045985 .............................. 273
WAT044241 .............................. 272
WAT052725 ................................ 42
WAT054452 .............................. 146
WAT045995 .............................. 146
WAT044243 .............................. 156
WAT052735 .............................. 156
WAT054454 .............................. 146
WAT046800 .............................. 274
WAT044245 .............................. 156
WAT052740 .............................. 156
WAT054456 .............................. 146
WAT046805 .............................. 274
WAT044250 .............................. 175
WAT052745 .............................. 156
WAT054460 .............................. 272
WAT046810 .............................. 274
WAT044255 .............................. 157
WAT052770 ...................... 156, 157
WAT054464 .............................. 272
WAT046815 .............................. 274
WAT044355 .............................. 170
WAT054466 .............................. 272
WAT046820 .............................. 274
WAT052775 .............................. 156
WAT044360 .............................. 157
WAT046825 .............................. 274
WAT052780 .............................. 156
WAT054468 .............................. 272
WAT044370 .............................. 158
WAT046830 ...................... 157, 164
WAT052785 .............................. 156
WAT054470 .............................. 272
WAT044375 .............................. 156
WAT046835 ...................... 157, 164
WAT052790 .............................. 156
WAT054474 .............................. 272
WAT044380 ...................... 157, 164
WAT046840 ...................... 157, 164
WAT052795 .............................. 156
WAT054475 .............................. 273
WAT044385 .......................... 38, 51
WAT046845 ...................... 157, 164
WAT052800 .............................. 156
WAT054476 .............................. 273
WAT044395 .......................... 38, 51
WAT046850 ...................... 158, 164
WAT052805 .............................. 156
WAT054480 .............................. 273
WAT044445 .............................. 156
WAT046855 ...................... 158, 164
WAT052810 .............................. 156
WAT054488 .............................. 274
WAT044455 .............................. 156
WAT046860 ...................... 160, 164
WAT052834 .............................. 156
WAT054490 .............................. 274
WAT044460 .............................. 156
WAT046865 ...................... 158, 164
WAT052840 .............................. 156
WAT054494 .............................. 274
WAT044480 ...................... 158, 164
WAT046875 ...................... 160, 164
WAT052845 .............................. 156
WAT054500 ................................ 43
WAT045424 ..................... 292, 293,

........................................ 295, 302
WAT046880 ...................... 160, 164
WAT052850 .............................. 156
WAT054505 ................................ 43
WAT052855 .............................. 156
WAT054515 ................................ 43
WAT052860 .............................. 158
WAT054520 ................................ 43
WAT052874 .............................. 241
WAT054525 ................................ 43
WAT052875 .............................. 241
WAT054530 ................................ 43
WAT052880 .............................. 241
WAT054535 ................................ 43
WAT052885 .............................. 241
WAT054540 ................................ 43
WAT052890 .............................. 241
WAT054545 ................................ 43
WAT052895 .............................. 241
WAT054550 ................................ 43
WAT045427 .............. 292, 293, 295

WAT045800 .............................. 272
WAT045805 .............................. 272
WAT045810 .............................. 272
WAT045815 .............................. 272
WAT046885 ...................... 160, 164

WAT046890 ...................... 160, 164
WAT046895 ...................... 164, 170
WAT046905 ............. 157, 158, 160,
................................ 161, 163, 170
WAT054690 ................................ 43
WAT054695 ................................ 43
WAT054700 ................................ 43
WAT054705 ................................ 43
WAT054710 ................................ 43
WAT054715 ................................ 43
WAT054720 ................................ 43
WAT054725 ................................ 43
WAT054730 ................................ 43
WAT054735 ................................ 43
WAT054740 ................................ 43
WAT054870 .............................. 156
WAT054880 .............................. 156
WAT054890 .............................. 156
WAT054925 ................................ 43
WAT054930 ................................ 43
WAT054935 ................................ 43
WAT054940 ................................ 43
WAT054945 ................................ 43
WAT054955 ................................ 42
WAT054960 ................................ 42
WAT054965 ................................ 42
WAT054975 ................................ 42
WAT054980 ................................ 42
WAT054985 ................................ 42
WAT055426 ...................... 287, 291
WAT055629 .............................. 300
WAT045825 .............................. 272
WAT046910 ............. 157, 158, 160,

........................ 161,163, 165, 213,
............................ 36, 38, 160, 234
WAT045830 .............................. 272
WAT046915 ...................... 161, 164
WAT054200 .............................. 146
WAT054555 ................................ 43
WAT045835 .............................. 272
WAT046920 ...................... 161, 164
WAT054205 .............................. 146
WAT054560 ................................ 43
WAT045840 .............................. 272
WAT046955 .............................. 146
WAT054210 .............................. 146
WAT054565 ................................ 43
WAT045845 .............................. 272
WAT046970 .............................. 146
WAT054215 .............................. 146
WAT054570 ................................ 43
WAT045850 .............................. 272
WAT046980 .............................. 146
WAT054220 .............................. 146
WAT054575 ................................ 43
WAT045855 .............................. 272
WAT047205 .......................... 44, 46
WAT054225 ...................... 146, 164
WAT054580 ................................ 43
WAT045860 .............................. 272
WAT047210 .............................. 146
WAT054230 .............................. 146
WAT054585 ................................ 43
WAT045865 .............................. 272
WAT047220 .......................... 44, 47
WAT054235 .............................. 146
WAT054590 ................................ 43
WAT057235 ............. 286, 287, 306,

........................................ 307, 309
WAT045870 .............................. 272
WAT048160 .............. 36, 38, 50, 51
WAT054240 .............................. 146
WAT054595 ................................ 43
WAT057337 .............................. 307
WAT045875 .............................. 272
WAT051041 .............................. 323
WAT054245 .............................. 146
WAT054600 ................................ 43
WAT057460 .............................. 307
WAT045880 .............................. 272
WAT051052 .............................. 323
WAT054250 ...................... 146, 164
WAT054605 ................................ 43
WAT057461 .............................. 307
WAT045885 .............................. 272
WAT051800 ................................ 42
WAT054255 .............................. 146
WAT054610 ................................ 43
WAT057462 .............................. 307
WAT045820 .............................. 272
www.waters.com
WAT055631 .............................. 300

WAT055632 .............................. 300
WAT055633 .............................. 300
WAT056920 .............................. 156
WAT056955 .............................. 146
WAT056970 .............................. 194
WAT056975 .............................. 146
WAT057112 .............................. 286
Part Number Index
353
PaRT NUMbER INdEx

WAT057463 .............................. 307
WAT069581 .............................. 295
WAT084630 .............................. 193
WAT088431 .............................. 323
WAT097962 ........................ 84, 105
WAT057919 .............................. 307
WAT069583 .............................. 295
WAT084634 .............................. 193
WAT088432 .............................. 323
WAT097963 ................................ 84
WAT057920 .............................. 307
WAT070122 .............................. 299
WAT084636 .............................. 193
WAT088494 .............................. 195
WAT097964 ........................ 84, 105
WAT057921 .............................. 307
WAT071406 .............................. 301
WAT084720 .............................. 193
WAT088500 ...................... 191, 158
WAT097965 ................................ 84
WAT057922 .............................. 307
WAT072030 .......................... 72, 76
WAT084730 .............................. 193
WAT088561 .............................. 323
WAT106001 .............................. 148
WAT058839 ................................ 50
WAT072289 ................................ 72
WAT085101 .............................. 166
WAT088563 .............................. 323
WAT106005 .............................. 146
WAT058840 ................................ 50
WAT072294 ................................ 72
WAT085102 ................................ 83
WAT088919 .............................. 195
WAT106008 .............................. 148
WAT058851 ................................ 50
WAT072704 .......................... 72, 76
WAT085103 .............................. 166
WAT089592 ...................... 194, 230
WAT106011 .............................. 146
WAT058853 ................................ 84
WAT072704DV ............................ 76
WAT085110 .............................. 166
WAT091631 .............................. 194
WAT106048 .............................. 148
WAT058871 ................................ 50
WAT072708 .......................... 72, 76
WAT085113 ................................ 83
WAT091648 ...................... 160, 191
WAT106052 .............................. 146
WAT058874 .......................... 70, 74
WAT072711 .......................... 72, 76
WAT085114 .......................... 83, 50
WAT091681 .............................. 242
WAT106056 .............................. 146
WAT058875 .......................... 70, 74
WAT072714 .......................... 72, 76
WAT085115 .......................... 83, 50
WAT091784 .............................. 194
WAT106060 .............................. 148
WAT058876 ................................ 74
WAT072919 .............................. 301
WAT085123 ................................ 83
WAT094003 .............................. 295
WAT106127 ...................... 146, 164
WAT058882 .......................... 34, 36
WAT073005 .......................... 72, 76
WAT085140 .............................. 166
WAT094170 .............. 70, 71, 74, 75
WAT106128 ...................... 146, 164
WAT058883 .......................... 34, 36
WAT073109 .............. 293, 295, 302
WAT085142 .............................. 166
WAT094170DV ............................ 74
WAT075215 .............................. 323
WAT085144 .............................. 166
WAT106129 ...................... 148, 164
WAT058886 ................................ 74
WAT076152 .............. 285, 288, 290
WAT085188 .............................. 170
WAT106151 ...................... 150, 214
WAT058930 .............................. 280
WAT094171 ................... 68, 70, 71,

...................................... 73, 74, 75
WAT058931 .............................. 280
WAT077342 ..................... 292, 293,

........................................ 295, 302
WAT085250 .............................. 234
WAT094171DV ............................ 74
WAT085466 .............................. 166
WAT094172 ................................ 74
WAT077343 ..................... 292, 293,

........................................ 295, 302
WAT085500 .............................. 173
WAT094174 ................................ 74
WAT094219 .......................... 71, 75
WAT078515 ................................ 72
WAT085501 .............................. 173

WAT085650 .............................. 170
WAT094219DV ...................... 71, 75
WAT085670 .............................. 193
WAT094220 .......................... 71, 75
WAT085672 .............................. 193
WAT094220DV ...................... 71, 75
WAT085711 .............................. 161
WAT094222 .......................... 71, 75
WAT085720 .............................. 194
WAT094222DV ...................... 71, 75
WAT085721 .............................. 194
WAT094223 .......................... 71, 75
WAT085722 .............................. 194
WAT094223DV ...................... 71, 75
WAT085724 .............................. 194
WAT094225 .......................... 34, 36
WAT085824 ...................... 161, 165
WAT094226 .......................... 34, 36
WAT085825 ...................... 161, 165
WAT094231 .............................. 146
WAT085826 ...................... 161, 165
WAT094234 .............................. 146
WAT086016 .............................. 161
WAT094237 .............................. 146
WAT086342 .............................. 193
WAT094240 .............................. 146
WAT086344 ...................... 156, 173
WAT094243 .............................. 148
WAT086609 .............................. 158
WAT094245 .............................. 148
WAT086680 .............................. 158
WAT094248 .............................. 148
WAT086684 .............................. 158
WAT094251 .............................. 148
WAT086688 .............................. 158
WAT094254 .............................. 148
WAT086692 .............................. 160
WAT094257 .............................. 148
WAT058942 ........................ 209, 38

WAT058943 ................................ 38
WAT058951 .......................... 34, 36
WAT058958 ................................ 38
WAT058959 ................................ 38
WAT058961 .............................. 146
WAT058965 .............................. 146
WAT058969 .............................. 148
WAT058973 .............................. 146
WAT058977 .............................. 146
WAT060051 .............................. 323
WAT060304 .............................. 295
WAT060305 .............................. 295
WAT060307 .............................. 295
WAT060308 .............................. 295
WAT060495 .............................. 295
WAT062341 ...................... 292, 293
WAT062479 ...................... 292, 293
WAT063300 .......................... 70, 74
WAT078723 .............................. 302

WAT078727 .............................. 302
WAT080013 .............................. 234
WAT080046 .............................. 165
WAT080100 .............................. 193
WAT081157 .............................. 306
WAT081158 .............................. 306
WAT081159 .............................. 306
WAT081321 .............................. 306
WAT082708 .............................. 229
WAT082734 .............................. 229
WAT082745 .................. 36, 38, 229
WAT082853 .............................. 195
WAT082887 .............. 193, 195, 175
WAT082888 .............................. 195
WAT082892 .............................. 195
WAT106154 ...................... 150, 214

WAT106157 ...................... 150, 214
WAT106160 .............................. 150
WAT106163 .............................. 150
WAT106166 ...................... 150, 164
WAT106169 .............................. 150
WAT106172 ...................... 150, 214
WAT106175 .............................. 150
WAT106178 .............................. 150
WAT106181 .............................. 150
WAT106184 .............................. 150
WAT106187 .............................. 150
WAT106190 .............................. 150
WAT106193 .............................. 150
WAT106202 .......................... 35, 36
WAT106209 ...................... 150, 214
WAT200500 .......................... 83, 84
WAT200501 ................................ 84
WAT200502 .......................... 83, 84
WAT200503 ................................ 84
WAT200504 .......................... 83, 84
WAT063300DV ...................... 70, 74
WAT084038 .............................. 170
WAT066200 .............................. 146
WAT084040 ...................... 158, 191
WAT066204 .............................. 146
WAT084078 .............................. 323
WAT066210 .............................. 146
WAT087141 .............................. 309
WAT094260 .............................. 148
WAT084175 ...................... 160, 191
WAT066215 .............................. 146
WAT088027 .............................. 195
WAT094263 .............................. 148
WAT084176 ...................... 191, 158
WAT066220 .............................. 146
WAT088043 .............................. 227
WAT094266 .............................. 148
WAT084177 ...................... 191, 158
WAT066224 .............................. 146
WAT088044 .............................. 227
WAT094269 .............................. 148
WAT084178 ...................... 191, 158
WAT066225 .............................. 190
WAT088070 ...................... 158, 165
WAT094272 .............................. 148
WAT084179 ...................... 191, 158
WAT066228 .............................. 190
WAT088084 .............................. 295
WAT094275 .............................. 148
WAT084189 .............................. 166
WAT088108 .............................. 242
WAT094278 .............................. 148
WAT200514 .......................... 83, 84
WAT066230 .............................. 190
WAT084198 .............................. 166

WAT084199 .............................. 166
WAT200516 .......................... 83, 84
WAT066240 .............................. 190
WAT088119 .............................. 242
WAT097194 ............. 286, 287, 306,

........................................ 309, 310
WAT200515 ................................ 84
WAT066235 .............................. 190
WAT088112 .............................. 242
WAT084282 .............................. 166
WAT097332 ...................... 310, 311
WAT200517 ................................ 84
WAT066245 .............................. 190
WAT088122 ...................... 240, 241
WAT084283 .............................. 166
WAT097410 .............................. 310
WAT200518 .......................... 83, 84
WAT066260 .............................. 146
WAT088123 .............................. 242
WAT084560 .......................... 36, 38
WAT097944 ................................ 38
WAT200519 ................................ 84
WAT066265 .............................. 146
WAT088131 .............................. 242
WAT084601 .............................. 234
WAT097945 ................................ 38
WAT200520 .......................... 83, 84
WAT066285 .............................. 190
WAT084620 .............................. 193
WAT088141 ............. 158, 160, 161,

............................... 165, 174, 175,
WAT097946 ................................ 38
WAT200521 ................................ 84
WAT066288 .............................. 190
WAT084624 ...................... 193, 175
WAT088223 .............................. 295
WAT097954 .............................. 163
WAT200522 .......................... 83, 84
WAT069511 .............................. 295
WAT084626 .............................. 193
WAT088430 .............................. 323
WAT097958 .............. 163, 165, 258
WAT200523 ................................ 84
354
Part Number Index
WAT200505 ................................ 84
WAT200506 .......................... 83, 84
WAT200507 ................................ 84
WAT200508 ................................ 83
WAT200510 .......................... 83, 84
WAT200511 ................................ 84
WAT200512 .......................... 83, 84
WAT200513 ................................ 84
www.waters.com
PART NUMBER INDEX

WAT200524 .......................... 83, 84
WAT200525 ................................ 84
WAT200529 .......................... 83, 84
WAT200530 ................................ 83
WAT200531 ................................ 83
WAT200532 ................................ 83
WAT200533 ................................ 83
WAT200534 ................................ 83
WAT200535 ................................ 83
WAT200536 ................................ 83
WAT200537 ................................ 83
WAT200538 ................................ 83
WAT200539 ................................ 83
WAT200540 ................................ 83
WAT200541 ................................ 83
WAT200542 ................................ 83
WAT200543 ................................ 83
WAT200544 ................................ 83
WAT200545 ................................ 83
WAT200546 ................................ 83
WAT200547 ................................ 83
WAT200556 ................................ 84
WAT200557 ................................ 84
WAT200558 ................................ 84
WAT200559 ................................ 84
WAT200560 ................................ 84
WAT200561 ................................ 84
WAT200562 ................................ 84
WAT200563 ................................ 84
WAT200564 ................................ 84
WAT200565 ................................ 84
WAT200566 ................................ 83
WAT200606 .......................... 38, 50
WAT200607 .......................... 38, 50
WAT200608 .......................... 38, 50
WAT200609 .......................... 38, 50
WAT200620 .............................. 146
WAT200624 .............................. 146
WAT200625 .............................. 146
WAT200630 .............................. 146
WAT200632 .............................. 146
WAT200650 .............................. 146
WAT200655 .............................. 148
WAT200658 .............................. 148
WAT200661 .............................. 148
WAT200662 .............................. 148
WAT200665 .............................. 148
WAT200670 .............................. 148
WAT200675 ...................... 148, 164
WAT200677 .......................... 38, 50
WAT200678 ................................ 50
WAT200679 ................................ 50
WAT200680 ................................ 50
WAT200681 ................................ 50
WAT200685 ................................ 50
WAT200686 ................................ 50
WAT200687 ................................ 50
WAT200688 ................................ 50
WAT200689 ................................ 50
WAT200690 ................................ 50
WAT200691 ................................ 50
WAT200800 ................................ 84
WAT200801 ................................ 84
WAT200802 ................................ 84
WAT200803 ................................ 84
WAT200804 ................................ 84
WAT200805 ................................ 84
WAT200806 ................................ 84
WAT200807 ................................ 84
WAT200808 ................................ 84
WAT200809 ................................ 84
WAT200810 ................................ 84
WAT200811 ................................ 84
WAT200812 ................................ 84
WAT200813 ................................ 84
WAT200814 ................................ 84
WAT200815 ................................ 84
WAT200816 ................................ 84
WAT200817 ................................ 84
WAT200818 ................................ 84
WAT200819 ................................ 84
WAT200820 ................................ 84
WAT200821 ................................ 84
WAT200822 ................................ 84
WAT200823 ................................ 84
WAT200824 ................................ 84
WAT200825 ................................ 84
WAT200826 ................................ 84
WAT200827 ................................ 84
WAT200828 ................................ 84
WAT200829 ................................ 84
WAT200830 ................................ 84
WAT200831 ................................ 84
WAT200832 ................................ 84
WAT200833 ................................ 84
WAT200834 ................................ 84
WAT200835 ................................ 84
WAT200836 ................................ 84
WAT200838 ................................ 84
WAT200839 ................................ 84
WAT200840 ................................ 84
WAT200841 ................................ 84
WAT200842 ................................ 84
WAT200843 ................................ 84
WAT200844 ................................ 84
WAT200845 ................................ 84
WAT200846 ................................ 84
WAT201549 .............................. 213
WAT210579 .............................. 148
WAT210582 .............................. 148
WAT210585 .............................. 148
WAT210588 .............................. 148
WAT210591 .............................. 148
WAT210594 .............................. 148
WAT210685 ................................ 74
WAT241039 .............................. 323
WAT248059 .............................. 146

WAT248072 .............................. 146
WAT270328 .............................. 295
WAT270868 .............. 290, 309, 310
WAT270926 .............................. 288
WAT270927 .............................. 295
WAT270938 .............................. 295
WAT270939 .............................. 295
WAT270940 .............................. 295
WAT270941 .............................. 295
WAT270944 .............................. 292
WAT270946C ........................ 70, 74
WAT270946DV ...................... 70, 74
WAT270959 .............................. 295
WAT270964 .............................. 295
WAT271017 .............................. 295
WAT271018 .............................. 295
WAT271019 .............................. 295
WAT271066 .............. 292, 293, 295
WAT271067 .............. 292, 293, 295
WAT272080 .............................. 304
WAT272617 .............................. 304
WAT272620 .............................. 304
WAT272623 .............................. 304
WAT411410 .............................. 286
Trademarks of Waters Corporation:

Bondapak, Porasil, Styragel, Accell, AccQTag, AccQ-Fluor, AccuPure, AccuSep, ACQUITY, ACQUITY UPLC, ACQUITY UltraPerformance LC, ActiveWell, Alliance, ALPS, Atlantis, AutoPurification,
AutoTag, AutoSpec, AutoSpec Premier, BEH Tec hnology, BioSuite, Bondapak, Critical Clean, CapLC, C-Gel, Clear-100, Connections, Delta-Pak, Dextro-Pak, DIOS-target, DisQuE, DNPH-Silica,
eCord, Empower, EnantioSelect, Envirogel, FractionLynx, GCT, GCT Premier, Gen-Pak, Guard-Pak, HSPgel, IC-Pak, Connections INSIGHT, Integrity, iRequest, IonSABRE, IonSelect, IS, LC-MALDIprep,
LCT, LCT Premier, LectraBond, LockSpray, M@LDI, MALDI micro MX, Maldi Tof, MassLynx, MassPREP, MassT RAK, nanoACQUITY UPLC, NanoEase, Nova-Pak, Oasis, OBD, PerformancePLUS, PIC, PicoTag,
Platform LC, Porapak, Porasil, PrepLC, PrepPak, ProteinLynx, Protein-Pak, Q-Tof, Q-Tof micro, Q-Tof Premier, Q-Tof Ultima, QuanLynx, Quattro, Quattro LC, Quattro micro, Quattro micro GC,
Quattro Premier, Quattro Premier XE, Quattro Ultima, Quattro Ultima Pt, Radial-Pak, RapiGest, Resolve, SAM, Sentry, Sep-Pak, Silica-Pak, Styragel, Sugar-Pak, SunFire, Symmetry, Symmetry300,
SymmetryPrep, SymmetryShield, T hermabeam, T he Science of W hats Possible, Ultrahydrogel, Ultrastyragel, UPLC, UltraPerformance LC, VanGuard, Waters, Waters Quality Parts, Waters Spherisorb,
XBridge, Xevo, X PoSure, XSelect, XTerra and ZQ
All other trademarks in the catalog are acknowledged.

ACE
ACT
Gemini
Phenomenex, Inc.
SLIPFREE
Keystone Scientific, Inc.
Acrodisc
Pall Life Sciences
Inertsil
GL SCiences
Supor
Pall Life Sciences
AcroPrep
Pall Life Sciences
Inter-Seal
Integrated Liner Technologies, Inc.
Symbiosis
Spark Holland
Aquasil
T hermo Fisher Scientific
Luna
Phenomenex, Inc.
Synergi
T hermo Fisher Scientific
Bottom-of-the-Bottle
Upchurch Scientific
OptiPak
Optimize Technologies
TaqMan
Roche Molecular Systems, Inc
Capcell
Shiseido Co., Ltd.
Page
J&W
VICAM
VICAM
Cheminert
VICA
Partisil
W hatman
Z-Spin
Pall Life Sciences
Derlin
E.I. du Pont de Nemours

and Company
PEEK
Victrex PLC
ZipTIP
Millipore Corporation
Prospekt
Spark Holland
Zorbax
Florisil
US Silica Corporation.
Shodex
Showa Denko
Zorbax Extend
FPLC
Pharmacia LKB
Sirocco
Pall Life Sciences
www.waters.com
Part Number Index
355
How to order
by Telephone
by Fax
by Mail
To place an order, request price and availability, delivery status/

expediting an order, locate your local sales representative and all other
customer service, 1-800-252-4752
508-482-4820 (U.S.)
Waters Corporation
Mail Stop: CM
5 Technology Drive, Milford,
Massachusetts 01757
1. Can I order by phone or fax?
Yes, Monday through Friday from 8:00 a.m. 6:00 p.m. E.S.T., just
call our toll free number, 1-800-252-4752, press 1, press 1, or fax
your order to 508-482-4820 (U.S.) or 905-678-2350 (Canada).
W hen ordering, please have your purc hase order number or credit card
number, the catalog number and description for eac h product. We will
also need your shipping address, including an attention line if applicable, and a complete billing address. In addition, please be sure to give
us your daytime telephone number so that we can reac h you promptly
it theres a follow-up question about your order.
2. How do I release product(s) against my
Chemical Products Standing Order?
To release product(s) from your c hemical products standing/blanket

order, call 1-800-252-4752, press 1, then press 5 for a direct line or
for an alternate who will make arrangements to release the product(s)
from your order or answer any questions that you may have. You can
also fax your request to 508-482-2672.
3. W hen will I receive my order?
T he majority of items in this catalog are in stock and will normally be

shipped within 24 hours of receipt of your order. We can also arrange
blanket or standing orders to meet your needs.
905-678-2350 (Canada)
4. How will my order be shipped?
You select the transportation best suited to your needs. Waters offers
FedEx and UPS as our standard carriers offering 3-day ground saver,
Priority 1, 10:30 a.m. Next Day; Standard, 3:00 p.m. Next Day; and
Economy Two-Day Service.
5. W hat are the payment terms?
Net 30 days. Shipment on U.S. orders is FOB, Franklin, MA.

6. How do I return an item?
A return authorization number (RA#) must be obtained to return an item.

If an item is being returned for a warranty reason, please contact Technical
Support at 1-800-252-4752, extension 8360. If the return is being
requested due to an incorrect order, shipping error, or damage claim, please
contact Customer Support at 1-800-252-4752, extension 8365.
Outside the U.S.A., please refer to opposite page for the address and
telephone number of your local Waters office, or visit www.waters.com
SaleS OfficeS
Australia
Waters Australia Pty. Limited
Unit 3, 38-46 South Street
P.O Box 84
Rydalmere BC NSW 2116
Tel: 2 9933 1777
Fax: 2 9898 1455
Dear Colleague,
We are pleased to present the 2011 Waters Quality Parts,

Chromatography Columns and Supplies Catalog.
Our catalog contains todays leading separations technology products. With over 50 years of market
leadership we remain committed to the development and manufacture of products that solve todays most
challenging separations problems to enable our customers success. We are driven by our core mission to
provide enabling technologies in products that set the industry standard for performance and reproducibility.
Waters provides the industrys broadest and deepest product offering manufactured to industry
benc hmark quality requirements by our world-class manufacturing organization and supported by our
highly skilled sales and support teams.
We invite you to visit our new Online Order Center at www waters.com/order. T he online ordering
experience has been improved to make it easier and faster for you to obtain pricing and availability when
buying products.
T his year for the fist time our catalog is available in digital form. You may access and download it at
www.waters.com/catalog.
T his past year we launched several innovative, enabling products including our new CSH technology
that is the basis for the XSelect brand of HPLC columns and the ACQUITY CSH brand of UPLC columns,
the Ostro Sample Preparation plate for the removal of phospholipids in biological samples and the
Viridis line of columns for supercritical fluid chromatography.
To learn more about how we can help you achieve your objectives please telephone one of our 54 worldwide offices and interact with our highly trained account specialists.
Austria and European Export

(Central South Eastern Europe,
CIS and Middle East)
Waters Ges.m.b.H.
Hietzinger Haupstrasse 145
A-1130 Vienna
Tel: 431 877 18 07
Fax: 431 877 18 08
Belgium
Waters S.A.-N.V.
ConneXion Business Park
Brusselsesteenweg 500
B-1731 Zellik
Tel: 32 2 726 1000
Fax: 32 2 726 1100
Brazil
Waters Technologies Do
Brasil LTDA
Alameda Tocantins, 27o. andar
Alphaville
06455-020 Barueri, Brazil
Tel: 55 11 4134 3788
Fax: 55 11 4193 6133
Canada
Waters Limited
6427 Northam Drive
Mississauga
Ontario L4V 1J2
Tel: 800 252 4752
Fax: 905 678 9237
China
Waters Technologies
(Shanghai) Limited
Block 41, Room 01
1387 Zhangdong Road
Pudong New District
Shanghai 201203, PR China
Tel: 86 21 6879 5888
Fax: 86 21 6879 4588
CIS/Russia
Waters Ges.m.b.H.
Representation Office
ul. Miklukho-Maklaia 16/10
117871 Moscow
Tel: +7 495 727 4490/290 9737
Fax: +7 495 336 7000
Czech Republic
Waters Ges.m.b.H
Psohlavcu 43
147 00 Prague 4
Tel: 42 02 617 11384
Fax: 42 02 617 11386
Denmark
Waters A/S
Baldersbuen 46
2640 Hedehusene
Tel: 45 46 59 8080
Fax: 45 46 59 8585
Finland
Waters Finland
Kutomotie 16
00380 HELSINKI
FINLAND
Tel: 358 9 5659 6288
Fax: 358 9 5659 6282
Puerto Rico
Waters Corp PR Branch
Ave. Gautier Benitez #230
Edif. Video Avenue 2nd Floor
Caguas,PR 00725
Tel: 787 747 8445
Fax: 787 747 8448
Italy
Waters S.P.A.
Via Achille Grandi, 27
20090 Vimodrone MI
Tel: 39 02 265 0983
Fax: 39 02 250 1827
Singapore
Waters Asia Limited
1 Science Park Road
#02-01/06 The Capricorn
Singapore Science Park II
Singapore 117528
Tel: 65 6593 7100
Fax: 65 6873 0733
Japan
Nihon Waters K.K.
Dai-5. Koike Bldg.
1-3-12 Kita-shinagawa
Shinagawa-ku, Tokyo 140-0001
Tel: (81) 3 3471 7191
Fax: (81) 3 3471 7118
France
Waters S.A
B.P. 608
78056 Saint-Quentin
En Yvelines, Cedex
Tel: (33) 1 30 48 72 00
Fax: (33) 1 30 48 72 01
Korea
4th Floor KICOX Venture Center
188-5 Guro-Dong, Guro-Gu
Seoul 152-050
Korea
Tel: (82) 2 6300 4800
Fax: (82) 2 6300 4830
Germany
Waters GmbH
Helfmann-Park 10
65760 Eschborn
Germany
Tel: 49 6196 400600
Fax: 49 6196 4006010
Mexico
Waters S.A. DE C.V.
Moras No 822
Del Benito Juarez , Col Acacias
C.P. 03230, Mexico D.F.
Tel: 5255 5524 7636
Fax: 5255 5524 9376
Hungary
Waters kft
Vaci ut 202
H-1138 Budapest
Tel: 36 1 350 5086
Fax: 36 1 350 5087
Hong Kong
Waters China Ltd.
Unit 608, 6/F,
Bio-Informatics Centre,
Hong Kong Science Park
Shatin, N.T. Hong Kong, China
Tel: 852 2964 1800
Fax: 852 2549 6802
India
Waters India Pvt. Ltd.
36A, II Phase
Peenya Industrial Park
Bangalore 560058
Tel: 91 80 2 837 1900
Fax: 91 80 2 839 2157
Ireland
Waters Chromatography
Ireland Ltd., Unit 3.1
Woodford Business Park
Santry, Dublin 9, Ireland
Tel: 353 1 448 1500
Fax: 353 1 448 1510
The Netherlands
Waters Chromatography B.V.
Florijnstraat 19
Postbus 379
4870 AJ Etten-Leur
Tel: 31 76 508 7200
Fax: 31 76 508 7280
Norway
Tel: 47 63 84 60 50
Fax: 47 63 84 00 10
Poland
Waters Sp z o.o
ul. Lektykarska 25 m 21
01-687 Warszawa
Tel: (48) 22 6393000
Fax: (48) 22 6393009
Spain
Waters Cromatografa, S.A.
Ronda Can Fatj, 7-A
Parc Tecnolgic del Valls
08290 Cerdanyola del Valls
Barcelona
Direct: 902 254 254
Tel: 34 936 009 300
Fax: 34 936 009 360
Sweden
Djupdalsvgen 12-14
SE- 192 51 Sollentuna
Tel: 46 8 555 11500
Fax: 46 8 555 11550
Switzerland
Waters AG
Tfernstrasse 4
Postfach
5405 Baden-Dttwil
Tel: 41 56 676 70 00
Fax: 41 56 676 70 49
Taiwan
Waters Asia Ltd., Taiwan Branch
11F-2, No.90, Sec. 1,
Jianguo N. Rd., Zhongshan Dist.,
Taipei City 10491, Taiwan, R.O.C.
Tel: 886 2 2501 9928
Fax: 886 2 2501 9228
United Kingdom
Waters U.K. Ltd.
730-740 Centennial Court
Centennial Park, Elstree,
Hertfordshire WD6 3SZ
Tel: 44 208 238 6100
Fax: 44 208 207 7070
All Other Countries
Waters Corporation
34 Maple Street
Milford, MA 01757 USA
Tel: 508 478 2000
800 252 4752
Fax: 508 872 1990
Michael J. Yelle
Senior Director, Chemistry Commercial Operations
Waters Corporation Milford, MA, U.S.A.
www.waters.com
720003820EN
2011 / 2012
2011 Waters Corporation. Printed in the U.S.A.
Waters Chromatography Columns and Supplies Catalog
www.waters.com
2011/2012
Waters Quality Parts, Chromatography

Columns and Supplies Catalog

Catalogue Waters 2011 - 2012

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720003820EN

2011 Waters Corporation. Printed in the U.S.A.

Waters Chromatography Columns and Supplies Catalog

Waters Quality Parts, Chromatography

We are pleased to present the 2011 Waters Quality Parts,

Austria and European Export

ACQUITY UPLC Columns

Bioseparations and Analyses

Supplies and Hardware

Part Number Index

ACQUITY UPLC CSH Columns

XSelect HPLC Columns

CSH Technology is the next evolution of UPLC and HPLC particle

XSelect HPLC columns are the next evolutionary step in Waters

tec hnology. It affords outstanding batc h-to-batc h reproducibility,

LC column tec hnology. Based on Charged Surface Hybrid (CSH)

extended pH stability and seamless transferability between UPLC,

analytical HPLC and Preparative HPLC system platforms.

selectivity choices and superior peak shape improvements for basic

T he ACQUITY UPLC CSH column family provides separation scientists

compounds when operated in acidic, low-ionic strength mobile

performance in simple, LC/MS-friendly mobile phases.

Ostro Sample Preparation Products

Ostro sample preparation products provide a novel solution for the

T he Waters Positive Pressure-96 Processor offers state of the art

cleanup of phospholipids in plasma and serum. Requiring minimal to

operation for 96-well and 1 cc flangeless cartridge formats. Each

no method development and using a simple protocol, this technology

of the 96 holes in the processor is restricted in order to maintain

can be quickly implemented in order to optimize your laboratorys

workflow. Providing cleaner, more reproducible extracts t han

ACQUITY UPLC BEH200

The Protein-Pak Hi Res IEX column family consists of three ion-exchange

ACQUIT Y UPLC analytical size-exclusion c hromatography (SEC)

column chemistries each contained on monodisperse, non-porous particles

columns for the characterization of proteins and their aggregates. By

for the analysis of intact biomolecules including monoclonal antibodies,

recombinant proteins, DNA/RNA and vaccine components.

precise, high-throughput quantitative analysis of proteins suc h as

Viridis Supercritical Fluid

Online Order Center

Combining state-of-the-art media manufacturing with the industry-

Waters Online Order Center streamlines the order process by placing

leading column technology, Viridis Supercritical Fluid Chromatography

product information, availability, tec hnical specifications and price

(SFC) columns bring a new level of reproducibility to the world of

in one central location.

Combine the Online Order Center with Waters digital Chromatography

NEW ACQUITY UPLC CSH and

ACQUITY UPLC Columns Brochure

XBridge HPLC Columns Brochure

SunFire HPLC Columns Brochure

Atlantis Columns Brochure

Prep OBD Columns Brochure

NEW Viridis SFC Columns Brochure

Oasis Sample Extraction

Sample Vials and

To view and download our

Sorbent Selection Guide

Analytical Columns Wall Chart

Prep OBD Wall Chart

NEW Ostro Sample

PoraPak RXN for