LabManager November2010

November 2010
2010
Product Resource Guide
Product resource Guide

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GC SyStemS HPLC AutoSAmPLerS HPLC CoLumnS 10 HPLC SyStemS miCroSCoPeS PArtiCLe Size AnALyzerS SPeCtroPHotometerS AA SPeCtroPHotometerS Ftir SPeCtroPHotometerS rAmAn 6 10 12 14 18 20 22 24 26 28 31 34 38 41 44 46 50 54 58 62 66 68 72 74 78 82 86 89 91 94 98 100 102 106 110
Pamela Ahlberg, Editor-in-Chief
Product resource Guide 2010
ShoPPinG around Like it or not, shopping is a necessity of life (in developed countries anyway). And whether its a new car or a pair of shoes, the challenge of knowing what you want or need, finding a source, and getting the most for your money is not a simple undertaking. When it comes to purchasing sophisticated and everevolving laboratory technology, the task intensifies. The nature of your research, the amount of grant money you have or the size of your purchasing budget, and the time allotted to complete your research, are just a few factors that can influence your purchasing decision. And then there is the matter of competing vendors. Whose equipment ranks highest for doing what you need done? Which company do you currently have a relationship with? Are you getting the best information from sales people, literature and online sources? What do your colleagues recommend? And on and on. The good news is that making the best laboratory equipment purchases just got easier. What you hold in your hands represents over two years of gathering and carefully evaluating different laboratory technologies. For each of the 35 types of products included in our new Product Resource Guide, we feature a review of each that describes the state-of-the-art, comments from vendors about the evolution of the technology and what to consider when purchasing that particular piece of equipment. We have organized the product reviews according to the general categories of analytical, basic lab and life science, with products alphabetized within those categories. In addition to product reviews, we include the most up-to-date manufacturer listings along with four of the latest introductions for each product type. Please take your time reviewing our new guide, keeping in mind the products on your current to get list. And keep it handy for future purchases down the line. Our hope is that this years Product Resource Guide will be dog-eared and tattered when the 2011 edition arrives this time next year. Enjoy, and happy shopping.
00 PRODUCT FOCUS LiFe SCienCe

AutomAted Liquid HAndLinG miCroPLAte HAndLerS miCroPLAte reAderS PCr reAGentS rnAi reAGentS
SPeCtroPHotometerS uV-ViS
2010 Thermo Fisher Scientific Inc. All Rights Reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
CONTENTS
Your research demands performance labware. We deliver.

Thermo Scientific products are unmatched in quality and dependability for use in a wide range of demanding laboratory applications. Thermo Scientific Nalgene and Nunc Cryopreservation Products, Nalgene Filtration, and Nalgene Lab Quality Bottles: provide outstanding performance for consistent research results. Go to VWR International for all of your quality Nalgene and Nunc products. For special offers visit www.vwrsp.com/performancelabware
PRODUCT FOCUS baSiC Lab

AnALytiCAL BALAnCeS BioLoGiCAL SAFety CABinetS BioLoGiCAL SHAkerS & StirrerS CentriFuGeS CHiLLerS CLeAn room FurniSHinGS Fume HoodS GLoVe BoxeS inCuBAtorS LimS Low-temPerAture FreezerS miLLS And GrinderS oVenS PiPetteS reFriGerAtorS/FreezerS SPeCiALty GASeS toC AnALyzerS VACuum PumPS wASHerS wAter PuriFiCAtion SyStemS
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Thermo Scientific Nalgene cryo vials deliver superior sample protection.
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Product Resource Guide 2010
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anaLyTiCaL
gC SySTeMS
Metrohm is a lab-favorite for titration and now for ion chromatography, too!
the metrohm brand is synonymous with precision titrators that are as rugged as they are accurate. theyve been lab favorites for more than 60 years because of our reputation for stretching the boundaries of technology coupled by the pride we take in designing and manufacturing our systems. Were less known for our Ion Chromatography systems, but were here to change that.
PRODUCTS in aCTiOn
MainSTay OF ORganiC CheMiCaL anaLySiS

GC was once commonly called GLC, where the L stands for liquid. Inside GC columns are particles of a ceramic or inert material coated with a viscous liquid stationary phase that interacts with the analyte. By contrast, HPLC stationary phases are bonded to the base material. New GCs are sold with software that integrates peaks, stores methods, assists in report writing, and controls instrument functions. GC detectors have been evolving rapidly to provide greater sensitivity. Flame ionization detectors (FIDs) have been the most widely used, as they detect any molecule containing carbon. Numerous other detector types have been introduced over the years, but the most interesting is the mass detector, which is essentially a miniaturized mass spectrometer. Mass detectors provide unequivocal identification of peaks emerging from the chromatograph based on the molecules molecular weights and fragmentation patterns. High-throughput analysis was once associated with commercial labs, but today even academic groups value productivity, says Jim Edwards, business development manager at Thermo Fisher Scientific (Austin, TX). Instruments that perform faster separations are available, but this has introduced a detection bottleneck. Vendors who place a premium on accelerating chromatography should similarly speed up detection to acquire data at a speed appropriate to good precision and performance. Less easily achieved is the design of instruments that do not suffer from fatigue effects, that is, show signs of slowing down or require maintenance after one or two thousand cycles. Maintenance downtime, Edwards observes, is a productivity killer that easily negates the benefits of more rapid analysis or cycling. rapidly by fast-moving, non-recirculated air. Next, the company went after autosampling by implementing lookahead functions. It takes time to inject, clean the needle, and load and unload the sample, Baldi says. In an optimal configuration, the autosampler engages not at the precise moment it is needed, but when the oven is almost at the right temperature. A third approach is to integrate the GC with sample prep devices to provide greater flexibility and less of a hardwired configuration. PerkinElmer has recently collaborated with Tekmar (Mason, OH) on purge-and-trap sample concentration and with CTC (Zwingen, Switzerland) on solid-phase microextraction. Baldi says the goal is to minimize sample preparation. Reductions in per-injection cycle times may be achieved through the use of flow-splitting techniques that divert eluent to multiple columns or post column to one of several detectors. Splitting allows analysts to switch columns or detectors on the fly without having to turn off the instrument, allow components to cool down, and swap them out. In essence, splitting creates multiple chromatographs from one instrument.
6555 Pelican Creek Circle Riverview, FL 33578 866-METROHM (638-7646) www.metrohmusa.com
we are not newcomers to iCnot by a longshot. weve been advancing the technology and capabilities of trace-level analysis for 25 years, we just havent been very vocal about it. until now. And well start by saying the qualities that have made us a trusted brand in titration are what youll find in our ion chromatography: innovation, accuracy, precision, built-in intelligence that makes our systems so user-friendly, ruggedness. And so critical in todays lab, where every budget dollar is stretched to its limitan eye for saving you as much money as possible. were not a publicly traded company that answers to investors first. metrohm is actually a foundation that gives back to the people and city of where it was founded in Switzerland. our philanthropic roots run deep and branch out to our customers: if we do right by you, then we will be successful. it has been our time-tested recipe and one we have no intention of changing.
workflow. manual sample preparation tasks are now inline and hands-free. Good-bye expensive syringe filters/caps! Hello higher accuracy, lab efficiency and tons of money saved! our system components are manufactured and assembled by metrohm employees at metrohmwe dont cut corners. Perhaps that is why we can confidently back our systems with industry-best warranties: 3 years for the entire system, and 10 years for the suppressor. unlike other brands, we also guarantee a decade of spare parts for your system. Hows that for peace of mind? we also support you with application development and troubleshooting as well as the best maintenance services you can ask for. trust our service professionals (also metrohm employees, never outsourced!) to care for your system as if it was their ownbecause it is! these are just a few of the reasons to check out metrohm ion Chromatography systems. were just sorry we didnt do a better job of communicating them for all these years. So if you didnt think there were options when it came to iC systems, there are. And theyre great. Skeptical? thats ok, you dont just have to take our word for ithear from your peers at our special website: www.iC-changeisgood.com
GC detectors have been evolving rapidly to provide greater sensitivity.

GC systems have become faster and more selective to the point where analysts now look to dead times during analytical runs to eliminate inefficiencies. Alessandro Baldi, business manager for chromatography software at PerkinElmer (Waltham, MA), says this is best achieved by avoiding changes that will disrupt workflows or force analysts to alter established methods. Oven equilibration is one obvious bottleneck. PerkinElmer tackled equilibration by designing an oven with very low mass that cools down
Our IC systems save in so many ways

time- and cost-saving features abound. they range from a Suppressor that is guaranteed to last 10 years without compromising your results. 10 years! our systems and accessories are inherently smart, too. For example, microchips in our columns track usage and communicate optimal system parameters to improve
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anaLyTiCaL
gC SySTeMS
ReCenTLy ReLeaSeD gC SySTeMS GC-2010 Plus Capillary Clarus 680 GC
Features third-generation Advanced Flow Controllers (AFC) for high-speed analysis at a maximum pressure of 970 kPa and 1,200 mL Enables a rapid cooling time of 450C to 50C in 3.4 minutes Flame ionization detector (FID) ensures a minimum detected quantity of 1.5 pgC/s
Features twin-walled oven design with concentric air exhaust for greater separation Features oven cool-down from 450C to 50C in less than 2 minutes Includes 108 vial liquid autosampler for flexibility and automation Includes programmable pneumatic control (PPC) for added automation
Shimadzu Scientific Instruments www.ssi.shimadzu.com
PerkinElmer www.perkinelmer.com
I-Graph X Portable

No external carrier gas supply required Integrated vacuum pump allows for measurement of non-pressurized samples Features active column cooling, allowing for a lower starting temperature Analysis time ranges from 30 seconds to 3 minutes
Features a low-maintenance, consumable-free thermal modulator Offers a wide dynamic range (4 orders of magnitude) Includes a secondary oven for enhanced selectivity Cryo-focusing prior to release on secondary column provides up to a tenfold increase in analyte detectability
Pegasus 4D
LECO Corporation www.leco.com Inrag www.inrag.ch
Gc SYSTeM ManufacTurerS
Agilent Technologies Buck Scientific Dionex EST Analytical GOW-MAC JEOL USA LECO OI Analytical Parker Balston www.agilent.com www.bucksci.com www.dionex.com www.estanalytical.com www.gow-mac.com www.jeol.com www.leco.com www.oico.com www.labgasgenerators.com PerkinElmer Shimadzu SRI Instruments Teledyne ISCO Thermo Fisher Scientific TORION Technologies Varian Waters Zoex www.perkinelmer.com www.ssi.shimadzu.com www.srigc.com www.isco.com www.thermo.com www.torion.com www.varianinc.com www.waters.com www.zoex.com
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anaLyTiCaL
hPLC aUTOSaMPLeRS
ReCenTLy ReLeaSeD hPLC aUTOSaMPLeRS Model AS-2059 1260 Infinity
bOOST PReCiSiOn, RePRODUCibiLiTy FOR CRiTiCaL LC MeThODS

Chromatographers who learned their craft twenty years ago may not have been familiar with autosamplers then, but today nearly every high performance liquid chromatography (HPLC) instrument includes an autosampler as standard equipment. Autosamplers existed in the early days of HPLC but many scientists preferred manual injection, notes Simon Robinson, HPLC product manager at Shimadzu Scientific Instruments (Columbia, MD). When sample was plentiful, manual injection was a convenient way to flush the injection loop between runs, an operation most easily accomplished (at the time) by hand. from uninteresting, repetitive work. Even more time is saved when they are coupled with robotic sample preparation. Their major contributions to HPLC are reproducibility, repeatability, precision, and accuracy in delivering precise injection volumes. So who would not routinely use an autosampler? According to Robinson, academic labs still rely heavily on manual sampling and injection because their primary objective, aside from data quality, is cost-effectiveness. Another area where autosamplers are often impractical, observes Helmut Schulenberg-Schell, Ph.D., worldwide LC marketing manager for Agilent Technologies (Waldmobile phases or analyzing data. Speed, throughput, and reduced sample volumes are critical for most analytical laboratories, and autosamplers play prominently among the various fast techniques adopted toward achieving those goals. Autosamplers have become critical enablerslike columns, pumps, and detectorsof these trends, and in doing their part they have required substantial re-engineering for both stand-alone performance and integration. As such, autosamplers have been a primary target of engineering efforts to shorten cycle times vs. simply run times. After all, an HPLC or UPLC system does not add much in the way of throughput or speed in reducing analysis time if the slowest link in the cycle still requires several minutes to re-equilibrate. The wider adoption of UHPLC underscores the need for speed and the role of autosamplers within that scheme. Now, suddenly, says Schulenberg-Schell, you can run ten times as many samples on an HPLC instrument, and the question of automation in sampling, injection, sample prep, and sample tracking becomes more urgent.
High-speed, direct fill, fully automatic sample injection system Features up to 768 well positions (two 384-well microplates) Offers reproducibility less than 0.2% RSD for 5.1 to 100 l injection Less than 0.002% sample contamination for 10 l injections www.jascoinc.com
Designed for capillary and nanoflow LC, allowing injection of sample volumes from nL to L from well plates and vials Rheodyne valve, needle seat, loop and seat capillaries minimize dispersion Variable injection volume and flow through design prevents sample waste Features Peltier temperature control from 4 to 40C for thermally labile samples ww.agilent.com
JASCO
Agilent Technologies
SIL-20A/20AC
Features special needle treatment: masks active sites and minimizes ionic interactions One-piece injection port/needle seal at valve center eliminates sample transfer tubing Ultra-high-speed sample processing: 10 seconds for a 10 L injection 12-plate capacity ensures convenient processing of more than 1,000 samples
2707
Features high-resolution syringe control for high-precision injections Includes interchangeable fixed-volume sample loops Offers optional sample cooling for consistent results Able to use plates or vials, alone or in combination, for varied sampling formats www.waters.com
Shimadzu Scientific Instruments www.ssi.shimadzu.com
Waters
hPlc auToSaMPler ManufacTurerS

Agilent Beckman Coulter Bio-Rad Cecil Instruments CTC Analytics Dionex Eksigent Technologies EST Analytical Gilson PerkinElmer Shimadzu Scientific Instruments Teledyne Tekmar Varian Waters www.agilent.com www.beckman.com www.bio-rad.com www.cecilinstruments.com www.ctc.ch www.dionex.com www.eksigent.com www.estanalytical.com www.gilson.com www.perkinelmer.com www.ssi.shimadzu.com www.teledynetekmar.com www.varianinc.com www.waters.com
Autosamplers existed in the early days of HPLC but many scientists preferred manual injection.
But the main reason most lab workers preferred manual injection was that early autosamplers were not very reliable. Robinson recalls horror stories where analysts set up a tray of twenty labile samples or so, then returned the next day to find the instrument stuck on sample number two and all the remaining samples wrecked. However, Theres been so much engineering focused on improving these instruments that its safe to say autosamplers are 100 percent reliable, Robinson adds. Autosamplers spare lab analysts bronn, Germany), is in preparative HPLC. Prep work tends to be specialized, one-off, non-routine, and does not require the sensitivity or accuracy of analytical HPLC. Autosamplers provide a level of automation, throughput, and productivity that is multiplicative. Using an autosampler, one chromatographer can operate multiple HPLC systems simultaneously, injecting many hundreds of samples after spending just a few minutes with the control software. This frees workers to perform other tasks like making
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hPLC COLUMnS
ReCenTLy ReLeaSeD hPLC COLUMnS
FOR hPLC, WheRe The aCTiOn iS

High-performance liquid chromatography (HPLC) columns are considered the heart of the instrument because that is where the separations occur. Columns consist of stainless steel tubes with inlet and outlet openings. Plastic or glass may also be used, but steel supplies the highest mechanical strength. Conventional columns are filled with porous particles coated with a polymeric material that interacts with the injected sample. As particles decrease in size from conventional 10-, 7-, 5-, and 3-micron nominal diameters, the backpressure buildup increases exponentially. Thus, a column using 3-micron particles is about twice as efficient as a 5-micron column, but attendant pressures are three times as high. Systems employing particle sizes below 2 microns are referred to as UHPLC (ultra-HPLC), one of the most significant trends in HPLC column technology. UHPLC has prompted a move toward ultra-low metal content, highperformance, spherical particles and a general trend toward lower particle size columns, as well as significant improvements in HPLC performance, according to some experts. As a result, users can expect shorter run times, cleaner separations, sharper/taller peaks, and improved detection limits. Yet, UHPLC is not without its drawbacks. In addition to the higher cost, methods developed with conventional-sized particle columns do not transfer to sub-2-micron or UHPLC format. For these reasons, hints Michael McGinley, bio-separations product manager at Phenomenex (Torrance, CA), pharmaceutical companies with hundreds of instruments running validated methods have been reluctant to jump onto the sub-2micron bandwagon. Advocates of new fused-core column technology claim it provides all the performance of sub-2-micron particles but at normal pressures. Fused core employs a solid silica particle covered with a layer of porous silica, which is then infused with the bonded phase. This has the effect of shortening the path length of solutes into and out of the particles and decreasing backpressure relative to UHPLC while providing performance equivalent in many cases to UHPLC, McGinley says. higher-pressure columns, because the distinction between UHPLC and HPLC has been blurring. Since high-pressure instruments work with both conventional and UHPLC columns, users might prefer the instrument with greater capability even if they dont yet need its higher-end performance. Some vendors have replaced older HPLC systems with those that can handle both conventional columns and ones that generate high backpressures. Everyone will soon have higher backpressure capability, McGinley says. Fused-core particles, says Wayne Way, marketing manager at SigmaAldrich (Bellefonte, PA), produce more rugged columns, particularly when compared with sub-2-micron technology. Theyre great for openaccess instruments... and provide an
Hypersil Gold
Aids in the analysis of compounds that are usually difficult to retain using conventional C18 columns Available in 1.9 m, 3 m and 5 m particle sizes Highly organic mobile phase contains low salt levels, making them ideal for electrospray mass spectroscopy Ideal for applications within drug discovery, food testing and environmental analysis Thermo Fisher Scientific www.thermoscientific.com/columns
Lux
Lux Cellulose-4 is a novel cellulose tris (4-chloro-3-methylephenylcarbamate) phase Lux Cellulose-3 uses cellulose tris (4-methylbenzoate) as the selector Deliver high loading capacity and are pressure-stable up to 300 bar Offered in 3 m and 5 m particle sizes
Phenomenex www.phenomenex.com
Provide extra resolving power for a wide variety of sample mixtures Exceptional bonded phase stability and ultra-low bleed characteristics make them suitable for LC/MS Achieves baseline resolution of all compounds in a sample when other C18 columns fail Bonded to the silica is a proprietary phase consisting of a C18 chain with integral phenyl functionality MAC-MOD www.mac-mod.com
ACE C-18-AR
1.7 m
Operate at high pH, as well as low and mid pH For use with Jasco X-LC, Agilent 1290 or Waters Acquity UPLC systems Small particles will not lose efficiency even at higher linear velocity Fully scalable with proprietary C18 analytical 3 m, 5 m and 10 m particles Fortis Technologies www.fortis-technologies.com
hPlc coluMn ManufacTurerS

AAPPteC www.aapptec.com ACt Advanced Chromatography technologies www.ace-hplc.com ASt Advanced Separation technologies (Astec) www.astecusa.com Agilent technologies www.agilent.com Analtech www.analtech.com Applied Biosystems www.appliedbiosystems.com Applied Separations www.appliedseparations.com Axxiom Chromatography www.axxiomchromatography.com Beckman Coulter www.beckman.com BioChrom Labs www.biochrom.com Bio-rad Life Science www.bio-rad.com Chiral technologies www.chiraltech.com Chromtech www.chromtech.com Column engineering www.column-engineering.com dionex www.dionex.com dychrom www.dychrom.com eprogen www.eprogen.com eSA Biosciences www.esainc.com eS industries www.esind.com Fluid management Systems www.fms-inc.com Ge Healthcare Life Sciences www.gelifesciences.com GL Sciences www.inertsil.com GddS Grace davison discovery Sciences www.discoverysciences.com Hamilton www.hamiltoncompany.com Higgins Analytical www.higanalyt.com knauer www.knauer.net macherey-nagel www.macherey-nagel.com metrohm uSA www.metrohmusa.com microSolv technology www.microsolvtech.com nacalai uSA www.nacalaiusa.com optimize technologies www.optimizetech.com Pall Corporation www.pall.com Perkinelmer www.perkinelmer.com Phenomenex www.phenomenex.com Princeton Chromatography www.pci-hplc.com restek www.restek.com S.A.S. Corporation www.sascorp.jp/english SGe Analytical www.sge.com Shodex www.shodex.net Sorbent technologies www.sorbtech.com SiliCycle www.silicycle.com Supelco/Sigma-Aldrich www.sial.com tCi America www.tciamerica.com teledyne iSCo www.isco.com thermo Fisher Scientific www.thermo.com tosoh Bioscience www.tosohbioscience.com Varian www.varianinc.com wako Laboratory Chemicals www.wakousa.com waters www.waters.com whatman inc. www.whatman.com zirChrom Separations www.zirchrom.com
Users who are planning to purchase a new system are probably better off purchasing one that can handle higher-pressure columns.
Fused core has caused potential purchasers to rethink their need for a new HPLC, McGinley adds. Now they can use the same instrument theyve had for ten years and not rock the boat. But users who are planning to purchase a new system anyway are probably better off purchasing one that can handle easy transfer of methods. Methods developed on UHPLC or conventional HPLC cannot be transferred unless the two labs have the same instrument. With fused-core particle columns, different labs need only have the same column.
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anaLyTiCaL
hPLC SySTeMS
Gilson
designed to meet the growing need for personalized solutions, Gilsons PLC 2020 Personal Purification System is a fully-functioning purification system in a compact footprint intended to support an individual researcher or a small group of chemists. the PLC 2020 is designed for all levels of purification, and offers the flexibility to perform FLASH, normal-phase and reverse-phase purification. this completely integrated purification system is compact enough to fit into most fume hoods and takes up minimal bench space compared to other purification systems. in response to the growing need for easy-to-use, self-contained systems, Gilson developed the PLC 2020 Personal Purification System to meet the demand for quick and easy purification without sacrificing accuracy.
PRODUCTS in aCTiOn
USeRS DeManD PRODUCTiviTy, QUaLiTy

Conceived as a separations tool for biomolecules, high-performance liquid chromatography (HPLC) has expanded its scope significantly into chemistry, pharmaceuticals, forensics, and organic chemical analysis. The need for speed and quality has led to faster, more efficient HPLC separations. But as the time between injection and elution shrinks to less than one minute for high-pressure UHPLC (ultra high-performance LC), cycle time becomes the principal bottleneck. Terry Adams, life science business manager at Shimadzu Scientific Instruments (Columbia, MD), cites several areas of improvement that have made a difference in throughput and quality: Easy-to-clean autosamplers reduce the time between samples Fused-core or core shell columns and media perform like sub2-micron systems but at much lower pressures Multiplexing two or more HPLC systems to a single mass detector utilizes detector downtime Walk-up access allows any lab worker to queue samples onto a communal instrument and walk away Networked instruments e-mail chromatograms or instrument service reports to scientists and technical personnel Multidimensional HPLC provides peak capacity that is the product of the capacities of both columns Increased use of derivitization for fluorescence and mass detection produces extremely clean fluorescence and mass signals, even from dirty mixtures that do not resolve well on the column Although traditional HPLC still dominates methods and new system sales, customers increasingly opt for high-pressure UHPLC systems, says Phil DeLand, pharmaceutical market manager at Dionex (Sunnyvale, CA). Many are still using 3- or 5-micron columns, but theyre hedging a bet that they may want or need the speed or resolving capabilities of UHPLC down the road. Pharmaceutical industry analysts, for example, will stick with existing HPLC methods for products or DeLand believes that UHPLC has reached a point of diminishing returns in terms of speed since cycle time often takes substantially longer than a chromatography run. Increasing pressure from 1,000 to 1,200 bar, which is substantial, may reduce elution time from two minutes to one minute forty seconds, a gain of twenty seconds. How much time does that really save you within the context of sample prep and data analysis? DeLand asks. Not much. Still, other experts feel that for very high-throughput situations, every second counts. Yet many users are eager to upgrade, which brings cost issues to the forefront. At one time, conventional wisdom dictated that purchasers budget 10 percent of system cost per year for maintenance. Users are now challenging that idea, says DeLand. Twenty years ago laboratory personnel took a hands-on approach to instrumentation. This was followed by a period when soup-to-nuts service contracts dominated. During the last five years, as sample loads have increased and staff has been cut, interest in training for in-house troubleshooting has risen, and with that, demand for more reliable HPLC systems. We have come full circle.
Middleton, WI Tel: 800-445-7661 Fax: 608-831-4451 www.gilson.com
Its Your Chemistry Make it Personal!

the PLC 2020 offers an intuitive, easy-to-use software interface that allows users to start purifying compounds within minutes. the graphical icons with drag-and-drop functionality give users the ability to adjust mobile phase conditions on the fly and see on the screen where each fraction and its corresponding tube are located on the bed. with this realtime graphical sample tracking software, users can easily monitor pressure, flow rate, and % B. the unique touchscreen monitor eliminates the need for a separate PC, saving valuable bench space. to further save on space, the upper tray of the PLC 2020 holds up to four 4 L solvent bottles, giving users the ability to have four solvent lines on Pump B. interchangeable pump heads offer the flexibility of handling flow rates from 1100 mL/ min and pressure up to 4060psi depending on the application needs. in addition, the manual sample injection process (with electronic software-controlled positions for Load and inject with graphical indicator) provides an electronic actuation resulting in higher reproducibility than a lever-based manual injection process. Sample loops are available from 20 L to 5 mL, with 10 mL loops available with adapter fittings. the bed can accommodate up to three racks with customizable racks available upon request.
the PLC 2020 offers manual control options for advancing fraction collection or diverting to waste without affecting the rest of the purification run. this allows users to interrupt the normal method operation and prime the system from the run screen. Likewise, users have the ability to modify conditions including tasks and mobile phase mid-run so that samples can be quickly collected while they are being purified. Built with convenience in mind, Gilsons PLC 2020 has three uSB ports, giving users the option to connect to a printer, keyboard, mouse, or uSB drive for convenient transfer of data. the system also features automatic stopping of the mobile phase at the end of a run with a gradual ramp- down so there are no worries about the mobile phase running dry or waste overfilling. run light indicators above the fraction tubes and racks automatically turn off, notifying the users when the system is no longer in operation. these lights will also flash when an error has occurred in the method, so users can easily see from across the room if there are any issues. To learn more visit www.gilson.com/plc2020
Many are still using 3- or 5-micron columns, but they may want or need the speed or resolving capabilities of UHPLC down the road.
pipeline molecules because those have likely already been validated on low-pressure instruments. They probably would not switch to UHPLC until a new compound comes along that has already been validated with newer methods, DeLand adds.
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hPLC SySTeMS
ReCenTLy ReLeaSeD hPLC SySTeMS 1200 Infinity Series ACQUITY UPLC H-Class
Available in three models: 1220, 1260 and 1290 1220 and 1260 LCs are standardized on 600 bar pressure and 80 Hz data acquisition speed 1260 Quaternary Bioinert LC offers highest performance in bio-analysis and bio-purification 1290 LC enables users to deploy any particle type, any column dimensions and any mobile/stationary phases Agilent Technologies www.agilent.com
Combines flexibility of ternary or quaternary solvent blending with simplicity of flow-through needle injections Quaternary solvent manager (QSM) and sample manager (SM-FTN) mimic traditional HPLC system workflows, allowing a user to make the switch to UHPLC when he/she is ready Waters www.waters.com
ExpressLC-ultra
Nexera
Designed to run 0.5 mm ID micro-columns at pressures up to 10,000 psi Allows for the use of separation columns packed with <2 m particles CCD-based detector offers higher UV sensitivity with full spectral capability Features a binary gradient pump (flow rate range 1-50 l/min) with solvent selection
Optimized to enable analysis at pressures up to 130 MPa Features a 10-second injection time and 4,600-plus sample capacity Autosampler offers fixed-loop injection for ultra-fast separation and minimized peak width Intelligent Heat Balancer (IHB) minimizes band broadening during high-temperature analysis Shimadzu Scientific Instruments www.ssi.shimadzu.com
Eksigent www.eksigent.com
hPlc SYSTeM ManufacTurerS

AB SCIEX Agilent Technologies Applied Biosystems Beckman Coulter Bio-Rad Buck Scientific Cecil Instruments Dionex D-Star Instruments Eksigent Technologies www.absciex.com www.agilent.com www.appliedbiosystems.com www.beckman.com www.bio-rad.com www.bucksci.com www.cecilinstruments.com www.dionex.com www.d-star.com www.eksigent.com ESA - A Dionex Company www.esainc.com Gilson Hitachi High Technologies America Jasco Jordi Associates PerkinElmer Shimadzu Thermo Fisher Scientific Varian Waters www.gilson.com www.hitachi-hta.com www.jascoinc.com www.jordiassoc.com www.perkinelmer.com www.ssi.shimadzu.com www.thermo.com www.varianinc.com www.waters.com
2010 Waters Corporation. Waters and NuGenesis are registered trademarks of Waters Corporation. The Science of What's Possible is a trademark of Waters Corporation.
Only Waters brings tog e the powe o Only Waters brings together the power of a Scientic Data Managem nt System with the w cien ic Data anagemen System ith the g convenience an e-La Note ook. C convenience of an e-L b Notebook Cut data processing and rep rt writing time by up to 99%. s ing and repo ime up o 9%. Reduce data approval times Reduce data approval times by up to 50 75%. And seamless connect every instrument in to eamlessly nn y t ment your lab. Buying it will be your lab. Buying it will be the st of m ny qui k decision you make Bre k through your rs man quic ons y ake. Brea g o data barriers, and view the data barriers, and view the latest case studies at water ase studies a a ers.com/sdms /sdms
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anaLyTiCaL
MiCROSCOPeS
ReCenTLy ReLeaSeD MiCROSCOPeS
exTenDing viSUaLizaTiOn inTO ThRee DiMenSiOnS

Because microscopy is limited by the physics of light collection and manipulation through lenses, it will never experience the miniaturization and integration we have observed in electronics-based instrumentation. Still, vendors are constantly extending the capabilities of lenses while integrating optics with advanced automation, image acquisition and storage. Microscopy has moved well beyond conventional visual light into fluorescence, infrared and Raman, which provide spectrum-in-pixel capabilities that transform 2-D and 3-D micrographs into multidimensional visualization tools. One of the fastest-growing applications of microscopy is live-cell microscopy, or live-cell imaging. Most major microscope manufacturers supply systems suitable for analyzing live cells as they carry out reproduction, cell cycle, ingestion, metabolism, apoptosis (cell death), secretion, signal transduction, and other essential functions. Live cell analysis requires important modifications to a microscopes sample holder, principally the ability to regulate within strict limits culture conditions outside the cells natural environment. Controlled conditions include culture media and nutrients, temperature, pH, osmolarity, and dissolved gases, and these conditions need to be maintained for the duration of the experiment up to several days. Because live cell microscopy involves trade-offs between image acquisition and cell viability, instrument speed and sensitivity are critical. Instruments need to resolve images in both time and space and do so rapidly and accurately over experiments that last several days. to another and back, to keep track of multiple cells or events, and to maintain focus. With the highspeed camera, automation permits time-lapse imaging of events occurring at multiple locations within the field. Tying everything together is software that controls movement
Axio Imager 2
Light manager can store and reproduce illumination intensities for each magnification Motorized stands allow the user to select stored contrasting techniques via contrast manager Multi-user capabilities can store vital system parameters for up to ten users Permits homogeneous illumination at low magnifications Carl Zeiss www.zeiss.com/micro
Handheld Digital USB
Plugs into USB port of PC or laptop; images can be viewed at up to 500x magnification Active Pixel Technology produces crisp, sharp images and accurate color reproduction Features six built-in lights with adjustable brightness A model is available with a polarizer for reducing glare on reflective items Cole-Parmer www.coleparmer.com
ClairScope
The trend toward complete solutions is unmistakable in microscopes, as it is in other instrument markets.
The heart of a live-cell imaging system is a confocal, inverted microscope. Confocal instruments provide high-resolution 3-D images by eliminating out-of-focus light. Inverted microscopes view the sample from below instead of from above. Cells are denser than water and tend to sink in an aqueous sample, says Anthony Santerelli, advanced fluorescence product manager at Leica Microsystems (Bannockburn, IL). Inverting the microscope therefore shortens the distance between the objective lens and the cell(s) under examination. Other key components include a light source; a fast, high-resolution, low-noise camera; an imaging system; and automation. Camera speed is critical for capturing transient or short-lived events deep within cells. Automation enables investigators to move rapidly from one object of the stage, acquires data, and performs calculations. Most users interested in live-cell microscopy purchase complete systems rather than a collection of components. This will continue as biology, rather than microscopy, becomes the dominant competency among individuals performing livecell analysis. You still have some tinkerers who purchase microscopes and build systems for their unique needs, Santerelli notes. Many high-end microscopes suitable for cell imaging are, in fact, interoperable with third-party components and software. However, the trend toward complete solutions is unmistakable in microscopes, as it is in other instrument markets.
Combines a light microscope (LM) and atmospheric scanning electron microscope (ASEM) to reduce sample prep time and allow dynamic observation of real-time processes Wide-field LM is co-axially aligned with inverted electron column, making it easy to switch between LM and ASEM without moving specimen dish
Helios NanoLab 50 Series DualBeam

Features a new, high-performance focused ion beam (FIB) Features an advanced 16-bit digital pattern generator for very fine and complex patterns to be written directly with the FIB Customized detectors let users access precise topographic, chemical or crystallographic information from the sample FEI Company www.fei.com
JEOL USA www.jeolusa.com
MicroScoPe ManufacTurerS
Carl Zeiss CRAIC Technologies Edmund Optics Hamamatsu Hirox-USA JEOL Keyence Leica Microsystems Meiji Techno America MilesCo Scientific Motic Instruments Nikon Instruments Olympus Prior Scientific Unitron www.zeiss.com www.microspectra.com www.edmundoptics.com www.sales.hamamatsu.com www.hirox-usa.com www.jeol.com www.keyence.com www.leica.com www.meijitechno.com www.professionalmicroscopes.com www.motic.com www.nikoninstruments.com www.olympusamerica.com www.prior.com www.unitronusa.com
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anaLyTiCaL
PaRTiCLe Size anaLyzeRS

ReCenTLy ReLeaSeD ParTicle Size analYzerS 1190 SpectroLNF Q200
eSSenTiaL FOR Many inDUSTRieS, PaRTiCLe Sizing COnTinUeS TO UnDeRgO innOvaTiOn

Numerous technologies have emerged for measuring particle size. Sieving and sedimentation, among the oldest methods, provide quantitative sizing from millimeters upward. Optical sizing under a microscope, where particles are visualized and counted against the backdrop of a graticule (grid of evenlyspaced horizontal and vertical lines) and counted manually, is still used for many applications. Microscope-based sizing has been semi-automated through software that counts particles either directly or from photomicrographs. The most sophisticated particle sizing techniques exploit the interaction between light, sound, or electricity and particle analytes. Electroresistive methods rely on the fact that non-electrically-conductive particles reduce the flow of electricity through a conductive fluid. The most common electroresistive particle sizing instrument is the Coulter counter, which quantifies suspended cells. Light- or laser-based techniques measure dimensions and distributions of suspended or dissolved species or particles suspended in air. Sieving is one of the oldest and least expensive particle sizing methods for particles in the size range up to about 4 mm. Sieving uses various techniques to get particles through the sizing mesh, including oscillation/shaking and sound. Particle sizers that rely on dynamic light scattering (DLS) serve a sweet spot for particle analysis, between 0.6 nm and up to about 6 microns, while laser diffraction operates optimally in the 1-10 micron range. because it does not detect the molecule or particle, but calculates its hydrodynamic radius as a function of its mobility through the solution. It measures how far the particle moves under Brownian motion, notes Dr. Bodycome. Because this effect is a function of the sixth power of the hydrodynamic radius, DLS picks up species in very low abundance provided they are much larger than the analyte. Purchase decisions for particle size analyzers are based on matching the analyte particle with instrument capabilities. Users with low- or sub-micron particles will require a light- or laser-based system, while those with larger particles can usually get by with a much less expensive sieve shaker. Quality control labs analyzing samples from large vats of material should consider purchasing separate sample prep equipment, known as a riffler, to improve the likelihood that analysis samples will be representative of the batch. Price is of course a consideration, but users might want to weigh the consequences of regrinding against instrument acquisition costs.
Incorporates three lasers for highest accuracy from 0.04 to 2,500 microns Switch between dispersion modes without having to switch hardware or realign the system Optical components are permanently mounted on a cast iron base plate to ensure system is always aligned Fully compliant with ISO 13320 and 21 CFR Part 11 standard Cilas Particle Size www.particle-size.com
Features a fast CCD camera and an ergonomic sipper design Features automatic adjustment for fluid darkness; sees through black diesellubri cating oils Able to handle fluid viscosities up to 320 ISO grade without dilution An optional Automatic Sample Processor is available that automatically runs 24 samples Spectro Inc. www.spectroinc.com
Now allows high-resolution, reproducible measurement of samples from .017 to 2000 m Adds Rosin-Rammler and Folk & Ward Phi methods to its analytical capabilities Tornado Dry Powder Dispersing System keeps samples intact Reproducibility is typically better than one percent
LS 13 320
Sysmex FPIA-3000
Provides reference method for measuring porous HPLC column materials Uses automated image analysis to characterize silica particles Features an electrical sensing zone (ESZ) to measure porous particles ESZ is the reliable method, provided the device is mass calibrated to compensate for particle porosity
DLS has a broad range for very small particles but once particles get too large, its hopeless.
Life gets more difficult at the extremes, notes Jeff Bodycome, Ph.D. of Brookhaven Instruments (Holtsville, NY). DLS has a broad range for very small particles but once particles get too large, its hopeless. If all your particles are larger than a few microns, youre better off with diffraction and, larger than that, with sieving. DLS uses light at 637 or 660 nm to measure characteristics of species that are much smaller than the wavelength. That is impossible to do with conventional microscopy, for example, whose limit is objects roughly half a wavelength in size. DLS works
Beckman Coulter www.beckmancoulter.com
Malvern www.malvern.com
ParTicle Size analYzer ManufacTurerS

Agilent Technologies Ankersmid B.V. Beckman Coulter www.agilent.com www.ankersmid.com www.coultercounter.com Horiba Instruments JM Canty Malvern Instruments Micromeritics Microtrac www.horibalab.com www.jmcanty.com www.malvern.com www.micromeritics.com www.microtrac.com
Brightwell Technologies www.brightwelltech.com Brookhaven Instruments www.bic.com CILAS Particle Size Dispersion Technology Formulaction Fritsch Hach Company www.particle-size.com www.dispersion.com www.formulaction.com www.fritsch.de www.hach.com
New Star Environmental www.newstarenvironmental.com Particle Sizing Systems www.pssnicomp.com Shimadzu Sympatec TSI www.ssi.shimadzu.com www.sympatec.com www.tsi.com
Fluid Imaging Technologies www.fluidimaging.com
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anaLyTiCaL
aa SPeCTROPhOTOMeTeRS
ReCenTLy ReLeaSeD aa SPecTroPhoToMeTerS
SenSiTive, QUanTiTaTive MeTaL anaLySiS

Atomic absorption (AA) has been known since the 19th century, but it was not until the 1950s, thanks to efforts by Alan Walsh at Australias CSIRO research center, that use of AA spectrometers became routine for metals analysis. AA measures ultraviolet light absorbed by hot, atomized metals. The absorbance wavelength is unique to every metal, but the signal intensity varies by concentration. Instrumentation is straightforward, consisting of a light source, atomizer and detector. care products, paper, materials, and other products. Chuck Schneider, business unit manager for PerkinElmers (Shelton, Conn.) inorganic analysis products, breaks AA instrumentation down into three categories: flame, graphite furnace, and dedicated analyzers. PerkinElmer further delineates these into entry-level systems and higher-end systems with more extensive automation, software, data handling, and the ability to switch back and forth from flame to graphite furnace operation. Graphite furnace AA spectrometers are significantly slower than flame-atomizer instruments, but they provide several benefits. Because they concentrate the cloud of atomized metals, graphite furnace instruments require less sample than flame AA spectrophotometers20 l vs. up to 5 ml. Sensitivity (ppb vs. ppm) is also higher in graphite furnace models. PerkinElmer has recently completed a large survey of inorganic analysis customers and found that the top two factors entering into purchase decisions are customer service (including the salespersons knowledge and service support) and ease of use and setup for hardware and software. The third factor is the vendors reputation. Price is fifth or sixth on the list, Mr. Schneider says. AA has got to be dead simple to use, because instruments are used by a lot of different people who may not have specific training in the technique. Years ago a lab might have had five people operating six or seven instruments. The number of techniques has remained the same, but the number of analysts might be down to two. Lab workers need to be generalists. With more or less the same hardware technology accessible to all manufacturers, Yong Xie, AA product manager at Aurora Biomed (Vancouver, BC), believes that some vendors err in focusing on the hardware and automation alone and not enough on the ease of usea factor noted in the PerkinElmer study. The computer industry has made huge progress in both hardware and software, he says, as have advances in automation, autosampling, and unattended operation. Since these advances wont help with detection limits or other fundamental performance factors, they are best applied to enhancing the user experience, to provide the most convenient environment for operating the instrument and achieving desired objectives.
210VGP
Features an Eberl mount monochromator and user-selectable bandwith for flexibility D2 lamp emits radiation from far-UV region (<190 nm to approximately 350 nm) Three-lamp turret has individual controls for alignment and stay warm/operating facilities for each lamp
Trace 1300
Buck Scientific www.bucksci.com
Features quick auto-switch between Flame and GF atomizers Fast wavelength scan takes 30 seconds to scan from 190-900 nm Automatic acetylene flow rate control ensures hands-free operation Features a built-in camera in the GF system for efficient method development www.aurora-instr.com
Aurora Instruments
AAnalyst 600
graphite furnace instruments require less sample than flame AA spectrophotometers.

Atomizers are traditionally hightemperature flames, but graphite furnaces and various plasma sources are also used. Light sources include hollow cathode lamps (most common) and diode lasers. Detectors are most usually photomultiplier tubes. AA could be considered for nearly any application that requires the identification and/or quantification of metals, including environmental analysis, forensics, archeology, mining, agriculture and quality control for contaminant, ingredient, or trace metal in foods, drugs, personal
Features a solid-state detector for increased analytical performance over entire AA wavelength range THGA tube design eliminates most interferences Features longitudinal Zeeman-effect correction for improved accuracy and detection levels Includes automated flame and furnace atomizer PerkinElmer www.perkinelmer.com
ContrAA 700
Combines flame, hydride and graphite furnace in one device HR-CS AAS technique closes the methodological gap between AAS and ICP OES Features High-Resolution Continuum Source Technology for trace and ultra-trace analysis using atomic absorption with a graphite furnace
Analytik Jena www.analytik-jena.com
aa SPecTroPhoToMeTer ManufacTurerS
Analytik Jena Buck Scientific GBC Scientific Hitachi High Technologies PerkinElmer Shimadzu Scientific Instruments Thermo Fisher Scientific Varian www.analytik-jena.com www.bucksci.com www.gbcscientific.com www.hitachi-hta.com www.perkinelmer.com www.ssi.shimadzu.com www.thermo.com www.varianinc.com
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anaLyTiCaL
FTiR SPeCTROPhOTOMeTeRS
ReCenTLy ReLeaSeD FTiR SPeCTROPhOTOMeTeRS
PiCking UP STeaM in nOnTRaDiTiOnaL MaRkeTS

Fourier transform infrared (FTIR) spectroscopy, a subset of infrared (IR) spectroscopy, uses a mathematical algorithm, Fourier transform, to translate raw infrared data into a spectrum. Like IR, FTIR is useful for the analysis of organic and inorganic compounds that exhibit changes in polarity as a result of the vibration, spinning, or perturbation of molecular bonds. FTIR methods are common in such industries as foods, materials, chemicals, pharmaceuticals, forensics, and others. Advantages of FTIR over conventional IR are higher resolution, better signal-to-noise, easier analysis of very small samples and poorly-absorbing species, and much more rapid analysis. Theres a lot of pressure to bring FTIR out of the lab and into the field, said Haydar Kustu, global marketing communications manager at Bruker Optics (Billerica, MA). One of the most exciting scientific advances in this regard are MEMS (microelectromechanical systems), which enable rugged, low-cost handheld devices. MEMS shrink the interferometer, Kustu adds. Another enabling technology has been quantum cascade lasers (QCLs), which are brighter and more sensitive than conventional lasers. MEMS and QCLs will open up many more niche or field applications for FTIR. Unlike laboratory instruments, field analyzers are typically dedicated for single analytes. These are built for specific purposes. You cannot swap out accessories, change from transmission to reflectance, or change the detector or the source. You cannot configure them on the go, says Kustu. so due to the economic downturn, but remain unchanged in nature over the past two decades. Another factor that still matters very much is the perception of how well vendors support their products, both around the sale and afterward. In this regard, Sellors suggests that global communication within and among
Can be operated without any scheduled maintenance for ten years Features a simple and robust interface for data acquisition and routine analysis Universal sampling compartment accommodates accessories like liquid vial holders and probes Includes a user-friendly software interface enabling operations in a 21 CFR Part 11-compliant mode ABB www.abb.com
MB3600-PH
ML with DialPath Technology
FTIR-8400S
Three selectable pathlengths from 30 to 250 microns are always available and can be switched in seconds Eliminates traditional liquids cells which are prone to leaking, fringing and breakage Entire system fits on a benchtop in a space no greater than 8 square inches Measures liquid samples so quickly that autosamplers may be eliminated A2 Technologies www.a2technologies.com
Theres a lot of pressure to bring FTIR out of the lab and into the field.
The proliferation of FTIR into materials, fuels, biology, environmental testing, and homeland security raises issues of usability and user-friendliness that did not exist a decade ago. Users today are more likely to be non-specialists or occasional users than IR spectroscopists, says Jerry Sellors, Ph.D., manager for FTIR at PerkinElmer (Beaconsfield, UK). In this environment, instrument makers must emphasize user-friendliness for both hardware and software. Users today are less interested in buying an FTIR spectrophotometer than they are in acquiring a biodiesel or contaminant analyzer. Purchase decisions are influenced more by how well an instrument performs a specific task than by its technical specifications. Cost and the performance/price ratio remain factors, perhaps more companies helps spread the word about which instrument manufacturers make the cut. In addition to budget issues, purchasers need to be clear, before they buy an FTIR spectrometer, on their applications, advises Dr. Smith. Its too easy to get taken in by the gee whiz factor. Most vendors offer quality instruments; the difference for the average user may be the software. Its imperative to take the software for a test drive, and not just let the salesman show you how it works. Take a spectrum yourself before you buy, and get references [from people] who can vouch for the manufacturers service.
Dynamic Alignment system maintains optimal alignment during data acquisition Features a peak-to-peak signal-to-noise ratio of 20,000:1 Quick-start function prompts user through every action from setting scan parameters and acquiring spectrum to detecting peaks and printing Shimadzu Scientific Instruments www.ssi.shimadzu.com
Exoscan and FlexScan

Battery-operated, handheld spectrometers for field applications Identify minerals and soil components and quantify molecular compounds including oil contamination or degree of hydration in soil Feature a diffuse reflectance sampling interface A2 Technologies
www.a2technologies.com
fTir SPecTroPhoToMeTer ManufacTurerS

A2 Technologies ABB Bruker Optics JASCO Newport Corporation PerkinElmer Shimadzu Scientific Instruments Varian www.a2technologies.com www.abb.com www.brukeroptics.com www.jascoinc.com www.newport.com www.perkinelmer.com www.ssi.shimadzu.com www.varianinc.com
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anaLyTiCaL
RaMan SPeCTROPhOTOMeTeRS
ReCenTLy ReLeaSeD RaMan SPeCTROPhOTOMeTeRS
STePChiLD OF vibRaTiOnaL SPeCTROSCOPy COMeS OF age

Raman has become the go-to analytical method in materials science, pharmaceuticals, and homeland security. Its advantages are that it is nondestructive, detects analytes through packaging, and requires no sample preparation. The principal drawback is that Raman provides only confirmation, not quantitation. Richard Larsen, Ph.D., spectroscopy products manager at Jasco (Easton, MD), divides Raman instrumentation into three categories: macro, micro, and imaging. Macro measures bulk properties in traditional spectrophotometer mode, micro instruments incorporate microscopy to analyze particles or very small samples, and imaging Raman involves spectral analysis of materials in 2D and 3D mode. Higher-end Raman spectrometers provide exquisite spatial and spectral resolution. sives. What makes handheld instruments possible are inexpensive 785 nm diode lasers that consume less than 1 amp. Most users of Raman today view it as a tool like every other lab instrument. To make systems appeal to these customers, vendors have to think a lot more about making self-optimizing instruments that are easy to operate, with little or no learning curve, says Joe Hodkiewicz, Raman product manager at Thermo Fisher Scientific (Madison, WI). This can be a challenge to designers with a lot of Raman experience, to whom instrumentation is second nature. Telling them they have to simplify things is a challenge. Hodkiewicz compares the hurdles to making Raman accessible with the rise of digital cameras that allow users to take professional-quality photographs pated applications that may require future upgrades, for example, the use of different excitation lasers, microscope and automation options, specialized sample cells, and remote fiber probes. Larsen views Raman as being at approximately the same level of acceptance and use as FTIR was 20 years ago. Back then an FTIR cost $100,000 to $200,000. Now were down to sub- $20,000 FTIRs. Raman is going in the same direction. I dont think it will ever be as widely used as FTIR, but theres no doubt there is still plenty of room for growth. Raman has traditionally been viewed as an exotic, if not problematic, analysis method. The main limitation, says Haydar Kustu, global marketing communications manager at Bruker Optics (Billerica, MA), was the laser which was difficult to operate. Today, manufacturers are improving their Raman spectrometers by paying more attention to the laser. This has led, he says, to greater adoption of Raman in labs, and notable handheld product introductions as well. The signature trait of these newer systems has been reliability and ease of use.
RamSpec 1064-HR
Features Volume Phase Grating (VPG) as the special dispersion element and InGaAs array detector as the detection element Helps reduce the likelihood of human error by eliminating sample preparation and test interpretation Uses a fiber optic bundle or slit optics arrangement based on customer preferences Features self-automated wavelength calibration BaySpec www.bayspec.com
RAMANRXN2 1000
Provides the fluorescence rejection of a FT-Raman spectrometer and ease of fibre-optic interfacing of a dispersive Raman analyzer Provides an effective route for in situ monitoring Offered with either iC Raman 4.1 for reaction development and understand, or synTQLite for process monitoring and control Kaiser Optical Systems www.kosi.com
Advantage 1064
NRS-5000/7000
InGaAs photocathode and electron bombardment CCD technology provide high sensitivity Features a spectral range of 200 to 2,200 cm-1 and a resolution of 10 cm-1 Includes a sample cell attachment for 8 mm vials, NMR tubes or MP tubes DeltaNu www.deltanu.com
Capable of integrating as many as 8 excitation lasers from the UV to the NIR Offers high-speed imaging using the Software Programmable Raman Integration System (SPRIntS) Verti-Scan ensures consistent confocal sample excitation for undistorted 3D images Dual Spatial Filtering reduces sample fluorescence while enhancing spatial resolution www.jascoinc.com
JASCO
raMan SPecTroPhoToMeTer ManufacTurerS

Agilent Technologies B&W Tek BaySpec Bruker Optics DeltaNu Enwave Optronics HORIBA Jobin Yvon JASCO Malvern Instruments Ocean Optics PerkinElmer Renishaw Shimadzu Scientific Instruments Thermo Fisher Scientific Varian www.agilent.com www.bwtek.com www.bayspec.com www.brukeroptics.com www.deltanu.com www.enwaveopt.com www.jobinyvon.com www.jascoinc.com www.malvern.com www.oceanoptics.com www.perkinelmer.com www.renishaw.com www.ssi.shimadzu.com www.thermo.com www.varianinc.com
Manufacturers are improving their Raman spectrometers by paying more attention to the laser.
Portable Raman spectrophotometers come in plug-in and batterypowered formats. The latter, which are mostly handheld field analyzers, are popular for inspecting materials on loading docks, in oil exploration, for crime scene analytics, and for detection of narcotics and explowithout worrying about exposure times, apertures, and film speed. Thats the approach were taking with spectroscopy, he says. Another trend is the desire for flexibility and expandability. Users, Hodkiewicz says, are purchasing not just for today but for antici-
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Uv-viS SPeCTROPhOTOMeTeRS
BioTeks new Epoch Multi-Volume Spectrophotometer System
Drenkow, UV-Vis product manager at Agilent (Loveland, CO). Other factors entering into purchase decisions are cost, throughput, and the reliability of data. Sample size is an ongoing issue in analytical science. People want more from less, notes Philippe Desjardins, scientific marketing manager at NanoDrop (Wilmington, DE). From this need has emerged microvolume UV-Vis, which analyzes liquid samples in the 0.5 to 50 l range. Several vendors offer microvolume-only instruments or microvolume cells for conventional UV-Vis spectrophotometers. In its most elegant configuration, a microdrop of sample is deposited atop a small pedestal and a second pedestal immediately approaches to complete the optical path by contacting the samples meniscus. Embedded in each pedestal is an optical fiberone serving as a conduit for the light source, the other for the detector. The sample stays in place due to surface tension, but the path length may be optimized by adjusting the distance between 0.05 mm and 1 mm, depending on the sample concentration. This eliminates the need, as in conventional UV-Vis, to remove the cuvette and dilute or concentrate the sample.
Bioteks new epoch multi-Volume Spectrophotometer System combines two innovative products into system designed for a wide range of applications, from nucleic acid and protein quantification on a micro scale to cell-based assays in microplates, BioCells or standard cuvettes. the epoch microplate Spectrophotometer offers a 200 nm to 999 nm wavelength range without interference filters for measurements in 6 to 384 well microplates, while the
PRODUCTS in aCTiOn
iTS aLL in The ChROMOPhOReS

Ultraviolet-visible spectroscopy (UV-Vis) measures and identifies chemicals that absorb in the ultraviolet and visible regions of the electromagnetic spectrum. UV wavelengths range from 10 to 400 nanometers, while the visible spectrumcolors visible to the human eyelie from approximately 400 to 800 nanometers. UV-Vis can be used to identify the presence of chemicals and their concentrations. Its a popular technique in chemistry, foods, pigments, pharmaceuticals, polymers, and the life sciences. Compounds analyzed by UVVis possess chromophores, or color-absorbing carbon-carbon, carbon-oxygen, or carbonnitrogen double bonds, as well as carbon-halogen single bonds. Depending on the extent and type of unsaturation, particularly whether the bonds are conjugated (alternate with single bonds), compounds may absorb in the UV or visible region of the spectrum. Chemicals such as carotene and certain dyes, which have extensive conjugation, are brightly colored and absorb in the visible region. Conjugation causes a shift in absorbance to higher wavelengths. At one time, vendors routinely offered separate instruments for UV and Vis, but today the two tend to be combined. Some manufacturers add a third capability, near-infrared (NIR), which is immediately above the visible region. UV-Vis spectrometers come in four basic models: low-cost singlebeam, dual-beam, array-based, and handheld. Single-beam techniques must apply a correction for the loss of light intensity as the beam passes through the solvent. Dualbeam spectrometers use a second solvent reference cell and perform the correction automatically.
P.O. Box 998, Highland Park Winooski, VT 05404 TEL: 888-451-517 www.biotek.com
take3 Plate extends the systems applications to very low volume nucleic acid and protein quantification. up to sixteen 2 ul dnA, rnA or protein samples can be quickly quantified saving valuable time and samples. this system is well suited to the budget sensitive laboratory that needs maximum functionality and application flexibility. the epoch microplate Spectrophotometer features a monochromator based optical system, with a wide 200 to 999 nm wavelength range, selectable in 1 nm increments. the main benefit is the ability to run a multitude of biomolecular assays without needing to purchase application specific filters. Controlled by the powerful Gen5 data Analysis software, users need only to select the desired wavelength and read the microplate. when the assay wavelength is unknown, a spectral scan can be quickly run to determine the absorbance peak. epoch can measure 6 to 384 well microplates with either a single data point taken in the center of the well, or by well area scanning in larger well diameters to provide multiple measurements that can be analyzed more closely. measurements made in microplates can be corrected for pathlength by Gen5s automated pathlength correction option, which is an especially useful tool for direct quantification assays. when used with the epoch microplate reader, the take3 multi-Volume Plate extends applications
users love capturing the entire spectrum in seconds.

Single- and dual-beam benchtop instruments use a broad-spectrum lamp as the light source, and most use a photomultiplier tube as the detector. Some instruments employ photodiode array detectors. Handhelds employ single-wavelength light-emitting diode light sources and photodiode detectors. UV-Vis instruments have become much faster, more compact, and feature-laden. Array instruments, in particular, provide a level of detail and throughput that was previously unavailable. Although they may have originally been interested in just one or two wavelengths, users love capturing the entire spectrum in seconds, says Grant
into the increasingly important low volume area. take3 allows measurement of up to sixteen 2 L samples at a time so that dnA, rnA or protein samples can be measured quickly and without diluting. the take3 plate uses a custom designed fused silica slides, one of which is precision printed with sixteen 2 mm microspots the ideal size for samples as low as 2 L. when the top of the take3 is closed, a nominal 0.5 mm pathlength is created so that sample concentrations can be quickly and accurately made with results produced automatically, via pre-programmed applications within the custom Gen5 take3 module.
together, epoch and take3 comprise a system that allows the laboratory an assay multi-tasking capability in one compact footprint at a fraction of the cost of typical instrumentation and in a fraction of the time that would be required to accomplish the same results.
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anaLyTiCaL
Uv-viS SPeCTROPhOTOMeTeRS
LiFe SCienCe
aUTOMaTeD LiQUiD hanDLing
ReCenTLy ReLeaSeD Uv-viS SPeCTROPhOTOMeTeRS 8453 GENESYS 10S and BioMate 3S

GENESYS instruments are capable of producing scan data at speeds up to 4,200 nm per minute, and feature a unique optical design that reduces stray light No warm-up time is required with the BioMate 3S, and samples are not constantly exposed to UV light BioMate 3S includes pre-configured test methods for nucleic acid, protein and cell growth measurements Thermo Fisher Scientific www.thermoscientific.com
Photodiode array (PDA) light path scans a complete spectrum from 190 to 1,100 nm in less than one second Fewer moving components reduce the need for recalibration Uses a deuterium discharge lamp for the full UV and visible range
enabLeRS OF high-ThROUghPUT aSSayS

Automated liquid handlers encompass a range of instruments and systems whose function is to dispense liquids rapidly, usually in very small quantities, at user-specified volumes, and with great accuracy, precision, and reproducibility. As the successors to manual pipettes, automated liquid handlers are the principal enablers of rapid experiments and assays conducted in tubes, vials, or microtiter plates. Liquid handlers are often just one component of systems consisting of microplate handlers, washers, readers, stackers, shakers, and incubators. Automation became necessary as assays were miniaturized from vials to tubes, and finally to microplates, and as researchers ibility, but not necessarily high throughput, are desired. Any time you work with many samples and small quantities of fluids, automating liquid handling with a workstation will provide good return on investment, says Scott Eaton, director of robotics marketing at Hamilton (Reno, NV). Another factor to consider, Eaton says, is the effect of physical forces on very small liquid-dispensing volumes used in higher density plates. While 96-well plates remain the most common, 384- and even 1,586- well systems that employ sub-microliter volumes are gaining in popularity. At these volumes, evaporation and absorption onto the plastic plate surface become issues. dispensing. In the past, liquid handlers performed just one function; today, they are application solutions in which liquid handling is part of a larger picture, Hall says. Hall suggests that potential buyers analyze their liquid-handling needs the way a cook examines a recipe. What are the ingredients? What labware are we dispensing from and into? What volumes are involved, and what sample-tip options are available? Hall says. Users who fail to optimize the liquid handlers fluidics design to desired volumes will be forced to compromise either on performance or throughput. Liquid handling is just one component of what may be a complex workflow, according to Jason Greene, liquid handling product manager at BioTek (Winooski, VT). Operating manually, users must work through the various reagent additions, incubations, washing, and reading steps, he says. Nobody likes to wash microplates. Its pretty easy to get users to buy into the idea of automation on that function alone.
Agilent Technologies www.agilent.com
BioSpec-nano
Libra Series
Ideal for quantitation of nucleic acids or fluorescent-labeled nucleic acids and protein analysis Sample mounting, measurement and cleaning are all performed by the device Analysis can be performed with 1 l or 2 l samples Analysis results can be converted to PDF or CSV files Shimadzu www.shimadzu.com
Feature a color touch screen, USB connection, optional Bluetooth for data transfer and an optional built-in printer Includes application-based software for all stand-alone and PC-controlled instruments Equation Editor allows users to program specific methods that include calculations based on measured data
Biochrom
www.biochrom.co.uk
uV-ViS SPecTroPhoToMeTer ManufacTurerS

Agilent Technologies Analytik Jena Beckman Coulter www.agilent.com www.analytik-jena.com www.beckman.com www.biotek.com Buck Scientific Cecil Instruments GBC Scientific Hach Company Hitachi Ocean Optics Shimadzu Scientific Instruments S.I. Photonics Thermo Fisher Scientific Varian www.bucksci.com www.cecilinstruments.com www.gbcscientific.com www.hach.com www.hitachi-hta.com www.oceanoptics.com www.ssi.shimadzu.com www.si-photonics.com www.thermoscientific.com www.varianinc.com
Get a better reaction
In the past, liquid handlers performed just one function; today, they are application solutions.
switched from radionuclide-based assays to tests that used nonradioactive detection. Biology, medical testing, and screening of development-stage drugs are the primary markets for automated liquid handling. The energy, environmental, and heavy industries also use liquid handlers when accuracy and reproducAutomated liquid handlers have evolved from automated pipetting systems to workstations that employ liquid handling as one component, according to Nance Hall, vice president for automation and detection systems at PerkinElmer (Waltham, MA). Todays systems perform washing, incubation, and plate manipulation in addition to
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HaMIlTOn
nimBuS is Hamiltons compact automated pipetting workstation, offering a high-density deck in a small footprint. Available in 4-independent channel or 96-multi-channel platforms, nimBuS provides speed, flexibility, and high performance air-displacement pipetting at an affordable price, even for small budget-challenged labs. An optional gripper with extended reach allows for easy labware transport and seamless integration to peripheral devices. the fastest selling platform 5 years running, Hamiltons StAr line of automated liquid handlers offers world-class performance and reliability for all your assay and sample preparation needs. our flagship platform features up to 16 independent pipetting channels as well as 96 or 384 multi-channel heads, providing the flexibility and throughput needed in todays busy lab.
PRODUCTS in aCTiOn
ReCenTLy ReLeaSeD auToMaTed liquid handlerS STAR Series Sciclone G3
Feature up to 16 independent pipetting channels as well as 96 or 384 multi-channel heads Autoload option provides barcode tracking of samples, labware, racks and carriers Pipetting channels and labware grippers move independently of each other, supporting a wide range of labware Based on air displacement pipetting technology Hamilton Robotics www.hamiltonrobotics.com
Easily manages and processes 96 blood tubes with an innovative rack system Uses on-deck sensors and integrated bar code reader to identify and track samples Features non-contact liquid level and clog detection Pipetting approach eliminates the need for electrostatic tips
4970 Energy Way, Reno, NV 89502 USA tel 775.858.3000 1.800.648.5950 fax 775.858.3024 email sales@hamiltoncompany.com www.hamiltonrobotics.com
Caliper Life Sciences www.caliperLS.com
Freedom EVO Series
Liquid volumes range from 100 nl to 5ml and can be extended with DynamicFill Technology to 50 ml and higher Pressure Monitored Pipetting (PMP) detects errors by comparing recorded and realtime-simulated pipetting pressure signals Liquid handling arm can be equipped with disposable tips and/or washable tips Tecan www.tecan.com
Picks up one column, one row or one tip without changing heads Ideal for performing serial dilutions in 384- and 1,536-well plates Employs CO-RE (Compressed O-Ring Expansion) technology and air-displacement pipetting When used with Rocket Tips, the need for a second head and arm is eliminated Hamilton Robotics www.hamiltonrobotics.com
CO-RE 384 Shifted Tip Pickup (STP)
the miCroLAB nimBuS is Hamiltons compact automated pipetting workstation, offering speed, flexibility, precise tip positioning, superior pipetting performance and affordability, all in a space-efficient footprint. Available as a 4-independent (1ml or 5ml channels) system for flexibility or a 96-multi-channel (1ml) head system for speed, both nimBuS platforms incorporate Hamiltons novel Co-re (Compressed o-ring expansion) technology, which facilitates tip and tool pick-up via a robust lock-and-key style attachment. this enables a positional precision of 0.1mm in all axes, critical for 384-well plates and applications such as mALdi target spotting which require accurate and reproducible sample placement. Co-re technology requires no vertical force for tip attachment or ejection, ensuring that potentially dangerous or contaminating aerosols are not produced upon tip ejection. this mechanism also allows tip pick-up from Hamiltons proprietary nested 96 disposable tip racks (ntrs). Space-saving ntrs may be stacked up to six high (576 tips/stack) in one deck position, thus freeing up deck space for sample plates and other labware. nimBuS utilizes the same proven air-displacement pipetting technology as found on Hamiltons flagship StArline of automated liquid handlers, so there is no sacrifice in pipetting performance (i.e. 2% CV at 10ul volumes). the nimBuS 4 and nimBuS 96 platforms incorporate capacitive Liquid Level detection (cLLd), providing real-time feedback of volumes in plates and tubes as methods are conducted.
auToMaTed liquid handlinG ManufacTurerS

Agilent Technologies Apricot Designs Aurora Biomed Beckman Coulter BioMicroLab Biosero Biotage BioTek Instruments BioTX Automation Caliper Life Sciences CETAC Drummond Scientific Essen Instruments Gilson www.agilent.com www.apricotdesigns.com www.aurorabiomed.com www.beckmancoulter.com www.biomicrolab.com www.bioseroinc.com www.biotage.com www.biotek.com www.biotxautomation.com www.caliperls.com www.cetac.com www.drummondsci.com www.essen-instruments.com www.gilson.com Hamilton Robotics Hudson Robotics Jencons Scientific Labcyte Labnet International Molecular Devices PerkinElmer Staubli TECAN Thermo Scientific Tomtec TriContinent Scientific www.hamiltonrobotics.com www.hudsoncontrol.com www.jenconsusa.com www.labcyte.com www.labnetlink.com www.moleculardevices.com www.perkinelmer.com www.staubli.com www.tecan-us.com www.thermo.com/matrix www.tomtec.com www.tricontinent.com
nimBuS 4 also allows for pressure-based Liquid Level detection (pLLd), essential for nonconductive reagents and volatiles. Furthermore, air-displacement pipetting affords the use of Hamiltons proprietary monitored Air displacement (mAd) technology on nimBuS 4, which uses pressure within the channel to verify liquids have been successfully aspirated and no clots or foam have been detected. these technologies as well as Hamiltons novel Anti-drip Control (AdC) feature provide for a highly robust pipetting environment. optional total Aspiration and dispense monitoring (tAdm) software provides an even higher level of process control, recording and documenting all pipetting steps executed. Both nimBuS platforms feature an optional gripper, capable of moving plates and ntrs on or off the nimbus deck. 270 rotation and extended reach allows integration with off-deck peripheral devices. nimBuS also offers an array of on-deck integration options, including heater/shaker, vacuum station and magnetic separation devices. A cumulative blend of enabling technology, a range of integrated options, intuitive software and the backing of Hamiltons renowned service and applications support makes the nimBuS an essential tool for budget and space-constrained labs. For more information call 800.648.5950 or visit www.Hamiltonrobotics.com
Innovadyne Technologies www.innovadyne.com
Rainin Instrument Company www.rainin.com
Eppendorf North America www.eppendorfna.com
Zinsser North America www.zinsserna.com
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PRODUCTS in aCTiOn
The gLUe ThaT binDS MiCROTiTeR PLaTe-baSeD anaLySiS SySTeMS

Automated systems employing microplates in biological and chemical assays may consist of liquid dispensers, plate washers, mixers, readers, sealers, labelers, shakers, incubators and storage. Tying these components together are microplate handlers, which feature a computer-controlled robotic arm. Once programmed with a specific workflow, microplate handlers move microplates and deliver them to locations on various instruments precisely when they are needed. Microplate handlers evolved from bulky industrial robots that were programmed and adapted to hold microplatesa task for which they were clearly not designed. They were overkill for the weight of a plate, observes Todd Christian, who heads global marketing at Agilent in Palo Alto, Calif. Todays handlers are designed specifically for laboratories and, with lab space at a premium, optimize the use of vertical space, thereby taking up less room on a benchtop. Large laboratory equipment vendors may sell stand-alone robotic plate handlers, but most customers prefer purchasing the robotics already integrated with plate readers, stackers, and other instruments. Ease of use is a top priority with most purchasers of microplate handling systems. Robots must be smart about the space around them, particularly with respect to collision avoidance, and capable of rapidly learning precise endpoint positions in three-dimensional space. Most plate handlers must be taught key positions in space by manually moving the arm to the desired location and noting that in the control software. The path to the medical testing laboratories, purchase microplate handlers to carry out one or several specific tasks 24 hours a day. Others, such as academic institutions, expect flexibility, versatility, and programmability, particularly for acquiring new plate readers or workflow changes. These users are better off investing in plate-handling capabilities based on anticipated needs; for example, rapid swapping in of components, particularly readers. Understanding the customers workflow the science behind the robotics and being willing to source third-party instruments for specific microplate operations are traits that customers seek among vendors of microplate handlers. The ability to understand the experiment and to help customers select the right solution based on the right components is highly valued, says Mary Duseau, VP of global sales for molecular medicine at PerkinElmer in Waltham, Mass. She adds that due to the complexity of microplate handling systems and the general lack of experience with robotics, purchasers of the systems require more handholding than do those who buy other instruments. Its important to serve both expert and novice customers, Duseau said.
Microplate Handling
the Stakmax microplate Handling System is an integrated microplate stacker for use with molecular devices Spectramax microplate readers and Aquamax microplate washers, providing simple, powerful, walk-away benchtop automation for increased throughput. Achieving higher throughput in laboratory experiments depends on the ability to easily process batches of microplates. Historically, joining off-the-shelf components together has made for expensive, difficult-to-use, and error-prone integrations. to address these issues, molecular devices leveraged its 20 years of experience with the automation industry to develop the Stakmax microplate Handling System to provide dedicated benchtop automation for batches of up to 50 microplates, in a small footprint. integration of a Stakmax System with molecular devices microplate reader is a straightforward process and can be completed in less than 15 minutes, including alignment. integration with an Aquamax microplate washer can be completed in 5 minutes, with no alignment necessary. once integrated to a molecular devices microplate reader, the Stakmax System is operated through molecular devices industry-leading Softmax Pro data Acquisition & Analysis Software providing a common interface for operating both the reader and stacker, avoiding problems with communication hand-offs. when integrated to an Aquamax microplate washer, all programming and operation is handled by the Aquamax microplate washer programming touch screen. no external computer is necessary. http://www.moldev.com/Products/Instruments/Microplate-Handlers.html
1311 Orleans Drive, Sunnyvale, CA 94089-1136 USA Phone: 1 408 747 1700 | Fax: 1 408 747 3601 Toll Free: 1 800 635 5577 www.moleculardevices.com
[First-time users] are especially nervous that the handler will drop the plate or improperly position it.
location does not matterthe robot automatically takes the shortest route. Maneuvering around objects requires creating and storing an intermediate location. Some advanced control software packages allow users to input set points in the software without actually moving the arm. First-time users approach robotics with a combination of awe and suspicion. They are especially nervous that the handler will drop the plate or improperly position it, Christian notes. Moreover, user requirements and expectations of automation differ widely. Many organizations, such as
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CytoOne Cell Culture Performance Ware
your cells deserve the best. Consistent growth surfaces with certified testing. Premium grade, optically clear polystyrene. Class 100 production, iSo 9001 manufacturing, and rigorous quality control. your cells will perform with Cytoone and so will you. Gripping aids on plates and dishes promote secure handling. true 360 chimney wells minimize edge effects and prevent cross contamination. easy open zip top sleeves let you reseal and protect unused flasks.
PRODUCTS in aCTiOn
ReCenTLy ReLeaSeD MicroPlaTe handlerS
StakMax
Integrate with Molecular Devices microplate readers and AquaMax microplate washer Process batches of up to 50 microplates Walk-away automation Increased throughput Molecular Devices www.moldev.com
Accommodates SBS-approved plate types, including 96-, 384-, 1,536-, deepwell and automation-friendly PCR plates Features automatic reshuffle, with ability to sort and store plates in stackers for first-in, first-out (FIFO) processing Equipped for standalone operation or integration with other liquid handling devices PerkinElmer www.perkinelmer.com
PlateStak
See the complete line of Cytoone cell culture performance ware at www.usascientific.com/Cytoone. Samples available. Call us toll free at 1-800-522-8477 or email info@usascientific.com.
Features a compact footprint and removable stacks Automates single or multiple instruments Features expandable capacity for up to 320 microplates Includes a rotational gripper for portrait and landscape carrier formats BioTek www.biotek.com
BioStack Twister II
BenchCel
Cytoone plates, dishes, and flasks are available in the most commonly requested sizes and formats for both adherent and suspension cells. All are certified dnase, rnase, dnA, and pyrogen free and are gamma sterilized.
Flasks
Cytoone flasks are supplied in easy open, resealable zip top bags that protect unused flasks. Anti-tip skirts and base bars improve stability during handling. Flasks also feature molded volume graduations and frosted writing surfaces. wide, anti-drip necks provide easy access for pipets and scrapers. Cytoone is available with filter caps or two-position plug seal caps that let you choose secure manual venting or an airtight seal. Vented stacking rims aid thermal equalization. All flasks are 100% pressure tested for leak-free assurance. Crystal-clear consistent surfaces provide distortion-free microscopy.
Features an 8-second transfer time from stack to instrument Offers 2-, 4- or 6-rack options for a maximum of 360 standard microplates Delidding function removes and replaces microplate lids as necessary Able to integrate multiple instruments into a single benchtop system Agilent Technologies www.agilent.com
Multiple Well Plates

Cytoone plates offer consistent, crystal-clear surfaces for optimum growth and distortionfree microscopy. 6-, 12-, 24-, 48-, and 96-well plates have molded alphanumerics for well identification, frosted writing surfaces, and complete 360 chimney wells to minimize edge effects and prevent cross contamination. 384-well plates have molded alphanumerics and raised well rims. ridged bases secure your grip and vented skirts allow air circulation between stacked plates. non-reversible lids with condensation rings minimize contamination and are vented for improved gas exchange. unique support tabs reduce surface contact when lids are set aside or propped open.
MicroPlaTe handler ManufacTurerS

Agilent Technologies Biotage BioTek Instruments Caliper Life Sciences Molecular Devices PerkinElmer Phenix Research Products Staubli Thermo Scientific Tomtec www.agilent.com www.biotage.com www.biotek.com www.caliperls.com www.moleculardevices.com www.perkinelmer.com www.phenixresearch.com www.staubli.com www.thermo.com www.tomtec.com
Scrapers
Cytoone scrapers and lifters have flexible silicone rubber blades for excellent contact and cell removal. they are available in three sizes for cell harvesting in a variety of dishes, flasks, or plates. Pivoting blade scrapers reach into corners and have a flexible ABS handle for better contact inside flasks. the fixed blade scraper lifts cells from 6-, 12-, or 24-well plates or dishes.
Premium quality CytoOne provides excellent results and offers many user-friendly features.
Dishes
Large Cytoone dishes have ergonomically placed grip tabs for a secure grasp with less strain. Small dishes have beveled outer edges to avoid accidental lid displacement. All have sturdy, flat bases that resist bowing and warping for even growth and distortion-free microscopy. Vented lids improve gas exchange and are non-treated to minimize condensation. the stacking rings are also vented for better thermal equalization.
Cell Counting Chambers

eliminate the concerns of cleaning, drying, and handling fragile glass counting slides. the Cytoone cell counting chamber is designed for one-time use to guarantee cleanliness and save time. Counting chambers have integrated cover slips for error-free filling.
Large dishes have grip tabs for secure handling and less strain on smaller hands.
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From basic research to high-throughput screening, Molecular Devices expanding multi-mode microplate readers has you covered.
Unmatched assay Performance is now Within the Reach of any life Science laboratory with the FilterMax F3 & F5 Multi-Mode Readers
the patented Filtermax F series readers deliver a unique combination of value, performance, and sensitivity, putting high-performance multi-detection capabilities into the hands of any life science researcher. this unique filter-based platform delivers unmatched assay performance across a broad range of life science applications, and currently offers two models: the F3 with absorbance, fluorescence, and luminescence detection modes and the F5 with those same modes plus fluorescence polarization and time resolved fluorescence modes.
PRODUCTS in aCTiOn
enabLing TeChnOLOgy FOR high-ThROUghPUT aSSayS

Flexibility (available detection modes), performance (sensitivity, throughput), and cost are prime considerations in microplate reader selection, although the order of preference may differ for afford flexibility, wavelength scanning, and lower operating costs; hybrid systems constitute the best of both worlds. According to Xavier Amouretti, product manager at BioTek (Wingrowing market for ELISA assays. Marina Pekelis, senior product manager, describes absorbance, which is suitable for both singlepoint and kinetic assays, as the workhorse detection mode for Microplate Readers. Absorbance readers are popular due to their ease of use and intuitiveness, and are by far the most economical way to perform ELISA assays, says Pekelis. Users dont want to spend a lot of time figuring out an instrument when all they want to do is protein detection. Price is another positive: simple desktop absorbance MPRs cost less than $10,000, however, they can detect only one wavelength per well. To provide multi-parameter assays, Bio-Rad licensed the Luminex xMAP bead-based assay technology and turned it into a proprietary product, Bio-Plex. Experiments are run in 96-well plates, and detection occurs through flow cytometry. Like MPRs with multiple detection modes, absorbance readers can analyze one well at a time, which is slow but more reliable, or use a detector array that reads an entire row on the plate at once. In this mode reader, system software will automatically calculate an average well reading and generate statistics.
1311 Orleans Drive, Sunnyvale, CA 94089-1136 USA Phone: 1 408 747 1700 | Fax: 1 408 747 3601 Toll Free: 1 800 635 5577 www.moleculardevices.com
Absorbance readers are the most economical way to perform ELISA assays.
each market. Detection modes define the instruments experimental capabilities, while the optics determine spectral selectivity. Detection modes include top- and bottom-read fluorescence, fluorescence polarization, time-resolved fluorescence (TRF), time-resolved fluorescence energy transfer (TRFRET), AlphaScreen, absorbance, and luminescence. Absorbance and fluorescence intensity are the most widely used detection techniques, constituting more than half of all applications. Filters are selective for excitation and emission light, so two are required. Thirty percent of readers use monochromators, which tune in excitation and emission wavelengths through diffraction gratings controlled by the instrument software. Approximately 10 percent of readers are hybrid systems incorporating both monochromators and filters. Filters provide the highest sensitivity and read speed, while monochromators ooski, VT), users value flexibility, add-on functionality, and upgrade capability. Other desirable features include a full complement of detection modes, sample throughput, advanced optics, additional photomultiplier tubes for reading two wavelengths simultaneously (useful in FRET, TR-FRET, and fluorescence polarization), chargecoupled device cameras for imaging portions of plates or whole plates, and application-specific light sources such as pulsed lasers. Many researchers are investigating multiplexed assays, where more than one assay is performed in the same well, Amouretti notes. Multiplexing saves time, provides better data quality, and ensures that all assays are conducted under identical conditions. But this requires the reader to have good performance across a number of detection modes. Bio-Rad Laboratories (Hercules, CA), offers only absorbance-based readers to serve the large and
Read What you Want When You Want with the SpectraMax Paradigm Multi-Mode Microplate Reader
the patent-pending Spectramax Paradigm Platform is the only multi-mode detection platform on the market today that allows users to upgrade the functionality of their instrument directlyno service call, no factory shipment. upgrading is as easy as selecting from over 20 mix-and-match detection cartridges and inserting them into one or more of 12 instrument slots. A single cartridge can be changed in less than 2 minutesthe instrument can be fully reconfigured in 10 to 20 minutes. this unmatched flexibility provides a platform that can easily be adapted to meet constantly evolving application needs and ever advancing detection technologies while accommodating most budgets. with multiple detection modes, including AlphaScreen and HtrF, as well as 1536 and 3456-well microplate compatibility to support high-through screening, the Spectramax Paradigm reader is also ideal for assay development, lead identification, and lead optimization.
http://www.moldev.com/Products/Instruments/Microplate-Readers/Multi-Mode-Readers.html
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PCR ReagenTS LiFe SCienCe
ReCenTLy ReLeaSeD MicroPlaTe readerS SpectraMax Paradigm

Easily upgrade instrument functionality in < 2 minutes Excellent sensitivity and performance Up to 1,536- and 3,456-well plate format High-speed detection
Synergy H1 Hybrid
xeROxing geneS
www.biotek.com
Monochromator-based reader for UV-Vis absorbance, top/ bottom fluorescence and luminescence assays can be upgraded to a hybrid reader with the addition of a highperformance filter module Independent filter module with dichroic mirrors supports FP, TRF, TR-FRET and BRET assays BioTek
Molecular Devices www.moldev.com
Within a decade of its discovery in 1983, polymerase chain reaction (PCR) evolved into one of biologys most useful tools. PCR amplifies specific segments of genes so accurately that an early National Institutes of Health
These steps repeat 30 or 40 times in a thermocyling instrument. Each step doubles the quantity of DNA produced. Each of the steps involves specific reagents that are general for all PCR experiments: TRIS or
Anthos 2010
Includes four filters that cover wavelengths for most common absorbance assays A choice of software packages enables the user to connect to a PC and run both endpoint and kinetic assays with ease Single or dual wavelength measurements plus kinetic measurements maximize analysis options
Chameleon V
Both radiometric and non-radiometric assays can be carried out with ease Seven measurement technologies are available, such as fluorescence intensity Capable of reading Microtiter plates from 6 to 384 wells Includes MikroWin Lite 2000 instrument control software LabLogic Systems www.lablogic.com
PCR reagents are sold individually or as master mixes.

publication dubbed the technique Xeroxing DNA. Today, PCR is used routinely in dozens of approved medical tests in diagnostic labs and physician offices, and by millions of scientists worldwide. Understanding PCR reagents requires a bit of knowledge about the reaction itself. The steps involved in PCR are: Denaturation: Heating the sample to separate the target DNAs double helix into individual strands. Denaturation takes place in specialized buffers. Annealing: Cooling the sample in the presence of an antisense (or complementary) DNA sequence, known as a primer (there are two primers, one for each strand separated in the first step). Elongation: Addition of an enzyme, polymerase, that builds the new DNA strands, and deoxynucleotide triphosphates (dNTPs), which are building blocks for the new DNA copies. some other buffer, enzyme, magnesium chloride (necessary for enzyme activity) and the dNTPs. Primers and templates are unique for every amplification. Since the patents on Taq polymerase expired, vendors have been free to develop and sell their own versions, resulting in downward price pressures. PCR reagents are sold individually or as master mixes. The separate purchase route allows end users to fine-tune PCR reactions with favored (or less expensive) reagents, but this requires a fair degree of expertise. The debut of real-time or quantitative PCR (qPCR) allows investigators to quantify the number of copies made and, by backtracking, the concentration of the template in the sample. Purchase decisions for PCR reagents are based on performance (speed of analysis, fidelity of DNA-copying) and price. Instruments used to play a role in choice of reagents, says Jeff
Biochrom www.biochrom.co.uk
MicroPlaTe reader ManufacTurerS

Applied Biosystems Beckman Coulter Berthold Technologies Biochrom Bio-Rad BioTek Instrutments BMG Labtech Douglas Scientific Molecular Devices PerkinElmer Tecan Thermo Fisher Scientific Turner Biosystems www.appliedbiosystems.com www.beckman.com www.berthold.com www.biochrom-us.com www.bio-rad.com www.biotek.com www.bmglabtech.com www.douglasscientific.com www.moleculardevices.com www.perkinelmer.com www.tecan-us.com www.thermoscientific.com www.turnerbiosystems.com
Get a better reaction
Williams, Ph.D., president of Lucigen (Middleton, WI), but these days temperature cycling instruments have fairly similar performance characteristics and should not be a factor in reagent selection. Recently, environmental scientists detected the presence of Asian carp in the Great Lakes not by capturing a fish, but by amplifying their genes with PCR. Similarly, beer brewers use PCR to uncover bacterial contamination in their process. PCR may also be used at relatively large scale to manufacture genes for gene therapy or vaccine work. One instrument-related issue that still concerns analysts is temperature. Specialized experiments may require reaching precise temperatures for exact times. The wrong temperature can cause amplification of the wrong DNA segment, notes Ms. Mason of Agilent, who believes that instrumentation is lagging behind reagents in terms of speed and quantity of DNA produced, particularly with multiplexing (several samples in one run) becoming more common. Screening experiments dont have to be as precise as quantitation. It depends on what youre trying to achieve with a particular amplification.
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PCR ReagenTS
SPEX CertiPrep
PRODUCTS in aCTiOn
ReCenTLy ReLeaSeD Pcr reaGenTS qPCR Kits for SYBR Green and Probe Chemistries
DyNAmo ColorFlash
2x Master Mix contains a blue dye, and a separate sample buffer contains a yellow dye qPCR reaction mix containing both components is green The dyes do not affect the specificity or sensitivity of qPCR assays Finnzymes www.finnzymes.com
qPCR Reagents
Brilliant III
Novel fast Taq mutant for qPCR results in under 35 minutes Deliver qPCR results in about 40 minutes Optimized fast cycling formulation ensures reliable and reproducible data with shorter run times Convenient pre-blended formulations compatible with any sequence-specific probe detection chemistry Agilent Technologies www.agilent.com
SPex CertiPrep has introduced a new line of Pure and ultra-Pure Fusion Fluxes and Additives. Both lines are of a high purity, with the ultra-Pure line being the purest on the market at 99.998% pure. these fluxes are made from a micro Bead formula that ensures the same ratio of components is in each bead. this new formula has no harmful dust to clog your instruments which also reduces weighing times. our highly standardized manufacturing process produces identical batches with no appreciable lot to lot variations, thus maintaining a high level of consistency and quality.
203 Norcross Ave, Metuchen, NJ 08840 Phone: 1-800-LAB-SPEX Fax: 732-603-9647 E-mail: crmsales@spexcsp.com www.spexcsp.com
Fusion is an extremely effective method to prepare oxides, sulfides, fluorides, ferroalloys, and other metals for analysis by xrF, AA, iCP, dCP, etc. the samples are (if necessary) pulverized and mixed with a flux; this mixture is heated until the flux melts and the sample dissolves in it, yielding a clear, homogeneous melt. the melt can be cast as a glass disc for xrF or dissolved in dilute acids for analysis in solution form. in many cases fusion fluxing is simpler and the analytical results more accurate than if the sample was prepared by conventional acid dissolution or pressed powder methods. SPex CeriPreps new line of Pure and ultraPure Fusion Fluxes are available in a wide variety of compositions for the most popular fusion methods. Fluxes containing additives are pre-fused for better accuracy. our micro Bead formula ensures consistent component ratios while eliminating harmful dust and decreasing weighing time.
every SPex CertiPrep Fusion Flux product comes with a Certificate of Analysis stating impurity values. Custom Fusion Flux mixes are available upon request. Also, take advantage of quantity discounts for orders of 10 or more kilograms. SPex CertiPrep Fusion Flux is made by the leading manufacturer of Certified reference materials. SPex CertiPrep has been in business for over half a century. Contact us today to request a free sample of the SPex CertiPrep Fusion Flux of your choice. no purchase necessary. For a limited time only, also receive a free SPexFusionFlux Scoop!
qPCR Master Mixes

USB VeriQuest
Includes dUTP and uracil DNA glycosylase (UDG) Features ROX compatibility with all ABI and Stratagene instruments Includes chemically modified VeriQuest Taq DNA Polymerase in a proprietary reaction buffer
qPCR Reagents
SsoFast Probes Supermix

Enables researchers using fluorogenic probes to enhance the speed, reliability and sensitivity of their qPCR experiments Yields fast duplex qPCR results in 30 minutes or less Fully compatible with universal cycling conditions and a wide range of primer/probe concentrations without requiring additional optimization Bio-Rad www.bio-rad.com
Affymetrix/USB www.affymetrix.com
Pcr reaGenT ManufacTurerS

Affymetrix/USB www.affymetrix.com Invitrogen Labnet International Lucigen Promega Sigma-Aldrich SPEX CertiPrep SABiosciences A QIAGEN Company www.invitrogen.com www.labnetlink.com www.lucigen.com www.promega.com www.sial.com www.spexcsp.com www.sabiosciences.com
Agilent Technologies Stratagene Products www.stratagene.com Ambion AnaSpec Applied Biosystems Bio-Rad Bulldog Bio EMD Chemicals Finnzymes GFS Chemicals www.ambion.com www.anaspec.com www.appliedbiosystems.com www.bio-rad.com www.bulldog-bio.com www.emdchemicals.com www.finnzymes.us www.gfschemicals.com
New England Biolabs www.neb.com Roche Applied Science www.roche-applied-science.com
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Rnai ReagenTS
ReCenTLy ReLeaSeD rnai reaGenTS
BLOCK-iT
RNA interference (RNAi) uses short strands of synthetic ribonucleic acid (RNA) to silence or knock down genes implicated in certain phenotypesmost commonly (but not limited to) diseases. The interference occurs when interfering strands bind to complementary, naturally occurring RNA according to standard base-pairing rules. Unlike antisense technology, which operates on DNA, RNAi works by silencing RNA, which is the immediate precursor of proteins implicated in the phenotype of interest. Reagents consist principally of the interfering RNA construct and a transfection agent for introducing the RNA into cells. The most commonly used interfering RNAs are the short (19 to about 25 nucleotides) interfering RNAs (siRNAs) and short hairpin
enabLeRS OF The bRave neW WORLD OF Rna inTeRFeRenCe

shRNA reagents are introduced in plasmid format, which means the target cells can incorporate the silencing agent into their genome and pass it on to offspring. shRNA is absolutely needed when the phenotype takes longer than about two weeks to develop, says Steven Suchyta of Sigma-Aldrich (St. Louis, MO). shRNA may also be introduced into cells via a virus transfection agent. Some vendors offer lentivirus transfection reagents that provide long-term, stable knockdown in almost any mammalian cell. Vendors also offer peptide-based reagents. Reagent companies sell RNAi reagents as virtual kits consisting of the shRNA or siRNA sequences and an appropriate transfection reagent. Vendors usually guarantee that a certain percentage of multiple knockdown constructs they sell for a particular target RNA will succeed. Users can monitor the progress of their knockdown by performing a before-and-after Western blot to determine if the protein coded by the putative knockdown gene is still being produced. Length is a critical attribute of siRNA and shRNA reagents. In nature, interfering RNA species
Complete Dicer RNAi Kit

Provides an easy alternative to using synthetic short RNA oligonucleotides for RNAi experiments Includes an siRNA purification module specifically developed to isolate pure siRNA from the Dicer reaction Provides an effective way to screen as many as five genes in a mammalian model Invitrogen www.invitrogen.com
Transductin
Peptide Delivery System for dsRNA
Approximately 75 percent of the RNAi reagent market uses siRNA.

RNA (shRNA). Approximately 75 percent of the RNAi reagent market uses siRNA, according to Chris Cunning, Ph.D., senior manager of market development at Invitrogen (Carlsbad, CA). Both reagent types bind to complimentary sequences on genes.
are usually between 20 and 25 nucleotides in length. Longer constructs could theoretically work better since they cover a greater fraction of the target gene, but 20 to 25 nucleotide lengths are ideal for entry into RISC complexes. Furthermore, larger genes tend to be recognized by cells as viruses, which induces an undesirable interferon response. RNAi may eventually have greater impact on biology than polymerase chain reaction (PCR), although RNAi can be considered more complex than PCR. The main obstacle is introducing the interfering RNA sequence into the cell, into the location of the target gene, and then getting it to bind to and inactivate the target. Success with one sequence, transfection agent, and cell does not guarantee success when one variable changes. Delivering interfering RNA into whole organisms (vs. cells) presents even greater challenges, but the potential rewards are also high. Whole-organism or whole-tissue knockdowns would provide new opportunities in drug testing and, eventually, for human therapy.
Uses the cells own mechanism for uptake to avoid toxicity and immune response often observed with other reagents Works through a mechanism distinct from cationic lipids Can be used to deliver dsRNAs to most cell lines, including primary cells and ES cells Integrated DNA Technologies www.idtdna.com
siLentFect
Lipid Reagent for RNAi

Consists of a proprietary cationic compound and a co-lipid Low volumes of lipid required per transfection help to minimize cell stress Offers excellent performance at culture densities between 50 and 90% and in the presence or absence of serum-containing medium
Turbo Dicer
siRNA Generation Kit
Bio-Rad www.bio-rad.com
Enables easy generation of a large number of siRNAs from full-length target genes Allows the user to quickly produce multiple siRNA species against target mRNA Contains everything required for preparing double stranded RNA from target gene(s), dsRNA cleavage, siRNA cleanup and transfection AMSBIO www.amsbio.com
rnai reaGenT ManufacTurerS

Ambion AMSBIO Bio-Rad Bio-Synthesis Integrated DNA Technologies Invitrogen Luminex Corporation Promega Sigma-Aldrich SPEX CertiPrep Taconic Thermo Fisher Scientific, Dharmacon www.ambion.com www.amsbio.com www.bio-rad.com www.biosyn.com www.idtdna.com www.invitrogen.com www.luminexcorp.com www.promega.com www.sigmaaldrich.com www.spexcsp.com www.taconic.com www.dharmacon.com
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anaLyTiCaL baLanCeS
STRaighTFORWaRD, COMPLex PURChaSing DeCiSiOnS

Analytical balances use enclosed weigh pans and come in two basic varieties: microbalances (accurate to 1 g) and semi-microbalances (10 g). Modern balances come equipped with built-in applications for piece counting, density calculations, statistical analyses and other straightforward calculations. Additional features include color and/ or touch screens, faster microprocessors and stability times, better repeatability, hands-free operation, multiple interface options for open-architecture connectivity, and regulatory compliance (e.g., for pharmaceuticals). Like many other instrument types, balances have come to rely heavily on electronics. Compensating physical weights were replaced long ago by strain gauges and frequencymodulated force measurement in low-end balances, and by electromagnetic force compensation in higher-end analytical instruments. According to Ryan Titmas, VP at Sartorius Mechatronics America (Bohemia, NY), the price of a balance is a function of its capacity (maximum weight handled) to readability (resolution) ratio, plus number of features. Users can expect to pay anywhere from $2,000 to $8,000 for an analytical balance. grams fall short, says Steve Wildberger, product coordinator at Shimadzu Scientific Instruments (Columbia, MD). Users always want an application to do one thing more than it can. The problem, Wildberger says, results from balances limited memory and data processing capabilities. This berger, the decision tree reduces to instrument capacity and resolution (the first criteria), followed by calibration capabilities (internal or external) and interface/data features. And in the experience of Ryan Titmas of Sartorius, Most users take the middle road and choose a solid
the price of a balance is a function of its capacity (maximum weight handled) to readability (resolution) ratio, plus number of features.
has, in turn, led to the emergence of wedge software packages that interface the instrument to the labs information backbone to provide automated data entry and other functions. These packages, Wildberger argues, add an additional level of complexity for users, and a higher validation burden for labs operating in regulated industries. The future, he says, belongs to [instruments] that act as sensors or input devices, directing data into familiar computing environments and applications, for example Microsoft Windows spreadsheets. Microsoft Excel has tremendous capability for handling weighing operations, including statistical analysis and checkweighing [for determining that a piece falls within a specified mass range]. analytical balance with accuracy, speed, and a few other features. But Ian Ciesniewski, technical director at Mettler Toledo (Columbus, OH), recommends that users first generate a design qualification that accounts for the acceptable weighing uncertainty, and from this figure define the required precision (repeatability). Repeatability is adversely affected by changes in the laboratory environment, which can cause both acute and long-term problems, he says. We recommend selecting a balance that is better than required by a safety factor of two or three. This will minimize out-of-tolerances and avoid the dreaded do not use notice. Other factors to consider, Ciesniewski says, are ergonomic and productivity features such as the ability to enter data on sample identity, batch or users; touch-screen operation; color screens; built-in applications; communication and software capabilities; and maintenance/ calibration requirements.
Thank you Rice Lake!

Maybe you could rate your customer service levels at a 10 rather than a 5. I am pleased to say that I have never worked with an organization that is so helpful and knowledgeable about product, pricing, and overall service. A four-day turn-over rate is phenomenal and your team of customer service and calibration techs are great to work with. Mary Anderson, DiaSorin, Inc
Customers with unique weighing needs often find that preloaded pro-
application limits
Purchase factors
Some experts believe the choice of analytical balance is relatively straightforward. For Steve Wild-
USA 800-472-6703 www.ricelake.com/weights

2010 Rice Lake Weighing Systems
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anaLyTiCaL baLanCeS
ReCenTLy ReLeaSeD analYTical BalanceS PW TA Series
Measures to 0.1 mg Bi-directional RS-232 interface enables data transfer from the balance to computers or printers Features solid, durable metal construction and internal motorized calibration Built-in applications include density, percentage and comparison weighing Adam Equipment www.adamequipment.com
Features mono-metal Tuning-fork Sensor Technology Includes internal calibration and RS-232C interface Includes an anti-electrostatic 360 transparent windshield Large back-lit 16.5 mm LCD screen displays 8 digits Rice Lake Weighing Systems www.ricelake.com
XA/Y Series
Includes a spacious weighing chamber with an automatic opening function Indicator can detach from weighing chamber, to reduce the risk of shocks and vibrations Features internal calibration, triggered by time flow or temperature conditions Includes a 5.7-inch touch panel color display RADWAG www.radwag.com
Complete product bundles consist of an Excellence XP/XS balance, corresponding accessories and LabX 2010 software One Click performs all calculations and documentation automatically, shortening the time to prepare a standard solution from 15 minutes to less than four The complete solution can be customized to meet individual process requirements
One Click Weighing Solution
nd amazing
buys!
Register today to run ads, bid in LabAuctions, or contact buyers and sellers. LabX showcases over 190,000 listings of new, surplus, preowned lab equipment, and supplies. Visit LabX today and get the product youve always wanted NOW!
Mettler Toledo www.mt.com
analYTical Balance ManufacTurerS

A and D Weighing Adam Equipment Denver Instrument Fulcrum Gram Precision Intelligent Weighing Technology Mettler Toledo Ohaus Corporation RADWAG Rice Lake Weighing Sartorius Scientech Shimadzu Scientific Instruments Thermo Fisher Scientific UDY Corporation www.andweighing.com www.adamequipment.com www.denverinstrumentusa.com www.fulcruminc.net www.gramprecision.com www.intelligentwt.com www.mt.com www.ohaus.com www.radwagusa.com www.ricelake.com www.sartorius-mechatronics.com www.scientech-inc.com www.ssi.shimadzu.com www.fishersci.com www.udyone.com
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BUY & SELL EQUIPMENT ONLINE AUCTIONS NEW PRODUCT INTRODUCTIONS GREAT DEALS
baSiC Lab
biOLOgiCaL SaFeTy CabineTS
PRODUCTS in aCTiOn
BSCs are distinct from other safety enclosures. Fume hoods pull air over the work item and into the environment through a vent, whereas controlled atmosphere glove boxes are completely enclosed, protecting both users and samples through an airtight barrier. A distinguishing component of BSCs is their use of high-efficiency particle air (HEPA) filters, which scrub effluent between 99.5 percent and 99.99 percent of airborne particles, or at least 99.97 percent of particles larger than 3 microns.
nOT yOUR DaDDyS FUMe hOOD

are reserved for highly contagious or virulent biological samples. Class II A2 cabinets are by far the most common BSCs in use today, comprising about 95 percent of installations, according to David Phillips, technical applications specialist at Thermo Scientific (Asheville, NC). An ongoing controversy for specifying certain Class II cabinet types involves NSF Standard 49, which states that Class II A2 and B2 cabinets are designed to handle minute amounts of toxic chemicals and radionuclides. But nobody has
no third-party organizations provide energy-efficiency ratings for cabinets.

Class I BSCs protect personnel and the environment only. Samples are vulnerable because workspace air is swept over them before filtration and venting. Class II cabinets represent a broad category, with varying capabilities that are further subdivided into categories A1, A2, B1 and B2. The main differentiator between Class I and Class II BSCs is that Class II cabinets employ a HEPA-filtered, vertical, unidirectional airflow within the work area. Class III BSCs, which provide the highest level of protection to workers and samples, defined the term minute quantitatively, admits Phillips, who works on the NSF joint committee that determines BSC specifications. To satisfy whatever that requirement might be, and to err on the side of caution, most laboratories automatically specify the use of Class II B2 cabinets, which Phillips describes as complex and 10 times trickier to run than Class II A2 cabinets. A lot of people get stuck with B2s, but half of them should never have been installed. Users would be much better served by canopied A2 cabinets.
Jim Hunter, senior project engineer at Labconco (Kansas City, MO.), suggests using B-type cabinets in situations where workers are consistently working with volatile toxic agents, isotopes or anticancer drugs that they dont want coming back into the lab. Otherwise, the associated costs are simply not worth it. B cabinets cost a lot of money and use a lot of energy, says Hunter. Unfortunately, architects assume a B cabinet is always better because its more expensive or because the letter B comes after A in the alphabet. All too often they simply override a customers decision on which type of cabinet to purchase. An improvement in operating costs and eco-friendliness is energy consumption. Oldermodel BSCs were energy hogs, but todays units are downright miserly. However, unlike other laboratory products, purchasers of BSCs must perform their own due diligence, as no third-party organizations provide energy-efficiency ratings for cabinets.
The Baker Companys FlexaIR Canopy Exhaust Connection Saves Energy and Provides Safer BSC Performance
the Baker Companys FlexAir canopy exhaust connection offers significant energy and cost savings to the laboratory, without sacrificing safety and performance. the Baker FlexAir technology combines the security of a traditional canopy (or thimble) exhaust connection with the lower exhaust flows of a traditional hard exhaust connection. Bakers FlexAir works by utilizing dynamic front and side panels that open or close depending upon the variances in exhaust flow rate. with a FlexAir canopy exhaust connection (CeC), only the minimum amount of air necessary to achieve cabinet exhaust containment is used, Adding a FlexAir CeC reduces the amount of exhaust required to operate a biological safety cabinet by up to 18%. Additionally, safety for both the product and personnel exceed nSF standards even when the buildings exhaust system fails. For more information, please visit: www.bakerco.com
P.O. Drawer E, Sanford, ME 04073 (207) 324-8773 (800) 992-2537 Fax: (207) 324-3869 www.bakerco.com
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Biological safety caBinets
Recently Released BioLogicaL Safety caBinetS Purifier Cell Logic Labculture
Scope-Ready feature allows a customer-supplied microscope to be integrated into the cabinet Pure-Vu Seal allows a clear view into the cabinet Features a Stand-Still Isolation Platform which reduces vibration up to 300% Temp-Zone option keeps heated or chilled samples at their ideal temperatures Labconco www.labconco.com
Uses dual ULPA filters with 99.999% efficiency for particle sizes between 0.1 to 0.3 microns Features a raised airflow grille to maintain safety by preventing blockage Features a frameless sash for easy cleaning ISOCIDE antimicrobial coating on all surfaces minimizes contamination ESCO www.escoglobal.com
1300 Series A2 BSC
Uses a smaller aperture to extend the life of the HEPA filters by 20 percent SmartFlow airflow compensation system maintains a safe environment for the user Low airflow requirements can reduce noise levels to as low as 62 dB(A) Requires only 180 watts to operate
Features DC/ECM motor to consume less energy, minimize vibration and lower noise output Includes HEPEX Zero Leak Airflow System to ensure uniform airflow and proper filter loading Made of 16 gauge type 304 stainless steel as a single piece shell, eliminating leaks Choice of three control panel options, all with TouchLink LCD touch screen controls NuAire www.nuaire.com
LabGard ES (Energy Saver)
Thermo Fisher Scientific www.thermoscientific.com
BioLogicaL Safety caBinet ManUfactUReRS

Air Science USA AirClean Systems Captair Erlab ESCO Flow Sciences Germfree HEMCO www.air-science.com www.aircleansystems.com www.captair.com www.erlab.com www.escocorp.com www.flowsciences.com www.germfree.com www.hemcocorp.com Labconco Microzone Misonix NuAire Sentry Air Systems Terra Universal The Baker Company www.labconco.com www.microzone.com www.misonix.com www.nuaire.com www.sentryair.com www.terrauniversal.com www.bakerco.com
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Biological sHaKeRs & stiRReRs
pRoducts in action
essential tools at MacRo scale; autoMation fRiendly foR MiniatuRized assays

Biological shakers are used to agitate a collection of biological samples simultaneously. Shakers consist of a motor attached to a flat surface, with fasteners for securing labware whose contents require mixing. All points on the surface move in the same fashion, either back and forth (reciprocal shakers) or in a circular motion (orbital shakers). Shaker flasks are similar in design to Erlenmeyer flasks but may have a baffled bottom to promote mixing. The principal application of shakers is for growing yeast, bacteria, or mammalian cells in specialized containers known as shaker bottles. Shaking promotes the growth of cells and microorganisms by improving aeration and oxygen transfer and by promoting more efficient mixing of cells with food and nutrients. Biological shakers generally operate at temperatures between ambient and 37C, but some models offer refrigeration, and hightemperature instruments operate at up to 100C. Orbital shakers can accept vessels of almost any size or shape, from Erlenmeyer flasks to test tubes and vials, as well as trays for staining and destaining electrophoresis gels, notes Janet OBryan, product manager at Thermo Fisher Scientific (Vernon Hills, Ill.) Choosing a shaker comes down to such features as heating/cooling capability, capacity, shaking speed, orbital vs. reciprocating motion, ease of use, programmability and footprint. With research budgets tight and lab space even tighter, groups or departments are increasingly sharing shakers. Customers frequently choose models based on how much space they require. Its highly desirable to be able to stack shakers, pizza oven style, and keep them in a shared equipment room, Like their large-scale counterparts, microplate shakers operate at variable, user-specified speeds and employ mechanical agitationrocking or circular (orbital) movementto mix components within microplate wells. A typical microplate shaker handles all common plate densities, rotates at up to 1,500 rpm, and accommodates sample volumes of up to 250 microliters. Engineering issues come into play for mixing very small samples. As sample volume decreases, mixing efficiently becomes an engineering problem, as the fluids low mass causes it to adhere to surfaces, says Sriram Kumaraswamy, Ph.D., product manager at ForteBio (Menlo Park, Calif.). Plate shakers become the slow step in high-throughput workflows unless they are interoperable within a larger microtiter plate-handling environment, which generally includes a microplate handler. Essential integration features include a spring lock to retain the plate against the shaking surface and a robotfriendly lock/unlock mechanism. Plate shakers, like other components in a microplate-handling system, should be automation-friendly, notes Dr. Kumaraswamy.
Boekel Scientific Heat/ Cool Thermal Mixer

The H-C Mixer offers both mixing and temperature control that can be applied to a wide range of laboratory applications. The H-C Mixer has advanced temperature control from 18C below ambient temperature to 99C and accommodates a variety of Micro tube and Plate configurations. In addition to the controlled temperature mixing function, the H-C Mixer also offers the additional functionality of programmable mix-pause operation and programmable timed temperature modes. The user-friendly control interface and 10 programmable settings per operation mode and will contribute to an increase in productivity of daily sample preparation and laboratory procedures. The H-C mixer is the ideal instrument when preparing samples for various life science applications. From denaturing DNA, RNA or Proteins, to Plasmid purification and Enzyme reactions, the precision temperature control and mixing function will provide an efficient and reproducible solution for many sample preparation protocols. The H-C Mixer offers three programmable operation modes with up to ten programmable settings per mode. The first mode is used to control the temperature and mixing control for a set or unlimited period of time. With the shaking feature off, users can control the temperature from 18C below ambient temperature to 99C while preparing samples. With the addition of the programmable mixing control users can control the mixing function from 250-1400 rpms allowing for a very gentle or vigorous mixing function. The H-C Mixer is well suited for many controlled temperature mixing applications in the life science laboratory. The second mode allows the researcher to control the temperature of a sample with a mix/pause function that can be programmed to control the length of time that the samples are mixed and paused for a controlled period of time. The third mode allows the researcher to program a set temperature and mixing speed for a controlled period of time and then change to a second set temperature and mixing speed for a second controlled period of time. This functional programmability offers researchers the ability to program the mixer for controlled and reproducible enzyme reactions or denaturation protocols. With the ability to program up to ten user settings per mode, the H-C Mixer allows multiple user functionality while minimizing the time requirement to reprogram end user protocols.
855 Pennsylvania Blvd. Feasterville, PA 19053 Phone 800-336-6929 www.boekelsci.com
Customers frequently choose models based on how much space they require.
OBryan said. Customers also value ease of usethe ability to utilize shakers fully, out of the box, with labware of any shape and size. Labware flexibility is particularly critical for shared instruments.
Microplate shakers facilitate chemical and mechanical cell lysis and the homogenization of inert samples, cells, or cell components; they also help emulsify liquid-liquid and solid-liquid mixtures. An efficient shaker can also reduce the time by half for assays that depend on rapid agitation.
Microplate shakers
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Biological sHaKeRs & stiRReRs
Recently Released Biological sHaKeRs & stiRReRs Biological Shakers

Innova Series
Feature programmable changes to multiple parameters on a timed basis Temperature is regulated 0.1C from 30C to 40C in incubated models Features acceleration-deceleration circuit to prevent sudden starts and stops Temperature-controlled models include thermostat fail-safe, shutting off heaters if high limit is exceeded New Brunswick Scientific www.nbsc.com
Platform Shaker
PSU-20
Can be programmed to provide orbital, reciprocation and vibration mixing functions Three functions can be set for continuous or timed operation in a single function mode or any combination of all three A variety of interchangeable platforms accommodate different sized flasks, beakers, Petri dishes and other vessels Grant Instruments www.grantsci.com
Digital Magnetic Stirrers

170/220 mm
Two sizes: 6.25 plate with a 4-liter load capacity; 8.25 plate with a 5-liter load capacity Supplied with two non-slip silicon plate pads Wide speed range of 30 to 2,000 rpms Large, bright VFD readout allows easy monitoring of stirring speed Jeio Tech www.jeiotech.com/eng
Zero Torque Overhead Stirrer

BDC Series
Redesign of the BDC1850, BDC3030 and BDC6015 hightorque stirrers includes the addition of a zero torque button Allows the user to zero the torque at any stage of mixing, or zero out any additional overhead torque created by the mixing paddle and chuck before mixing starts Caframo www.caframo.com
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BioLogicaL SHaKeR & StiRReR ManUfactUReRS

Bel-Art Products BioCold Boekel Scientific Caframo Eberbach Grant Instruments Heidolph IKA Works Jeio Tech Kinematica Labnet International www.belart.com www.biocold.com www.boekelsci.com www.caframo.com www.eberbachlabtools.com www.grantsci.com www.heidolph.com www.ika.net www.jeiotech.com www.kinematica-inc.com www.labnetlink.com Labnics Equipment Pro Scientific Sartorius Stedim Scientific Industries Silverson Machines Stovall Life Science Thermo Scientific Torrey Pines Scientific Troemner UDY Corporation www.labnics.com www.proscientific.com www.sartorius-stedim.com www.scientificindustries.com www.silverson.com www.slscience.com www.thermo.com www.torreypinesscientific.com www.troemner.com www.udyone.com
New Brunswick Scientific www.nbsc.com
Yamato Scientific America www.yamato-usa.com
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EPPENDORF - MICROCENTRIFUGATION REDEFINED
The Eppendorf approach to product development isand has always beenabout giving you more. More quality. More innovation. As a result, the new line of Eppendorf microcentrifuges delivers product performance that goes far beyond speed and capacity to benefit you and your work environment. In addition to the speed, capacity and versatility you need for all your applications, our new Eppendorf microcentrifuges offer unparalleled ergonomic operation (e.g., whisper quiet operation, soft-touch lid closures, intuitive control) , superior temperature management for maximum sample protection and the Eppendorf quality youve come to expect. Some say that its the little things in life that make a difference. We couldnt agree more. Reward yourself with an Eppendorf centrifuge. The new Eppendorf line of microcentrifuges consists of Models 5418 / 5418 R (18-place capacity for 1.5/2.0ml tubes, speed up to 16,873 x g) for medium throughput needs The new lab standard Centrifuges 5424 / 5424 R (24-place capacity, speed up to 21,130 x g) Unique cross-over Centrifuges 5430 / 5430 R (30-place capacity, speed up to 30,130 x g). These 2 models combine the best features of a microcentrifuge (small footprint) and multipurpose centrifuge (versatility) in one instrument. These centrifuges spin rotors for Eppendorf tubes and PCR strips as you would expect from any microcentrifuge. But thats not all. In a compact sizejust over a foot of bench spaceModels 5430 and 5430 R also accommodate a fixed-angle rotor for 15/50 ml conical tubes, Vacutainer, 10 to 50 ml OakRidge tubes, cryo and HPLC vials as well as a swing-bucket rotor for MTP and PCR plates. Until now, this has only been possible with large multipurpose centrifuges. As a commitment to the environment and future generations we constantly look for ways to improve the eco-friendliness of our products. This already started many years ago when we switched all our refrigerated models to CFC-free refrigerants with zero ozone depletion potential. With our new line of microcentrifuges, we have now reached a level of performance and energy efficiency that defines an entirely new laboratory standard. By equipping our new refrigerated microcentrifuges 5418 R, 5424 R, and 5430 R with the latest innovations in cooling technology, were able to significantly reduce the overall energy consumption: Up to 20% improved energy-efficiency compared to predecessor models with optimized motor design and electronic parts (5415 C vs. 5418, 20 min run at 16,000 x g) Optimized insulation material of rotor chamber improves temperature efficiency. 60% less energy consumption with Model 5424 R during precooling to 4C due to a unique, patent-pending compressor technology that allows for fast, 8 min precooling of centrifuge and rotor. Up to 47% energy savings (overnight) are achieved due to unique ECO shut-off feature that deactivates the compressor after 8 hours of non-use. This feature comes standard with all refrigerated Eppendorf centrifuges. Up to 79% lower energy consumption with unique FastTemppro function (Model 5430 R). FastTemppro allows for automated pre-cooling based on pre-programmable time and date. Turn off the compressor and let FastTemppro take care of pre-cooling in the morning. There are a lot of centrifuges that offer speed and capacity. Eppendorf decided to take centrifugation to the next level and offer you features that benefit you, your applications and the environment.
pRoducts in action
continuuM of options and featuRes foR HigHuse devices

Centrifuges are among a select group of laboratory instruments that are as scalable as they are configurable. Individuals who have used benchtop centrifuges that handle sub-milliliter volumes may be surprised to learn that centrifuges some as large as roomsare used in industrial processing. Basic centrifuge designs are simple, consisting of an enclosed compartment inside which a rotor spins rapidly. Rotors, which can usually be interchanged, contain equally spaced openings into which sample tubes are inserted. Samples will either spin at a fixed angle relative to the rotating axis or swing out to perpendicular under centripetal force as the rotor speed increases. Forces generated as the rotor spins cause components in the sample to migrate toward the bottom of the sample tube, according to weight or density. Entry-level mini-centrifuges easily fit on a benchtop, operate at a single, relatively low speed, generate low gravitational (g) forces, and cost only a few hundred dollars. Minis are used for samples whose components are easily separated by density. Most medical and veterinary office centrifuges are of this type. The next level up, compact benchtop centrifuges, spin tubes of up to about 2 mL and create tens of thousands of gs. Researchers use them to separate DNA, proteins, and cellular components. There are many ways to differentiate centrifuges by type, speed, and features. Beckman Coulter (Fullerton, Calif.), for example, divides its product line into three basic platforms: benchtop devices operating at up to about 10,000 How fast does your sample need to spin to achieve the desired separation? Is an angled rotor or a swing-out rotor best for your application? Does your sample require refrigeration? What is the range of applications you are likely to encounter? Angled vs. swing-out tube design affects speed and g-force, and provides sample collection options (spin-out is slower but provides a clean pellet). Refrigeration is desirable because samples heat up during a long run. It all comes down to your expected application range and the likelihood that the instrument you buy will be flexible enough to meet your needs. Price was conspicuously absent from the list because lab centrifuges tend to be inexpensive compared with other high-use lab instruments. The price sweet spot of about $300 for unrefrigerated, single-speed mini-centrifuges up to about $6,000 for high-speed, refrigerated benchtop instruments covers most applications in the life sciences and other industries.
In the US Tel: 800-645-3050 In CANADA Tel: 800-263-8715 www.eppendorf.com
Silence
Speed
Simplicity
Lab centrifuges tend to be inexpensive compared with other high-use lab instruments.
rpm, washing machine centrifuges that provide up to about 100,000 g, and ultracentrifuges that deliver in excess of one million g. In fact, one could argue that all centrifuges exist along a continuum of features that may be mixed and matched, which include g-force generated, sample tube size, refrigeration capabilities, rotation angle, computerization, and others. Michael Rosenblum, marketing VP at LabNet International (Edison, NJ), offers the following considerations when purchasing a lab centrifuge: What size tubes do you expect to run?
Centrifuge 5430 R (refrigerated): Microcentrifuge with multipurpose capabilities spins tubes from 0.2 to 50ml as well as MTP and PCR plates. Fits on your lab bench - only 15 inches wide.
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Recently Released centRifUgeS 5430 R KITMAN-T24
Spins tubes from 0.2 ml to 50 ml as well as microplates Features a maximum speed of 17,500 rpm Small footprint requires only 15 inches of bench space A refrigerated model is also available
Includes a multi-memory function that allows recall of up to 6 sets of operating conditions Features a power-saving mode, which activates after a period of standby Set temperature of 4C from ambient temperature of 25C is reached in about 5 minutes When temperature inside chamber increases in power saving mode, the system will automatically cool the chamber TOMY www.digital-biology.co.jp
Provides fast separations for samples as small as 175 l up to 13.5 ml Features speeds up to 150,000 rpm and RCF of more than 1,000,000 x g Includes a customizable touch-screen user interface for up to 12 individuals Includes software that enables users to specfy up to five steps, set multiple programs or delay start time Beckman Coulter www.beckmancoulter.com
Optima MAX-XP
Features a maximum speed of 15,000 rpm Features infinitely variable refrigeration from -20C to 40C Maximum capacity in swing-out rotor: 4 x 290 ml Maximum capacity in an angle rotor: 6 x 94 ml iHettich www.hettichlab.com
ROTINA 380 R
2010 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
centRifuges
Do it with one glove tied behind your labcoat.
Seal buckets with a simple one-handed snap. Its just one way
centRifUge ManUfactUReRS
Ample Scientific Beckman Coulter Eppendorf North America Helmer Hettich Labnet International MidSci New Brunswick Scientific Sartorius Stedim Thermo Fisher Scientific Tomy Tech USA www.amplescientific.com www.beckman.com www.eppendorfna.com www.helmerinc.com www.hettweb.com www.labnetlink.com www.midsci.com www.nbsc.com www.sartorius-stedim.com www.thermo.com www.tomytech.com
Thermo Scientific centrifuges make your life easier because theyre designed for the way you work. With superior performance, reliability and rotor support built-in its the little things that are no small matter when it comes to keeping you happy and productive: Innovative and certified ClickSeal bucket sealing system: glove-friendly, one-handed snap-on covers replace screw caps and clips. Easy and secure AutoLock rotor management: exchange rotors in less than 3 seconds with the push of a button, switching from high capacity to high performance. Thermo Scientific Fiberlite carbon fiber rotors: speed, versatility and corrosion-resistant design to maximize performance. Getting the max out of your centrifuge shouldnt take extra work. To learn more about Thermo Scientific general purpose centrifuges, visit www.thermoscientific.com/centrifuge Thermo Scientific Sorvall Legend XT centrifuges:
Accelerate your research with increased capacity in the same bench space.
Moving science forward

60 Lab Manager Product Resource Guide 2010 www.labmanager.com
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cHilleRs
pRoducts in action
poRtaBle cooling at tHe point of use

Lab chillers remove heat from one object and transfer it to another, usually by means of a liquid. Thermo Fisher Scientific defines chillers as refrigerated recirculating liquid cooling system[s] consisting of a compressor, condenser, evaporator, pump, and temperature controller, all in one package. Chillers cool and maintain temperatures through one of three main methods. Compressor cycling, similar to thermostatic temperature control, maintains a desired temperature by turning the cooling engine on and off. The main disadvantages are difficulty achieving precise temperature control and compressor wear. By adding a heater to the return loop, the compressor remains constantly on. While less stressful to the chiller mechanism, heater cycling is energy-intensive. Hot-gas bypass is a sort of compromise, providing relative energy-efficiency and long compressor life. Regardless of the cooling method employed, chillers must release the heat they absorb through either an air- or water-cooled condenser. Because it releases heat to the atmosphere, air-cooled condensing works best with large rooms and small chillers. Large units in small rooms typically use the water-cooled method. Air-cooled chillers require less maintenance than water-cooled units, are simpler in construction, and consume slightly less power; water-cooled chiller condensers must be maintained periodically because of mineral buildup.
Chiller capacity is specified in tons, a misleading term that implies mass or weight. In this case, a ton is simply a measure of
BUCHI Corporation launches a new line of recirculating chillers

Reduced energy consumption and heat emission through full evaporation system integration.
19 Lukens Drive, Suite 400 New Castle, DE 19720 USA T 302 652-3000 F 302 652-8777 stern.m@buchi.com www.mybuchi.com
Most lab managers will be put off by the calculations required for sizing a chiller.
Chillers are technically not the same as circulators, although the terms are sometimes used interchangeably (and together). Circulators tend to be small, operate in a wide temperature range, and provide high temperature stability, although they have limited heat removal capability. Chillers are suitable for larger industrial applications and operate in a relatively narrow temperature range with modest (0.5C) stability, but provide much higher heat removal. A circulators reservoir can be used as a circulating bath, while a chillers cannot. Chillers are rated by the quantity of heat they can remove per unit of time, which depends on the heat characteristics of the application. Most lab managers will be put off by the calculations required for sizing a chiller. Luckily, vendors will perform the calculations provided the user knows the general parameters of heat removal required. A vendor can often help specify a chiller based solely on its intended application. heat capacity in BTUs. Twelve thousand BTUs equal one ton. Tabletop systems used mostly for laboratory processes are referred to as fractional chillers and are available in capacities of onequarter to one-third ton (3,000 and 4,000 BTUs, respectively). When purchasing a chiller, factors to consider include ambient operating temperature, desired process temperature range, temperature control tolerance, process fluid type, process fluid pumping volume, process fluid supply pressure, and most important, the amount of heat to be dissipated from the process.
October 2010 - Buchi, a leading provider of integrated lab-scale evaporation solutions and robust industrial evaporation equipment is proud to announce the launch of an all-new range of recirculating chillers designed to work seamlessly with a rotary evaporator and vacuum controller. The F-series chillers provide the final step to a fully integrated evaporation or distillation process. This new line of chillers, which connect to the rotary evaporator and can be controlled from the evaporation systems vacuum controller, includes: F-100 an entry level model which offers an attractive alternative to chilling with tap water F-105 smart control through vacuum controller with a cooling capacity of 500W at 15oC for use with a single rotary evaporator F-108 smart control through vacuum controller over a range of -10oC to +25oC with a cooling capacity of 800W at 15oC F-114 smart control through vacuum controller over a range of -10oC to +25oC with a cooling capacity of 1400W at 15oC F-125 a powerful industrial chiller with a cooling capacity of 2500W at 15oC and an integrated trolley for use with the Rotavapor R-220 SE industrial rotary evaporator Recirculating chillers that are not fully integrated with the entire evaporation system waste a lot of energy and generate heat
between the distillation runs because they do not shut off automatically, remarked Michael Stern, Director of Marketing at BUCHI Corporation. Buchis new F-series chillers actively save energy and reduce heat emissions when integrated with Buchi rotary evaporators and Buchi vacuum controllers, he added. In addition to their use with rotary evaporators, these new chillers are well suited for cooling applications with Buchis parallel evaporation, solvent extraction, and automated Kjeldahl products. For over 50 years, Buchi has been known as the market leader, inventor and innovator of lab instruments based on Evaporation and Vacuum technologies, and as the supplier of the Rotavapor products worldwide. In addition, BUCHI Corporation is a proven North American provider of spray dryers for pharmaceutical and food agglomeration and microencapsulation, Kjeldahl and solvent extraction equipment for environmental and food analysis, NIR spectroscopy instruments for pharmaceutical and food Quality Control, modular flash chromatography systems, and other related laboratory equipment. Headquartered in New Castle, Delaware, BUCHI Corporation is an affiliate of BUCHI Labortechnik AG (Flawil, Switzerland).
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NEW FOOD SAFETY RESOURCE CENTER NOW AVAILABLE AT LABSAFETY.COM
Make Lab Safety Supplys new Food Safety Resource Center your online source for safe food handling information. The Food Safety Resource Center features technical information, product information and articles relating to Food Safety. Safe food production demands incredibly high standards for safety, quality, sanitation, maintenance and handling. Lab Safety is devoted to helping you with Food Safety. Visit the Food Safety Resource Center today at www.labsafety.com/foodsafety/. Every lab needs a terrific resource and weve got it. Lab Safety Supplys Lab Supplies catalog is your best choice for quality lab supplies and equipment to keep your lab running smoothly, safely and efficiently. Call 1-800-356-0783 today to request your 1060+-page catalog. Food Safety continues to grow as an issue as both internal and external threats to our food supply rise. Companies are looking at ways to make sure they are protecting their processes and putting out the best products possible. There are many facets of food safety and food monitoring to keep every cog in the food machine running smoothly. Visit the Lab Safety Supply Food Safety Resource Center (www.labsafety.com/ foodsafety/) for the products and information essential to meet the challenges of producing first-class food products safely and efficiently. The government also wants to ensure they have the proper regulations and regulatory agencies in place to protect the American people from any external sources of harm to our food supply. The Food and Drug Administration (FDA) has a primary purpose to protect citizens against harmful, unsanitary or falsely labeled foods, drugs, cosmetics or therapeutic devices. The FDA publishes their regulations in the Code of Federal Regulations, CFR 21. The U.S. Department of Agriculture (USDA) assists agriculture in the production and distribution of high-quality food and fiber commodities. During production, many food products are tested to quality-control parameters. Temperature measurement is a key parameter. The standard danger zone for food is 40140 F. That is the range when pathogens are most likely to grow. Monitoring food temperatures assures your product will be the best it can be. Proper pH measurement not only affects the look, taste and quality of products, but maintaining a proper pH level is also a food safety issue. Created by Lab Safety Supply October 2010 A low pH reading of 4.6 will prevent the growth of deadly bacteria such as botulism in canned or preserved foods. Accurate monitoring of pH during the productions of these types of foods may be mandated by state or federal regulations. Plastic or metal contaminants getting into a product creates problems both from a health and from a public relations standpoint. Metal-detectable products provide quality assurance and help you find foreign objects in products before they reach your customers. Find out more about Food Safety topics by visiting www.labsafety.com/foodsafety/ today. In addition to the resource center, there are more than 270 EZFacts documents available at www.labsafety.com/refinfo, including: No. 500 Metal Detectable in the Food Industry No. 502 pH Electrodes No. 503 Food Defense No. 506 Who Governs the Food Chain No. 507 Temperature Measurement Instrumentation
pRoducts in action
Recently Released cHiLLeRS anD BatHS EchoTherm Model IC20XR Aqua Plus Series
Can freeze, chill or heat samples from -10C to 110C in assay plates, centrifuge tubes, vials and test tubes Features a digital display and control of temperature to 1C Includes data logger and RS232 interface to control unit by computer or to record data A variety of over 25 standard sample blocks are available
Four models available: digital and analog controlled Unstirred Baths, digital controlled Linear Shaking Baths, and safety protected Boiling Baths All models are available in a variety of sizes to meet a wide range of applications Feature easy-to-clean stainless steel tanks, drain taps and non-drip polycarbonate lids and bases Grant Instruments www.grantsci.com
401 S. Wright Rd. Janesville, WI 53546-8729 1-800-356-0783 www.labsafety.com
Torrey Pines Scientific www.torreypinesscientific.com
ThermoFlex 24000
Newest addition to the NESLAB ThermoFlex series Features a cooling capacity of 24,000 watts Includes water filters that can be changed while the unit is in operation Features an intuitive user interface for ease of operation Thermo Scientific www.thermoscientific.com
Designed for use with proprietary beads, which naturally hold items in place No need for racks, floats and bottleneck weights Users can safely incubate multi-well plates, Petri dishes and open-top samples Thermal uniformity is 0.5C at 37C and 1.0 at 65C with temperature range of 5C above ambient to 80C Lab Armor www.labarmor.com
Bead Bath
cHiLLeR anD BatH ManUfactUReRS

Cincinnati Sub-Zero Boekel Scientific Grant Instruments Julabo Legacy Chiller Systems PolyScience TECA Corporation TEK-TEMP Instruments Thermo Fisher Scientific Torrey Pines Scientific VWR International www.cszindustrial.com www.boekelsci.com www.grantsci.com www.julabo.com www.legacychillers.com www.polyscience.com www.thermoelectric.com www.tek-tempinstruments.com www.thermoscientific.com www.torreypinesscientific.com www.vwrsp.com
Forget the hassle of storage solutions with the IQ 160 Waterproof pH/mV/ Temp/ORP Meter because you can store the stainless steel probe dry! It requires no maintenance and, unlike delicate glass probes, you dont have to fret over possible breakage. It stands up to harsh use in difficult applications. Ask for No. 141821.
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clean RooM fuRnisHings

Recently Released cLean RooM fURniSHingS 7000 Silver Series Seating Series 19 Dessicator Cabinets
Can be purged to create a controlled environment for long-term storage Constructed with a heavy-gauge stainless steel outside shell and a stainless steel divider between each compartment Also available in polypropylene Clean Air Products www.cleanairproducts.com
Holding up to WoRKloads in classified space

Choice of clean room casework, or furniture, is one of the most important decisions made when setting up classified space. Cabinets and associated doors, hinges, handles, panels, benchtops, shelving, and vertical/horizontal surfaces must be compatible with the application and the clean rooms classification by Federal Standard 209E for airborne particulate cleanliness. Surfaces must be as easily cleaned as walls and floors, emit no particulate contaminants, and above all resist exposure to liquids and solids processed inside the room. Since the purpose of a clean room is to protect the environment from hazardous materials or sensitive materials from the environment and humans, or both, clean room casework must fulfill those missions and be environmentally invisible. Most clean room casework today is made from coated steel, stainless steel, and polypropylene. Polypropylene casework has been around for years, remaining a niche product due to its high cost, but Terry Thompson, polypropylene sales manager at NuAire (Plymouth, MN) says polypropylene is the material of choice for clean rooms that use corrosive acids or chemicals or that experience high humidity. Polypropylene is about 2.5 times as expensive as steel or wood casework and just slightly more expensive than stainless steel. As Thompson explains, Polypropylene is made from a petroleum product, so were at
Clean room casework must be environmentally invisible.

the mercy of the oil markets. But more important, a polypropylene cabinet needs a lot of handling during manufacturemuch more than stainless steel Polypropylene edges are sharp and must be smoothed and de-burred, then welded together. Clean room casework is normally specified by whoever plans the room, which is either an architect or a company engineer. Owners increasingly ask for modular casework, Thompson explains, because it provides versatility and changeover capability when a clean rooms mission changes. Outside design firms sometimes over specify for casework,
Thompson says, to cover all contingencies. In one instance where polypropylene casework was designated, Thompson called the owner to confirm that the application called for it. They didnt realize how expensive it was and wound up ordering a less costly alternative that suited their needs just as capably. If you can get by with metal casework, thats obviously the way to go. Hemco specializes in Class 1000 and Class 10,000 clean room furnishings and installations, a niche that Campbell describes as clean labs to distinguish them from higherclass semiconductor processing suites. Hemco has done Class 100 installations, but usually as subareas of Class 1000 rooms. Within that marketplace the company sells casework fashioned from welded steel coated with an epoxy powder coat finish. These structures are fabricated as easily as stainless steel but have much higher resistance to acids and moisture. They are also available for about onethird the cost of polypropylene and stainless.
Certified for Class 10 cleanrooms; Meets ANSI/BIFMA Standards Features heavy-duty chrome-plated back bar and springloaded hinge assembly Features easy-to-use pneumatic seat height adjustment and fully adjustable contoured back Includes sturdy five-legged polished aluminum base with standard glides or optional casters BEVCO www.bevco.com
Features an electropolished stainless steel work surface that eliminates dust accumulation Bottom shelf and frame are adjustable in two-inch increments Support structure virtually eliminates drumming Available with a bottom shelf, H-frame or 3-sided tubular frame Terra Universal
InterMetro Clean Room Tables
Airegard 301 Horizontal Laminar Flow Cabinet

Features a HEPEX Zero-Leak airflow system for consistent airflow and HEPA filter loading Features a work surface splash-back to protect the HEPA filter Includes a Minihellic pressure gauge, fluorescent lighting and an adjustable motor speed controller Can be customized with UV lamps, taller filters, wider work surfaces and stainless steel work surfaces NuAire
www.terrauniversal.com
www.nuaire.com
cLean RooM fURniSHing ManUfactUReRS

American Cleanroom Systems BEVCO CleanAir Solutions Clean Rooms West Gerbig Engineering Company HEMCO Liberty Industries PortaFab Sovella Terra Universal United Lab Equipment www.americancleanrooms.com www.bevco.com www.cleanroomspecialists.com www.cleanroomswest.com www.gerbig.com www.hemcocorp.com www.liberty-ind.com www.portafab.com www.sovella.us www.terrauniversal.com www.unitedlabequip.com
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fuMe Hoods
AirClean Systems Independence Ductless Fume Hood
As research and laboratories evolve, AirClean Systems continues to innovate with sophisticated, efficient fume containment solutions. Independence is the culmination of two decades of research and development in airflow design, gas-phase filtration, fume detection and hood control technologies. Drawing on feedback from thousands of customer applications, the Independence fume hood incorporates unique features not currently incorporated into other fume hoods.
pRoducts in action
It would be difficult to imagine a chemistry laboratory without at least one fume hood. Despite their ubiquity and the notion that they are not sexy lab products, a great deal of innovation has occurred in fume hoods during the last decade. Fume hoods are connected to a buildings heating, ventilating, and air conditioning (HVAC) system, which removes air through the hoods front panel. Wasting air by forcibly removing it is the most significant ongoing cost of fume hood operationgreater than the cost of acquiring and installing a hood, and much greater than the hoods operating costs.
coMModity laB appliances, But stRong on innovation

$5,000-$6,000 per year to operate. Low-flow systems are designed to operate at 60 cfm at all sash positions. Yet low flow is by no means universally acknowledged as an improvement. Alvin Heath, director of business development at ESCO Technologies (Hatboro, PA), says low-flow hoods challenge the traditional concept that higher inflow equates with better containment. Convincing end users to work with these hoods is an uphill task. Some notable features available on modern fume hoods include: improved ergonomics, making them easier to work in for long periods;
Fume hood prices are often bundled with furniture prices, and that makes it difficult for the end user to make informed decisions.
About ten years ago, the industry introduced the first low-flow fume hoods, which operated at lower face velocities than did traditional hoods. The standard used to be about 100 cfm; low-flow systems vent toxic gases just as well at 60 cfm, a 40 percent energy savings. Typical lab air entails costs of about $5/cfm/yr, so a typical old-style hood costs about intelligent sashes, which close when a motion sensor detects no movement in front of the hood for a given time period, reducing energy consumption; improved hood baffle designs, to allow for best airflow navigation; and digital airflow monitors, which can alert personnel when problems arise. Perhaps the most striking development is the
ductless fume hood, which recycles conditioned air. Users should be aware of problems that may crop up during specification and installation of fume hoods. Most new fume hood purchases are for new laboratories, according to Alvin Heath, director of business development at ESCO Technologies (Hatboro, PA). Traditionally, laboratory furniture suppliers provide the fume hoods as well. Although a few manufacture hoods that are standards-compliant, Heath says, many still construct fume hoods as though they were simple boxes. Fume hood prices are often bundled with furniture prices, and that makes it difficult for the end user to make informed decisions. Heath provides the following wish list for potential fume hood buyers: Local installation and support for ducting, controller, and exhaust blower
Raleigh, NC USA 919-255-3220 800-849-0472 info@aircleansystems.com www.aircleansystems.com
AirSafe TOUCH, the first touch-screen microprocessor controller with software written exclusively for use with ductless fume hoods, serves as the technology backbone for many features debuted by Independence. An engaging, userfriendly interface, AirSafe TOUCH allows the user to quickly access all controls while providing displays of vital data such as fume hood face velocity, gas levels and alarm status. Moving beyond the industry-standard metal oxide sensor, a PID (photo ionization detector) monitors the systems gas-phase filtration, providing gas saturation readings in parts-permillion. The filtration bed, fume hood exhaust and laboratory air are all monitored by the PID. User-defined alarms can be enabled for each sensor and monitoring location, ensuring the user is notified of any potential exposure. Carbon-based filtration, as found in most ductless fume hoods, has been in use for centuries. With the invention of Silconazyne, AirClean Systems has improved upon carbon filters by increasing the adsorption capability on a wider spectrum of commonly manipulated laboratory chemicals. Silconazyne has an improved efficacy for capture of polar organic solvents, nonpolar organic solvents, inorganic bases and inorganic acids. Filtered air is recirculated by ductless hoods, making it imperative that the filtration be capable of capturing chemicals used within the hood. Built into the Independence software is a chemical reference library containing more than 1,000 chemicals approved for use with Silconazyne. During factory QA/QC, the approved chemical application is programmed into Independence. In the event another chemical is to be added, the administrator
can updated the approved application through AirSafe TOUCH. The system then validates the new application against the installed filters and gas detection package to confirm compatibility. For years, fume hoods have been seen as the laboratorys biggest consumer of energy. Ductless hoods such as Independence recirculate clean, filtered air, limiting the amount of energy spent removing and reconditioning air within the laboratory. To further reduce energy use, lack of operator interaction activates the Independence standby mode. Once in standby mode, energy consumption is reduced to a minimum while maintaining operator safety. Independence provides three methods of airflow monitoring and control. The most popular is automatic mode, where a user preset value is entered into the microprocessor and face velocity is maintained automatically during the course of operation. Manual mode allows the end user to increase or decrease blower speed at their discretion while high/low mode sets the blower to one of two speeds based on sash position. For labs with multiple hood users, Independence includes the ability to have eight unique user profiles with definable operation perimeters for each. At the owners or administrators discretion, an individual user can be given access to certain features. Examples of definable user access include enabling burning gas, modifying alarm settings, and changing blower modes. Engineered as a platform capable of evolving as technology and market demands change, Independence is the foundation for all future ductless fume hood solutions.
Appropriate safety certifications Construction materials for specific application, for example, polymer inner liners for corrosive acids, ceramic work tops for high temperatures Local references for the supplier/ installer Aesthetics and cost
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fuMe Hoods
Captair Flex Ductless Mobile Fume Hoods with Modular Filtration Column
From the people who brought you the energy saving GreenFumeHood, ERLAB, the one and only inventor of the ductless filtering fume hood and worldwide leader since 1968 innovates once again with the low cost Captair Flex Technology, an all in one filtration fume hood design configurable at will for use in chemistry, biochemistry, pharmacology, forensics, histology/pathology and more. New modular filtration column can handle liquids and powders individually or at the same time. Innovative liquid seal technology insures filtration integrity for both molecular and HEPA filters. Unique design allows for single or double back-up safety filtration to comply with the AFNOR NFX 15-211 Class I and II safety standard.
pRoducts in action
Recently Released fUMe HooDS UniFlow SE Constant Volume Mobile EDU Ductless
Features a unitized flame-retardant composite resin construction for chemical and corrosion resistance Angled picture-framed sash opening has an aerodynamic air foil for uniform air flow entry into the fume chamber Surfaces are glass smooth for ease of cleaning with excellent reflectivity HEMCO www.HEMCOcorp.com
Completely self-contained and provides all around visibility Features a Multi-Layered EDU Filter with 99.9% filtration efficiency Base is mounted in large heavy-duty wheels for ease of transport Exceeds OSHA, ANSI, BSI and AFNOR Safety Standards Air Science USA www.airscience.com
388 Newburyport Turnpike Rowley, MA 01969 1-800-964-4434 www.captair.com
Available in 4, 5, 6 and 8 sizes Operates at a broad range of face velocities from 60 to 1,000 fpm Sash handle, air foil, upper dilution air supply and rear downflow baffle contribute to horizontal airflow patterns to reduce concentrations of contaminants Labconco www.labconco.com
Protector XStream
Face velocity at 100 fpm ensures containment of fumes An alarm will alert the operator when the airflow falls to an unacceptable level The work area features a removable spillage tray which can be easily cleaned Main filter can be chosen from 14 different types of carbon
Purair Advanced Ductless
Fume Hood Safety & Flexibility Like never before

Captair solutions are designed with safety in mind. The Captair Flex filtration technology is based on the proven principal of molecular adsorption; the toxins emitted in the workstation are adsorbed by the activated carbon within the filtration column and captured keeping the user and the environment safe. The Flex also has a unique modular filtration design which allows the hood to handle liquids and powders individually or at the same time. This interchangeability of the filters allows the filtration column to be configured specifically for the applications carried out within the enclosure. The containment and filtration effectiveness of the Captair Flex, make this shared protective equipment a reliable, economical, flexible and environmentally-friendly solution.
ventilation system for air supply and extraction as required by ducted systems. A single electrical outlet is all you need to run the Captair Flex fume hood. It can be installed at any time, without complex planning. 3) Easily transport and instantly use your fume hood Captair solutions may be moved from one location to another within the same laboratory according to protection needs. They can be easily relocated without affecting the air balance of the equipment 4) Protect the environment Free of any ducted airflow system, Captair solutions eliminate the direct emission of pollutants into the atmosphere and help to protect the environment. They also avoid the pollution generated as a result of the energy needed to run the airflow systems of traditional ducted fume hoods.
Air Science USA www.airscience.com
fUMe HooD ManUfactUReRS

Air Science AirClean Systems CLEATECH Erlab ESCO Flow Sciences Genie Scientific Hanson Lab Furniture HEMCO ISEC Lab Synergy Labconco www.air-science.com www.aircleansystems.com www.cleatech.com www.greenfumehood.com www.escoglobal.com www.flowsciences.com www.geniescientific.com www.hansonlab.com www.hemcocorp.com www.isecinc.com www.labsynergy.com www.labconco.com LM Air Technology Misonix Mott Manufacturing mottLAB NuAire Salare Sentry Air Systems Terra Universal TFI/Inline Design The Baker Company www.lmairtech.com www.misonix.com www.mott.ca www.mottlab.com www.nuaire.com www.salareinc.com www.sentryair.com www.terrauniversal.com www.tfiinlinedesign.net www.bakerco.com
RDM Industrial Products www.labspacesolutions.com
The Captair Flex can:

1) Save on energy costs in your lab The air balance necessary to run ducted systems results in high energy consumption. A Captair solution eliminates the energy costs related to systems for extracting and supplying conditioned air. It is able to keep operating costs low, even when the cost related to filter replacement is taken into account. 2) Eliminate installation costs associated with fume hoods Implementing a Captair Flex is simple and quick. It does not involve the installation of a
Captair Flex, designed to protect the user, the environment and your budget.
Germfree Laboratories www.germfree.com
Thermo Fisher Scientific www.thermo.com
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glove Boxes
Recently Released gLove BoxeS
veRsatile, on-deMand isolation oR containMent

Glove boxes are completely closed compartments ranging in size from a few cubic feet to several hundred cubic feet. They differ from other safety enclosures in two ways: users can introduce articles into glove boxes and manipulate them inside through ports fitted with gloves, and glove boxes typically use a specialized atmosphere.
Although glove boxes are most often associated with biology, all scientific and engineering disciplines use glove boxes for one application or another. They are most commonly used when a process or operation requires low humidity or low oxygen levels, or when either the product/ process must be protected from the lab environment or the operator
the cleaner it is on the inside relative to the outside, the greater the equilibrium difference.
Glove boxes consist of the main chamber, two glove ports, and an airlocked antechamber for introducing labware and materials into the box. Opening the antechamber without taking preventive measures will introduce ambient atmosphere into the working chamber. This is dealt with by providing vacuum-assisted purging with the desired atmosphere. Sensitive applications will often add sensors for oxygen and/or water, with some type of scavenger mechanism to achieve ppm concentrations of those species. In regulated industries, the purge cycle is software-controlled and documented to ensure that materials are handled to specification. needs protection from the process or operation. One often hears the terms isolation and containment with respect to glove boxes. Isolation is meant to protect the product, while containment refers to protecting the operator and/or environment. Isolation normally involves positive pressure, while containment operates under negative pressure. A quick online shopping search for glove box leads to a dizzying array of devices ranging in price from $411 to $50,000, from simple plastic boxes to sophisticated mini clean rooms that meet ISO sterility requirements. Glove boxes for regulated industries will almost always
include pressure gauges, validatable oxygen and moisture monitoring, and built-in data transmission. According to Bob Applequist, product manager at Labconco (Kansas City, MO), price is the principal factor affecting most glove box purchase decisions. Most of our customers have to watch their budgets. Any glove box can achieve very low oxygen or moisture readings, he explains, but the cleaner it is on the inside relative to the outside, the greater the equilibrium difference. Without taking additional measures, he says, oxygen and moisture can creep up to 10 percent in 10 minutes. Customers, he says, often over specify for oxygen and moisture removal based not on actual data but on perception or assumption. They know they want low oxygen and moisture, but when you ask them how low, 95 percent dont really know. Maintaining 1 ppm levels 24 hours a day will be quite expensive, particularly with respect to scavenger systems.
Features a HEPA inlet filter for a particulate-free internal environment Closing two internal valves creates a controlled atmosphere that can be pressurized up to 5 inches water gauge achieving a leak-tight inert atmosphere Physical barrier and filtration system protects user from exposure to hazardous materials Labconco www.labconco.com
Protector
Complete glovebox system with gas purification; for users with limited space Can be supplied with a stand, or fits on any standard benchtop Capable of producing a < 1 ppm oxygen- and moisture-free environment Features a one-piece radius corner construction for easy cleaning
LCPW Series
LC Technology Solutions www.lctechinc.com
Provides a < 1 ppm O2 and H2O Inert Atmosphere Includes a single-column gas purification system as standard (dual-column available) Expansion features include additional antechambers for thru flow work processes, cold storage freezers, process vacuum ovens and furnaces Innovative Technology www.gloveboxes.com
PureLab HE Series
Features smooth, ultra-clean surfaces to eliminate germ traps and simplify cleaning and disinfection Continuous-seam interiors create a hermetically sealed chamber Each chamber includes a pair of 10-inch diameter glove ports (twin models include two pairs) Terra Universal www.terrauniversal.com
BioSafe
gLove Box ManUfactUReRS

Coy Laboratory Products Innovative Technology Labconco LABREPCO La Calhne Getinge LC Technology Solutions MBraun USA MTI Corporation www.coylab.com www.gloveboxes.com www.labconco.com www.labrepco.com www.lacalhene.com www.lctechinc.com www.mbraunusa.com www.mtixtl.com NuAire Plas-Labs Plastic Concepts Terra Universal The Baker Company Walker Barrier Systems www.nuaire.com www.plas-labs.com www.plastic-concepts.com www.terrauniversal.com www.bakerco.com www.walkerbarrier.com
Vacuum Atmospheres Company www.vac-atm.com
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incuBatoRs
CO2 Incubators 180 C Sterilization Cycle Protects Cultures
Contamination in culture experiments is so widespread that many labs accept a low level of contamination as a norm. But this pernicious problem costs laboratories a lot of money, is responsible for lost time, can ruin experiments and invalidate research, and in some cases is even responsible for the loss of invaluable or irreplaceable cell lines. There are a number of features exclusive to CO2 incubators from Binder, Inc., of Great River, New York, most prominently a 180 C hot air sterilization cycle, that protect cultures and that are not found in other incubators. Here is a look at what these features mean for researchers.
pRoducts in action
BetteR teMpeRatuRe contRol

Laboratory incubators are used to grow and maintain cell cultures and are available in a variety of sizes and types. They are divided into two main categories: gassed incubators (CO2 incubators) and non gassed or microbiological incubators. CO2 incubators are mainly used for cell culture and provide control over factors such as temperature, CO2 for maintaining proper pH levels, and humidity, all of which affect cell growth. CO2 incubators are typically heated to 37C and maintain 95% relative humidity and a CO2 level of 5 percent. Microbiological incubators are essentially temperaturecontrolled ovens that work within the biological range of 5C to 70C and are mostly used for growing and storing bacterial cultures. Most incubator units are water-jacketed, air-jacketed or use direct heat to maintain the temperature around the culture chamber. Incubators can be used in a wide variety of applications including cell culture, biochemical studies, hematological studies, pharmaceutical and food processing. Shaking incubators are often used for cell aeration and solubility studies. Refrigerated Biochemical Oxygen Demand (BOD) incubators, with a temperature range of 20C degrees to 45C below ambient, are commonly used in insect and plant studies, fermentation studies and bacterial culturing. The cell culture market today is thriving predominantly due to new applications in areas like stem cell research and hence there is more potential for growth in these products, says Douglas Wernerspach, global product manager, CO2 incubation at Thermo Fisher Scientific.
interiors made of solid copper components are also gaining interest. Solid copper or 100% pure copper is naturally antimicrobial and for the first time the U.S. EPA has also recognized copper, a nonchemical, as an effective antimicrobial agent, says Wernerspach. This has led to a
Toll Free 866 885 9794 sales@binder-world.us www.binder-world.us
Ultimately you want to go with something that best meets your requirements.
Many manufacturers are working toward addressing some of the common challenges associated with culturing cells, the most important of which is reducing aerial contamination. A number of incubators offer a high-temperature decontamination cycle that works much like a self-cleaning oven. With the press of a button, the customer can heat-sterilize the incubator and get rid of any decontaminants or hazardous spills, says Wernerspach. This option also eliminates the need to take apart individual components for autoclaving. Besides units that can be activated when needed, there are also continuous contamination prevention units that work all the time and do not have to be initiated manually. One technology uses HEPA filtration to continuously cycle the air and remove airborne particulates and contaminants. Incubators with number of companies developing copper-based products. Incubators also come with options that can further increase user ease and convenience, including touch screens, data storage, removable shelves and programmable alarms. At the end of the day what customers really care about is having a reliable unit in which to grow their cells. Hence, the lab environment, the application and the customers comfort level with the technology is what plays a big role in the selection of the equipment. Ultimately you want to go with something that best meets your requirements, says Wernerspach.
Complete sterilization is the only way to eliminate contamination from a CO2 incubator, and the 180 C hot air cycle used in the BINDER incubator exceeds all international standards. Heres how it works: At the end of a given run, the user wipes up any spills that may have occurred, empties the water pan and replaces it, and, with the shelves still in place, closes the door and pushes three buttons (three are used to prevent accidental start-up) to start the cycle. When 9-1/2 hours have passed, the incubator is completely sterilized and ready for the next batch of cells. Leaving the shelves in the incubator during the sterilization procedure means that there is no reassembly. This results in less handling, and it also means that the shelves are sterilized along with the rest of the interior. These incubators feature a one-piece, weld-free interior with rounded corners, eliminating spots in which contaminants can lodge. The chamber interior is guaranteed to be condensation free, with excess moisture re-condensing in the water pan. BINDER CO2 incubators do not use HEPA filters, which harbor the very pathogens they trap. Filter replacement and disposal costs are eliminated. Air within the units APT.LINE preheating chamber is blown over heating elements and around the culture compartment, providing uniform temperatures and fast recovery times. Heating elements never make contact with the units walls, eliminating hot spots. A double-latch locking system tightly seals doors and facilitates quick return to set point for temperature and CO2. The growth of healthy cells in all locations is dependent on environmental conditions remaining
constant throughout the incubator over the length of the culture process. How stability is accomplished, monitored and maintained is integral to unit performance. If CO2 percentages are out of balance, culture pH changes, resulting in an environment that is either too acidic, or one in which ammonia can form, either of which is deadly to cells. Figure 1 is a graph of a 5% CO2 set point showing virtually drift-free conditions over a 24-hour period. CO2 was monitored for the test shown using nine sensors placed throughout the chamber. These infrared sensors are dedicated to measurement of CO2, operate independently of other gases, and are not affected by humidity levels. There performance is critical because CO2 percentages that are out of balance can lead to culture pH changes and result in an environment that is either to acidic, or one in which ammonia can form, either of which is deadly to cells. BINDERs quality control process adheres to the German industrial, or DIN, standard. After heating a newly made incubator to steady state over a two-hour period, temperature is sampled six times a minute at 27 points throughout the chamber. What this means is that a scientist using a BINDER CO2 incubator can be confident in the units performance and the environment provided for their cells. Taken together, the anti-contamination features, performance features and demanding quality control standards mean that the scientist culturing cells can be assured that Binder Inc. incubators provide the best conditions for their success.
The Binder CB 53 CO2 incubator.
A 5% CO2 set point is virtually drift-free over 24 hours.
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NuAire AutoFlow Incubators
All models feature a fanless design, eliminating a possible source of contamination Employs six-sided, direct heating with gentle convection circulation of chamber atmosphere R-Series models are available in 48-liter and 170-liter capacities; S-Series models are available in 14-, 48- and 170-liter capacities New Brunswick Scientific www.nbsc.com
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Recently Released incUBatoRS Oasis GALAXY Direct Heat
Experience Constant Contamination Control

NuAire offers both Direct Heat and Water Jacket CO2 AutoFlow Incubators assuring the highest level of performance and dependability for optimum growth conditions. An environment is provided for storage and preservation of embryos, gametes and animal tissue cell cultures at or near body temperature. Designed to provide precise CO2, temperature, and relative humidity
6 cu. ft. model features infrared CO2 control, direct heat and high relative humidity Exterior is powder-coated with ISOCIDE antimicrobial paint to protect against contamination In the event of contamination, an overnight sanitization cycle can be activated
Best Products Best Performance Best Protection
2100 Fernbrook Lane Plymouth, MN 55447 www.nuaire.com/co2-incubators
Caron Products www.caronproducts.com
Series includes 6.0 cu. ft and 12.0 cu. ft. models Features inCu safe copper enriched stainless steel interior surfaces to protect against cross contamination Optional SafeCell UV contamination control system minimizes decontamination interruptions Direct Heat and Air Jacket surrounds inner walls with natural convection airflow
CytoGrow GLP Series
Features triple-wall cabinet construction for optimal temperature uniformity Includes a validatable in-chamber HEPA filtration system to maintain Class 100 air quality Features a CFC-free foam insulated outer door Extra options include available oxygen control, relative humidity monitoring and antimicrobial copper interiors Thermo Fisher Scientific www.thermoscientific.com
Forma Series II 3110
control promoting cell culture growth. Control mechanisms are built in to prevent contamination within the growth chamber. Programmable digital solid-state infrared [IR] CO2 Sensor provides a stable, drift-free output, making necessary corrections to the chamber environment and supports faster recovery times. The Closed Loop HEPA filtration system creates an ISO Class 5 air quality inside the chamber, creating Constant Contamination Control (C3) for a dependable contamination-free environment.
The DH AutoFlow NU-5100 CO2 Incubator is the perfect workhorse incubator for research where there are multiple door openings per day. Designed to provide a sterile, constant temperature, constant CO2 level and naturally humidified atmosphere for optimum growth of tissue cell cultures and other organisms requiring this precise environment. The growth environment is applied equally to all microorganisms within the chamber. The 99.99% HEPA filtration system ensures ISO Class 5 clean air quality inside the
is achieved. The exterior chamber walls are lined with high tech R5 insulation to provide excellent temperature stability and energy efficiency. A chamber capacity of 188 liters provides more usable space at a lower price per liter than other incubators. The coved corner interior makes chemical disinfection easy. The NU-5500 is the ideal incubator for optimum tissue cell culture growth and an array of clinical applications. NU-5510 DHD AutoFlow Direct Heat CO2 Incubator was designed for such applications as AIDS Research. It provides dual sterilization cycles; +95C humidified decontamination and a +145C dry sterilization eliminating all contamination. Perfect for cell line changes and/or working with hazardous agents. Designed with an external IR Sensor, there is nothing to be removed from the chamber prior to heat sterilization; which makes this the easiest incubator to use for delicate applications. As with all NuAire AutoFlow Incubators, the NU-5510 offers Password Protection capabilities to prevent users who are unauthorized to change the setpoints (CO2%, Temp) or running conditions of the incubator.
SANYO North America www.us.sanyo.com
NuAire AutoFlow CO2 Incubators
incUBatoR ManUfactUReRS
BINDER Boekel Scientific Carbolite CARON Products Darwin Chambers Grant Instruments Hach Company Jeio Tech Labnet International www.binder-oven.us www.boekelsci.com www.carbolite.com www.caronproducts.com www.darwinchambers.com www.grantsci.com www.hach.com www.jeiotech.com www.labnetlink.com NuAire Sanyo Sheldon Manufacturing So-Low Thermo Fisher Scientific Torrey Pines Scientific UVP Wheaton Yamato Scientific
Setting the StandardS for demanding environmentS
www.nuaire.com
www.sanyobiomedical.com www.shellab.com www.so-low.com www.thermoscientific.com www.torreypinesscientific.com www.uvp.com www.wheatonsci.com www.yamato-usa.com
New Brunswick Scientific www.nbsc.com
NuAires AutoFlow CO2 Water Jacketed Incubators maintain up to 98% Relative Humidity (RH) reducing desiccation inside the chamber, therefore, less media is needed and less handling of cultures is required. This reduces the chance for contamination. The Incubator jacket creates a gentle insulator surrounding the innerchamber providing unsurpassed temperature stability. AutoFlow Water Jacket Incubators have been designed to provide a reliable controlled in vitro environment for optimum tissue cell culture growth. The chamber also provides an environment for the storage and preservation of embryos, gametes and animal tissue cell cultures at on near body temperature.
work chamber, which produces Constant Contamination Control (C3). With a 4.4 cubic feet [130 liter] chamber capacity and an overall height of 31 inches, this compact incubator fits under most standard lab benches, saving lab space. NuAires NU-5500 DH AutoFlow Direct Heat CO2 Incubators offer a unique HEPA Filtration System that converts this incubator into a miniature Clean Room, within 4 5 minutes after door closure ISO Class 5 clean air conditions
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Waters NuGenesis Scientific Data Management System
The ability to collect, process, and distribute scientific information is fundamental to the long-term success of science-driven organizations. Waters NuGenesis Scientific Data Management System (SDMS) has a proven track record of optimizing the management of scientific data for hundreds of companies around the world. With SDMS, laboratories are able to automatically capture, secure, access, and disseminate information from a wide variety of analytical technologies. The instant accessibility of this information has resulted in stronger collaboration, more efficient operations, better decision making, and ultimately, accelerated product development and delivery for these world class organizations.
pRoducts in action
a pRoduct categoRy in tRansition

LIMS products vary from software for small laboratories to systems for enterprise-class distribution, where large implementations can cost millions of dollars and encompass licensing, training, validation and other required services. As with other software products, there are many ways to implement and purchase a LIMS, from boxed software to commercial licenses for COTS (Commercial Off-the- Shelf) to open source to SaaS (Software as a Service). It is important to note that a LIMS and an ELN (Electronic Laboratory Notebook) are not the same thing. An ELN is a literal replacement for your paper laboratory notebook. As such, it is a place to enter and keep your laboratory notes and get signoff; you use it just as you would use a paper notebook. The confusion between LIMS and ELN occurs partly because there is some overlap between them. Additional confusion arises now that some LIMS include ELN features, some ELNs contain LIMS features, and some products combine LIMS and ELN. Beyond the LIMS/ELN question, there are yet other products that sound like they might be a LIMS but have different names. There are still other products that are called LIMS but do not appear to be like anything that has just been described. Selecting and buying a LIMS takes effort and time in advance of the actual purchase for the following reasons: 1. The functions and distinguishing factors among available products are always changing. 2. LIMS is in a period of especially high transition as LIMS and ELN software continue to converge. Things might become even more confusing before this period is over. 3. Even before LIMS, ELNs and other products took on some of the functions of each other. Now the types of LIMS available are somewhat overwhelming. There are Environmental LIMS, generalpurpose LIMS, Web-only LIMS, PC-only LIMS, R&D-focused LIMS, QC-focused LIMS and Forensic LIMSto name just a few. a fully integrated Commercial Off-the-Shelf (COTS) solution available either from one solution provider or as a best-of-breed integrated solution. At Thermo Fisher Scientific, where an integration plan is used to turn specialized products into a single solution, Informatics marketing director, Susan Najjar, says, from a usability perspective, if users are working with a well-integrated solution, the merging of these technologies becomes irrelevant. What all this means is yet more choices to consider. But even though more choices may make the selection process lengthier, there is an increased likelihood that the best solution for your laboratory will be out there and readily available.
34 Maple Street Milford, MA 01757 Tel: 508 478 2000, 800 252 HPLC www.waters.com
The GMP-ELN and LIMS layers contain very different functionality.

A good idea might be to ignore the labels and look closely for the features that meet your needs. Ken Rapp of VelQuest, whose product combines LIMS and ELN features, explains, The GMP-ELN and LIMS layers contain very different functionality. However, market pressures are forcing a convergence of the requirements into
Waters NuGenesis Scientific Data Management System (SDMS) provides laboratories with an effective foundation for scientific information management by making data easier to access, facilitating compliance with regulatory requirements, and aiding in the administration of intellectual property throughout its lifecycle. NuGenesis SDMS automatically imports diverse data, generated by instruments, scientists, and outside sources into a centralized data warehouse. This information can easily be searched, communicated, and shared among scientists and team members collaboratively delivering measurable gains in time to market and employee efficiency while reducing cost and risk. NuGenesis SDMS is an application-independent software and database platform that you can use to create a common electronic repository for scientific information throughout your company from the raw data generated by instruments to project documents and electronic laboratory notebook reports, spreadsheets, summary documents, and presentations created by researchers.
NuGenesis SDMS technology scouts all servers, projects, and data types for all new content and then automatically extracts metadata (data about the data) during the capture process. This process builds a database catalog, which gives you the ability to quickly and easily perform searches across servers, projects, and data types.
exporting sources. It can work independently or in conjunction with existing IT systems such as LIMS, ERP, and electronic laboratory notebooks. Fully configurable, the SDMS platform scales from a single lab to the global enterprise without major modifications to your existing IT infrastructure. NuGenesis SDMS also includes SDMS Vision Publisher, an analytical electronic laboratory notebook (ELN) that allows laboratories to document scientific observations, control procedures, and easily find and collaborate on scientific results. SDMS Vision Publisher optimizes the utilization of information collected and cataloged by the NuGenesis SDMS platform. With an open and flexible design, Vision Publisher easily adapts to fit and support existing free-flow or prescribed workflows in your lab. SDMS Vision Publisher includes the SDMS Intelligent Procedure Manager, a workflow software package designed to guide the laboratory analyst through a routine, comprehensive method standard operating procedure (SOP) and integrate results with a chromatography data system.
Sophisticated data mining becomes much easier: search, retrieve, and view information according to a variety of data types, and restore results to their original applications. NuGenesis SDMS provides a single interface to a wide variety of instruments and data
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RURO introduces
the most comprehensive laboratory information management solution with LIMS 24/7. Single and secure lab data repository Lab processes automation Unlimited workflow scenarios Data mining and knowledge discovery 100% Web based Install once, access from anywhere Always up and running 24 / 7 / 365 State of the art architecture and design LIMS 24/7 meets CFR, GLP, ISO, CLIA and HIPAA standards providing you, your lab, and your clients with the best laboratory management possible.
pRoducts in action
Recently Released liMs
Matrix Gemini version 5.2.7

Compatible with Windows 7 Features a configuration wizard, allowing free choice of screen and menu designs, customer specific tables/ modules, and multiple sample numbering systems Assists with audit trails, time and date stamping of all actions, version control of all reference data, and helps with 21CFR Part 11 compliance Autoscribe
Compound Received data can be displayed in micrograms and milligrams in Dosing Data report Treatment concentrations are displayed to four decimal places on all screens The order preferred by each user in the Pooling Splitting screen is now saved on a user-by-user basis Importing WBA data from MCID analysis software is much easier LabLogic Systems www.lablogic.com
Debra 5.7.7
321 Ballenger Center Dr., Suite 100 Frederick, MD 21703 USA sales@ruro.com 1-888-881-RURO (7876) www.RURO.com
www.autoscribelims.com
LIMS-on-Demand Exemplar Version 4.0
Improvements in performance and scalability so a single server instance can now support several hundred concurrent users Includes enhancements to freezer management with support for configurable freezers and easy viewing of samples in shelves/racks/boxes Features a configurable data model and user interface with zero programming required Sapio Sciences www.sapiosciences.com
Software-as-a-Service LIMS; no need for costly installation and setup costs Users can securely and easily create workflows, map sample lifecycles and generate automatic updates from any standard Internet connection and through any web browser Enables connectivity with a variety of instruments
Recently, the demand for low-cost DNA sequencing has driven the development of high-throughput sequencing technologies that are based on several parallel instances of the sequencing process. This approach produces thousands or millions of DNA sequences at once, followed by algorithmic analysis and alignment of the data. The challenges of this approach are associated with handling massive amounts of the generated information. Next generation high-throughput DNA sequencing technologies are widely used by the scientists who embark on a monumental task of deciphering genetic information from a statistically significant population group. With the emergence of Epigenetics as one of the frontiers of biological science, the researchers face even greater challenges of piecing together genetic information and DNA-protein interactions. These research projects are insurmountable without a flexible laboratory data management system capable of storing, analyzing and sorting the data generated as a result of several experimental work-flows. In order to communicate results, manage processes at various stages or make common decisions, a software like LIMS 24/7 used in all labs could help to coordinate the work. The goal is to retain result achievement, elaboration, further decision and final review directly at the workplace. Indeed, all laboratory users can login and use this software from any-
where on the network. Projects are organized by type, subtype, priority and other criteria and all the related information is recorded within such projects. Moreover, instruments can send data directly to LIMS that is configured to guarantee the traceability of the work. With LIMS it is possible to save time and money, and to improve rate and productivity. LIMS 24/7 is an easy to use yet provides powerful analysis tool for streamlining the overwhelming sequencing process. It is an excellent solution for data management and sharing that can handle complex ChiP-Seq data including collaborators, donors, cohorts, antibodies, samples, DNA libraries, raw ChiPSeq data files, and analysis results. Powerful data mining capabilities help lab managers to make decisions on the next steps in the process, find bottle-necks and track performance. RURO is making genomic data easier to share, search and archive. We have presented one possible application of LIMS 24/7 in sequencing, but this software could also be successfully employed for the management of clinical trials, preclinical and animal research, drug discovery, microarray, genotyping, gene expression, biobanks and proteomics labs.
Thermo Fisher Scientific www.limsondemand.com
LiMS ManUfactUReRS
Agilent Technologies Aurora Systems Bruker ChemSW ChemWare Computing Solutions H&A Scientific LabLogic Systems Labtronics www.agilent.com www.visualab.com www.bruker.com www.chemsw.com www.chemware.com www.labsoftlims.com www.hascientific.com www.lablogic.com www.labtronics.com LabVantage Solutions LabWare Novatek International RURO Sapio Sciences STARLIMS Symyx/Accelrys Velquest www.labvantage.com www.labware.com www.ntint.com www.ruro.com www.sapiosciences.com www.starlims.com www.symyx.com www.velquest.com
Thermo Scientific Informatics www.labsystems.com
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New Brunswick Ultra Low Freezers.
New Brunswick Scientific, world renown shaker company, are also producers of high quality ULT freezers. These freezers are now being distributed in the US and Canada by Eppendorf North America. Carrying the same high quality, innovation, design, reputation and heritage as Eppendorf, NBS products carry on their legacy while creating a new one, together with Eppendorf. For the past 25 years, NBS have been offering ULTs that are environmentally friendly and exceptionally energy efficient.
pRoducts in action
HoW loW can you go?

Low-temperature laboratory freezers can achieve temperatures of about -40 C or lower. Kitchen freezers, by contrast, operate to about -20 C. The temperature sweet spot for lab freezers is about -85 C. Mechanical (compressor-based) freezers reach the -40 to -50 C range with a single compressor. A second compressor is required to cool below that point. To achieve energy efficiency and extend the life of the compressors, the low-stage compressor turns off automatically when the set-point temperature is reached, while the high-stage compressor remains on continuously. and data storage, alarms, quality/ configuration of the inner and outer door closures, vacuum release that permits reopening a freezer immediately after door closure, temperature uniformity, and temperature recovery after opening. Where floor space is at a premium, users can opt for vacuuminsulated panels, which take up less room than polyurethane insulation. Vacuum insulation used to be a rather expensive option, but it has come down in price, Richerson says. Another cold-conserving feature is double outer doors or double inner doors to minimize the loss of cold air. High-end freezers should maintain uniform temperature throughout the unit and recover quickly from openings. Opening the door of an ultralow-temperature freezer introduces warm, moist air that causes the device to work harder to retain its set-point temperature and causes condensation and freezing of water vapor inside the unit. The speed at which a freezer recovers from temperature excursions is a function of BTU reserve capacity, which, according to Thermo Fisher Scientific, is defined as a measure of a freezers ability to maintain a cold temperature across the entire cabinet in the presence of a heat load. Higher BTU reserve is better. Concerns with carbon footprint are spurring innovations in lowtemperature refrigeration. Among the trends are alternative cooling mechanisms such as liquid nitrogen, which boils at -196C. Cryogenic nitrogen is readily available and relatively inexpensive and remains liquid for extended periods, provided its container is insulated. A liquid nitrogen freezer does not use a compressor, and electrical consumption is less than for conventional freezers. But while the acquisition costs are comparable, ongoing costs for liquid nitrogen are higher than for the electricity it takes to run a mechanical freezer. Purchasers of low-temperature laboratory freezers can select from numerous options and temperature ranges, but as Chris Wilkes, director of product management for cold storage at Thermo Fisher Scientific (Asheville, NC) notes, Once you get below -135 C, there is not a huge benefit to getting colder.
Once you get below -135 C, there is not a huge benefit to getting colder.
Some systems using exotic refrigerants can get much lower than -85 C, explains Buckner Richerson, VP of international sales at NuAire (Plymouth, MN). For example, ultra-low-temperature freezers reach temperatures of -150 C, which is useful for storing bone marrow for medical procedures. But these are quite expensive, and not too many are sold, Richerson said. Factors to consider when purchasing include communications
The New Brunswick freezer lineup consists of two levels, Innova and Premium, that share many green features. Each freezer line is equipped with the same two-stage, high-efficiency cascade refrigeration system from Embraco, each fine tuned for maximum performance by model. This allows every unit to run shorter cycle times further reducing energy usage for a greener product. All NBS ULTs are CFC- and HCFC-free to reduce emissions of greenhouse gasses and minimize the depletion of the ozone layer- plus every unit is 95% recyclable!
capacity without increasing footprint. Other features unique to the Innova line include new gasketed and insulated inner doors with ice proof latches that lift off without tools for easier cleaning and reduce cold air loss when other compartments are accessed. Internal voltage stabilizers are also standard on the Innova line with Buck/Boost.
Premium Freezers:
Are conventionally filled with polyurethane foam insulation. These cost effective alternatives to the Innova line do not have a built-in voltage stabilizer or vacuum insulation paneling however, still retains many of the same quality features of the Innova line such as the easy to read LED display with SMART PLUS controller, Heated Vent plunger to relieve vacuum formation and password protection to prevent unauthorized changes to the freezer settings. The Premium line offers Five models, three large upright models the U410, U570 and U700 all offered in both 120 or 208/220volts. Each Premium upright utilizes five compartments with four adjustable height shelves. Two chest models are available, the C340 and C660 also with insulated inner lids.
Innova Freezers:
The Innova line has the widest capacity range from the smallest under the counter at 3.6 cubic feet to the largest at 26.9cu.ft. A total of six space-saving Innova models are available from stock for quick delivery. Included in the six models, there are two unique upright sizes which include the U101 under the counter and ultra-slim U360 with advanced LCD control panel. Two large capacity uprights for easy access to samples choose between the U535 and U725 each with three compartments and two adjustable height shelves to store any sized rack. Energy efficient, quiet running and dependable all Innova freezers use Vacuum Insulation Paneling technology to reduce wall thickness resulting in larger storage
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Helmer Introduces i.C Monitoring for the FutureNow
Helmer introduces the New i.C3 which offers constant temperature monitoring and multiple information logs with historical information on all i.Series products. The i.C3 is icon driven, featuring a 7 full-color touchscreen door mounted at eyelevel! An interactive temperature graph, home screen customization, password protection, and temperature download capabilities are also included.
pRoducts in action
Recently Released loW-teMpeRatuRe fReezeRs
Uses HFC CFC-free refrigerants Cascade system uses two air-cooled compressors Features a temperature range of -50C to -86C Features two access ports: one on back for CO2 backup; one on bottom for recording NuAire www.nuaire.com
Glacier 9668
Revco PLUS Series
Available in 17, 21 and 25 cubic foot capacities Feature up to 20% more heat removal capacity compared to ordinary freezers Introduce up to 15% less heat into the lab, to minimize air conditioning costs Feature an automatic voltage buck/boost system
Contacts: Wade Reichart wreichart@helmerinc.com Donna Holpuch dholpuch@helmerinc.com www.helmerinc.com
Platinum Series
The i.C3 is modular, and options can be added at any time. Options currently available include i.D Integrated Electronic Access Control. Future options will be available for release in 2011.
Access Control Screen may be used as the alternative home screen. Up to 100 User profiles may be programmed directly from the i.C3 Access Control Setup Screen. i.C 3 is easy to use and all functionality is easily accessed. As with our previous i.Center, all i.C3 settings are password protected for your security. The Icon Transfer screen allows users to customize Home Screen icons. In addition, Blood Bank Refrigerators will now feature 7 drawers in single door models and 14 drawers in double door models. Pharmacy Refrigerators will feature 6 roll out baskets and 1 shelf in single door models and 12 roll out baskets plus 2 shelves in double door models. This will increase storage capacity by over 17% in blood bank refrigerators. Adjustable LED spot lighting will provide energy savings as well as provide users with the ability to direct lighting where it is most needed. A rechargeable battery will offer 72 hours of backup protection in the event of a power outage. Other updates include an ON/OFF power switch, hospital grade power cord, and optional leveling feet. Contact Helmer at helmerinc.com for more information on i.C3 and the next generation of i.Series Products. Why wait for the future, when the future is NOW.
Upright models feature inner doors with anti-shock panels in polystyrene on a polyurethane core Cooling system is based on a cascade cycle using two hermetic compressors Plus models feature BIOGUARD, a proprietary door locking system with a personal identification tag for each user Angelantoni www.angelantoni.it
UF 755G and UF 455G
Feature temperature set points from -20C to -86C Vacuum Insulation Panel (VIP) offers improved insulation at a lower wall thickness Features stainless steel Rollbond evaporator technology for uniform and stable temperatures Inner chamber temperature variation: 3C at -82C (set point, preset) Baker BioScience Solutions www.bakerbio.com
Set for release in early 2011, all i.Series Products from Helmer will offer many new feature! The NEW i.C3 leads the way for the Next Generation of i.Series Products. The interactive temperature graph provides access to 42 days of temperature data and may be viewed in 1 or 7 day increments. Users may scroll to any day to view a graphical representation of the temperature function. Automatic alarm tests are noted with icons for documentation and conformance. Alarm events are represented by icons that are touched to automatically transport to the event log. All temperature data can be downloaded via the download screen for documentation. The optional i.D3 Integrated Electronic Access Control module provides secure access without the use of external locks. The lock is integrated into the door and is accessed via the i.C3. The
Low-teMpeRatURe fReezeR ManUfactUReRS

Cincinnati Sub-Zero New Brunswick Scientific NuAire Sanyo Biomedical So-Low The Baker Company Thermo Fisher Scientific www.cszindustrial.com www.nbsc.com www.nuaire.com www.us.sanyo.com www.so-low.com www.bakerco.com www.thermo.com
Full-color, 7 touchscreen is door mounted. Undercounter model shown with standard Home Screen. Upright model in shown with optional Access Control Home Screen.
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Mills and gRindeRs

Retsch Air Jet Siever, AS 200 Jet, for the Separation of Fine Particles
Particle size analysis and distribution are important criteria for the quality control of bulk materials. In an ever-changing environment, the results of a quality check must be available quickly and reliably to allow for immediate adjustment of production parameters. Depending on the expected particle size and sample volume, different sieving methods and machines are suitable for analysis. The traditional methods of sieving bulk materials with vibratory,
pRoducts in action
BRinging out tHe Best in analytical saMples

Mills and grinders are used to prepare samples (of minerals, plants, food, drugs, pigments, and forensic materials, for example) through particle size reduction (comminution). Afterward, samples are analyzed for their components or to demonstrate specific properties. Milling/grinding methods may be classified as impact, crushing, cutting, and abrasion, operations that Glen Mills (Metuchen, NJ) director Stanley Goldberg compares to using a hammer, pliers, scissors/ knife, and nail file. Over the last decade, specified target particle sizes have become smaller and size ranges narrower, two factors enabled by more powerful milling/ grinding machines that are safer and easier to clean and more rapidly achieve desired particle sizes. For the pharmaceutical industry, which is a growing market for mills/grinders, experts believe that many drugs that fail in clinical trials could be improved by producing them as nanoparticles or within very narrow size ranges. Similarly, existing solid drugs might benefit from tailoring their particle size. Lab-scale mills can rapidly produce small sample batches of a drug during product R&D for subsequent testing. Every major pharmaceutical company in the U.S. uses a planetary mill for that purpose, says Kyle James, sales manager at Retsch (Newtown, PA).
Labs are taking in more and more samples and need to process them in a shorter time frame.
Working in nanoscale provides greater bang for the buck, says Goldberg. Whether its greater coverage area for pigments, taste enhancement for foods, or improved efficacy for drugs, the control of size is important, and smaller is often better. Tim Osborn-Jones of Spex SamplePrep (Metuchen, NJ) says throughput is the leading issue factoring into purchase decisions. Labs are taking in more and more samples and need to process them in a shorter time frame. They have the option of doing one sample at a time manually or employing a miller or grinder that handles
multiple samples. Two other related factors are reproducibility and yield. Analytical labs must be assured that grinding a particular sample under specified conditions will always lead to the same result and provide the same quantity of the target material. Osborn-Jones says price is a relatively minor factor since the purchase price is paid back relatively quickly, particularly for grinders with automation features. Freezer mills (also called cryogenic mills or cryomills) have been around for about forty years. Cryogenic mills pulverize nonbrittle materials by first freezing and then milling them in specialized sample containers. Although not appropriate for every sample, freezer mills work wonders with samples that typically do not grind wellplastics, rubber, biological articles, and heat-sensitive materials, for example. When we used a conventional laboratory blender, our product was coarser and took twice as long to grind. Incorporating the freezer mill into our protocols has increased throughput and efficiency, says Sharhara Anderson at RiceTec (Alvin, TX), which researches rice plants and seed.
74 Walker Ln, Newtown, PA 18940 Phone: 866-473-8724 Fax: 267-757-0358 info@retsch-us.com www.retsch-us.com/as200jet
screen. This not only can result in erroneous data, but also leads to premature wear of the sieve. The Open Mesh Function of the AS 200 Jet has proven to be a key feature in eliminating near-mesh particles and prolonging the life of the sieve. This function moves the nozzle according to the principle two steps forward, one step back; the nozzle first moves forward by 20 then backwards by 10, instead of rotating uniformly (Fig. 3). With this technology, near-mesh particles are blown effectively from the mesh so no material lies on the sieve surface, obstructing the air jet.
horizontal or tap sieve shakers are limited to particles above 40 m. When using vibratory shakers for wet sieving, it is possible to sieve down to 20 microns. Air jet sieving (Fig. 1), however, allows for the dry particle separation and sieving of materials from 10 20 m.
the single sieve is placed on the unit and covered with a lid. A powerful industrial vacuum cleaner generates a strong jet of air which disperses the particles on the sieve, through the slotted nozzle rotating below the sieve mesh. Thus, the particles are dispersed with each rotation and are distributed over the complete sieve surface. Two unique effects occur: The jet of air causes a continuous new orientation of particles on the sieve surface. Particles with sizes smaller than the sieve apertures are pulled through by the vacuum cleaner (A cyclone attachment can be added to collect the smaller-than-mesh sample). When using 25 mm (1 inch) tall sieves, the inflowing air causes the particles to impact the lid, which helps to deagglomerate the sample. The speed of the nozzle and force of the air are both adjustable, enabling the user to customer the parameters according to their specific application. Sensitive samples (i.e. pharmaceuticals) can be sieved at slower speeds, while particles which tend to agglomerate (i.e. plastics, paints) benefit from aggressive air flow and fast nozzle rotation. Fine-meshed sieves are particularly susceptible to near-mesh particles which blind the sieve
Fig. 1: Air Jet Sieving Machine AS 200 jet by RETSCH
Fig. 2: Comparison of sieving times of commercial flour with vibratory sieve shaker and air jet sieve
The principle of air jet sieving

For dry sieving of samples with particle sizes below 20 microns, air jet sieving is the most efficient method. It can also be a faster alternative to vibratory sieving for particle sizes up to 200 microns (Fig. 2). In contrast to other methods, air jet sieving is usually carried out with only one sieve fraction. Together with the sample material,
Fig. 3: Open Mesh Function
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Mills and gRindeRs
ovens Basic laB
Recently Released MiLLS anD gRinDeRS
SM 300 Heavy-Duty Cutting Mill

Features a powerful, high-torque 3 kW (4.02 horsepower) motor Fold-back housing and removable, push-fit rotor allow for quick and easy cleaning Capable of processing power cords, batteries, circuit boards and LCD screens to a fineness of <2 mm Available in a heavy, metal-free version for contamination-free results Retsch www.retsch-us.com
Features automatic imbalance compensation and a grinding bowl lock system Able to grind larger sample quantities down into the nano range Includes a special emptying device with 2 sieves for grinding bowls EASY GTM ensures the set grinding parameters are not exceeded
PULVERISETTE 6 Planetary Ball Mill Premium Line
contRolling teMpeRatuRes to a fRaction of a degRee

Most basic lab uses employ oven temperatures from just above ambient to several hundred degrees Fahrenheit, although ovens used for materials processing reach temperatures in excess of 1000F. Kilns, specialty ovens used to process ceramics, may reach 2400F. Basic components common to all general-purpose lab ovens are an electrical heating coil, insulation, temperature measurement and/or recording, and a circulation mechanism that provides even temperature distribution. Advanced features include double doors, digital control, and temperature recording (useful for regulated industries requiring documentation). Cabinet ovens are used for batch processing, while conveyor designscommon with mediumto-industrial-sized process applicationsprovide continuous heating of many samples. Circulation ovens (the most common in labs) come in two types: gravity convection or mechanical (forced) draft. The former often suffer from temperature inhomogeneities and stagnation, which is why ASTM and AASHTO standards call for forced draft ovens. Larger labs primarily interested in glassware drying are better served by large ovens with customizable configurations than by hightech units with advanced controls. Materials testing or pharmaceutical development groups involved in drying or curing should focus on temperature stability/uniformity and perhaps automated recording and diagnostics. Users should modestly overbuy on temperature range to ensure that their applications will easily be covered. without fans to fan-based forcedair units. Fans distribute heat more rapidly on startup, and people are becoming less willing to wait for units to heat up, Ross observes. Fans provide more even heating by minimizing temperature
Fritsch www.fritsch.de/en
Micron-Master Jet Energy Mill

Designed to grind any type of crystalline or friable material, producing product in the size range of 0.25 to 15 microns Able to handle materials as diverse as talc and diamonds Available with IQ/OQ protocol documentation for Qualification Test Plans The Jet Pulverizer Company
Batch mill suitable for dry grinding of hard and brittle substances Double-walled grinding chamber can be cooled with water through two hose adapters Grinding chamber is removable for easy cleaning Two grinding chambers can be alternatively operated using one drive IKA www.ika.net
M 20 Universal Mill
People are becoming less willing to wait for units to heat up.
However, for a given heat rating, oversized ovens consume considerably more energy than compact designs, have a larger footprint, and may require specialized electrical hookups. Smart buyers whose oven volume and application needs vary often purchase several smaller ovens rather than one large one. Lab ovens range in size up to capacities of 25 cubic feet, but most applications employ units of 6 cubic feet and smaller. Other features to consider are general location, exhaust capabilities, mounting (floor or tabletop), fire/explosion protection, ambient or inert atmosphere, and controls/displays. Location is connected with unit size, ease of use, compatibility with other equipment, exhaust, and access to electric utilities. Uwe Ross, executive VP at Binder (Great River, NY) notes that in recent years, users preferences have shifted from gravity ovens variability within the oven, to the point where temperature distribution becomes a selling point. ASTM, for example, specifies an ovens temperature deviations by measuring at nine locations inside the oven, while the newer DIN (Deutsches Institut fr Normung) standard uses 27 points. Vendors supply temperature specifications, which vary from fractions of a degree in high-end ovens to several degrees. Users will tell you that an application works great on the middle shelf in the rear left cornerbut nowhere else, comments Ross.
www.jetpul.com
MiLL anD gRinDeR ManUfactUReRS

BioSpec Products Cianflone Scientific Glen Creston Glen Mills Fritsch Hosokawa Micron Powder Systems www.biospec.com www.cianflone.com www.glencreston.com www.glenmills.com www.fritsch.de/en www.hmicronpowder.com IKA Works Retsch Spectrum Chemicals Laboratory Products Spex SamplePrep Thomas Scientific UDY Corporation www.ika.net
SolutionS in milling & Sieving
E.A. Fischione Instruments www.fischione.com
www.retsch.com www.spectrumchemical.com www.spexcsp.com www.thomassci.com www.udyone.com
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pipettes Basic laB
Recently Released ovens Little SHOT III Medium Hybridization Oven

Provides rapid heat-up, excellent temperature stability, and efficient mixing Suited for all types of hybridization and incubation applications PID controller enables heat-up from ambient to 65C in 20 minutes with a stability of + 0.5C Forced air convection maintains excellent uniformity throughout the heating chamber Boekel Scientific www.boekelsci.com
OF-G Series Forced Convection Oven
Manual liquid Handling focuses on eRgonoMics

The high-throughput demands of modern laboratories have led to a rise in pipette-related repetitive stress injuries (RSIs). In a worstcase scenario, RSIs can lead to surgery and lost productivity costing tens of thousands of dollars. Improved ergonomics is therefore the most easily identifiable trend in pipettes. Materials used to manufacture pipettes have become lighter, and draw/dispense mechanisms easier to actuate. throughput needs, or multichannel pipettes for microtiter plate applications. Consider automating through a liquid-handling workstation. Electronic pipettes are becoming more popular because of the reduction in sample volumes and simultaneous increase in the number of samples processed. Electronic pipettes offer a reduction in the force necessary for dispensing volumes of water and weighing them on an analytical balance. Calibration usually takes place off-site at the calibrators facility, under conditions of strict environmental control and using standardized methods and materials. For example, Eppendorf employs a weighing vessel and a trap to reduce evaporation from small samples. The companys calibration room is maintained at 50 percent relative humidity and a constant temperature. Water used during testing is degassed and distilled; technicians test three different volumes for variable-volume pipettes; and all volumes are measured at least ten times. A typical calibration costs between $20 and $100 per pipette. Off-site calibration has a turnaround time of two days, while on-site calibration can be done the same day. Like most routine maintenance, calibration may be viewed as more of an inconvenience than a necessity. Some users are less concerned about the quality of calibration than about getting a sticker on their pipette that says its been calibrated, says Christian Petrilli, director of marketing at BrandTech Scientific (Essex, CT). But most recognize the value of a pipette that is calibrated properly. Certainly anyone in a regulated environment knows the importance of calibration.
Temperature range is 10C above room temperature to 250C Features an over-temperature limiter and door opening alarm Optional adjustable fan speed helps to prevent powder sample blowing Dual-wall door opens to 180 and features a double-rack door handle
Jeio Tech www.jeiotech.com/eng
SHEL LAB Model 1425 Vacuum Oven
FD Series Drying Ovens
Sizes range from 0.6 to 9 cu. ft.; include three shelves, seamless welds and strong bracing Features solid brass vacuum valves with Teflon seats to prevent leaks Features positive latch handles with spring-loaded glass for a good vacuum seal Temperature range is 10C above ambient to 240C
Available in four models, sizes ranging from 0.7 cu. ft to 8.6 cu. ft. Feature temperature range from 5C above ambient to 300C Include digital temperature setting with accuracy of one degree Feature homogeneous temperature distribution and rapid dynamic response
Improved ergonomics is the most easily identifiable trend in pipettes.

According to Andrea Dickstein, director of marketing at Eppendorf (Hauppauge, NY), preventing RSIs should be a top priority in high-throughput labs. She provides the following tips for selecting and using pipettes: operation, and multi-functionality in dispensing techniques. Calibration is perhaps the most important service-related activity for pipettes. It ensures that the pipette is delivering precise volumes after thousands of cycles. Regulated industries calibrate their instruments several times a year: all labs using pipettes should consider taking advantage of calibration services every six to twelve months. Some pipettes may be calibrated by the user, but most calibrations are carried out at the original manufacturers facility or through a third-party service provider. Some service companies perform calibration at the customers site. Calibration is conducted by
Sheldon Manufacturing www.shellab.com
BINDER www.binder-oven.us
oven ManUfactUReRS
BINDER Boekel Scientific Carbolite Cascade TEK Jeio Tech Labnet International LABREPCO Lucifer Furnaces MTI Corporation www.binder-world.us www.boekelsci.com www.carbolite.com www.cascadetek.com www.jeiotech.com www.labnetlink.com www.labrepco.com www.luciferfurnaces.com www.mtixtl.com Ontario Ovens SHEL LAB SP Industries Stovall Life Science Ted Pella Terra Universal Thermo Fisher Scientific Wisconsin Ovens Yamato Scientific www.ontarioovens.com www.shellab.com www.spindustries.com www.slscience.com www.tedpella.com www.terrauniversal.com www.thermoscientific.com www.wisoven.com www.yamato-usa.com
Make sure the pipette fits well in the hand. Determine that the volume is easily adjustable and that all buttons are easily accessible. Choose a pipette whose plunger mechanism requires low activation force. Make sure the operators hand can flex without removing the pipette. When using a pipette for more than two hours a day, choose an electronic pipette for high-
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pipettes
Xplore new ground with the Eppendorf Xplorer pipette
Features electronic piston stroke, low weight and intuitive user guidance to minimize user strain Features an innovative rocker: when tip is ejected, piston automatically returns to zero position Features a spring-loaded tip cone for maximum tightness Single-channel and multi-channel models are available Eppendorf North America www.eppendorfna.com
pRoducts in action
Recently Released pipettes
ErgoOne
Available in single-channel and multi-channel formats Tapered tip cones accept TipOne and other universal tips Plunger forces minimize stress and maximize control Light, balanced weight reduces fatigue USA Scientific www.usascientific.com
Xplorer Electronic
People who give 100% every day deserve the best tool and the best equipment. You work on demanding problems, and important decisions depend on the results of your work. Your standards are extremely high and your performance is professional. The new Eppendorf Xplorer electronic pipette was specially designed for high professional standards to provide optimal support for you in your work, with a new intuitive operating concept and design based on the proven Eppendorf PhysioCare Concept. These features set new standards for simplicity, precision and reproducibility, which mean no delays due to complicated programming or inflexible processes. Instead, you get precise adjustable parameters, reproducible results, fatigue-free work and full control over the pipetting processes. Reproducible results are essential for your laboratory work! Of course, dispensing results are affected by many factors; for example, by signs of fatigue or the aspiration and dispensing speed of the liquid. With the Eppendorf Xplorer, all relevant factors, e.g., volume, speed, mixing cycles, etc., can now be precisely and reproducibly set. Every day, consistently perfect. No matter where you are in the world, or which liquid youre using, the Eppendorf Xplorer can be individually adjusted to your environment and requirements. Program the Xplorer to an altitude or specific liquid density. Your unit can also be personally labeled to prevent an accidental exchange with another Xplorer. tional rocker make precise and flexible directional control of the piston possible. The Eppendorf Xplorer was developed and produced by renowned ergonomic experts according to the strict criteria of the PhysioCare Concept. The Eppendorf Xplorers perfect balance and hand rest guarantee a low-impact position and efficient relaxation periods. The handle and size of the control buttons feature a user-friendly design, regardless of hand size.
When tips are optimally seated
Features a 50% drop in tip insertion forces and line-of-sight enhancements to further minimize physical effort and save time when acquiring pipette tips Four 12-channel models are available for precision liquid dispensing from 0.5 L to 850 L Four 8-channel models are available for volumes from 0.5 L to 1,250 L Three user-defined volume settings can be stored for later recall with a single keystroke
Ovation Multichannel
HandyStep Electronic Repeating
Automatically recognizes the size of encoded proprietary syringe tips Features continuously adjustable volume selection from 1 L to 50 mL Compatible with standard syringe tips from variou MANUFACTURERS Features three operational modes: Pipette, Dispense, and Auto-Dispense, which eliminates up to 97% of repetitive motion from repeat pipetting
The Eppendorf Xplorers spring-loaded tip cone provides maximum tightness with inimal attachment force, noticeably reducing the energy required for ejection without compromising tightness. Because the tip always rests in the same position, the reproducibility of your results is heightened.
Improved view
VistaLab Technologies www.vistalab.com
BrandTech Scientific www.brandtech.com
pipette ManUfactUReRS
Biohit Boekel Scientific BrandTech Drummond Scientific Eppendorf Gelcompany Gilson Globe Scientific Hamilton Hirschmann www.biohit.com www.boekelsci.com www.brandtech.com www.drummondsci.com www.eppendorf.com www.gelcompany.com www.gilson.com www.globescientific.com www.hamiltoncompany.com www.hirschmann-inc.com Jencons Scientific Labnet International Nichiryo America Oxford Instruments www.jenconsusa.com www.labnetlink.com www.nichiryo.com www.oxford-instruments.com
Everything at a glance with one click
You can easily select all functions with the practical selection dial. The clearly arranged color display of all adjustable parameters means no more getting lost in the submenus! An optional help function can be installed in your language of choice.
All information on the Xplorer display is clearly readable, regardless of your hand position or work position. The optimally angled pipette head and high-resolution display with individual brightness setting guarantee a comfortable work environment.
Improved balance of energy
Simple logic
Rainin Instrument Company www.rainin.com Rice Lake Weighing Systems www.ricelake.com USA Scientific VistaLab Technologies www.usascientific.com/ergoone www.vistalab.com
To make pipette operation even more logical, we have developed a unique multi-function rocker for the Eppendorf Xplorer. The switch enables ultra precise control of liquid aspiration and dispensing. It follows the Up is up and down is down principle: Press the rocker up to aspirate liquids and down to dispense liquids. When the tip is ejected, the piston automatically returns to zero position.!
The more energy you need to expend, the quicker your energy reserves are depleted. But because your performance is the most valuable resource, we have significantly reduced the weight and required operating force of the Eppendorf Xplorer.
Wheaton Science Products www.wheatonsci.com
Everything under control
For slow pipetting, loading gels or dispensing supernatant, a feel for your task is requiredand complete control of the piston movement! With the Xplorer, the Manual pipetting function and operaProduct Resource Guide 2010 Lab Manager 93
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RefRigeRatoRs/fReezeRs
Laboratory Refrigerators and Freezers.
Nor-Lake Scientific Select laboratory Refrigerators and Freezers are designed for heavy-duty long term cold storage for a wide range of demanding laboratory, scientific, pharmacy and life science applications. Product selection includes fullsize reach-in +4C Solid and Glass Door Refrigerators, Sliding Glass Door Refrigerators, Pass-thru Refrigerators and -25C Freezers. Select Refrigerator and Freezer models are available with one, two or three doors. Pass-thru models are available in two, four and six door configurations with solid or glass door combinations. Chamber capacities include internal volumes of 24ft3, 33ft3, 53ft2 and 80ft3.
pRoducts in action
a teMpeRatuRe foR eveRy taste

Lab refrigerators and freezers are similar in construction to household units, and come in a variety of temperature ranges, shapes and sizes. Freezer and refrigeration options fall into four general temperature categories: +4C refrigerators for chromatography supplies, blood storage, and pharmaceuticals; -20C (and below) freezers for enzymes and biochemicals; -30C to -40C for biological samples, and -80C freezers for long-term storage and stability. Units range in size from under-counter systems as small as 3.6 cu. ft., to standalone chests as large as 70 cu. ft. After considering temperature capabilities, capacity and footprint, choosing among lab refrigerators and freezers often reduces to secondary or subjective criteria. Eye-level controls, mechanisms to prevent door freezing and/or vacuum pressure build-up, space-saving insulation, automated data recording, alarms, digital temperature control, rapid temperature recovery after door openings, temperature uniformity throughout the box, and condition monitoring are differentiators. However, purchase decisions often come down to brand name recognition, perceived reliability, price and availability. Tamper-proof temperature logging and recording are desirable features for regulated industries and those called on to testify in court. Thermo Fisher Scientific (Marietta, OH) plans to offer a wireless recording/logging option in 2010, but companies have been slow to adopt such equipment due to difficulties in changing established SOPs. The traditional chart recorder is still the industry standard for temperature monitoring. However, most industries are moving toward electronic temperature monitoring. One notable development in refrigeration has been the emergence of cold storage to support vaccine work, particularly for H1N1 influenza vaccine storage. H1N1 vaccine is stored within a narrow temperature range (35F to 46F), and health agencies mandate twice-daily temperature measurements.
chests for labs. Common upgrades include alarms, controllers, door locks and special shelving. Energy efficiency has become a key driver in refrigerator/freezer purchase decisions. Many vendors have been working with the U.S. Environmental Protection Agency to establish industry-wide Energy Star standards for lab refrigerators and freezers, which surprisingly do not yet exist. Beginning in late 2008, vendors began submitting energy efficiency and performance data to EPA, from which the agency will eventually issue guidelines for the coveted Energy Star designation. Energy consumption for always-on appliances is a serious concern for large organizations like pharmaceutical companies and universities.
Nor-Lake Scientific 727 Second Street Hudson, WI 54016 800-477-5253 www.norlakescientific.com
Select models feature advanced digital LCD 4x20 character display, microprocessor control system with key pad. Includes audible and visual high/low alarms, 2 product sensors with glycerin bottles, 2 to 10 volt DC output, remote alarm contacts, door ajar alarm, password protection of set points and factory settings, real time clock, operation diagnostic monitoring of defrost, compressor and fan, low and high alarm test, event logging and sensor failure alarm. Cabinet construction features include white painted exterior front, sides and back, with galvanized steel on the top and bottom. The interior is white and there are three epoxy-coated shelves per door, which can be adjusted in 1 increments. Standard features include interior lights (switch activated), easy roll low profile casters, magnetic door gaskets, key door locks, and 1diameter lead sensor port.
Cabinets are formed-in-place with high-density CFC-free polyurethane foam insulation. Doors have heavy-duty pivot hinges and pull handles. Select Refrigerators and Freezers feature top-mounted refrigeration, air-cooled condensing unit and automatic condensate evaporation. The combined features of the Select control refrigeration system and cabinet construction produce a precise, uniform controlled temperature environment and energy efficient operation for long lasting reliable and durable performance. Available options (depending on model) include: extra shelves, stainless steel drawers, sliding basket drawers, temperature chart recorder and chart paper, stainless steel interior and/or exterior, reverse hinge doors, 4-20ma output, RS485, seismic mounting, Secure Guard lock system, internal electrical outlet, access port 2 sleeve with cover and export crating.
purchase decisions often come down to brand name recognition, perceived reliability, price and availability.
Many labs use household kitchen refrigerators to store very low-risk laboratory materials, but these units are designed for low-traffic use and lack the precise temperature control and refrigeration capabilities of lab-designed units. Several vendors have nevertheless made a business of refurbishing and retrofitting home-appliance cooling Due to their heavy usage, lab refrigerators and freezers will never be as energy efficient as units purchased for the home, said Gordon Shields, director for cold storage at Thermo Fisher Scientific. The key is to reduce overall energy usage while maintaining performance.
Nor-Lake Scientific pass-thru refrigerators have a front door and a rear door, so they can be accessed from two sides. They are available with one pair, two pairs or three pairs of doors in a variety of materials: with glass front door and solid back door, with all glass doors or with all solid doors. Available with sliding baskets as shown.
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Marvel Scientific: Refrigeration for Health, Science and Industry
Serious about Standards
In laboratories worldwide, household refrigerators are pressed into duties they were not designed to fulfill. When these units operate inconsistently or fail, consequences can range from inconvenient to costly to dangerous. At Marvel Scientific, we believe its time to get serious about standards. Marvel Scientific refrigeration products are engineered and constructed to meet or exceed the stringent standards required by health, science and industry. Each general purpose, flammable material storage and explosion proof product has been designed specifically for the lab with a Commercial UL Listing for your peace of mind. Let Marvel Scientific help you professionally protect your research, your specimens and your lab.
pRoducts in action
Recently Released RefRigeRatoRs/fReezeRs 6CADM Series Jewett Collection
Features MicroSentry Scientific control for temperature accuracy and monitoring Includes a powerful compressor for faster pull-down times and quick temperature recovery Features positive-close hinges to reduce door ajar occurrences Front-vented for true integration into laboratory casework
Designed to store high value, critical samples, reagents, vaccines, blood and plasma 24 models range in size from 4.9 cu. ft. under-counter styles to 51.1 cu. ft. uprights Include microprocessor control, giving the user precise temperature regulation and set-point security Feature powerful industrial-grade compressors and positive, forced-air circulation
P.O. Box 400 Greenville, MI 48838 Tel: 1-800-962-2521 | Fax: 1-616-754-0970 E-mail: lferguson@agamarvel.com www.marvelscientific.com
Marvel Scientific www.marvelscientific.com
Thermo Fisher Scientific www.thermoscientific.com/cold
Superior Temperature Control, Accuracy and Monitoring

Marvel Scientific refrigerators have always been the most dependable and reliable, maintaining stable temperatures to protect valuable research and costly materials. Now, that reliability is measurable and recordable. New general purpose lab refrigerators feature the exclusive MicroSentryTM Scientific refrigeration control, with the ability to control, confirm and capture temperature performance, giving you the ultimate confidence in your refrigeration. The MicroSentryTM Scientific control is an interiormounted digital electronic control that allows you to set temperature, high/low alarm values and alarm delay period. Temperature is confirmed via the large LED display and audible/visual alarms notify if there is a high/low temperature event or if the door is left open. A remote alarm interface allows communication of any alarm event, including power failure, to a remote or central monitoring system. There is a history record of high/low temperatures for monitoring programs, and notification of periodic maintenance and diagnostic codes. Lab refrigerators with the MicroSentryTM Scientific control are available in 15 and 24 undercounter models, with either a solid or glass door. All models are front vented so they can be built into casework with zero clearance.
Available in capacities of 19 cu. ft. up to 72 cu. ft. All standard shelves are epoxy coated wire (SS shelves available) Sliding doors or swing-out doors are available Includes one year parts and labor warranty with additional four year compressor warranty MidSci www.midsci.com
ArcticTemp
Features front ventilation and a bacteria-resistant powder coating Features a field-reversible Sure-Seal door design and interior volume of 5 cu. ft. Customizable with interior lights, swivel casters, stainless steel interior/exterior and glass door Operating temperature range is 2C to 10C, factory set at 4C Helmer www.helmerinc.com
Scientific Series
access even from a seated position. Two super glide, full-extension drawers store all types of containers and provide superior visibility and accessno more reaching to the back of a crowded refrigerator shelf. The two-drawer lab refrigerator features interior lighting, automatic defrost, hidden touchpad controls with LED digital display, extra deep drawers and heavy duty drawer slides for full loads. Full-wrap drawer fronts come in white or stainless steel with matching handles.
ADA Height Lab Refrigerator

Designed to comply with the height standards set by the Americans with Disabilities Act (ADA), Marvel Scientifics 6CADM general purpose laboratory refrigerator addresses the differing physical needs of individuals. Title III of the ADA requires that all new construction of, or modification to, public and commercial facilities be accessible to individuals with disabilities. Because the 5.4 cu. ft. laboratory refrigerator is built for lower countertops and users of all abilities, its design includes numerous unique features. Two slide-out shelves feature safety stops to prevent shelves from being disengaged from the refrigerator. The top shelf includes an ingenious flip-up section to vertically store tall items on the shelf below. The model 6CADM now features the MicroSentryTM Scientific controller, ultra efficient compressor, lifetime fan motor, and Commercial UL and C-UL listing to ensure optimum performance and unwavering reliability. An optional door lock and access port are available when product security or alarm probes installation require them.
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RefRigeRatoR/fReezeR ManUfactUReRS
BioCold Darwin Chambers Helmer Kelvinator LABNICS Equipment Marvel Scientific New Brunswick Scientific Nor-Lake Scientific NuAire Sanyo So-Low The Baker Company Thermo Fisher Scientific www.biocold.com www.darwinchambers.com www.helmerinc.com www.kelvinator.us www.labnics.com www.marvelscientific.com www.nbsc.com www.norlake.com www.nuaire.com www.sanyobiomedical.com www.so-low.com www.bakerco.com www.thermo.com/cold
Innovative Two-Drawer Lab Refrigerator

Marvel Scientifics 6CRDE is the first Two-Drawer Undercounter Lab Refrigerator with a Commercial UL Listing. The unique configuration allows full
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specialty gases
Recently Released specialty gases Specialty Gas Regulators NitroVap-1LV and NitroVap-2LV
Produce more than 320 LPM of pure evaporation grade nitrogen Require less than 1 square foot of bench space and can supply more than 100 evaporator nozzle positions Use a combination of filtration and membrane separation technologies High-efficiency coalescing filters remove all contaminants down to 0.01 micron Parker Balston www.labgasgenerators.com
a guaRantee of puRity
Defining the term specialty gas seems simple at first. But after considering the numerous ways specialty gases might differ from their commodity counterparts, it becomes tempting to apply the aphorism I cant define it, but I know it when I see it. Bob Jefferys, marketing communications director for Air Liquide America Specialty Gases (Plumsteadville, PA), applies what is arguably the number one differentiator. Specialty gases are very high-purity products consisting of single gases, up to mixtures of fifty or more individual components, says Jefferys. And they tend to be a lot more expensive than run-of-the-mill industrial gases. For example, high-purity helium is employed as the mobile phase for gas chromatography. Its not the same product youd use to inflate helium balloons at a birthday party, Jefferys says. The definition of specialty gas comes up at every training session I conduct, says Todd Morris, director of laboratory markets at Airgas (Radnor, PA). The basic definition I use is any gas thats tested for purity after the cylinder is filled. That can include pure gases like nitrogen, oxygen, and argon, or custom gas blends. All major ga MANUFACTURERS provide specialty pure gases in several grades, ranging from high purity (99.998% min. purity) to ultra-high purity (99.999% min. purity) to research grade (99.9999% min. purity). Each grade is tested for a defined set of impurities, such as levels of oxygen, moisture, total hydrocarbons, carbon monoxide, nitrogen, and others.
for customers to check their gas inventories. Two main factors enter into specialty gas purchase decisions: price and quality. For gases, quality means some desirable combination of purity and mixture accuracy. For each end
Made from solid bar stock with a needle valve built in the regulator body to reduce internal volume Feature stainless steel diaphragms for consistent, high-purity results Check-valve cylinder connection option prevents contaminants from entering gas stream during cylinder change out Airgas www.airgas.com
For gases, quality means some desirable combination of purity and mixture accuracy.
Like commodity gases, specialties are distributed under an unusual economic model: Customers purchase the gas and rent the container (usually a steel or aluminum high-pressure cylinder fitted with a heavy-duty valve). Depending on the size of the container and the type of gas, the costs can run from $100 to as much as $3,000. Rental, or demurrage, typically amounts to less than a dollar per day per cylinder. While not expensive for a single tank, costs can add up quickly for large companies with hundreds of cylinders. Because gases are often deployed at the laboratory or individual level, large organizations have difficulty keeping track of which cylinders are empty or are no longer in service. Best-in-class specialty gas companies send out periodic email messages or other reminders use, the grade required is entirely customer-driven. The first thing we ask when a customer specifies a pure gas is which impurities will adversely affect their application, notes Morris. Once that is understood, it is relatively straightforward to set the customer up with the appropriate gas. The specialty gas business has become more competitive during the past decade, with many smaller businesses being acquired by large manufacturers. At the same time, end users have become more demanding in terms of specialty gas purity and composition. Keeping up with the marketplace involves staying abreast of gas analysis and blending techniques. A major supplier should maintain a high degree of technical expertise, Jefferys says, in order to retain its customers.
Perma-Cyl Gas Vessel
Allows liquid to be held for long periods of time without venting, limiting product loss Auto shut-off feature allows remote filling with wall box and piping Constructed of stainless steel Features a Cyl Tel gauge on 450-, 1,000-, and 1,500-liter models Middlesex Gases & Technologies www.middlesexgases.com
ALPHAGAZ Series
Available in three grades and two purity ranges ALPHAGAZ 2 exhibits the absolute lowest impurity levels available in the industry ALPHAGAZ 1 exceeds purity recommendation of most laboratory instrument manufacturers, making it suitable for general lab use Air Liquide www.alspecialtygases.com
SpeciaLty gaS ManUfactUReRS

Airgas Air Liquide Air Products and Chemicals CONCOA/Controls Corporation of America Matheson Tri-Gas Norlab Parker Balston Praxair www.airgas.com www.airliquide.com www.airproducts.com www.concoa.com www.matheson-trigas.com www.norlab-gas.com www.labgasgenerators.com www.praxair.com
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toc analyzeRs
Recently Released toc analyzeRs 9210e Fusion
suRRogate MeasuReMent foR cHeMical, BacteRial contaMination

Total organic carbon (TOC) analysis measures the carbon content of dissolved and particulate organic materials in water. Its nonspecific, meaning it tells how much organic carbon is present without identifying the contaminant. The carbon measured in TOC analysis may arise from any combination of living or dead organisms and chemical contamination. Water utilities use TOC to monitor by-products of chlorination or ozonation. TOC often serves as a surrogate for more difficult measurements, for example, contamination from petrochemicals, solvents, pharmaceuticals, chlorinated industrial chemicals, and pesticides. It can also act as a screen for additional analysis. For example, pharmaceutical manufacturers might use liquid chromatography-mass spectrometry to analyze water samples containing unacceptable TOC values. The two main approaches to TOC measurement involve either initial removal of inorganic carbon (mostly carbonate) followed by TOC measurement, or the subtraction of inorganic carbon from total carbon present. The four steps in TOC measurement are acidification to remove inorganic carbon, purging to release volatile organics (which are measured separately), oxidation of the remaining carbonaceous material, and detection. The latter two operations form the heart of TOC analysis. Several types of oxidation may be used: high- or low-temperature combustion, catalytic oxidation, photo-oxidation, thermochemical oxidation, or electrolytic oxidation. Detection limits for TOC depend on the measurement technique used and the type of analyzer. Hightemperature (up to 950C) oxidation produces a sensitivity of 0.1 mg/L of carbon, while low-temperature methods (below 100C) are five times as sensitive, to about 0.02 mg/L. Response times for TOC analyzers vary widely, but instruments generally take five to fifteen minutes to report stable readings. Online TOC analyzers are capable of continuous, unattended operation, but regular calibration, inspection, and maintenance by skilled technicians is required for reliable operation. TOC analyzers are reasonably priced as lab instruments go. A basic unit costs approximately $20,000. Autosamplers will add to the cost, as will the addition of detectors for nitrogen or isotopic carbon. Over the past decade, the popularity of TOC analysis has been driven by regulations, notes Steve Poirier, VP of business development at GE Power and Water (Boulder, CO). Every pharmaceutical company that ships drugs into the U.S. and Europe is required to measure TOCs to certain specifications. In the drug industry, high-purity water is used both for cleaning and in sterile drug products.
Ensures processed and purified water is safe for human consumption Maintains excellent long-term calibration stability, providing accurate and dependable data with minimal maintenance Created using reagentless electrochemical oxidation technology developed for use on the International Space Station Efficiently oxidizes organic compounds using hydroxyl radicals, peroxides and ozone OI Analytical www.oico.com
Employs UV Persulfate oxidation for increased carbon liberation from challenging matrices Uses static pressure concentration (SPC) technology, for low-end sensitivity from a NDIR detector Allows unattended auto-calibration monitoring and automated self-diagnostic tools Teledyne Tekmar www.teledynetekmar.com
Sievers InnovOx On-Line
TOC analyzers are reasonably priced as lab instruments go.

The second regulatory front is the environment. According to Poirier, every municipality of greater than 10,000 population is required to control TOC to specified levels in drinking water. Additionally, some companies have demonstrated equivalency between TOC measurements and other tests and use TOC as the primary regulatory assay for releasing wastewater, Poirier adds. The advantages are that TOC analysis is straightforward and does not require the specialized skill set of chromatography. But in the end, most purchase decisions are based on productivity, and that comes down to analysis time or throughput, Poirier says.
Supercritical Water Oxidization (SCWO) technique offers enhanced reliability, ease of use and low maintenance Handles difficult samples such as brine, humic acid and cellulose Features a dynamic linear working range of 0.5 to 50,000 ppm
astroTOC UV Turbo
Features a response time of less than 5 minutes with 100% oxidation Multi-point calibration compensates for background TOC in make-up water Available in ranges of 0-2000 up to 0-50,000 g/l Housed in a spacious, robust, dual-compartment enclosure
GE Healthcare www.geinstruments.com Hach Company www.hach.com
toc anaLyzeR ManUfactUReRS

Analytik Jena EST Analytical GE Analytical Instruments Hach Company OI Analytical Parker Balston Shimadzu Scientific Instruments Skalar Analytical Teledyne Analytical Instruments Thermo Fisher Scientific TOC Systems UIC www.analytik-jena.com www.estanalytical.com www.geinstruments.com www.hach.com www.oico.com www.labgasgenerators.com www.ssi.shimadzu.com www.skalar.com www.teledyne-ai.com www.thermo.com www.tocsystemsinc.com www.uicinc.com
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vacuuM puMps
pRoducts in action
oil-fRee alteRnatives offeR nuMeRous advantages Besides Being Kind to tHe enviRonMent
Costs associated with generating a vacuum, such as process costs, user costs and cost to the environment, have never really been considered seriously. Although technology has advanced to provide smaller, cleaner and quieter options, not many people are taking advantage of it. Vacuum pumps last a long time and many people go through their careers without actually buying one, says Peter Coffey, vice president of sales and marketing at Vacuubrand. Hence, people tend to replace their vacuum pumps with what they have used before and not take the time to find out about the alternatives. Vacuum pumps used in laboratories can be classified into two categoriesrotary vane pumps, sometimes referred to as oil-lubricated pumps, and dry (oil-free) pumps. They operate in different ways to create vacuum and aspirate fluids. Centrifugal pumps use centrifugal force to push the fluid through an outlet; metering pumps, such as diaphragm, peristaltic, piston and syringe pumps, pull fluid into a chamber and then push it through the outlet valve; while positive displacement pumps use bellows, piston, rotary lobe and rotary vane to push fluid through a cavity, leaving a vacuum that pulls in more fluid. Oil-lubricated pumps have been around for many decades, while oil-free diaphragm pumps are a says Coffey. If you use your vacuum pump 20 hours a week, 50 weeks a year, thats 10 years before the first scheduled service! A misunderstanding regarding oil-lubricated pumps is that people assume more vacuum is always better. Oil-free diaphragm pumps can provide vacuum levels from atmosphere to below 0.5 mbar/Torr, whereas oillubricated pumps offer high capacities and higher vacuum levels up to 10-3 mbar/Torr. What is more important is matching the vacuum to the application, and almost any application can now be performed using an oil-free pump, with the exception of freeze-drying, says Coffey. For certain applications he also recommends considering buying a pump with controls that provide a good balance of speed and control. Even manual controls are better than none, but electronic controls offer huge productivity advantages, he says. In many applications, the type of vacuum control used will determine how much scientist time is needed for oversight and how fast the application proceeds.
KNF NEUBERGERS VACUUM PUMPS & SYSTEMS KEEP ON GOING FOR CLEAN, RELIABLE, OIL-FREE VACUUM IN YOUR LAB
At KNF Lab, we know youd rather work on your research than on your equipment. No matter the amount, frequency, or kind of solvents you use, weve designed our chemically-resistant diaphragm air, gas and liquid pumps to keep on going, so you can too. Used for a wide variety of applications including rotary evaporation, filtration and liquid metering/dosing, researchers find these versatile pumps to be extremely quiet and sturdy even when continuously running corrosive solvents. LABOPORT vacuum pumps feature premium chemical resistance and oilfree design, and are perfect all-purpose pumps for your laboratory researcher. We think youll like them because of their light weight and small footprint physically and environmentally. See more online at www.knflab.com.
Two Black Forest Road Trenton, New Jersey 609-890-8600 www.knf.com
KNF Neubergers SC920 vacuum pump system (right) includes a wireless

remote control for easy monitoring and control of vacuum system functions, even behind a closed hood sash from across the lab. Whether adjusting the system pressure or opening/closing the coolant valve, researchers can instantly make a change using the touch screen and control knob on the hand-held remote control. For instance, say you want to have precise control over vessel pressure our system display allows you to monitor and control it to defined values, making adjustments while the system is safely behind a closed sash. Thanks to this remote control capability, it is no longer necessary for the vacuum system to occupy valuable bench space. Instead, position the SC920 under your lab bench or in a fume hood.
Pressure Control: The vacuum system regulates pumping speed to achieve and maintain the desired vacuum setpoint. Automatic Mode: The vacuum controller automatically finds the vapor pressure of the sample and regulates pumping speed to maximize evaporation, then monitors for changes in the pressure curve and optimizes pumping speed instantly. Individual Pressure Function: The system regulates the vacuum according to a user-defined pressure curve. The setpoint pressure and the opening/closing of the coolant valve are easily entered for a set range of times. A repeat function allows successive repetitions of the programmed pressure curve. With our wireless technology youll conserve precious lab resources by keeping the fume hood sash closed during processing. This will reduce building heating and air conditioning costs. By accessing all system functions from outside a closed hood, harmful vapors are less likely to enter the lab, and give you some protection if things go wrong, reducing everyones exposure to solvent vapors, glass breakage and chemical spills. Like the remote control, the included Windows based software makes it possible to display pressure curves as charts, which can then be saved as permanent logs and exported to spreadsheet or text files.
people tend to replace their vacuum pumps with what they have used before and not take the time to find out about the alternatives.
more recent addition. Although oil-free pumps tend to be more expensive than oil pumps, there are a lot of advantages to their use as well as significant savings. First of all, no oil is used, and therefore there is no cause for oil contamination and no necessity for oil change or disposal. Oil-free pumps can be built to be corrosion resistant and hence do not require regular maintenance. Service intervals on better oil-free pumps exceed 10,000 operating hours,
How the SC920 system works

At the start of a process, the pump operates at low speed to prevent any overreaction to small chamber volumes or low boiling solvents. A pressure sensor measures the actual pressure in the system several times per second, and compares it with the programmed setpoints. The microprocessor then monitors any changes in pressure curve and adjusts the pumps performance instantly. This ensures fast process times with exceptional accuracy and repeatability. The SC920 vacuum pump system offers four different operating modes: Evacuation: The vacuum system pumps down at full speed.
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VACUUBRAND PC3001 VARIO
The VACUUBRAND PC3001 VARIO vacuum pumping system provides self-regulated control of evaporative applications like rotary evaporators and concentrators without test runs or programming. The unit monitors vapor flow from the application and adjusts its own pumping speed to continuously optimize evaporative conditions. It detects multiple boiling points in complex mixtures and generates the pressure curve that accelerates the process while minimizing bumping and foaming caused by over-pumping. The result is an evaporation that is often completed in 30 percent less time than with conventional two-point electronic control, while protecting samples and minimizing need for operator intervention. A computer link is available for process documentation. VARIO vacuum pumping systems are also available for evaporations up to pilot scale.
pRoducts in action
Recently Released vacuuM puMps VACUUBRAND ME1 and ME1C HiPace Series
Deliver pumping speeds of 14 lpm and ultimate vacuum of 100 mbar Feature robust PTFE diaphragms and valves for optimal chemical resistance Feature a well-proven and long diaphragm life of 10,000 to 15,000 operating hours ME1C features a full fluoropolymer flowpath, for a complete chemistry-design pump
Feature a range of pumping speeds from 10 to 800 liters per second Integrated drive electronics reduce the need to costly cabling Installation is possible in any orientation Features a sealing gas connection to safeguard bearings
Tel 860-767-5341 www.vacuubrand.com
BrandTech www.brandtech.com
Pfeiffer Vacuum www.pfeiffer-vacuum.com
Inverter electronic allow user to tailor pumping speed to each application Features a high pumping speed over a wide range, from atmosphere to 1 mbar (0.75 Torr) Features a base pressure below 5x10-2 mbar (3.75x10-2 Torr) T-Plus software enables control of pump parameters from a PC
MS40+ Rotary Vane
Varian www.varianinc.com
Speed and stroke controlled operation for a precise hysteresis-free vacuum Features a sound insulation system, full housing enclosure and weight balance for quiet operation Includes an integrated gas ballast for a constantly high suction volumes Three-stage vacuum creation process delivers 3.1 m3/h at a low final pressure of less than 2 mbar Buchi www.buchi.com
V-710
What is the most valuable resource in the lab? Reagents? Equipment time? Sample material? No. The most valuable resource in the lab is the time and intellect of the researcher. Why use that precious resource to babysit evaporative applications? No one would consider manually maintaining temperature of critical processes introducing and removing a heat source manually to maintain a constant temperature. Why waste precious researcher time on manual control of vacuum applications? VARIO vacuum control technology relies on innovative electronics to detect vapor conditions in evaporative applications and automatically and continuously adjusts vacuum to optimize conditionswithout programmingto accelerate processes while protecting samples. Consider the rotary evaporation of a mixture of solvents. Vacuum provided by an uncontrolled pump often leads to over-pumping, exceeding vapor pressures needed for evaporation and leading to boil-over and loss of sample. Manual control of vacuum conditions requires continuous oversight, adjusting vacuum to accommodate the continuously changing vapor pressure of the sample mixture as the more volatile solvents evaporate. Its manageable if inefficientwith a major commitment of concentrated attention, but arent there better ways to use your time and education? Electronic two-point control is another option. You can pick optimum conditions for one solvent
out of a manual or solvent library, and set a target vacuum pressure, expecting the system to maintain conditions the way that a thermostat controls ambient temperature or the heat on a hotplate. Unfortunately, the proper set-point changes as the more volatile solvent evaporates, so manual controland systems that find only the first boiling pointfall short. Soon, vacuum conditions will be less than optimal, and the process will slow to a crawl. You could program set-points and a ramp, but only by running timeconsuming trials and programming the controller to maintain vacuum conditions at an approximation of optimum as it changes over time. The better option is with VARIO control by VACUUBRAND. The VARIO controller monitors vacuum conditions and continuously adjusts pumping speed to optimize vacuum conditions moment to moment. There is no over-pumping, as with uncontrolled vacuum; no time-wasting babysitting to control the evaporation manually. No test runs or programming of approximate conditions. Instead, you press Start, and rely on the VARIO controller to avoid overpumping that causes bumping and foaming and loss of sample, while achieving the highest evaporation rates that protect your samples. Should your application be destined for scaleup, VACUUBRANDs PC3002, PC3004 and PC3010 VARIO systems let you easily scale up evaporations to 20 liters or more with the same level of precision and ease.
vacUUM pUMp ManUfactUReRS

Alcatel BrandTech Buchi Cole Parmer Ebara Edwards Gast Manufacturing Ilmvac KNF Myers Vacuum www.adixen.com www.brandtech.com www.buchi.com www.coleparmer.com www.pumpsebara.com www.edwardsvacuum.com www.gastmfg.com www.ilmvac.com www.knf.com www.myers-vacuum.com Oerlikon Pfeiffer Vacuum Precision Scientific Shimadzu US Vacuum VACUUBRAND Vacuum Research Varian Welch www.oerlikon.com www.pfeiffer-vacuum.net www.precision-scientific.com www.ssi.shimadzu.com www.usvacuumpumps.com
technology for vacuum SyStemS
www.vacuubrand.com www.vacuumresearch.com www.varianinc.com www.welchvacuum.com
New Star Environmental www.newstarenvironmental.com
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pRoducts in action
MoRe tHan a convenience

Many labs today rely on glassware washers, as they ensure consistent cleaning of critical labware, free up technician time for more value-added work, and provide assurance and validation in regulated industries. Generally, the more critical the research, the more likely it will employ an automated washing process, observes Ken Austin, who manages Miele Professionals (Princeton, NJ) laboratory division. Efficient cleaning is a function of cycle time, wash temperature, mechanical action, and cleaning agents. The ideal combination is high water throughput at relatively gentle spray pressure, sufficiently high temperature, a spray pattern that reaches the entire wash load, and selection of cleaning agents suitable for the task. Because of the heavy demands placed on cleanliness and the unusual form figures of labware, unmodified household dishwashers are not suitable for laboratory use. For example, the pump in a high-end under-counter glassware washer is rated at a minimum of 100 gallons per minute, compared with 25 to 30 gallons per minute on a home washer. Cycling time for lab units is longer; holders and baskets are customized for lab equipment; and the final rinse uses heated, recirculated, deionized water instead
Still Handwashing?
the general aversion to washing glassware. But central washers often do not thoroughly clean items, and breakage is higher than when scientists wash their own. Researchers dont trust the wash room, and wind up cleaning their critical pieces anyway. Increasingly, lab managers and facility designers are specifying lab washers based on environmental and ergonomic factors. Purchasers are interested in how the machine coordinates with the building, the lab, and an organizations green strategies, says Mike Henley, general manager at Lancer (Winter Springs, FL). Utilization of water, energy, and cleaners and wastewater disposal are important factors that affect operating cost. Purchasers have moved beyond up-front cost and are beginning to take these other issues into account. Research space is expensive, and so are salary and upkeep of personnel. They dont come to work to wash glassware, Henley notes.
of tap water. Some lab-designed machines employ bottom-heating for drying, as do kitchen units, but high-end washers employ forced HEPA-filtered air. Many kitchen models lack a pump powerful enough to provide effective wash coverage; others with more robust pumps cause glassware to launch and break, Austin adds. Factors to consider include type of labware and residues being cleaned, throughput (pieces per day), and current wash methods. Labs operating in regulated industries, or those that come in contact with the legal system, should consider automated controls that verify and validate cleaning cycles, from initial wash through drying. The ultimate machines of this type are validated to pharmaceutical Good Manufacturing Practices. Austin says a trend he sees is the use of individual lab washers in facilities where a central wash room had been the norm. The interplay between centralized and individual lab washing involves
Get repeatable results with the G 7883
www.labwashers.com 800-991-9380 proinfo@mieleusa.com
From water treatment labs doing trace metal analysis to university tissue culture labs, the Miele G 7883 sets the standard for brilliant results in an under counter design and can be installed into an immediate laboratory area. This washer offers 8 standard and 2 utility wash programs, one custom program, a DI rinse cycle, steam condenser with low ambient heat, and precise sensors. The G 7883 also offers adjustable temperatures up to 93 C for superior cleaning ability. With individually adjustable wash and rinse temperatures, this glassware washer allows you to custom tailor settings to the task at hand. If aggressive cleaning is needed, a high temperature can be set. If not, the temperatures can be lowered, therefore shortening the cycle time. The G 7883 is good for cleaning laboratory glassware pipettes, beakers, volumetric flasks, petri dishes, test tubes, anything up to 18 height. Manufactured to last through 15,000 operating hours with minimal heat loss, the G 7883 is engineered to run at maximum efficiency, and is a great Green Choice for todays labs.
Generally, the more critical the research, the more likely [a lab] will employ an automated washing process.
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WasHeRs
Recently Released WasHeRs FlaskScrubber SW3000 Series
Features a lower spindle rack that holds up to 36 pieces of primarily narrow-neck glassware Includes specialized inserts for pipets, BOD bottles, Petri dishes, culture tubes and small utensils Water and detergent are pumped through spindles to reach innermost areas of glassware Features forced-air drying through spindles to ensure thorough drying inside and out Labconco www.labconco.com
Features rotating spray washing arms and versatile cycle programming Available in single-door or two-door pass-thru configurations Includes a simple touch-control user interface, displaying event, time and temperature Capacity for culture dishes, tubes, slides, pipettes, cylinders, flasks, beakers, bottles, etc.
discussing the most up to date information for the Lab Managers
Scientek www.scientek.net
G7893
A complete washing and drying cycle takes one hour Saves space with a 24-inch footprint Incorporates true HEPA-filtered forced-air drying A cool-down step can be programmed at the end of cycle to ensure glassware is safe to handle Miele www.labwasher.com
WD 290
Dynamic Filling provides resource savings of up to 20% per cycle Each cycle requires 40 liters less water and energy turnover is reduced by 20% Productivity boost of up to 25% can be achieved with DI water preheating option Features full glass doors and chamber illumination so the cleaning process can be monitored Belimed www.belimed.com
Editors Buzz
by Pam Ahlberg
waSHeR ManUfactUReRS
BeliMed Getinge Hotpack Labconco Lancer Miele Scientek SteelCo STERIS Corporation Thermo Fisher Scientific Yamato Scientific America www.belimed.us www.getingeusa.com www.hotpack.com www.labconco.com www.lancer.com www.labwashers.com www.scientek.net www.steelcospa.com www.steris.com www.thermo.com www.yamato-usa.com
This Week in Your Lab
www.labmanager.com/blogs/Editor
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WateR puRification systeMs

PURELAB flex - the future of pure water
Ensure accurate consistent results with ELGAs PURELAB flex. We understand how important it is to use reliable ultrapure and pure water. The PURELAB flex is designed around your needs, delivering accuracy, flexibility and ease of use with an innovative and ergonomic design. ELGAs PURELAB flex is a state-of-the-art water purification system. It integrates in-hand monitoring and accurate pure water dispensing in one unique product. This innovative water purification system has won many awards including the 2010 Instrument Business Outlook Award for Laboratory Equipment Industrial Design.
pRoducts in action
WateR deMands youR Respect: tHeRe is MoRe to pRocuRing puRe WateR tHan investing in tHe RigHt WateR puRification systeM
As instruments have become more sensitive and applications increasingly complex, the demand for high-purity water has also increased. Parts-per-million (ppm) mittee for Clinical Laboratory Standards (NCCLS) has specified three types of water: I, II and III, as well as special-purpose water, depending on their use. Type I refers to the purest water on the scale and is used for most analytical applications, while Type III water is used for general washing. Special-purpose water refers to water that has been treated to remove specific contaminants. When selecting a water purification system, several factors need to be considered. However, according to Bob Applequist, product manager at Labconco, the most important ones are to fit the product to the application and differentiate between the need for pure and ultrapure water. In most cases, the pure water generated from tap water can be used for most applications, while ultrapure water generated from a point-of-use system can be used for applications that have more specific and stringent purification needs. The first-step purification or the system that is used to convert tap water into pure water has to be very efficient, says Bardon. If you have that first step right, converting that pure water into ultrapure water is going to be very easy and consistent. When considering a water purification system, both the quality and the quantity of water have to be taken into account, as well as daily water volume requirements, says Bardon. For labs with variable demands on quality and quantity, flexibility and modularity become very important. The key then is to invest in a flexible system that will meet your needs today and grow with the lab and change with the applications, says Matthew Hammond, global sales and marketing director for ELGA LabWater. After choosing the right system, performing regular, preventative maintenance is equally important. Modern systems have built-in alarms and calibrators that warn customers if certain components are nearing the end of their life cycles. Sample the water routinely to make sure that it doesnt contain impurities that will interfere with your analysis, says Hammond. The level of monitoring can be done daily, weekly or monthly, depending on the stringency of the application. Whatever system you buy, make sure its dynamic, so that the water can recirculate regularly, Hammond adds. Water needs to be kept moving, as still water ends up building biofilms quicker, so look for a system that is easy to sanitize.
5 Earl Court Woodridge, IL 60517 (877) 315-3542 Email: elga.usa@veoliawater.com www.elgalabwater.com
Invest in a flexible system that will meet your needs today and grow with the lab.
is no longer the smallest level of contamination, as users are testing for parts-per-billion (ppb) or parts-per-trillion (ppt) levels. Contaminants in water can consist of particulates, organics, inorganics, microorganisms and pyrogens. Renaud Bardon, director for North American Sales, Lab Water at Millipore, says in the past, people were mainly concerned with ionic contaminants, but today, people are more concerned with organic contaminants, particulates and microorganisms. Eight commonly used methods to purify water are distillation, deionization, reverse osmosis, activated carbon filtration, microporous filtration, ultrafiltration, ultraviolet oxidation and electrodialysis. The National Com-
The water quality conforms to international water standards e.g. CLSI, CLRW, ISO 3696: Grade 1,2,3, ASTM D1193-06, Pharmacopeia USP, EP and JP. The PURELAB flex can be used for analytical and lifescience applications in all pharmaceutical, university, hospital, food and beverage laboratories. The PURELAB flex is easily adaptable to facilitate changes to laboratory design layouts and applications.
Handset designed for todays laboratory

Intuitive to use Ergonomic handset design Clear water purity for absolute confidence as you dispense Handset displays prioritized information shown at all times (system status, TOC, alarm) POU filters for multiple applications to remove endotoxins, DNase, RNase and bacteria Flexible dispensing in four different ways o Variable flow drop by drop or up to 2 liters per minute o Autovolume dispense from 50ml to 60ml and repeat dispensing o Hands free with optional foot pedal o Locked dispense for glassware filling
The PURELAB flex delivers 18.2 M-cm water quality.
Water Purification made easy

Fast and simple sanitization to minimize microbial growth Data capture via USB port for system validation User settings can be customized via the web and uploaded using the USB post in seconds Multiple dispense positioning o Wall, bench, height adjustable arm, hand-held dispensing ELGA is the global laboratory brand name of Veolia Water Solutions and Technologies, the worlds leading water service company.
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pRoducts in action
Recently Released WateR puRification systeMs CRYSTA Series PURELAB flex
Produces high-quality ultrapure water (Type I) and pure water (Type II) CRYSTA 1000 and 2000 models require reverse osmosis or deionized water as input source and can be used with CRYSTA 500 to create a complete purification system Features a timed dispense mode for walk-away, unattended operation
Delivers up to 2 liters per minute of type I, II and III water Flexible dispense tip filtration guarantees microbial purity <1CFU/10ml Water purity maintained by recirculation of purified water through ion exchange and UV purification technologies to the dispense point
Steriflip Vacuum-Driven Filtration Device

For decades, the Steriflip filters innovative design has provided cell biologists with ease of use, speed, and sterility.
290 Concord Road Billerica, Massachusetts Phone : 800-645-5476 www.millipore.com
Aurora Biomed www.aurorabiomed.com
ELGA LabWater www.elgalabwater.com
LaboStar
Produces analytical grade water on demand Features easy push-button operation and quick sanitization regimens Recirculation feature maintains optimal water quality while minimizing bacterial growth Produces up to 18 megohm ASTM water quality
Q-POD Element Unit

Ensures delivery of ultrapure water with very low levels of elemental contamination Features a footswitch for hands-free water delivery Ideal for use in a Class 1000 clean room environment Supplies water in volumes required by the user (up to 1.5 L/min)
Siemens Water Technologies www.siemens.com
Millipore www.millipore.com
wateR pURification SySteM ManUfactUReRS

Aqua Solutions Aries Filterworks Aurora Biomed ELGA LabWater Labconco Millipore Pall Corporation Sartorius Stedim Siemens Thermo Fisher Scientific www.aquaa.com www.arieswater.com www.aurorabiomed.com www.elgalabwater.com www.labconco.com www.millipore.com www.pall.com www.sartorius-stedim.com www.water.siemens.com www.thermoscientific.com
Cell culture is an exacting and time-consuming prerequisite for cell-based assays, which require cells that exhibit reproducible phenotypes and are free of contamination from yeast, bacteria, and other cells. Because microbiological contamination is commonly introduced during liquid transfer steps, minimizing liquid transfer increases cell culture reliability. Speed can also determine cell culture success; the time required to transfer or sterilize sensitive reagents can result in degradation of labile, essential molecular species. Millipores Steriflip filter eliminates liquid transfer and speeds sample handling by integrating a filter into the mouth of a sterile tube to which the original sample tube is directly attached.
Sterilize Quickly With High Protein Recovery

Cell culture reagents and media often contain high concentrations of serum, proteins, and viscous solutions. As a result, filter clogging and protein binding are common challenges. Steriflip filters provide both fast flow and high protein recovery. Sterilizing-grade Steriflip filters are available with either fast-flow Millipore Express Polyethersulfone (PES) or low-protein-binding Durapore polyvinylidene fluoride (PVDF) membranes, and feature 0.22 m or 0.45 m pore sizes.
Isolate Cells Fast For High Viability

For cell isolation applications, researchers can use nylon mesh Steriflip filters, which enable faster separation of large volumes of cellular material, improving recovery and reducing time to collect isolated cells. Four mesh sizes allow for optimal performance in stem cell, cardiomyocyte, neurological and skeletal tissue applications. Nylon mesh Steriflip devices are available in 20 m, 40 m, 60 m, and 100 m sizes.
Fast, Easy, Sterile Cell Culture With Steriflip Filters

The Steriflip filters revolutionizing design has made it a popular tool among cell culturists aiming to eliminate contamination while simultaneously increasing experimental throughput. Filtration using the Steriflip device is straightforward, requiring only three steps: 1. Attach filter to 50 mL sample tube 2. Flip assembly upside down 3. Apply vacuum and recover sample in new tube
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November 2010

Product Resource Guide

Product resource Guide

ProducT focuS analYTical

Product resource Guide 2010

00 PRODUCT FOCUS LiFe SCienCe

Your research demands performance labware. We deliver.

PRODUCT FOCUS baSiC Lab

Thermo Scientific Nalgene cryo vials deliver superior sample protection.

Product Resource Guide 2010

MainSTay OF ORganiC CheMiCaL anaLySiS

6555 Pelican Creek Circle Riverview, FL 33578 866-METROHM (638-7646) www.metrohmusa.com

GC detectors have been evolving rapidly to provide greater sensitivity.

Our IC systems save in so many ways

Product Resource Guide 2010

Product Resource Guide 2010

ReCenTLy ReLeaSeD gC SySTeMS GC-2010 Plus Capillary Clarus 680 GC

Shimadzu Scientific Instruments www.ssi.shimadzu.com

LECO Corporation www.leco.com Inrag www.inrag.ch

LIKE WHAT YOU SEE?

Product Resource Guide 2010

bOOST PReCiSiOn, RePRODUCibiLiTy FOR CRiTiCaL LC MeThODS

Shimadzu Scientific Instruments www.ssi.shimadzu.com

hPlc auToSaMPler ManufacTurerS

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FOR hPLC, WheRe The aCTiOn iS

hPlc coluMn ManufacTurerS

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USeRS DeManD PRODUCTiviTy, QUaLiTy

Middleton, WI Tel: 800-445-7661 Fax: 608-831-4451 www.gilson.com

Its Your Chemistry Make it Personal!

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hPlc SYSTeM ManufacTurerS

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exTenDing viSUaLizaTiOn inTO ThRee DiMenSiOnS

Handheld Digital USB

Helios NanoLab 50 Series DualBeam

JEOL USA www.jeolusa.com

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PaRTiCLe Size anaLyzeRS

eSSenTiaL FOR Many inDUSTRieS, PaRTiCLe Sizing COnTinUeS TO UnDeRgO innOvaTiOn

Beckman Coulter www.beckmancoulter.com

ParTicle Size analYzer ManufacTurerS

Fluid Imaging Technologies www.fluidimaging.com

Product Resource Guide 2010

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SenSiTive, QUanTiTaTive MeTaL anaLySiS

Buck Scientific www.bucksci.com

graphite furnace instruments require less sample than flame AA spectrophotometers.

Analytik Jena www.analytik-jena.com

Product Resource Guide 2010

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PiCking UP STeaM in nOnTRaDiTiOnaL MaRkeTS

ML with DialPath Technology

Exoscan and FlexScan

fTir SPecTroPhoToMeTer ManufacTurerS

Product Resource Guide 2010

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STePChiLD OF vibRaTiOnaL SPeCTROSCOPy COMeS OF age