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4.1 CHROMOSOMES
• 40% 60%
• Long, double stranded Histones
(duplex)
• 150 million (1.5 X
108)nucleotide pairs
• Length ≈ 4cm (coiled to
fit into nucleus)page
360, Biology,7th Edit.,
Campbell.
Sister chromatid
Spindle microtubule
Centromere Kinetochore
Chromosome
• Centromere – point of constriction of the
chromosome containing specific DNA sequence
(condensed area).
• Kinetochore – disk of protein which functions as
attachment site for fibers → assist in cell division.
HMM/SCM1414
Cell Cycle
G1 S G2
Summary:
Phase Events within cell
G1 Intensive cellular synthesis & cell growth
occurs.
Mitochondria, chloroplasts, ER,
lysosomes, Golgi apparatus & vesicles
produced. Nucleolus produces rRNA.
mRNA and tRNA.
Cell produces structural and functional
proteins.
Substances produced to inhibit or
stimulate onset of next stage.
S DNA replication.
Histones synthesized and wind each DNA
strand.
Each chromosome has become two
chromatids.
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(a) INTERPHASE
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G2 :
Chromatin fibres condensed → coil more tightly.
Micotubular apparatus assembled (to move
chromosome to opposite poles of cell).
In animal cells – centriole replicates forming
centrosome.. (None in plants)
(b)PROPHASE
Chromosomes are visible due to high degree of
coiling and condensing of chromatin fibres.
In animal cells: centrioles pair move apart, mitotic
spindles formed between them.
Centrioles produce asters (shorter microtubules
facing the plasma membrane), anchors centrioles to
plasma membrane.
(d) PROMETAPHASE
(d)METAPHASE
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(e)ANAPHASE
(f)TELOPHASE
CYTOKINESIS
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(a) Interphase
(b) Prophase 1
Leptotene:
Chromosomes coil/condense tightly. Homologous
chromosomes are loosely paired aligning gene by
gene.
Zygotene:
Homologous chromosome in the pairing process
(forming tetrad/bivalent// a group of four
chromatids), each tetrad has one or more
chiasmata.
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Pachytene: A B
Synapsis completed – synaptonemal complex
enables crossing over between homologous
chromosomes.
A B
Diplotene:
Homologues are repelling each other
Chiasma appear
Chiasma Formation: (Plural – chiasmata)
X-shaped structure as seen under
light microscope.
Evidence of crossing over. DNA molecules in
Chiasma
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Diakinesis
Bivalent moves to nuclear membrane.
Nuclear membrane begins to break down.
Transcription stops.
Chromosomes recondense.
(c) Metaphase 1
(d) Anaphase 1
(f) Prophase 11
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(g) Metaphase 11
(h) Anaphase 11
Centromeres separate.
Sister chromatids separate and each chromatid
move as a chromosome toward different /
opposite pole.
Chromosomes decondensed.
Nuclear envelope reformed.
Four daughter cells, each with haploid set and
different genetic material, are produced.
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Three checkpoints:
M
checkpoint
G1
checkpoint
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Tumor
(Latin = any swelling)
Inflammation Neoplasia
(Redness & swelling with (Abnormal new growth)
heat & pain)
Benign Malignant
Growth usually slow, (Cancer)
localized and/or Growth often rapid,
encapsulated disorganized, not
confined – invades and
replaces or destroys
adjacent tissues;
metastasizes.
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