Professional Documents
Culture Documents
477
VI
PR
September 1998
Enteric septicemia of catfish tics. ESC was described in a pub- fish such as the danio, green knife
(ESC), caused by the gram nega- lished account in 1979 and the fish, and rosy barb. Other fish
tive bacterium Edwardsiella ictaluri, causative bacterium was species, such as the rainbow trout,
is one of the most important dis- described as a new species in chinook salmon, blue tilapia and
eases of farm-raised channel cat- 1981. European catfish, have been
fish (Ictalurus punctatus). ESC Although recent evidence indi- experimentally infected with the
accounts for approximately 30 cates that ESC may have been pre- bacterium, but natural outbreaks
percent of all disease cases sub- sent in Arkansas as early as 1969, in these species have not been
mitted to fish diagnostic laborato- records from fish diagnostic labo- reported.
ries in the southeastern United ratories indicate that it was not
States. In Mississippi, where chan- prevalent in the industry immedi- Range
nel catfish make up the majority ately following its discovery.
of case submissions, it has been ESC is primarily a pathogen of
Only 26 cases were recorded by channel catfish cultured in the
reported at frequencies as high as the SECFDL between January
47 percent of the yearly total. southeastern United States. The
1976 and October 1979, and ESC disease has been diagnosed from
Economic losses to the catfish occurred in only 8 percent of the
industry are in the millions of dol- catfish production areas in Miss-
total cases reported by the issippi, Arkansas, Alabama,
lars yearly and continue to Mississippi Cooperative Extension
increase steadily with the growth Louisiana, Georgia and Florida. It
Service in 1980 and 1981. Between occurs less frequently in Virginia,
of the industry. 1982 and 1986 the increase in ESC Texas, Idaho, Indiana, Kentucky,
ESC was first recognized as a new incidence was explosive and the California, Arizona and Maryland.
infectious bacterial disease of impact on the catfish industry sig- Natural fish kills in wild popula-
pond-raised channel catfish in nificant. ESC is now known to tions of catfish due to ESC are
1976 through the examination of occur throughout the geographic rare; only two cases are on record.
diseased specimens from Alabama range of the catfish industry.
and Georgia submitted to the Clinical signs and
Southeastern Cooperative Fish Species susceptibility
Disease Laboratory (SECFDL) at diagnosis
Auburn University. The disease The channel catfish is the fish
was similar to another disease of most susceptible to infection by Behavior
catfish caused by the gram nega- Edwardsiella ictaluri, but white cat- Catfish affected with ESC often
tive bacterium Edwardsiella tarda, fish, brown bullhead, and walking are seen swimming in tight circles,
but differed in several characteris- catfish are also susceptible. Blue chasing their tails. This head-chas-
catfish (Ictalurus furcatus) occa- ing-tail, whirling behavior is due
sionally contract ESC but have to the presence of the Edwardsiella
1School of Veterinary Medicine, Louisiana been shown to be resistant to ictaluri in the brain. Affected fish
State University experimental infection. also sometimes hang in the water
2Cooperative Extension Program, Edwardsiella ictaluri has been iso- column with the head up and tail
Kentucky State University lated from diseased ornamental down. In addition, catfish with
ESC tend to stop eating shortly Internal Signs infusion (BHI) agar. Isolates from
after becoming infected. Clear, straw-colored or bloody the internal organs and brain of
fluid is often present in the fishÕs catfish streaked on these media
External Signs take about 2 days at 25 to 30¡ C to
body cavity. The liver typically
ESC-affected catfish frequently has characteristic pale areas of become readily apparent. Growth
have red and white ulcers (rang- tissue destruction (necrosis) or a of Edwardsiella ictaluri often is not
ing from pinhead size to about general mottled red and white detectable at 24 hours. The 48-
half the size of a dime) covering appearance (Fig. 5). Petechial hour cultures are typically com-
their skin (Fig. 1); pinpoint red hemorrhages can be found in the posed of very high numbers of
spots (called petechial hemor- muscles, intestine and fat of the extremely small, punctate, white
rhages) especially under their fish. The intestine is also often colonies. The bacterium should be
heads and in the ventral or belly filled with a bloody fluid. gram negative, weakly motile,
region (Fig. 2); and longitudinal, rod shaped (0.75 x 1.25 µm), oxi-
raised red ÒpimplesÓ at the cra- Diagnosis dase negative, fermentative in
nial foramen between the eyes ESC typically is diagnosed by O/F glucose or glucose motility
(Fig. 3) that can progress into the culture and isolation of the deeps (GMD), triple sugar iron
Òhole-in-headÓ condition. Internal causative bacterium from the (TSI) slant reaction K/A with no
build-up of fluid can lead to a internal organs or brain tissue on H2S, and negative for indole pro-
swollen abdomen and exoph- tryptic soy agar (TSA) with 5 per- duction in tryptone broth.
thalmia (popeye) (Fig. 4). cent sheepÕs blood or brain heart
Figure 2. Petechial hemorrhaging caused by ESC on the Figure 4. The exophthalmia in this channel catfish finger-
ventral surface of a channel catfish. (Photo by John ling was caused by fluid build-up from an infection with
Hawke) Edwardsiella ictaluri bacterium. (Photo by Bob
Durborow)
wet nets and equipment, but
allowing the equipment to air dry
in direct sunlight should be suffi-
cient to kill the bacteria.
ESC occurs within a specific tem-
perature range sometimes referred
to as the ÒESC window.Ó Out-
breaks typically occur in the
spring and fall when water tem-
peratures are between 20 and
28¡C (68 to 82¡F). Mortalities
slow and usually stop outside this
temperature range.
The work reported in this publication was supported in part by the Southern Regional Aquaculture Center through Grant No. 94-38500-0045 from
the United States Department of Agriculture, Cooperative States Research, Education, and Extension Service.