Ph.D., DEGREE (WRITTEN EXAMINATION) 2011.

SYLLABUS Paper-II Name of the candidate Name of the Research Supervisor Topic of the Research : : : : Topic of specialization and Research Problem Mrs Anila Devi. Meruva Prof. K.SATHYAVELU REDDY Insilico studies on Phytochemical compounds from leaf extract of Xanthium indicum on STZ diabetic albino rats

induced Unit-I: Diabetes mellitus a) History and classification of diabetes mellitus b) Patho physiology of type 2 diabetes; risk factors and complications of diabetes c) Insulin and Glucose metabolism: Insulin and Glucose production, Insulin and Glucose utilization.
a)

b)
c)

Unit-II: Introduction to medicinal plant Medicinal importance of Xanthium indicum Methods of extraction: organic solvent extraction, extraction with super critical gases, steam distillation and other extraction methods Phytochemical investigations: Preliminary phytochemical screening, qualitative chemical examination and physical evaluation Unit-III: Antioxidants and Antioxidant enzymes a) Classification of antioxidants and antioxidant enzymes b) Antioxidant defense mechanisms c) Biological importance of antioxidants Unit-IV: High Performance liquid Chromatography and NMR Spectroscopy a) Chromatography: Different types of chromatography b) NMR studies of protein- ligand interactions c) Applications of HPLC and NMR Spectroscopy Unit-V: Docking studies a) Pharmacophore modeling: QSAR methods, Receptor Ligand docking b) Prediction of biomacromolecular binding

c) Energy minimization procedures: Steepest-Descent minmiser, conjugate radient method, Newton-Rapson minimiser ; Use of charges, salvation effects.

References:
1. Concise medical physiology - Sujith K Chaudhuri 2. Handbook of diabetes- Rudy Bilous, Richard Donnelly 3. Diabetes millites: A fundamental and clinical context Jerrold M. Olefsky

-2004
4. All flesh is grass: Plant-Animal relationships Joseph Seckbach 5. Phytochemical techniques Raaman N, 2006 6. Harpers Biochemistry Robert k. Murray. Dryl k. Grannel, peter A. Mayes, Vichor W-Rod well 7. Principles of molecular cardiology Marschall Stevens Runge, Cam Patterson 2005 8. HPLC for pharmaceutical scientists Yuri Kazakevich, Rosario LoBrutto, 2007 9. High Performance Liqid Chromatography in Phytochemical Analysis Wasksmundzka-Hanios, Joseph Sherma , 2007

10. NMR of macromolecules: a practical approach- Gordon Carl Kenmure

Roberts, 1993
11. Protein-ligand interactions: methods and applications- Gerd Ulrich

Nienhaus - 2005
12. Nuclear Magnetic Resonance G.A.Webb 13. Solid-state NMR studies of biopolymers Ann E.McDermott, Tatyana Polenova 14. Biomacromolecules: introduction to structure, function and informatics C.Stan Tsai 15. Molecular modeling: basic principles and applications H.DHoltje, W.Sippl, D.Rognan, G.Folkers 16. Bioinformatics Methods And Applications : Genomics, Proteomics and drug discovery S.C.Rastogi, N.Mendiratta, P.Rastogi

Ph.D., Degree (written) Examination-2010 MODEL QUESTION PAPER Answer any five Questions All questions carry equal marks

Time: 3 Hours 1) Explain in detail the Phytochemical extraction methods

Max.Marks:100

2) Write the economic and medicinal importance of in detail 3) Write in detail classification of diabetes mellitus 4) Give brief account on some medicinal plants which are being used to treat diabetes 5) Write a short note on a) Risk factors of diabetes b) Complications of diabetes 6) Explain in detail the citric acid cycle with reference to Energy generation 7) Give an account of the role of antioxidant enzymes in oxidative stress

8) Write short notes on a) Freeze Drying method b) Microwave drying 9) Write in detailed the classification and production of free radicals 10) Write short notes on a) STZ b) Alloxan