Product Catalogue

1st edition March 2011

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Contents
Page
Introduction to BBI 3

How to Order

Gold Colloid

Gold Conjugates

Silver Colloid

Gold Nanorods - Nexus RodsTM

Gold Nanobeads - Nexus BeadsTM

Microarrays

10

Miscellaneous Products

11

BBI Online a Technical forum a Order online

12

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Introduction
Founded in 1986, BBI is the world leader in the manufacture of high quality gold and silver reagents for research and diagnostic applications. Our policy has always been to provide outstanding products, manufactured and tested with the highest emphasis placed on reliability, sensitivity and reproducibility. In addition, our vast experience enables us to provide our customers with a comprehensive technical support programme. Detailed within this catalogue, our Life Science product range is made up of gold and silver colloid and gold conjugates, as well as our Nexus range comprising gold nanorods and gold nanobeads. We also provide a complete creative conjugation service, developing gold conjugates outside of our catalogued range. We welcome discussion with our customers to consider the possibilities of creating any type of gold conjugate to meet your requirements.

How to order
Orders can be made by sending a Purchase Order to: +44 (0) 29 2074 7242 Or online: www.buybbi.com info@buybbi.com

Technical Support
Take advantage of our technical support programme info@bbigold.com +44 (0) 29 2074 7232

Ongoing Requirements
Contact us to set up a proforma account +44 (0) 29 2074 7232 info@buybbi.com

Larger volumes are available on request

All prices stated are in pounds sterling. All rights reserved BBInternational March 2011.

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Gold Colloid
BBIs unique recipe gold colloid has been market leading for over 25 years due to its superior quality, consistency and performance, offering excellent stability and sensitivity characteristics. There are several advantages of choosing BBIs gold colloid: Lowest available coefficient of variation (CV) The choice of particle size makes it possible to study antigens microscopically Allows multiple labelling of antigens on tissue sections Labelling is completely unequivocal Provides a permanent label Quantitation under the electron microscope is possible due to its particulate nature The optical properties mean there is a variation in colour from red to pink to orange depending on particle size, a property that can be successfully exploited in a range of applications Known to be catalytically active for a range of commercially important reactions

Pricing
Gold Colloid Array Gold Colloid

20ml
37.00 See page 10

100ml
146.00

500ml
487.00

Sizes
Code
EMGC2 EMGC5 EMGC10 EMGC15 EMGC20 EMGC30 EMGC40

Size
2nm 5nm 10nm 15nm 20nm 30nm 40nm

Code
EMGC50 EMGC60 EMGC80 EMGC100 EMGC150 EMGC200 EMGC250

Size
50nm 60nm 80nm 100nm 150nm 200nm 250nm

Gold Colloid Starter Packs

A wide range of particle sizes can be conjugated to proteins and macromolecules, and first time gold users or those trying out a new process may be unsure of which size to purchase. Our gold colloid starter packs provide a range of particle sizes allowing a comprehensive evaluation.

Code
Gold Colloid 5nm/10nm/20nm/40nm Gold Colloid 20nm/40nm/60nm/80nm GCIKITLIFE GCIKITDIAG

Volume
20 ml of each 20 ml of each

Price
131.00 131.00

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Gold Conjugates
A colloidal gold conjugate consists of a suspension of gold particles coated with a selected protein or macromolecule (such as an antibody). BBI has a range of EM grade products that combine different antibodies on the range of particles predominantly 2, 5, 10, 15, 20, 30 & 40nm. BBIs gold conjugates provide the following benefits. Binding to BBIs superior quality gold colloid ensures a very low level of clustering while maintaining high level stability and sensitivity Only highest quality antibodies and proteins are used, meaning consistently reliable products BBIs gold conjugates average 95% singlet particles

Pricing
EM and all Conjugates (except EM. BSA) EM. BSA Conjugates Microarray Conjugates

0.25ml
75.00 53.00 121.00

1ml
224.00 149.00 424.00

Conjugate Key
BSA Bovine Serum Albumin CGC Cationic Colloidal Gold GAF Goat anti Mouse IgG & IgM GAG Goat anti Guinea Pig IgG GAM Goat anti Mouse IgG Fc (Gamma) Specific GAR Goat anti Rabbit IgG GAT Goat anti Rat IgG GFAR Goat F(ab)2 anti Rabbit IgG GMHL Goat anti Mouse IgG (H+L) PAG Protein A RAG Rabbit anti Goat IgG STP Streptavidin

Code
EM. BSA5 EM. BSA10 EM. CGC5 EM. CGC10 EM. CGC15 EM. CGC20 EM. GAF10 EM. GAG5 EM. GAG10 EM. GAM2 EM. GAM5 EM. GAM10 EM. GAR2

Size
5nm 10nm 5nm 10nm 15nm 20nm 10nm 5nm 10nm 2nm 5nm 10nm 2nm

Code
er EM. GAR5 Best Sell er EM. GAR10 Best Sell er EM. GAR15 Best Sell

Size
5nm 10nm 15nm 20nm 30nm 40nm 10nm 5nm 10nm 5nm 10nm 15nm 20nm

Code
EM. PAG5 EM. PAG10 EM. PAG15 EM. PAG20 EM. RAG5 EM. RAG10 EM. RAG15 EM. RAG20 EM. STP2 EM. STP5 EM. STP10 EM.STP15 EM.STP20

Size
5nm 10nm 15nm 20nm 5nm 10nm 15nm 20nm 2nm 5nm 10nm 15nm 20nm

EM. GAR20 EM. GAR30 EM. GAR40 EM. GAT10 EM. GFAR5 EM. GFAR10
er EM. GMHL5 Best Sell er EM. GMHL10 Best Sell er EM. GMHL15 Best Sell EM. GMHL20

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Silver Colloid
The use of silver can potentially lead to improved sensitivity where silver nanoparticles demonstrate a stronger, more distinctive SPR spectrum than the corresponding gold particles. Use of silver in place of gold can potentially result in a more defined wavelength shift, and broadens the range of optical properties available an advantage for applications where multiplexing is needed.

Pricing
Silver Colloid Array Silver Colloid

20ml
37.00 See page 10

100ml
146.00

500ml
487.00

Sizes
Code
EMSC20 EMSC40

Size
20nm 40nm

Code
EMSC60 EMSC80

Size
60nm 80nm

CREATIVE CONJUGATION SERVICE BBI are experts in creative conjugation.

Let us conjugate your antibody to gold colloid. 3ml finished conjugate Technical advice and ongoing support

Antibody Purification

Determine Antibody Titre

Manufacture and Concentrate Conjugate

Talk to us about your requirements Tel: +44 (0) 29 2074 7232 info@buybbi.com

Column Chromatography

Binding Analysis

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Nexus RodsTM
Pricing
Nexus Rods
TM

50 OD-ml
300.00

250 OD-ml
1500.00

Our range of Nexus Rods combines the revolutionary optical, photothermal, and mechanical properties inherent in the gold nanorod structure with a well characterized highly customisable surface. This makes them suitable for directing the bioactivity of gold nanorods or for conjugating them to a wide range of molecules. Resulting in better extinction, absorption, and scattering at tunable wavelengths, BBIs Nexus Rods bring many benefits to optical, photothermal, and material methodology.

Amine-Polymer
Size
550nm 600nm 650nm 700nm 750nm 60-R-PA-550-50 60-R-PA-600-50 60-R-PA-650-50 60-R-PA-700-50 60-R-PA-750-50

(Volume: 1ml)

Neutravidin-Polymer
Size
550nm 600nm 650nm 700nm 750nm 60-R-PN-550-50 60-R-PN-600-50 60-R-PN-650-50 60-R-PN-700-50 60-R-PN-750-50

(Volume: 1ml)

Codes
60-R-PA-550-250 60-R-PA-600-250 60-R-PA-650-250 60-R-PA-700-250 60-R-PA-750-250

Codes
60-R-PN-550-250 60-R-PN-600-250 60-R-PN-650-250 60-R-PN-700-250 60-R-PN-750-250

Biotin-Polymer
Size
550nm 600nm 650nm 700nm 750nm 60-R-PB-550-50 60-R-PB-600-50 60-R-PB-650-50 60-R-PB-700-50 60-R-PB-750-50

(Volume: 1ml)

Methyl-Polymer
Size
550nm 600nm 650nm 700nm 750nm 60-R-PM-550-50 60-R-PM-600-50 60-R-PM-650-50 60-R-PM-700-50 60-R-PM-750-50

(Volume: 1ml)

Codes
60-R-PB-550-250 60-R-PB-600-250 60-R-PB-650-250 60-R-PB-700-250 60-R-PB-750-250

Codes
60-R-PM-550-250 60-R-PM-600-250 60-R-PM-650-250 60-R-PM-700-250 60-R-PM-750-250

Carboxyl-Polymer
Size
550nm 600nm 650nm 700nm 750nm 60-R-PC-550-50 60-R-PC-600-50 60-R-PC-650-50 60-R-PC-700-50 60-R-PC-750-50

(Volume: 1ml)

Codes
60-R-PC-550-250 60-R-PC-600-250 60-R-PC-650-250 60-R-PC-700-250 60-R-PC-750-250

BBI FACT: Custom Sizes

In addition to our catalogue products, BBI are able to manufacture gold particles to custom diameters, up to 600 nm.

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Nexus BeadsTM
Pricing
Nexus Beads
TM

50 OD-ml
300.00

250 OD-ml
1500.00

BBIs range of Nexus Beads are manufactured using a proprietary method that results in exact and highly monodisperse sizes and shapes, indcluding a covalent bonded polymer cage with carboxyl, methyl, biotin, or neutravidin terminal groups. This formulation has been optimized for in vitro use.

Amine-Polymer
Size
5nm 10nm 20nm 30nm 40nm 50nm 60nm 70nm 80nm 90nm 100nm 60-S-PA-5-50 60-S-PA-10-50 60-S-PA-20-50 60-S-PA-30-50 60-S-PA-40-50 60-S-PA-50-50 60-S-PA-60-50 60-S-PA-70-50 60-S-PA-80-50 60-S-PA-90-50 60-S-PA-100-50

(Volume: 1ml)

Biotin-Polymer
Size
5nm 10nm 20nm 30nm 40nm 50nm 60nm 70nm 80nm 90nm 100nm 60-S-PB-5-50 60-S-PB-10-50 60-S-PB-20-50 60-S-PB-30-50 60-S-PB-40-50 60-S-PB-50-50 60-S-PB-60-50 60-S-PB-70-50 60-S-PB-80-50 60-S-PB-90-50 60-S-PB-100-50

(Volume: 1ml)

Codes
60-S-PA-5-250 60-S-PA-10-250 60-S-PA-20-250 60-S-PA-30-250 60-S-PA-40-250 60-S-PA-50-250 60-S-PA-60-250 60-S-PA-70-250 60-S-PA-80-250 60-S-PA-90-250 60-S-PA-100-250

Codes
60-S-PB-5-250 60-S-PB-10-250 60-S-PB-20-250 60-S-PB-30-250 60-S-PB-40-250 60-S-PB-50-250 60-S-PB-60-250 60-S-PB-70-250 60-S-PB-80-250 60-S-PB-90-250 60-S-PB-100-250

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Nexus BeadsTM
Carboxyl-Polymer
Size
5nm 10nm 20nm 30nm 40nm 50nm 60nm 70nm 80nm 90nm 100nm 60-S-PC-5-50 60-S-PC-10-50 60-S-PC-20-50 60-S-PC-30-50 60-S-PC-40-50 60-S-PC-50-50 60-S-PC-60-50 60-S-PC-70-50 60-S-PC-80-50 60-S-PC-90-50 60-S-PC-100-50 (Volume: 1ml)

Methyl-Polymer
Size
5nm 10nm 20nm 30nm 40nm 50nm 60nm 70nm 80nm 90nm 100nm 60-S-PM-5-50 60-S-PM-10-50 60-S-PM-20-50 60-S-PM-30-50 60-S-PM-40-50 60-S-PM-50-50 60-S-PM-60-50 60-S-PM-70-50 60-S-PM-80-50 60-S-PM-90-50 60-S-PM-100-50

(Volume: 1ml)

Codes
60-S-PC-5-250 60-S-PC-10-250 60-S-PC-20-250 60-S-PC-30-250 60-S-PC-40-250 60-S-PC-50-250 60-S-PC-60-250 60-S-PC-70-250 60-S-PC-80-250 60-S-PC-90-250 60-S-PC-100-250

Codes
60-S-PM-5-250 60-S-PM-10-250 60-S-PM-20-250 60-S-PM-30-250 60-S-PM-40-250 60-S-PM-50-250 60-S-PM-60-250 60-S-PM-70-250 60-S-PM-80-250 60-S-PM-90-250 60-S-PM-100-250

Neutravidin-Polymer
Size
5nm 10nm 20nm 30nm 40nm 50nm 60nm 70nm 80nm 90nm 100nm 60-S-PN-5-50 60-S-PN-10-50 60-S-PN-20-50 60-S-PN-30-50 60-S-PN-40-50 60-S-PN-50-50 60-S-PN-60-50 60-S-PN-70-50 60-S-PN-80-50 60-S-PN-90-50 60-S-PN-100-50

(Volume: 1ml)

Codes
60-S-PN-5-250 60-S-PN-10-250 60-S-PN-20-250 60-S-PN-30-250 60-S-PN-40-250 60-S-PN-50-250 60-S-PN-60-250 60-S-PN-70-250 60-S-PN-80-250 60-S-PN-90-250 60-S-PN-100-250

BBI FACT: Nexus

The absorption and scattering sections of Nexus Beads can be up to two orders of magnitude greater than nanoparticles

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Microarray Gold & Silver Colloid

In the Microarray market, gold with its unique properties has emerged as an alternative to fluorescence detection. BBInternationals Microarray gold is the ideal solution for overcoming many of the problems associated with fluorescence detection methods, including low sensitivity, signal intensity, reduction due to photochemical bleaching, quenching and expensive image analysis systems.

Key Features
Highly Specific Reproducible Stable Highly Sensitive Reliable

Code
ArrayGC2 ArrayGC5 ArrayGC10 ArrayGC15 ArrayGC20 ArrayGC30 ArrayGC40 ArrayGC50 ArrayGC60 ArrayGC80 ArrayGC100 ArrayGC150 ArrayGC200 ArrayGC250

Size
2nm 5nm 10nm 15nm 20nm 30nm 40nm 50nm 60nm 80nm 100nm 150nm 200nm 250nm

Volume
20ml 20ml 20ml 20ml 20ml 20ml 20ml 20ml 20ml 20ml 20ml 20ml 20ml 20ml

Perfectly Spherical Shapes (95 %) Low CV (<8%)

Code
ArraySC20 ArraySC40 ArraySC60 ArraySC80

Size
20nm 40nm 60nm 80nm

Volume
20ml 20ml 20ml 20ml Microarray Gold Colloid Microarray Silver Colloid

Pricing

20ml
110.00 110.00

10

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Miscellaneous Products
Pricing
Silver Kit EM/LM (15ml) Test Strips (Silver Kits) Blotting Silver (250ml/kit) Unicryl (250ml) Protogold (500ml/kit) Test Strips (Protogold) Biobond (20ml) Biomount (100ml) BSA 10g (powder) Fish Gelatin (10ml) Tween 20 (10ml) Raman Spec Gold Colloid (150ul) Raman Spec Silver Colloid (150ul)

20ml Code
SEKL15 SETS10 SEKB250 BA250 PRO500 SETPG10 BB20 BM100 BSA10 GEL10 T20 GC.150ul SC.150ul

100ml Price
78.00 20.00 107.00 146.00 107.00 20.00 36.00 25.00 39.00 20.00 9.00 182.00 182.00

Gold Colloid
Starter Pack
Start your evaluation of varying colloid sizes with this versatile pack
First time gold users or those trying out a new process may be unsure of which size to purchase. Our gold colloid starter pack provides a range of particle sizes to allow a comprehensive evaluation, comprising 20ml of each size.

save over
on list price

10%

5nm 10nm 20nm 40nm

See page 4 to order

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11

BBI Online
BBIs Technical Forum
Visit BBIs forum and get involved in all the latest Life Science discussions!

Technical support New product ideas Industry knowledge Technical questions

R&D ideas Interaction with fellow researchers Your feedback

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