Code No: RR322306 Set No.

1
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
PROTEIN ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Write short notes on:

(a) Edman degradation

(b) Identifying Amino terminal and Carboxy terminal residues. [8+8]

2. Justify the statement that “tertiary structure is more organized than secondary
structure”. [16]

3. Define post-translational Modification? Explain Post-translational modification by

alteration of chain termini? [16]

4. Describe the zinc finger and their function. [16]

5. Name the membrane protein which is present in Halobacterium salinarium ? Ex-

plain about mechanisms of light driven proton pump in Bacteriorhodopsin? [16]

6. Discuss the structure of Insulin and describe its functions. [16]

7. Write about Pattern databases for storing information about protein families and
motifs? [16]

8. (a) What do you mean by protein design?

(b) What are the goals of protein design? [8+8]

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Code No: RR322306 Set No. 2
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
PROTEIN ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe the characterization of protein structure? [16]

2. Describe the role of hydrophobic forces in tertiary structure. [16]

3. (a) What are chaperons?

(b) Describe types of chaperons and their role in folding of protein. [8+8]

4. Describe the zinc finger and their function. [16]

5. Describe the properties of three classes of Membrane proteins, how they differ from
one other and how they vary among themselves? [16]

6. Describe the sequence of events following the absorption of photon of light by

photosynthetic reaction center pigment of PS-II. Describe the comparable events
in PS-I. How are two photosystems linked to one another? [16]

7. Write a note on Curated family databases for Protein? [16]

8. (a) What do you mean by protein design?

(b) What are the goals of protein design? [8+8]

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Code No: RR322306 Set No. 3
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
PROTEIN ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain Secondary structure prediction from protein sequences? [16]

2. Describe the role of hydrogen bonds in tertiary structure of protein. [16]

3. Describe Posttranslational modifications by proteolytic cleavage. [16]

4. Explain in detail about the different Transcription factors that contains Zinc as an
essential element of their DNA binding domains? [16]

5. (a) What are proteases.

(b) Describe different classes of proteases with examples.
(c) Explain the action of serine proteases in details. [5+6+5]

6. Explain the process of recognition of foreign molecule by Immunoglobulins. [16]

7. Write a note on engineering complex human proteins by large scale mammalian cell
culture? [16]

8. Explain the importance of protein designing in the field of Drug designing. [16]

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Code No: RR322306 Set No. 4
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
PROTEIN ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Discuss about General properties of Proteins Secondary structure? [16]

2. Write short notes on:

(a) Myoglobin
(b) Globular proteins [8+8]

3. Justify statement “Final structure of protein is not determined by Gene structure

but by the primary structure of protein”. [16]

4. Write short notes on:

(a) Zinc fingers

(b) Leucine zippers [8+8]

5. Name the membrane protein which is present in Halobacterium salinarium ? Ex-

plain about mechanisms of light driven proton pump in Bacteriorhodopsin? [16]

6. (a) What are Immunoglobulins.

(b) Describe the structure of Immunoglobulin.
(c) Describe the various classes of Immunoglobulins and their properties.
[5+5+6]

7. Write short notes on:

(a) PROSITE
(b) BLOCKS [8+8]

8. (a) What do you mean by protein design?

(b) What are the goals of protein design? [8+8]

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