EVALUATION OF GENOTOXIC POTENTIAL AND ANTI-GENOTOXIC OF AQUEOUS EXTRACT OF FRUIT OF Myrciaria dubia PLANT AMAZON h. b. k.

(McVough) (CAMU-CAMU) Silva FC1,2, Picada JN, Romo NF1,2, Viana RN1,2, Meneguetti DUO1,2, Daudt C1,2, Schons S2, Letcia T2, Pelenz D1,2, Souza AC1,2, and Pereira P 1 - Graduate Program in Genetics and Applied Toxicology, Lutheran University of Brazil, Canoas, RS, Brazil. 2 - Applied Toxicological Genetics Laboratory, CEULJI / ULBRA, Ji-Paran, RO, Brazil. e-mail: fcsbiologicalscience@gmail.com keywords: Myrciaria dubia, antioxidant, acute and subacute genotoxicity The Amazon region is rich in endemic forest species, with the potential to be exploited in the form of drugs and foods. However, there is little knowledge about its chemical, biological and toxicological properties. Scientific evidence indicates a significant relationship between treatment with extracts of fruit and a lower incidence of reactive oxygen species (ROS), and therefore reduction in DNA damage. In DNA, the ROS producing series of injuries and damaged deoxyribose bases, causing single chain breaks, apurinic sites and creating apirimidcs and cross-links between DNA and proteins. The aim of this study was to evaluate the genotoxicity of aqueous crude extract of camu camu fruit, at concentration of 100%, 50% and 25% after acute and subacute treatments in samples of blood, liver and kidney, collected after oral administration in Swiss Webster mice. To examine the genotoxicity, we used the alkaline comet assay, in addition, was evaluated in vitro the potential capacity antigenotoxic extract on blood cells, using as a genotoxic agent H2O2. Under the experimental conditions, in acute treatment, the extract did not show genotoxicity in blood cells for both sexes in any of the concentrations used in this experiment. For kidney and liver, only the groups treated with the extract concentration of 100%, showed a level of genotoxicity different of the control group (p < 0.05). Even for the acute treatment, a reduction in the extent of DNA damage caused by H2O2 in blood cells, with results relevant to 50% concentration, no significant differences regarding gender. For treatment of subacute and the results show that the aqueous extract of camu camu also had no genotoxicity in blood cells for both sexes in any of the concentrations. For kidney and liver tissues, we found that statistically significant differences among groups at the three concentrations in comparison of the control group (p < 0.05) without differences in relation to sex. Given this, it is concluded that the investigation of acute and subacute toxicity of aqueous extract of camu camu fruit has shown that it is not genotoxic to blood cells, when administered orally in mice and relatively genotoxic to cells of the liver and kidney tissues. Supported by: CULJI/ULBRA