CURRICULUM VITAE Dr.

RAJESH KUMAR
C/O Dr. Hemalatha R. (Head & Deputy Director), Dept. of Microbiology & Immunology, NIN, Hyderabad-500007 (Andhra Pradesh)

: +919248692698 Email & Chat: rajesh.mbu@gmail.com

OBJECTIVE Looking for a permanent and challenging position in a quality research environment that would allow me to utilize my skills for human wellness through scientific approaches. ACADEMICS

ICMR-Post Doctoral Fellow (2010-2012) NATIONAL INSTITUTE OF NUTRITION, HYDERABAD, INDIA Doctoral Degree Dairy Microbiology (2005-2009) NATIONAL DAIRY RESEARXH INSTITUTE, KARNAL, INDIA Post Graduation Microbiology (2003-2005) BUNDELKHAND UNIVERSITY, JHANSI

RESEARCH/WORK EXPERIENCE

1. Organisation: Dept. of Microbiology & Immunology, NIN, Hyderabad Duration: May 01, 2010 April 30, 2012 (01 Year extendable). Designation: ICMR-POST-DOCTORAL FELLOW, working on rDNA techniques, Real-Time qPCR, in-vivo & in-vitro experiments, Cell culturing, Microencapsulation, Metagenomics, expression of sitespecifically engineered genes and purification of recombinant and native proteins etc. Area of research: Molecular Characterization of Reshuffled Bile Salt Hydrolase (Bsh) and Effect of Dietary Inclusion of Bsh+ and Bsh- Indigenous Probiotic Lactobacillus plantarum strains of Human Origin on Cholesterol Metabolism of Rats under the supervision of Dr. Hemalatha R. (Head & Deputy Director). 2. Organisation: Proteomics and Structural Genomics Lab, NDRI, Karnal Duration: August 07, 2009 April 30, 2010 Designation: RESEARCH ASSOCIATE, worked on cloning, expression, microbial fermentation, immobilization and purification of recombinant proteins like aminopeptidase, carboxylpeptidase, antimicrobial peptides etc. Area of research: Novel Approaches for Production of Nutraceuticals from Milk and Indian Herbs for their Potential Use in Functional Dairy Foods under the supervision of Dr. J.K. Kaushik (Co-PI) 3. Organisation: Molecular Biology Unit, D.M Division, NDRI, Karnal Duration: August 2005 July 2009 Designation: Ph.D SCHOLAR, worked on screening of lactobacilli, Development of Bsh assays, cloning, sequence analysis, expression and purification of recombinant and native Bsh protein (Batch and continuous culturing). Carried out in-vivo & in-vitro trials for anti-hypercholesterolemic effect of Bsh+ probiotic Lactobacillus in SD rats. Area of research: Molecular cloning, sequence analysis and expression of bile salt hydrolase (Bsh) from a potential indigenous probiotic Lactobacillus of human origin under the supervision of Dr. V.K. Batish (Former Head & Er. Scientist).

TECHNICAL SKILLS Purification and characterization of recombinant and native proteins with Gel filtration, Affinity, Ionexchange, Hydrophobic interaction chromatography on FPLC system (Pharmacia & Akta Purifier 10), Ultrafiltration, Rotophor (Isoelectric focussing), denaturing and nondenaturing PAGE. Localization and identification of proteins by Western blotting and Zymograms. Recombinant protein production in Applikon bioreactor (3 L). Cloning, characterization, sequence analysis of gene sequences and optimization of recombinant protein expression. RT-qPCR, RT-PCR, PCRs (Bsh/Species/Genus/Gradient/RAPD), agarose gel electrophoresis. Nucleotide and amino acid sequence submission to NCBI. Efficient use of BLAST, FASTA, Clustal W implemented in Geneious Pro (4.6.4) for sequence analysis, characterization and Primer designing. Nucleic acids isolation and purification. Optimization of different Colorimetric Assays. Anaerobes (Bacterial) culturing in MK3 Anaerobic Workstation (Don Whitley) and in Applikon Bioreactor (3L). Routinely designing and performing experiments on rats (SD & Wistar) and mice models for establishing the role of dietary inclusion of prebiotics, probiotics (immobilized and non-immobilized), synbiotics, neutraceuticals and health foods on health benefits like low serum cholesterol, anti-diabetic and anti-colitis effects. Maintenance and Experimental handling (Survival bleeding, faecal analysis, Dissection etc) of rats & mice for hypocholesterolemic studies. Plasma HDL-, LDL-, VLDL-, Total Cholesterol and TG estimation. Interpretation of data by statistical analysis. Bacterial microencapsulation/immobilization, maintenance, enumeration, preservation and propagation of bacteria from diverse nature of samples. Characterization of Lactobacillus strains for various in-vitro probiotic attributes, efficient handling of all kind of microbiological work.

RESPONSIBILITIES Preparation of ICB (International Competitive Bidding) bid documents and technical specifications for purchasing instruments like Fermentor, Anaerobic Chamber, Vacuum Concentrator, Multi-plate reader, Refrigerated centrifuges, Microfluidizer, Water Purification System, Gel-Documentation System, Incubator Shaker, HPLC, Inverted Fluorescent Microscope etc. for International Competitive Bidding (ICB). Demonstration of laboratory to national and foreign visitors. Maintenance of laboratory and its instruments. Computer skills Operating systems: Windows 7, Vista, XP, 2000 and 98. Software tools: MS office: Others: Publications: Research Article:

Geneious Pro (5.6), DNA Star, Gencheck, Sequin, SYSTAT etc. MS Word, MS Excel, MS PowerPoint. (2000, 2003 & 2007). Installation, maintenance/troubleshooting of softwares and Windows.

Kumar, R., Grover, S., Mohanty, A.K. and Batish, V.K. 2010. Molecular cloning and sequence analysis of bile salt hydrolase (bsh) gene from L. plantarum MBUL90 strain of human origin. Food Biotech. 24 (3): 215-226. (IF: 0.7) Kumar, R., Grover, S. and Batish, V.K. 2011. Hypocholesterolemic Effect of Dietary Inclusion of Two Putative Probiotic Bile salt hydrolase (Bsh) Producing Lactobacillus plantarum strains in SpargueDawley Rats British Journal of Nutrition. 105: 561-573. (IF: 3.446). In Communication

Kumar, R., Grover, S., Kaushik, J.K. and Batish, V.K. Transposon mediated insertional inactivation of bile salt hydrolase (bsh) gene indigenous Lactobacillus plantarum strains Lp77 and Lp20 of Indian origin. Under review in Journal of Molecular Microbiology & Biotechnology. (IF: 2.66).

Kumar, R., Grover, S. and Batish, V.K. Bile Salt Hydrolase (Bsh) Activity as Functional Probiotic Biomarker: In Vitro Selection of Putative Bile Salt Hydrolyzing and Cholesterol Lowering Probiotic Lactobacillus Strains. Under review in Journal of Biomedicine & Biotechnology. (IF: 1.77). Kumar, R., Grover, S. and Batish, V.K. Molecular Typing by Specific Primed-PCR Assays of Putative Probiotic Indigenous Lactobacillus plantarum strain Lp91 of Human Origin. Communicated in Probiotics & Antimicrobial Proteins. (IF: 1.571). Book Chapters

o Kumar, R., Singh, S., Grover, S., Hemalatha, R. and Batish, V.K.. Probiotics for better cardiovascular health. Accepted by IK international publisher, New Delhi for the Book entitled Microbes in the service of mankind: Tiny bugs with huge impact. o Kumar, R., Grover, S. and Batish, V.K. Bile Salt Hydrolase (Bsh) Activity as a Potential Molecular Biomarker for Probiotic Functionality. Under review in Intech Open Access Publisher for the Book project on Genetic Diversity. Abstracts: (07) Screening and Quantification of Bile Salt Hydrolase (Bsh) Activity of Indigenous Lactobacilli: Selection and Application of Probiotic Lactobacillus strain for Reducing Plasma-Cholesterol Levels in Rat Model in 8th International Conference on Functional Food for Chronic Diseases at Las Vegas USA (March 15-17, 2011). Authors: Rajesh Kumar, Sunita Grover and V.K. Batish. Anti-hypercholesterolemic effect of gut microbiota modulation by bile salt hydrolase (Bsh) active indigenous probiotic Lactobacillus plantarum dietary intervention on Spargue-Dawley rats in IPS2010 held at National Institute of Nutrition, Hyderabad (December 14-16, 2010). Authors: Rajesh Kumar, Sunita Grover, Hemalatha R. and V.K. Batish. Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous probiotic Lactobacillus plantarum strain of human origin in 5th Probiotics and Prebiotics and New Foods held at University of Urbania, Rome (Sep 13-15, 2009). Authors: Rajesh Kumar, Sunita Grover and V.K. Batish. Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous probiotic Lactobacillus plantarum strain of human origin in International Conference on Microbial Biotechnology held at Punjab University, Chandigarh (March 03-04, 2009). Authors: Rajesh Kumar, Sunita Grover, A.K. Mohanty and V.K. Batish. Molecular cloning, sequence analysis and expression of bile salt hydrolase (Bsh) gene from potential indigenous probiotic Lactobacillus plantarum isolates of human origin in International Probiotic Conference held at Slovakia, Germany (June 04-07, 2008). Authors: Rajesh Kumar, A.K. Mohanty, Sunita Grover and V.K. Batish. Molecular cloning of bile salt hydrolase gene from a probiotic L. plantarum of human origin in 2nd International Conference on Trends in Cell and Molecular Biology at Jawaharlal Nehru university (JNU), Delhi (Jan 06-08, 2008). Authors: Rajesh Kumar, Sunita Grover and V.K. Batish. Bile Salt Hydrolase activity of some potential indigenous probiotic lactobacilli of human origin in International Conference on Traditional Dairy Foods held at NDRI, Karnal (Nov 14-17, 2007). Authors: Rajesh Kumar and V.K. Batish. Acession nos: (24) EU 477374; EU770692; EU784663; EU836149; EU822811; EU887663; FJ179486; FJ179487; GQ922598; GQ922599; GQ922600; GQ922601; GQ922602; HQ418479; HQ418480; HQ418481; HQ418482; ACG59259; ACF35257; ACF05268; ACF04740; ACA49878; ACF35782; ACG76118. Authors: Rajesh Kumar, Sunita Grover, A.K. Mohanty, J.K. Kaushik. V.K. Batish. Dissertation Training ( March 2005 - June 2005) N.D.R.I KARNAL. India Project Title: Optimization of ultrafiltered retentate for manufacturing of CHAKKA by defined strain starters under the supervision of Dr. S.K. Tomar (Sr. Scientist). Summer Training ( June 2004 July 2004) MOHAN MEAKINS LTD, GHAZIABAD (U.P) DABUR INDIA LTD, SAHIBABAD (U.P) SCHOLARLY ACTIVITY Oral Presentation - Anti-hypercholesterolemic effect of gut microbiota modulation by bile salt hydrolase (Bsh) active indigenous probiotic Lactobacillus plantarum dietary intervention on Spargue-Dawley rats in International conference Pharmacology and Translational Research-2010 held at National Institute of

Nutrition, Hyderabad (December 14-16, 2010). Authors: Rajesh Kumar, Sunita Grover, Hemalatha R. and V.K. Batish.

Poster Presentations Anti-hypercholesterolemic effect of gut microbiota modulation by bile salt hydrolase (Bsh) active indigenous probiotic Lactobacillus plantarum dietary intervention on Spargue-Dawley rats in IPS-2010 held at National Institute of Nutrition, Hyderabad (December 14-16, 2010). Authors: Rajesh Kumar, Sunita Grover, Hemalatha R. and V.K. Batish. Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous probiotic Lactobacillus plantarum strain of human origin in International Conference on Microbial Biotechnology held at Punjab University, Chandigarh (March 03-04, 2009). Authors: Rajesh Kumar, Sunita Grover, A.K. Mohanty and V.K. Batish. Molecular cloning of bile salt hydrolase gene from a probiotic L. plantarum of human origin in 2nd International Conference on Trends in Cell and Molecular Biology at Jawaharlal Nehru university (JNU), Delhi (Jan 06-08, 2008). Authors: Rajesh Kumar, Sunita Grover and V.K. Batish. Bile Salt Hydrolase activity of some potential indigenous probiotic lactobacilli of human origin in International Conference on Traditional Dairy Foods held at NDRI, Karnal (Nov 14-17, 2007). Authors: Rajesh Kumar and V.K. Batish.

Awards - ICMR-Post Doctoral Fellowship (2010-2012) has been awarded by Indian Council of Medical Research (ICMR). - Institutional Senior Research Fellowship awarded during Ph.D programme (Aug 2005- Feb 2009) through Indian Council of Agricultural Research. - Awarded best (Ist) poster presentation on Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous probiotic Lactobacillus plantarum strain of human origin in International Conference on Microbial Biotechnology held at Punjab University, Chandigarh (March 03-04, 2009). - Awarded second best poster presentation on Bile Salt Hydrolase activity of some potential indigenous probiotic lactobacilli of human origin in International Conference on Traditional Dairy Foods held at NDRI, Karnal (Nov 14-17, 2007). Conferences - Actively Participated in 45th AMI conference held at NDRI Karnal (Nov 23 25, 2004).

EXTRA CURRICULAR ACTIVITY

Delivered presentation and Lectures for students of DNA/VPNR clubs sponsored by DBT, Govt of India. Actively participated in as a leading member in annual sports held at NDRI, Karnal (2007, 2008 & 2009).

REFERENCES Dr. V.K Batish (vkbatish@gmail.com) - Former Head & Er. Scientist, Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana, India Dr. Hemalatha R. (rhemalathanin@yahoo.com) - Head & Deputy Director, Microbiology & Immunology Department, National Institute of Nutrition, Hyderabad, Andhra Pradesh, India Dr. Sunita Grover (sungro@gmail.com) - Pr. Scientist, Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana, India Dr. J.K. Kaushik (jaikr1@gmail.com) - Sr. Scientist, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

Dr. A.K. Mohanty (ashokmohanty1@gmail.com) - Sr. Scientist, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

DECLARATION: I hereby declare that the information furnished and entries made in this resume, by me are true to the best of my knowledge and belief and nothing has been concealed/distorted.

Date: 2011-06-20 Place: Hyderabad

(RAJESH KUMAR)