MoBiTec Expands Its Selection of High Quality Gene Expression Systems for

Bacillus megaterium

Based on its already successful protein expression systems for Bacillus megaterium, MoBiTec
GmbH introduces a new family of protein expression vectors, the hp-Expression Systems, for high
yield expression in the well-established GRAS organism Bacillus megaterium. This new system
complements the existing non-E. coli expression systems for B. megaterium, Bacillus subtilis,
Lactococcus lactis and yeast. Further non-E. coli expression systems are in preparation.

Goettingen, Germany, June 09, 2011 --(PR.com)-- MoBiTec GmbH, leading provider of non-Escherichia
coli protein expression systems, expands its series of expression vector systems suitable for protein
expression in Bacillus megaterium.

Bacillus megaterium is a well established and preferred host for heterologous gene expression and an
excellent alternative to the widely used working horse Escherichia coli. B. megaterium is a GRAS
organism (Generally Regarded As Safe) and does not produce toxic compounds like lipopolysaccharides
which makes the purification of produced proteins less tedious and time consuming. The protein yield
obtained with the optimized high performance hp vector systems is up to 10 times enhanced compared to
protein expression with the respective basic plasmid.

hp vectors are available for intracellular expression, without or with affinity tag for convenient
purification:

· pC-His1623hp, E. coli/B. megaterium shuttle vector, C-terminal 6xHis-tag

· pN-His1623hp, E. coli/B. megaterium shuttle vector, N-terminal 6xHis-tag
· p3STOP1623hp, E. coli/B. megaterium shuttle vector

as well as for secretion with 2 different signal peptides:

· pSPLipA-hp, E. coli/B. megaterium shuttle vector with signal sequence

· pSPYocH-hp, E. coli/B. megaterium shuttle vector with signal sequence

Bacillus megaterium protoplasts specifically optimized for transformation

The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W.
Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to
an optimized protocol resulting in the highest transformation efficiencies.

For secretion vectors they offer protoplasts of strain MS941. Both strains are mutants of DSM319,
whereby WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major
extracellular protease NprM.

· Bacillus megaterium protoplasts, ready for transformation (strain WH320)

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· Bacillus megaterium protoplasts, ready for transformation (strain MS941)

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers
research tools for molecular and cell biology. Products include two- & one-hybrid systems for in vivo
detection of molecular interactions, Lambda PS genomic libraries, DNA vectors for cloning and
expression, kits for expression and purification of recombinant proteins, cell transfection reagents and cell
culture tools, immobilized and soluble enzymes, many products for genomics and proteomics research,
numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity
chromatography products, gel analysis tools as well as general laboratory equipment. In parallel to its
own product lines, MoBiTec distributes products from several international companies in Germany.
MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by
distributors.

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Contact Information:
MoBiTec GmbH
Arne Schulz
+49-551-70722-0
info@mobitec.de
www.mobitec.com

Online Version of Press Release:

You can read the online version of this press release at: http://www.pr.com/press-release/330029

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